Starting /dee2/code/volunteer_pipeline.sh SRR26075405 current disk space = 3050139508736 free memory = 1573544052 SRR26075405 SRAfilesize 0ed3d00a84b3577e43499aa383c5a3e7 SRR26075405.sra SRR26075405.sra file validated SRR26075405 is paired end SRR26075405 is conventional basespace SRR26075405 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075405_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.64725 37.0 37.0 37.0 37.0 37.0 2 36.5835 37.0 37.0 37.0 37.0 37.0 3 36.655 37.0 37.0 37.0 37.0 37.0 4 36.6825 37.0 37.0 37.0 37.0 37.0 5 36.6635 37.0 37.0 37.0 37.0 37.0 6 36.686 37.0 37.0 37.0 37.0 37.0 7 36.5755 37.0 37.0 37.0 37.0 37.0 8 36.673 37.0 37.0 37.0 37.0 37.0 9 36.677 37.0 37.0 37.0 37.0 37.0 10-14 36.6382 37.0 37.0 37.0 37.0 37.0 15-19 36.6314 37.0 37.0 37.0 37.0 37.0 20-24 36.4988 37.0 37.0 37.0 37.0 37.0 25-29 36.4875 37.0 37.0 37.0 37.0 37.0 30-34 36.447199999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.366600000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.3243 37.0 37.0 37.0 37.0 37.0 45-49 36.24929999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.260000000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.1543 37.0 37.0 37.0 37.0 37.0 60-64 36.161199999999994 37.0 37.0 37.0 37.0 37.0 65-69 36.108 37.0 37.0 37.0 37.0 37.0 70-74 36.1267 37.0 37.0 37.0 37.0 37.0 75-79 36.017 37.0 37.0 37.0 37.0 37.0 80-84 36.006299999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.89319999999999 37.0 37.0 37.0 37.0 37.0 90-94 35.899 37.0 37.0 37.0 37.0 37.0 95-99 35.8483 37.0 37.0 37.0 37.0 37.0 100-104 35.7855 37.0 37.0 37.0 37.0 37.0 105-109 35.8155 37.0 37.0 37.0 37.0 37.0 110-114 35.593599999999995 37.0 37.0 37.0 37.0 37.0 115-119 35.593500000000006 37.0 37.0 37.0 37.0 37.0 120-124 35.622400000000006 37.0 37.0 37.0 37.0 37.0 125-129 35.4226 37.0 37.0 37.0 37.0 37.0 130-134 35.316500000000005 37.0 37.0 37.0 34.6 37.0 135-139 35.208800000000004 37.0 37.0 37.0 29.8 37.0 140-144 35.1444 37.0 37.0 37.0 27.4 37.0 145-149 35.1332 37.0 37.0 37.0 25.0 37.0 150-151 34.9475 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 3.0 23 5.0 24 8.0 25 4.0 26 12.0 27 9.0 28 17.0 29 23.0 30 34.0 31 42.0 32 61.0 33 85.0 34 165.0 35 521.0 36 2794.0 37 216.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.82912184138104 15.086314736052039 8.656492369276958 37.42807105328997 2 17.8 13.750000000000002 34.075 34.375 3 17.224999999999998 16.925 27.900000000000002 37.95 4 21.95 23.45 23.825 30.775000000000002 5 23.0 31.35 23.1 22.55 6 23.674999999999997 32.125 21.875 22.325 7 15.8 28.249999999999996 39.625 16.325 8 19.05 26.55 30.0 24.4 9 18.475 25.1 33.5 22.925 10-14 19.975 29.544999999999998 28.144999999999996 22.335 15-19 20.225 26.86 28.715000000000003 24.2 20-24 20.285 28.555000000000003 27.82 23.34 25-29 20.044999999999998 27.650000000000002 27.205000000000002 25.1 30-34 20.01 28.305000000000003 27.73 23.955000000000002 35-39 19.62 28.025 28.025 24.33 40-44 20.25 28.535 27.860000000000003 23.355 45-49 21.525 27.305 26.71 24.46 50-54 20.66 28.134999999999998 27.32 23.885 55-59 19.384999999999998 27.389999999999997 27.560000000000002 25.665 60-64 20.54 27.589999999999996 27.215 24.654999999999998 65-69 20.53 27.36 27.66 24.45 70-74 20.27 27.200000000000003 27.87 24.66 75-79 21.015 27.045 28.09 23.849999999999998 80-84 21.68 27.284999999999997 26.38 24.654999999999998 85-89 20.979999999999997 26.35 28.365000000000002 24.305 90-94 20.810000000000002 28.18 26.6 24.41 95-99 21.015 26.63 28.13 24.224999999999998 100-104 21.415 26.935 28.025 23.625 105-109 22.34 27.544999999999998 27.405 22.71 110-114 21.465 28.07 26.650000000000002 23.815 115-119 22.264999999999997 27.665 26.255 23.815 120-124 22.005 27.200000000000003 26.52 24.275 125-129 21.81 27.095000000000002 27.235 23.86 130-134 21.275 27.37 26.884999999999998 24.47 135-139 21.375 26.950000000000003 26.545 25.130000000000003 140-144 20.765 27.91 27.67 23.655 145-149 22.735 26.32 26.790000000000003 24.154999999999998 150-151 20.575 28.7375 25.2 25.4875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.5 18 1.0 19 1.5 20 3.5 21 2.5 22 0.0 23 0.0 24 0.0 25 1.0 26 1.5 27 4.5 28 7.5 29 6.5 30 6.5 31 13.0 32 21.5 33 21.5 34 35.5 35 63.5 36 75.0 37 89.5 38 105.5 39 127.5 40 161.5 41 198.0 42 256.0 43 272.0 44 259.5 45 274.0 46 289.0 47 277.5 48 227.5 49 213.0 50 196.0 51 147.5 52 129.5 53 103.5 54 80.0 55 66.5 56 53.0 57 46.0 58 41.0 59 30.5 60 15.5 61 13.0 62 7.0 63 8.0 64 13.0 65 6.0 66 5.5 67 6.0 68 3.5 69 3.0 70 1.0 71 1.5 72 2.5 73 1.0 74 0.0 75 0.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.37499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 58.06896551724138 31.574999999999996 2 21.74712643678161 23.65 3 9.655172413793103 15.75 4 5.241379310344827 11.4 5 2.436781609195402 6.625 6 1.1494252873563218 3.75 7 0.8735632183908045 3.325 8 0.5517241379310345 2.4 9 0.13793103448275862 0.675 >10 0.13793103448275862 0.8500000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTGCCTGGGATCCATGCTGATCACATACAACCAATCTAGGAACAGGGAA 14 0.35000000000000003 No Hit CTTCCTTCAATAACATTAGGCTCCACAGCATCTTCTTCTGAACTCAGTAT 10 0.25 No Hit AGACAGAGGCCGTGTCAGTCTTCCCAAATGGAGAAACAAGCTGGTGAAGA 10 0.25 No Hit CCAAAATCATTCAATCTTGCACAGAAGGCCATATTCCTACTTCTGAATAC 9 0.22499999999999998 No Hit AGTCTGTCAACTTCTCCCCAAGATTTGTCATCACATGTCGTAGCTCTGCA 9 0.22499999999999998 No Hit GTTGCCAATGACTTCAGCTGACTTGGCGACAGTTCATCATTAAAGATGAG 9 0.22499999999999998 No Hit GTGAATTACTGGGCCTCAATTGTGTTTAAATTGCTAGCACCATCAGCTGT 8 0.2 No Hit GCAACCTTGGTTTTTGTACCGAGCTCAATAAATTCCTGGACAACCCTACC 8 0.2 No Hit CCGGTGGTCTTACTATCATGATACTCCGTGTCACTCTCTTCTCATCAACC 8 0.2 No Hit ACCGCAATAGTTTCTATCCTGAGCTCCCTTAGCATTTGCATTATGTCTAA 8 0.2 No Hit CCCATAAATTATGGTAGCCTCGATCTCCAGTGTGAAAATAGGACTCTTGA 8 0.2 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 8 0.2 No Hit TCCCCATTCAAGAGCTTGTGGTTCTCTTCTTTAGGCTTGCTATCCTTGTC 8 0.2 No Hit GCTCTCTTTCTACGTTCTGCTTCTGCCTGCATCTCCATAGCTTGCTTCAC 8 0.2 No Hit CTCACAAGCAAGTCGTGGCGTAGGAATTGAATTAATGAACTTGGTGGCAG 8 0.2 No Hit GTCCAATATGTATGCTTATTATCTCCCTCATAATTGATTTCTTCCTCTCT 8 0.2 No Hit GCCGAGTATCTGGAGACAGCTTGAGAATGAGATGCCTTCAGCTTTCTCTT 8 0.2 No Hit GTCCAACCAAATTTGTCAAAATTGTGATAATAATAGCTGCCCTAAAATTG 8 0.2 No Hit GCAACATGAACTGGAACATCAATTGTTGTTGTAGTTGTGTCTTGTTGGGT 7 0.17500000000000002 No Hit GACAGGACCTCGACCTCCTCCACACCTGCAAAGACAGAAGGATACGTTAG 7 0.17500000000000002 No Hit CTCTCCTGCTAAAGCCACTACCTCTACGTCTGAGAGAGTTTTCTTGTCTC 7 0.17500000000000002 No Hit AGCAAAATGGAAAACAAACTTCTTCAATTAGGGATGACCTTCTGGGATCT 7 0.17500000000000002 No Hit GAGCAGAGCTAGAAGACTTGCTATGAATTCCCTGTTTGCTTTCTTAGACA 7 0.17500000000000002 No Hit CTAGCATCCGCAACAGCCTCGGTGAAATTGTTGAGTTTGATATTGGCTTG 7 0.17500000000000002 No Hit CTCAACTACAGCCATTTTCTTCTTCTTTATTTTTGTCGTCTTAAATGACT 7 0.17500000000000002 No Hit CTGCTCTCAATGAGCCTCCTCAAGCTCCGGTTGTTTTCTGATAGCCTCTC 7 0.17500000000000002 No Hit GTCAGGCGATAATCCTCTCCTCTGCTACACTCCCTCTCACAAGCTAATTC 7 0.17500000000000002 No Hit AGATTTGTTGTTGTGCTTCTGTTTTCCATTTGGCGTGGCTCTGGGAGTTT 7 0.17500000000000002 No Hit CCATGTTAGCAGCAGCACCCCGAACCACAAGGCGAAAATCCCTCTTTAGC 7 0.17500000000000002 No Hit CCAAAACAGAAAAGGAGAATAAATCAGAACATGAATAAGAAGAAGCCTTG 7 0.17500000000000002 No Hit ACTGATAATAGTAGCAGACATTGCCAATTACCCAGAAGCAGCAGATCAAA 7 0.17500000000000002 No Hit CCCCATGTAAAGCCCGTGCTCCAGGCAATAATCCAGCAAGTTCATTTGCG 7 0.17500000000000002 No Hit AGGACGGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCA 7 0.17500000000000002 No Hit CCCCTCTTAAAAAAACAAACGACCCAGGTCCAGTTGGAAAGGGGGGCAAA 7 0.17500000000000002 No Hit CCTGAGACATTTATCGTTATTGAACATCCAAAATTAGGGCAATGAAACCT 7 0.17500000000000002 No Hit CAGGATTTGGTGATGGGAAGCCAGAAAACTTCCTTGGGCGCTTCACTTGG 7 0.17500000000000002 No Hit AACCCATCATTCGGCGGCGAAAGCGCAGAGGAAATCATAGGCAACAGAAG 7 0.17500000000000002 No Hit GCTGTTCATAGACTTCTACCAACAACGGAGTGAAATTACGGCACGGCCCG 6 0.15 No Hit CCTCTCTTGACCGGCAGTGTCCCAAATCTGAGCCTTAACTTCTTTTCCAT 6 0.15 No Hit TGGCCCTCAACCATCTTTCCAGGTAAAAGAAGCTGCAGTGCAGCCTCAAA 6 0.15 No Hit TCTAATTATATCTCTGCTAACGGGCTCTAAGTAATGGATCTTCATTCTAG 6 0.15 No Hit CCTGCTCGCTGGAAGAGAAGGCTTCCCGGATCCAAGCAAACGGGTTGCGG 6 0.15 No Hit CATCCAAGAGCTCAGTCACACCAGATTAGTTTCTCTGATCCTTAAATGGG 6 0.15 No Hit GTCGAGATAGAGAGGACGAGATGAGATTTTCACGCCTTTTATTGTCATGG 6 0.15 No Hit GTTTGGCGAAATGATATACGCAAGGGTTTTTGGTAACTCGATAACCAACA 6 0.15 No Hit CCGATCCGAATTTCTTCCAGTTCCAAACTCCTGTGAAGGCAACAGCCTGC 6 0.15 No Hit GCTGGAGTCATGTCGAGTGGATATAAAACCACATCATATACCTTCACTGA 6 0.15 No Hit TTTTGATCGATTATAAGCAAAAAAATCCTCACATAAATCATCATGGCCCT 6 0.15 No Hit ATTCAAAGTCTCAAAACTTGAAAACACCATGTTGGGGTTCAATCATCCAA 6 0.15 No Hit CTGCACGGTTCTCCATCTGACTCCATTCGGTTCGGCCTTTCTGGTACAAA 6 0.15 No Hit GTGCAAACTCTTCTTCCCGTGGTACCTCAAAAAGAGCAGTTGAAGGAGCA 6 0.15 No Hit GCCAGCTTGAGAATCTCGGTGAAGAAACAGCTTCAACCTTCTTCTCTGTA 6 0.15 No Hit CATCGCCTTCGGAAATCTCATCGATTCTTTTCTCATCTTCAAGTACACTG 6 0.15 No Hit GTCCAAACCTTGGTGGAAGATCTGCTCGCCAAGCCCTCCGCATCCCTCCT 6 0.15 No Hit CGTAACCGCACTGAGACCGAAATCCGTGATTTTCAAGTTAAAATTCTCAT 6 0.15 No Hit CCAATTTACACTGCTTCAGTTGTATAAACTAATTGAGCTGATCTACGTAT 6 0.15 No Hit CTCCTCTGCCTTCTTCTCCTCTTCTGCCTTCTTCTCCGCTTCGACAGTCG 6 0.15 No Hit ACGGCTGTGAAACCACACTTTCATTTGGCGAGAAACTAGCTGTGGATGGA 6 0.15 No Hit AGCATGATTCTCCCACGATCTTGACTCCTTTCTTGAACAAAACCGTGAGC 6 0.15 No Hit GATCTGAAGACTGGCCTGCTGACAAGCTTTTGGGCCCAAAAAAGCTAATG 6 0.15 No Hit GATCTGTATAGTCAATTCCATCAGCTTCAGAGAGCGCAGATGCCATACTC 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGCCTTATCTCGTAT 6 0.15 TruSeq Adapter, Index 7 (97% over 35bp) GTGCAAAGCTTGGGATCCTTAACCTCAGCAAAATGAAAAGGATCAAGGCG 5 0.125 No Hit GGCAAATCTTGTCCCTGGCTTCAGCCTTATTCAGATACAGAACTATAAGA 5 0.125 No Hit ATGGCTTCCAAAGATAACAGCGGTACCTTGCCCTTTTTGGCACAATAACC 5 0.125 No Hit CCATTGTTTCGAGTTGTAACATGCAAGGTAATGTAAAGAAGCATGAAAAC 5 0.125 No Hit GCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGA 5 0.125 No Hit CCCTAAACTATTCAAGCTATCATGGTGTAATACATACAGCCTTGAAACCC 5 0.125 No Hit GCTATGAAATGTTAATAATAAACAAATTTTTTTAAAAAAAAAAAAAACGT 5 0.125 No Hit CCGTCTTTCAGCATTTTCCTTGATTATGTGTGTGATTAAGTCTTTCTCTT 5 0.125 No Hit GCAAGATCCATTCCATGAGCTTCACCTGCCCAGAAAATGTTTCTTCTTTC 5 0.125 No Hit CCCAAATTTACACACATTTCCTTCTTTGAGCTCCGTTAATTTAAAGATAA 5 0.125 No Hit GGTTTCTGAAAAATAGAAGAGAAGGAAAGAGATTGAAGATTTCTTCTCTT 5 0.125 No Hit CTCCAACCTTGGAAAAGATACGGCGAGCAAGGCCATTCTTTTGCTCCAAG 5 0.125 No Hit GCAGGATCCGGTTTGGTAGCTGCTTCATTTGAATCAGTAGCCAAATCAGG 5 0.125 No Hit AGTCGAGCAGCCATCTTCTGGTCCTCCTCTATGCTATTGTTTCCAAACCA 5 0.125 No Hit GCCTCGATATCTTGTTTGCTTTCATGGGAGAAGGAAGTCGCAGAATCTGG 5 0.125 No Hit GTCAGGACAATTCACAGTGATAACAGTAGGGTCTCCTTCTTTCTCTGATT 5 0.125 No Hit TTCGCATCTCCGAAGGCTTAGTGACCCTTAACTGATTACCAAGTTGAACT 5 0.125 No Hit GATTTGGAAGTTTTAGGTTCTCCGGGTTGACTAGAACCCAATCCTGATGA 5 0.125 No Hit TCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCA 5 0.125 No Hit GCCACCTGCGGAAAAGGATAAATATATAACAGAGTGGGCAGCACATAAGT 5 0.125 No Hit GTGCAGATCATTGAAAGATGATCAACAGGCAATCTTTCACCTGCTGCATC 5 0.125 No Hit TAAAAGAAAACTTTTCTGTGTTAACCTAACCAAATGTAGAGACAACTGAA 5 0.125 No Hit CCCCACTCTTCTTTGGTTGAACTTTTGAAGCAGCTGGAGTTGCAGATTTG 5 0.125 No Hit CAGTATTGATCTTAGTTTCAGAGTCTCGAAGAGAAGCAGAGTCAATGCTT 5 0.125 No Hit CTCCATGATTTGCACAAACGAATCTTTCCATCTTTATGTGATGCTAGCTG 5 0.125 No Hit CTATCTCCTTCCTGTATTTCCCACTGTTTCTTCAGCTCATGGATAGTTTT 5 0.125 No Hit GGATCATTCTAGACTGATATAGTTTAGCGATTGTGATGGAGGGAAGATTC 5 0.125 No Hit CACCAAGCGAAGTAACTCCCAAAACCACCGAGTTAAGCATCAACCCCAAT 5 0.125 No Hit ACCACCCAATAGCAAACAGGAGCAGCCCAGAAAATGAATCAGAGGTCCGG 5 0.125 No Hit GTGCAAACCATCCTCTTGCTGTTCAACTCTCACTTCATCAATATCCAATG 5 0.125 No Hit CTTCTCGCGTACAATAGGCATAAAATGTCCTTGCACTTGGGTAGCAGTTG 5 0.125 No Hit CACCAGGTGATTGTTTGTTTATTACTGTCACCTTCCACATGGGCTTGGTA 5 0.125 No Hit CCGGTTTACATAGTCATCACAGCTGCCATTGGAAAGCCTCTGAATTGGGA 5 0.125 No Hit CCCCTCTTCTCTTCCGGTTCAGATGGCGTATTTGTGTGAGAAAATGCGGC 5 0.125 No Hit CTGTAAAGGATGCTCTGGGCTAGTATATCTCATCATCAGGGACATCAGGA 5 0.125 No Hit GGAAGCTATCATCAAAATTCTCAGTGGCCACAACTAAGTCCTGGAAAGTG 5 0.125 No Hit ACCCTCTTAGGCCTGCAGAATTAACAGACAAGTTTAGGAGCGCACCTCGC 5 0.125 No Hit GCAGAATATCACAAGTTACTAATGCTGAATTTAGATGACAAAGTATACCG 5 0.125 No Hit GTTGTCATCATCTGTCTGATTGGAAGAGTGTTCAGAGGCACTCTTACCTT 5 0.125 No Hit GTCCAGTCAAACTCTGTAGCTTATTCTTAAGAGCATTAACAAGGCCAGCT 5 0.125 No Hit CCCACTAGGAAACTCCTTTTCACCACATCCATTCATTCTAAGATACCTCA 5 0.125 No Hit GCTTTGTTTTGGTGGATGAAAGAGAGGGAGTGAGAGCAGTGGTAACGGTT 5 0.125 No Hit TGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGA 5 0.125 No Hit CTGGGATTTACGATAGCTTCCACCTCTGAACTCCATTGGCATCACTTGCA 5 0.125 No Hit GCCCGTTCGACTCGAAGGATTAGATTGTCATAACCATATCCATTGAGCTT 5 0.125 No Hit TATGCCTGAACCTGGGAAGAAGTTTATGTGGTCTAGGGTTTGCCAGACTG 5 0.125 No Hit GCTAAACCATAGGCATTCTCATGGATGGCCAGCTCAGATGGCTCATTGGC 5 0.125 No Hit GTCCTTGCTTGGAATTGACCCTTGATCTGTTCTACTATGGAGAAAAAATG 5 0.125 No Hit GCACGCACCACGCCTGCTGAACTTGTGCAGCAGCTTCCACATAAAACAAT 5 0.125 No Hit GGGTGGGCGGCCTGATATGCCCATACAGCCAACCCCAGACACAAGACCAC 5 0.125 No Hit CTGTAATTGGGGAGACGCTGGAAATGATGACATGTTTCCTGTAGAAGAGC 5 0.125 No Hit GCTGGAACTTTCTCCTTGACTTTGTCAAGGAAACCCTTCTTCTCCTCTGG 5 0.125 No Hit ACGAGAAATAAAGAGCATAAACTGTATAAAGAGCACACCAAAAGCCCAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.0625 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.2 0.0 0.0 0.0 0.0 78-79 0.225 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.2875 0.0 0.0 0.0 0.0 84-85 0.4 0.0 0.0 0.0 0.0 86-87 0.45 0.0 0.0 0.0 0.0 88-89 0.6000000000000001 0.0 0.0 0.0 0.0 90-91 0.775 0.0 0.0 0.0 0.0 92-93 1.0499999999999998 0.0 0.0 0.0 0.0 94-95 1.1 0.0 0.0 0.0 0.0 96-97 1.2 0.0 0.0 0.0 0.0 98-99 1.45 0.0 0.0 0.0 0.0 100-101 1.6875 0.0 0.0 0.0 0.0 102-103 2.1125 0.0 0.0 0.0 0.0 104-105 2.2249999999999996 0.0 0.0 0.0 0.0 106-107 2.425 0.0 0.0 0.0 0.0 108-109 2.7 0.0 0.0 0.0 0.0 110-111 3.0875 0.0 0.0 0.0 0.0 112-113 3.4375 0.0 0.0 0.0 0.0 114-115 3.8125 0.0 0.0 0.0 0.0 116-117 4.225 0.0 0.0 0.0 0.0 118-119 4.725 0.0 0.0 0.0 0.0 120-121 5.5875 0.0 0.0 0.0 0.0 122-123 5.875 0.0 0.0 0.0 0.0 124-125 6.275 0.0 0.0 0.0 0.0 126-127 6.7 0.0 0.0 0.0 0.0 128-129 7.1125 0.0 0.0 0.0 0.0 130-131 7.3875 0.0 0.0 0.0 0.0 132-133 7.9375 0.0 0.0 0.0 0.0 134-135 8.625 0.0 0.0 0.0 0.0 136-137 9.575 0.0 0.0 0.0 0.0 138-139 10.2875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTCGA 10 0.006830828 145.0 4 TCGACTC 10 0.006830828 145.0 7 CGCCTTC 10 0.006830828 145.0 4 TTCGACT 10 0.006830828 145.0 6 CCGTTCG 10 0.006830828 145.0 3 CCCGTTC 10 0.006830828 145.0 2 TCGCCTT 10 0.006830828 145.0 3 GACTCGA 10 0.006830828 145.0 9 CGACTCG 10 0.006830828 145.0 8 CGACTCA 10 0.006830828 145.0 145 GTTCGAC 10 0.006830828 145.0 5 >>END_MODULE SRR26075405 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075405_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.362 37.0 37.0 37.0 37.0 37.0 2 36.311 37.0 37.0 37.0 37.0 37.0 3 36.3755 37.0 37.0 37.0 37.0 37.0 4 36.3175 37.0 37.0 37.0 37.0 37.0 5 36.433 37.0 37.0 37.0 37.0 37.0 6 36.2755 37.0 37.0 37.0 37.0 37.0 7 36.127 37.0 37.0 37.0 37.0 37.0 8 36.26 37.0 37.0 37.0 37.0 37.0 9 36.323 37.0 37.0 37.0 37.0 37.0 10-14 36.2755 37.0 37.0 37.0 37.0 37.0 15-19 36.118399999999994 37.0 37.0 37.0 37.0 37.0 20-24 36.011900000000004 37.0 37.0 37.0 37.0 37.0 25-29 35.9071 37.0 37.0 37.0 37.0 37.0 30-34 35.7547 37.0 37.0 37.0 37.0 37.0 35-39 35.706900000000005 37.0 37.0 37.0 37.0 37.0 40-44 35.6812 37.0 37.0 37.0 37.0 37.0 45-49 35.611 37.0 37.0 37.0 37.0 37.0 50-54 35.4578 37.0 37.0 37.0 37.0 37.0 55-59 35.5583 37.0 37.0 37.0 37.0 37.0 60-64 35.5918 37.0 37.0 37.0 37.0 37.0 65-69 35.4901 37.0 37.0 37.0 37.0 37.0 70-74 35.398399999999995 37.0 37.0 37.0 37.0 37.0 75-79 35.348600000000005 37.0 37.0 37.0 37.0 37.0 80-84 35.3657 37.0 37.0 37.0 37.0 37.0 85-89 35.3277 37.0 37.0 37.0 37.0 37.0 90-94 35.2075 37.0 37.0 37.0 37.0 37.0 95-99 35.2962 37.0 37.0 37.0 37.0 37.0 100-104 35.275299999999994 37.0 37.0 37.0 37.0 37.0 105-109 35.164199999999994 37.0 37.0 37.0 29.8 37.0 110-114 35.1202 37.0 37.0 37.0 34.6 37.0 115-119 35.2119 37.0 37.0 37.0 34.6 37.0 120-124 35.012 37.0 37.0 37.0 25.0 37.0 125-129 34.9766 37.0 37.0 37.0 25.0 37.0 130-134 35.029999999999994 37.0 37.0 37.0 29.8 37.0 135-139 34.8521 37.0 37.0 37.0 25.0 37.0 140-144 34.8755 37.0 37.0 37.0 25.0 37.0 145-149 34.747299999999996 37.0 37.0 37.0 25.0 37.0 150-151 34.4245 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 8.0 14 14.0 15 8.0 16 11.0 17 7.0 18 8.0 19 6.0 20 6.0 21 12.0 22 13.0 23 13.0 24 14.0 25 11.0 26 15.0 27 18.0 28 18.0 29 27.0 30 24.0 31 41.0 32 38.0 33 85.0 34 173.0 35 636.0 36 2544.0 37 249.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.949999999999996 21.825 10.9 25.324999999999996 2 29.225 26.400000000000002 27.500000000000004 16.875 3 23.325000000000003 27.85 29.925 18.9 4 26.674999999999997 32.550000000000004 22.175 18.6 5 28.525 34.275 21.65 15.55 6 24.099999999999998 39.0 20.625 16.275000000000002 7 23.075000000000003 23.0 35.099999999999994 18.825 8 22.525000000000002 25.124999999999996 24.625 27.725 9 22.475 25.75 26.75 25.025 10-14 24.68 28.444999999999997 25.585 21.29 15-19 25.585 27.97 26.669999999999998 19.775000000000002 20-24 25.124999999999996 27.810000000000002 26.515 20.549999999999997 25-29 25.11 27.665 26.39 20.835 30-34 24.565 28.095 26.35 20.990000000000002 35-39 25.285000000000004 26.810000000000002 26.119999999999997 21.785 40-44 24.86 28.49 25.7 20.95 45-49 24.310000000000002 28.07 26.77 20.849999999999998 50-54 22.805 28.175 27.565 21.455 55-59 24.545 27.935 26.875 20.645 60-64 25.145 28.26 25.865 20.73 65-69 25.515 29.035 25.2 20.25 70-74 23.765 28.549999999999997 26.695 20.990000000000002 75-79 23.919999999999998 29.310000000000002 25.740000000000002 21.029999999999998 80-84 24.555 28.615000000000002 26.465 20.365 85-89 24.51 28.845 25.759999999999998 20.885 90-94 24.175 28.89 25.915 21.02 95-99 24.45 29.195 26.340000000000003 20.015 100-104 25.385 28.705000000000002 25.729999999999997 20.18 105-109 25.085 27.875 26.3 20.74 110-114 25.19 28.775000000000002 25.905 20.13 115-119 25.085 28.794999999999998 26.375 19.744999999999997 120-124 25.330000000000002 28.435 26.845000000000002 19.39 125-129 24.945 28.92 26.145000000000003 19.99 130-134 25.465 27.825 26.38 20.330000000000002 135-139 25.83 29.015 25.564999999999998 19.59 140-144 26.229999999999997 29.12 25.535000000000004 19.115 145-149 25.779999999999998 28.345 26.279999999999998 19.595000000000002 150-151 26.8 28.4375 26.337500000000002 18.425 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.5 8 1.5 9 1.0 10 0.5 11 0.0 12 0.0 13 0.0 14 0.5 15 1.0 16 1.0 17 1.0 18 1.5 19 2.0 20 1.0 21 0.0 22 2.5 23 4.5 24 3.0 25 2.5 26 3.0 27 2.0 28 8.5 29 13.5 30 6.5 31 9.5 32 13.5 33 14.0 34 25.5 35 33.5 36 58.5 37 88.5 38 103.0 39 143.0 40 192.5 41 210.0 42 236.0 43 258.0 44 244.5 45 280.0 46 300.0 47 279.0 48 249.0 49 206.0 50 186.0 51 155.0 52 121.0 53 89.5 54 86.5 55 73.5 56 41.5 57 34.5 58 32.5 59 25.0 60 19.5 61 16.0 62 10.0 63 13.5 64 13.0 65 6.5 66 5.5 67 6.5 68 6.0 69 5.5 70 2.0 71 1.5 72 3.0 73 3.0 74 2.0 75 1.5 76 2.0 77 1.5 78 1.5 79 2.0 80 2.0 81 2.0 82 2.0 83 1.0 84 0.5 85 1.5 86 1.5 87 2.0 88 2.5 89 2.0 90 1.5 91 0.5 92 0.5 93 0.5 94 0.5 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.775 #Duplication Level Percentage of deduplicated Percentage of total 1 60.24204392649036 33.6 2 20.75302554908113 23.150000000000002 3 9.143881667413716 15.299999999999999 4 5.154639175257731 11.5 5 2.0618556701030926 5.75 6 0.9861048857014791 3.3000000000000003 7 0.8964589870013447 3.5000000000000004 8 0.44822949350067237 2.0 9 0.17929179740026896 0.8999999999999999 >10 0.1344688480502017 1.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 18 0.44999999999999996 No Hit CTCAGTGGCGTAACTTGGGTTACCAGAATCAGCCAGAGCACCAGGCATTT 12 0.3 No Hit GGTGATTCCTCAAGATGATCTTGTTTTGCTTGCAATTATTGGCATAAAGG 10 0.25 No Hit CCCAAGTCCTGTGTTACCAGTTTCAGCAGTAACATGTTGGATTTCTCTAC 9 0.22499999999999998 No Hit CCGATACAGCAAGTGCTTCTGTAGCTTTGACATCTTTGTCAAATGAAAAG 9 0.22499999999999998 No Hit CAACAATCTTTGTGCAGTTTCCGCTACAGGCAAAGGGGAAACAATTATAC 9 0.22499999999999998 No Hit CTCTTCTTTCTCTCTAATTTACATCTCCATTGTTGCATAGAAACTCTCTG 9 0.22499999999999998 No Hit ATGAATTCCGCCCAAAACAGTTGCTTAAGCAGATTGTCCATATATATGTT 8 0.2 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 8 0.2 No Hit TATAGAGATGGTTTTGGGGAAGGCAGACGTTCCTATAGCGAAGCACTACG 8 0.2 No Hit AGTAGCTGATGTTGATAAAGCTGACATGGGTGCCTCAGGTGAATTCAAAG 8 0.2 No Hit AGCGTGGTATACTGTTAATTTAGTGGTATTCTTCCTGGACAAGAAATTAC 8 0.2 No Hit GGAAGATGAGGGTCGTAGAGGATAGTGCTGGTGGTGGTGCCAAGCCCAAA 8 0.2 No Hit GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA 8 0.2 No Hit GTTACGAACTTTTTTGCTGTTAGCAGCAGATCTGGAAATCCTGAAGACCT 8 0.2 No Hit AGCGGGACACTAAACGGTTCTTCTATTGTATATGTCAAGACAGGGAGTCC 8 0.2 No Hit GTGGAGGCCATCAATGTGGCTGCAACAGACTGGGGTCTCCGATGTCTTCG 8 0.2 No Hit GGGAGTATCTAGATACTCGGCCTGATGGATGGTTGGATTATGCAGCTTTA 7 0.17500000000000002 No Hit AGAGGTTTCAGTGGTTTCTCTAGATGAAGAATTTGTGGATTTCACTGACA 7 0.17500000000000002 No Hit GCCATGGCAATCCAGGCGGTCAGAGTTCATGATTTGTTGGAAGCAGAGAT 7 0.17500000000000002 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 7 0.17500000000000002 No Hit CTTGCATCTCGGTTGATGGCCCGCTCATCACCCAGAGTAGAGAGCTACAT 7 0.17500000000000002 No Hit AGAATGTCGAGTGGTGGCACAAAGTTTACTTCTGGGCTTCAAAGACAGTC 7 0.17500000000000002 No Hit GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG 7 0.17500000000000002 No Hit AGAAAATGAAAGCCAGCACCAATGTAAGGCATATTGTACTTCCCTGTTCT 7 0.17500000000000002 No Hit CAAATGGGCATGTTGAATGCCAGTGGTGTAGGGGTACAGGGTTCTTCATT 7 0.17500000000000002 No Hit ACTTGTAGCATGCTCTTCTTCTTACATTATGTCAGAGTCAAAGCTCAAAA 7 0.17500000000000002 No Hit GTTCACCAAAGCCAAGGTAGCCAGCAGAAGTAAAGAGACAGAGAGACATA 7 0.17500000000000002 No Hit GAACCTTCCAGCATGGTCTGGCATATACCCTGGTTTATTATCAAGCTACG 7 0.17500000000000002 No Hit GAGAAAGGTAAACTGGTATTTGGAGATTTCAATTATCCAGAGAATGGAGA 7 0.17500000000000002 No Hit GAAAGATAAAGGGCCAGTGTGGGAGGAGATCGTGAAGAAGAACCAGTTGT 7 0.17500000000000002 No Hit GTTGAAGATGTGATGATGAAATCCTTTGTCCGGACAAGCTTGAGCCATCT 7 0.17500000000000002 No Hit ATAAAGGCTCACGCAACAAGAAGTTCGTTGATAGAACTTTCATGGCCATA 7 0.17500000000000002 No Hit CTCCTGTTCATCCCCTCTCTCTCTTTCTGCGTTCTAGATCCTTCCATTCC 7 0.17500000000000002 No Hit CAACAACAACGTCAGTAGCAGCAGTGAATACGAACGCCAACATCAACACG 7 0.17500000000000002 No Hit GTTGGTTCCTAACATACAAAATTTCTTTCTTCTCTGCGCGATCATCCTCT 7 0.17500000000000002 No Hit AACAAAACAAAGCAAAACCCTATATTTGGCCATAGTAGAAAAGAGAGAAT 7 0.17500000000000002 No Hit CATCTTAACTGCTACTTTTCCTTTCCTTCCCCCCTTATAATTCCCATTTC 6 0.15 No Hit GAAAAGTGAAAACGCACATTCAGACGCCTCTATCTCCACCTCCCTCCCTC 6 0.15 No Hit TGGTCATGAGATCACTGGAGAAGTTGTGGAACATGGGGAGCTGACAGATA 6 0.15 No Hit CATTAATCTTCATTGATTCAATTAAACCTTGCTCTTTTGCTAAGAAACAA 6 0.15 No Hit TGCAGCGAGAAAGGGTGTGATGCCCAAGGACTTTCACAGATGAAGTATAG 6 0.15 No Hit AGTGGTTGCAGCAGTTCTTGAGGGTCGTGCTGGGAGGAATTGCACGGTTG 6 0.15 No Hit GGTTTCTTAAACTCGATGCTCGAGCAAGAGAGGACACTGAGAAAATTGAC 6 0.15 No Hit GTTCATCCGCACCGGCACAGCAGGTTACGTCGTGTGCTACGGCGAGATGG 6 0.15 No Hit AAAGCATTCAAGCTGGACTTGGAAGCTGTCAGACCAGATCCTCTAATTCC 6 0.15 No Hit GAAGAATCCTTGACTTGGTGGACAAGAAATTAGCATCCAGTTACGACAGG 6 0.15 No Hit GAAAGGAGACACCCAAATCTTAGCTTGCCCTAACTTCCTCTTCGTCCATA 6 0.15 No Hit GTAATGTGTGCTTGCACATGTTTACTGTGGATTTTCTAAATCAAGTGGCA 6 0.15 No Hit ATTTTGAAAATTAGGGTTCCCCTACTCTGCTTCTCTTTCACTCGCAAGCC 6 0.15 No Hit GGGAGAAAGAAAAGATAGCCTTCTTCACGAGAAAAAAGATGAGGAACATC 6 0.15 No Hit AAAGGAGGCGAGTTGTTTTCCAAGATTTCGAAAGGGAGGTTCAGTGAAGA 6 0.15 No Hit ATTTCAGATGGGTCTCGTAGGTATGGTGACCAACCTATGCAATATCAGGA 6 0.15 No Hit TAATAACCCTAACCACAAACCTTAGAGTTCTCTGTTCGGGTTCGGATTCG 6 0.15 No Hit CAAAACAATGGCCACCTCGAAGCTTCTAGCTTCCACCCTCCGCAGAACCC 6 0.15 No Hit GGAAAATATTGGTGATAGCAGCAGGATGCTATATCCTTGTAATGTCTTGT 6 0.15 No Hit GGCCACCGAAGACGTTTCTCTCGACCTTTCAAAGCTTCTTTCTTCAGAAG 6 0.15 No Hit GTGCCTTTTACACTTGAGAAGTCGGAATTGGAGGCTGAGTTCATCAAAGA 6 0.15 No Hit GTCCAAGATCCCTTTCATAGATTGTTGCTGCATGGCGTCTGTGAGTTCTA 6 0.15 No Hit CAAGAAGGGGGTCAAGACCAGAGGAGAGATTATGCTTGCAGTTTGGATGG 5 0.125 No Hit GTTGAATGTTGCGTAGAGCTCGACTCCTTTCTCAGCAAACCTTTGCTAGT 5 0.125 No Hit GTGATGGCTACATATACCAAGATATTCTTGCAATTCGATCACGATTTCTG 5 0.125 No Hit CTCTTGGGCTAGCACTTCCTGCGAGATGATACGAATTTGGGTAACTCGGC 5 0.125 No Hit ATACGCCCTCCTTTTTCTTCCTCCTCCTCCTCCATGGATGGTGAAGTGGG 5 0.125 No Hit AGGTGGAAAGATGGAGGTATGGTGCATCAACGGCAGTGCAAAGACTCCAT 5 0.125 No Hit CGAGGAGGAGGAAGAGGAGGCGATTGGAGAAGCCAAAGAGTCGGCGCCAA 5 0.125 No Hit CCAATCTAGGGTTTTAGCTCTTTGCTTCTCTCTGTCTCTCCGTACTCTTC 5 0.125 No Hit CACCTAAACATTATCAAAGCTTCTTTCCTGGTACCATTTCTGGCACTGTT 5 0.125 No Hit CTTTGTTGAACTTCCAGACACATGTCAAGGTCTATACTATTGCAACATCT 5 0.125 No Hit CCGGGTTTGATTTTCGATTCCAGTTATGATGACTATATGTCATTTCTTTG 5 0.125 No Hit AGGCAAGAAACTCCTACCTGCTTCAAAATAAACTGCTAAGTAACTTTTCA 5 0.125 No Hit ATTCCGATCAATTTTGTTACTTTCTTATCACAGGGAATTTGTAAACAGCG 5 0.125 No Hit GCTGTAGAGCTAGCTATTGTGTGTGTCGCCGCAGCAGTAGAATAAGAAGG 5 0.125 No Hit GCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAAT 5 0.125 No Hit CTTGAAAAGATGCCGCAAGGAATGGAATGCCTAACCAACCTGAGGTATCT 5 0.125 No Hit GCTCGAAGTACCTCTACACCCTTTGTGTCTTTGACACTGAGAAGGCAGAC 5 0.125 No Hit GAAAGTTCCAGCACATAAAACTGAAGAGGTCCCTCCTCCAGCTGAATCTG 5 0.125 No Hit GTGAAATCCCAGGCTCCTTTGAGAAATTATCTAGCTTGCTTGGATGCAAC 5 0.125 No Hit AATGAGTGATGGGTTTTTGGTGAATACTGGTTTACAAGCCAAAACAAGAA 5 0.125 No Hit GTCCCTCCCCATCCAGCTAAATCTGCTTCTAATGAAGGTGCATTTTCTCA 5 0.125 No Hit TCAGCATCGTTTGACAGAATTTCGTGCACAGAATCAAGCTCCTGGACAGC 5 0.125 No Hit CCTTTATCCTAATGCACATCCTTTGGCAATTGATCTGCTACAAAAAATGC 5 0.125 No Hit AGAAGTTCGCACGTAGCATACAAACGACGAAGCCCCAAGGTGGTTTTTGC 5 0.125 No Hit GGTGAACTTGAGTTGCCGTTTATGGCGTTGCTCTTGGTAATTGTGGCTCC 5 0.125 No Hit CATCGGAAGGGGAAGCCGAAGATGCAGCAATCATATCAAGATTCCAACAA 5 0.125 No Hit GTTGTTGGAAAAACTTCAGAAGTTCTACATTCTGCTGTCATCACTTGTTT 5 0.125 No Hit CTATCGCCGCTTCAGCTTGTGCGTAGCAACAGCCGCCGGTTTATGCCATC 5 0.125 No Hit AATCAATCAAATGTTCTCTGCTTTAACTGCAACTCTTGCAAGGCTGGGCT 5 0.125 No Hit GAGAAATGGTGCCCGTTTTCAGAGGACTAGTAGACCCACTTCCTTTGTTG 5 0.125 No Hit AGACTTGTGACCCAGAGTGGAGATCATTGATGGAGAGATGCTGGTCTTCT 5 0.125 No Hit GTGGTTCTATCAGTATATCTGTCAATGTCATCTGCTGCCTGGGAACAACT 5 0.125 No Hit GTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTG 5 0.125 No Hit GGTTTTCTCTCGAAGAACTCAAGGCTGCTGGTATTCCAAAGAAACTAGCT 5 0.125 No Hit TGCTTTAACATATCAAGAAAACAGAGGTACTGAAGAATACTTGGACAATA 5 0.125 No Hit CAGAAAAACAGCTTCTTACTCGTCGTATGGTGTTCTTTTTTTTTTTTTTT 5 0.125 No Hit AGAGAGATAAAAACAAATGAGCGTCTCCTTTCTCCTCCTCCGCAAGAGTA 5 0.125 No Hit TATGGAATAATTGATGGAGTTATTGATAGAGATAGCATCATTCCTTTGGC 5 0.125 No Hit GTGGAACGCTCATGATGTTCCACATGAAGGAAATCTACACCTTCACACAT 5 0.125 No Hit TGCGAAAGCAGGTAAGATGCCAGTTATAATTGAAGAGCGTATGCAGAAGG 5 0.125 No Hit GGATGCCCCTGATGAAGAACACTTCCAATACTGAAGTTCTGCCTGCCTTT 5 0.125 No Hit GATTAATCTGTTGAGACAACTTCAGCAAATGCTACCACCTGCTACCCTAG 5 0.125 No Hit CCAAGGACATCATTTAAAGGAGTAGTTGTTTTCAGATACCATACAACAAG 5 0.125 No Hit TGAGACTAATGCAGCTTCTGTTTAACCTTTTGTTTTCGGCCCCCTTTTGA 5 0.125 No Hit GGGACACCATTGGAAGTGATGGATCCAACTCTGGCGGATACGTATTCAAG 5 0.125 No Hit CAAGATCTTGGCAAAAGATAGCGGTCTCCATTCACTGCATACATCACAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.0625 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.21250000000000002 0.0 0.0 0.0 0.0 82-83 0.2875 0.0 0.0 0.0 0.0 84-85 0.4 0.0 0.0 0.0 0.0 86-87 0.45 0.0 0.0 0.0 0.0 88-89 0.6000000000000001 0.0 0.0 0.0 0.0 90-91 0.775 0.0 0.0 0.0 0.0 92-93 1.0499999999999998 0.0 0.0 0.0 0.0 94-95 1.1 0.0 0.0 0.0 0.0 96-97 1.2 0.0 0.0 0.0 0.0 98-99 1.425 0.0 0.0 0.0 0.0 100-101 1.6625 0.0 0.0 0.0 0.0 102-103 2.075 0.0 0.0 0.0 0.0 104-105 2.175 0.0 0.0 0.0 0.0 106-107 2.375 0.0 0.0 0.0 0.0 108-109 2.6624999999999996 0.0 0.0 0.0 0.0 110-111 3.0625 0.0 0.0 0.0 0.0 112-113 3.4125 0.0 0.0 0.0 0.0 114-115 3.7875 0.0 0.0 0.0 0.0 116-117 4.199999999999999 0.0 0.0 0.0 0.0 118-119 4.675 0.0 0.0 0.0 0.0 120-121 5.4875 0.0 0.0 0.0 0.0 122-123 5.775 0.0 0.0 0.0 0.0 124-125 6.1875 0.0 0.0 0.0 0.0 126-127 6.6375 0.0 0.0 0.0 0.0 128-129 7.0625 0.0 0.0 0.0 0.0 130-131 7.3375 0.0 0.0 0.0 0.0 132-133 7.8875 0.0 0.0 0.0 0.0 134-135 8.6 0.0 0.0 0.0 0.0 136-137 9.537500000000001 0.0 0.0 0.0 0.0 138-139 10.2625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCAAGC 10 0.006830828 145.0 5 AGGAAAA 10 0.006830828 145.0 6 TCGTGGT 10 0.006830828 145.0 4 GTGAGTC 10 0.006830828 145.0 3 GAGTCGT 10 0.006830828 145.0 5 CGTGGTG 10 0.006830828 145.0 5 ATGTGAG 10 0.006830828 145.0 1 AGTCGTG 10 0.006830828 145.0 6 TGAGTCG 10 0.006830828 145.0 4 >>END_MODULE Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra Read 1060536 spots for SRR26075405.sra Written 1060536 spots for SRR26075405.sra Read 1060535 spots for SRR26075405.sra Written 1060535 spots for SRR26075405.sra SRR ids: ['SRR26075405.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_9txgmjth SRR26075405.sra spots: 21210701 blocks: [[1, 1060535], [1060536, 2121070], [2121071, 3181605], [3181606, 4242140], [4242141, 5302675], [5302676, 6363210], [6363211, 7423745], [7423746, 8484280], [8484281, 9544815], [9544816, 10605350], [10605351, 11665885], [11665886, 12726420], [12726421, 13786955], [13786956, 14847490], [14847491, 15908025], [15908026, 16968560], [16968561, 18029095], [18029096, 19089630], [19089631, 20150165], [20150166, 21210701]] SRR26075405 file size 7828530 SRR26075405 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075405 SRR26075405_1.fastq SRR26075405_2.fastq Input file: SRR26075405_1.fastq Paired file: SRR26075405_2.fastq trimmed: SRR26075405-trimmed-pair1.fastq, SRR26075405-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:04:45 2025 >> started Wed Feb 12 02:05:10 2025 >> done (25.152s) 21210701 read pairs processed; of these: 97 ( 0.00%) short read pairs filtered out after trimming by size control 42437 ( 0.20%) empty read pairs filtered out after trimming by size control 21168167 (99.80%) read pairs available; of these: 3510739 (16.58%) trimmed read pairs available after processing 17657428 (83.42%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 14 0.00% 19 11 0.00% 20 11 0.00% 21 8 0.00% 22 15 0.00% 23 17 0.00% 24 7 0.00% 25 17 0.00% 26 20 0.00% 27 19 0.00% 28 31 0.00% 29 28 0.00% 30 21 0.00% 31 22 0.00% 32 37 0.00% 33 28 0.00% 34 64 0.00% 35 34 0.00% 36 26 0.00% 37 29 0.00% 38 41 0.00% 39 45 0.00% 40 57 0.00% 41 82 0.00% 42 45 0.00% 43 57 0.00% 44 71 0.00% 45 78 0.00% 46 72 0.00% 47 93 0.00% 48 107 0.00% 49 99 0.00% 50 129 0.00% 51 132 0.00% 52 146 0.00% 53 198 0.00% 54 168 0.00% 55 178 0.00% 56 209 0.00% 57 255 0.00% 58 304 0.00% 59 304 0.00% 60 368 0.00% 61 497 0.00% 62 450 0.00% 63 578 0.00% 64 595 0.00% 65 687 0.00% 66 695 0.00% 67 842 0.00% 68 847 0.00% 69 969 0.00% 70 1203 0.01% 71 1444 0.01% 72 1593 0.01% 73 1867 0.01% 74 2194 0.01% 75 2467 0.01% 76 2556 0.01% 77 3002 0.01% 78 3477 0.02% 79 3830 0.02% 80 4554 0.02% 81 4793 0.02% 82 5456 0.03% 83 6270 0.03% 84 7116 0.03% 85 8145 0.04% 86 8588 0.04% 87 9680 0.05% 88 10313 0.05% 89 11329 0.05% 90 11699 0.06% 91 13155 0.06% 92 14145 0.07% 93 15874 0.07% 94 16959 0.08% 95 18676 0.09% 96 20449 0.10% 97 21777 0.10% 98 22617 0.11% 99 23903 0.11% 100 25319 0.12% 101 25990 0.12% 102 28116 0.13% 103 29824 0.14% 104 31649 0.15% 105 33501 0.16% 106 35094 0.17% 107 37059 0.18% 108 37885 0.18% 109 39266 0.19% 110 40073 0.19% 111 41294 0.20% 112 43406 0.21% 113 44564 0.21% 114 46618 0.22% 115 48922 0.23% 116 50545 0.24% 117 52040 0.25% 118 54828 0.26% 119 55535 0.26% 120 55772 0.26% 121 58427 0.28% 122 58316 0.28% 123 60219 0.28% 124 62646 0.30% 125 63529 0.30% 126 66483 0.31% 127 68558 0.32% 128 69355 0.33% 129 70790 0.33% 130 73073 0.35% 131 73344 0.35% 132 74919 0.35% 133 75697 0.36% 134 76332 0.36% 135 78861 0.37% 136 80132 0.38% 137 80423 0.38% 138 83884 0.40% 139 84501 0.40% 140 85002 0.40% 141 86119 0.41% 142 88344 0.42% 143 88421 0.42% 144 90015 0.43% 145 91676 0.43% 146 92916 0.44% 147 93849 0.44% 148 95381 0.45% 149 94574 0.45% 150 98665 0.47% 151 17657428 83.42% 21168167 reads passed initial QC criterion=sequence-density sequence-density=0.27 sequence-density-rank=1 fanout-score=2.97 fanout-score-rank=24 prefix-density=0.30 prefix-fanout=2.7 sequence=AGGAAACCTCCT criterion=fanout-score sequence-density=0.05 sequence-density-rank=30 fanout-score=385.21 fanout-score-rank=1 prefix-density=0.76 prefix-fanout=25.0 sequence=TCATCATCACCACCATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTC criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=2.66 fanout-score-rank=27 prefix-density=0.35 prefix-fanout=2.5 sequence=TGGTTTTACTAG criterion=fanout-score sequence-density=0.11 sequence-density-rank=19 fanout-score=67.84 fanout-score-rank=1 prefix-density=0.43 prefix-fanout=17.3 sequence=TGAAGAAAAGAA SRR26075405 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:05:54 Started mapping on | Feb 12 02:05:54 Finished on | Feb 12 02:10:21 Mapping speed, Million of reads per hour | 285.41 Number of input reads | 21168167 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 18505819 Uniquely mapped reads % | 87.42% Average mapped length | 292.49 Number of splices: Total | 17736078 Number of splices: Annotated (sjdb) | 17290177 Number of splices: GT/AG | 17391482 Number of splices: GC/AG | 267337 Number of splices: AT/AC | 20587 Number of splices: Non-canonical | 56672 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.04% Deletion average length | 3.68 Insertion rate per base | 0.02% Insertion average length | 2.59 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 467766 % of reads mapped to multiple loci | 2.21% Number of reads mapped to too many loci | 76362 % of reads mapped to too many loci | 0.36% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 9.68% % of reads unmapped: other | 0.33% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2194582 2194582 2194582 N_multimapping 467766 467766 467766 N_noFeature 494439 18316575 605850 N_ambiguous 180087 975 101685 UnstrandedReadsAssigned:17831293 PositiveStrandReadsAssigned:188269 NegativeStrandReadsAssigned:17798284 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075405 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075405-trimmed-pair1.fastq SRR26075405-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 21,168,167 reads, 18,028,040 reads pseudoaligned [quant] estimated average fragment length: 211.068 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,043 rounds 52401 SRR26075405.ke.tsv 34699 SRR26075405.se.tsv 87100 total ==> SRR26075405.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1807.93 2099 62.447 Potri.005G024800.1.v4.1 1035 824.932 1505 98.1295 Potri.004G059700.1.v4.1 961 750.932 0 0 Potri.007G009000.2.v4.1 1416 1205.93 0 0 Potri.003G141000.2.v4.1 2943 2732.93 1017.45 20.0247 Potri.016G087400.1.v4.1 270 90.8462 1265 748.971 Potri.015G069301.1.v4.1 564 355.82 0 0 Potri.010G195200.1.v4.1 1773 1562.93 563 19.3754 Potri.012G127500.1.v4.1 977 766.932 18913 1326.43 ==> SRR26075405.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 42 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 194 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 8 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 962 SRR26075405 completed mapping pipeline successfully