Starting /dee2/code/volunteer_pipeline.sh SRR26075398 current disk space = 3051237797888 free memory = 1448751084 SRR26075398 SRAfilesize 4c43026a895d86645b1ebc2de490c4aa SRR26075398.sra SRR26075398.sra file validated SRR26075398 is paired end SRR26075398 is conventional basespace SRR26075398 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075398_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6355 37.0 37.0 37.0 37.0 37.0 2 36.6165 37.0 37.0 37.0 37.0 37.0 3 36.735 37.0 37.0 37.0 37.0 37.0 4 36.697 37.0 37.0 37.0 37.0 37.0 5 36.705 37.0 37.0 37.0 37.0 37.0 6 36.678 37.0 37.0 37.0 37.0 37.0 7 36.663 37.0 37.0 37.0 37.0 37.0 8 36.716 37.0 37.0 37.0 37.0 37.0 9 36.6605 37.0 37.0 37.0 37.0 37.0 10-14 36.6828 37.0 37.0 37.0 37.0 37.0 15-19 36.658 37.0 37.0 37.0 37.0 37.0 20-24 36.632099999999994 37.0 37.0 37.0 37.0 37.0 25-29 36.497299999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.4568 37.0 37.0 37.0 37.0 37.0 35-39 36.4256 37.0 37.0 37.0 37.0 37.0 40-44 36.4028 37.0 37.0 37.0 37.0 37.0 45-49 36.33540000000001 37.0 37.0 37.0 37.0 37.0 50-54 36.3091 37.0 37.0 37.0 37.0 37.0 55-59 36.270799999999994 37.0 37.0 37.0 37.0 37.0 60-64 36.225199999999994 37.0 37.0 37.0 37.0 37.0 65-69 36.17659999999999 37.0 37.0 37.0 37.0 37.0 70-74 36.178 37.0 37.0 37.0 37.0 37.0 75-79 36.080200000000005 37.0 37.0 37.0 37.0 37.0 80-84 36.0815 37.0 37.0 37.0 37.0 37.0 85-89 35.975199999999994 37.0 37.0 37.0 37.0 37.0 90-94 35.959199999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.93579999999999 37.0 37.0 37.0 37.0 37.0 100-104 35.897299999999994 37.0 37.0 37.0 37.0 37.0 105-109 35.7933 37.0 37.0 37.0 37.0 37.0 110-114 35.6223 37.0 37.0 37.0 37.0 37.0 115-119 35.6702 37.0 37.0 37.0 37.0 37.0 120-124 35.677899999999994 37.0 37.0 37.0 37.0 37.0 125-129 35.5427 37.0 37.0 37.0 37.0 37.0 130-134 35.422000000000004 37.0 37.0 37.0 37.0 37.0 135-139 35.369 37.0 37.0 37.0 34.6 37.0 140-144 35.2841 37.0 37.0 37.0 32.2 37.0 145-149 35.2126 37.0 37.0 37.0 32.2 37.0 150-151 35.028 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 1.0 21 0.0 22 1.0 23 0.0 24 3.0 25 4.0 26 3.0 27 10.0 28 19.0 29 24.0 30 34.0 31 50.0 32 34.0 33 86.0 34 172.0 35 480.0 36 2879.0 37 198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.90235353029544 17.100650976464696 8.13720580871307 39.85978968452679 2 18.55 13.925 34.725 32.800000000000004 3 17.299999999999997 17.825 26.8 38.074999999999996 4 19.35 20.200000000000003 25.75 34.699999999999996 5 24.099999999999998 25.85 24.85 25.2 6 22.425 33.475 22.7 21.4 7 17.2 27.725 37.724999999999994 17.349999999999998 8 17.95 27.0 32.275 22.775000000000002 9 17.4 26.424999999999997 32.824999999999996 23.35 10-14 18.836883688368836 31.348134813481348 27.412741274127413 22.402240224022403 15-19 19.98 27.12 28.255000000000003 24.645 20-24 20.28 28.21 27.47 24.04 25-29 20.32 28.48 27.1 24.099999999999998 30-34 20.745 27.435 27.750000000000004 24.07 35-39 20.125 27.750000000000004 28.53 23.595 40-44 20.18 28.03 27.62 24.169999999999998 45-49 20.244999999999997 26.305 27.705000000000002 25.745 50-54 20.565 27.36 27.815 24.26 55-59 20.44 27.04 28.799999999999997 23.72 60-64 21.51 27.27 27.395000000000003 23.825 65-69 19.900000000000002 27.62 27.655 24.825 70-74 20.669999999999998 27.725 27.42 24.185000000000002 75-79 20.505000000000003 27.139999999999997 27.900000000000002 24.455 80-84 20.76 27.694999999999997 27.245 24.3 85-89 20.415 26.640000000000004 28.015 24.93 90-94 20.525 26.69 27.705000000000002 25.080000000000002 95-99 20.77 27.634999999999998 26.919999999999998 24.675 100-104 20.57 27.689999999999998 26.939999999999998 24.8 105-109 21.875 26.729999999999997 27.860000000000003 23.535 110-114 21.115000000000002 28.185 26.895000000000003 23.805 115-119 22.725 27.165 26.83 23.28 120-124 21.52 27.529999999999998 26.63 24.32 125-129 21.335 27.105 27.38 24.18 130-134 21.475 27.744999999999997 26.445 24.335 135-139 21.135 27.810000000000002 26.625 24.43 140-144 21.095 26.205000000000002 27.555000000000003 25.145 145-149 21.87 27.27 26.06 24.8 150-151 21.725 27.6 26.437500000000004 24.2375 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 0.5 21 0.0 22 0.0 23 0.0 24 0.0 25 1.5 26 5.0 27 7.5 28 7.0 29 8.0 30 14.0 31 18.5 32 30.5 33 34.0 34 45.5 35 69.5 36 74.5 37 80.5 38 96.5 39 140.5 40 177.5 41 205.5 42 230.0 43 264.0 44 268.5 45 262.0 46 277.5 47 242.0 48 214.5 49 210.0 50 188.0 51 144.0 52 134.0 53 138.0 54 97.5 55 69.5 56 50.5 57 36.5 58 35.5 59 23.0 60 13.0 61 14.5 62 11.0 63 7.0 64 9.0 65 6.5 66 10.0 67 11.0 68 5.0 69 2.5 70 1.5 71 0.0 72 0.0 73 0.0 74 0.0 75 2.0 76 2.0 77 0.0 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.699999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 61.839863713798984 36.3 2 20.911413969335605 24.55 3 9.327086882453152 16.425 4 4.131175468483816 9.700000000000001 5 2.17206132879046 6.375 6 0.5536626916524702 1.95 7 0.7240204429301533 2.9749999999999996 8 0.21294718909710392 1.0 9 0.08517887563884156 0.44999999999999996 >10 0.04258943781942078 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATAATATTCATAACAATTAGCAACTAGGGTAGGGAACGCCACAATCGC 11 0.27499999999999997 No Hit CTGCCGCCGCCCTTACATGACGGGAAGACACACGACGCCGGCGGTTAGTG 9 0.22499999999999998 No Hit AACCAAAACATTCTCAGAATCATTCAGCTCTCCCTTGGAATGTTCAGTTA 9 0.22499999999999998 No Hit GTTGCTGGGACCACTGAGGGAAGCCATCATTCCCTGCTCTTATTCCACCA 8 0.2 No Hit CCTACACTACAAAACTCCTGCGCACTGGATGGACAGAATGCCACGTCTTC 8 0.2 No Hit TGATCCTCCTTCCCTTCTCACCAAGAACATTTTGGGTACGAGTAGCCCTG 8 0.2 No Hit GTTGGTATTACTGGAGGAGTCACTGGAGGCTTTGGTGGAGGAAGAGTTGG 8 0.2 No Hit GTTGTCGCCTGTTAATAGGATAAAAATATGGAAGTAGCAAGAATCCCTAG 8 0.2 No Hit CACCCTCTATATTGAAGTTGCCGATGGTTTCATAGGCATAAAACCAGCAA 7 0.17500000000000002 No Hit GGACCGGCAATGTAATCATCAAAGTCAGCTAAAAAGGTTAGCTGGAGATG 7 0.17500000000000002 No Hit GGCAGAAAAACTGACAATACACCAGCATTCTTAGCCGCAGCAAGATAATC 7 0.17500000000000002 No Hit ATTTGCCTTTTATACTCATCCGATAATATTTCATATGCTTGGCATATTCG 7 0.17500000000000002 No Hit GGAAGGAAGTGCCAGGGATATTCAAGGGCAAGGAGATGAAACTGTTCAAG 7 0.17500000000000002 No Hit CCTTTATTTCCACCAGCATTACCACCTTTGTTTCCACCAGCATTACCACC 7 0.17500000000000002 No Hit CTCAGCTTTCTTTTGATATAATTCTTCAAGGCATTCTTTAGCTCTATGAA 7 0.17500000000000002 No Hit ACCTTGTACCTGTTCCGGCGGACCTCGGTCCTTGCACTTGGCCTCAACGA 7 0.17500000000000002 No Hit GGCAACCAAACACCAATAGCTTAATTAAAGCATTAAACATCAAGCACCAA 7 0.17500000000000002 No Hit GCCTTGTGAAGAAATGGAAGAAAATGGACCAGAAGATGATGCTGCCAACG 7 0.17500000000000002 No Hit GTTCGAGAGGTCCTTCCTTGAGTTCCACTGTTTCTGTTGCAGTGGTTCCT 7 0.17500000000000002 No Hit CCTGAAATGTTGCAAGGAAGGGTGGCTTCTTCTTGTTTTATTTATGCTGA 7 0.17500000000000002 No Hit GCCTCAGCCAAACTCCCCACCTGACAATGTCTTCCGCCCGGATCGGCCGC 7 0.17500000000000002 No Hit GTATCATGTCCTTGGCTTGCTCAAAGTAACTCTTCTTCTTCTCTGGGGTA 7 0.17500000000000002 No Hit CAGAGTTTAAAATAAACAGTTATGGAGAACTGGTAAAAAATCAAAAAAGG 7 0.17500000000000002 No Hit GTCCCCTTTGGATGATAGCTGTTCATAGACTTCTACCAACAACGGAGTGA 7 0.17500000000000002 No Hit ACGCCTTTGAAAATGAAGAAGAAATATCAGTGTTCCTATACAGGCCATCG 7 0.17500000000000002 No Hit CAATCAAAACCAAACCAATCCAAACCAAACAAGACCTAAGAGACTCACAT 6 0.15 No Hit CCTCTTTTTTCTCCTCAGCAGATTCAGAAGTCTCAATAACAGCAACTGCT 6 0.15 No Hit GTCTCTTGATCGGTGTTTGTGGTGGTCACGATCACGGTCGTCACGGGTGT 6 0.15 No Hit CTACTTACCATCTCCGTGAGCTCCTTTTGGCCCCTAGCAATAGCTTGAGC 6 0.15 No Hit TCCTGTCCTTCAGCTGTGAGCAGCTCATTCATCCTAGAATCAAATTGACG 6 0.15 No Hit AGGTAGGACAGAGCCTCAAACTTTTTGTTGTTGATTGGGTTCCATGTCTT 6 0.15 No Hit GGCCACTAAAAGAAGAACCTCCACAAAAAGATGCCCTCCAATATGAACAC 6 0.15 No Hit ATCCACAGTTTGAACGGTAGGACCAGATGACTGCTCGTGTTCGCGATCAT 6 0.15 No Hit CATCCAATAAGTATGGCCCGGGAGTGTCCAGCATTTTTTGAATTGCTGCT 6 0.15 No Hit CCATAACCTAACTTATAATGTGAATTGTTAAAATTACAAATAGGGTACCG 6 0.15 No Hit CGGTGGATTGATTGCTAGATTCATAATTATCACGATTGGATGAACTAGAA 6 0.15 No Hit TCTTTGTAAGTACTAGCTACCATGGTGGGTCGAATAATGAGTACGGGCAA 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGGATTATCTCGTAT 6 0.15 TruSeq Adapter, Index 25 (97% over 37bp) ACTTCTTTGACTTGCTGGTTGCTAGCTGGGTCTCAGGCTCTTTCTTAACT 5 0.125 No Hit AGCTCATAGACCTCCCCTAGGGCAACTTCACTGAGAAGCATTAGTCCCAC 5 0.125 No Hit CGTTTCTTCACCAAGGTTTATTGGATTACAATTCTTGGTATCCTCCTTTT 5 0.125 No Hit GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA 5 0.125 No Hit GCCCTTGTTAGTCCGCCTTCTTATCTGTTTCATCAATTGCTGCCAGTCTT 5 0.125 No Hit ACCTCAATTAGGTTCACAGTTGTTCGACATCAAAAGCAAACAGTGAATTG 5 0.125 No Hit AACAGAGGAAGCACCAAAAGCCTTGCAACTGTTATGCCAATTGTAGTTCG 5 0.125 No Hit GTAGAAAGGACCTCGACCTCCTCCACACCTAATGCATGGAAGCAGTGCAA 5 0.125 No Hit AGCGTGAATAAGAGACGATTTACTGGTAGATGTGGGTTTTCTGATACCAC 5 0.125 No Hit GACCTTTCCTTAGCATTTGCAAACTCCTCCAATGTTTCTTTAAGTGCTAG 5 0.125 No Hit GGACTCACTGGATAATTCTGTGGAAAGCTCATGGTGGCATTGAAGAACCC 5 0.125 No Hit CAGGGCAATTGTACGTATTTGCGTCGTAGCAGATGCCTGGTACTCCGTTG 5 0.125 No Hit AGGGAAGCGATCATGTATAACAACCACCTCCGGCTACTTTGTCTGCTGCT 5 0.125 No Hit AGAGGAGATTACGACAGTTGGTATTCGACCAAGTCGAATTAGCATGACAG 5 0.125 No Hit GCCTTCACTGCTATTCAGAACAGCAACAGCCTTCACCATTGTTCTCAGAG 5 0.125 No Hit CCTGCAACGATGTCTTTGGTTCAGATGCTTCAGGTTTGTCTTTTCCGTCC 5 0.125 No Hit GTCGGATTCTGTGGCAGTGAAAAGGTGGGTTAGCTTTTCAAAGATGGCCA 5 0.125 No Hit CCTTGATGCTGCTCCAGGACAACAATTCAACATCAAACTGGAGTGTGGCA 5 0.125 No Hit GTGCGAATCAACGGTTCCTCTCGTACTAGGTTGGATTACTATTGCGACAC 5 0.125 No Hit TGGTGAAAGAGCTCGAGAGTGGTGTGACTGTAGGAGACCTAAGAGGGTTT 5 0.125 No Hit GCTATATCTAGAACATTTTTTAGGCATCCATTTAAGCATCCAGAAACACA 5 0.125 No Hit GTTCCATGGACTCGGTGTCTCGCGTGCCAAACTACCGAGAAAGAACCCGA 5 0.125 No Hit AGGGAAACCAACAGCTAGCATGATCAGCTGAATCTTCAATAAAAGGAAGG 5 0.125 No Hit CCACAGAACCCAATTGATTCCACCTGCTTTAAGAAGTAATCCATTCGGCG 5 0.125 No Hit GTGGCCTCTTACACATGGTCCTTTAAAACTATGGCTCTGTGACAAACAAA 5 0.125 No Hit CCTGGATGTAGAAAATCAATAATCAATGAACATCACTTTGATTAGAGAAT 5 0.125 No Hit GTCTGCTCCTGTTTTAACAGCATATCCTTGTGGCACTTTGAACACATGTT 5 0.125 No Hit CCTGCATTGTTCACCAAGATATCAAGCTTTCCAAATTGGCTTTTGATGAA 5 0.125 No Hit CTCCCGGTATCCAAAGAGGTCCACGAGGAGGAGGTATATCCAAAGCAGTA 5 0.125 No Hit CCCTGTACGTGCTCCCCTACAGTAATATTTGACATAGTCCGACCAAAAGT 5 0.125 No Hit CTTCTCTCGGTCAGCTTGAGAGGATACATCACAGACTGATCCGCTAACTT 5 0.125 No Hit CCAACAGCCAACAAAGCCATCCCTAAACCATAGTACCATGCAGCAACATC 5 0.125 No Hit AAGCAGTGGTATGCAACACACCAAGCAGATATGCACATGCGAGTTTCGTG 5 0.125 No Hit GCTCTGAGTAAAGATCGGATAACTTGGGCAGCTTTAAGCCTCTGCTTGAC 5 0.125 No Hit CGGCAGGTTTTGGTTTAGGTTGTCTAGGCTTCTTTTCTCTGGGAGTCCTT 5 0.125 No Hit CCGAGAAGCAAAAAAAATAGCTCTACTATTTCTTAGAGCTGCAAAGCTAA 5 0.125 No Hit CATTATAATGTCCGATGTAGAAATGCCATCAGTTCTTTTCGTTTCCTTGA 5 0.125 No Hit TGCACACACAAGACAACTGAACAGTTTCTGGTATAAATAGGCACAGCTGA 5 0.125 No Hit GCACCATCGACATGCTTATAGATAACAAAACCGTACCCTTTCGACTTTCC 5 0.125 No Hit GTGGGACCATATTATATACCGGCTGCTCTCGGTAAACATCGGGTCCGTGA 5 0.125 No Hit CTTGGAGATCTCTCAAAGGTTCAAATACTAAGTAATTGCCCGAGTCTGCA 5 0.125 No Hit CACTGCAGCAAGGAAGGAAATAGCCAAATGTTTTCCAACATTCCTCTCTC 5 0.125 No Hit CATCCCACACCTGTTTCTTGAGCTGCTGTTGCTTAGCCTTTTTCTCCTGG 5 0.125 No Hit CCCTAATTCATGTAATCAAGCTACTGTATTTACAAACAAGCAAGATGAAA 5 0.125 No Hit ACCCAAGTAGACAGCCAAGAATACAGCTACTTGCTTCAGGCTCTGGTGAC 5 0.125 No Hit GGAACATGAAGAAGAACTTGAATGCCGGTAGCTTTCATGCAGCATAAATA 5 0.125 No Hit CTCTTACACATGGTCCTTTAAAACTATGGCTCTGTGACAAACAAACCCTA 5 0.125 No Hit GTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACGTTC 5 0.125 No Hit CTCAAAGTTCATCTTTAGCTGGCTCTTGTTCTTTAGCTGGCTCTTCTTCT 5 0.125 No Hit CCACTGTTCATTAGGCGCCTGCTTATAGCGGGAGAAATGACTCTTGCTTG 5 0.125 No Hit GTCAATTGCATATATGCGACCAGATATCAATGAAGGAAGGATCAGATAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.0625 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.3125 0.0 0.0 0.0 0.0 82-83 0.35 0.0 0.0 0.0 0.0 84-85 0.4 0.0 0.0 0.0 0.0 86-87 0.475 0.0 0.0 0.0 0.0 88-89 0.65 0.0 0.0 0.0 0.0 90-91 0.7 0.0 0.0 0.0 0.0 92-93 0.725 0.0 0.0 0.0 0.0 94-95 0.825 0.0 0.0 0.0 0.0 96-97 1.0 0.0 0.0 0.0 0.0 98-99 1.1375 0.0 0.0 0.0 0.0 100-101 1.575 0.0 0.0 0.0 0.0 102-103 1.8375 0.0 0.0 0.0 0.0 104-105 2.1625 0.0 0.0 0.0 0.0 106-107 2.55 0.0 0.0 0.0 0.0 108-109 3.0875 0.0 0.0 0.0 0.0 110-111 3.4625 0.0 0.0 0.0 0.0 112-113 3.8 0.0 0.0 0.0 0.0 114-115 4.3875 0.0 0.0 0.0 0.0 116-117 4.9 0.0 0.0 0.0 0.0 118-119 5.275 0.0 0.0 0.0 0.0 120-121 5.625 0.0 0.0 0.0 0.0 122-123 6.175 0.0 0.0 0.0 0.0 124-125 7.0625 0.0 0.0 0.0 0.0 126-127 8.05 0.0 0.0 0.0 0.0 128-129 8.825 0.0 0.0 0.0 0.0 130-131 9.8 0.0 0.0 0.0 0.0 132-133 10.325 0.0 0.0 0.0 0.0 134-135 11.3 0.0 0.0 0.0 0.0 136-137 11.95 0.0 0.0 0.0 0.0 138-139 12.925 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTAACC 10 0.006830828 145.0 7 AAACTAA 10 0.006830828 145.0 5 GGTGAAA 10 0.006830828 145.0 2 TCTAGAT 10 0.006830828 145.0 145 TAAAACT 10 0.006830828 145.0 3 CTAACCT 10 0.006830828 145.0 8 AACTAAC 10 0.006830828 145.0 6 AAAACTA 10 0.006830828 145.0 4 AAAGAGC 10 0.006830828 145.0 6 GAAAGAG 10 0.006830828 145.0 5 GTAAAAC 10 0.006830828 145.0 2 >>END_MODULE SRR26075398 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075398_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.317 37.0 37.0 37.0 37.0 37.0 2 36.3875 37.0 37.0 37.0 37.0 37.0 3 36.4345 37.0 37.0 37.0 37.0 37.0 4 36.4285 37.0 37.0 37.0 37.0 37.0 5 36.365 37.0 37.0 37.0 37.0 37.0 6 36.33 37.0 37.0 37.0 37.0 37.0 7 36.345 37.0 37.0 37.0 37.0 37.0 8 36.3405 37.0 37.0 37.0 37.0 37.0 9 36.354 37.0 37.0 37.0 37.0 37.0 10-14 36.3343 37.0 37.0 37.0 37.0 37.0 15-19 36.3673 37.0 37.0 37.0 37.0 37.0 20-24 36.329 37.0 37.0 37.0 37.0 37.0 25-29 36.2525 37.0 37.0 37.0 37.0 37.0 30-34 36.1161 37.0 37.0 37.0 37.0 37.0 35-39 36.1263 37.0 37.0 37.0 37.0 37.0 40-44 36.1485 37.0 37.0 37.0 37.0 37.0 45-49 36.066500000000005 37.0 37.0 37.0 37.0 37.0 50-54 36.0138 37.0 37.0 37.0 37.0 37.0 55-59 35.988200000000006 37.0 37.0 37.0 37.0 37.0 60-64 36.080200000000005 37.0 37.0 37.0 37.0 37.0 65-69 35.95569999999999 37.0 37.0 37.0 37.0 37.0 70-74 35.9821 37.0 37.0 37.0 37.0 37.0 75-79 35.833800000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.8553 37.0 37.0 37.0 37.0 37.0 85-89 35.8628 37.0 37.0 37.0 37.0 37.0 90-94 35.75760000000001 37.0 37.0 37.0 37.0 37.0 95-99 35.828399999999995 37.0 37.0 37.0 37.0 37.0 100-104 35.66760000000001 37.0 37.0 37.0 37.0 37.0 105-109 35.6269 37.0 37.0 37.0 37.0 37.0 110-114 35.6327 37.0 37.0 37.0 37.0 37.0 115-119 35.6211 37.0 37.0 37.0 37.0 37.0 120-124 35.4485 37.0 37.0 37.0 37.0 37.0 125-129 35.483599999999996 37.0 37.0 37.0 37.0 37.0 130-134 35.367900000000006 37.0 37.0 37.0 34.6 37.0 135-139 35.219300000000004 37.0 37.0 37.0 32.2 37.0 140-144 35.183299999999996 37.0 37.0 37.0 32.2 37.0 145-149 35.129000000000005 37.0 37.0 37.0 27.4 37.0 150-151 34.799499999999995 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 1.0 14 2.0 15 4.0 16 6.0 17 1.0 18 1.0 19 2.0 20 8.0 21 10.0 22 8.0 23 9.0 24 8.0 25 6.0 26 7.0 27 11.0 28 12.0 29 12.0 30 13.0 31 26.0 32 37.0 33 81.0 34 174.0 35 638.0 36 2702.0 37 220.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.425000000000004 22.2 13.750000000000002 28.625 2 28.1 28.549999999999997 26.8 16.55 3 24.525 28.775000000000002 29.125 17.575 4 24.75 34.75 20.849999999999998 19.650000000000002 5 25.324999999999996 36.675000000000004 22.05 15.950000000000001 6 20.525 41.525 21.099999999999998 16.85 7 23.275000000000002 22.650000000000002 35.75 18.325 8 21.5 27.55 25.5 25.45 9 22.0 27.250000000000004 28.675 22.075 10-14 24.03 29.005 25.775 21.19 15-19 24.060000000000002 28.845 26.540000000000003 20.555 20-24 24.295 28.76 26.0 20.945 25-29 24.27 28.34 26.455000000000002 20.935000000000002 30-34 23.985 28.060000000000002 27.205000000000002 20.75 35-39 25.0 28.215 25.974999999999998 20.810000000000002 40-44 25.52 27.779999999999998 26.415 20.285 45-49 24.349999999999998 28.555000000000003 26.3 20.794999999999998 50-54 23.59 27.91 27.089999999999996 21.41 55-59 24.75 28.549999999999997 25.779999999999998 20.919999999999998 60-64 24.595 28.435 26.19 20.78 65-69 25.435000000000002 27.61 26.07 20.885 70-74 24.905 27.525 27.02 20.549999999999997 75-79 24.08 28.939999999999998 27.21 19.77 80-84 24.145 28.884999999999998 25.96 21.01 85-89 25.09 28.235 26.985 19.689999999999998 90-94 25.21 28.07 26.284999999999997 20.435 95-99 24.495 28.415000000000003 26.045 21.044999999999998 100-104 24.9 28.51 25.869999999999997 20.72 105-109 25.155 27.894999999999996 26.619999999999997 20.330000000000002 110-114 25.1 27.800000000000004 26.695 20.405 115-119 25.28 28.084999999999997 25.96 20.674999999999997 120-124 26.06 28.205000000000002 25.814999999999998 19.919999999999998 125-129 26.490000000000002 29.29 25.05 19.17 130-134 27.065 28.405 26.07 18.459999999999997 135-139 27.595 27.985 25.61 18.81 140-144 27.237723772377237 26.57765776577658 27.37273727372737 18.81188118811881 145-149 27.393217965389617 28.258477543262977 25.602680804241274 18.745623687106132 150-151 29.382345586396596 27.956989247311824 24.731182795698924 17.92948237059265 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.5 9 2.5 10 1.0 11 0.5 12 1.0 13 0.5 14 0.5 15 0.5 16 0.0 17 1.0 18 1.5 19 1.5 20 2.0 21 1.5 22 1.0 23 1.0 24 1.0 25 5.0 26 4.5 27 0.0 28 5.0 29 10.5 30 9.5 31 10.0 32 8.5 33 23.5 34 36.0 35 31.5 36 51.0 37 65.0 38 94.0 39 144.0 40 182.5 41 225.5 42 272.0 43 266.5 44 271.5 45 320.5 46 295.0 47 252.5 48 231.0 49 201.0 50 187.0 51 176.5 52 131.5 53 97.5 54 86.0 55 56.0 56 40.0 57 38.0 58 28.0 59 24.0 60 18.5 61 12.0 62 10.5 63 9.5 64 10.5 65 5.5 66 2.5 67 1.0 68 2.5 69 5.0 70 3.5 71 2.5 72 2.0 73 0.5 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.01 145-149 0.03 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.375 #Duplication Level Percentage of deduplicated Percentage of total 1 62.94736842105263 37.375 2 20.210526315789473 24.0 3 8.968421052631578 15.975 4 4.421052631578947 10.5 5 1.8526315789473686 5.5 6 0.5894736842105264 2.1 7 0.631578947368421 2.625 8 0.25263157894736843 1.2 9 0.08421052631578947 0.44999999999999996 >10 0.042105263157894736 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTCTACACCACCCTCCACCACTTGCTGCAGCAAATTGAAGGAGCAGAAG 11 0.27499999999999997 No Hit CTTTCCTAGTGCCCCTCCTCCTTCTTCAGCACTACGGCTACAGATTATTC 9 0.22499999999999998 No Hit TAGAAATACAGAGTTTCAGTCTTTCAGAATTCAAGAATGGCTTCCACTTC 9 0.22499999999999998 No Hit AAGCCACCAAAGCCTCCTATTAAGCCACCAAAGCCTCCTGTGACTCCTCC 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 8 0.2 No Hit AGATGATTTTGTGTTTTATGAGGATCGGGAGAAGATGATCCAGGAGAAAA 8 0.2 No Hit AGCTCAGCATGCTTTTGCAATGATGGATAATAATGCTAGTGCTCCGCAAA 8 0.2 No Hit CAAAATCTGGTGTTGCCCACGAAACAGTGCAGAATATTAGAAGAGGAAGT 8 0.2 No Hit CCAGGACCATATAACATCATCTGCCTCTGATCCAGCCACTAAGTCTCCAG 8 0.2 No Hit GAAATACCACTACTTTTAACGTTATTTTACTTATTCCGTGAATCGGAGGC 7 0.17500000000000002 No Hit CCGGAAGCCACCGATGAAGATATCAGAAAGGCTTATCGACATTGGGCTCA 7 0.17500000000000002 No Hit AGAACGTTATGGCTATTCTCGAGCATGCTATGCTCTGTCTCTTATCTCCC 7 0.17500000000000002 No Hit GATCAGGTTAAGGTCAGCAATTTGGTTGGGAAGATTGTGGGATTCTACTT 7 0.17500000000000002 No Hit AATGGTGCAGTTCCTTACAAACCTAAGTATCAAAATTCATATGGAAGGGG 7 0.17500000000000002 No Hit GAAGATCTTAAAGGCCCCAAAGTTTGACCTTGGAAAATTGATGGAGGTTC 7 0.17500000000000002 No Hit CATAACTTTGAACCACTTTGGCGTTGAGAGCCTGAAGGAATCATTGCTTC 7 0.17500000000000002 No Hit GAGGAAAGGGTGGCAACAACGGTGGCAACCAAGGCGGAAACGGTGGAAAG 7 0.17500000000000002 No Hit GAGAAAATTCATCGATCAGACAACAGTTATCCTGGTGACGGGGAGAAGAA 7 0.17500000000000002 No Hit AGATTCTTCCTTGGATCAAACAAAGTAATGCAGGTTTCTCTGGGTCGATC 7 0.17500000000000002 No Hit CGGATCTTTGCCCTTTGACGTGTAAATCTGCAACTTTTGGCAACTGTACA 7 0.17500000000000002 No Hit ACTGGAAGTGGTGATTATAATTGTGACTCTGTGGACCCTTGGATGCTTGA 7 0.17500000000000002 No Hit GATGGTTACTCTGGTGTTGAAGTTAGGGTTACTCCCATGCGCACTGAGAT 7 0.17500000000000002 No Hit ACGGCGGCAAGCACGGCGGTGGAAAGTTCAAGCGTGGGAAGTTTGGGAAG 7 0.17500000000000002 No Hit CTTCTTAGGAGCTCATCAACGCCAATTCTGAATTCATGGATCCCTCCACA 7 0.17500000000000002 No Hit CAAATACAATAATCCTAGCCGTTCCATCACTACCTTCACGAAAATCTTAA 6 0.15 No Hit ATTTAAGAAAGCCTTGCAACTGGACCCCAGTAATGAAACGGTCAAAGAAA 6 0.15 No Hit GCCTCAATCTATCAAGAGAAACGAACAGGGGATAACAGCTATCCACAAAT 6 0.15 No Hit CAAAAAGATCCGAGGAGTTTGTGGATTCTCAATCAAATCTTCTATCTCTT 6 0.15 No Hit GTTTAAGAAGGAATAGAGAAATGTGGTCAGCTATGGGTACTCTTGAAAAC 6 0.15 No Hit CCACTTCTACGACAACCTCTCTTTGCTGTTCTCCCTTAGCAAACAGTTTA 6 0.15 No Hit GCTCTGAGCTTCAGAATTACTTTACCCGTATCCTAGAGGATAATTTGAAG 6 0.15 No Hit CAATGAGAACCGCAGTCGCCATTGTCCTGCTGGGCAGTCTGTGCATTTGT 6 0.15 No Hit CTATTCGTGTGGAGAATCTCCCTATCAAGATAATGCTTCTGAACAATCAG 6 0.15 No Hit TTCAGCTTTTATATCCCCTATTCTCTTCGTGAAGATGTCTTGCTGTGGAG 6 0.15 No Hit CGGGTTTGGTTCTGGCTATTTGAGCGGACTTTGTTCAGGTGCATCCGGGA 6 0.15 No Hit AGAAGGTGGAAACTGAAACCCCATCAGAGACAACTCCTCCACCACCAGCT 6 0.15 No Hit AATCAAACAAAATCCTCAATTAATTGTTCGATTGATTGCTTAATCCTTCA 6 0.15 No Hit GAAGCGTACCTCTTGCCGACGATTTCTCCTCGTAATGTTAAGAAAATAGA 6 0.15 No Hit GGGTAAAAGCTCGTTTGATTCTGATTTCCAGTACGAATACGAACCGTGAA 5 0.125 No Hit AACAGAAACAGAAAGTCGAAATTTGAAATTTTGGGAGAGAGATGGCAACA 5 0.125 No Hit AGATGAGTATGCTGATATCTATATCACCACCAGCTGCACAGTCTTTCTTT 5 0.125 No Hit ACGACAAACAAGCATACTCTCTCTTTCTCTCTCTACTCTCGAAGGGGCGC 5 0.125 No Hit AGGAGGAGCAAGCTACCATGTGGAACTTAAGTATTGCACTTGAGCCATTG 5 0.125 No Hit CTCTAAGCTCCAGCTCAGGGGAGGTCCCATCCCATGGAGTCCTTCTCTTT 5 0.125 No Hit CCCCAGTCCTAATGCCTAAAACTCAGCTAACACAAAGAAGGACACAACCC 5 0.125 No Hit CAGTGATTGTTATTATGGTGGTTGTGCTGAGGCGCCCTTAAAAGTTCATT 5 0.125 No Hit AAGGGCCTTACTCCACTTCACTATGCTGTTCAAGGGTCCCATCTGGAGCT 5 0.125 No Hit AGGATGGGACCTAGGTATCAAGACAATGAAGAAAGGTGAAAATGCCCTTT 5 0.125 No Hit AGCACCTCCTGCGAATTTGCCTGTGACAATGCCACAACAGGTGACCGCTT 5 0.125 No Hit GTTACAAAGTCGCCCAGTTGAGTTAGATGGGTTTGATGCAGCACCGATAA 5 0.125 No Hit TATGCCAAACTTAGGAGATGAACTACATCATTCAGGGTGGAATTCTTGTA 5 0.125 No Hit GATGAAGAAGGTGCAAGGACTGCATTAAGTTTGTCAGGGACTGTTCTTGG 5 0.125 No Hit AACACTACCACCATCGTCTGATCGCCCTGTTCGTGTTTATGCTGATGGGA 5 0.125 No Hit GAACCAATGTGGATCTGGCGAAACTCCTCTCTGCTGGGGATTTCATTTGC 5 0.125 No Hit AGGTGACAATGGAACTATTGATGGCCAAGGCAGCATATGGTGGGACTGGT 5 0.125 No Hit AAATCTCCCCGCTTCTCTAGAAACTCCCAATTTCCATTTCCACCAAAATG 5 0.125 No Hit GTTATAGAGAAACGCTTAAGAACAGAATGCTGCTATGGCATGGTTCTCGG 5 0.125 No Hit GACAGGTTCTTGTTAGACAGAGAGATGGAGAAGAAATGCTATGGTCTTTT 5 0.125 No Hit GGGTTATATCCAAAATCAAAACCAGATTCAGGCTCATCACCACATCAGAA 5 0.125 No Hit TGTGAAACTACAGGAAGAAAATTTGTCATCTATGAATACGGATCCTTGGT 5 0.125 No Hit TGCTATAGTAATTGACATGGCCAATGAAGTCTTACCAGTTGTCAAGGAGG 5 0.125 No Hit GCTTTCTCCACTTGATCTATCTATGGGGAAAGAGGATGGCAACTCAAGCT 5 0.125 No Hit GTTTGAGGACACCAACTTGTGTGCTATTCATGCCAAGAGAGTCACCATCA 5 0.125 No Hit GTCCATTCAAGGGACAGTTTTAATTATCACGGTGTCCTGAGTTATGGTGT 5 0.125 No Hit ATCAGGGGCATCTTGTTGATCTTGTTCAAAGAGAGGTGAAAGCATTGCTG 5 0.125 No Hit GCAAGACATCATTGATTTCATTGAGAAGAACAGGGATAAAATTGGCCAAC 5 0.125 No Hit TAAAAATATAGAGGGAGCACATAACAAGCACGATAGGGAGTGTGTGAGAG 5 0.125 No Hit CGCCAAGCCTTGTTAGTGAAATAATGCAACTCGCGCAAAAGAAAAATGCA 5 0.125 No Hit CTTCTATCCAAAGCCTTGCAGACAAATCAATTACCCGTTTCTATTTTGAT 5 0.125 No Hit CCAGAGGGTGCAATGTGCTCAGCAATAATCAATGAGCAGGGGATGATTGT 5 0.125 No Hit CCTTGAAGCCGTCCGATCCATCGCAGACCCCGACGCCACGCAACGGAAGC 5 0.125 No Hit CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATA 5 0.125 No Hit AGAAGCTTGAAATCGATGATGACCAGAAATTGCGGGCATTCTTTGACAAG 5 0.125 No Hit GCAAAAAAATGCACTGGAACATGTTTTTTGGGGTCTGATTCACACAGCAC 5 0.125 No Hit CCCTCGCCAAGCAGAAAAAGGGCGCCGCTCACTGGAAGCCGGAGCTCGCG 5 0.125 No Hit GAATTTCTGTATCTCCCAAGTAGGCAGTGAAGATGTCTCATCCAGAAATC 5 0.125 No Hit TCAAGGGTTTGGATGTGGATGCACTCTACATTACTCACATCCAGGTGAAT 5 0.125 No Hit GCAAAAAATGGACCGTGACGAGACAGGATGCCAAGCTCCTCCAGAACGTC 5 0.125 No Hit AAAAGATGGAAGTTAAGAGACAAAGCGAGCAGCTTCTTCCTCCAAGCCCT 5 0.125 No Hit GTCTCTTCAATCCTTTTGTTGTGTGTTTTCTACTCTGCCTGATCACCATG 5 0.125 No Hit GTCCGTGGTGAAGGAGGAGAGAAATAGAAGAAGAAGAATGATGATGGCCA 5 0.125 No Hit GGGTGATGTCTTTATGGGTAGCTTCCACACAGTATTCGATTATGGCAATA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.0625 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.3125 0.0 0.0 0.0 0.0 82-83 0.35 0.0 0.0 0.0 0.0 84-85 0.4 0.0 0.0 0.0 0.0 86-87 0.475 0.0 0.0 0.0 0.0 88-89 0.65 0.0 0.0 0.0 0.0 90-91 0.7 0.0 0.0 0.0 0.0 92-93 0.725 0.0 0.0 0.0 0.0 94-95 0.825 0.0 0.0 0.0 0.0 96-97 1.0 0.0 0.0 0.0 0.0 98-99 1.1375 0.0 0.0 0.0 0.0 100-101 1.625 0.0 0.0 0.0 0.0 102-103 1.9 0.0 0.0 0.0 0.0 104-105 2.2625 0.0 0.0 0.0 0.0 106-107 2.675 0.0 0.0 0.0 0.0 108-109 3.2125 0.0 0.0 0.0 0.0 110-111 3.5875 0.0 0.0 0.0 0.0 112-113 3.9250000000000003 0.0 0.0 0.0 0.0 114-115 4.525 0.0 0.0 0.0 0.0 116-117 5.0625 0.0 0.0 0.0 0.0 118-119 5.45 0.0 0.0 0.0 0.0 120-121 5.85 0.0 0.0 0.0 0.0 122-123 6.4 0.0 0.0 0.0 0.0 124-125 7.262499999999999 0.0 0.0 0.0 0.0 126-127 8.225000000000001 0.0 0.0 0.0 0.0 128-129 9.05 0.0 0.0 0.0 0.0 130-131 10.0 0.0 0.0 0.0 0.0 132-133 10.537500000000001 0.0 0.0 0.0 0.0 134-135 11.5625 0.0 0.0 0.0 0.0 136-137 12.2 0.0 0.0 0.0 0.0 138-139 13.1625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGCT 10 0.006830828 145.0 7 GCGACGT 10 0.006830828 145.0 1 ACGTTGC 10 0.006830828 145.0 4 TATGCTG 10 0.006830828 145.0 8 AGTATGC 10 0.006830828 145.0 6 AGATGAG 10 0.006830828 145.0 1 CGACGTT 10 0.006830828 145.0 2 CGTTGCT 10 0.006830828 145.0 5 GCTTTTT 10 0.006830828 145.0 9 TGAGTAT 10 0.006830828 145.0 4 GTTGCTT 10 0.006830828 145.0 6 >>END_MODULE Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783719 spots for SRR26075398.sra Written 1783719 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra Read 1783706 spots for SRR26075398.sra Written 1783706 spots for SRR26075398.sra SRR ids: ['SRR26075398.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_gx1qnjwx SRR26075398.sra spots: 35674133 blocks: [[1, 1783706], [1783707, 3567412], [3567413, 5351118], [5351119, 7134824], [7134825, 8918530], [8918531, 10702236], [10702237, 12485942], [12485943, 14269648], [14269649, 16053354], [16053355, 17837060], [17837061, 19620766], [19620767, 21404472], [21404473, 23188178], [23188179, 24971884], [24971885, 26755590], [26755591, 28539296], [28539297, 30323002], [30323003, 32106708], [32106709, 33890414], [33890415, 35674133]] SRR26075398 file size 13174140 SRR26075398 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075398 SRR26075398_1.fastq SRR26075398_2.fastq Input file: SRR26075398_1.fastq Paired file: SRR26075398_2.fastq trimmed: SRR26075398-trimmed-pair1.fastq, SRR26075398-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:01:39 2025 >> started Wed Feb 12 01:02:21 2025 >> done (41.893s) 35674133 read pairs processed; of these: 140 ( 0.00%) short read pairs filtered out after trimming by size control 75101 ( 0.21%) empty read pairs filtered out after trimming by size control 35598892 (99.79%) read pairs available; of these: 6537540 (18.36%) trimmed read pairs available after processing 29061352 (81.64%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 16 0.00% 19 9 0.00% 20 15 0.00% 21 14 0.00% 22 19 0.00% 23 21 0.00% 24 23 0.00% 25 26 0.00% 26 24 0.00% 27 38 0.00% 28 44 0.00% 29 43 0.00% 30 37 0.00% 31 57 0.00% 32 50 0.00% 33 52 0.00% 34 75 0.00% 35 45 0.00% 36 45 0.00% 37 66 0.00% 38 81 0.00% 39 60 0.00% 40 104 0.00% 41 78 0.00% 42 87 0.00% 43 102 0.00% 44 118 0.00% 45 137 0.00% 46 132 0.00% 47 164 0.00% 48 206 0.00% 49 262 0.00% 50 224 0.00% 51 234 0.00% 52 304 0.00% 53 286 0.00% 54 300 0.00% 55 397 0.00% 56 406 0.00% 57 531 0.00% 58 527 0.00% 59 608 0.00% 60 697 0.00% 61 784 0.00% 62 985 0.00% 63 1032 0.00% 64 1170 0.00% 65 1316 0.00% 66 1487 0.00% 67 1773 0.00% 68 1969 0.01% 69 2164 0.01% 70 2488 0.01% 71 2901 0.01% 72 3205 0.01% 73 3841 0.01% 74 4445 0.01% 75 4727 0.01% 76 5631 0.02% 77 5965 0.02% 78 6893 0.02% 79 7511 0.02% 80 8827 0.02% 81 9715 0.03% 82 11237 0.03% 83 12469 0.04% 84 13989 0.04% 85 16283 0.05% 86 17798 0.05% 87 18469 0.05% 88 21026 0.06% 89 21803 0.06% 90 23447 0.07% 91 26127 0.07% 92 28562 0.08% 93 31185 0.09% 94 34163 0.10% 95 37817 0.11% 96 39327 0.11% 97 43716 0.12% 98 45573 0.13% 99 47085 0.13% 100 49778 0.14% 101 50958 0.14% 102 54775 0.15% 103 57905 0.16% 104 61770 0.17% 105 65334 0.18% 106 68836 0.19% 107 72211 0.20% 108 75100 0.21% 109 77092 0.22% 110 78644 0.22% 111 81328 0.23% 112 83313 0.23% 113 85632 0.24% 114 89332 0.25% 115 91774 0.26% 116 97299 0.27% 117 99356 0.28% 118 102980 0.29% 119 105051 0.30% 120 107044 0.30% 121 108822 0.31% 122 110725 0.31% 123 113985 0.32% 124 116862 0.33% 125 119200 0.33% 126 123390 0.35% 127 126370 0.35% 128 129784 0.36% 129 132465 0.37% 130 135074 0.38% 131 136091 0.38% 132 136157 0.38% 133 139293 0.39% 134 140683 0.40% 135 142149 0.40% 136 145671 0.41% 137 147365 0.41% 138 151726 0.43% 139 153756 0.43% 140 154598 0.43% 141 158740 0.45% 142 159161 0.45% 143 158077 0.44% 144 162427 0.46% 145 163464 0.46% 146 164248 0.46% 147 166138 0.47% 148 169179 0.48% 149 169086 0.47% 150 171673 0.48% 151 29061352 81.64% 35598892 reads passed initial QC criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=4.60 fanout-score-rank=20 prefix-density=0.32 prefix-fanout=3.4 sequence=TCCACACTTGCAGCCATTCTC criterion=fanout-score sequence-density=0.06 sequence-density-rank=31 fanout-score=373.44 fanout-score-rank=1 prefix-density=0.76 prefix-fanout=27.5 sequence=TCATCATCACCACCATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTC criterion=sequence-density sequence-density=0.43 sequence-density-rank=1 fanout-score=2.56 fanout-score-rank=26 prefix-density=0.44 prefix-fanout=2.5 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=104.75 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=5.4 sequence=CAAAGCAGTTGCATTTATCTAAAGTATTCTCACTTTACTTAACACTTGAGCTACATAGAATGTCTACAGTCAATTTGGCAGCACTGCTGTTGCTTGGGCTGCTGCTGGTTATGCCACAGCAGTCCATGCAAGCGAGTTTGATAGACCCCATTGCTGAAATCGAGAGAAGCAACTGCAAAATCGCACACCTTCGCTTAGGGCTTGTTTTTACGTCTGATAACAACGAAAGGGCTCTGCAAGACTCTGGACTCTATAGCCCTGACAGTGAAGACTCTTCTGTTGACATTGCGGGCAGAAGGTTCCATAGCGGGACACTAAACGGTTCTTCTATTGTATATGTCAAGACAGGGAGTCATTCAGTAAATATGGCAACAACTTTGCAAATCCTT SRR26075398 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:03:00 Started mapping on | Feb 12 01:03:00 Finished on | Feb 12 01:07:48 Mapping speed, Million of reads per hour | 444.99 Number of input reads | 35598892 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 32758012 Uniquely mapped reads % | 92.02% Average mapped length | 291.46 Number of splices: Total | 31276327 Number of splices: Annotated (sjdb) | 30498370 Number of splices: GT/AG | 30694458 Number of splices: GC/AG | 447751 Number of splices: AT/AC | 32975 Number of splices: Non-canonical | 101143 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 3.04 Insertion rate per base | 0.02% Insertion average length | 2.55 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 945899 % of reads mapped to multiple loci | 2.66% Number of reads mapped to too many loci | 424842 % of reads mapped to too many loci | 1.19% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.80% % of reads unmapped: other | 0.33% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1894981 1894981 1894981 N_multimapping 945899 945899 945899 N_noFeature 925739 32396059 1117282 N_ambiguous 340898 2121 169230 UnstrandedReadsAssigned:31491375 PositiveStrandReadsAssigned:359832 NegativeStrandReadsAssigned:31471500 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075398 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075398-trimmed-pair1.fastq SRR26075398-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 35,598,892 reads, 32,019,708 reads pseudoaligned [quant] estimated average fragment length: 208.09 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,307 rounds 52401 SRR26075398.ke.tsv 34699 SRR26075398.se.tsv 87100 total ==> SRR26075398.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1810.91 3429 49.0776 Potri.005G024800.1.v4.1 1035 827.91 3234 101.244 Potri.004G059700.1.v4.1 961 753.91 9 0.309411 Potri.007G009000.2.v4.1 1416 1208.91 0 0 Potri.003G141000.2.v4.1 2943 2735.91 1683.37 15.9474 Potri.016G087400.1.v4.1 270 93.5253 2615 724.695 Potri.015G069301.1.v4.1 564 358.677 0 0 Potri.010G195200.1.v4.1 1773 1565.91 773 12.7946 Potri.012G127500.1.v4.1 977 769.91 36801 1238.89 ==> SRR26075398.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 33 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 674 Potri.001G212900.v4.1 2 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1497 SRR26075398 completed mapping pipeline successfully