Starting /dee2/code/volunteer_pipeline.sh SRR26075403 current disk space = 3050778337280 free memory = 1520849140 SRR26075403 SRAfilesize 1085bcbfba15bf2e28ff08a3c698771e SRR26075403.sra SRR26075403.sra file validated SRR26075403 is paired end SRR26075403 is conventional basespace SRR26075403 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075403_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.64575 37.0 37.0 37.0 37.0 37.0 2 36.6695 37.0 37.0 37.0 37.0 37.0 3 36.6605 37.0 37.0 37.0 37.0 37.0 4 36.6935 37.0 37.0 37.0 37.0 37.0 5 36.71 37.0 37.0 37.0 37.0 37.0 6 36.7435 37.0 37.0 37.0 37.0 37.0 7 36.6765 37.0 37.0 37.0 37.0 37.0 8 36.674 37.0 37.0 37.0 37.0 37.0 9 36.6895 37.0 37.0 37.0 37.0 37.0 10-14 36.61505000000001 37.0 37.0 37.0 37.0 37.0 15-19 36.5793 37.0 37.0 37.0 37.0 37.0 20-24 36.5527 37.0 37.0 37.0 37.0 37.0 25-29 36.464 37.0 37.0 37.0 37.0 37.0 30-34 36.387 37.0 37.0 37.0 37.0 37.0 35-39 36.3167 37.0 37.0 37.0 37.0 37.0 40-44 36.1896 37.0 37.0 37.0 37.0 37.0 45-49 35.7047 37.0 37.0 37.0 37.0 37.0 50-54 35.7628 37.0 37.0 37.0 37.0 37.0 55-59 35.218900000000005 37.0 37.0 37.0 37.0 37.0 60-64 35.2678 37.0 37.0 37.0 37.0 37.0 65-69 35.20210000000001 37.0 37.0 37.0 37.0 37.0 70-74 35.571 37.0 37.0 37.0 37.0 37.0 75-79 35.9758 37.0 37.0 37.0 37.0 37.0 80-84 35.9871 37.0 37.0 37.0 37.0 37.0 85-89 35.854499999999994 37.0 37.0 37.0 37.0 37.0 90-94 35.8052 37.0 37.0 37.0 37.0 37.0 95-99 35.8433 37.0 37.0 37.0 37.0 37.0 100-104 35.737199999999994 37.0 37.0 37.0 37.0 37.0 105-109 35.6816 37.0 37.0 37.0 37.0 37.0 110-114 35.670700000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.486900000000006 37.0 37.0 37.0 37.0 37.0 120-124 35.547799999999995 37.0 37.0 37.0 37.0 37.0 125-129 35.4225 37.0 37.0 37.0 37.0 37.0 130-134 35.2847 37.0 37.0 37.0 34.6 37.0 135-139 35.1838 37.0 37.0 37.0 29.8 37.0 140-144 35.0472 37.0 37.0 37.0 29.8 37.0 145-149 35.016 37.0 37.0 37.0 25.0 37.0 150-151 34.72575 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 2.0 17 1.0 18 3.0 19 3.0 20 3.0 21 5.0 22 3.0 23 4.0 24 13.0 25 7.0 26 8.0 27 16.0 28 19.0 29 33.0 30 37.0 31 44.0 32 71.0 33 211.0 34 152.0 35 392.0 36 2771.0 37 202.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.56981699674104 17.6736024066182 8.698922035597894 30.057658561042867 2 17.625 19.45 32.875 30.049999999999997 3 16.275000000000002 18.55 33.525 31.65 4 20.075000000000003 21.675 26.125 32.125 5 24.2 28.725 26.450000000000003 20.625 6 25.374999999999996 30.599999999999998 25.124999999999996 18.9 7 16.425 32.625 36.0 14.95 8 14.45 32.725 31.05 21.775 9 20.674999999999997 24.2 33.550000000000004 21.575 10-14 18.437765664849728 33.00495074261139 26.80402060309046 21.75326298944842 15-19 19.28 30.220000000000002 27.32 23.18 20-24 18.84 31.355 27.155 22.650000000000002 25-29 19.045 30.56 26.87 23.525 30-34 18.15 31.019999999999996 27.465 23.365 35-39 19.255 30.3 28.59 21.855 40-44 18.505 29.555 27.439999999999998 24.5 45-49 19.52 30.014999999999997 27.279999999999998 23.185 50-54 19.445 28.63 27.315 24.610000000000003 55-59 20.415 28.444999999999997 27.555000000000003 23.585 60-64 20.815 28.355000000000004 27.889999999999997 22.939999999999998 65-69 20.474999999999998 30.685000000000002 26.674999999999997 22.165000000000003 70-74 22.905 28.46 26.424999999999997 22.21 75-79 23.435 28.325 25.900000000000002 22.34 80-84 22.93 28.07 26.16 22.84 85-89 23.22 28.18 25.655 22.945 90-94 23.615 27.97 25.540000000000003 22.875 95-99 23.865 28.21 25.96 21.965 100-104 23.915 27.66 26.055 22.37 105-109 24.165 27.689999999999998 25.869999999999997 22.275 110-114 24.235 27.92 24.875 22.97 115-119 23.665 27.935 25.165 23.235 120-124 24.615000000000002 26.82 25.19 23.375 125-129 25.005 27.1 24.975 22.919999999999998 130-134 24.11 27.6 24.240000000000002 24.05 135-139 24.93 26.435 25.095 23.54 140-144 25.224999999999998 27.49 23.825 23.46 145-149 25.509999999999998 26.169999999999998 24.474999999999998 23.845 150-151 25.724999999999998 25.874999999999996 23.674999999999997 24.725 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 0.5 2 0.5 3 0.5 4 1.0 5 1.0 6 0.5 7 1.0 8 1.0 9 1.0 10 1.0 11 0.5 12 0.0 13 1.0 14 1.5 15 1.5 16 2.0 17 1.0 18 0.0 19 0.0 20 1.0 21 2.5 22 5.0 23 5.0 24 5.0 25 8.5 26 14.5 27 17.0 28 15.5 29 32.0 30 41.0 31 46.0 32 61.5 33 62.0 34 80.0 35 116.5 36 116.0 37 116.5 38 139.0 39 162.5 40 196.5 41 215.5 42 219.0 43 234.5 44 231.0 45 221.5 46 214.5 47 187.5 48 164.0 49 144.5 50 132.0 51 129.0 52 94.5 53 68.0 54 75.0 55 52.5 56 34.5 57 29.0 58 28.5 59 24.5 60 15.0 61 14.0 62 13.0 63 11.0 64 9.0 65 14.5 66 26.5 67 38.5 68 36.0 69 24.0 70 18.0 71 11.5 72 4.5 73 3.0 74 1.5 75 0.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27499999999999997 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.015 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.45 #Duplication Level Percentage of deduplicated Percentage of total 1 64.55004205214466 38.375 2 21.068124474348192 25.05 3 7.359125315391085 13.125 4 3.8267451640033645 9.1 5 1.5138772077375946 4.5 6 0.7569386038687973 2.7 7 0.4625735912531539 1.925 8 0.2523128679562658 1.2 9 0.0 0.0 >10 0.16820857863751051 1.4000000000000001 >50 0.0 0.0 >100 0.04205214465937763 2.625 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTAGTCCATCTCGTAT 105 2.625 TruSeq Adapter, Index 22 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTAGTCCATCTCGTTT 21 0.525 TruSeq Adapter, Index 22 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTAGTCCATCGCGTAT 15 0.375 TruSeq Adapter, Index 22 (97% over 37bp) TTCCTAGATAGTGTTCTCTGTTTGAATGCTTCCATTGTTGAATAGACACC 10 0.25 No Hit GTTTGTTTAATACCCGTCTCTCTTATTTACTATACTGAAATACTGTTCAT 10 0.25 No Hit CCCTTGAGTTCTTGTAATCCCTCTTGACCAGAATACGGAAGATGCCTCTT 8 0.2 No Hit ATTGTTCCTTCAGCAACTCCAGTTGCTAGTGAAATATCTCGGAGAGGCTT 8 0.2 No Hit GCCTGGATCTCTTTTTTGCTAAGCTCTCACTACGGCGCTCACGCTGCTCC 8 0.2 No Hit ATTGCAACCGCCCCTCGAAAGCATTACAAGGCTAAATACCCCCATGTGAC 8 0.2 No Hit AAGGCCTCTGTTACGGGGAAATCAAAAACCACATCTTTTCCTGTAAGCTT 8 0.2 No Hit GACTGATTGAAACTAGTAGCTCCACAATTTACAAGCACTAAGCGGCGGAT 8 0.2 No Hit AGGCTATTTTCCCTTGGCATTTATCTCTCCCAACGTCACAGCGTCCACAT 7 0.17500000000000002 No Hit GTCGCAGTTACCAACAGCAACACCCATGCAAAGGACTTTCTTTAATTGAA 7 0.17500000000000002 No Hit CTCGCATTTGCAGTGGGTGGTGGCAGGTTGGCTAACGTTGCATTTTGCGT 7 0.17500000000000002 No Hit GCTCCATCCTGATTACATACTGGATTGGCAGTCCTGTAATCCTGTTTTGA 7 0.17500000000000002 No Hit TGAACAAATCTAGGCAGTACCCTCAACCAGCTTGTTCAACTTTTTTTGTC 7 0.17500000000000002 No Hit CTCATCTGGAAGTGGAGGAGGAGCATCCTCAGGTGGAGGTGGTGGAGGCA 7 0.17500000000000002 No Hit CAAAAGGTTCGGAGGAACGGAAGAAACGGGGTCCGACTTGACGGCCTGGG 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTAGTCCATCTCGGAT 7 0.17500000000000002 TruSeq Adapter, Index 22 (97% over 37bp) CAATTTTGGAAGCTATAAATAGCAAAATACCAAATTAAATCTTGGTTTGT 7 0.17500000000000002 No Hit GTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACA 7 0.17500000000000002 No Hit CCCGTCTCCGATTCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTAT 7 0.17500000000000002 No Hit GCTAAGGGCTTCGTCGATGTCTTTAGTCATATGAACCATAAGATCAACAT 6 0.15 No Hit CCATCGCCTTGCTTGAAATCCCAATATCTGGATGAACTTGTTTCAGGACC 6 0.15 No Hit CCCATTTCCATCCAGTTTGAAAGTCTAACAAAATCATGAAATTTAAAACG 6 0.15 No Hit CTTGTTTCTGTGGGAGATTGGGATGAAACAAAAGTATAGATGAGAATCAT 6 0.15 No Hit CCACCGAAAAAGTACAGTAGGCTTCCATATTTTTATTCAGAATCTATCAG 6 0.15 No Hit TCATCATTTTGCATTAGAACTTTTACACGTGTTCTAACTTCACATTTTGA 6 0.15 No Hit CAGTGCAGATGAAGTTCACAAGTTCTGAAAACTGACAAAGCAGCTAGTGA 6 0.15 No Hit GGCCTAACCCTATGGCTCAACACCATTTCCCTAAGTTCAGCTGGATCAAT 6 0.15 No Hit GTCTAAAGGATCGATAGGCCACGCTTTCACGGTTCGTATTCGTACTGGAA 6 0.15 No Hit ATCATGTGGAGAGCCTCTGAGTGGAAAGGAGAGTTATCTCCGACAAATAC 6 0.15 No Hit GTTGGTGCCCGAACTTTTCCACTCTTGATTGCAATGTTAAGATCTTCTGG 6 0.15 No Hit GTTAGTGTGTGGTCCTCATCAATCTGCTGCATGATCCTCAACTGTTTCTC 6 0.15 No Hit GTCCCAATGCCTAACCTGCAAACATGACTATTCCATAGTAGGCGTGACGC 6 0.15 No Hit GCCAGCACCATTTCCTTTGGGGTTATTGGGATCGACACCAGGCCATTCAC 6 0.15 No Hit CAGAGAACAAGCAGTAAAAAATTTCCAGTATCCGGTAACCACAGTATACA 6 0.15 No Hit GACATTCTTTCCAGCAACTTCAACAGCATCACACACCTTGTTAAATCCAT 6 0.15 No Hit GAGGATGTGAAGGGGAATTGTTGAAAGAGAAAAGATGGGATTTTGAATGG 6 0.15 No Hit AAGGCAGATAAGAAGAGCAAAATTCGTTTGTTATTCTCGTCATCAAACAT 6 0.15 No Hit CCCTGAACACGTTGAACAATATTGAAGGAAGAGTAAAGAAAATGTAGGGG 5 0.125 No Hit GAGCTTATTCTCTACCGGATCTGAGCCCACTTGAAGAGTTTCCAGTAAAT 5 0.125 No Hit GTTTTCAATATCATCATTGTTGACGGTGATTTCTTGAGTGGAACTTGCAT 5 0.125 No Hit CCAACATAAAACTTAGTCAAACAGACTGAAACCCATGTCATCATCAGACT 5 0.125 No Hit CCTTCAGCTTCACACTTCTTAGATGCATAACGATAACAGGAGAGACATCC 5 0.125 No Hit CTGATTGTATGACCTTTCCCAATTCTGCCCATCAAAGTTCTTAATCTCAA 5 0.125 No Hit GCCCATCCAAAAAACCTGTGTCCTTCGAATTTATGAGGGCGAAAAAGGTT 5 0.125 No Hit ATTGGCACAAAATCATCAGCAACCAGACGAGGATATTGGCTCTGATTAAA 5 0.125 No Hit GTTTCTGAGAGATTTCAGCACAGTCTTGTACCCCAGGGTGTATTTCCCAC 5 0.125 No Hit TTTTTTTAGATGTAGAAATAGGTAACATTTTATTTATACAGTAAGAAACG 5 0.125 No Hit GGATTGTGGGATACCAGTGGGACAATGCAGGAGTTGAAAGATCAAGCAGT 5 0.125 No Hit GGGCAGCAGAGGTTCATTATCCTTGGAGAGACTTAGAAGAGATAGTAGTC 5 0.125 No Hit CTCCAGTTATAATTACAGCACATCAGCACATACCATGAAGATTACGTTTG 5 0.125 No Hit GCATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCG 5 0.125 No Hit CGATGGAACTTTAAGTAGTAGCTAATCAAAATATTACTGTAGGTAAACAA 5 0.125 No Hit CACGTCAACAATCTTCTGACAAAGGCTTCTTCCCTGTAGAAACCACGTGC 5 0.125 No Hit AACCTCATCTAGCATGTCAACATCCAAAACACTAGGAATGATCCCAGCAC 5 0.125 No Hit CTGTTGTAATTTCATGGATGACAGATGTGGCTAAGTGTAAATAGAGTCCC 5 0.125 No Hit GCCTAAATCGATCTATTGACAACTAGGAGAAGAAATTCCGAACTAGACAC 5 0.125 No Hit GACCATGTTTCAACACATATTACGATAAATATAAACCATTTATACATTGC 5 0.125 No Hit GTACACCACAACCTGCTGAAAAGATGCAAATATTTACAAGAAAGAACCCC 5 0.125 No Hit GTAGCCGATGCCATATCCCACAAAGGTAATGGAGATTGAGGTCCTTGGGG 5 0.125 No Hit GGTAGTTTTTATTGGATCAACAAGAAACAGGCAACCTATCGTCTCGATGT 5 0.125 No Hit ATGGATCTGGCCTCGTGCAGGCTGTCTGGTTGCCAAGTGATATGGCTGGT 5 0.125 No Hit ATTCATGATAAGTGCTTTCTCATTAGCTCCTGCTTTAGGGAAGAAGCAAT 5 0.125 No Hit GCCGGTGTTATTTACATTACTTAACCACAACACAAAACAATACAATACAC 5 0.125 No Hit AGATGAAAGGGTTAGATATGGCAGAGATTGGATCAAGGAAAAGCTCTCCC 5 0.125 No Hit TCCTTAGAAACTTCCCTGAACTTTGATTGATGGTTGCTTCTACCGCCGAA 5 0.125 No Hit GCTTGTATGGACCCTCGATTGGCACGTTGATGTAGTCAGCCTGGTCCTTG 5 0.125 No Hit GGTAAGGGCATTCTTGAAGCTCTGAAGCAATTTTTCCATGAATTTTCTTG 5 0.125 No Hit AGGGTTTTCAACACATAAGGGCTCATCATGTAAAGCTGGACCTGGACATC 5 0.125 No Hit CAGCACATGATGGAGAGACCACAAGAAACAGCGAAAATAGTATGCAACCC 5 0.125 No Hit CTATGAAATACGAATGCCCCCGACTGTCCCTGTTAATCATTACTCCGATC 5 0.125 No Hit ATTCCCTCACAAGCTAGAGTGCCCCCAAGATCACCAATAATTCGAGCAAT 5 0.125 No Hit CTTCCATACATCAACAAGAAAATCCACCATTTCAGCAAGAGGGACAGGTC 5 0.125 No Hit CTTTAGATCTTGCTCTTGTGTTCAGGCCTATGGGAAAACTCGAAATCTAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.05 0.0375 0.0 0.0 0.0 60-61 0.075 0.075 0.0 0.0 0.0 62-63 0.125 0.075 0.0 0.0 0.0 64-65 0.1375 0.075 0.0 0.0 0.0 66-67 0.15 0.075 0.0 0.0 0.0 68-69 0.15 0.075 0.0 0.0 0.0 70-71 0.15 0.075 0.0 0.0 0.0 72-73 0.15 0.075 0.0 0.0 0.0 74-75 0.1875 0.075 0.0 0.0 0.0 76-77 0.225 0.075 0.0 0.0 0.0 78-79 0.2375 0.075 0.0 0.0 0.0 80-81 0.375 0.075 0.0 0.0 0.0 82-83 0.4375 0.075 0.0 0.0 0.0 84-85 0.5375000000000001 0.075 0.0 0.0 0.0 86-87 0.575 0.075 0.0 0.0 0.0 88-89 0.7875000000000001 0.075 0.0 0.0 0.0 90-91 0.8999999999999999 0.075 0.0 0.0 0.0 92-93 1.025 0.075 0.0 0.0 0.0 94-95 1.15 0.075 0.0 0.0 0.0 96-97 1.4125 0.075 0.0 0.0 0.0 98-99 1.6875 0.075 0.0 0.0 0.0 100-101 1.9375 0.075 0.0 0.0 0.0 102-103 2.25 0.075 0.0 0.0 0.0 104-105 2.6625 0.075 0.0 0.0 0.0 106-107 2.9625 0.075 0.0 0.0 0.0 108-109 3.1625 0.075 0.0 0.0 0.0 110-111 3.5125 0.075 0.0 0.0 0.0 112-113 3.9875 0.075 0.0 0.0 0.0 114-115 4.5375 0.075 0.0 0.0 0.0 116-117 5.012499999999999 0.075 0.0 0.0 0.0 118-119 5.8 0.075 0.0 0.0 0.0 120-121 6.512499999999999 0.075 0.0 0.0 0.0 122-123 7.1 0.075 0.0 0.0 0.0 124-125 7.7875000000000005 0.075 0.0 0.0 0.0 126-127 8.5625 0.075 0.0 0.0 0.0 128-129 9.1375 0.075 0.0 0.0 0.0 130-131 10.075 0.075 0.0 0.0 0.0 132-133 11.100000000000001 0.075 0.0 0.0 0.0 134-135 12.1875 0.075 0.0 0.0 0.0 136-137 13.1875 0.075 0.0 0.0 0.0 138-139 14.025 0.075 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACACAC 10 0.006830828 145.0 9 ACTGATT 10 0.006830828 145.0 2 CGCCTTG 10 0.006830828 145.0 5 AAATCAC 10 0.006830828 145.0 5 AATCACA 10 0.006830828 145.0 6 GATTGAA 10 0.006830828 145.0 5 TCGCCTT 10 0.006830828 145.0 4 TCTTGCC 10 0.006830828 145.0 145 CTTCGTC 10 0.006830828 145.0 145 ATCACAC 10 0.006830828 145.0 7 GTGTGTG 10 0.006830828 145.0 5 TCACACA 10 0.006830828 145.0 8 GAGCACA 90 1.021077E-6 48.333332 9 AAGAGCA 100 2.1114429E-6 43.5 7 GAAGAGC 100 2.1114429E-6 43.5 6 CGGAAGA 100 2.1114429E-6 43.5 4 AGAGCAC 100 2.1114429E-6 43.5 8 TCGGAAG 105 2.9547027E-6 41.42857 3 ATCGGAA 110 4.0695504E-6 39.545456 2 GGAAGAG 110 4.0695504E-6 39.545456 5 >>END_MODULE SRR26075403 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075403_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.297 37.0 37.0 37.0 37.0 37.0 2 36.258 37.0 37.0 37.0 37.0 37.0 3 36.3255 37.0 37.0 37.0 37.0 37.0 4 36.2655 37.0 37.0 37.0 37.0 37.0 5 36.349 37.0 37.0 37.0 37.0 37.0 6 36.2765 37.0 37.0 37.0 37.0 37.0 7 36.2585 37.0 37.0 37.0 37.0 37.0 8 36.139 37.0 37.0 37.0 37.0 37.0 9 36.1685 37.0 37.0 37.0 37.0 37.0 10-14 36.005399999999995 37.0 37.0 37.0 37.0 37.0 15-19 35.8823 37.0 37.0 37.0 37.0 37.0 20-24 35.7028 37.0 37.0 37.0 37.0 37.0 25-29 35.3852 37.0 37.0 37.0 37.0 37.0 30-34 35.03 37.0 37.0 37.0 32.2 37.0 35-39 34.90259999999999 37.0 37.0 37.0 32.2 37.0 40-44 34.814600000000006 37.0 37.0 37.0 25.0 37.0 45-49 34.6957 37.0 37.0 37.0 25.0 37.0 50-54 34.4071 37.0 37.0 37.0 25.0 37.0 55-59 34.564699999999995 37.0 37.0 37.0 25.0 37.0 60-64 34.7564 37.0 37.0 37.0 25.0 37.0 65-69 34.5673 37.0 37.0 37.0 25.0 37.0 70-74 34.3538 37.0 37.0 37.0 25.0 37.0 75-79 34.267399999999995 37.0 37.0 37.0 25.0 37.0 80-84 34.3418 37.0 37.0 37.0 25.0 37.0 85-89 34.5542 37.0 37.0 37.0 25.0 37.0 90-94 34.6798 37.0 37.0 37.0 25.0 37.0 95-99 34.8806 37.0 37.0 37.0 25.0 37.0 100-104 34.894999999999996 37.0 37.0 37.0 27.4 37.0 105-109 35.0294 37.0 37.0 37.0 29.8 37.0 110-114 34.944 37.0 37.0 37.0 27.4 37.0 115-119 34.9759 37.0 37.0 37.0 27.4 37.0 120-124 34.8611 37.0 37.0 37.0 25.0 37.0 125-129 34.816199999999995 37.0 37.0 37.0 25.0 37.0 130-134 34.7958 37.0 37.0 37.0 25.0 37.0 135-139 34.66395 37.0 37.0 37.0 25.0 37.0 140-144 34.695499999999996 37.0 37.0 37.0 25.0 37.0 145-149 34.59915 37.0 37.0 37.0 25.0 37.0 150-151 34.248374999999996 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 15.0 14 23.0 15 26.0 16 12.0 17 9.0 18 9.0 19 10.0 20 19.0 21 14.0 22 23.0 23 33.0 24 29.0 25 37.0 26 48.0 27 49.0 28 23.0 29 19.0 30 17.0 31 28.0 32 39.0 33 49.0 34 120.0 35 470.0 36 2609.0 37 269.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.724862431215605 20.135067533766886 10.380190095047524 19.759879939969984 2 34.375 25.674999999999997 23.65 16.3 3 29.4 26.375 27.075 17.150000000000002 4 31.35 31.0 21.125 16.525000000000002 5 33.275 33.525 17.974999999999998 15.225 6 27.575 35.949999999999996 21.4 15.075 7 27.250000000000004 21.625 32.225 18.9 8 27.725 24.625 24.125 23.525 9 29.65 23.3 26.275 20.775 10-14 30.94 27.055 22.915 19.09 15-19 29.915000000000003 27.025 24.25 18.81 20-24 29.835 27.575 23.785 18.805 25-29 30.055 26.445 23.794999999999998 19.705000000000002 30-34 29.64 27.384999999999998 23.925 19.05 35-39 29.39 27.015 24.295 19.3 40-44 29.544999999999998 27.229999999999997 23.56 19.665 45-49 28.884999999999998 26.245 25.4 19.470000000000002 50-54 23.385 26.61 29.830000000000002 20.175 55-59 27.284999999999997 26.400000000000002 26.845000000000002 19.470000000000002 60-64 29.025000000000002 26.889999999999997 24.48 19.605 65-69 28.555000000000003 27.08 25.895000000000003 18.47 70-74 26.455000000000002 28.439999999999998 26.400000000000002 18.705 75-79 24.315 30.425 26.25 19.009999999999998 80-84 28.02 27.465 25.72 18.795 85-89 27.825 26.584999999999997 26.755000000000003 18.834999999999997 90-94 28.075 26.815 25.509999999999998 19.6 95-99 27.92 27.3 25.81 18.970000000000002 100-104 28.535 28.249999999999996 25.52 17.695 105-109 28.175 26.674999999999997 26.87 18.279999999999998 110-114 28.92 27.355 25.495 18.23 115-119 28.465 26.790000000000003 26.145000000000003 18.6 120-124 27.872787278727873 27.677767776777678 26.122612261226124 18.326832683268325 125-129 28.395 27.57 26.35 17.685000000000002 130-134 29.86 26.845000000000002 26.44 16.855 135-139 29.212303075768943 26.281570392598148 26.731682920730183 17.774443610902726 140-144 30.32713085234094 27.70608243297319 25.55522208883553 16.41156462585034 145-149 30.01250938203653 27.685764323242434 25.54916187140355 16.752564423317487 150-151 30.39889958734526 27.447792922345883 26.172314617981744 15.980992872327123 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 1.0 10 0.0 11 0.0 12 1.5 13 2.0 14 1.0 15 2.0 16 2.0 17 2.0 18 1.5 19 3.5 20 4.0 21 2.5 22 3.0 23 2.5 24 2.0 25 2.5 26 3.5 27 4.5 28 8.5 29 11.0 30 8.5 31 9.0 32 13.5 33 19.5 34 26.5 35 32.0 36 42.5 37 86.5 38 121.5 39 130.0 40 150.0 41 203.5 42 259.5 43 275.0 44 248.5 45 247.0 46 230.0 47 206.0 48 227.5 49 207.0 50 156.5 51 129.5 52 126.5 53 121.0 54 88.5 55 60.0 56 55.5 57 45.0 58 34.0 59 24.0 60 19.0 61 21.5 62 16.5 63 11.5 64 10.5 65 8.0 66 8.5 67 3.5 68 3.0 69 2.5 70 3.0 71 3.0 72 2.0 73 2.5 74 2.5 75 2.5 76 2.5 77 5.5 78 5.0 79 3.0 80 5.5 81 7.5 82 12.5 83 13.5 84 14.5 85 13.5 86 11.5 87 15.0 88 17.5 89 17.5 90 13.0 91 10.5 92 8.0 93 6.5 94 5.0 95 1.5 96 4.0 97 6.0 98 4.0 99 3.5 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.01 125-129 0.0 130-134 0.0 135-139 0.025 140-144 0.04 145-149 0.075 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.9 #Duplication Level Percentage of deduplicated Percentage of total 1 65.84564860426929 40.1 2 20.77175697865353 25.3 3 6.773399014778326 12.375 4 3.6535303776683086 8.9 5 1.6830870279146142 5.125 6 0.5747126436781609 2.1 7 0.28735632183908044 1.225 8 0.24630541871921183 1.2 9 0.0 0.0 >10 0.08210180623973727 0.5 >50 0.08210180623973727 3.175 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 68 1.7000000000000002 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 59 1.4749999999999999 No Hit CAAGCTCAAACACCATCATCATCATCCCCACTTAAGTATAAAGGCATTCT 10 0.25 No Hit GGTTCTATGGCAGCCCTTTTGGATCTCCAAAGCTCTGATCCTGATCCTCA 10 0.25 No Hit TCTAGGATTCTGAATCAAAAGAAAGAAGGAACACAGAGCTTGGATACTTT 8 0.2 No Hit GGGACATTTACCGGGACGAATCATTGGACTACAAGAATACTGTTGCTGCT 8 0.2 No Hit CTGGGTTTGGAGGCAGGTCAGTCTGTGGAGATGGGAACTATACATGCTGG 8 0.2 No Hit GGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAA 8 0.2 No Hit CTGAGATTGTTGGAAAACGCACCAGATACAGGATTGATGGGTCCAAAATT 8 0.2 No Hit CTTTCATCTACTTTACATTGGAGACACTCAATTTCCTAATCTTCAAAGGA 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT 7 0.17500000000000002 No Hit AGTTCATCGACCTTTTGTCCCTCTTCATGTACCCCAACCCGGATTGCCTA 7 0.17500000000000002 No Hit GGAGAACCTGTCATTGACACGGGTGATTGGAGAATTCAATTGCAGCCTGA 7 0.17500000000000002 No Hit AAGGGCTACCCCGGTATCTCGAGGCACAAGAAGAGCTCCCCGGTGATGAC 7 0.17500000000000002 No Hit TGGAAGTCCAAAAGTACTTAATTGAACATCCAGAGTATATGACATATGGT 7 0.17500000000000002 No Hit GTTTCCACTTGGCTATCTTGCCAAAAACTTCCTTGAAAAGAGGGAGATGT 7 0.17500000000000002 No Hit TCAGAGTCTGTCATCAAGCAGATTCCTCGACTTTTAGGCCCTGGTCTCAA 7 0.17500000000000002 No Hit GGATTTCAGACAAAAAGAAGAGAATTGCAAAGGCCAAGTCAGAAGCAGCT 6 0.15 No Hit AAAAGGAGAAGTTGGAACACAGGTACTGAGTTCAGGCATGCAGTAGGGAT 6 0.15 No Hit GTTTCAGCGAGGGTGTATCACAATTCACATGGCACGAGTTGCTTTGTTCT 6 0.15 No Hit GGCTGATTCAGCTATTGCGAACAATGCTATCTTGAGGTTGATTGCTGGAA 6 0.15 No Hit GCCAAAGGCAGGCAAGAAGCTCCCAAAAGAAGGCGGAGCAGCAGTAGCTG 6 0.15 No Hit GCAGAGTAGCATGTGGTGTTATTGGTTTGCAAGGCTGAACTACCTCACTC 6 0.15 No Hit GCAGAAGTTCCTGAGTCGGACATAGCTCAAGCATGTAGAGGATTGCTTGA 6 0.15 No Hit CCAAGGAGTTGATATACATGCAGGTGACTGGGAGACTGCGGGCTCTATCA 6 0.15 No Hit GGAAGACATTGTCAGGTGGGGAGTTTGGCTGGGGCGGCACATCTGTTAAA 6 0.15 No Hit GAGGGAGAAAGGGGTTTAGCTTTAGCAAGGCAGGCAGAAAATGGAGAGTG 6 0.15 No Hit GATTCATTCATATATAGTTTTACCCATTCAAATGTAGTCAACTTTAGTGT 6 0.15 No Hit ATGTGAACTTCCTGGTTGATGTCCATATTCGAGCTTTCCAGGACCGCTCC 6 0.15 No Hit TGTTAGGCATGTTCTTCTTAGACAGGGAGTGCTTGGTATCAAGGTCAAGA 6 0.15 No Hit GGATCATCTCTTTTTCAGATAATCAGGACAGCATCTAGTGGTGGGATGGC 6 0.15 No Hit GAGATTTTGGCCCAAGATCCAACTGCTCCTGTAACTCTAACTTTGAATGA 5 0.125 No Hit GCGTGGTTCCGCCCCACCAGCCTTGAGGACCTGGAGAAGCCACACCGTCT 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGT 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGT 5 0.125 No Hit AAAAAAGTCAAGCAAATCGAACAATTTTTGGAGCACTAGTGCTGGTGATA 5 0.125 No Hit GAACCTAAAGAAAAATGCCCTCTATGCCGGAAGACCGGAGTTTACGAAGG 5 0.125 No Hit GCTCCTCTTCAATGATGGAGATGCAGTTGGGTATGGAGACCCTCTTATAG 5 0.125 No Hit GTGGGAAGGATGATTTTGTCTCACTTGTGTGATGAACCGAAGGGATTGAA 5 0.125 No Hit GTACTCATCTGCAATATGTCTCTTGTTGTTATGGTTCTACGGTTCTACCG 5 0.125 No Hit CTTCATTTTGTCTGCAGAAACCTCTCGCATAACTACCATGGTGGCCGCCA 5 0.125 No Hit GGGAGAAACTAAAACATTAGAGCAGTTCCTAGAGGAAACATTTCAGGTTC 5 0.125 No Hit CCCTGAACGGATGGAAAAGAAGAAAGGTTACTTGGCGATGAGGACGGATC 5 0.125 No Hit GAAACACACAACCTCTACGATGTCAAATTCTTCGATGACAGGATCCACAC 5 0.125 No Hit GAACGAGAGGAAAACCGGCAAGTACGAGAGGAAGGTCTATGTCTTGCCCA 5 0.125 No Hit GCTGTGACATTGAATACAAATATCCTGTGCATTCTTCACAGACTTATATC 5 0.125 No Hit TAATCAGAGATCCATGGATGGTGCCAGGGACTCAGAAATAGCCATGGGAG 5 0.125 No Hit AGCAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCATAGGATTT 5 0.125 No Hit AATGGGTGCAAGATCGAGTGGTCTTTCATTGCTGATCCAGTTGAAGGGTG 5 0.125 No Hit TGTGAATTCTGTGTTAGGGTTTGAGTCAATGAAAGGGGTTGAAATGCAGC 5 0.125 No Hit GTTCATCAATGCCTACAAGAATGTTCTGGCTGTTGCAGTTGCAACAGAGT 5 0.125 No Hit CTGAAAGAATGTCGAGTGGTGGCACAAAGTTTACTTCTGGGCTTCAAAGA 5 0.125 No Hit CCATATATTCAATGATATCGATAGTGATGATGGTGCAGGAAGGGAAGAAA 5 0.125 No Hit ATGTAAGATCCTCGACACCTTTGTGTGGGAAAACCAAGTAGGTTTAGTCA 5 0.125 No Hit CCTTATTGTCATCCAGATTGAGAATCTTAACAACAAATATGGGTGCAGCG 5 0.125 No Hit AAGGACTGTGGGAAAGTCAAAGCTGGTGGTGCCTACACTTTGAACACTGC 5 0.125 No Hit GATGAAGATACTGGAACTGAAAAGGGAAAGGAAGGATGGAACTGCGATGG 5 0.125 No Hit CAGCTTCCTTGGTGGTGTGACTCAACCATGGTTTCCTCAAGATCTAGCGA 5 0.125 No Hit TTTAAAAAACCACATCTTTGAGAGCCCTCTCCTCTTTCGCTTTTACTCTC 5 0.125 No Hit TATAGGATCAAAGATGATCTGGATTTTTCTGTTGAGATATATGAATGGTC 5 0.125 No Hit GTGATGTGAACACACTAGAATAAACTTTGATCAAGAGTGTAAATGTCTGG 5 0.125 No Hit TGGGATTTCGTGGAAGAGTGACCGAGAAACATAAAGGAGCCCTTGTAAAT 5 0.125 No Hit TACAAGAGGAAATGCAGAACCCTACACATCAACCTTTTCTGTTAAATGAT 5 0.125 No Hit TACAGAATCTGCTCTCCGATCGGTGTAATCTACCACCTACCAGTACTTAC 5 0.125 No Hit GAGGAGCATGAGCATGGGCATGGACACGAGCATGGGCATGGGCATGGGCA 5 0.125 No Hit GCAAGTTGCAGGCATAACTAGTAGTGATCTTGAAGGCATAAGTACTGATA 5 0.125 No Hit ATTCCAAAGAATTTCTTACAACCTACACTTTTGGCACGCATACAGCGAAG 5 0.125 No Hit GGTTATCCGTGGCTTCTCGCAACCAGTATAGCCTTGAAAATCGACGAGAA 5 0.125 No Hit GCTAAAAGTGGTGGTGAGATGGAGTCTGCTCAAGCAAAAAATCAAGCACT 5 0.125 No Hit GGTTGCTTAAACTACATTGAGGAAAATGATGAGGTTTTGATTGCTGGATT 5 0.125 No Hit CAAGCACATTGACACAGTTAGGGCATACACTTTCGGTAACCATTACTTTG 5 0.125 No Hit GGGAATTTTCTGGTGTGGAGATGTGACTGTGGATAGGTTGAAGTGGGGTA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.11249999999999999 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.16249999999999998 0.0 0.0 0.0 0.0 80-81 0.325 0.0 0.0 0.0 0.0 82-83 0.3875 0.0 0.0 0.0 0.0 84-85 0.4875 0.0 0.0 0.0 0.0 86-87 0.525 0.0 0.0 0.0 0.0 88-89 0.75 0.0 0.0 0.0 0.0 90-91 0.875 0.0 0.0 0.0 0.0 92-93 1.0 0.0 0.0 0.0 0.0 94-95 1.125 0.0 0.0 0.0 0.0 96-97 1.3875000000000002 0.0 0.0 0.0 0.0 98-99 1.6625 0.0 0.0 0.0 0.0 100-101 1.9125 0.0 0.0 0.0 0.0 102-103 2.2375 0.0 0.0 0.0 0.0 104-105 2.6625 0.0 0.0 0.0 0.0 106-107 2.9625 0.0 0.0 0.0 0.0 108-109 3.2 0.0 0.0 0.0 0.0 110-111 3.5625 0.0 0.0 0.0 0.0 112-113 4.0375 0.0 0.0 0.0 0.0 114-115 4.5875 0.0 0.0 0.0 0.0 116-117 5.0625 0.0 0.0 0.0 0.0 118-119 5.875 0.0 0.0 0.0 0.0 120-121 6.6625 0.0 0.0 0.0 0.0 122-123 7.275 0.0 0.0 0.0 0.0 124-125 7.975 0.0 0.0 0.0 0.0 126-127 8.725000000000001 0.0 0.0 0.0 0.0 128-129 9.3 0.0 0.0 0.0 0.0 130-131 10.2875 0.0 0.0 0.0 0.0 132-133 11.4375 0.0 0.0 0.0 0.0 134-135 12.5625 0.0 0.0 0.0 0.0 136-137 13.55 0.0 0.0 0.0 0.0 138-139 14.412500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTCAT 10 0.006830828 145.0 145 TTACCGG 10 0.006830828 145.0 8 GCAGGCA 10 0.006830828 145.0 8 GGACATT 10 0.006830828 145.0 2 CATTTAC 10 0.006830828 145.0 5 ACATTTA 10 0.006830828 145.0 4 GACATTT 10 0.006830828 145.0 3 TACCGGG 10 0.006830828 145.0 9 GCTCGGG 10 0.006830828 145.0 8 GGCTCGG 10 0.006830828 145.0 7 AGGCTCG 10 0.006830828 145.0 6 AAGGCTC 10 0.006830828 145.0 5 AAAGGCT 10 0.006830828 145.0 4 AAAGGCA 10 0.006830828 145.0 4 GCTTGTA 10 0.006830828 145.0 145 GGGACAT 10 0.006830828 145.0 1 CTCGGGC 10 0.006830828 145.0 9 TTTACCG 10 0.006830828 145.0 7 GGCAGGC 10 0.006830828 145.0 7 CAAAAGG 10 0.006830828 145.0 2 >>END_MODULE Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635944 spots for SRR26075403.sra Written 1635944 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra Read 1635939 spots for SRR26075403.sra Written 1635939 spots for SRR26075403.sra SRR ids: ['SRR26075403.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_4t7s31xg SRR26075403.sra spots: 32718785 blocks: [[1, 1635939], [1635940, 3271878], [3271879, 4907817], [4907818, 6543756], [6543757, 8179695], [8179696, 9815634], [9815635, 11451573], [11451574, 13087512], [13087513, 14723451], [14723452, 16359390], [16359391, 17995329], [17995330, 19631268], [19631269, 21267207], [21267208, 22903146], [22903147, 24539085], [24539086, 26175024], [26175025, 27810963], [27810964, 29446902], [29446903, 31082841], [31082842, 32718785]] SRR26075403 file size 12081844 SRR26075403 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075403 SRR26075403_1.fastq SRR26075403_2.fastq Input file: SRR26075403_1.fastq Paired file: SRR26075403_2.fastq trimmed: SRR26075403-trimmed-pair1.fastq, SRR26075403-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:45:22 2025 >> started Wed Feb 12 01:46:16 2025 >> done (54.174s) 32718785 read pairs processed; of these: 314 ( 0.00%) short read pairs filtered out after trimming by size control 1277185 ( 3.90%) empty read pairs filtered out after trimming by size control 31441286 (96.10%) read pairs available; of these: 6287979 (20.00%) trimmed read pairs available after processing 25153307 (80.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 19 0.00% 19 25 0.00% 20 28 0.00% 21 39 0.00% 22 37 0.00% 23 40 0.00% 24 58 0.00% 25 75 0.00% 26 54 0.00% 27 79 0.00% 28 68 0.00% 29 84 0.00% 30 71 0.00% 31 59 0.00% 32 70 0.00% 33 90 0.00% 34 59 0.00% 35 84 0.00% 36 81 0.00% 37 85 0.00% 38 85 0.00% 39 114 0.00% 40 99 0.00% 41 151 0.00% 42 171 0.00% 43 150 0.00% 44 174 0.00% 45 177 0.00% 46 184 0.00% 47 235 0.00% 48 233 0.00% 49 335 0.00% 50 297 0.00% 51 300 0.00% 52 373 0.00% 53 433 0.00% 54 422 0.00% 55 495 0.00% 56 505 0.00% 57 574 0.00% 58 678 0.00% 59 662 0.00% 60 774 0.00% 61 891 0.00% 62 972 0.00% 63 1102 0.00% 64 1337 0.00% 65 1273 0.00% 66 1573 0.01% 67 1680 0.01% 68 1800 0.01% 69 2116 0.01% 70 2368 0.01% 71 2773 0.01% 72 3245 0.01% 73 4109 0.01% 74 4335 0.01% 75 4745 0.02% 76 5144 0.02% 77 5871 0.02% 78 6673 0.02% 79 7264 0.02% 80 8228 0.03% 81 9490 0.03% 82 10875 0.03% 83 12227 0.04% 84 13770 0.04% 85 14883 0.05% 86 16370 0.05% 87 17604 0.06% 88 19779 0.06% 89 20189 0.06% 90 22749 0.07% 91 24620 0.08% 92 26918 0.09% 93 30993 0.10% 94 32894 0.10% 95 35687 0.11% 96 37523 0.12% 97 40847 0.13% 98 42677 0.14% 99 43773 0.14% 100 46526 0.15% 101 48920 0.16% 102 51538 0.16% 103 55564 0.18% 104 58524 0.19% 105 61520 0.20% 106 64322 0.20% 107 67461 0.21% 108 69627 0.22% 109 71511 0.23% 110 73318 0.23% 111 76144 0.24% 112 79883 0.25% 113 82453 0.26% 114 86334 0.27% 115 89507 0.28% 116 91526 0.29% 117 93606 0.30% 118 98300 0.31% 119 98278 0.31% 120 100959 0.32% 121 102713 0.33% 122 105713 0.34% 123 109803 0.35% 124 112663 0.36% 125 116481 0.37% 126 120214 0.38% 127 124032 0.39% 128 123600 0.39% 129 126162 0.40% 130 128699 0.41% 131 130293 0.41% 132 132715 0.42% 133 133779 0.43% 134 138038 0.44% 135 139958 0.45% 136 141696 0.45% 137 142767 0.45% 138 145623 0.46% 139 148890 0.47% 140 149536 0.48% 141 150281 0.48% 142 150533 0.48% 143 154027 0.49% 144 159144 0.51% 145 160693 0.51% 146 160684 0.51% 147 163809 0.52% 148 166780 0.53% 149 166020 0.53% 150 166619 0.53% 151 25153307 80.00% 31441286 reads passed initial QC criterion=sequence-density sequence-density=0.43 sequence-density-rank=1 fanout-score=32.90 fanout-score-rank=3 prefix-density=0.43 prefix-fanout=32.9 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTAGTCCATCTCGTATGCCGTCTTCTGCTTG criterion=fanout-score sequence-density=0.10 sequence-density-rank=34 fanout-score=163.29 fanout-score-rank=1 prefix-density=0.95 prefix-fanout=16.4 sequence=TCATCATCACCACCATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTC criterion=sequence-density sequence-density=0.49 sequence-density-rank=1 fanout-score=2.83 fanout-score-rank=31 prefix-density=0.55 prefix-fanout=2.5 sequence=TATTGGTGATGGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=95.89 fanout-score-rank=1 prefix-density=0.23 prefix-fanout=5.8 sequence=ATTTTCTTTGAGAGTGCATAGATTTGTGTTGATATAGAAAACAATGGCACTACATGGAAAGATTGAGACAACATTAGAACTCAAGTCCTCCGCAGAGAAGTTCTACAAAGTGTGGAGGAGCCAGTCCTTCCATGTTCCCAAACATGCTTCCAAGCATATCCAAGGAGTTGATATACATGCAGGTGACTGGGAGACTGCGGGCTCTATCAGGATTTGGCAGTACACAATCGGAGGGAAAGCCGGGGTCTTTAAAGAGGAGGTTTCCTTCGATGATGAGAACAAGATCATAACTCTTAATGGTTTGGAAGGAGATGTCATGAAAATTTACAAGGTCTATAGGCCCGTCTGGCAGCTTACACCAAAAGGCTCGGGCTGCTTGGCAAAACTGACCATTGAATACGAAAAACTCCATCCTGAAGTCCCGGTTCCAGAGATTTATGTTGATCTTATGGTTCATATGACTAAAGACATCGACGAAGCCCTTAGCACGGAGTAATAGAAGGGGTCATCG SRR26075403 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:47:10 Started mapping on | Feb 12 01:47:11 Finished on | Feb 12 01:52:49 Mapping speed, Million of reads per hour | 334.88 Number of input reads | 31441286 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 26388932 Uniquely mapped reads % | 83.93% Average mapped length | 289.99 Number of splices: Total | 18940385 Number of splices: Annotated (sjdb) | 18336041 Number of splices: GT/AG | 18558756 Number of splices: GC/AG | 268920 Number of splices: AT/AC | 22037 Number of splices: Non-canonical | 90672 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.05% Deletion average length | 3.50 Insertion rate per base | 0.03% Insertion average length | 2.44 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1006659 % of reads mapped to multiple loci | 3.20% Number of reads mapped to too many loci | 532339 % of reads mapped to too many loci | 1.69% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 10.03% % of reads unmapped: other | 1.14% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4045695 4045695 4045695 N_multimapping 1006659 1006659 1006659 N_noFeature 857067 26003263 1016558 N_ambiguous 380774 2088 153393 UnstrandedReadsAssigned:25151091 PositiveStrandReadsAssigned:383581 NegativeStrandReadsAssigned:25218981 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=149 echo kmer=145 SRR26075403 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075403-trimmed-pair1.fastq SRR26075403-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 31,441,286 reads, 26,478,936 reads pseudoaligned [quant] estimated average fragment length: 197.78 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,261 rounds 52401 SRR26075403.ke.tsv 34699 SRR26075403.se.tsv 87100 total ==> SRR26075403.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1821.22 2562 34.6783 Potri.005G024800.1.v4.1 1035 838.22 3158 92.8743 Potri.004G059700.1.v4.1 961 764.22 16 0.516111 Potri.007G009000.2.v4.1 1416 1219.22 0 0 Potri.003G141000.2.v4.1 2943 2746.22 869 7.80056 Potri.016G087400.1.v4.1 270 93.7729 4162 1094.12 Potri.015G069301.1.v4.1 564 367.503 0 0 Potri.010G195200.1.v4.1 1773 1576.22 395 6.17763 Potri.012G127500.1.v4.1 977 780.22 25553 807.358 ==> SRR26075403.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 117 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 1019 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 112 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1046 SRR26075403 completed mapping pipeline successfully