Starting /dee2/code/volunteer_pipeline.sh SRR26075401 current disk space = 3051217920000 free memory = 1443479536 SRR26075401 SRAfilesize 4911fc4fbac01ed6b6a7965d6b9776e0 SRR26075401.sra SRR26075401.sra file validated SRR26075401 is paired end SRR26075401 is conventional basespace SRR26075401 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075401_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.59975 37.0 37.0 37.0 37.0 37.0 2 36.5975 37.0 37.0 37.0 37.0 37.0 3 36.668 37.0 37.0 37.0 37.0 37.0 4 36.693 37.0 37.0 37.0 37.0 37.0 5 36.7115 37.0 37.0 37.0 37.0 37.0 6 36.681 37.0 37.0 37.0 37.0 37.0 7 36.62 37.0 37.0 37.0 37.0 37.0 8 36.735 37.0 37.0 37.0 37.0 37.0 9 36.575 37.0 37.0 37.0 37.0 37.0 10-14 36.6556 37.0 37.0 37.0 37.0 37.0 15-19 36.6092 37.0 37.0 37.0 37.0 37.0 20-24 36.52290000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.470600000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.4924 37.0 37.0 37.0 37.0 37.0 35-39 36.41519999999999 37.0 37.0 37.0 37.0 37.0 40-44 36.3978 37.0 37.0 37.0 37.0 37.0 45-49 36.3591 37.0 37.0 37.0 37.0 37.0 50-54 36.2482 37.0 37.0 37.0 37.0 37.0 55-59 36.2193 37.0 37.0 37.0 37.0 37.0 60-64 36.139300000000006 37.0 37.0 37.0 37.0 37.0 65-69 36.0265 37.0 37.0 37.0 37.0 37.0 70-74 36.108000000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.0303 37.0 37.0 37.0 37.0 37.0 80-84 36.0064 37.0 37.0 37.0 37.0 37.0 85-89 35.8618 37.0 37.0 37.0 37.0 37.0 90-94 35.8363 37.0 37.0 37.0 37.0 37.0 95-99 35.9511 37.0 37.0 37.0 37.0 37.0 100-104 35.833299999999994 37.0 37.0 37.0 37.0 37.0 105-109 35.7454 37.0 37.0 37.0 37.0 37.0 110-114 35.6044 37.0 37.0 37.0 37.0 37.0 115-119 35.5214 37.0 37.0 37.0 37.0 37.0 120-124 35.5975 37.0 37.0 37.0 37.0 37.0 125-129 35.3758 37.0 37.0 37.0 37.0 37.0 130-134 35.3255 37.0 37.0 37.0 32.2 37.0 135-139 35.227 37.0 37.0 37.0 29.8 37.0 140-144 35.1496 37.0 37.0 37.0 27.4 37.0 145-149 35.1374 37.0 37.0 37.0 27.4 37.0 150-151 35.05925 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 0.0 23 5.0 24 7.0 25 4.0 26 12.0 27 15.0 28 27.0 29 17.0 30 24.0 31 49.0 32 67.0 33 108.0 34 139.0 35 439.0 36 2902.0 37 184.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.00125156445557 16.245306633291616 6.958698372966207 35.794743429286605 2 21.05 15.475 32.625 30.85 3 18.15 17.925 26.575 37.35 4 21.45 22.225 26.924999999999997 29.4 5 26.224999999999998 29.175 25.1 19.5 6 22.725 31.4 23.125 22.75 7 15.225 28.525 39.550000000000004 16.7 8 19.650000000000002 26.825 31.65 21.875 9 18.25 24.025 35.5 22.225 10-14 19.936993699369935 29.257925792579258 28.212821282128214 22.592259225922593 15-19 21.015 27.755000000000003 27.265 23.965 20-24 20.685000000000002 28.155 27.884999999999998 23.275000000000002 25-29 21.985 27.834999999999997 27.025 23.155 30-34 21.445 26.76 28.51 23.285 35-39 20.685000000000002 27.76 27.76 23.794999999999998 40-44 21.035 28.075 26.895000000000003 23.995 45-49 20.215 27.375 28.005000000000003 24.404999999999998 50-54 20.455000000000002 27.900000000000002 27.395000000000003 24.25 55-59 21.07 27.46 28.07 23.400000000000002 60-64 21.115000000000002 27.04 28.355000000000004 23.49 65-69 20.39 27.565 27.74 24.305 70-74 21.265 27.505000000000003 27.625 23.605 75-79 20.580000000000002 27.485 28.355000000000004 23.580000000000002 80-84 20.625 27.35 27.79 24.235 85-89 20.51 27.1 27.744999999999997 24.645 90-94 20.75 27.71 27.034999999999997 24.505 95-99 21.16 28.199999999999996 26.465 24.175 100-104 20.385 28.744999999999997 27.200000000000003 23.669999999999998 105-109 21.145 27.229999999999997 27.229999999999997 24.395 110-114 21.495 27.229999999999997 27.74 23.535 115-119 21.185000000000002 27.875 27.265 23.674999999999997 120-124 21.78 27.634999999999998 27.87 22.715 125-129 21.595 27.13 26.905 24.37 130-134 21.855 27.705000000000002 25.635 24.805 135-139 21.37 28.349999999999998 26.314999999999998 23.965 140-144 22.005 27.185 26.38 24.43 145-149 22.48 26.935 26.8 23.785 150-151 21.5625 26.900000000000002 25.650000000000002 25.887500000000003 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 1.0 18 1.5 19 1.0 20 0.5 21 0.0 22 0.0 23 0.0 24 1.5 25 6.5 26 9.0 27 4.5 28 3.0 29 7.0 30 10.5 31 19.0 32 26.5 33 28.5 34 33.0 35 54.5 36 82.0 37 89.0 38 112.0 39 130.0 40 141.0 41 175.5 42 204.0 43 244.0 44 273.0 45 280.5 46 309.5 47 308.5 48 279.0 49 248.5 50 196.0 51 158.0 52 128.0 53 98.0 54 74.0 55 53.0 56 44.0 57 38.5 58 26.5 59 18.5 60 19.5 61 19.5 62 11.0 63 6.5 64 5.5 65 1.5 66 2.5 67 2.0 68 2.0 69 2.0 70 0.5 71 0.5 72 3.0 73 3.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.125 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.150000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 61.74978867286559 36.525 2 22.062552831783602 26.1 3 8.833474218089602 15.675 4 3.9729501267962806 9.4 5 1.6060862214708367 4.75 6 0.760777683854607 2.7 7 0.5071851225697379 2.1 8 0.33812341504649196 1.6 9 0.0 0.0 >10 0.16906170752324598 1.15 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TAATAGTTTTATTAAGATGATGGGTGACCTGCCATGCAAGCGCACCTGTC 14 0.35000000000000003 No Hit CTGCCATTTTGCTTCGTCTCTAGAGAATGATGGATTGGCCGCATTGCTTT 12 0.3 No Hit GGCTGCAAGGAAGGCGTTGTAGGAGGCTAAGTAGCTAACTCCTGAGTCTG 10 0.25 No Hit CCGGTCAGTGTAAACGAGGCACACAAGTCCAGTCAGTGTAAACGAGGCAC 10 0.25 No Hit CATCCACCTTGTTTCCACAAAGAACAATTGGAATGTTTTCACAGACCCTG 8 0.2 No Hit CTTTGCATTCACCACCGGGAAAACCTTCAGTCTTGCACACACTAGCACAG 8 0.2 No Hit GCGGCTGATAGCTCCCTGCCTCCGGTGAGTGGAGTTGGCAATGGGCGGGT 8 0.2 No Hit CTCCATTCTTCCAAACGCATTAAATTATCCACATCATCCAGAACAATGAA 8 0.2 No Hit AGTGACATTAGCAGCCTTAGTACGGCCATCGTCTGAGTTTTCAATCTCAA 8 0.2 No Hit GGGATTAATAGTCCAGTAAGAATGTTCGTTTTCCAGTTCGTTCGATCCAA 8 0.2 No Hit GCTAATTGATTCAGAGAGGTACTTGAAGTAAGCAGGGGCAAATTTTATAA 8 0.2 No Hit AACTAGGAGTAATTTGTTGTGCTCGAAACAGAAACCAAATAGAGAGATTA 8 0.2 No Hit CTGGGCTTGGAGAAATATCAGCTACAAGAGTACCCATACTGGCAGGTTGT 7 0.17500000000000002 No Hit GGAGAGAAGACGATGGTAGCTAAGAGAAAGCATGCGATAAGAAAGGCCTT 7 0.17500000000000002 No Hit GTGCAGAGGGAGGTGGTTCGAGATGGTTATATCTGGTAGTTTTGATCTGA 7 0.17500000000000002 No Hit CTGCAATGATTGTCTCGGTTGTGGTGCTCTCTGAGAAACCCAAGTCAGGG 7 0.17500000000000002 No Hit CCAGTTTTGAATGGCTGGTGATGATCAGGCTGGTTTTGGGAAGGAAGGCT 7 0.17500000000000002 No Hit GTTCTTTCAGGCCATTTAAGCAAAGGGTAGAAGTCACCATAGTAGTGCCT 7 0.17500000000000002 No Hit CATCAGACCACGAAATTGACCCGAATACAGCACCCGAATCATCCAGCATC 7 0.17500000000000002 No Hit AACCCACATCAGTTCATCTTGATCCCATTCAGCTCTCTTCGATTCACAAT 7 0.17500000000000002 No Hit CACCCAGGAAGCCTCCTGGCTCAAGCACACTAGTTGCCACCATGCCTTTG 7 0.17500000000000002 No Hit CCTTGGATATGCTTGGAAGCATGTTTGGGAACATGGAAGGACTGGCTCCT 7 0.17500000000000002 No Hit ATCTGCTCATCCAACTGAGCTTCAAGTTTCCTTGCCTGTTTGCGTAAAGC 7 0.17500000000000002 No Hit ACCACCACCATCATCCTCTCCCTTTCCTCACAACCTCCTATCATGGCGCT 7 0.17500000000000002 No Hit ATCGTCACCTTCCTCCTCGTCGCTAGAAGAGCTGGAAGAACTGCTGCCTG 6 0.15 No Hit GCCTCTCCGAATATGACATAAGTCTCAGAATTTGGGCTCTTGAAGACATC 6 0.15 No Hit ACGGAAGATTTTCAGTGTTGTTGGATCTGTTTGCAGTTTCGGTGGAAGCC 6 0.15 No Hit CAAGAGTTCAAGAATGACTGTAATTTTACTGGCAGGGCATCCCTCACCTT 6 0.15 No Hit AGTTGCTGGAGTTTGAGAGACATCGTCATCATACCCACCTCCATTCAATA 6 0.15 No Hit CTCAATTTCAGCATAAGCCATGATCTTATATTTACAGTCATCATCCTTTT 6 0.15 No Hit ATTCTGGTTCAGTTCAATAGCAGAAAGGTTCACCAATTTGCACAACTCTG 6 0.15 No Hit AGCCATTTATACATTGTTTCCATAACCTCAGCCTCAGCCCATGGCATACT 6 0.15 No Hit CACATACTTCTGGTAAAACGCCACAAGTGCCTTTGCTATGCTCTGCCTGA 6 0.15 No Hit CCTCTCTTCTTCCTCATCAGAATCCCCACTACCCCTTGAAGAACCCCACC 6 0.15 No Hit TCACTTCTTGCACCGAAAACGAACTATAACATTCCAATATCTTATCTAAC 6 0.15 No Hit CTCCGAAACTGAAAACGAGCTTCTTTTTCATCTTTTAGAAGATAAAATAA 6 0.15 No Hit GACAGGACTCCCGAATCAGGCACTTCATCATCTTCTTCTAATACTCGTCT 6 0.15 No Hit GCCGATGTGAATAGGAAACCGAACCATCCTTTTCTTCTTTTACAATCACC 6 0.15 No Hit CCCTTCCATAAGGCCAGACGAAGCGCACCGGAATTACTCCACTATTATCA 6 0.15 No Hit TGATGATTTAGATGAGGAGTCTTTGCTGTTATTTTCTGCACCCGAAGCCG 6 0.15 No Hit ACTCTCTTTCCCTGGAGCTACTCCACCCGATGATGAACCAGGACGACTGT 6 0.15 No Hit GGCCAATAGATAACAAAATCTCAGCCAAACAAAGAGAATCTAGATGCAAG 6 0.15 No Hit GTCCCACTCCCCAGCCCCATTATAGCCTCCATAGTAATAAGATTGATAAC 5 0.125 No Hit GACGCGACCAAACCCATAGCCACCACCATCTAGTAACAGAACCATATCCT 5 0.125 No Hit CTGTTATGGCTTGGTTGGAGAGGGCTAAAGAAGAGTATGCAAGGGACATT 5 0.125 No Hit GTTGAATATACTTGGAAGAGCATTTATTCGGTCACTCTACAGCCTCCATA 5 0.125 No Hit GTATGGTAGTAAGTAGTATAGTATCGATGATGATAACGATGATGACGGTA 5 0.125 No Hit GGGATTGAATGCCACCACAATACAATGAACCAATTGCAATTAACATTCTA 5 0.125 No Hit GTCGTTATTTGTAAAATAGATTCCAGTAGCATCAACCTGCTCCGTTCACT 5 0.125 No Hit CTCTTATTACTGGCAGCAGATATCAGAGCTCCTGTCAAAGCACCTCCAAG 5 0.125 No Hit TGATAAGCTTCCCCTTTGAGTTTCTGAGAGATTTCAGCACAGTCTTGTAC 5 0.125 No Hit TAATAGTCCAGTAAGAATGTTCGTTTTCCAGTTCGTTCGATCCAAGATCA 5 0.125 No Hit TCTCACTCCGGATGTTCCAATCAAGACTGTTGGTTTGATTGCCTTGACAA 5 0.125 No Hit CCCCGGACCATCATGTGAAGCTTCACTATTTGCCTCTGAGTGATAGAGTA 5 0.125 No Hit TATCATTTCCTTATCGTTATTTCTCAGTGACCTGACACCAAACTTATCTG 5 0.125 No Hit GTGGATAATCATTCTCCTTCATATTGAATGTAGAAGGTTTTTCAGTGGTT 5 0.125 No Hit GGAGCTAGGAAGGTGCTGATGAAGGTTACTGTGGTGGCTGTGATGGCTAG 5 0.125 No Hit ACCATCAATTCCTAGAGTTCAGATGACAAAATAGTCACACACATTCATTG 5 0.125 No Hit GCCTTGCGGAATAAACCAGGTGGGATGTTGGAGATTCTAAGTGTTTCATT 5 0.125 No Hit CTAAAATATAGACCAAACAGAGGATTGAGACACGGATCAGAAGCTCTTGT 5 0.125 No Hit CAGCCAAAACAACAACACCAGTAGTCCCATCACCGATTTCATAATCCTGA 5 0.125 No Hit CCATAATACCAGCAGAAGTTGTGAGCACGATGTATCCAAACTGTCTTGAG 5 0.125 No Hit GTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTT 5 0.125 No Hit TGACAGATCGGTGAGGATGGAGAAGGCTGGTTTGGGGGTCAAAAAATCAA 5 0.125 No Hit CCGTAGGGCTTAGTGATTCCATTCACCCTATTTTCAGATTTTCCCTGCCT 5 0.125 No Hit AGCCTTTGTATCTTTGGAGCTTTACTGACTTTCTTCCCAGATTTTGTTGT 5 0.125 No Hit CTTCATGTTCCACTGATTTTTCAGGGCAATGAGATGCTTCCTTATCAATT 5 0.125 No Hit CTTGGGTTTCTTGATGCCATTTTGGTGAGCTTTGTGTGACTGGTTGTGTG 5 0.125 No Hit CTCTCGACAAGCTTCTCAATAAGATCAGCAGCATCCTGAACTGTTGCAAT 5 0.125 No Hit CTCAAATTGAAAACATAAAATACAACCACTTCTCTGTACACATGTTATTT 5 0.125 No Hit AGGAATTTCAATTGGTTCCTCTGAGCTAGTGTTGCTGCCACCAGATTCAC 5 0.125 No Hit ACAGACTAAGATGTTTCTCTACTGAGCTTGTTGTTTACTCCTAAAGCTTG 5 0.125 No Hit CCTCGCCCCTCCTTTGCCTTCGGCCTCTGCTGCTACCACCACCACCGAAC 5 0.125 No Hit CACCAATAGCTTAATTAAAGCATTAAACATCAAGCACCAACATTAATAAA 5 0.125 No Hit AGCATTCTGTGGATGCGACTGCCAAAGGTGTTGGGATCATCCAGGCTGAA 5 0.125 No Hit ATCATGATACCAATTCTCTCTTTCTCTCTGTTTCCACCACCTCAAGCATC 5 0.125 No Hit GTCTGCTTGTAGAACATCTGTGATGCAGCACTCAGATCAGAACTCTTCTC 5 0.125 No Hit GGAGGAGGAGAATGGCCACGAGGGGGAGGAGGTTCGCGCTGTGGTGGACC 5 0.125 No Hit GTCCATAAAAAGTACAGGAACTGCAGAGGCCCTCTGGAACTGTAGGAGCG 5 0.125 No Hit GAGGTAGTTCAGCCTTGCAAACCAATAACACCACATGCTACTCTGCCGCC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.037500000000000006 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.11249999999999999 0.0 0.0 0.0 0.0 80-81 0.16249999999999998 0.0 0.0 0.0 0.0 82-83 0.1875 0.0 0.0 0.0 0.0 84-85 0.225 0.0 0.0 0.0 0.0 86-87 0.25 0.0 0.0 0.0 0.0 88-89 0.2875 0.0 0.0 0.0 0.0 90-91 0.32499999999999996 0.0 0.0 0.0 0.0 92-93 0.5125 0.0 0.0 0.0 0.0 94-95 0.5874999999999999 0.0 0.0 0.0 0.0 96-97 0.7 0.0 0.0 0.0 0.0 98-99 1.0375 0.0 0.0 0.0 0.0 100-101 1.325 0.0 0.0 0.0 0.0 102-103 1.65 0.0 0.0 0.0 0.0 104-105 1.875 0.0 0.0 0.0 0.0 106-107 2.2249999999999996 0.0 0.0 0.0 0.0 108-109 2.7625 0.0 0.0 0.0 0.0 110-111 3.1624999999999996 0.0 0.0 0.0 0.0 112-113 3.6625 0.0 0.0 0.0 0.0 114-115 4.2 0.0 0.0 0.0 0.0 116-117 4.9125 0.0 0.0 0.0 0.0 118-119 5.3875 0.0 0.0 0.0 0.0 120-121 6.012499999999999 0.0 0.0 0.0 0.0 122-123 6.775 0.0 0.0 0.0 0.0 124-125 7.324999999999999 0.0125 0.0 0.0 0.0 126-127 8.4375 0.025 0.0 0.0 0.0 128-129 9.399999999999999 0.025 0.0 0.0 0.0 130-131 10.662500000000001 0.025 0.0 0.0 0.0 132-133 11.2875 0.025 0.0 0.0 0.0 134-135 12.4 0.025 0.0 0.0 0.0 136-137 13.3625 0.025 0.0 0.0 0.0 138-139 13.9125 0.025 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGATG 10 0.006830828 145.0 8 >>END_MODULE SRR26075401 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075401_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1745 37.0 37.0 37.0 37.0 37.0 2 36.3385 37.0 37.0 37.0 37.0 37.0 3 36.362 37.0 37.0 37.0 37.0 37.0 4 36.383 37.0 37.0 37.0 37.0 37.0 5 36.436 37.0 37.0 37.0 37.0 37.0 6 36.3395 37.0 37.0 37.0 37.0 37.0 7 36.278 37.0 37.0 37.0 37.0 37.0 8 36.382 37.0 37.0 37.0 37.0 37.0 9 36.419 37.0 37.0 37.0 37.0 37.0 10-14 36.3703 37.0 37.0 37.0 37.0 37.0 15-19 36.334399999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.3361 37.0 37.0 37.0 37.0 37.0 25-29 36.224900000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.0974 37.0 37.0 37.0 37.0 37.0 35-39 36.0182 37.0 37.0 37.0 37.0 37.0 40-44 36.028499999999994 37.0 37.0 37.0 37.0 37.0 45-49 35.9353 37.0 37.0 37.0 37.0 37.0 50-54 35.90079999999999 37.0 37.0 37.0 37.0 37.0 55-59 35.945499999999996 37.0 37.0 37.0 37.0 37.0 60-64 35.998599999999996 37.0 37.0 37.0 37.0 37.0 65-69 35.8531 37.0 37.0 37.0 37.0 37.0 70-74 35.798 37.0 37.0 37.0 37.0 37.0 75-79 35.7419 37.0 37.0 37.0 37.0 37.0 80-84 35.7637 37.0 37.0 37.0 37.0 37.0 85-89 35.7254 37.0 37.0 37.0 37.0 37.0 90-94 35.668600000000005 37.0 37.0 37.0 37.0 37.0 95-99 35.7091 37.0 37.0 37.0 37.0 37.0 100-104 35.5162 37.0 37.0 37.0 37.0 37.0 105-109 35.5897 37.0 37.0 37.0 37.0 37.0 110-114 35.4498 37.0 37.0 37.0 37.0 37.0 115-119 35.4895 37.0 37.0 37.0 37.0 37.0 120-124 35.3692 37.0 37.0 37.0 37.0 37.0 125-129 35.340999999999994 37.0 37.0 37.0 37.0 37.0 130-134 35.235 37.0 37.0 37.0 34.6 37.0 135-139 35.0635 37.0 37.0 37.0 25.0 37.0 140-144 35.12389999999999 37.0 37.0 37.0 27.4 37.0 145-149 35.0079 37.0 37.0 37.0 25.0 37.0 150-151 34.7525 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 3.0 15 3.0 16 3.0 17 4.0 18 4.0 19 1.0 20 3.0 21 6.0 22 15.0 23 12.0 24 12.0 25 18.0 26 16.0 27 11.0 28 12.0 29 10.0 30 21.0 31 36.0 32 47.0 33 74.0 34 150.0 35 614.0 36 2691.0 37 231.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.99699849924962 23.461730865432717 8.904452226113056 23.6368184092046 2 28.4 27.725 26.900000000000002 16.975 3 21.125 28.725 29.599999999999998 20.549999999999997 4 26.700000000000003 34.75 20.875 17.675 5 24.099999999999998 37.3 22.475 16.125 6 21.925 38.550000000000004 21.224999999999998 18.3 7 22.75 24.3 33.475 19.475 8 20.5 27.1 26.775 25.624999999999996 9 22.85 27.3 26.5 23.35 10-14 24.625 29.765000000000004 24.725 20.885 15-19 24.535 28.585 26.83 20.05 20-24 24.63 28.74 25.785000000000004 20.845 25-29 24.47 28.82 25.91 20.8 30-34 24.055 28.785 26.584999999999997 20.575 35-39 24.585 28.349999999999998 26.875 20.19 40-44 24.94 28.485 25.919999999999998 20.655 45-49 25.264999999999997 28.27 27.034999999999997 19.43 50-54 24.6 27.625 26.665 21.11 55-59 24.185000000000002 28.375 26.279999999999998 21.16 60-64 25.064999999999998 27.79 27.205000000000002 19.939999999999998 65-69 24.2 27.48 26.479999999999997 21.84 70-74 24.93 28.725 26.224999999999998 20.119999999999997 75-79 24.02 28.07 27.1 20.810000000000002 80-84 24.205 28.175 27.04 20.580000000000002 85-89 24.11 28.375 26.484999999999996 21.029999999999998 90-94 24.68746874687469 27.15271527152715 27.21272127212721 20.94709470947095 95-99 25.009999999999998 27.83 26.515 20.645 100-104 24.01 28.63 26.735 20.625 105-109 25.44 27.095000000000002 26.625 20.84 110-114 23.755000000000003 28.205000000000002 26.755000000000003 21.285 115-119 24.945 29.035 26.810000000000002 19.21 120-124 25.922592259225922 27.892789278927893 26.137613761376137 20.047004700470048 125-129 25.48254825482548 29.027902790279025 25.48254825482548 20.00700070007001 130-134 26.82 28.29 25.485000000000003 19.405 135-139 26.795718287314923 27.81612645058023 25.820328131252502 19.56782713085234 140-144 27.63881940970485 28.634317158579293 25.297648824412207 18.429214607303653 145-149 27.51375687843922 28.209104552276138 25.942971485742873 18.33416708354177 150-151 28.251625812906454 27.826413206603302 24.662331165582792 19.259629814907452 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.5 4 0.5 5 0.5 6 1.5 7 1.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 1.5 14 2.0 15 0.5 16 0.0 17 0.0 18 1.5 19 2.0 20 1.0 21 0.5 22 0.0 23 1.0 24 2.5 25 2.5 26 2.5 27 3.0 28 3.5 29 6.5 30 7.0 31 9.0 32 13.5 33 18.5 34 36.0 35 53.5 36 63.0 37 80.5 38 100.5 39 146.0 40 193.0 41 247.5 42 261.5 43 257.0 44 286.5 45 266.0 46 255.0 47 260.0 48 245.0 49 210.5 50 176.5 51 163.5 52 139.5 53 95.0 54 70.5 55 61.5 56 43.0 57 36.5 58 31.5 59 25.5 60 22.5 61 13.0 62 6.5 63 7.0 64 6.0 65 4.0 66 2.5 67 2.0 68 2.5 69 2.0 70 2.0 71 1.5 72 1.5 73 2.0 74 2.0 75 1.5 76 1.0 77 1.5 78 1.5 79 1.0 80 0.5 81 0.0 82 1.0 83 1.0 84 1.0 85 1.5 86 0.5 87 0.5 88 1.0 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 1.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.01 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.01 125-129 0.01 130-134 0.0 135-139 0.04 140-144 0.05 145-149 0.05 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.875 #Duplication Level Percentage of deduplicated Percentage of total 1 63.29853862212944 37.9 2 21.210855949895617 25.4 3 8.434237995824635 15.15 4 3.549060542797495 8.5 5 1.7118997912317326 5.125 6 0.8350730688935281 3.0 7 0.5010438413361169 2.1 8 0.25052192066805845 1.2 9 0.0 0.0 >10 0.20876826722338201 1.625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 19 0.475 No Hit AAAACTATTATTTTCGTGAGATAGGGGGTGGAGCTGTGTGGTTTATTTAC 14 0.35000000000000003 No Hit GCAGTTCAATACTGTCTCGCATGACTGGAGAGATGCTTCCAGTGATGTCA 12 0.3 No Hit CAGCAAGTGCTTCTGTAGCTTTGACATCTTTGTCAAATGAAAAGCTCTTC 10 0.25 No Hit GCCAAACTACCAGCCTGAAATGCCATATAAAGGACGAGAGTTTTAAGATT 10 0.25 No Hit CCTGAGAATATGGTAGGCAAGGGTGGTAGTAGTCATGTTTACAAAGGGTG 8 0.2 No Hit GGCAAGTGGATTGATTACTTCTGATTTTCAAGACTTTGCTAATGCTGCTA 8 0.2 No Hit GAATCTTTTTTGCAAACTTTAGGCAACAATCTGATTTGCGAAGAGAATTT 8 0.2 No Hit GGAAAGACCACATTCGTTAAGAGGCATCTTACCGGAGAGTTCGAGAAGAA 8 0.2 No Hit GGCTGACTGACTGAGAGTGCGCACGCCCGCCCTTCCTTCGTTCCTGACCT 8 0.2 No Hit GACAGCCCAGATGGTAAGGCAAGATATTCTGTGACTTGTTACTATGCTAA 8 0.2 No Hit CTATTACTTTGGGTCCCCAAGGTGGACTTGCAAGAGGAATGTCCATTAGA 7 0.17500000000000002 No Hit TAGATACTAGTAGTAGTTGCAGACAGAGAGCTAGTGTTAGACAGGTTCTT 7 0.17500000000000002 No Hit GGTCGATTAACACCATCCAGAATTGGGATTGGTTGAGTATACATTCTGAG 7 0.17500000000000002 No Hit GCGAAGGAGCGAGCGGTGGACGGCCTGTCTCTGCCTCCGTCTCCATCTCC 7 0.17500000000000002 No Hit TGGAAACTGTACATTCATTTCACTGTTGATTTCCCAGACTCCCTGTCCCT 7 0.17500000000000002 No Hit GGAGGAGAGCATAACCATTTTAGTTAGTCACATATATTTCCAAGATGAAG 7 0.17500000000000002 No Hit GGCTTGAGCAGATTCATTCGCCAACTAACCCTTTAATTTATCCTATTTTT 7 0.17500000000000002 No Hit CAACTATTTAAGACCTGACCTTAAACGTGGCAATTTCACCGAAGCAGAAG 7 0.17500000000000002 No Hit TTTGAGACAGAGAGTTCGCCATTATGAACGGCAGAGATGGGCAACCATGG 7 0.17500000000000002 No Hit GGTGTGTCAAGTATTGAGTGTATTTATATAAATTATTTTTTGGACGGAAA 7 0.17500000000000002 No Hit AAAGGGAGCAACAAGCAAGGCATTCATTAGAACAGTCACCAATGTGGGTC 7 0.17500000000000002 No Hit TGTGCAGGCACTAAATAAGATGCGCACTGCCTAGGTAGGTAGAAGAAGGC 7 0.17500000000000002 No Hit GGAAGCTGGAAAGCGTTATTTCTTCACCAGGAATCACTCTACCATCCTTG 6 0.15 No Hit GCAGTAAAGCTTGATGGATCACATTACTTTTTCACTCTTTGGGTGCCTGG 6 0.15 No Hit CATGGTGGCCGCCAAGAAGACAAAGAAGACTCATGAGTCTATCAATAACA 6 0.15 No Hit AGGAACGAGATTGCTGGTGAAAAAAGTGAGCAGAAGGTGAAGATTGGTGA 6 0.15 No Hit CATAGATTTGTGTTGATATAGAAAACAATGGCACTACATGGAAAGATTGA 6 0.15 No Hit ACCACGAACAAGGATGCCACTATTCCATCTGATGAGAAAACAGGTTCGGC 6 0.15 No Hit GACGTTTAGAAATCCAAGTCTGTTTAAAAAACAAGTAAAACCCTACTTTC 6 0.15 No Hit CCTCTCTCTCTCTTCTTCCTCCTTGAATCTCTCCTCTTTGTGGAGGGGTC 6 0.15 No Hit AAGGCAATGCTGTATCACTTGATTCTAGTAGTAATGGTGATGGTAATGAT 6 0.15 No Hit CTTTATAGCCAACTCTGGGTGGTTGATTTTTCAGATAATGATCTGACTGG 6 0.15 No Hit TCAGCATGCTCTTTTGACTAGGAAAATGAACTCTATGAAGGACAGTAGCA 6 0.15 No Hit GCAGTCAAACCTGAATTGGGTCCAGCAATATCAGAAAGGGTATGGGAAGC 6 0.15 No Hit CGGCTGCCCAATCGAGCTAGTTGAGCCTGAAATCCTCCGATTCAAGGCCT 6 0.15 No Hit ATCGAATCAAACATCATTTACATCATTGCGTCCTTGCAGTGCAGAAACAA 6 0.15 No Hit TGGGTCTATGTCTGCTGAGAATCTTGTCAGGGAAATCATGAATTCTTTCA 6 0.15 No Hit GATTACTTTTTGCGGCAAATTGTCAAGGTATGCTCATTTTCTTATTTGCT 6 0.15 No Hit GACGAGGATGATGAGGACGAGGACGATGATGATGACACCCCCGGTGCTAA 6 0.15 No Hit GGTGAAGAGAGTGGTGCTGTTGAGACCAAGGATCGCGGGTTGTTTGATTT 6 0.15 No Hit CCTCAAAGAGCAGTGGAGTCCTGCTCTTACCATTTCTAAGGTACTGCTCT 6 0.15 No Hit GGTTCAAAGAACTTCCAGTTGATGGTCAAGTGGAGCTGATAAGTAAGCTC 6 0.15 No Hit GGAATTTCTCAGGTCGCAAGATTGAGCATTCGTTGAAGAAGATGTGCAAG 5 0.125 No Hit CCTCAAGGAAGGAATGGGCGGCGGCGGCAGCGGCGCCCATGATCCATTTG 5 0.125 No Hit AGAAGAGAGATGGGGGAGATGGAGGGAGAGAATGGAGGAGAGCTGAAAGA 5 0.125 No Hit GGGGAATCCAAATTTACAGCACTTGGGGCTGATTCACTTGATACGGTTGA 5 0.125 No Hit AGAAAACAATGGCACTACATGGAAAGATTGAGACAACATTAGAACTCAAG 5 0.125 No Hit GTGGGTACAACCAACAAGAGCACAGGGGCGACGCACAAGGTGAGCGCAAG 5 0.125 No Hit TGCTTCTGGTATGGCCGTCTCTCGCATCCTTCGCACCTCTCTCGGTCCCA 5 0.125 No Hit GATTTTTCTTTTAGTGCTGGTATATGTTTGGTTTTGCTACCGGAGTGGGC 5 0.125 No Hit TGAGATCTTTGTTGCTGTGAAGATCCTGAACAACTCAACAGGAAACGGAG 5 0.125 No Hit AAAGAGCTTAAAAAACACTGCTCCTTATCTCCCAAAACCCACTAAACTAA 5 0.125 No Hit CTCTTTCTGCCCCAGAGCCGCACCAACTCTTCGTCTCAGAGATGGCCAAG 5 0.125 No Hit GTTTGATAGGTATGTTGACACCTTTAACCAAGGTGGTGGAAAACCAGCAA 5 0.125 No Hit CAACAGCCACGAGTTCTTCATCAACACAAAAGCTCTCTCCTTCTCTTGGC 5 0.125 No Hit GGAATAAACCAGGATGCAATGATTGTGTGGGAAGATTTCATGTCTGAAGA 5 0.125 No Hit AAGAGACTCGCAAGAAGATTTGGCTGACAGATTCAAAGGGTTTGATTGTT 5 0.125 No Hit GGAAGATGTTGGCCATGGAAACAACGGAAGTCCATTTACCACGGGATTTG 5 0.125 No Hit AAACAACAACAAAGAGCTGTCGGAAGAAAGAAATTAGAAGATGCACAATC 5 0.125 No Hit GATATCTAGAAGTGGAGGGAGGGCTGCAAACTTGTGAGAAGGTGGTCTCT 5 0.125 No Hit CTTTGCTAATGCTGCTAAGAAGCTAGCTAATCATGCTATCAAGCTTGGCG 5 0.125 No Hit GCTAGATACTAGTAGTAGTTGCAGACAGAGAGCTAGTGTTAGACAGGTTC 5 0.125 No Hit GTTTTCATCTGGGCATGGAGCAATTGGGATGTATGCCAGCACAGATGGAC 5 0.125 No Hit CGTCCTTCAGAGCCCTCTATTATATGTCTGAAGCTTTGTCACGGCTGGGA 5 0.125 No Hit GCTCATTTTCCCTTCTCGATAAGGTGATTGAAGAGTATCAAAAGAATTTC 5 0.125 No Hit GCTCACCGAAGACCAGATCTCCGAGTTCAAGGAAGCTTTCAGCCTATTCG 5 0.125 No Hit GGTTGATCCCAGACTTTGTATTGTGAAACTTTGCATCAAAATCTGCATAG 5 0.125 No Hit GAAGAAGATGGTGGGTTTCAGAGATTTGTGATTCCAATTTCGTATCTATA 5 0.125 No Hit GATGGACAGAGGAAGAGCATAAGAAGTTCCTTGATGCTCTAAAGCTTTAC 5 0.125 No Hit GTAAAATCGTTGTCGAACTAAATGGAAGGTTGAACAAATGTGGGGTTATC 5 0.125 No Hit GTCTTTCTCAGAGTATCCTCCACTTGGTCGATTTGCTGTGAGGGACATGC 5 0.125 No Hit ATTTTCTCACACAAGACCCATTCAAGCTTGTAGCTGATGGCAGTCACCAA 5 0.125 No Hit AGTGGTGATTATAATTGTGACTCTGTGGACCCTTGGATGCTTGATGATCT 5 0.125 No Hit GGGGCTGGCAGGAGTTGTTGCCGTTCATGTTCCAGTGTGTTACCGCTCAA 5 0.125 No Hit CTGAGGAAGAGGGCTGATGCAGACAAGAACGACAAATCAGTGAAGGACTT 5 0.125 No Hit AGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTG 5 0.125 No Hit GTTGGGGTTTTGGTGATTGTGAATTTGGTGGGATTGTTGGTGCAAAGTGT 5 0.125 No Hit CTCGAGGGAAGCAGAAGATCGAAGCCCAGAGGAAAAACGCAGAAAGGAAT 5 0.125 No Hit CACACTTCAATTTCTCTCAAAAAGACCAAACAATGGAAGTCACCAAAACA 5 0.125 No Hit GTGAAGCCATCCCCACAGCCCGATAGTTGGAAACTTAAAGAGATATTTAG 5 0.125 No Hit AGGCTTTGGCAACCCAACTAGTGGAGCTGGCGGTGTTTTTGGTGGTACAT 5 0.125 No Hit CAAATGGCTGCATGTCAACTGGCCCGCATTTTAATCCTGTAGGCAAAGAG 5 0.125 No Hit GTTTTATTATCTCACAAGATCTGTCATGGCTACCCTTTCATCTTCTGCTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0125 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.0625 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.1375 0.0 0.0 0.0 0.0 80-81 0.1875 0.0 0.0 0.0 0.0 82-83 0.21250000000000002 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.30000000000000004 0.0 0.0 0.0 0.0 88-89 0.3375 0.0 0.0 0.0 0.0 90-91 0.375 0.0 0.0 0.0 0.0 92-93 0.5625 0.0 0.0 0.0 0.0 94-95 0.6375 0.0 0.0 0.0 0.0 96-97 0.75 0.0 0.0 0.0 0.0 98-99 1.0875 0.0 0.0 0.0 0.0 100-101 1.375 0.0 0.0 0.0 0.0 102-103 1.7125 0.0 0.0 0.0 0.0 104-105 1.95 0.0 0.0 0.0 0.0 106-107 2.325 0.0 0.0 0.0 0.0 108-109 2.9124999999999996 0.0 0.0 0.0 0.0 110-111 3.3 0.0 0.0 0.0 0.0 112-113 3.7875 0.0 0.0 0.0 0.0 114-115 4.3 0.0 0.0 0.0 0.0 116-117 5.025 0.0 0.0 0.0 0.0 118-119 5.6125 0.0 0.0 0.0 0.0 120-121 6.262499999999999 0.0 0.0 0.0 0.0 122-123 7.025 0.0 0.0 0.0 0.0 124-125 7.6 0.0 0.0 0.0 0.0 126-127 8.725000000000001 0.0 0.0 0.0 0.0 128-129 9.7 0.0 0.0 0.0 0.0 130-131 10.9 0.0 0.0 0.0 0.0 132-133 11.5625 0.0 0.0 0.0 0.0 134-135 12.649999999999999 0.0 0.0 0.0 0.0 136-137 13.5625 0.0 0.0 0.0 0.0 138-139 14.125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGGGAC 10 0.006830828 145.0 6 ATGTTTG 10 0.006830828 145.0 4 >>END_MODULE Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033925 spots for SRR26075401.sra Written 1033925 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra Read 1033908 spots for SRR26075401.sra Written 1033908 spots for SRR26075401.sra SRR ids: ['SRR26075401.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_5ttdxom4 SRR26075401.sra spots: 20678177 blocks: [[1, 1033908], [1033909, 2067816], [2067817, 3101724], [3101725, 4135632], [4135633, 5169540], [5169541, 6203448], [6203449, 7237356], [7237357, 8271264], [8271265, 9305172], [9305173, 10339080], [10339081, 11372988], [11372989, 12406896], [12406897, 13440804], [13440805, 14474712], [14474713, 15508620], [15508621, 16542528], [16542529, 17576436], [17576437, 18610344], [18610345, 19644252], [19644253, 20678177]] SRR26075401 file size 7631700 SRR26075401 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075401 SRR26075401_1.fastq SRR26075401_2.fastq Input file: SRR26075401_1.fastq Paired file: SRR26075401_2.fastq trimmed: SRR26075401-trimmed-pair1.fastq, SRR26075401-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:08:24 2025 >> started Wed Feb 12 01:08:55 2025 >> done (31.209s) 20678177 read pairs processed; of these: 133 ( 0.00%) short read pairs filtered out after trimming by size control 42886 ( 0.21%) empty read pairs filtered out after trimming by size control 20635158 (99.79%) read pairs available; of these: 3996525 (19.37%) trimmed read pairs available after processing 16638633 (80.63%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 10 0.00% 20 15 0.00% 21 11 0.00% 22 22 0.00% 23 8 0.00% 24 23 0.00% 25 17 0.00% 26 11 0.00% 27 17 0.00% 28 34 0.00% 29 36 0.00% 30 27 0.00% 31 26 0.00% 32 21 0.00% 33 32 0.00% 34 27 0.00% 35 30 0.00% 36 42 0.00% 37 33 0.00% 38 46 0.00% 39 54 0.00% 40 59 0.00% 41 72 0.00% 42 65 0.00% 43 52 0.00% 44 63 0.00% 45 67 0.00% 46 90 0.00% 47 84 0.00% 48 92 0.00% 49 106 0.00% 50 96 0.00% 51 144 0.00% 52 148 0.00% 53 194 0.00% 54 214 0.00% 55 249 0.00% 56 222 0.00% 57 236 0.00% 58 309 0.00% 59 325 0.00% 60 411 0.00% 61 391 0.00% 62 514 0.00% 63 590 0.00% 64 649 0.00% 65 728 0.00% 66 798 0.00% 67 845 0.00% 68 908 0.00% 69 1322 0.01% 70 1404 0.01% 71 1526 0.01% 72 1790 0.01% 73 2051 0.01% 74 2212 0.01% 75 2582 0.01% 76 3109 0.02% 77 3412 0.02% 78 3686 0.02% 79 4578 0.02% 80 4854 0.02% 81 5584 0.03% 82 6557 0.03% 83 7112 0.03% 84 8030 0.04% 85 9281 0.04% 86 10404 0.05% 87 10911 0.05% 88 11534 0.06% 89 12669 0.06% 90 14006 0.07% 91 15069 0.07% 92 16439 0.08% 93 17960 0.09% 94 20252 0.10% 95 21039 0.10% 96 23297 0.11% 97 25037 0.12% 98 26438 0.13% 99 27962 0.14% 100 28909 0.14% 101 30025 0.15% 102 32271 0.16% 103 34600 0.17% 104 36733 0.18% 105 38652 0.19% 106 41479 0.20% 107 43210 0.21% 108 44758 0.22% 109 46246 0.22% 110 47420 0.23% 111 49252 0.24% 112 50964 0.25% 113 52061 0.25% 114 54179 0.26% 115 56832 0.28% 116 59101 0.29% 117 61482 0.30% 118 63857 0.31% 119 64549 0.31% 120 65811 0.32% 121 68108 0.33% 122 68374 0.33% 123 70905 0.34% 124 72562 0.35% 125 73417 0.36% 126 76105 0.37% 127 78010 0.38% 128 79261 0.38% 129 82030 0.40% 130 83080 0.40% 131 83465 0.40% 132 84962 0.41% 133 86643 0.42% 134 86379 0.42% 135 88019 0.43% 136 90810 0.44% 137 91641 0.44% 138 92853 0.45% 139 94689 0.46% 140 96938 0.47% 141 95775 0.46% 142 98739 0.48% 143 98577 0.48% 144 101011 0.49% 145 101636 0.49% 146 101565 0.49% 147 102377 0.50% 148 103729 0.50% 149 105127 0.51% 150 105973 0.51% 151 16638633 80.63% 20635158 reads passed initial QC criterion=sequence-density sequence-density=0.31 sequence-density-rank=1 fanout-score=2.82 fanout-score-rank=31 prefix-density=0.42 prefix-fanout=2.1 sequence=CACTTGCAGCCATTCTCAGCACCAGAG criterion=fanout-score sequence-density=0.02 sequence-density-rank=40 fanout-score=175.03 fanout-score-rank=1 prefix-density=0.33 prefix-fanout=11.4 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=2.83 fanout-score-rank=36 prefix-density=0.35 prefix-fanout=2.8 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=226.55 fanout-score-rank=1 prefix-density=0.19 prefix-fanout=9.4 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGGCAAGCTTAGAAAGATCAGCACCAGACCACTTAACATCAAAGATATTGACGTCGGAGAGGGGAGCAGTCCTGTTAATAAGTTTCAGAGGTCCATGACTATGCCAGGAACTCCAGGGACACCGACGACACCAGTGACCCCTACAACCCCAGTGTCGGCGCGTAGCAATGTTTGGAGGAGCGTGTTCCACCCTGGTAGCAACCTTGCTACTAAGAATATTGGTGCTCATGTTTTTGACAAGCCACAGCCTAACACACCCACTGTCTATGACTGGATGTACAGTGGAGAGACGAAGAGCGAGCATCGTTGATGAGGTTGCCTTCAACCAAGGTTGCCCATGTAAATACGTACTGTGTTTTGTTTTTCAGTACTCATCTGCAATATGTCTCTTGTTGTTATGGTTCTACGGTTCTACCGTGCCTGGAA SRR26075401 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:09:36 Started mapping on | Feb 12 01:09:36 Finished on | Feb 12 01:12:32 Mapping speed, Million of reads per hour | 422.08 Number of input reads | 20635158 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 18716620 Uniquely mapped reads % | 90.70% Average mapped length | 290.92 Number of splices: Total | 17525267 Number of splices: Annotated (sjdb) | 17104677 Number of splices: GT/AG | 17191884 Number of splices: GC/AG | 259807 Number of splices: AT/AC | 16994 Number of splices: Non-canonical | 56582 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.96 Insertion rate per base | 0.02% Insertion average length | 2.52 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 698146 % of reads mapped to multiple loci | 3.38% Number of reads mapped to too many loci | 59048 % of reads mapped to too many loci | 0.29% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.27% % of reads unmapped: other | 0.36% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1220392 1220392 1220392 N_multimapping 698146 698146 698146 N_noFeature 518578 18517475 624592 N_ambiguous 201002 913 107321 UnstrandedReadsAssigned:17997040 PositiveStrandReadsAssigned:198232 NegativeStrandReadsAssigned:17984707 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075401 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075401-trimmed-pair1.fastq SRR26075401-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,635,158 reads, 18,314,525 reads pseudoaligned [quant] estimated average fragment length: 203.998 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,228 rounds 52401 SRR26075401.ke.tsv 34699 SRR26075401.se.tsv 87100 total ==> SRR26075401.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1815 1770 49.1462 Potri.005G024800.1.v4.1 1035 832.002 1108 67.1134 Potri.004G059700.1.v4.1 961 758.009 0 0 Potri.007G009000.2.v4.1 1416 1213 0 0 Potri.003G141000.2.v4.1 2943 2740 801.365 14.7392 Potri.016G087400.1.v4.1 270 94.1438 1755.62 939.792 Potri.015G069301.1.v4.1 564 362.282 0 0 Potri.010G195200.1.v4.1 1773 1570 592 19.0027 Potri.012G127500.1.v4.1 977 774.002 11782 767.133 ==> SRR26075401.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 123 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 269 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 1 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 659 SRR26075401 completed mapping pipeline successfully