Starting /dee2/code/volunteer_pipeline.sh SRR26075404 current disk space = 3050874929152 free memory = 1468536428 SRR26075404 SRAfilesize f54d12c357f14ae8938d538555c48581 SRR26075404.sra SRR26075404.sra file validated SRR26075404 is paired end SRR26075404 is conventional basespace SRR26075404 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075404_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.60825 37.0 37.0 37.0 37.0 37.0 2 36.59 37.0 37.0 37.0 37.0 37.0 3 36.647 37.0 37.0 37.0 37.0 37.0 4 36.68 37.0 37.0 37.0 37.0 37.0 5 36.711 37.0 37.0 37.0 37.0 37.0 6 36.709 37.0 37.0 37.0 37.0 37.0 7 36.6415 37.0 37.0 37.0 37.0 37.0 8 36.6375 37.0 37.0 37.0 37.0 37.0 9 36.6465 37.0 37.0 37.0 37.0 37.0 10-14 36.6562 37.0 37.0 37.0 37.0 37.0 15-19 36.57950000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.5651 37.0 37.0 37.0 37.0 37.0 25-29 36.51899999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.47840000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.4634 37.0 37.0 37.0 37.0 37.0 40-44 36.421800000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.332100000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.267700000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.22539999999999 37.0 37.0 37.0 37.0 37.0 60-64 36.21210000000001 37.0 37.0 37.0 37.0 37.0 65-69 36.1263 37.0 37.0 37.0 37.0 37.0 70-74 36.1265 37.0 37.0 37.0 37.0 37.0 75-79 36.031099999999995 37.0 37.0 37.0 37.0 37.0 80-84 35.993100000000005 37.0 37.0 37.0 37.0 37.0 85-89 35.9396 37.0 37.0 37.0 37.0 37.0 90-94 35.9067 37.0 37.0 37.0 37.0 37.0 95-99 35.9049 37.0 37.0 37.0 37.0 37.0 100-104 35.824400000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.818200000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.6833 37.0 37.0 37.0 37.0 37.0 115-119 35.5652 37.0 37.0 37.0 37.0 37.0 120-124 35.5276 37.0 37.0 37.0 37.0 37.0 125-129 35.5399 37.0 37.0 37.0 37.0 37.0 130-134 35.3991 37.0 37.0 37.0 37.0 37.0 135-139 35.3087 37.0 37.0 37.0 29.8 37.0 140-144 35.11579999999999 37.0 37.0 37.0 27.4 37.0 145-149 35.222300000000004 37.0 37.0 37.0 29.8 37.0 150-151 35.19125 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 4.0 23 2.0 24 3.0 25 5.0 26 7.0 27 13.0 28 20.0 29 22.0 30 27.0 31 34.0 32 70.0 33 95.0 34 183.0 35 438.0 36 2906.0 37 170.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.987722375344525 15.409671761463292 9.095464795790528 36.507141067401655 2 18.15 13.325000000000001 35.949999999999996 32.574999999999996 3 16.425 16.85 30.7 36.025 4 22.025 22.925 25.025 30.025000000000002 5 22.525000000000002 27.650000000000002 26.025 23.799999999999997 6 23.575 31.8 22.975 21.65 7 15.4 28.125 39.925 16.55 8 17.849999999999998 28.475 29.349999999999998 24.325 9 17.025000000000002 24.725 34.2 24.05 10-14 19.7 29.220000000000002 28.09 22.99 15-19 19.24 28.74 28.17 23.849999999999998 20-24 20.21 27.944999999999997 27.88 23.965 25-29 19.950000000000003 28.37 28.205000000000002 23.474999999999998 30-34 19.36 27.315 28.189999999999998 25.135 35-39 20.115 29.38 27.0 23.505000000000003 40-44 20.465 28.89 26.815 23.830000000000002 45-49 19.96 27.87 27.365000000000002 24.805 50-54 20.3 27.305 27.85 24.545 55-59 20.385 28.485 27.205000000000002 23.925 60-64 20.65 26.939999999999998 27.985 24.425 65-69 20.369999999999997 27.615000000000002 28.65 23.365 70-74 21.404999999999998 27.47 27.6 23.525 75-79 20.724999999999998 26.974999999999998 28.025 24.275 80-84 20.09 28.544999999999998 27.834999999999997 23.53 85-89 20.849999999999998 28.134999999999998 27.750000000000004 23.265 90-94 21.224999999999998 28.084999999999997 26.825 23.865 95-99 20.915 27.655 28.08 23.35 100-104 21.6 26.395000000000003 27.275 24.73 105-109 21.029999999999998 27.46 27.43 24.08 110-114 20.605 27.295 28.29 23.810000000000002 115-119 20.549999999999997 27.650000000000002 27.975 23.825 120-124 21.515 27.88 26.58 24.025 125-129 21.310000000000002 27.284999999999997 27.665 23.74 130-134 21.815 27.900000000000002 26.815 23.47 135-139 21.345 27.66 26.66 24.335 140-144 21.39 27.82 26.365 24.425 145-149 21.505 27.12 27.125 24.25 150-151 21.4 26.674999999999997 25.837500000000002 26.087500000000002 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 1.5 23 2.5 24 2.0 25 3.5 26 4.0 27 4.0 28 8.5 29 16.0 30 20.5 31 24.0 32 24.5 33 26.0 34 33.0 35 50.0 36 86.0 37 106.0 38 123.5 39 147.0 40 177.0 41 223.0 42 244.5 43 254.0 44 278.0 45 287.0 46 275.0 47 255.0 48 225.5 49 201.0 50 176.0 51 149.0 52 115.5 53 103.5 54 89.5 55 62.0 56 50.5 57 35.5 58 19.5 59 14.0 60 16.5 61 10.5 62 4.0 63 7.5 64 9.0 65 5.5 66 5.5 67 5.5 68 5.5 69 4.5 70 3.0 71 1.5 72 0.5 73 1.0 74 1.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.824999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 63.62515413070283 38.7 2 20.674064940402793 25.15 3 9.453349773941635 17.25 4 3.1237155774763665 7.6 5 1.7673653925195234 5.375 6 0.5754212905877518 2.1 7 0.4110152075626798 1.7500000000000002 8 0.08220304151253596 0.4 9 0.16440608302507193 0.8999999999999999 >10 0.12330456226880394 0.775 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGATGACATCTCGTAT 11 0.27499999999999997 TruSeq Adapter, Index 15 (97% over 36bp) CTCTGGTGGAACAAAGGGCCCTTTTGCAGCGGCAGCTACTGTAATGGAAG 10 0.25 No Hit CCCATGCTTCATATCCTTTGTTGAGTGCATCAGAGAATCCAGCAACAACA 10 0.25 No Hit AGCGCCTCACCCTCAAAGATACGGCGTGAGTTCTTCTCATCAAGGGTTAG 9 0.22499999999999998 No Hit ATTGGTTTTGTTCATTTCTAATACTGATACAAAATGAAAGTAGTGCACAC 9 0.22499999999999998 No Hit TTCTCACTTTTAACATCACTGCCACTATGGCAAGCATTGCCTACTACTGC 9 0.22499999999999998 No Hit TCAGCATCTGATACTCCGCTCCTTGATACCCGCAATTTTTCAATTTCTTT 9 0.22499999999999998 No Hit CTTGAATTCAATGTTGTTTGTGATGGTTGAGGGATTGGTGCAGAACGAGG 8 0.2 No Hit CCTCGTTATTGGCATACTTGTGAATCTCTTCCTCTTCTTTGATCTTGAAC 8 0.2 No Hit CTTGGATACAAAGGCACATATCGCTTATTTTTTAGCCCAACAACTCCAAA 7 0.17500000000000002 No Hit GCTTCTTTAACAGAGAGATGGGTAGAGAATTTTCCACGTGCATCAGGGCT 7 0.17500000000000002 No Hit CATGAAAACAAAAGGAGGGCTGCATCTAAAACAGCGATGTCCCCTTGCCC 7 0.17500000000000002 No Hit ATCACTTTATCTGGATCAACCACTTCAGGTATGGTCTCTCTAGTCTTCTT 7 0.17500000000000002 No Hit GCAAGATCGCAACACAAACATGATTAGAACAACATTAAGGACCAACCCAA 7 0.17500000000000002 No Hit AGACGCAGCTTTTCCCTTTGCAGGGTGGGGAGTTACTTGCCCAGCCTTCC 7 0.17500000000000002 No Hit CGTCTGCTTCGTTTCCAGGCCATGAAACGACTCCAAGTTCAAGATTTGCT 7 0.17500000000000002 No Hit GTGGCAGCAAAGTTGAACCATTCTCAGTAATTTCTGACAGAATAGACTGT 7 0.17500000000000002 No Hit TGTAAGCATGCATCGATCTGGCCTTGGGTCCTCGCACATGTATGAACACA 7 0.17500000000000002 No Hit AGCCAGTAACAGAATCAGGAAGACCATTTCTAGGTCGAGCAGCTCGTCTG 7 0.17500000000000002 No Hit CCTCGACTCCTTGTTCTCATTTTTATTTGCATTCTCCTCAGAACCAGTTT 6 0.15 No Hit CTCTTAACATGGTTCTCAAAATATACATGAAGAAGAGAACGATCATCATT 6 0.15 No Hit CCGCATTCTCATCCTCTGTGTCATCTTCATCATCATCGTCCATTCCAGGA 6 0.15 No Hit GCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGT 6 0.15 No Hit GTGGGCAACAGTGTAATAAGCCATGTAAATAGTGGAGTCAGATAATGACT 6 0.15 No Hit ACCATGTACCAATATATGACTGATCTCTTCATGCTTCTTGGGCAATCAAA 6 0.15 No Hit TCTCAGGCTCGGCAATCTGTTTTTCCATGTATTTTTTGCCAGTACCAAGG 6 0.15 No Hit GCCGGTTCGAAAACGATTGACAATGAGCAATGCCAATGGTATGAGCACCA 6 0.15 No Hit GATCCGTAGTGCACCAAAGCCACGAGCCATCTGCACCTTTACCTTTCTCT 6 0.15 No Hit TGGGCAATGAGCCAAGAAGTATATCCGATGCAGATATAGACTGGCTCCCA 6 0.15 No Hit CCATCTTCCACTCCTCTGTGGATGGATTCACCTTAAAATCAAAATCAACT 6 0.15 No Hit GCCCTCTTCCCCTTTCCAGACTTCTTCGAGCTCAGGAAAACCCTGGGCTG 6 0.15 No Hit CAGCTGCAGAAACGAAAACACCACCTAAGGCGTCCATCAGAGATGATGAT 6 0.15 No Hit CACCTTTATAACACACAGTCACGTCAACATTTACTATATCACCATCCTCT 6 0.15 No Hit TCTATCTATTGGGCATGCAATTGAAACATGTGGAAGTATCTCTAGGTTTC 5 0.125 No Hit GGTGCTTTCTCAGCCTCTGACAGTGACTTCCATTTATCTCCTCCAGCTTT 5 0.125 No Hit TCATCAAGTCCCAATGGCCTTAGATCTACAAGAATGAGATGATTGTCGCT 5 0.125 No Hit GTCACCTTGGTAACAAAGGAAAGCATGGGGTCCACAACCAGCTTGGTGAC 5 0.125 No Hit CTAACAACTTAGCCTTGATGTTTTTCTAAATCAAGTTATATCAGCAGCAA 5 0.125 No Hit GTAGAGATCTAAAGGCATCTAATCTTAACATAATCTGTGGAGAGATCACA 5 0.125 No Hit ATCCAGAGAGAACTAATACTTTAGCCAGCCACATTATGGACGGAAAATTG 5 0.125 No Hit CTTGTTAGGGATGTTTTTAATCATTAAAGTTGTCCTAGTATCTTCCCCGC 5 0.125 No Hit GCCCCCTTAAGCACGTTCACCTCTGATCCTCCTAGCCAGCTGGATATCCT 5 0.125 No Hit CCCCGTTCCATCCTTAACATCAATGAATCCATCTTGTTTGGTCTTTTCCA 5 0.125 No Hit GCATTCAATAGGTTCAAAACACAGCATAAACTTCTATCACAAACAGCGAC 5 0.125 No Hit CTGGTCAACTTCACCACTGAGCAACTTCATGTACCCACCACCACAGTCAA 5 0.125 No Hit CCCTTTTTAAGGATGTTAAGCACCTTGGTTCGCTTCTGAATGGGGGCCGC 5 0.125 No Hit ACACTAGAAGCACAGCATCCCTCAACTCATCCTCATTCAACATCCTGTGT 5 0.125 No Hit GTTCCGTAGACTTCAGCATCTTCTTATTCTGTAAGAGGAGCAAGAGAGAG 5 0.125 No Hit GATCAACATCCTCAAAAACAATGATTGGGCTTTTCCCACCTAGCTCCATT 5 0.125 No Hit TGGAAAATTTCTTGCCAATGCAAGCTCTAGGTCCGATTCCAAAGGGTATA 5 0.125 No Hit CCTCATCATCCTCCATGAATACTGGCAGACCTGCATAACCTTGATCAAAT 5 0.125 No Hit AACCAGAGCTATAAGTAGACACCCGTGGAGACCAACCATGAAACATCAAC 5 0.125 No Hit GTGGGCCAGGAGGATAACCAGATGGTGGGTATGCACCGGGAGGGTACCCA 5 0.125 No Hit GGTATCTGCTACAGGGACGCGTGTCTCCCACTCTGCATCTCTTTTTAGCC 5 0.125 No Hit GGCAGCGGTAGGGTCCATTATCTGGTGTTTCTTGGCACGGAGGAGATTAG 5 0.125 No Hit CCCATGAATACCGCATTTGCTAGAGCAAAACCCTTCAACAGCAACATCAG 5 0.125 No Hit AAGTGAAACAGAAATAGTGACTGCAGCAGTCCAGTGTTCCCCTGAATCTT 5 0.125 No Hit TTTGAAACAGCAGCTGACACTCCTCCCATGAGAAAATCAATCATAAATCC 5 0.125 No Hit CTATCTTCTTCACTGCTGGACCGATCAGCTTGGCAGTAAAATTCTGCAAT 5 0.125 No Hit AGGGAATGAGAGTGTGAGCGGGAGTGGGTGCGTGTTCGGGACCGGGATGG 5 0.125 No Hit GATGAGGACTATGAGGAGGAAGATTAGGATTCCGGCGAAGATTCGACGGA 5 0.125 No Hit GGATCATTCATGATTTTTTGGCACTTTTAGCAATGGAAGATGCAAGATAG 5 0.125 No Hit TGTGGATTTACCGTAAGAATTCAAGAGGGAATAGCAAACTGAGGAGCTCT 5 0.125 No Hit GCCAATACCAATGTGGACTAGTCCACCTAGGACATCCACACATGCGCCTA 5 0.125 No Hit CTTGTAAATTTTCATGACATCTCCTTCCAAACCATTAAGAGTTATGATCT 5 0.125 No Hit CCTCCTCCTAAAGTGCTCCTCTTTCTCCTCTTCTTTCCTCATTTCTGGCA 5 0.125 No Hit TAGACAGGACCTCGACCTCCTCCACACCTAATGCATGGAAGCACTGCAAG 5 0.125 No Hit CCTTTTCCTTGCAGAACTCAAGCATCTCCTCTGTCTCCTTCATGCTCCCT 5 0.125 No Hit AGACCCAACAACAAGCTTCCTATACAAATCCACATCATCCTTCTTCACAA 5 0.125 No Hit GTTTCTTGGTCACAGAGAGCTTGGCGCTCTTAGTATCTGACTTTGATCTA 5 0.125 No Hit TGCACTTGCTCGATGATGGAATTGGCTGTGCAAAAGCGACGGGCTTATTT 5 0.125 No Hit GTAACCTTCAAAAGCATAAGTTGGTTAAATCCAACATACAATGTTTCATC 5 0.125 No Hit GTTCAAAAAGGTAATGAATTTGATGAAAATAAGGAAAATTAAGATCTGGG 5 0.125 No Hit TCCAGGTCACAGTAGAAACCTTAAAGTCTCCAAGCATTAACAAAAAAGAT 5 0.125 No Hit CTCTTTTTATCATTTTTTGCTATAAAGGGATAATCTTTGAAACCCATTAC 5 0.125 No Hit GCTGTTGTAGCGGAAGAAACAACTCTGGAGGCCTCAAATCAAAGACTTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0375 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1375 0.0 0.0 0.0 0.0 76-77 0.1875 0.0 0.0 0.0 0.0 78-79 0.3 0.0 0.0 0.0 0.0 80-81 0.375 0.0 0.0 0.0 0.0 82-83 0.4375 0.0 0.0 0.0 0.0 84-85 0.475 0.0 0.0 0.0 0.0 86-87 0.625 0.0 0.0 0.0 0.0 88-89 0.7749999999999999 0.0 0.0 0.0 0.0 90-91 0.825 0.0 0.0 0.0 0.0 92-93 0.9625 0.0 0.0 0.0 0.0 94-95 1.1625 0.0 0.0 0.0 0.0 96-97 1.2 0.0 0.0 0.0 0.0 98-99 1.4874999999999998 0.0 0.0 0.0 0.0 100-101 1.6875 0.0 0.0 0.0 0.0 102-103 1.75 0.0 0.0 0.0 0.0 104-105 1.8875000000000002 0.0 0.0 0.0 0.0 106-107 2.175 0.0 0.0 0.0 0.0 108-109 2.3375 0.0 0.0 0.0 0.0 110-111 2.7125000000000004 0.0 0.0 0.0 0.0 112-113 2.9375 0.0 0.0 0.0 0.0 114-115 3.2375 0.0 0.0 0.0 0.0 116-117 3.825 0.0 0.0 0.0 0.0 118-119 4.275 0.0 0.0 0.0 0.0 120-121 4.575 0.0 0.0 0.0 0.0 122-123 5.0 0.0 0.0 0.0 0.0 124-125 5.325 0.0 0.0 0.0 0.0 126-127 5.8875 0.0 0.0 0.0 0.0 128-129 6.525 0.0 0.0 0.0 0.0 130-131 7.1625 0.0 0.0 0.0 0.0 132-133 7.9125 0.0 0.0 0.0 0.0 134-135 8.2125 0.0 0.0 0.0 0.0 136-137 8.537500000000001 0.0 0.0 0.0 0.0 138-139 9.05 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCATTT 10 0.006830828 145.0 3 GTTGAGC 10 0.006830828 145.0 145 CATTGGG 10 0.006830828 145.0 3 TTTCCAC 10 0.006830828 145.0 7 ATTGGGA 10 0.006830828 145.0 4 CATTTCC 10 0.006830828 145.0 5 TTGGGAA 10 0.006830828 145.0 5 TTCCACG 10 0.006830828 145.0 8 AAGGTCT 10 0.006830828 145.0 145 GAACAGA 10 0.006830828 145.0 9 GGGAACA 10 0.006830828 145.0 7 CTGCATT 10 0.006830828 145.0 2 TCATTGG 10 0.006830828 145.0 2 GGAACAG 10 0.006830828 145.0 8 >>END_MODULE SRR26075404 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075404_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3455 37.0 37.0 37.0 37.0 37.0 2 36.3095 37.0 37.0 37.0 37.0 37.0 3 36.3135 37.0 37.0 37.0 37.0 37.0 4 36.3245 37.0 37.0 37.0 37.0 37.0 5 36.422 37.0 37.0 37.0 37.0 37.0 6 36.2865 37.0 37.0 37.0 37.0 37.0 7 36.2305 37.0 37.0 37.0 37.0 37.0 8 36.337 37.0 37.0 37.0 37.0 37.0 9 36.3815 37.0 37.0 37.0 37.0 37.0 10-14 36.3672 37.0 37.0 37.0 37.0 37.0 15-19 36.3019 37.0 37.0 37.0 37.0 37.0 20-24 36.267399999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.1978 37.0 37.0 37.0 37.0 37.0 30-34 36.042 37.0 37.0 37.0 37.0 37.0 35-39 36.009299999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.018100000000004 37.0 37.0 37.0 37.0 37.0 45-49 35.9092 37.0 37.0 37.0 37.0 37.0 50-54 35.7843 37.0 37.0 37.0 37.0 37.0 55-59 35.843399999999995 37.0 37.0 37.0 37.0 37.0 60-64 35.8609 37.0 37.0 37.0 37.0 37.0 65-69 35.765100000000004 37.0 37.0 37.0 37.0 37.0 70-74 35.6606 37.0 37.0 37.0 37.0 37.0 75-79 35.5766 37.0 37.0 37.0 37.0 37.0 80-84 35.641799999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.673899999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.5413 37.0 37.0 37.0 37.0 37.0 95-99 35.642900000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.488 37.0 37.0 37.0 37.0 37.0 105-109 35.5084 37.0 37.0 37.0 37.0 37.0 110-114 35.4711 37.0 37.0 37.0 37.0 37.0 115-119 35.401599999999995 37.0 37.0 37.0 37.0 37.0 120-124 35.226800000000004 37.0 37.0 37.0 32.2 37.0 125-129 35.307 37.0 37.0 37.0 37.0 37.0 130-134 35.256299999999996 37.0 37.0 37.0 34.6 37.0 135-139 35.1539 37.0 37.0 37.0 29.8 37.0 140-144 35.108 37.0 37.0 37.0 29.8 37.0 145-149 35.0561 37.0 37.0 37.0 27.4 37.0 150-151 34.83925 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 1.0 14 8.0 15 7.0 16 4.0 17 5.0 18 0.0 19 2.0 20 6.0 21 10.0 22 6.0 23 18.0 24 6.0 25 13.0 26 11.0 27 16.0 28 16.0 29 25.0 30 25.0 31 35.0 32 41.0 33 78.0 34 156.0 35 579.0 36 2684.0 37 247.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.85 21.325 11.175 24.65 2 29.549999999999997 26.200000000000003 26.924999999999997 17.325 3 22.825 28.125 30.175 18.875 4 24.075 34.275 23.825 17.825 5 27.325 33.225 20.599999999999998 18.85 6 21.85 37.824999999999996 23.150000000000002 17.175 7 22.175 22.15 37.6 18.075 8 23.75 26.400000000000002 24.525 25.324999999999996 9 24.075 25.775 28.999999999999996 21.15 10-14 25.230000000000004 29.375 25.535000000000004 19.86 15-19 25.005 27.744999999999997 26.88 20.369999999999997 20-24 24.349999999999998 28.505000000000003 26.790000000000003 20.355 25-29 25.28 28.060000000000002 25.629999999999995 21.029999999999998 30-34 25.25 28.73 25.135 20.885 35-39 24.45 28.660000000000004 26.490000000000002 20.4 40-44 25.285000000000004 28.58 26.479999999999997 19.655 45-49 24.64 28.395 26.68 20.285 50-54 23.825 28.59 26.715 20.87 55-59 25.369999999999997 27.755000000000003 25.64 21.235 60-64 24.8 28.444999999999997 25.990000000000002 20.765 65-69 25.41 28.255000000000003 26.33 20.005 70-74 24.94 28.299999999999997 26.46 20.3 75-79 24.240000000000002 28.87 26.279999999999998 20.61 80-84 24.73 29.060000000000002 26.205000000000002 20.005 85-89 25.0 27.725 26.905 20.369999999999997 90-94 24.779999999999998 28.895 25.905 20.419999999999998 95-99 25.005 28.025 26.515 20.455000000000002 100-104 24.54 27.96 27.065 20.435 105-109 24.635 28.555000000000003 26.945000000000004 19.865 110-114 25.25 27.955000000000002 27.3 19.495 115-119 25.27 28.444999999999997 26.435 19.85 120-124 24.495 29.134999999999998 26.63 19.74 125-129 25.72 28.144999999999996 26.855 19.28 130-134 25.28 28.435 26.38 19.905 135-139 26.46 28.77 25.985000000000003 18.785 140-144 26.83 29.175 24.98 19.015 145-149 26.384999999999998 27.425 26.665 19.525000000000002 150-151 28.025 27.987499999999997 25.2 18.787499999999998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 1.0 3 2.0 4 1.0 5 0.5 6 0.5 7 0.5 8 0.5 9 0.5 10 1.0 11 1.0 12 1.0 13 0.5 14 0.0 15 0.0 16 1.0 17 1.0 18 0.5 19 0.5 20 0.0 21 1.0 22 2.0 23 2.0 24 3.5 25 2.5 26 1.0 27 4.5 28 5.5 29 8.5 30 16.0 31 19.0 32 16.5 33 11.0 34 26.0 35 51.5 36 57.5 37 82.0 38 110.5 39 136.5 40 162.0 41 215.5 42 256.0 43 257.0 44 265.5 45 293.0 46 293.5 47 250.0 48 247.0 49 224.0 50 172.5 51 156.0 52 140.5 53 114.0 54 80.0 55 55.5 56 50.0 57 33.5 58 24.0 59 18.5 60 12.0 61 12.5 62 13.0 63 7.0 64 2.5 65 9.0 66 8.0 67 4.0 68 4.0 69 1.5 70 1.0 71 2.0 72 2.0 73 1.0 74 1.0 75 0.5 76 0.5 77 0.5 78 1.0 79 1.0 80 0.5 81 1.5 82 1.5 83 2.5 84 3.0 85 2.0 86 2.0 87 2.5 88 2.5 89 1.5 90 2.0 91 2.0 92 2.5 93 2.0 94 0.5 95 1.5 96 1.0 97 0.5 98 2.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.95 #Duplication Level Percentage of deduplicated Percentage of total 1 64.52009844134537 39.324999999999996 2 19.9343724364233 24.3 3 9.064807219031994 16.575 4 3.281378178835111 8.0 5 1.6817063166529942 5.125 6 0.6972928630024611 2.55 7 0.4922067268252666 2.1 8 0.04101722723543889 0.2 9 0.12305168170631665 0.675 >10 0.16406890894175555 1.15 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 15 0.375 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 11 0.27499999999999997 No Hit CTGGCATCGATACGAACTTCACTGTCACAGCAAAAGCAGCCGAAGATACA 10 0.25 No Hit CTTTATTTCTGATGATGGGAAATATGGGGAGTCGAGTGACTTGAGAGCAC 10 0.25 No Hit TCCATCAAGCAGAATCAACTCATTTCAATGCATCAGACACTTTTAAGTTC 9 0.22499999999999998 No Hit GAGGAAGAGGTAGTACCAGCAACAGCCGCATCCCATTCAGACGGAGGCCT 9 0.22499999999999998 No Hit GACAAAGTGACAGCACATACCAAGGAATTCATCCGAAAACAGTTAGAGAA 9 0.22499999999999998 No Hit TGTCATCGGTGGTTCTTCCAGAATGCCCACTCCTTCTCCTTCTCAGACTT 8 0.2 No Hit CTTTGTGCCAGTTTATGTGGCAATAGGGATGATAGCTGTATCAATATCAC 7 0.17500000000000002 No Hit CCAAAGACTTGCATGGAGTTGAATGGAGATTTCGTCATATTTACAGAGGT 7 0.17500000000000002 No Hit GCTGTCTCGTCAGATGGCTCATCACATGCTCCAAGATGATGATCATGAAA 7 0.17500000000000002 No Hit CAAGGTCACACAGTACAAGAAGGGGAAGGATAGCCTAGCTGCTCAAGGAA 7 0.17500000000000002 No Hit GCAAAGCAAAGCATTACTGTATCTCCCAAGTAAGCAGTGAAGATGTCTCA 7 0.17500000000000002 No Hit CTATGAGAAGGAACGTTTGGATGCTGAGCTGAGGCTCGTCGGAGAGTATG 7 0.17500000000000002 No Hit GATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGT 7 0.17500000000000002 No Hit GGCCAAATGATTTGCTCTCACTAATTGTTGGTGGTGAAAAAGGTGGATCC 7 0.17500000000000002 No Hit CTCACACGTAAAAATATCCTCCCCCCTTCTCTCCTCCTCTTTCTCTCTCT 7 0.17500000000000002 No Hit CGTGGGAGAAAACTGAAGTATAGACAAAGGGAGAGGAAGAGAGGCAGGCG 7 0.17500000000000002 No Hit CACCACCAACGTCGGAGGCGGCGCCACCACCACCACCAAAGTCGGAGGCG 7 0.17500000000000002 No Hit TGGAGGGTTGTCAGTGAACGGCTTTTCCATGGGTTGAAAACACAATACTC 7 0.17500000000000002 No Hit GTGAAATTGCAAGATATGAAAGCAGCCTCTCATGATGATCCTACCAGATG 6 0.15 No Hit AGGATTGCCGGAACGTGCTGTTTCCGTTCTTCGCGCCTGGCTCTTTGAGC 6 0.15 No Hit GGGTTTTTCAACTTGCACTCCTCTCTCAGCAAAGCAGCAGCAGCACATCA 6 0.15 No Hit GCTTCCATACCACACTGCCACTGCCTCCGCATTGCTTAATTCAATGCTCT 6 0.15 No Hit GGAAGAACTTCAAGCAGAAGTTGGATTGCCTGTTGACAGAGATATTCCTG 6 0.15 No Hit CTCAACTCTCGCTCTCTCTCTGCCTTTCACTATCAGGAAAAAAAAAATGC 6 0.15 No Hit GATTTACGTGCTGTAATCGAACACTTCCGCGGAGCAAGCCCAAGCAGGGG 6 0.15 No Hit GAGGACTTAGAGGAAGTCGAGCAACATCCCTCGTTTCTAACAACTCTCCA 6 0.15 No Hit GTTTGTTGGTGATGGTTATACTCGCTGTGAAGCTTCGGGTTCTTTACGTT 6 0.15 No Hit TGTAAGTGCAGCCGTTGGCATCACTGGACGTGCATTTGAAGCTGATTTAC 6 0.15 No Hit CTGCAATAAAGACACCAGTGTCCTCAAAGAAGGCTAAAACTGCTACCCCT 6 0.15 No Hit CATTCGCCCTGGTGTGACAACAGATGAAATTGACAGAGTTGTTCATGAGG 6 0.15 No Hit TAATTATCTTCTCTCCCCTTCTCGCCCTTTCTCCATCTCTAGACATGGGT 6 0.15 No Hit GGGAAAGATAGACGTGGAAAAACAGAGAGAGAGAGAGAGAGAGAGAGAGA 6 0.15 No Hit AAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGC 6 0.15 No Hit GGAATTGGATCTTCACTTACCATACAGTTGCCCTCAAATTGTCATCAGCT 6 0.15 No Hit AAGGCTGAGAATAAATGGTGGAGCAGATTGATAAAACAAACCGGAAAACC 6 0.15 No Hit CGAGCCTTACCTTTCTCTATTGAAACTTGATGGCAAGCTGATCTTGATGG 5 0.125 No Hit ACTAAGTTCACTGCTGAGGACATCATGCAGAATGTGGACTAATTGGTTTG 5 0.125 No Hit TGAGAATGTCAAGACTCCTGATGTGCATACTTTCTTGCAGCATTTGGTGA 5 0.125 No Hit GGTTGGTCTTCCTCCTGGTGTCCTCAATATTTTAACTGGGTTGGGCACTG 5 0.125 No Hit ACTCTACCTCACATAGCTCCTCTTTTTCTCTTCACTTCACCACCACCACC 5 0.125 No Hit CAGGAAGATACATGGGCAGTCATCTCTCATTTCAAAGCTTTCCTCAAACT 5 0.125 No Hit CAAAACTCATTATTGCTGGTGCTAGTGCTTATCCTCGAGATTTTGATTAT 5 0.125 No Hit CTAAGAGCGCCAAGCTCTCTGTGACCAAGAAACCAACCAAAGGAGGAGGA 5 0.125 No Hit GATTGCACCATTTGATATAGAATTTTCAGTCACATACAAGGAACTCGACT 5 0.125 No Hit AGAGAAATATTTCAACCTTGTGAAACCGAAGAAAACCTCTCCTCCTCTTC 5 0.125 No Hit CAAGGAGAGAAAAAAGAAACTCCTCAGGAGAGACTCAAAAGGATAATGAG 5 0.125 No Hit GCATAAATTTGGTGATGGGCCATTCTTCCTTGGTCAAGAATTCAGTTTGG 5 0.125 No Hit CCTTTTTGTATTGCAGGAAGAGAGGAGAGGATGGTAAAGGGGGTGATCTG 5 0.125 No Hit AACACCACCAATTCTTCCTCCACCAGAGCCTCCAGTAATACCAACACCAC 5 0.125 No Hit GATAAGGACAAGAATGGTGACGAGCAGGAAAAAGGTCTCTTTGGTCATGG 5 0.125 No Hit CGGGGGTCAGGACAAGATCCGCCCCTTGTGGAGACATTACTTCCAGAACA 5 0.125 No Hit ATCCCAGTTCTCGACCAAATCACTTTGCAAGGTGTGATTGGTTCAAACAT 5 0.125 No Hit GCATTTGACTATTCTTATTGAGAGATCAGCTCTTTCATTGAGCCTCTCGA 5 0.125 No Hit CCTAATTGCAGATGCTGGTGCTTTAGCACTCTCCGGGGACGCATCAACAT 5 0.125 No Hit GTTTTACGTTGTTTCTCCATTAGTTGCAAGAGAAACATCAAAAGAAGTAG 5 0.125 No Hit GTCCTTAGAAGATGTGAGCCAAGGAAAAGAACTCTTGGCTGTTTTGGAAC 5 0.125 No Hit TGCAGAGTGGAAGGGCTATAATTTGCCAGTCTTGTAAGACGTTCTTGGCA 5 0.125 No Hit TTCACCTCTCAGGTCATCATCATGAACCACCCTGGTCAAATTGGGAATGG 5 0.125 No Hit CTTCCAGCGATCTCGACGATCCTTTAATCAGGCAAGTCGTGTCAGAGGGC 5 0.125 No Hit TGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGGCAAGCTTAGAA 5 0.125 No Hit CTTCTGGAAAATGGAATGGAGACCCCAATGACAAAGGTATCCAAACAAGT 5 0.125 No Hit ATTGACCCTTCTTCAATCCATGGGATCAGTCTTTTGCCAGGTTTGTTTCA 5 0.125 No Hit AATAGTGATAGTACTAATGGGAATCGGTGTGGTGGATTTTTTCCAAGAGG 5 0.125 No Hit CCTCCATGAAGCTTGGCTGCAACATGGGCGTTTGAATTGGAGGACTCTAT 5 0.125 No Hit AGAGGACATGTCGCGATCATTCAAGGAGGAGATGCTGGGGTATTGGATTT 5 0.125 No Hit CGTTAGTGCCCGTGAGCTCCGGAGAGAGGGTCATGATGTTGTAGTCTTTG 5 0.125 No Hit CCGTGCAGCTGTGGGTGCTGGCATCGGACTTCCAGGTCCCCTGACTGAAA 5 0.125 No Hit GCGACGCACAAGGCGAGCGCAAGGAAGGGTTTGTTGACAAGATCAAGGGC 5 0.125 No Hit GCTCAATTGTGAGAGAAAAGAGCAAGAAACATTCAGGTGTGAGAAAGAGG 5 0.125 No Hit GAGTTGCATAACTCCGGTTCAATTTCAAGCAATCACTCTAGAAGTGGCAT 5 0.125 No Hit GGTAAGAGCGAGGGTTCTTGGTATTTTGTTAGAAGATTATGAGGGTTAGC 5 0.125 No Hit AGTAAATGGAGGTGCTTCAGAGGATGCTAAATCTGAGAAGAAGAAGAAAA 5 0.125 No Hit AGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTG 5 0.125 No Hit CAGCAAGGGCAGGACTGTAGACTTCAAAAACACCCTCCTGATAATGACTT 5 0.125 No Hit GAGAGAAGAAAAAAAAGAAGAAGAAAACCAAAACTGACCACCAGAGAGAG 5 0.125 No Hit CAAACGCTCAACTACTTCAAAGAGTGTCTCACCTTGCAATGAACCAGTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0375 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.16249999999999998 0.0 0.0 0.0 0.0 76-77 0.21250000000000002 0.0 0.0 0.0 0.0 78-79 0.325 0.0 0.0 0.0 0.0 80-81 0.4 0.0 0.0 0.0 0.0 82-83 0.4625 0.0 0.0 0.0 0.0 84-85 0.5 0.0 0.0 0.0 0.0 86-87 0.6499999999999999 0.0 0.0 0.0 0.0 88-89 0.7749999999999999 0.0 0.0 0.0 0.0 90-91 0.825 0.0 0.0 0.0 0.0125 92-93 0.975 0.0 0.0 0.0 0.025 94-95 1.1875 0.0 0.0 0.0 0.025 96-97 1.225 0.0 0.0 0.0 0.025 98-99 1.5125000000000002 0.0 0.0 0.0 0.025 100-101 1.7125 0.0 0.0 0.0 0.025 102-103 1.775 0.0 0.0 0.0 0.025 104-105 1.9125 0.0 0.0 0.0 0.025 106-107 2.2 0.0 0.0 0.0 0.025 108-109 2.3625 0.0 0.0 0.0 0.025 110-111 2.7375 0.0 0.0 0.0 0.025 112-113 2.9625 0.0 0.0 0.0 0.025 114-115 3.2874999999999996 0.0 0.0 0.0 0.025 116-117 3.875 0.0 0.0 0.0 0.025 118-119 4.325 0.0 0.0 0.0 0.025 120-121 4.637499999999999 0.0 0.0 0.0 0.025 122-123 5.075 0.0 0.0 0.0 0.025 124-125 5.4 0.0 0.0 0.0 0.025 126-127 5.949999999999999 0.0 0.0 0.0 0.025 128-129 6.574999999999999 0.0 0.0 0.0 0.025 130-131 7.2625 0.0 0.0 0.0 0.025 132-133 8.025 0.0 0.0 0.0 0.025 134-135 8.375 0.0 0.0 0.0 0.025 136-137 8.7625 0.0 0.0 0.0 0.025 138-139 9.3 0.0 0.0 0.0 0.025 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTCTC 10 0.006830828 145.0 1 >>END_MODULE Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409126 spots for SRR26075404.sra Written 1409126 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra Read 1409107 spots for SRR26075404.sra Written 1409107 spots for SRR26075404.sra SRR ids: ['SRR26075404.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_s8n1v6jt SRR26075404.sra spots: 28182159 blocks: [[1, 1409107], [1409108, 2818214], [2818215, 4227321], [4227322, 5636428], [5636429, 7045535], [7045536, 8454642], [8454643, 9863749], [9863750, 11272856], [11272857, 12681963], [12681964, 14091070], [14091071, 15500177], [15500178, 16909284], [16909285, 18318391], [18318392, 19727498], [19727499, 21136605], [21136606, 22545712], [22545713, 23954819], [23954820, 25363926], [25363927, 26773033], [26773034, 28182159]] SRR26075404 file size 10405134 SRR26075404 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075404 SRR26075404_1.fastq SRR26075404_2.fastq Input file: SRR26075404_1.fastq Paired file: SRR26075404_2.fastq trimmed: SRR26075404-trimmed-pair1.fastq, SRR26075404-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:35:23 2025 >> started Wed Feb 12 01:36:00 2025 >> done (37.047s) 28182159 read pairs processed; of these: 124 ( 0.00%) short read pairs filtered out after trimming by size control 68928 ( 0.24%) empty read pairs filtered out after trimming by size control 28113107 (99.75%) read pairs available; of these: 4194156 (14.92%) trimmed read pairs available after processing 23918951 (85.08%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 13 0.00% 19 14 0.00% 20 8 0.00% 21 12 0.00% 22 14 0.00% 23 11 0.00% 24 8 0.00% 25 15 0.00% 26 17 0.00% 27 20 0.00% 28 30 0.00% 29 13 0.00% 30 16 0.00% 31 26 0.00% 32 22 0.00% 33 23 0.00% 34 40 0.00% 35 32 0.00% 36 27 0.00% 37 30 0.00% 38 27 0.00% 39 25 0.00% 40 44 0.00% 41 45 0.00% 42 47 0.00% 43 60 0.00% 44 47 0.00% 45 64 0.00% 46 63 0.00% 47 77 0.00% 48 111 0.00% 49 96 0.00% 50 111 0.00% 51 116 0.00% 52 158 0.00% 53 154 0.00% 54 148 0.00% 55 199 0.00% 56 201 0.00% 57 220 0.00% 58 280 0.00% 59 342 0.00% 60 388 0.00% 61 406 0.00% 62 476 0.00% 63 566 0.00% 64 696 0.00% 65 668 0.00% 66 845 0.00% 67 876 0.00% 68 966 0.00% 69 1094 0.00% 70 1330 0.00% 71 1433 0.01% 72 1796 0.01% 73 2076 0.01% 74 2388 0.01% 75 2640 0.01% 76 2975 0.01% 77 3307 0.01% 78 3610 0.01% 79 4215 0.01% 80 4550 0.02% 81 5553 0.02% 82 5913 0.02% 83 6801 0.02% 84 7703 0.03% 85 8876 0.03% 86 9701 0.03% 87 10827 0.04% 88 11351 0.04% 89 12369 0.04% 90 13338 0.05% 91 14923 0.05% 92 15799 0.06% 93 17552 0.06% 94 19563 0.07% 95 20487 0.07% 96 22842 0.08% 97 24530 0.09% 98 25668 0.09% 99 27099 0.10% 100 28556 0.10% 101 29622 0.11% 102 30831 0.11% 103 33654 0.12% 104 35476 0.13% 105 38355 0.14% 106 40117 0.14% 107 41893 0.15% 108 43189 0.15% 109 45032 0.16% 110 45670 0.16% 111 48421 0.17% 112 49216 0.18% 113 51852 0.18% 114 53561 0.19% 115 56966 0.20% 116 58055 0.21% 117 61686 0.22% 118 63563 0.23% 119 65526 0.23% 120 65595 0.23% 121 68210 0.24% 122 69212 0.25% 123 71110 0.25% 124 74892 0.27% 125 75925 0.27% 126 79347 0.28% 127 80802 0.29% 128 84021 0.30% 129 84900 0.30% 130 87698 0.31% 131 88496 0.31% 132 89002 0.32% 133 92544 0.33% 134 92572 0.33% 135 95150 0.34% 136 97025 0.35% 137 98820 0.35% 138 102044 0.36% 139 103624 0.37% 140 104659 0.37% 141 107870 0.38% 142 107751 0.38% 143 108856 0.39% 144 112465 0.40% 145 114906 0.41% 146 115070 0.41% 147 117229 0.42% 148 119000 0.42% 149 120455 0.43% 150 122443 0.44% 151 23918951 85.08% 28113107 reads passed initial QC criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=5.14 fanout-score-rank=24 prefix-density=0.41 prefix-fanout=3.7 sequence=ACCATCACCAATA criterion=fanout-score sequence-density=0.03 sequence-density-rank=34 fanout-score=134.00 fanout-score-rank=1 prefix-density=0.34 prefix-fanout=11.8 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=2.89 fanout-score-rank=25 prefix-density=0.33 prefix-fanout=2.9 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=179.10 fanout-score-rank=1 prefix-density=0.21 prefix-fanout=8.1 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGGCAAGCTTAGAAAGATCAGCACCAGACCACTTAACATCAAAGATATTGACGTCGGAGAGGGGAGCAGTCCTGTTAATAAGTTTCAGAGGTCCATGACTATGCCAGGAACTCCAGGGACACCGACGACACCAGTGACCCCTACAACCCCAGTGTCGGCGCGTAGCAATGTTTGGAGGAGCGTGTTCCACCCTGGTAGCAACCTTGCTACTAAGAATATTGGTGCTCATGTTTTTGACAAGCCACAGCCTAACACACCCACTGTCTATGACTGGATGTACAGTGGAGAGACGAAGAGCGAGCATCGTTGATGAGGTTGCCTTCAACCAAGGTTGCCCATGTAAATACGTACTGTGTTTTGTTTTTCAGTACTCATCTGCAATATGTCTCTTGTTGTTATGGTTCTACGGTTCTACCGTGCCTGGAA SRR26075404 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:36:43 Started mapping on | Feb 12 01:36:44 Finished on | Feb 12 01:40:58 Mapping speed, Million of reads per hour | 398.45 Number of input reads | 28113107 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 25885146 Uniquely mapped reads % | 92.08% Average mapped length | 293.46 Number of splices: Total | 24475770 Number of splices: Annotated (sjdb) | 23866898 Number of splices: GT/AG | 24027106 Number of splices: GC/AG | 351575 Number of splices: AT/AC | 23020 Number of splices: Non-canonical | 74069 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 3.13 Insertion rate per base | 0.02% Insertion average length | 2.57 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 876420 % of reads mapped to multiple loci | 3.12% Number of reads mapped to too many loci | 167689 % of reads mapped to too many loci | 0.60% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.92% % of reads unmapped: other | 0.29% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1351541 1351541 1351541 N_multimapping 876420 876420 876420 N_noFeature 673183 25577903 821285 N_ambiguous 301400 1634 141180 UnstrandedReadsAssigned:24910563 PositiveStrandReadsAssigned:305609 NegativeStrandReadsAssigned:24922681 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075404 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075404-trimmed-pair1.fastq SRR26075404-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 28,113,107 reads, 25,395,308 reads pseudoaligned [quant] estimated average fragment length: 216.239 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,190 rounds 52401 SRR26075404.ke.tsv 34699 SRR26075404.se.tsv 87100 total ==> SRR26075404.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1802.76 2764 52.9102 Potri.005G024800.1.v4.1 1035 819.761 1428 60.1147 Potri.004G059700.1.v4.1 961 745.761 48 2.22117 Potri.007G009000.2.v4.1 1416 1200.76 0 0 Potri.003G141000.2.v4.1 2943 2727.76 1047 13.2459 Potri.016G087400.1.v4.1 270 87.9916 1808 709.083 Potri.015G069301.1.v4.1 564 350.867 0 0 Potri.010G195200.1.v4.1 1773 1557.76 411 9.10502 Potri.012G127500.1.v4.1 977 761.761 8874 402.013 ==> SRR26075404.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 568 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 354 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 890 SRR26075404 completed mapping pipeline successfully