Starting /dee2/code/volunteer_pipeline.sh SRR26075400 current disk space = 3051262881792 free memory = 1187657644 SRR26075400 SRAfilesize af75e1bf4687e27ebec26af0545462d6 SRR26075400.sra SRR26075400.sra file validated SRR26075400 is paired end SRR26075400 is conventional basespace SRR26075400 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075400_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.44175 37.0 37.0 37.0 37.0 37.0 2 36.668 37.0 37.0 37.0 37.0 37.0 3 36.656 37.0 37.0 37.0 37.0 37.0 4 36.7125 37.0 37.0 37.0 37.0 37.0 5 36.724 37.0 37.0 37.0 37.0 37.0 6 36.714 37.0 37.0 37.0 37.0 37.0 7 36.5605 37.0 37.0 37.0 37.0 37.0 8 36.646 37.0 37.0 37.0 37.0 37.0 9 36.6955 37.0 37.0 37.0 37.0 37.0 10-14 36.591300000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.6364 37.0 37.0 37.0 37.0 37.0 20-24 36.537400000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.49759999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.4705 37.0 37.0 37.0 37.0 37.0 35-39 36.434000000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.3989 37.0 37.0 37.0 37.0 37.0 45-49 36.3294 37.0 37.0 37.0 37.0 37.0 50-54 36.2649 37.0 37.0 37.0 37.0 37.0 55-59 36.2054 37.0 37.0 37.0 37.0 37.0 60-64 36.1623 37.0 37.0 37.0 37.0 37.0 65-69 36.206300000000006 37.0 37.0 37.0 37.0 37.0 70-74 36.181200000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.0263 37.0 37.0 37.0 37.0 37.0 80-84 36.050399999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.9154 37.0 37.0 37.0 37.0 37.0 90-94 35.897299999999994 37.0 37.0 37.0 37.0 37.0 95-99 35.8642 37.0 37.0 37.0 37.0 37.0 100-104 35.7985 37.0 37.0 37.0 37.0 37.0 105-109 35.7078 37.0 37.0 37.0 37.0 37.0 110-114 35.524 37.0 37.0 37.0 37.0 37.0 115-119 35.507600000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.6259 37.0 37.0 37.0 37.0 37.0 125-129 35.446299999999994 37.0 37.0 37.0 37.0 37.0 130-134 35.281699999999994 37.0 37.0 37.0 29.8 37.0 135-139 35.096799999999995 37.0 37.0 37.0 29.8 37.0 140-144 34.9697 37.0 37.0 37.0 25.0 37.0 145-149 34.9411 37.0 37.0 37.0 25.0 37.0 150-151 34.804249999999996 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 4.0 24 7.0 25 3.0 26 7.0 27 8.0 28 20.0 29 23.0 30 31.0 31 45.0 32 67.0 33 115.0 34 213.0 35 479.0 36 2770.0 37 207.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.13445589344057 14.350339281226438 8.56999246041719 41.945212364915804 2 19.400000000000002 14.674999999999999 35.55 30.375000000000004 3 17.549999999999997 15.075 27.500000000000004 39.875 4 22.925 24.125 23.3 29.65 5 24.7 27.1 24.175 24.025 6 22.45 33.324999999999996 21.55 22.675 7 16.900000000000002 29.75 38.1 15.25 8 18.725 28.175 31.15 21.95 9 20.825 22.1 34.2 22.875 10-14 19.825 29.425 28.189999999999998 22.56 15-19 19.134999999999998 28.544999999999998 27.88 24.44 20-24 19.919999999999998 27.77 28.955 23.355 25-29 20.355 27.474999999999998 28.65 23.52 30-34 19.935 27.935 27.384999999999998 24.745 35-39 20.560000000000002 27.994999999999997 27.07 24.375 40-44 20.255000000000003 27.439999999999998 27.07 25.235000000000003 45-49 20.59 27.1 27.315 24.995 50-54 20.915 27.57 27.500000000000004 24.015 55-59 20.19 26.87 28.235 24.705 60-64 20.415 28.055000000000003 27.82 23.71 65-69 20.565 27.37 28.115000000000002 23.95 70-74 20.805 26.0 28.244999999999997 24.95 75-79 20.275000000000002 27.950000000000003 27.52 24.255 80-84 21.3 26.700000000000003 27.634999999999998 24.365000000000002 85-89 21.015 27.68 27.875 23.43 90-94 21.240000000000002 27.98 26.565 24.215 95-99 20.69 28.21 26.825 24.275 100-104 21.34 27.675 26.68 24.305 105-109 21.175 27.405 27.83 23.59 110-114 21.37 27.76 27.315 23.555 115-119 21.67 26.979999999999997 27.265 24.085 120-124 21.305 28.365000000000002 25.71 24.62 125-129 20.995 27.24 26.810000000000002 24.955 130-134 21.21 28.199999999999996 27.13 23.46 135-139 21.715 27.689999999999998 27.034999999999997 23.56 140-144 22.185 28.15 25.635 24.03 145-149 22.065 28.139999999999997 25.585 24.21 150-151 23.0625 27.675 25.9875 23.275000000000002 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 1.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 2.5 24 2.0 25 0.5 26 6.5 27 12.0 28 9.0 29 6.5 30 6.5 31 14.0 32 19.0 33 21.0 34 41.0 35 61.0 36 86.5 37 93.0 38 102.5 39 140.0 40 173.5 41 192.5 42 223.5 43 254.5 44 268.5 45 286.0 46 266.0 47 253.5 48 253.0 49 238.0 50 204.0 51 152.5 52 116.5 53 96.0 54 81.5 55 56.0 56 47.0 57 48.0 58 31.5 59 25.5 60 25.0 61 16.5 62 10.5 63 10.0 64 8.0 65 2.5 66 5.5 67 8.5 68 7.0 69 5.5 70 2.0 71 1.5 72 1.5 73 1.0 74 1.0 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.525 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.324999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 63.07501036054703 38.05 2 21.591380024865316 26.05 3 8.164111065064235 14.774999999999999 4 3.937007874015748 9.5 5 1.740571902196436 5.25 6 0.7459593866556155 2.7 7 0.24865312888520513 1.05 8 0.29009531703273933 1.4000000000000001 9 0.08288437629506838 0.44999999999999996 >10 0.12432656444260257 0.775 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTGCCACTGACGCTCGTGAATGTGCCGTTTTCCCATTGAAAATACATTT 11 0.27499999999999997 No Hit GCTCCAATGTCTATGCCCAGACCTTTCAGTATCTCAGGTACTTCAGCTGC 10 0.25 No Hit TCCCCATCTCCATCTTAAAGTAACCGTTGTCGCCCCAGTCTTCTCCCCAA 10 0.25 No Hit CTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGAC 9 0.22499999999999998 No Hit CACCCAAACCCAAGAGGCCATTAAAACAAAGGCCTCAAAAGCAAAACATA 9 0.22499999999999998 No Hit AGGAGACTTCAAGCTTGGGGGCAAGAACGCTGGCTTTAGAAGGTGGAACC 8 0.2 No Hit ATTGTATGTCCCTGCATCATCTGGCAATTCCCATTCAGGGTGGCCTGCAT 8 0.2 No Hit GCAGAAAAGAAACACTCTAATTCCTCAAACCATGTCTCGACGCAGAGTTA 8 0.2 No Hit GTCCAGGAGCACGACCACGGCCAGGAGCTTGACCACGGCCATTCCTCGTA 8 0.2 No Hit CTTGGAACACCACGAAGAGCTTTTCATTTGACAAAGATGTCAAAGCTACA 8 0.2 No Hit ATCAGCTTAAACCAATTGAGTTCTCACATTAGACACAAGACCTCTCCGAT 8 0.2 No Hit GTTTGAAATAGTGAATTATTTTGGGCTTCTGGCTCTCATAGCCCACATTT 8 0.2 No Hit CTTCAAAACTTGCAACAATTTTCCAACCAGAACCCTCACCCGTCATAATG 7 0.17500000000000002 No Hit TGAGTTTTTCATCATTGATGCTATCATCTAGATTCTTGATATACAAATTC 7 0.17500000000000002 No Hit CACTGGTAGAAAGGACCTCGACCTCCTCCACACCTAATGCATGGAAGCAG 7 0.17500000000000002 No Hit CCTCACTGAAGTTCAGTGACCTCCTGGACTTTCTCAGCTTTGGGGAACCA 7 0.17500000000000002 No Hit AGCGTAGTGGCTAATACTATAATATATAGTGCTAACTTTATACTATTCTT 7 0.17500000000000002 No Hit CTCCGATTGTGTACTGCCAAATCCTGATAGAGCCCGCAGTCTCCCAGTCA 7 0.17500000000000002 No Hit AGACTGTTGATCTCCATTTCTAATAAATAGCCTAATAAGCATAACCACCT 6 0.15 No Hit GTCTTTTTCCATGGTAAAGGCATGTTGGGGTTAGAAAAGATTGGATTTTG 6 0.15 No Hit AACCACCTTCCGTATCATGAATGGGGAATGTATACTGTAGTATTCGTCCC 6 0.15 No Hit GCTGTTTTGGTTGTCTATTTGCAACAACCATCCTCCTAGACCCACTTCTT 6 0.15 No Hit CTCCTGGTGGTAATGTTAAGAACCTTGGTAGGGATTCTCACTGGACCCTT 6 0.15 No Hit CCCATCAATTCATGAGAAGGAACCTCAGTATCCTTCTGCAGAGGAATCAA 6 0.15 No Hit GTCCACATAATCACCTAAGAATAAATAATTGGCACTGGGAGGAAAGCCTC 6 0.15 No Hit CCCTCATCCACCTGCCATCTTTCCACACCGCGTCCGTCGTCCACCGGCGC 6 0.15 No Hit GGCCGGTAGAATATTACAAGAAGCCATGCAAAGCAACCAACCATTGAAGA 6 0.15 No Hit AGCCAGGGCATTTTATTTTGTTATATATAACACACATGATTGCGAGAATA 6 0.15 No Hit CGTATTTTGCCGCTTCCAGGTGGGCTCTCAATGCATGAATTTCCCGATCA 6 0.15 No Hit GTGACGGTGAGTTTGACGAACATTTTGAGATTAAAAGGTTATGGTCAATG 6 0.15 No Hit CCCCGCTACCCAATGAAAGGAGTATCATTTTGAGCAATGCAACCTGCATA 6 0.15 No Hit TTCCATATATTATTTCCATTCAACCCCCAAATCATAGTTTGGAGCTGCAC 6 0.15 No Hit GCCACTCTCATTTTTTCCTTCTGGGAATTCATAACTGCAAATTCCCTAAT 6 0.15 No Hit GTGATCTTCCTTTTCCGATTAAAAGCAAATTGATAAGAGATGCACAGGGT 6 0.15 No Hit GCCAAGTATTGCACCCAGAAGTACAACCCCCTATGGTTCCACCATTAACA 6 0.15 No Hit CGGCCTCTTCCTCATATCCAAACAATTGTTGACAGCAAAATCATAAGTTT 6 0.15 No Hit TCCAGGGTGATGGTTTTTCCAGTCAGGGTCTTGACAAAGATTTGCATTCC 5 0.125 No Hit GTTCTGGCTTGGCTGTACTCTTGAGACCACACAACCTTTCGGGAGATTTT 5 0.125 No Hit GGAAGCATTGCGAGAGGAGAAACTTGGGAAAGGAGAGAAAGGTCGGCCAT 5 0.125 No Hit CCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTG 5 0.125 No Hit TCCTTTTCGAAAGAGGGATCCCAATGGTTTAATTGAAACCATTAGAAGCA 5 0.125 No Hit CTCATTAAGATCAACTGCAGAGGACTCAACTGGTAAGTTCAAATCAACTG 5 0.125 No Hit CATGAAAGGAAACATATGTTTATTAACAAACCGGGAAGCTTCAGGACCAC 5 0.125 No Hit TGCACATTTGTGGTCCTTGTGGTGACCGCTGTCAGTTGACTCGCTTCTTT 5 0.125 No Hit CGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTT 5 0.125 No Hit GCCTCTCTCGATCACGCTCATAGTCCCGACCACGGTCCCGACCTCGATCA 5 0.125 No Hit GTTGGATCCATGAAGTTGGACAGCACTGGGGTCACAGACCGGTCACTTGG 5 0.125 No Hit CCCAGAACCTCATAATACTTGGTGTTGTCACTCCTCCTTGGAGCACGCCC 5 0.125 No Hit CCGAAAGAGAACACATCAACCTTTTCAGAAACCTTACTGCTGCTACCATT 5 0.125 No Hit ATCCCACACCTGTTTCTTGAGCTGCTGTTGCTTAGCCTTTTTCTCCTGGA 5 0.125 No Hit GCTCTATATAACGACTCCCAAGAGTCATCCTATCCTTGGCCATCGCTGCT 5 0.125 No Hit CCGGAACCCGACTCAAACCAACCCATGTTTCCTTGATTTCTCCAGCTCCA 5 0.125 No Hit GGGAGATATATGGGAAGATTGAGAGCAGTAAGAGGGAGCAGATGATGGAA 5 0.125 No Hit ATTTGCAACTGAACTGGTGAGGTTTTTATGGCCCATTACTCATATCTATG 5 0.125 No Hit TGAAGCAGAAGAGTACGACAATGAGGAGAAACATGACTACGAGGTAAACC 5 0.125 No Hit CTTCTCATAAGCCAAAATCGCCTTTTGAGGCTGGCTTAATCTTTGGTACA 5 0.125 No Hit CCGTAGATTTCATCTTCTCCTCCTCTCACTCTACCTCTCTCTTCCTTTTC 5 0.125 No Hit AGACCAAGAAATAAACAACAAGCAATCAGATTTTTCACCCAGGAAACAAA 5 0.125 No Hit CTCCCATGCTACTGCAAACCACAAGTTATAAGGCACATTTTATACAAGTG 5 0.125 No Hit CTAGGCTGAGACTTGGTCATCCTTGCTTCCCCGTAGGGTTCATCGTTATC 5 0.125 No Hit CTCTGAGGAGACTTCACCATCAAAGTGCTCCGGTGACTTGTCAGAAGCCA 5 0.125 No Hit CCAAGAAAAAGAGTACATGAATTTCCACAACATTTCATCCTCAAGTAAGA 5 0.125 No Hit ATTGGATTGTCACAAAGGCTAAAATACAGTTCCCTTTTGGATGAAACAGA 5 0.125 No Hit CTGGCCTTCCACCGGGACCATCTTCTCTACCTCTTCCTCTTCCACGCCCC 5 0.125 No Hit ATCGGCAGTTTTTGCATCAAAATTATCGTTAAGGAAGTCTCCGTAAGCTT 5 0.125 No Hit TTCATCTAGGGAAACAACACTGAAGTTAGATACCGATGCAGCGACTTTGC 5 0.125 No Hit CTTCGATCGAGTTCTTCATATATATGATCGACTAAGGACGGTGATGATCA 5 0.125 No Hit GGTTTTTTGAAGCAGATTCTGCGGATCTCTTCTTGCCAACTTCAGCCTTC 5 0.125 No Hit GTCTATCAAACTCGCTTGCATGGACTGCTGTGGCATAACCAGCAGCAGCC 5 0.125 No Hit CTCATTGTCATGCTTGAAGTTAGTGTGTAAGTTGCAGTTACAGAAAGATC 5 0.125 No Hit AGTGACCAGACTTCTTCTTTTTATTTATTATAGTTCCATAAAACTGCTTG 5 0.125 No Hit CCTCTCTTGAACCTGGTTGAGAACATCAGAGTCGGAAGCTGATGATACAA 5 0.125 No Hit CTAACTTGAGAGTGTCAATGGGGCAAGTCTCTTGCTTCGGTGGTGTTGGT 5 0.125 No Hit AAATTAAATCAACAGCAAACACTTAATGAAATAAAAATAACAGTATTAAT 5 0.125 No Hit TGCTGAACTTGGAAATATCTAGTGATCGCCCGTAGGACCCTGACTTGTGA 5 0.125 No Hit CTGCAAAATAATGGGCATGTTAGAGGTGTAGAACAGCTTGATTGGATATG 5 0.125 No Hit AGCTCCCAAAATTATCCAAAACCCCCACGAGAGGAGGCAGTGAGAAAAAT 5 0.125 No Hit GAGCCTTGTGAACCATAAGCATGCATAGAATCCGATTGTCCCCGTTAGCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.2125 0.0 0.0 0.0 0.0 76-77 0.3125 0.0 0.0 0.0 0.0 78-79 0.3375 0.0 0.0 0.0 0.0 80-81 0.425 0.0 0.0 0.0 0.0 82-83 0.44999999999999996 0.0 0.0 0.0 0.0 84-85 0.5 0.0 0.0 0.0 0.0 86-87 0.5875 0.0 0.0 0.0 0.0 88-89 0.7749999999999999 0.0 0.0 0.0 0.0 90-91 1.125 0.0 0.0 0.0 0.0 92-93 1.275 0.0 0.0 0.0 0.0 94-95 1.4375 0.0 0.0 0.0 0.0 96-97 1.5625 0.0 0.0 0.0 0.0 98-99 1.6875 0.0 0.0 0.0 0.0 100-101 1.8875000000000002 0.0 0.0 0.0 0.0 102-103 2.2625 0.0 0.0 0.0 0.0 104-105 2.4625 0.0 0.0 0.0 0.0 106-107 2.8 0.0 0.0 0.0 0.0 108-109 3.35 0.0 0.0 0.0 0.0 110-111 3.7125 0.0 0.0 0.0 0.0 112-113 4.125 0.0 0.0 0.0 0.0 114-115 4.375 0.0 0.0 0.0 0.0 116-117 4.8875 0.0 0.0 0.0 0.0 118-119 5.4 0.0 0.0 0.0 0.0 120-121 6.112500000000001 0.0 0.0 0.0 0.0 122-123 6.65 0.0 0.0 0.0 0.0 124-125 6.975 0.0 0.0 0.0 0.0 126-127 7.5 0.0 0.0 0.0 0.0 128-129 8.0375 0.0 0.0 0.0 0.0 130-131 8.5375 0.0 0.0 0.0 0.0 132-133 9.1875 0.0 0.0 0.0 0.0 134-135 10.024999999999999 0.0 0.0 0.0 0.0 136-137 10.6125 0.0 0.0 0.0 0.0 138-139 11.55 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCCAGT 10 0.006830828 145.0 2 CATTGCG 10 0.006830828 145.0 6 GGAAGCA 10 0.006830828 145.0 1 GCATTGC 10 0.006830828 145.0 5 AAGCATT 10 0.006830828 145.0 3 >>END_MODULE SRR26075400 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075400_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2835 37.0 37.0 37.0 37.0 37.0 2 36.425 37.0 37.0 37.0 37.0 37.0 3 36.409 37.0 37.0 37.0 37.0 37.0 4 36.5105 37.0 37.0 37.0 37.0 37.0 5 36.4655 37.0 37.0 37.0 37.0 37.0 6 36.4935 37.0 37.0 37.0 37.0 37.0 7 36.43 37.0 37.0 37.0 37.0 37.0 8 36.421 37.0 37.0 37.0 37.0 37.0 9 36.5165 37.0 37.0 37.0 37.0 37.0 10-14 36.4298 37.0 37.0 37.0 37.0 37.0 15-19 36.42040000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.3899 37.0 37.0 37.0 37.0 37.0 25-29 36.256800000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.188 37.0 37.0 37.0 37.0 37.0 35-39 36.0984 37.0 37.0 37.0 37.0 37.0 40-44 36.084999999999994 37.0 37.0 37.0 37.0 37.0 45-49 36.0311 37.0 37.0 37.0 37.0 37.0 50-54 35.923500000000004 37.0 37.0 37.0 37.0 37.0 55-59 35.985 37.0 37.0 37.0 37.0 37.0 60-64 35.9958 37.0 37.0 37.0 37.0 37.0 65-69 35.909 37.0 37.0 37.0 37.0 37.0 70-74 35.8793 37.0 37.0 37.0 37.0 37.0 75-79 35.81600000000001 37.0 37.0 37.0 37.0 37.0 80-84 35.852999999999994 37.0 37.0 37.0 37.0 37.0 85-89 35.7492 37.0 37.0 37.0 37.0 37.0 90-94 35.65239999999999 37.0 37.0 37.0 37.0 37.0 95-99 35.723600000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.6064 37.0 37.0 37.0 37.0 37.0 105-109 35.5416 37.0 37.0 37.0 37.0 37.0 110-114 35.5258 37.0 37.0 37.0 37.0 37.0 115-119 35.4875 37.0 37.0 37.0 37.0 37.0 120-124 35.402300000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.4133 37.0 37.0 37.0 37.0 37.0 130-134 35.4067 37.0 37.0 37.0 34.6 37.0 135-139 35.2103 37.0 37.0 37.0 27.4 37.0 140-144 35.177499999999995 37.0 37.0 37.0 27.4 37.0 145-149 35.099900000000005 37.0 37.0 37.0 25.0 37.0 150-151 34.8725 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 3.0 15 2.0 16 3.0 17 7.0 18 7.0 19 3.0 20 6.0 21 5.0 22 4.0 23 7.0 24 10.0 25 3.0 26 13.0 27 9.0 28 17.0 29 15.0 30 16.0 31 28.0 32 56.0 33 83.0 34 188.0 35 591.0 36 2711.0 37 213.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.275 20.4 11.575000000000001 27.750000000000004 2 27.6 24.8 30.099999999999998 17.5 3 21.6 28.1 30.125 20.175 4 25.224999999999998 33.75 21.45 19.575 5 25.874999999999996 33.6 22.025 18.5 6 21.575 40.925 21.175 16.325 7 21.925 22.85 36.675000000000004 18.55 8 22.75 25.95 28.075 23.225 9 23.175 24.9 29.575000000000003 22.35 10-14 23.84 29.775000000000002 25.03 21.355 15-19 24.625 27.860000000000003 26.25 21.265 20-24 24.525 27.825 25.900000000000002 21.75 25-29 24.705 27.400000000000002 26.605 21.29 30-34 24.305 28.04 27.075 20.580000000000002 35-39 23.97 28.444999999999997 26.36 21.224999999999998 40-44 25.305 28.15 25.724999999999998 20.82 45-49 24.175 27.905 26.44 21.48 50-54 23.445 28.110000000000003 27.715 20.73 55-59 24.48 27.884999999999998 26.295 21.34 60-64 24.45 27.54 26.58 21.43 65-69 24.335 28.299999999999997 27.1 20.265 70-74 24.495 28.1 26.87 20.535 75-79 24.14 28.194999999999997 26.919999999999998 20.745 80-84 24.94 27.495000000000005 26.565 21.0 85-89 24.935 27.955000000000002 26.115 20.995 90-94 24.485 27.21 26.91 21.395 95-99 25.285000000000004 27.515 26.490000000000002 20.71 100-104 25.235000000000003 28.845 25.71 20.21 105-109 25.295 28.74 25.41 20.555 110-114 24.605 29.160000000000004 25.724999999999998 20.51 115-119 24.45 28.265 26.72 20.565 120-124 24.535 27.255000000000003 27.065 21.145 125-129 26.075 28.27 25.995 19.66 130-134 25.595000000000002 27.57 26.805 20.03 135-139 25.85 28.615000000000002 26.38 19.155 140-144 25.757575757575758 27.86278627862786 26.17761776177618 20.2020202020202 145-149 26.317895368610582 28.05841752525758 26.537961388416527 19.085725717715317 150-151 27.46936734183546 26.19404851212803 26.30657664416104 20.030007501875467 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.5 15 1.5 16 1.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.5 25 0.5 26 0.5 27 4.0 28 4.0 29 2.0 30 4.0 31 9.0 32 16.5 33 22.0 34 22.0 35 38.0 36 63.0 37 71.0 38 94.0 39 129.5 40 176.0 41 232.5 42 263.5 43 284.5 44 298.5 45 288.5 46 268.5 47 243.0 48 239.0 49 232.0 50 173.5 51 129.0 52 136.5 53 129.5 54 89.5 55 64.5 56 54.5 57 47.0 58 32.5 59 18.5 60 16.0 61 18.5 62 17.0 63 9.5 64 5.0 65 3.5 66 2.5 67 1.5 68 1.5 69 4.5 70 4.5 71 1.5 72 1.5 73 1.0 74 0.5 75 0.0 76 1.5 77 2.0 78 1.0 79 0.5 80 1.0 81 3.0 82 2.0 83 0.0 84 0.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.5 96 1.0 97 1.0 98 1.0 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.01 145-149 0.03 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.925 #Duplication Level Percentage of deduplicated Percentage of total 1 64.05416495691424 39.025 2 20.96840377513336 25.55 3 8.124743537135823 14.85 4 3.816167418957735 9.3 5 1.600328272466147 4.875 6 0.7796471070988921 2.85 7 0.24620434961017645 1.05 8 0.16413623307345096 0.8 9 0.04103405826836274 0.22499999999999998 >10 0.2051702913418137 1.4749999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 14 0.35000000000000003 No Hit AACCCTCTCCGTCTACTGAAGTCAGCTCTACAGCCAAGATAAGGAGTGAA 14 0.35000000000000003 No Hit GGCTAGATCCGGTGGTTTCTCCGGGAGCAGTTTCGACCCATGCGCATTCT 11 0.27499999999999997 No Hit CTATCGAGAAGGTTGGATCTGGAAGCGGCAAGACTTCCAAGCCTGTTGCT 10 0.25 No Hit GAAGGAGTTTACACCACCAGCACTTGTGGCAGTACTCCCATGGATGTGAA 10 0.25 No Hit TGGCTGGACCCTGCCGCACCACGAGGCGCTGTCTGCGAGTCGGGTTGTTT 9 0.22499999999999998 No Hit ATTTTCTGACATCAGCATTCTGGAGAATCAAGAACATTTTGTTTATCCAT 8 0.2 No Hit CAATCCAGTGGTGCCTATTTCAGCTCGAATGAATGTTACTGGAGCAAATG 8 0.2 No Hit GAAGTTTTCTGGCTTCCCATCACCAAATCCTGGTATAATGCTGCCACTGA 8 0.2 No Hit GAAATTCTGGAGACAAAGCTGTGCATTCCGAAGTCACGAATCTTCCTTAA 8 0.2 No Hit GTCAAATTTCTTGTATTTGTTAAACTTGTATCAGTCGTCAAAGTTCAAAT 7 0.17500000000000002 No Hit GGGGTACTCTCTTCCATCTCCATCACATTCTGATGATGGGACAGATGACA 7 0.17500000000000002 No Hit GGAAAGCCCAGAAAAAGTCTGAAAGAGAGCTTGAACTGAAAGGGCGATTT 7 0.17500000000000002 No Hit GAAAACAATGGCACTACATGGAAAGATTGAGACAACATTAGAACTCAAGT 7 0.17500000000000002 No Hit CTTTTTCCCTCATCTCTCTCTCTCTCACCTGACCGACACTGTGGAGGAGA 7 0.17500000000000002 No Hit GGGCAAGCTCCCGGGGGGTGGCCGTGCTAGTGGTGTCCGTGGTGAAGGAG 7 0.17500000000000002 No Hit CCTGATTCTAAGCAGAAGGTGAAGATTGTGGAACCGAACAAAGATGGGAA 6 0.15 No Hit ATTCTCTTCGAATTCTTCACTTTTAGTGAAAATCCCCATCCGGGCATCCT 6 0.15 No Hit ATTGGAAGTTATGGATAATGTCTTGCCTTCGAAATATGAGGAAGCAAGAG 6 0.15 No Hit GGTGGTCAGACAGAAGTGGTAGCCATTGACTTTGCCAAGGTTGGTTCTTC 6 0.15 No Hit GCCTTAGCATCTTCACTTCCCAAGCTCGCTTTCTGAAAAGTGAAAGGAGA 6 0.15 No Hit CTCTAATGATCAACATGGGTTCAATGGTCTTGGCGGGTACGGTTACGGAT 6 0.15 No Hit CTTCAAGGAATCACATAAAACAATTACCGTCTCTCTTCCCTTGGTTTTCT 6 0.15 No Hit GGACTTGATAAGGGACAGAAATATTCAAATGCTGTCAGATTCTTGCTTGG 6 0.15 No Hit AGGTGGTGCAGACCGCAATGGGCGTGGTGGCCCTGGTAGGCCTGCCTATT 6 0.15 No Hit ATCTGCGAACAGAATTTCGAGTTCGGGAAAATTCTGATAGGAAGCAATCT 6 0.15 No Hit GGTACCTATGTTGTTCTTGCTGGCTTGTCGTTGGGACTTAATGGAAAATT 6 0.15 No Hit AAGAAAAGCAGCTATGACTGTTGAGATGGGGCACTTCGTTGAGCAGTTTC 6 0.15 No Hit GCAATTTCAACGAGGAAACATACACGAAGAAAGGATTTGAATGGGTGAAT 6 0.15 No Hit CAAATCCTGATGACCCTCTTGTACCAGAGATTGCCCATATGTACAAGACT 6 0.15 No Hit ATTTTCTCTTAATCATGAAATTTACTTGCTTGAGCAAAGGAAGTGGTTTC 6 0.15 No Hit CATAGATAAAATGCGCAGTGAACTGAGGTATGAAATTGATAAAGTAACTG 6 0.15 No Hit AGCGGAAACATGGAAGTGGCTCAGGAAGATTGGGACTTGGGGCAGCAGCC 6 0.15 No Hit CAATAAACGTGCAAAAGATATTCAAGATCGTGCTCGCGAAGGCCTTTGAT 6 0.15 No Hit AGAATTTAGACAAGCGAGGTTAGCCAAACAACCGCAGGTTCAGCTTAGCG 6 0.15 No Hit GACGAAATATCCCTAGGGCTAAATCATATGACGAGGGAAAGGACTCAGCT 5 0.125 No Hit AGAACAAACCAGTCACCAACAGCAGAGACACTCAAGAGGTCAAGTTAGAG 5 0.125 No Hit ATGGGATGGGCCATGGAACTACATCCTATAATGCTGTTGAATTCTCGAAA 5 0.125 No Hit GTCACAGGAAGAAAGGTTGATGCGCTACAAGGGTTTCAAGGAGGGTCATA 5 0.125 No Hit ACAGAATCCACAGGATGCTGAAGCTGGGCTTGAGCATCGATGAGGATGCT 5 0.125 No Hit GTGCTACTGGAAGTGATATTCCACTTAATTCAGACATGACAGCTTCAAGT 5 0.125 No Hit CTAAAATCCAAGATAAAGAGGGCATCCCTCCAGACCAACAGAGGCTCATC 5 0.125 No Hit GATATACTTACAGCGAGCAGAAAGGATTTGATTCTTGCTTACGAAAATGG 5 0.125 No Hit CACCCTAAGCCACCAGTAAAACCTCCCAAAGTAAAGCCGCCACCAATCGT 5 0.125 No Hit TCCAGATTTAGATTTGTAAGTAGAGCAGGTTAAGACGCTAGTCTTTAGTT 5 0.125 No Hit GTCCTGATCACACATCCCTTTTCTCGTTCTATCTCGATCTTCTAGTTCCA 5 0.125 No Hit TCTCGGTCTGAGGAGCCAAGGACACGAGAAGATCGGCAAACTGAACGGGA 5 0.125 No Hit GGAATATTTGCATTCAAAGAACATTGTGCATTTTGATTTGAAATGTGACA 5 0.125 No Hit AAAAGGAAAAAAAGAAAAAGAAAAAACCAAGCTTTCATATGGAAGTAGGC 5 0.125 No Hit CAATGTTCCAATCAATTTAACAGTTTTTGACAAAGACACGTTCACTGTGG 5 0.125 No Hit ATCACAACAGTGCCGAAGATAAAGAAGAAGAAGGCGGGTTCGATCACTCA 5 0.125 No Hit ATGATAATGACCAGCCTAACCAAGGTCCTTCTTCCTATCGTGAAGAGAGA 5 0.125 No Hit TCTACTTCCAAGGGTTCCGTGTGGTTTTACCTGTGAGGTCAAAGAATGCT 5 0.125 No Hit AACTGAACCCACCAAAGGGTTTTATCATCTCCCAAGATCTATCATGGCTA 5 0.125 No Hit GAAAGGGAAAGAAAAATAAGAAAGGAAGACGAAGAATCCTCTCGGAGACG 5 0.125 No Hit GTAATAACTAGCGTCCATTTTTCACAGAGAGGCGCTTAGAGAGAGATATC 5 0.125 No Hit GTTAAATAAAAAGAAGCTCTGCGCTTGTCAGGGCTATAAGAAGAAGAAAA 5 0.125 No Hit CTCCTCTTATTTTGGCAATCTCCGCCTGCGCCCTATCCAAAATTTGCTTC 5 0.125 No Hit GCAAGATTGAGAAGAAGACCCGCATTCATGACACAACTCCGGGGACCCTC 5 0.125 No Hit TGACGGTGACAAGGTTTCCCCCTAATCGAGACGCTGCAATAACACAGGGG 5 0.125 No Hit GTGGTGATGGATTGATGTGGCATGCGGATTTGAAACAACATGCTTCTGGT 5 0.125 No Hit GATCAATTCAACACACAAATCCGATCGACAAGCGAATCCTTTCTTTCTCC 5 0.125 No Hit CTTACTGATGATCAGTGGATGAAAGTTGAGGTTGCTTTAAGGGATCTCAT 5 0.125 No Hit TGTTGCTGGACCTCAGCCAGAACGTGGCCTTGGCAAGCTTAGAAAGATCA 5 0.125 No Hit ATTTTGTTTTCTTTCTGCAGGGTTTCTGGTTGCTAGTGAGAGTAAGCATA 5 0.125 No Hit GTGATCACGAGTGTGATCCAGAAGAATATGGTGCCTGTGACTCGGGCTGC 5 0.125 No Hit GCAAGGCTGATCCCAGTTACTGGGACAAAATCTCCCAGGGAGGCCTGCAG 5 0.125 No Hit CAGCACTGTACCTCTTCCTCTATGCAACATTCTACTTCTTCACAAAGCTT 5 0.125 No Hit CCGTTGGCAATCTTGATCCTCTATACATTGTAAATATGGTCCCCAATGGC 5 0.125 No Hit AGAAACACTATGCGGTGGTTCCTGAGTCTTTCTATTGATGTGTATCGGAA 5 0.125 No Hit GTTGCATTTATCTAAAGTATTCTCACTTTACTTAACACTTGAGCTACATA 5 0.125 No Hit CCACCCGGTCCACCACAGCGCGAACCTCCTCCCCCTCGTGGCCATTCTCC 5 0.125 No Hit TGAGAATGGCTGCAAATGTGGATCAAACTGCACCTGTGATCCATGCTCCT 5 0.125 No Hit CCTCGACCTGGATCCAGTTATGGTGGGCCTCAACCGGCTTATGGATTGCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.225 0.0 0.0 0.0 0.0 76-77 0.3625 0.0 0.0 0.0 0.0 78-79 0.3875 0.0 0.0 0.0 0.0 80-81 0.475 0.0 0.0 0.0 0.0 82-83 0.5 0.0 0.0 0.0 0.0 84-85 0.55 0.0 0.0 0.0 0.0 86-87 0.6375 0.0 0.0 0.0 0.0 88-89 0.825 0.0 0.0 0.0 0.0 90-91 1.1749999999999998 0.0 0.0 0.0 0.0 92-93 1.325 0.0 0.0 0.0 0.0 94-95 1.4874999999999998 0.0 0.0 0.0 0.0 96-97 1.65 0.0 0.0 0.0 0.0 98-99 1.7875 0.0 0.0 0.0 0.0 100-101 2.0 0.0 0.0 0.0 0.0 102-103 2.3875 0.0 0.0 0.0 0.0 104-105 2.5875 0.0 0.0 0.0 0.0 106-107 2.925 0.0 0.0 0.0 0.0 108-109 3.45 0.0 0.0 0.0 0.0 110-111 3.7875 0.0 0.0 0.0 0.0 112-113 4.1625 0.0 0.0 0.0 0.0 114-115 4.4 0.0 0.0 0.0 0.0 116-117 4.95 0.0 0.0 0.0 0.0 118-119 5.5 0.0 0.0 0.0 0.0 120-121 6.2875 0.0 0.0 0.0 0.0 122-123 6.8125 0.0 0.0 0.0 0.0 124-125 7.1125 0.0 0.0 0.0 0.0 126-127 7.65 0.0 0.0 0.0 0.0 128-129 8.1875 0.0 0.0 0.0 0.0 130-131 8.7 0.0 0.0 0.0 0.0 132-133 9.4125 0.0 0.0 0.0 0.0 134-135 10.4625 0.0 0.0 0.0 0.0 136-137 11.1375 0.0 0.0 0.0 0.0 138-139 12.1125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAGTGC 10 0.006830828 145.0 7 AGTGCCG 10 0.006830828 145.0 9 CAGTGCC 10 0.006830828 145.0 8 >>END_MODULE Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159695 spots for SRR26075400.sra Written 3159695 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra Read 3159691 spots for SRR26075400.sra Written 3159691 spots for SRR26075400.sra SRR ids: ['SRR26075400.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ag2rcdml SRR26075400.sra spots: 63193824 blocks: [[1, 3159691], [3159692, 6319382], [6319383, 9479073], [9479074, 12638764], [12638765, 15798455], [15798456, 18958146], [18958147, 22117837], [22117838, 25277528], [25277529, 28437219], [28437220, 31596910], [31596911, 34756601], [34756602, 37916292], [37916293, 41075983], [41075984, 44235674], [44235675, 47395365], [47395366, 50555056], [50555057, 53714747], [53714748, 56874438], [56874439, 60034129], [60034130, 63193824]] SRR26075400 file size 23345289 SRR26075400 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075400 SRR26075400_1.fastq SRR26075400_2.fastq Input file: SRR26075400_1.fastq Paired file: SRR26075400_2.fastq trimmed: SRR26075400-trimmed-pair1.fastq, SRR26075400-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:05:52 2025 >> started Wed Feb 12 01:07:50 2025 >> done (117.757s) 63193824 read pairs processed; of these: 300 ( 0.00%) short read pairs filtered out after trimming by size control 69957 ( 0.11%) empty read pairs filtered out after trimming by size control 63123567 (99.89%) read pairs available; of these: 10075643 (15.96%) trimmed read pairs available after processing 53047924 (84.04%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 21 0.00% 19 38 0.00% 20 27 0.00% 21 22 0.00% 22 32 0.00% 23 40 0.00% 24 56 0.00% 25 41 0.00% 26 75 0.00% 27 59 0.00% 28 60 0.00% 29 48 0.00% 30 58 0.00% 31 74 0.00% 32 73 0.00% 33 92 0.00% 34 100 0.00% 35 84 0.00% 36 86 0.00% 37 95 0.00% 38 120 0.00% 39 97 0.00% 40 152 0.00% 41 127 0.00% 42 149 0.00% 43 143 0.00% 44 191 0.00% 45 162 0.00% 46 215 0.00% 47 207 0.00% 48 255 0.00% 49 260 0.00% 50 284 0.00% 51 342 0.00% 52 406 0.00% 53 415 0.00% 54 464 0.00% 55 544 0.00% 56 618 0.00% 57 838 0.00% 58 886 0.00% 59 1013 0.00% 60 1148 0.00% 61 1396 0.00% 62 1531 0.00% 63 1729 0.00% 64 1956 0.00% 65 2310 0.00% 66 2603 0.00% 67 2877 0.00% 68 3293 0.01% 69 3933 0.01% 70 4393 0.01% 71 5101 0.01% 72 6116 0.01% 73 7031 0.01% 74 8393 0.01% 75 9098 0.01% 76 10688 0.02% 77 11568 0.02% 78 12744 0.02% 79 14173 0.02% 80 15550 0.02% 81 17428 0.03% 82 20013 0.03% 83 22828 0.04% 84 25328 0.04% 85 27914 0.04% 86 30496 0.05% 87 32563 0.05% 88 35408 0.06% 89 37907 0.06% 90 40219 0.06% 91 44039 0.07% 92 46367 0.07% 93 50510 0.08% 94 55005 0.09% 95 59734 0.09% 96 63620 0.10% 97 67738 0.11% 98 70000 0.11% 99 73055 0.12% 100 76219 0.12% 101 79767 0.13% 102 84319 0.13% 103 88897 0.14% 104 94165 0.15% 105 98514 0.16% 106 103870 0.16% 107 106689 0.17% 108 110686 0.18% 109 115070 0.18% 110 115549 0.18% 111 119916 0.19% 112 123659 0.20% 113 127364 0.20% 114 131421 0.21% 115 140423 0.22% 116 143765 0.23% 117 148549 0.24% 118 153554 0.24% 119 155799 0.25% 120 159778 0.25% 121 162688 0.26% 122 165126 0.26% 123 170090 0.27% 124 174812 0.28% 125 179320 0.28% 126 184345 0.29% 127 188862 0.30% 128 194836 0.31% 129 198306 0.31% 130 203584 0.32% 131 204674 0.32% 132 207025 0.33% 133 211559 0.34% 134 212235 0.34% 135 219073 0.35% 136 223771 0.35% 137 227070 0.36% 138 232588 0.37% 139 238687 0.38% 140 242723 0.38% 141 245789 0.39% 142 249762 0.40% 143 248344 0.39% 144 254760 0.40% 145 257626 0.41% 146 260440 0.41% 147 265252 0.42% 148 269055 0.43% 149 272019 0.43% 150 276377 0.44% 151 53047924 84.04% 63123567 reads passed initial QC criterion=sequence-density sequence-density=0.50 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=26 prefix-density=0.50 prefix-fanout=2.0 sequence=TACAGTCCCCAACAAGTTCTCTCCATTCTCTGGATAATTGAAATCTCCAAATACCAGTTTACCTTTCTCCGATCCGAATTTCTTCCAGTTCCAAACTCCTGTGAAGGCAACAGCCTGCGGCACAGAAACATCACCTGGAACCATTGTTTTCTTATCCAAGCTTCCAGCATTCCCAAAATAGATGACTCCGTGAATGCTGAATCTAGCTAAAAGGATTTGCAAAGTTGTTGCCATATTTACTGAA criterion=fanout-score sequence-density=0.04 sequence-density-rank=26 fanout-score=43.17 fanout-score-rank=1 prefix-density=0.23 prefix-fanout=6.9 sequence=CATCATCACCACCATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCA criterion=sequence-density sequence-density=0.47 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=26 prefix-density=0.47 prefix-fanout=2.0 sequence=AAGCGCTCAAGGATATGAAGTTAAGAAAATGCTATTC criterion=fanout-score sequence-density=0.04 sequence-density-rank=24 fanout-score=19.92 fanout-score-rank=1 prefix-density=0.12 prefix-fanout=6.1 sequence=CTTTGTCTGTCTTTGGGTCGATCCGAAAGAGAGGAGCTCTTCTGCGCAATCATGTTGGTCTATCAAGATCTTCTCTCTGGTGATGAGCTTCTCTCGGATTCGTTCCCATACAAGGAGATTGAGAATGGGATACTGTGGGAAG SRR26075400 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:08:35 Started mapping on | Feb 12 01:08:36 Finished on | Feb 12 01:21:37 Mapping speed, Million of reads per hour | 290.97 Number of input reads | 63123567 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 56696210 Uniquely mapped reads % | 89.82% Average mapped length | 292.70 Number of splices: Total | 53904475 Number of splices: Annotated (sjdb) | 52674245 Number of splices: GT/AG | 52890571 Number of splices: GC/AG | 813836 Number of splices: AT/AC | 51448 Number of splices: Non-canonical | 148620 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 3.17 Insertion rate per base | 0.02% Insertion average length | 2.59 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1549354 % of reads mapped to multiple loci | 2.45% Number of reads mapped to too many loci | 173529 % of reads mapped to too many loci | 0.27% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.27% % of reads unmapped: other | 0.19% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4878003 4878003 4878003 N_multimapping 1549354 1549354 1549354 N_noFeature 1355649 56142556 1663200 N_ambiguous 553870 3957 304798 UnstrandedReadsAssigned:54786691 PositiveStrandReadsAssigned:549697 NegativeStrandReadsAssigned:54728212 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075400 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075400-trimmed-pair1.fastq SRR26075400-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 63,123,567 reads, 55,223,618 reads pseudoaligned [quant] estimated average fragment length: 213.336 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,186 rounds 52401 SRR26075400.ke.tsv 34699 SRR26075400.se.tsv 87100 total ==> SRR26075400.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1805.66 5254 49.0028 Potri.005G024800.1.v4.1 1035 822.664 3569.06 73.0632 Potri.004G059700.1.v4.1 961 748.675 8 0.179955 Potri.007G009000.2.v4.1 1416 1203.66 0 0 Potri.003G141000.2.v4.1 2943 2730.66 2076.76 12.8081 Potri.016G087400.1.v4.1 270 90.604 4150.09 771.397 Potri.015G069301.1.v4.1 564 354.089 0 0 Potri.010G195200.1.v4.1 1773 1560.66 1411.71 15.2337 Potri.012G127500.1.v4.1 977 764.669 32971 726.15 ==> SRR26075400.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 158 Potri.001G233950.v4.1 3 Potri.001G122700.v4.1 580 Potri.001G212900.v4.1 16 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 7 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 3772 SRR26075400 completed mapping pipeline successfully