Starting /dee2/code/volunteer_pipeline.sh SRR26075399 current disk space = 3051218481152 free memory = 1471060180 SRR26075399 SRAfilesize 7f4de587c3d3b63b4f0a7f2588ef4446 SRR26075399.sra SRR26075399.sra file validated SRR26075399 is paired end SRR26075399 is conventional basespace SRR26075399 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075399_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6665 37.0 37.0 37.0 37.0 37.0 2 36.5545 37.0 37.0 37.0 37.0 37.0 3 36.625 37.0 37.0 37.0 37.0 37.0 4 36.6585 37.0 37.0 37.0 37.0 37.0 5 36.716 37.0 37.0 37.0 37.0 37.0 6 36.6585 37.0 37.0 37.0 37.0 37.0 7 36.67 37.0 37.0 37.0 37.0 37.0 8 36.5915 37.0 37.0 37.0 37.0 37.0 9 36.681 37.0 37.0 37.0 37.0 37.0 10-14 36.6579 37.0 37.0 37.0 37.0 37.0 15-19 36.6082 37.0 37.0 37.0 37.0 37.0 20-24 36.5732 37.0 37.0 37.0 37.0 37.0 25-29 36.5501 37.0 37.0 37.0 37.0 37.0 30-34 36.4739 37.0 37.0 37.0 37.0 37.0 35-39 36.416999999999994 37.0 37.0 37.0 37.0 37.0 40-44 36.40859999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.358799999999995 37.0 37.0 37.0 37.0 37.0 50-54 36.291700000000006 37.0 37.0 37.0 37.0 37.0 55-59 36.2787 37.0 37.0 37.0 37.0 37.0 60-64 36.224000000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.159200000000006 37.0 37.0 37.0 37.0 37.0 70-74 36.1811 37.0 37.0 37.0 37.0 37.0 75-79 36.0912 37.0 37.0 37.0 37.0 37.0 80-84 36.091899999999995 37.0 37.0 37.0 37.0 37.0 85-89 36.0048 37.0 37.0 37.0 37.0 37.0 90-94 35.9782 37.0 37.0 37.0 37.0 37.0 95-99 35.979200000000006 37.0 37.0 37.0 37.0 37.0 100-104 35.91799999999999 37.0 37.0 37.0 37.0 37.0 105-109 35.878 37.0 37.0 37.0 37.0 37.0 110-114 35.7217 37.0 37.0 37.0 37.0 37.0 115-119 35.679500000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.7145 37.0 37.0 37.0 37.0 37.0 125-129 35.564499999999995 37.0 37.0 37.0 37.0 37.0 130-134 35.3248 37.0 37.0 37.0 34.6 37.0 135-139 35.2786 37.0 37.0 37.0 32.2 37.0 140-144 35.1734 37.0 37.0 37.0 29.8 37.0 145-149 35.0681 37.0 37.0 37.0 27.4 37.0 150-151 34.872749999999996 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 1.0 22 0.0 23 3.0 24 1.0 25 6.0 26 9.0 27 11.0 28 26.0 29 24.0 30 45.0 31 32.0 32 55.0 33 83.0 34 124.0 35 437.0 36 2927.0 37 215.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.568284142071036 15.632816408204103 8.629314657328663 39.1695847923962 2 19.2 14.025000000000002 34.275 32.5 3 18.15 13.925 29.4 38.525 4 23.799999999999997 21.725 24.825 29.65 5 24.825 26.775 24.45 23.95 6 22.3 31.724999999999998 22.375 23.599999999999998 7 16.775000000000002 28.15 39.35 15.725 8 18.8 26.5 32.05 22.650000000000002 9 18.6 22.875 34.125 24.4 10-14 20.175 29.5 27.445000000000004 22.88 15-19 20.225 27.58 28.345 23.849999999999998 20-24 20.9 28.110000000000003 27.41 23.580000000000002 25-29 20.16 28.244999999999997 27.325 24.27 30-34 20.405 27.205000000000002 27.950000000000003 24.44 35-39 19.759999999999998 27.465 28.53 24.245 40-44 19.869999999999997 27.625 28.134999999999998 24.37 45-49 19.794999999999998 28.225 27.79 24.19 50-54 20.810000000000002 27.74 27.18 24.27 55-59 19.825 27.57 28.105000000000004 24.5 60-64 20.32 28.075 27.700000000000003 23.905 65-69 20.145 27.565 27.694999999999997 24.595 70-74 19.895 27.425 27.894999999999996 24.785 75-79 20.09 28.000000000000004 27.485 24.425 80-84 21.215 27.72 27.49 23.575 85-89 21.17 26.889999999999997 28.005000000000003 23.935000000000002 90-94 20.375 26.685 28.18 24.759999999999998 95-99 20.465 27.71 28.095 23.73 100-104 21.065 27.68 27.215 24.04 105-109 20.9 27.560000000000002 27.0 24.54 110-114 21.755 27.794999999999998 26.845000000000002 23.605 115-119 21.26 27.985 27.565 23.189999999999998 120-124 21.41 28.205000000000002 26.240000000000002 24.145 125-129 21.759999999999998 27.46 26.450000000000003 24.33 130-134 21.23 26.915 26.655 25.2 135-139 22.555 28.22 25.935000000000002 23.29 140-144 21.02 28.57 26.455000000000002 23.955000000000002 145-149 21.625 27.250000000000004 26.52 24.605 150-151 22.3625 27.8375 25.2375 24.5625 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.0 23 0.5 24 2.5 25 2.0 26 5.0 27 10.0 28 12.0 29 12.5 30 12.5 31 12.5 32 23.5 33 34.0 34 38.0 35 57.5 36 80.0 37 102.5 38 117.5 39 134.5 40 181.5 41 220.0 42 211.5 43 234.0 44 278.0 45 287.5 46 276.5 47 258.5 48 219.5 49 187.0 50 175.0 51 147.0 52 126.5 53 117.0 54 111.5 55 83.5 56 42.5 57 34.5 58 31.5 59 21.5 60 18.0 61 18.0 62 16.0 63 8.0 64 5.0 65 2.5 66 3.0 67 6.5 68 7.0 69 3.5 70 1.0 71 2.0 72 4.0 73 2.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.025 #Duplication Level Percentage of deduplicated Percentage of total 1 62.10569438754609 37.9 2 22.695616550594018 27.700000000000003 3 9.012699713232282 16.5 4 3.68701351904957 9.0 5 1.5157722244981564 4.625 6 0.4916018025399427 1.7999999999999998 7 0.24580090126997134 1.05 8 0.12290045063498567 0.6 9 0.0 0.0 >10 0.12290045063498567 0.8250000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CACTAAGGTTCACATTACTACTGAATTCATGTAATAATTTAACAAAGACC 11 0.27499999999999997 No Hit GTTTTTTTGTGGTCTTTTTCTGGATGAGTTTCAGCTAGAATTGAAGAACA 11 0.27499999999999997 No Hit TATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTA 11 0.27499999999999997 No Hit CTTTGGCTTTGGCTTTGGTTTTGGTTTTGGAGACGGGCAACCCCCTCCAC 8 0.2 No Hit CCCAAGAAAAGCAGAGGAGAGGAGCGGCATAGTCCATGTCCAAGAAGTGA 8 0.2 No Hit GTCCCTGAAGCACAAACTTTGTTAATGGTCGTGCAAACTACTGAATTAGA 8 0.2 No Hit GCTTGATTCAAACTGGTTGCCTGTGTGCTTGGAGTTGGATCCGGCACACG 7 0.17500000000000002 No Hit ATGGCTTTTAATGACATCGAGACCCATTTCAGTTGGATCGGTTGCCTGCA 7 0.17500000000000002 No Hit CTGGAGTTCAACTTCACCAGTCTCCACATGCTTCAGTCTAAGAAACAGGT 7 0.17500000000000002 No Hit AACTAGTCTTTTTTAGCTTGTCAAAAACAATGGAAGCACTCAATGTAGAA 7 0.17500000000000002 No Hit ATTGACTCAGGAAGATTCTTTTCTAGAATACTCCACTCCTGTTGAACCTT 7 0.17500000000000002 No Hit GTTTCGAGAGAGATCGATCAAAGAACTGAGAAGGGTTGCCGGGGAGATTC 7 0.17500000000000002 No Hit GATTGAGATACTGTCATATCTTGAGTTTGAAGACCGACTTCTAACTTTAG 6 0.15 No Hit ACACGTTGTCTAGGATTCTGTTTGATCCGTGAGAAAATAATATTTACAAG 6 0.15 No Hit TGCACGGAAGACGTTTGGGCCCACGCCACGAGCGAGCGAGGATACGCCTT 6 0.15 No Hit GAGGTTTCTTCTTCCAGGTGACAATTGCACATGTATAAAAAGATGTACAA 6 0.15 No Hit CATCCATATGCCAACCAAAGCCTAGAAAAACAGCTGCACCTCTCTTCTAC 6 0.15 No Hit ACCTCGGAGACGGAGGACAAGGTGAAGGGTGGACTCCTTCTGGATATTGT 6 0.15 No Hit GTGGGATGTTAAGGTTGATACCCAGGATGTTAGCTTTGATGGCAGTGCAA 6 0.15 No Hit CCCTCCTTTGCCACCAAGAACTAGCGACAGTTCCGGGTCAACGGAGAGAG 6 0.15 No Hit GTGAAACGTAACCTGCTTTGCCTTCACCTGCCTGTTCTTCACATCCACCT 6 0.15 No Hit CCTACCAGCTCTGTGTCGAATCACAAAATCGTACGCTGACATGTCTAGCG 6 0.15 No Hit CACCTCATTGTCCTTTGACCAGTCAACATGATCACTGATAAATCACCAGC 6 0.15 No Hit GCTCCAGCCTGGCAAGCAGTCACAGTGCTACAACCGCATGCCTGTCCGTT 6 0.15 No Hit GTGCTTGTGAATGCTTTGGAAATATCTCTGTTTCTCTAATAGCTTCTCAC 5 0.125 No Hit CTATGATTTGATGAAGTAAAGGGGAGTAAAGGGGTTTGGTTATATAGGTG 5 0.125 No Hit GGGTGAACCTTCGCGGAGAGGGTCGATGAAGATGATGGTGCGAATTGGAG 5 0.125 No Hit GTCGAAAACAAGAGGGTTGGGGGTCCAGGGTCCCTCAAATCCAGAACGCT 5 0.125 No Hit CCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCC 5 0.125 No Hit GTTGCTAGAGAGCTTAGTGCTTGCAGGTTGCCTTCCGAGCTCATACTTTC 5 0.125 No Hit GCCCCATCTTCTTCAGCCTCGTCTTCTGGGTTCATTTCCAAAATCTCGAC 5 0.125 No Hit AGAGTGCAGTATTTGCCCGTTTATGACCTGATAGACACCTAAACAATAAT 5 0.125 No Hit CAAGGCTATAGACTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAG 5 0.125 No Hit GTTGCAAATAGGCTCATTGGGGCATTCACGTGCAAGGTGACCTGTTTTCC 5 0.125 No Hit GGCCGCTCTATGAGCATGAGGTCTTGCGAGCCCCCACTTTCATCCGTAGA 5 0.125 No Hit CCGTGAGTCACGATTGATCGGTTTTACACAGCTGCCATACATTTGTGCAT 5 0.125 No Hit ATTTATACATTGCCTCCATAACCTCAGCCCATGGCATACTTCTGTGTCCA 5 0.125 No Hit GATGCATGCAAGACAGATATGGTGACCAATTAAGCACCAGCTATCTAGAT 5 0.125 No Hit CCCAGTCAAGAAGACAAACATTTTCGTTCTCTGGCCTGTAACTTGAGTTG 5 0.125 No Hit GCTTCAGCAAGTAACTTTTCATCCAAAACCAAATCTTCTGGAACCTCGGT 5 0.125 No Hit GGTGTCAGCAAGGAAGGCGTTGTAGGAGGCTAAATAGCTAACTCCTTTGT 5 0.125 No Hit CTTGAATCGGCACTCAGATTCCAACATCACCCGACAATCCCAGTTTGACC 5 0.125 No Hit CCCGCCATTCTTCTGGTCACTAAAACATCTTCTCCACTCTTTATATGCTT 5 0.125 No Hit AGCTAATTTATGCGCTGGAACTTCTAAACCCACGGAATTTGCATTGCCTG 5 0.125 No Hit CACCCCTGCTATAAGACTTGTTGGGTCGCCCAGCACCGCCGATATAACAA 5 0.125 No Hit TGGGTATTTCTCCCTGATTCTCGCAGCTTCAGCTCTCCTCTTCTCAAAAT 5 0.125 No Hit CGTCAAAGGTGGATTGATCGCTGTCACTTGAGATGAAGATCACCTCAAAT 5 0.125 No Hit CCCTTCTAAGGACCTCTCCAGGCCTTCTGTCAGTAATTCTGTTTCCATCA 5 0.125 No Hit CCGTCGTTGCTGGCACATCGCACAAATGGTGGCCGGCTTAATATTTGCAA 5 0.125 No Hit CATGAGTGTTTAATGGTGTGATGGATCTTGATGCTGCAGGTCCAAGTTTA 5 0.125 No Hit TCTGTGAGTGATTTTCCAAGCGAATATTTTGCATCGAGAATTTGTGTGAG 5 0.125 No Hit CTTGCAGAGCGCGAACCTCGGTCCCCCCAAGGGTATTGTGCAGAGGGCTA 5 0.125 No Hit CTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGA 5 0.125 No Hit AGTCCCTAGGCTCCACCCCAGTATCATCGACTTCTTCCTCTTCTTCCTCT 5 0.125 No Hit GTCCTTCTCATCAAGCACTCCTCTTCTCCTAGTCCTTCACAACTCTCAAC 5 0.125 No Hit CTTGCATCCGCAACAGCCTCGGTGAGACTGTTGAGTTTGATATTGGCCTG 5 0.125 No Hit TGCTTGTCTTCTCGAAGATCAAGTTTTGTTCCAACGAGAATAATTGGAAC 5 0.125 No Hit GGCTTCCACACAACAAAATATATGCAATCACACCAATACTCCACACATCA 5 0.125 No Hit CATCAGACTCCGTTTTAGCTGTCTCGCTCACAACCCTCCAGTAGCTCCTC 5 0.125 No Hit ATTGGGCATGAAGAACATTGGATTAGGAACGTCAGCGTATCCACTCGACA 5 0.125 No Hit CCTCAATCTTCATTTCTAAAACCCCAATATTGAAACTTTAAACTACCTCC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0125 0.0 0.0 0.0 0.0 74-75 0.0625 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.3125 0.0 0.0 0.0 0.0 86-87 0.4375 0.0 0.0 0.0 0.0 88-89 0.475 0.0 0.0 0.0 0.0 90-91 0.5625 0.0 0.0 0.0 0.0 92-93 0.7375 0.0 0.0 0.0 0.0 94-95 0.925 0.0 0.0 0.0 0.0 96-97 1.125 0.0 0.0 0.0 0.0 98-99 1.3625 0.0 0.0 0.0 0.0 100-101 1.7875 0.0 0.0 0.0 0.0 102-103 2.0 0.0 0.0 0.0 0.0 104-105 2.2 0.0 0.0 0.0 0.0 106-107 2.45 0.0 0.0 0.0 0.0 108-109 2.9124999999999996 0.0 0.0 0.0 0.0 110-111 3.4625000000000004 0.0 0.0 0.0 0.0 112-113 3.9625 0.0 0.0 0.0 0.0 114-115 4.387499999999999 0.0 0.0 0.0 0.0 116-117 4.8 0.0 0.0 0.0 0.0 118-119 5.3625 0.0 0.0 0.0 0.0 120-121 5.9 0.0 0.0 0.0 0.0 122-123 6.324999999999999 0.0 0.0 0.0 0.0 124-125 6.8375 0.0 0.0 0.0 0.0 126-127 7.175 0.0 0.0 0.0 0.0 128-129 7.5125 0.0 0.0 0.0 0.0 130-131 8.1375 0.0 0.0 0.0 0.0 132-133 8.662500000000001 0.0 0.0 0.0 0.0 134-135 9.525 0.0 0.0 0.0 0.0 136-137 10.4375 0.0 0.0 0.0 0.0 138-139 11.375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTTTT 10 0.006830828 145.0 3 TCCAGAT 10 0.006830828 145.0 7 GGGATGT 10 0.006830828 145.0 3 >>END_MODULE SRR26075399 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075399_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.326 37.0 37.0 37.0 37.0 37.0 2 36.345 37.0 37.0 37.0 37.0 37.0 3 36.43 37.0 37.0 37.0 37.0 37.0 4 36.3965 37.0 37.0 37.0 37.0 37.0 5 36.496 37.0 37.0 37.0 37.0 37.0 6 36.418 37.0 37.0 37.0 37.0 37.0 7 36.339 37.0 37.0 37.0 37.0 37.0 8 36.4085 37.0 37.0 37.0 37.0 37.0 9 36.455 37.0 37.0 37.0 37.0 37.0 10-14 36.34570000000001 37.0 37.0 37.0 37.0 37.0 15-19 36.323800000000006 37.0 37.0 37.0 37.0 37.0 20-24 36.277499999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.2251 37.0 37.0 37.0 37.0 37.0 30-34 36.0981 37.0 37.0 37.0 37.0 37.0 35-39 36.07809999999999 37.0 37.0 37.0 37.0 37.0 40-44 36.09160000000001 37.0 37.0 37.0 37.0 37.0 45-49 35.987700000000004 37.0 37.0 37.0 37.0 37.0 50-54 35.8887 37.0 37.0 37.0 37.0 37.0 55-59 35.97019999999999 37.0 37.0 37.0 37.0 37.0 60-64 35.9499 37.0 37.0 37.0 37.0 37.0 65-69 35.9294 37.0 37.0 37.0 37.0 37.0 70-74 35.819900000000004 37.0 37.0 37.0 37.0 37.0 75-79 35.687200000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.7924 37.0 37.0 37.0 37.0 37.0 85-89 35.752300000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.630199999999995 37.0 37.0 37.0 37.0 37.0 95-99 35.7051 37.0 37.0 37.0 37.0 37.0 100-104 35.676 37.0 37.0 37.0 37.0 37.0 105-109 35.6471 37.0 37.0 37.0 37.0 37.0 110-114 35.521 37.0 37.0 37.0 37.0 37.0 115-119 35.50750000000001 37.0 37.0 37.0 37.0 37.0 120-124 35.3811 37.0 37.0 37.0 37.0 37.0 125-129 35.3928 37.0 37.0 37.0 37.0 37.0 130-134 35.3434 37.0 37.0 37.0 34.6 37.0 135-139 35.235400000000006 37.0 37.0 37.0 29.8 37.0 140-144 35.20105 37.0 37.0 37.0 34.6 37.0 145-149 35.243050000000004 37.0 37.0 37.0 32.2 37.0 150-151 34.898875000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 3.0 14 6.0 15 3.0 16 2.0 17 4.0 18 2.0 19 5.0 20 1.0 21 6.0 22 9.0 23 7.0 24 8.0 25 9.0 26 13.0 27 13.0 28 8.0 29 22.0 30 11.0 31 34.0 32 45.0 33 81.0 34 195.0 35 629.0 36 2672.0 37 211.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.7 21.275 11.625 28.4 2 27.925 27.675 27.474999999999998 16.925 3 21.4 29.725 29.275000000000002 19.6 4 24.2 33.15 24.175 18.475 5 26.275 35.925000000000004 20.474999999999998 17.325 6 22.25 39.1 20.349999999999998 18.3 7 23.075000000000003 23.525 34.599999999999994 18.8 8 23.125 27.650000000000002 25.15 24.075 9 24.55 24.525 29.2 21.725 10-14 24.025 29.175 25.16 21.64 15-19 24.38 27.889999999999997 26.115 21.615000000000002 20-24 24.09 28.59 26.669999999999998 20.65 25-29 24.335 28.87 26.32 20.474999999999998 30-34 24.529999999999998 28.299999999999997 26.674999999999997 20.495 35-39 24.145 28.205000000000002 26.729999999999997 20.919999999999998 40-44 24.415 27.165 27.24 21.18 45-49 24.39 27.415 27.310000000000002 20.885 50-54 24.435000000000002 28.175 27.26 20.13 55-59 24.060000000000002 28.77 26.35 20.82 60-64 24.060000000000002 28.38 26.669999999999998 20.89 65-69 23.94 28.53 26.900000000000002 20.630000000000003 70-74 24.46 27.744999999999997 26.5 21.295 75-79 24.060000000000002 28.349999999999998 26.924999999999997 20.665 80-84 24.205 27.71 27.255000000000003 20.830000000000002 85-89 24.34 28.505000000000003 26.185000000000002 20.97 90-94 24.615000000000002 29.060000000000002 26.640000000000004 19.685 95-99 24.55 29.595 26.0 19.855 100-104 24.615000000000002 27.83 27.139999999999997 20.415 105-109 24.815 28.035 27.134999999999998 20.015 110-114 24.37 28.610000000000003 26.474999999999998 20.544999999999998 115-119 25.405 28.425 26.235000000000003 19.935 120-124 25.39 28.92 25.64 20.05 125-129 25.44 28.044999999999998 26.58 19.935 130-134 25.935000000000002 28.73 25.480000000000004 19.855 135-139 26.25 28.000000000000004 25.855 19.895 140-144 26.45632281614081 28.096404820241013 25.891294564728234 19.555977798889945 145-149 25.95889383407511 29.164374656198426 25.393809071360707 19.482922438365755 150-151 28.041005125640705 27.365920740092513 25.965745718214777 18.627328416052006 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.5 5 1.5 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 1.0 16 0.5 17 1.0 18 0.5 19 0.0 20 0.0 21 1.0 22 2.0 23 1.5 24 1.5 25 1.5 26 3.0 27 5.5 28 8.5 29 7.5 30 6.5 31 15.5 32 22.5 33 21.5 34 31.5 35 45.5 36 59.0 37 87.5 38 132.0 39 178.5 40 190.0 41 203.5 42 233.5 43 256.0 44 267.5 45 274.0 46 274.5 47 267.0 48 242.5 49 204.5 50 189.0 51 143.5 52 117.5 53 106.0 54 79.0 55 70.5 56 51.5 57 29.5 58 26.5 59 28.5 60 19.0 61 12.0 62 6.0 63 4.5 64 4.5 65 6.0 66 5.5 67 4.0 68 4.0 69 3.5 70 1.5 71 2.5 72 3.5 73 1.5 74 3.0 75 3.5 76 1.0 77 0.5 78 0.5 79 0.5 80 2.0 81 1.5 82 1.0 83 1.0 84 0.5 85 0.5 86 1.0 87 1.0 88 0.0 89 0.0 90 1.5 91 1.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.015 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.125 #Duplication Level Percentage of deduplicated Percentage of total 1 63.903420523138834 39.7 2 21.81086519114688 27.1 3 8.531187122736418 15.9 4 3.2595573440643864 8.1 5 1.4486921529175052 4.5 6 0.5231388329979879 1.95 7 0.2012072434607646 0.8750000000000001 8 0.16096579476861167 0.8 9 0.04024144869215292 0.22499999999999998 >10 0.12072434607645875 0.8500000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 12 0.3 No Hit GATGAAGATGATGAAGACATCGATGCAGAGGTAGAAGAGGACGACGGAGA 11 0.27499999999999997 No Hit GAAAGAACACAACAACAAACAACAACTACTTCTTAAAACTATAAAACTCT 11 0.27499999999999997 No Hit CGATGATGTACGGGAAGCCACCCAAGAAGAAGGGAGGTATGGGAGGCGCA 9 0.22499999999999998 No Hit TTGCCCACCCAAATTGTTGTTTGACAGCTTGAGAAACCATATTGTTGTTA 8 0.2 No Hit AAGCTCGGATCTATAGCTATTGAAGCTGCTCTTAAAAGGGCCAATGTTGA 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 8 0.2 No Hit ATTCACTGTAGCTTCTAAATTCAAAAGGAGCTATTAGCTTTTGATAGAAA 8 0.2 No Hit AGAAAGAAAATCCGGAAGCTTGTGATCTGTGCATGGAGATGCAACCTTTA 7 0.17500000000000002 No Hit GTCCAAAAGAGCAGAATCTTCTTCATTGCATGGTGTCCCGACACATCAAG 7 0.17500000000000002 No Hit CGAAGCGAAACAGAACAGAACAAAACCAGAGTAAAGACAGAGAGGGTCCT 7 0.17500000000000002 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 7 0.17500000000000002 No Hit ATTGGATAACCTCCCTAGTGGAACCATAATAAGAAAAATTCAACCAAGCA 7 0.17500000000000002 No Hit GCCCTCTTTCTCTATCATCTCTCTCGGCCTCTCTTTGCTTCAAATCGAGA 6 0.15 No Hit AAGCGTCAGAAGAGAACCTTGGTCCACTTGAAGGACAAGTACAATGAACT 6 0.15 No Hit TTCTATTGCTGGGATACAGCTGGTCAAGAGAAGTTTGGTGGTCTTCGAGA 6 0.15 No Hit AAATGAGCTCACCTAATCACAGTGGAAAGAAAGAAAAACCCAATCATCAT 6 0.15 No Hit CCCCTGTAAAGCCTTGCTGCAGTGTCATTCAAGGCCTTCTTGATCTCGAG 6 0.15 No Hit GCATGTCAAGTCAGGGTTATGGCCAGCTTGGGCAAGACAGTTTTTAGCAT 6 0.15 No Hit GTTCCTGATCAATGGTCTTCATGAACTGAATCATTGCATTCTTTTCATTC 6 0.15 No Hit CCAGATCAAGAGCTTCTTGCAATCGTGAAGAGTTTTGAACATTGGAGACA 6 0.15 No Hit CATGGACCAGCCCGAGGCTTCATTAGAGATGCTGAAGAGGTGGACTCGGG 6 0.15 No Hit CGTAATGGTTCGGCTGCAGGGAGACTTCGCGAAGCCCCCAGAGCAGAGGT 6 0.15 No Hit CTTTATTTTATTTTTCTGGTTTCAAAATTCAGTCAACTGAGGAGGTTTGA 6 0.15 No Hit GAAGATTGGCCTACTGTGACAGAGATGGTGCAAGAAAATCACAGGCTTGT 6 0.15 No Hit GTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAG 6 0.15 No Hit GCGTCCTCAGCGGCGGACCAGGCCCAAGTCCCCTGGAAAGGGGCGCCGGA 5 0.125 No Hit AGAGAGAGGATACGATCCAGAAAATTTAATGATATGGGGAGCGCTAGCAG 5 0.125 No Hit TTAAAAAGTACGTGAAACAATTGAAAAGGAAGCACTTGTGACCAGACTTG 5 0.125 No Hit CCAAAGGTTCAGACCATTTAAGGGATGTCTTTGGCCATATGGGTCTTAGT 5 0.125 No Hit CATCAAACAGCTATCTCGGTTCCTGTCCAATCTAATGGTGGTTGTCATGC 5 0.125 No Hit ATCACAAGAAAGTCTCGTCTTCTTGATAACGAGATTCGCATTCTCAAGGA 5 0.125 No Hit GAGCAGTACAAGAGGGAACACCCTAAAAACAAATCCGTTGCAGCTGTTGG 5 0.125 No Hit TGAACGGGCAGAAAAAAGTACGAAGGACCAACAAGTCTGTTGGCAGAAAT 5 0.125 No Hit GGTATTTTGAGCAGTTTGGAGAGATCAAAGAAGCAGTTGTGATCATTGAT 5 0.125 No Hit TGCAAATCTTTGTTAAGACTTTGACTGGAAAGACCATCACCCTGGAGGTG 5 0.125 No Hit CTGAAATTGAAAAGGCAAAACTGGAATCGCAGACGCTTGAGTCAGTTTTG 5 0.125 No Hit CTTCTCTGGTCTCTCTCTCAGCATCCAAATCCTAACCAGACTTTCTTAAG 5 0.125 No Hit AAGAAAATGCTATTCTGATGAATGCCTGCCCGGAAAACCCAAAGTTGTCT 5 0.125 No Hit AACTGTCCTCCTATACCTAACGCAATTTAACCGGAGAGGAATCGGATTTT 5 0.125 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAA 5 0.125 No Hit CGGGATCTTAAACCTGAGAATTTCTTGTTCACATCAAAGGATGAAAACTC 5 0.125 No Hit CCTGCTCTTACCATCTCTAAGGTACTGCTCTCCATTTGCTCATTGCTGAC 5 0.125 No Hit GTTTTCAGACATATGTGCGACCAACATGCAATCAGCACCTCAGTGATTTC 5 0.125 No Hit GAGGTTTAGCATTTCTCCATGAAGGATCTGCTATCAGGATTGTTCACAGG 5 0.125 No Hit CTTACACATGCAAGTCGAACGGTAACAGGGAGCTTGCTCCGCTGACGAGT 5 0.125 No Hit CCGCAGCAGCTCGTTACAATGCTCCAGTAGTCTTGAAGGACTCCCCCACC 5 0.125 No Hit GCATCGAGCTAAAGAGTGCACAGCTCCACCCATGCCACCTGGGGACTTGA 5 0.125 No Hit GGCTTGGCCAAGTTTCCATTTCAAATGCCTCTAGGCGACGACTTGCTGGA 5 0.125 No Hit CAGAACAGCAAGCTCGAAGAGCTTGGGAGGTGGTAGCAGTAGGGCAAGAG 5 0.125 No Hit AGAAACCCAAAGTGCAACTAAAACCCAAAAAAAATGTCTAAGCTTACAAC 5 0.125 No Hit GTACATTGTTAGCAGAAAATACCCTGGGTGCAAACCATGACCACTTCTTT 5 0.125 No Hit ATTCAGTGTTGTAGAAGAGAGACCATGTGTGCTTGTTTTATTTTGCTAAA 5 0.125 No Hit TTTCCTCACTCAAAACCACCACGAAGAAGCCTTTAGCCTCTGGTTTTTCT 5 0.125 No Hit AAATTCTCTTCCTCTCTCTCTATTCTAGACTTTCCGTCTCCGTCTCTCTG 5 0.125 No Hit TGAAGAGGCACCTGCACCAGTGGAGGAGAGAAGGCTTGCTACCTGGGGAA 5 0.125 No Hit GTGCAAGATCAACCTCAACTCTTGCGATACCACGATGGGGCTCTTCTTTA 5 0.125 No Hit ATCTTATCTCTGCAAAAATGGGGATTTCTCGTGATTCTATGCACAAGAGG 5 0.125 No Hit GATCACATCTACTTCTACAGCTGCCTTTCTTGAAAGCAACTCGAGCCCCA 5 0.125 No Hit ACACCTTAAAGCTTCTGAGTTGTCACTGAGGAATGCAGGAGAGAAGGGTT 5 0.125 No Hit CCCCAATCCTCATCCGCCAAGCCAAATTCCTGTAACAAAAGTTATCGACA 5 0.125 No Hit AAGAAACATAGGGACAGAATCAACTTTTGCAGAGCAATGAATAGCGGTAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0125 0.0 0.0 0.0 0.0 74-75 0.0625 0.0 0.0 0.0 0.0 76-77 0.0875 0.0 0.0 0.0 0.0 78-79 0.15000000000000002 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.25 0.0 0.0 0.0125 0.0 84-85 0.3375 0.0 0.0 0.025 0.0 86-87 0.475 0.0 0.0 0.025 0.0 88-89 0.5375000000000001 0.0 0.0 0.025 0.0 90-91 0.6375 0.0 0.0 0.025 0.0 92-93 0.8125 0.0 0.0 0.025 0.0 94-95 1.0 0.0 0.0 0.025 0.0 96-97 1.2000000000000002 0.0 0.0 0.025 0.0 98-99 1.4375 0.0 0.0 0.025 0.0 100-101 1.8624999999999998 0.0 0.0 0.025 0.0 102-103 2.075 0.0 0.0 0.025 0.0 104-105 2.2750000000000004 0.0 0.0 0.025 0.0 106-107 2.5125 0.0 0.0 0.025 0.0 108-109 2.9625000000000004 0.0 0.0 0.025 0.0 110-111 3.5374999999999996 0.0 0.0 0.025 0.0 112-113 4.0375 0.0 0.0 0.025 0.0 114-115 4.4625 0.0 0.0 0.025 0.0 116-117 4.8875 0.0 0.0 0.025 0.0 118-119 5.4625 0.0 0.0 0.025 0.0 120-121 6.0 0.0 0.0 0.025 0.0 122-123 6.425000000000001 0.0 0.0 0.025 0.0 124-125 6.9125 0.0 0.0 0.025 0.0 126-127 7.25 0.0 0.0 0.025 0.0 128-129 7.6625 0.0 0.0 0.025 0.0 130-131 8.275 0.0 0.0 0.025 0.0 132-133 8.8125 0.0 0.0 0.025 0.0 134-135 9.6875 0.0 0.0 0.025 0.0 136-137 10.625 0.0 0.0 0.025 0.0 138-139 11.575 0.0 0.0 0.025 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra Read 386713 spots for SRR26075399.sra Written 386713 spots for SRR26075399.sra Read 386699 spots for SRR26075399.sra Written 386699 spots for SRR26075399.sra SRR ids: ['SRR26075399.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_qi7py7kw SRR26075399.sra spots: 7733994 blocks: [[1, 386699], [386700, 773398], [773399, 1160097], [1160098, 1546796], [1546797, 1933495], [1933496, 2320194], [2320195, 2706893], [2706894, 3093592], [3093593, 3480291], [3480292, 3866990], [3866991, 4253689], [4253690, 4640388], [4640389, 5027087], [5027088, 5413786], [5413787, 5800485], [5800486, 6187184], [6187185, 6573883], [6573884, 6960582], [6960583, 7347281], [7347282, 7733994]] SRR26075399 file size 2849808 SRR26075399 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075399 SRR26075399_1.fastq SRR26075399_2.fastq Input file: SRR26075399_1.fastq Paired file: SRR26075399_2.fastq trimmed: SRR26075399-trimmed-pair1.fastq, SRR26075399-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:01:45 2025 >> started Wed Feb 12 01:01:53 2025 >> done (8.933s) 7733994 read pairs processed; of these: 15 ( 0.00%) short read pairs filtered out after trimming by size control 10673 ( 0.14%) empty read pairs filtered out after trimming by size control 7723306 (99.86%) read pairs available; of these: 1241915 (16.08%) trimmed read pairs available after processing 6481391 (83.92%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 0 0.00% 20 2 0.00% 21 6 0.00% 22 1 0.00% 23 3 0.00% 24 4 0.00% 25 6 0.00% 26 3 0.00% 27 7 0.00% 28 6 0.00% 29 2 0.00% 30 9 0.00% 31 9 0.00% 32 3 0.00% 33 6 0.00% 34 9 0.00% 35 6 0.00% 36 11 0.00% 37 6 0.00% 38 10 0.00% 39 13 0.00% 40 9 0.00% 41 34 0.00% 42 13 0.00% 43 16 0.00% 44 11 0.00% 45 17 0.00% 46 18 0.00% 47 14 0.00% 48 43 0.00% 49 34 0.00% 50 25 0.00% 51 33 0.00% 52 37 0.00% 53 46 0.00% 54 68 0.00% 55 45 0.00% 56 68 0.00% 57 86 0.00% 58 101 0.00% 59 126 0.00% 60 130 0.00% 61 143 0.00% 62 142 0.00% 63 205 0.00% 64 233 0.00% 65 245 0.00% 66 275 0.00% 67 293 0.00% 68 333 0.00% 69 419 0.01% 70 461 0.01% 71 576 0.01% 72 605 0.01% 73 745 0.01% 74 818 0.01% 75 999 0.01% 76 1136 0.01% 77 1267 0.02% 78 1357 0.02% 79 1475 0.02% 80 1724 0.02% 81 1949 0.03% 82 2346 0.03% 83 2488 0.03% 84 2843 0.04% 85 3236 0.04% 86 3473 0.04% 87 3731 0.05% 88 3885 0.05% 89 4172 0.05% 90 4626 0.06% 91 5253 0.07% 92 5352 0.07% 93 5921 0.08% 94 6369 0.08% 95 6944 0.09% 96 7320 0.09% 97 8146 0.11% 98 8338 0.11% 99 8614 0.11% 100 9154 0.12% 101 9348 0.12% 102 10003 0.13% 103 10631 0.14% 104 11390 0.15% 105 12158 0.16% 106 12623 0.16% 107 13473 0.17% 108 13848 0.18% 109 14106 0.18% 110 14584 0.19% 111 14506 0.19% 112 15484 0.20% 113 15751 0.20% 114 17021 0.22% 115 17142 0.22% 116 17799 0.23% 117 18743 0.24% 118 19085 0.25% 119 19275 0.25% 120 19831 0.26% 121 20261 0.26% 122 20868 0.27% 123 21223 0.27% 124 21899 0.28% 125 22111 0.29% 126 22437 0.29% 127 23529 0.30% 128 24201 0.31% 129 24983 0.32% 130 25772 0.33% 131 25597 0.33% 132 25711 0.33% 133 26694 0.35% 134 26704 0.35% 135 27977 0.36% 136 27882 0.36% 137 28255 0.37% 138 29245 0.38% 139 29602 0.38% 140 29766 0.39% 141 30461 0.39% 142 30846 0.40% 143 31184 0.40% 144 30792 0.40% 145 31707 0.41% 146 32187 0.42% 147 33215 0.43% 148 33569 0.43% 149 33645 0.44% 150 34081 0.44% 151 6481391 83.92% 7723306 reads passed initial QC criterion=sequence-density sequence-density=0.17 sequence-density-rank=1 fanout-score=3.56 fanout-score-rank=24 prefix-density=0.20 prefix-fanout=2.9 sequence=CAGGTGCAGTTTGATCCACA criterion=fanout-score sequence-density=0.06 sequence-density-rank=31 fanout-score=345.66 fanout-score-rank=1 prefix-density=0.76 prefix-fanout=28.0 sequence=TCATCATCACCACCATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTC criterion=sequence-density sequence-density=0.41 sequence-density-rank=1 fanout-score=2.16 fanout-score-rank=35 prefix-density=0.41 prefix-fanout=2.2 sequence=TGGTTTTACTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=93.31 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=9.3 sequence=AAAGCAGTTGCATTTATCTAAAGTATTCTCACTTTACTTAACACTTGAGCTACATAGAATGTCTACAGTCAATTTGGCAGCACTGCTGTTGCTTGGGCTGCTGCTGGTTATGCCACAGCAGTCCATGCAAGCGAGTTTGATAGACCCCATTGCTGAAATCGAGAGAAGCAACTGCAAAATCGCACACCTTCGCTTAGGGCTTGTTTTTACGTCTGATAACAACGAAAGGGCTCTGCAAGACTCTGGACTCTATAGCCCTGACAGTGAAGACTCTTCTGTTGACATTGCGGGCAGAAGGTTCCATAGCGGGACACTAAACGGTTCTTCTATTGTATATGTCAAGACAGGGAGTCATTCAGTAAATATGGCAACAACTTTGCAAATCCTT SRR26075399 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:02:43 Started mapping on | Feb 12 01:02:43 Finished on | Feb 12 01:04:43 Mapping speed, Million of reads per hour | 231.70 Number of input reads | 7723306 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 6721456 Uniquely mapped reads % | 87.03% Average mapped length | 292.75 Number of splices: Total | 6397428 Number of splices: Annotated (sjdb) | 6229615 Number of splices: GT/AG | 6276305 Number of splices: GC/AG | 93344 Number of splices: AT/AC | 6476 Number of splices: Non-canonical | 21303 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 3.03 Insertion rate per base | 0.02% Insertion average length | 2.58 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 193581 % of reads mapped to multiple loci | 2.51% Number of reads mapped to too many loci | 70539 % of reads mapped to too many loci | 0.91% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 9.26% % of reads unmapped: other | 0.30% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 808269 808269 808269 N_multimapping 193581 193581 193581 N_noFeature 186805 6637827 226627 N_ambiguous 79417 398 35425 UnstrandedReadsAssigned:6455234 PositiveStrandReadsAssigned:83231 NegativeStrandReadsAssigned:6459404 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075399 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075399-trimmed-pair1.fastq SRR26075399-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 7,723,306 reads, 6,562,559 reads pseudoaligned [quant] estimated average fragment length: 217.115 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,013 rounds 52401 SRR26075399.ke.tsv 34699 SRR26075399.se.tsv 87100 total ==> SRR26075399.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1801.88 696 49.5214 Potri.005G024800.1.v4.1 1035 818.885 307 48.0647 Potri.004G059700.1.v4.1 961 744.896 0 0 Potri.007G009000.2.v4.1 1416 1199.88 0 0 Potri.003G141000.2.v4.1 2943 2726.88 287 13.4935 Potri.016G087400.1.v4.1 270 89.4485 526 753.916 Potri.015G069301.1.v4.1 564 350.329 0 0 Potri.010G195200.1.v4.1 1773 1556.88 63 5.18793 Potri.012G127500.1.v4.1 977 760.896 4775 804.56 ==> SRR26075399.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 62 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 150 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 256 SRR26075399 completed mapping pipeline successfully