Starting /dee2/code/volunteer_pipeline.sh SRR26075402 current disk space = 3051074121728 free memory = 1491001384 SRR26075402 SRAfilesize f559f01e3ccdce9c6acfbbf537f018fd SRR26075402.sra SRR26075402.sra file validated SRR26075402 is paired end SRR26075402 is conventional basespace SRR26075402 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075402_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.614 37.0 37.0 37.0 37.0 37.0 2 36.5575 37.0 37.0 37.0 37.0 37.0 3 36.616 37.0 37.0 37.0 37.0 37.0 4 36.6245 37.0 37.0 37.0 37.0 37.0 5 36.684 37.0 37.0 37.0 37.0 37.0 6 36.6545 37.0 37.0 37.0 37.0 37.0 7 36.6115 37.0 37.0 37.0 37.0 37.0 8 36.6415 37.0 37.0 37.0 37.0 37.0 9 36.6145 37.0 37.0 37.0 37.0 37.0 10-14 36.6472 37.0 37.0 37.0 37.0 37.0 15-19 36.613099999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.488099999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.42100000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.435700000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.3834 37.0 37.0 37.0 37.0 37.0 40-44 36.22330000000001 37.0 37.0 37.0 37.0 37.0 45-49 35.08579999999999 37.0 37.0 37.0 29.8 37.0 50-54 35.0178 37.0 37.0 37.0 29.8 37.0 55-59 34.3241 37.0 37.0 37.0 25.0 37.0 60-64 34.4841 37.0 37.0 37.0 27.4 37.0 65-69 34.3482 37.0 37.0 37.0 24.6 37.0 70-74 35.219699999999996 37.0 37.0 37.0 29.8 37.0 75-79 35.973499999999994 37.0 37.0 37.0 37.0 37.0 80-84 36.0119 37.0 37.0 37.0 37.0 37.0 85-89 35.905699999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.851600000000005 37.0 37.0 37.0 37.0 37.0 95-99 35.886199999999995 37.0 37.0 37.0 37.0 37.0 100-104 35.8022 37.0 37.0 37.0 37.0 37.0 105-109 35.7481 37.0 37.0 37.0 37.0 37.0 110-114 35.5682 37.0 37.0 37.0 37.0 37.0 115-119 35.4701 37.0 37.0 37.0 37.0 37.0 120-124 35.4779 37.0 37.0 37.0 37.0 37.0 125-129 35.49679999999999 37.0 37.0 37.0 37.0 37.0 130-134 35.2602 37.0 37.0 37.0 34.6 37.0 135-139 35.1462 37.0 37.0 37.0 29.8 37.0 140-144 34.852999999999994 37.0 37.0 37.0 25.0 37.0 145-149 34.6682 37.0 37.0 37.0 25.0 37.0 150-151 34.43475 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 2.0 22 2.0 23 5.0 24 9.0 25 7.0 26 14.0 27 14.0 28 14.0 29 26.0 30 28.0 31 72.0 32 139.0 33 349.0 34 158.0 35 444.0 36 2568.0 37 148.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.344172086043024 12.581290645322662 12.481240620310155 36.59329664832416 2 18.4 21.349999999999998 31.025000000000002 29.225 3 17.349999999999998 17.075000000000003 33.525 32.05 4 18.625 24.224999999999998 21.15 36.0 5 30.099999999999998 27.55 22.85 19.5 6 28.849999999999998 31.7 21.525 17.925 7 13.25 33.175 37.175000000000004 16.400000000000002 8 17.5 32.125 26.724999999999998 23.65 9 23.425 23.1 31.974999999999998 21.5 10-14 20.0 30.620000000000005 25.330000000000002 24.05 15-19 19.945 27.435 27.47 25.15 20-24 21.435000000000002 28.794999999999998 27.35 22.42 25-29 19.91 28.13 26.105 25.855 30-34 20.585 27.315 26.77 25.330000000000002 35-39 18.18 29.054999999999996 28.305000000000003 24.46 40-44 19.42 27.18 26.424999999999997 26.974999999999998 45-49 21.955 26.105 27.99 23.95 50-54 22.415 25.335 27.96 24.29 55-59 22.865 25.865 27.675 23.595 60-64 24.02 25.31 27.93 22.74 65-69 22.53 27.435 28.04 21.995 70-74 27.200000000000003 26.08 24.425 22.295 75-79 27.215 24.955 24.740000000000002 23.09 80-84 27.465 24.990000000000002 25.53 22.015 85-89 27.765 24.965 25.064999999999998 22.205 90-94 27.21 26.07 24.275 22.445 95-99 27.810000000000002 24.25 25.929999999999996 22.009999999999998 100-104 27.700000000000003 25.6 24.52 22.18 105-109 27.639999999999997 25.995 23.755000000000003 22.61 110-114 28.155 25.405 24.12 22.32 115-119 27.495000000000005 25.535000000000004 24.8 22.17 120-124 28.449999999999996 25.83 24.15 21.57 125-129 29.43 24.895 23.865 21.81 130-134 28.595 25.014999999999997 24.205 22.185 135-139 28.37 26.015 23.1 22.515 140-144 28.485 24.705 24.485 22.325 145-149 29.310000000000002 24.59 23.73 22.37 150-151 29.1125 25.424999999999997 22.237499999999997 23.225 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 2.5 22 4.0 23 3.0 24 2.5 25 2.5 26 6.5 27 11.0 28 10.5 29 10.0 30 11.0 31 14.0 32 20.0 33 35.5 34 39.5 35 42.0 36 70.5 37 98.5 38 115.5 39 133.5 40 158.5 41 165.0 42 205.0 43 239.0 44 247.5 45 228.0 46 206.0 47 227.5 48 212.0 49 186.0 50 162.0 51 156.5 52 139.0 53 99.0 54 89.0 55 70.0 56 49.5 57 38.0 58 29.5 59 29.0 60 28.5 61 17.5 62 9.0 63 12.5 64 11.5 65 13.5 66 20.5 67 32.0 68 40.0 69 58.5 70 64.5 71 47.5 72 31.0 73 18.0 74 11.5 75 5.5 76 3.5 77 3.5 78 1.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.199999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 65.07867132867133 37.225 2 19.405594405594407 22.2 3 8.216783216783217 14.099999999999998 4 3.7587412587412583 8.6 5 1.617132867132867 4.625 6 0.6993006993006993 2.4 7 0.34965034965034963 1.4000000000000001 8 0.26223776223776224 1.2 9 0.13111888111888112 0.675 >10 0.4370629370629371 5.375 >50 0.043706293706293704 2.1999999999999997 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCAACCATCTCGTTT 88 2.1999999999999997 TruSeq Adapter, Index 9 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCAACCATCTCGTAT 48 1.2 TruSeq Adapter, Index 9 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCAACCATCGCGTTT 42 1.05 TruSeq Adapter, Index 9 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCAACCATCTCGGTT 26 0.65 TruSeq Adapter, Index 9 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCAACCATCGCGGTT 23 0.575 TruSeq Adapter, Index 9 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCAACCATCTCGTGT 18 0.44999999999999996 TruSeq Adapter, Index 9 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCAACCATCTCGGGT 14 0.35000000000000003 TruSeq Adapter, Index 9 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCAACCATCGCGTAT 12 0.3 TruSeq Adapter, Index 9 (97% over 38bp) CTCAACTGAAAAGTGCTTGACAGCCAAGTGCTCCTCCCAGTCATTGGTAA 11 0.27499999999999997 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCAACCATCGCGGGT 11 0.27499999999999997 TruSeq Adapter, Index 9 (97% over 38bp) CTCGGAGATTTCTTCTCCTCTAGGAAGTTCATCATCATCTCGAAAAGTGT 10 0.25 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCAACCATCTCGGAT 9 0.22499999999999998 TruSeq Adapter, Index 9 (97% over 38bp) GGAACAAGCACTACATCCACAAATCCCAAGCTTTCACCACCAAAGTAAAG 9 0.22499999999999998 No Hit GTTACATCATACACCAGAAGAGCTCCAAGCGCGCCCCTATAGTAGGCACT 9 0.22499999999999998 No Hit GGGCTGTACAACTGTCCCTGAGAATCACTCGAGAGGGTGAGGCAAACAGG 8 0.2 No Hit CCCAGAGCTGCAAATCCAAGAAGATTTGCAGAAAACAAGCCATGAATATA 8 0.2 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCAACCATCGCGTGT 8 0.2 TruSeq Adapter, Index 9 (97% over 38bp) AAGAGAAGTCAGTGGAAAACCTTCACGTTCTTTCTCCATCTTTACCTTCT 8 0.2 No Hit CTTCGGATTCACCCCTTGAAGAGTACCTAGATCCACCGTCACCATAACCA 8 0.2 No Hit AGTGCAAGGATCGCAGGTACAGTTGGCTCCACACTTGCAGCCATTCTCGG 8 0.2 No Hit GATGGAGCCGGTGCTTGTGCAACAGATGGTTGGTGTACAATATTTCCAGT 7 0.17500000000000002 No Hit TATTTGTGCAACAATGTCCTTGTTGCTAAATCGGACAACAAATCGATACT 7 0.17500000000000002 No Hit GGGTTTTGAGTAAACATCACTTCTAGTTATCGACAACTTCAATGAGGGTG 7 0.17500000000000002 No Hit GACCTATTTATGGGGATATTGAGCACATCACAAAAATCCATCAACTTCTC 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCAACCATCGCGGAT 7 0.17500000000000002 TruSeq Adapter, Index 9 (97% over 38bp) GTTAGATCTTGATGCTGAACGATATTGCTAAAATTTGACTCATCTAATAA 7 0.17500000000000002 No Hit GGCCTCTCTTCTCATCTCTAGCTGTCAACACCACAACAATCCCATGAGAA 7 0.17500000000000002 No Hit GTGCAAGGCCTGCAGAGCGCCTAGGTCCATGACTGAATAAAGCATTGATT 7 0.17500000000000002 No Hit CTTTGAAAGTGTCTCCTTGCACATATTCCCTTGTACCATAAGTGTCAAAA 6 0.15 No Hit GGTCATTGGAGATGGTTTTGGACCTTTCAGCTATGGAGAGAGGGTTGATA 6 0.15 No Hit GCAGTGTGTTACAAAGATTACAAAAGATGCCGCCATTAACAACTTGATCG 6 0.15 No Hit ACCCACTCCCTCGGATAGGAACTCCCACCACAGATCAGTATCTTAGGTCT 6 0.15 No Hit GTCCAAAACTGCAGGAGAAATTATCCAGGAAAAGTCACAATCTCTATTGT 6 0.15 No Hit GCCTCCAATGCCTTCTTGGCTTTGTAGTATGGGACATTTTTCACAACAAT 6 0.15 No Hit ACTAGTGACAAAAGATAACCTGCGGAAGTTTCCAAGCAATTATGCTCAAC 6 0.15 No Hit ATCCTGAATACCCCATCTGGTGGTTGTTTTCATGCATGGTTGGTGAGCTG 6 0.15 No Hit AAGCAAACTGTTAAATTCTGGAGGAGGGTGTGAGGAACCCCTCGGCCCTC 6 0.15 No Hit GCCTTCCACCAGTGCCCCGAAATGCAGGCTGGTAATCTGCTGGTGCTCCT 6 0.15 No Hit GCAATTGCATGGATCGCAGGTGCAGTTTGATCCGCACTTGCAGCCATTCT 6 0.15 No Hit AAATACCAAAGACAATGAGAGAGGACCACTTTTCTTCGAATTAGTGGGAA 6 0.15 No Hit TGGTTTTCATCATGATAATTCTGGTTTGGTTCTTGCATGAAGAAAGCAGG 6 0.15 No Hit TGCATAGTGGATAAAAATCCTTGTTAGCAACATAAAAGAAATCGTTTTGC 6 0.15 No Hit GCCACCTGATGATGCACCTACAGCATGAAAATTTGATGATGGACCCTCAC 6 0.15 No Hit GCGACAAGAAGAAGAATCAAAACCAGGAGGCAGATTCCCACCAGGGATGG 6 0.15 No Hit CTGTATTACATGGTTTCCATTTTGATCATGTACGCATCTCATAACATGCC 5 0.125 No Hit GCAAGCCCTTTTTATTGGTGGATCTAATCAAATCCAAACTCTCCGAGAAT 5 0.125 No Hit GCTTCTTGTACTTGTACAGTGTAGCAACATCCCCAGCTAAGAGTCCTCCT 5 0.125 No Hit TTGCTCTTCTTGACTTTCTTAAGCTTCCTCAAGAAGTACCAAAACTTGGA 5 0.125 No Hit GTGCTTTCTGAGATACCTAAGTCAGGGTACATGCCACATTTGCAGCCACT 5 0.125 No Hit CCCGGCGCAAATTAGCATCAATTGTGCCCTCACTATGCCCACAGCTCCTC 5 0.125 No Hit GTGACCAACAATATCAACTTTTATTTCAGTAAGAATTATTACATAGATGA 5 0.125 No Hit CTCGGAAATAGAAAAACCAGCAAAACAACCAACCATATCACATAAACAGG 5 0.125 No Hit CTCTGGAAGCACTGTGAGGGTTGCTGCCGTTAGGATCATCTTCTCCCCCA 5 0.125 No Hit ACTTCTTCATTCATCCTCCTGGCTGCGCAACCTGGCAAGCTTGTTCGTAA 5 0.125 No Hit GCTGTAGTGGAAGGAAACGGCTGTGATGGAGAGTGAGCGGCAGCGGCTGG 5 0.125 No Hit TCACCATTGTTGCGGATGAGAAAGTCCCTCTCTTCTGAAGAAAGAAGCTT 5 0.125 No Hit ATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGC 5 0.125 No Hit GCCTTAACTCCATGCCAAATTCATTACATTCCTTTTCTCCATGAAAACAT 5 0.125 No Hit CCTCAATTTCCGACAAACAGCAGGCATTAAGGGTGCTTTGGCTGTGCTCA 5 0.125 No Hit CTGGTCAATATTGGAATTTGAATTTTCTCGAGGACAGAATCTTGTTGAAT 5 0.125 No Hit TAATATAAGGCAAGAATCATCTCCTACACTACAAAACTCCTGCGCACTGG 5 0.125 No Hit GCCATCCTCGAGCTGCTTGCCGGCAAAGATCAACCTCTGCTGGTCTGGGG 5 0.125 No Hit CATACACTCGTCAGCATTCTCAACCAACTGATGAACTGAGAGAGTGGCGT 5 0.125 No Hit GTGACACTATTTCTTGCTGGGAGAATAGATTATGGCAGTGTAGTTGAACC 5 0.125 No Hit TTGGTGACGGAATATTGGATCAACTGTTAATGGTTTCTTGCTGTAGTCTA 5 0.125 No Hit TTCGAACATCTGTTTCAACCAGGAACTTGATGCTTCCATAGCCATGCCTT 5 0.125 No Hit CTCAGTTTGTGTCATAGGTTTAGTTGAAGGTGTAACACCTGCCTTCTCCA 5 0.125 No Hit CCGTCATATTCTTCTCCTGCCACTCAAAAAACACTGCATCTCTAATAAGC 5 0.125 No Hit CCTCTAATCCCTTTTCTTATCCTTCAATGGCCCCCTAGGAATCTTACTTA 5 0.125 No Hit CTGTGATCAAATAACAAAGAGCGTGACGCGACCAAACCCATAGCCACCAC 5 0.125 No Hit ACCGAATCCAAACCCTTCACTGGCTTCATGAATGGCAAGTAGCAACCTCT 5 0.125 No Hit TCACCCTCTATTAGCCTTGAAACCAGTTTGTCCACAAAATCGATAAAATC 5 0.125 No Hit TGGTAGCCACTGACTTTTTTTCAATCACATCACCATGTTCAAAATGAGGT 5 0.125 No Hit TCAATCTTGCACAAACCAGCCCACTCTCCTAGGGTCTTTGCGCTTGGAAC 5 0.125 No Hit CAGGCGTTCTGATCTGCAAGGAGCTCCAGTCTTGATCTGTCCAGTAGACA 5 0.125 No Hit TGCATTCTGAATATTTTTGATTCTTCAAAAGCTCTATCATGTTCAAGAAT 5 0.125 No Hit GCAGAAACAAGCTCAGAGAGGGTCATGGGTTTGCCATGTTTTTGGATCAC 5 0.125 No Hit GTTCGATCCTTTCCTTGGAATTATGGCACAATTTATCTCTTCCCCATATT 5 0.125 No Hit CATGTGTAGATGAAACTCGAACTGAAGTACCTGGGATATCTGCACTTTTA 5 0.125 No Hit CTGCAACTGGCAAAGAGTTAAAGACTTATGAACCAAGTAGCTTCTTCCAT 5 0.125 No Hit GGAAAATACAAAGGTATAACCTGAGAGTATACTAAACAACCGGAACCATA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.037500000000000006 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.0625 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.125 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.2625 0.0 0.0 0.0 0.0 72-73 0.325 0.0 0.0 0.0 0.0 74-75 0.325 0.0 0.0 0.0 0.0 76-77 0.35 0.0 0.0 0.0 0.0 78-79 0.44999999999999996 0.0 0.0 0.0 0.0 80-81 0.6375 0.0 0.0 0.0 0.0 82-83 0.825 0.0 0.0 0.0 0.0 84-85 0.85 0.0 0.0 0.0 0.0 86-87 0.9874999999999999 0.0 0.0 0.0 0.0 88-89 1.1625 0.0 0.0 0.0 0.0 90-91 1.275 0.0 0.0 0.0 0.0 92-93 1.5375 0.0 0.0 0.0 0.0 94-95 2.075 0.0 0.0 0.0 0.0 96-97 2.4625 0.0 0.0 0.0 0.0 98-99 3.1500000000000004 0.0 0.0 0.0 0.0 100-101 3.5125 0.0 0.0 0.0 0.0 102-103 4.075 0.0 0.0 0.0 0.0 104-105 4.7375 0.0 0.0 0.0 0.0 106-107 5.1125 0.0 0.0 0.0 0.0 108-109 5.475 0.0 0.0 0.0 0.0 110-111 5.9375 0.0 0.0 0.0 0.0 112-113 6.574999999999999 0.0 0.0 0.0 0.0 114-115 7.4125 0.0 0.0 0.0 0.0 116-117 8.1125 0.0 0.0 0.0 0.0 118-119 8.7875 0.0 0.0 0.0 0.0 120-121 9.475 0.0 0.0 0.0 0.0 122-123 10.3125 0.0 0.0 0.0 0.0 124-125 11.0 0.0 0.0 0.0 0.0 126-127 11.712499999999999 0.0 0.0 0.0 0.0 128-129 12.825 0.0 0.0 0.0 0.0 130-131 13.662500000000001 0.0 0.0 0.0 0.0 132-133 14.375 0.0 0.0 0.0 0.0 134-135 15.3125 0.0 0.0 0.0 0.0 136-137 16.625 0.0 0.0 0.0 0.0 138-139 17.6 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGAAT 10 0.006830828 145.0 3 TTTTTTG 10 0.006830828 145.0 6 GAATGTA 10 0.006830828 145.0 6 AGAATGT 10 0.006830828 145.0 5 GTTTAGA 10 0.006830828 145.0 1 TGTAGAG 10 0.006830828 145.0 9 AATGTAG 10 0.006830828 145.0 7 GATCGGA 130 7.4578566E-11 50.192307 1 GAAGAGC 130 7.4578566E-11 50.192307 6 GAGCACA 130 7.4578566E-11 50.192307 9 CGGAAGA 130 7.4578566E-11 50.192307 4 AGAGCAC 130 7.4578566E-11 50.192307 8 AAGAGCA 135 1.09139364E-10 48.333332 7 TCGGAAG 135 1.09139364E-10 48.333332 3 ATCGGAA 135 1.09139364E-10 48.333332 2 GGAAGAG 135 1.09139364E-10 48.333332 5 AGGGGGG 30 4.189703E-5 29.000002 65-69 TGCCGTC 25 4.977651E-4 29.0 50-54 CCGTCTT 20 0.00593511 29.0 50-54 AAGGGGG 20 0.00593511 29.0 65-69 >>END_MODULE SRR26075402 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075402_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.343 37.0 37.0 37.0 37.0 37.0 2 36.417 37.0 37.0 37.0 37.0 37.0 3 36.3965 37.0 37.0 37.0 37.0 37.0 4 36.3125 37.0 37.0 37.0 37.0 37.0 5 36.1975 37.0 37.0 37.0 37.0 37.0 6 36.1635 37.0 37.0 37.0 37.0 37.0 7 36.1105 37.0 37.0 37.0 37.0 37.0 8 36.0905 37.0 37.0 37.0 37.0 37.0 9 36.0165 37.0 37.0 37.0 37.0 37.0 10-14 35.8591 37.0 37.0 37.0 37.0 37.0 15-19 35.665299999999995 37.0 37.0 37.0 37.0 37.0 20-24 35.468 37.0 37.0 37.0 37.0 37.0 25-29 34.8394 37.0 37.0 37.0 25.0 37.0 30-34 34.6318 37.0 37.0 37.0 25.0 37.0 35-39 34.116200000000006 37.0 37.0 37.0 25.0 37.0 40-44 33.978899999999996 37.0 37.0 37.0 25.0 37.0 45-49 33.8378 37.0 37.0 37.0 16.6 37.0 50-54 33.5096 37.0 37.0 37.0 11.0 37.0 55-59 33.7302 37.0 37.0 37.0 16.6 37.0 60-64 34.013099999999994 37.0 37.0 37.0 25.0 37.0 65-69 33.7232 37.0 37.0 37.0 13.8 37.0 70-74 33.551700000000004 37.0 37.0 37.0 11.0 37.0 75-79 33.4636 37.0 37.0 37.0 11.0 37.0 80-84 33.662 37.0 37.0 37.0 16.6 37.0 85-89 33.9591 37.0 37.0 37.0 25.0 37.0 90-94 34.299400000000006 37.0 37.0 37.0 25.0 37.0 95-99 34.679199999999994 37.0 37.0 37.0 25.0 37.0 100-104 34.8627 37.0 37.0 37.0 25.0 37.0 105-109 34.9751 37.0 37.0 37.0 27.4 37.0 110-114 35.0754 37.0 37.0 37.0 27.4 37.0 115-119 34.874 37.0 37.0 37.0 25.0 37.0 120-124 34.8662 37.0 37.0 37.0 25.0 37.0 125-129 34.9154 37.0 37.0 37.0 25.0 37.0 130-134 34.8069 37.0 37.0 37.0 25.0 37.0 135-139 34.6858 37.0 37.0 37.0 25.0 37.0 140-144 34.699 37.0 37.0 37.0 25.0 37.0 145-149 34.6434 37.0 37.0 37.0 25.0 37.0 150-151 34.508 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 4.0 13 6.0 14 9.0 15 26.0 16 14.0 17 10.0 18 12.0 19 9.0 20 15.0 21 18.0 22 20.0 23 29.0 24 40.0 25 65.0 26 84.0 27 78.0 28 44.0 29 26.0 30 24.0 31 35.0 32 53.0 33 66.0 34 177.0 35 614.0 36 2304.0 37 218.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.824999999999996 19.7 15.975 25.5 2 38.800000000000004 19.075 24.349999999999998 17.775 3 30.5 25.374999999999996 24.525 19.6 4 34.050000000000004 29.275000000000002 20.4 16.275000000000002 5 33.425 30.45 20.275000000000002 15.85 6 29.5 33.925 20.825 15.75 7 28.549999999999997 19.25 34.325 17.875 8 31.4 22.575 22.775000000000002 23.25 9 30.55 21.15 26.950000000000003 21.349999999999998 10-14 32.21 26.229999999999997 22.15 19.41 15-19 31.929999999999996 24.465 24.505 19.1 20-24 31.59 25.36 23.93 19.12 25-29 31.830000000000002 25.485000000000003 24.255 18.43 30-34 30.415 25.645 24.64 19.3 35-39 30.25 25.865 24.19 19.695 40-44 31.430000000000003 25.735000000000003 23.830000000000002 19.005 45-49 30.64 25.345000000000002 24.39 19.625 50-54 22.8 26.490000000000002 30.919999999999998 19.79 55-59 29.555 24.77 26.135 19.54 60-64 31.045 25.855 24.395 18.705 65-69 30.89 25.985000000000003 23.95 19.175 70-74 26.055 29.98 23.93 20.035 75-79 26.295 29.565 24.91 19.23 80-84 28.99 26.540000000000003 24.575 19.895 85-89 31.1 25.83 24.43 18.64 90-94 31.135 26.58 23.595 18.69 95-99 31.724999999999998 26.669999999999998 23.669999999999998 17.935000000000002 100-104 32.714999999999996 25.979999999999997 23.369999999999997 17.935000000000002 105-109 32.335 25.56 24.015 18.09 110-114 32.015 25.255 25.055 17.675 115-119 31.955 25.995 24.305 17.745 120-124 32.43 26.58 23.89 17.1 125-129 32.95 27.055 22.869999999999997 17.125 130-134 32.055 26.515 24.05 17.380000000000003 135-139 32.885 26.415 23.78 16.919999999999998 140-144 33.33 26.715 22.64 17.315 145-149 33.57 26.040000000000003 23.595 16.794999999999998 150-151 34.4875 25.55 22.775000000000002 17.1875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 1.5 9 1.5 10 0.0 11 0.5 12 1.5 13 2.0 14 1.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.5 20 1.0 21 0.5 22 0.0 23 0.5 24 1.0 25 1.5 26 2.5 27 4.5 28 4.0 29 2.5 30 4.5 31 12.5 32 14.5 33 13.0 34 33.5 35 51.0 36 54.0 37 68.5 38 89.5 39 115.0 40 149.5 41 188.0 42 238.0 43 262.5 44 252.5 45 241.5 46 224.0 47 207.0 48 212.5 49 196.0 50 157.5 51 142.0 52 122.0 53 106.0 54 93.0 55 75.0 56 55.0 57 44.5 58 36.0 59 24.0 60 24.5 61 16.5 62 7.5 63 9.0 64 9.0 65 9.0 66 9.0 67 10.5 68 9.0 69 4.0 70 3.5 71 3.0 72 2.5 73 2.0 74 2.5 75 2.0 76 2.0 77 4.5 78 7.0 79 11.0 80 11.0 81 7.0 82 11.0 83 17.5 84 21.5 85 24.0 86 23.5 87 23.5 88 25.5 89 32.0 90 27.0 91 19.5 92 17.5 93 13.5 94 12.5 95 7.5 96 5.5 97 6.0 98 4.5 99 4.0 100 14.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.375 #Duplication Level Percentage of deduplicated Percentage of total 1 67.41052631578948 40.025 2 17.852631578947367 21.2 3 8.16842105263158 14.549999999999999 4 3.0736842105263156 7.3 5 1.7263157894736842 5.125 6 0.8421052631578947 3.0 7 0.2947368421052632 1.225 8 0.3368421052631579 1.6 9 0.12631578947368421 0.675 >10 0.08421052631578947 0.525 >50 0.042105263157894736 1.7500000000000002 >100 0.042105263157894736 3.025 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 121 3.025 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 70 1.7500000000000002 No Hit GCAGAAACCCATCTGGATGAGGAAGCCTGATGAGATTACCAAGGAGGAGT 11 0.27499999999999997 No Hit ATAGCTAACTCTTTGTTATACAGAGCTGAAATGGCAGAGCCAGAGTCTTC 10 0.25 No Hit AGACAGAGAAAGAGGAGGAGATGGCGAGGAGACCCGACGAGGAGTACGAT 9 0.22499999999999998 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGT 9 0.22499999999999998 No Hit AGGAGAAGAGCAAGAAGAGGTAAAGAGGGAATTTATAGAAGGCTTGAAGA 9 0.22499999999999998 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTT 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGT 8 0.2 No Hit CTTGTAAATGAGAGCGATCCGCTGGCTGCGTTGATTCAAGAAATGATCAT 8 0.2 No Hit AAGTGCGGCAGCGGCTGTGGAGGATGCAAGATGTACCCTGACATGAGCTC 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGT 8 0.2 No Hit AGCGGATATGGTAGTGGTGGCGGCGGCGGTGGTTATGGCGGTGGCCGTGA 8 0.2 No Hit TGACTACATGGATATTGATGAGGAACACAATAACAGTGATTCTGATATAG 8 0.2 No Hit CGGATGGATTTAGCCAGATTGCTCCGTATGATCAGTTACAAACAATGGAA 8 0.2 No Hit GGAACACCTCTGCAATCTGCGAACTCGCCTTTTGTTGTCCCATCTCCTTC 7 0.17500000000000002 No Hit GCGGTGTCATCAATTCCATCATGTAAACTCATTGAAGAAACATGCGAGAG 7 0.17500000000000002 No Hit AGAAACATCCACTGCAACAAAGAGATATGCAGTCGTGACAGGAGCAAATA 7 0.17500000000000002 No Hit CCCCCTCCTTCCCCTCGTCCCCCTCTAACAGATCAAGCTCCAACAATGGC 7 0.17500000000000002 No Hit CCTGGATTGAAAGCTGGTGTCAGGTTCCGCAATGATGTTCCCACTGGTGG 7 0.17500000000000002 No Hit CCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGA 7 0.17500000000000002 No Hit TGACAATCTGCAGATGCTTCACTTGATTCTGTTTGGAAAGAAAGGAAAGG 7 0.17500000000000002 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT 6 0.15 No Hit TGGTGCCCGGACTGGACAATTCGATCATCTTCTCCCTTCATCAATTGGTT 6 0.15 No Hit TTTGACTGAAAATGGAAATACCAAGGATGATCTGAGGCTTCCCACTGATG 6 0.15 No Hit GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA 6 0.15 No Hit GGCCATTTGGTGGCCCACCTGGTGACCGCCCACGAGGCCCACCTCGCTTT 6 0.15 No Hit GCTCATTGGATCATCGTTAAAACCTACCTGTCAGCTACTTGAATCTGATA 6 0.15 No Hit GAAAAATAAAGCACTCAATTAAATTGTTGTTCAGTGTCATTCAGTTTAAA 6 0.15 No Hit CATAGTTCCATGGCTAGTCCAGCACAACTCGTGCCCTGTTTGTCGGCAGG 6 0.15 No Hit TGGGTATTATACACCGGGAGGGAAAAGGGTCTCGGCTTCTTCGATTTTTT 6 0.15 No Hit GAGAAAGAAAGTTTCAACTTCTTTGCCAAACCATTCGATGAAAAGCAAAA 6 0.15 No Hit CAACTACTACCGTGCTATGGACCTGAACTGGGAGCTTTCTGCACCTTGGC 6 0.15 No Hit TGAGAGCCGAATATATAAGTAAGTACACTGTTGGGTGTCCTCTTGTGTCG 6 0.15 No Hit GTCTGCATGCGTTCATTCCATGATAATCGGAAATGCACTCTGCAGATTAA 6 0.15 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGT 6 0.15 No Hit GTCATCGTGGTGGTGGTGAGAGAGCATCAACAAGCATTGCTTCAACAAAA 6 0.15 No Hit TTTTACCTCATCTCATTCATAGTTCATAACACTAAACCTGTTGGCCATCA 6 0.15 No Hit GTTGATAGCATGAAAGATCCCAGCATTGCTGCATTTTGGTTGTTTTCCTT 6 0.15 No Hit AGGCAAGCCACATGCTGTTGAAACTGATTCGTCCATCAATGGAAATGGTC 6 0.15 No Hit GTGGTATGTACCCTGACTTGGGTTTCTCAGAGAGCACCACAACCGAGACA 6 0.15 No Hit CTGAAGCCTGTGGACGAATCCTTGGATCACTATAAGAAACAGCAATTGAG 6 0.15 No Hit TTTAGCTTCAGCAAATTCATTTTCCAAGTAGCCCTTTATCCTACTTTTCC 5 0.125 No Hit ACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTAC 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGT 5 0.125 No Hit TTTACTGAAGTCGACAATTACAAGTCTTCAAGGCAAGTTGCCATCCGAGT 5 0.125 No Hit GAGGAGGCACGTGAAAAATACAGAAGAAAAGAAAAGAAAAGAAAAGAAAG 5 0.125 No Hit TGTTCCTCAGAATGAGACTCCAGCTGTTGCTGATGCACCCGCACCCCTCG 5 0.125 No Hit CCTAATTTTTTCTCCCCTCACGGGGGGAGGCAGCCGCCAGCCCCCCACCG 5 0.125 No Hit CCTGAATTTTGATGAACAAGCTGTTCAATTCTTGATGAAATCTAATAATC 5 0.125 No Hit GGCAGTGATAAAGCCACTGATGGCCCTTCTGTTGGACCACGAGGTATCTA 5 0.125 No Hit GCCTTCCCATATATTTTGACATGCTTGTGGACCTGTGTAAGTTTGTTAGT 5 0.125 No Hit CACTTCCAACAGAGAGTGATGAGCATCCTAAGATCTACCGGATGAAGCTT 5 0.125 No Hit GGATCTTGCAAATTCTATTCAGAGGGGAAGTTTGGACTGGGAAAGAGCTT 5 0.125 No Hit CTGCCTTGGCACCAGAAATAGCTCCACCTGCACAAGCTGCTCTGCACCCT 5 0.125 No Hit GTTTTGCTCACAGGCATGGAAAGAGGTATGAGACAGAGGGGGAGATGAAG 5 0.125 No Hit GCCTTGGTGGGTTCTGCAGCTTCAAATAGCATAACCCTTGCTACACCACA 5 0.125 No Hit CGTCTACGTGGTGGCATGCAGATCTTTGTGAAGACCCTCACTGGAAAGAC 5 0.125 No Hit CGGGCAACAAGTTCCTGTATCTTCAAGACACTGCACATCAAGAACTCAGA 5 0.125 No Hit CAAACAAGGATCTCCAGCCAATAGCCAGGCTGCAAGCTATAGCATGCCAG 5 0.125 No Hit GCTGGATCGCCCTTTAGGTAGCTCAATGCCAAATAGTGCTGTTTCAACAC 5 0.125 No Hit GCCGGGAGGACAGAAGGGGGATTTCGATGAATTGGATGATTACGGTGGGT 5 0.125 No Hit AGAAGATTGTATCCGCTGTCTGTATAATGCTATGGCTAATCCATACATTT 5 0.125 No Hit GTTGATTAATGAGGAAAGTGCTAGTGAGCTACTTCAAGCTCAAACTCATG 5 0.125 No Hit CCAATGTTCTTTTACTTAGCATCGTCTTCTCTTCTTCATCTAGCCAGGAG 5 0.125 No Hit GAGGATAAGGCTCGGCTTCTGCAGTTTGTGACGGGCACATCCAAGGTGCC 5 0.125 No Hit AGGCGGAGAATTGTGACTGTCTTCAAGGATTTCAAGTTTGCCACTCACTC 5 0.125 No Hit GCGTTGGACAAAAACTCCAAGGAAGATGATGATGCTGGTGATTTTGCCAA 5 0.125 No Hit ACTCGGGAAGAATTTGATCGGTGCATTTTATCAACCTCAATGTGTGTTGA 5 0.125 No Hit CCTCACCTGTCTTCTATATTATAAACACACACCTCCGCAATCATCCTCTC 5 0.125 No Hit TTCATATCGGCTGCTTCAGTCTCTTGCCTCCAAGCCCCCAACTCGGGAAA 5 0.125 No Hit ACTACATCTCTGGCGATGAGCACAAGCTTGATTTAAGCACAACAAGGAGG 5 0.125 No Hit TCACAATTGTTCTAGCCTTTTGAGTAGTTCATCACATAGCTAGCTATGGC 5 0.125 No Hit GGGAAGTTTGACGGGCACGTGAGAACACTGGCATTTGCAAATTTCAGCGA 5 0.125 No Hit TTTCAGAACATATCAAGGGGGAAGAGAGAGAAGGCAAAGATCCGTGTCTC 5 0.125 No Hit CACAACTCCAAAATCAAATTCCCATTGGTATTCAAGAAGGTTAGACCCCC 5 0.125 No Hit CCAGGAACTGGCAGCCAAGGAGAGGGCTAGACCGGGATTCTGCCGACATG 5 0.125 No Hit TACATCGGCGACCTCGGTCTCATCCAATTCAAAGATCTCAATGCAGATAA 5 0.125 No Hit CATACACCCATGCTCTTGGCCTTGAGCTTGACCTGCAAGAGAAGGGACTT 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGT 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGT 5 0.125 No Hit AAGATGACTGGTGAAGTTGGTGAACAGGTGCAAATTGTTGGTGATGATCT 5 0.125 No Hit AGAAGACTCGTCATTATCTTCCAAACGGTTTCAAGAAATTTGTTGTCCAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.037500000000000006 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.0625 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.125 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.25 0.0 0.0 0.0 0.0 72-73 0.3 0.0 0.0 0.0 0.0 74-75 0.3 0.0 0.0 0.0 0.0 76-77 0.32499999999999996 0.0 0.0 0.0 0.0 78-79 0.42500000000000004 0.0 0.0 0.0 0.0 80-81 0.6125 0.0 0.0 0.0 0.0 82-83 0.8 0.0 0.0 0.0 0.0 84-85 0.825 0.0 0.0 0.0 0.0 86-87 0.9625 0.0 0.0 0.0 0.0 88-89 1.15 0.0 0.0 0.0 0.0 90-91 1.2875 0.0 0.0 0.0 0.0 92-93 1.5625 0.0 0.0 0.0 0.0 94-95 2.1 0.0 0.0 0.0 0.0 96-97 2.4875 0.0 0.0 0.0 0.0 98-99 3.2249999999999996 0.0 0.0 0.0 0.0 100-101 3.6125 0.0 0.0 0.0 0.0 102-103 4.175 0.0 0.0 0.0 0.0 104-105 4.8375 0.0 0.0 0.0 0.0 106-107 5.1875 0.0 0.0 0.0 0.0 108-109 5.55 0.0 0.0 0.0 0.0 110-111 6.0125 0.0 0.0 0.0 0.0 112-113 6.637499999999999 0.0 0.0 0.0 0.0 114-115 7.4625 0.0 0.0 0.0 0.0 116-117 8.1625 0.0 0.0 0.0 0.0 118-119 8.8625 0.0 0.0 0.0 0.0 120-121 9.575 0.0 0.0 0.0 0.0 122-123 10.462499999999999 0.0 0.0 0.0 0.0 124-125 11.15 0.0 0.0 0.0 0.0 126-127 11.8875 0.0 0.0 0.0 0.0 128-129 13.025 0.0 0.0 0.0 0.0 130-131 13.837499999999999 0.0 0.0 0.0 0.0 132-133 14.5625 0.0 0.0 0.0 0.0 134-135 15.5125 0.0 0.0 0.0 0.0 136-137 16.775 0.0 0.0 0.0 0.0 138-139 17.775 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATTGA 15 1.1411342E-4 145.0 3 AAGACCG 10 0.006830828 145.0 3 GACCGAC 10 0.006830828 145.0 5 GGGATTG 10 0.006830828 145.0 2 GCTTGCT 10 0.006830828 145.0 145 ATTGAAG 15 1.1411342E-4 145.0 5 TTGAAGA 15 1.1411342E-4 145.0 6 TGGGATT 10 0.006830828 145.0 1 CCGACAG 10 0.006830828 145.0 7 GATTGAA 20 3.5877043E-4 108.75 4 TGAAGAA 20 3.5877043E-4 108.75 7 TTTTGGG 25 4.977651E-4 29.0 50-54 AGGGGGG 25 4.977651E-4 29.0 75-79 GGGGGGA 25 4.977651E-4 29.0 65-69 GGGGGAA 30 0.0014437955 24.166668 65-69 TTTGGGG 30 0.0014437955 24.166668 50-54 AAAAAAA 75 1.5980913E-7 19.333334 120-124 GGGGGTT 40 0.0076550315 18.125 45-49 GGGGGGT 90 0.0048656333 11.277777 40-44 >>END_MODULE Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655963 spots for SRR26075402.sra Written 655963 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra Read 655956 spots for SRR26075402.sra Written 655956 spots for SRR26075402.sra SRR ids: ['SRR26075402.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_mh79yj3d SRR26075402.sra spots: 13119127 blocks: [[1, 655956], [655957, 1311912], [1311913, 1967868], [1967869, 2623824], [2623825, 3279780], [3279781, 3935736], [3935737, 4591692], [4591693, 5247648], [5247649, 5903604], [5903605, 6559560], [6559561, 7215516], [7215517, 7871472], [7871473, 8527428], [8527429, 9183384], [9183385, 9839340], [9839341, 10495296], [10495297, 11151252], [11151253, 11807208], [11807209, 12463164], [12463165, 13119127]] SRR26075402 file size 4837913 SRR26075402 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075402 SRR26075402_1.fastq SRR26075402_2.fastq Input file: SRR26075402_1.fastq Paired file: SRR26075402_2.fastq trimmed: SRR26075402-trimmed-pair1.fastq, SRR26075402-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:22:17 2025 >> started Wed Feb 12 01:22:31 2025 >> done (14.250s) 13119127 read pairs processed; of these: 242 ( 0.00%) short read pairs filtered out after trimming by size control 923492 ( 7.04%) empty read pairs filtered out after trimming by size control 12195393 (92.96%) read pairs available; of these: 2990027 (24.52%) trimmed read pairs available after processing 9205366 (75.48%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 7 0.00% 19 7 0.00% 20 7 0.00% 21 11 0.00% 22 13 0.00% 23 8 0.00% 24 9 0.00% 25 19 0.00% 26 20 0.00% 27 17 0.00% 28 27 0.00% 29 22 0.00% 30 24 0.00% 31 35 0.00% 32 37 0.00% 33 50 0.00% 34 54 0.00% 35 66 0.00% 36 61 0.00% 37 141 0.00% 38 196 0.00% 39 109 0.00% 40 251 0.00% 41 150 0.00% 42 142 0.00% 43 167 0.00% 44 161 0.00% 45 125 0.00% 46 206 0.00% 47 204 0.00% 48 282 0.00% 49 290 0.00% 50 314 0.00% 51 413 0.00% 52 665 0.01% 53 408 0.00% 54 663 0.01% 55 825 0.01% 56 654 0.01% 57 805 0.01% 58 1322 0.01% 59 999 0.01% 60 1390 0.01% 61 1194 0.01% 62 1408 0.01% 63 1954 0.02% 64 1672 0.01% 65 1856 0.02% 66 1874 0.02% 67 2004 0.02% 68 2362 0.02% 69 2745 0.02% 70 2981 0.02% 71 3644 0.03% 72 4020 0.03% 73 4647 0.04% 74 5234 0.04% 75 5403 0.04% 76 5771 0.05% 77 6522 0.05% 78 6960 0.06% 79 7717 0.06% 80 8110 0.07% 81 9363 0.08% 82 10594 0.09% 83 11966 0.10% 84 12978 0.11% 85 13904 0.11% 86 14710 0.12% 87 15488 0.13% 88 15924 0.13% 89 17146 0.14% 90 18278 0.15% 91 19671 0.16% 92 20483 0.17% 93 22602 0.19% 94 23958 0.20% 95 25233 0.21% 96 26289 0.22% 97 27413 0.22% 98 27765 0.23% 99 28534 0.23% 100 30077 0.25% 101 31104 0.26% 102 32028 0.26% 103 33927 0.28% 104 35878 0.29% 105 36287 0.30% 106 37754 0.31% 107 38201 0.31% 108 38579 0.32% 109 39710 0.33% 110 39333 0.32% 111 41724 0.34% 112 41445 0.34% 113 42507 0.35% 114 44089 0.36% 115 44856 0.37% 116 45612 0.37% 117 47263 0.39% 118 47884 0.39% 119 47435 0.39% 120 47463 0.39% 121 48338 0.40% 122 49233 0.40% 123 50521 0.41% 124 51215 0.42% 125 52440 0.43% 126 53039 0.43% 127 54284 0.45% 128 54151 0.44% 129 54215 0.44% 130 54849 0.45% 131 54671 0.45% 132 54490 0.45% 133 55804 0.46% 134 55599 0.46% 135 56514 0.46% 136 57593 0.47% 137 57951 0.48% 138 58852 0.48% 139 59150 0.49% 140 59167 0.49% 141 58566 0.48% 142 59753 0.49% 143 59247 0.49% 144 59797 0.49% 145 60359 0.49% 146 60446 0.50% 147 61641 0.51% 148 61471 0.50% 149 61071 0.50% 150 60691 0.50% 151 9205366 75.48% 12195393 reads passed initial QC criterion=sequence-density sequence-density=0.49 sequence-density-rank=1 fanout-score=29.24 fanout-score-rank=4 prefix-density=0.48 prefix-fanout=29.2 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCAACCATCTCGTATGCCGTCTTCTGCTTG criterion=fanout-score sequence-density=0.02 sequence-density-rank=36 fanout-score=165.98 fanout-score-rank=1 prefix-density=0.33 prefix-fanout=10.6 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.47 sequence-density-rank=1 fanout-score=2.64 fanout-score-rank=24 prefix-density=0.47 prefix-fanout=2.6 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.10 sequence-density-rank=20 fanout-score=88.07 fanout-score-rank=1 prefix-density=0.49 prefix-fanout=17.8 sequence=GAAGAAGAGAGG SRR26075402 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:23:15 Started mapping on | Feb 12 01:23:16 Finished on | Feb 12 01:25:50 Mapping speed, Million of reads per hour | 285.09 Number of input reads | 12195393 Average input read length | 287 UNIQUE READS: Uniquely mapped reads number | 10436101 Uniquely mapped reads % | 85.57% Average mapped length | 285.58 Number of splices: Total | 9443427 Number of splices: Annotated (sjdb) | 9200952 Number of splices: GT/AG | 9264748 Number of splices: GC/AG | 136509 Number of splices: AT/AC | 9625 Number of splices: Non-canonical | 32545 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 3.04 Insertion rate per base | 0.02% Insertion average length | 2.51 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 334989 % of reads mapped to multiple loci | 2.75% Number of reads mapped to too many loci | 41121 % of reads mapped to too many loci | 0.34% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 10.04% % of reads unmapped: other | 1.30% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1424303 1424303 1424303 N_multimapping 334989 334989 334989 N_noFeature 303032 10307287 383721 N_ambiguous 107041 669 58474 UnstrandedReadsAssigned:10026028 PositiveStrandReadsAssigned:128145 NegativeStrandReadsAssigned:9993906 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=141 echo kmer=137 SRR26075402 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075402-trimmed-pair1.fastq SRR26075402-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 12,195,393 reads, 10,242,001 reads pseudoaligned [quant] estimated average fragment length: 197.327 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,134 rounds 52401 SRR26075402.ke.tsv 34699 SRR26075402.se.tsv 87100 total ==> SRR26075402.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1821.67 1444 70.9762 Potri.005G024800.1.v4.1 1035 838.673 1942 207.335 Potri.004G059700.1.v4.1 961 764.673 1 0.117095 Potri.007G009000.2.v4.1 1416 1219.67 0 0 Potri.003G141000.2.v4.1 2943 2746.67 561 18.2882 Potri.016G087400.1.v4.1 270 103.037 856 743.866 Potri.015G069301.1.v4.1 564 369.619 0 0 Potri.010G195200.1.v4.1 1773 1576.67 436 24.7606 Potri.012G127500.1.v4.1 977 780.673 17398 1995.47 ==> SRR26075402.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 2 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 118 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 468 SRR26075402 completed mapping pipeline successfully