Starting /dee2/code/volunteer_pipeline.sh SRR26075406 current disk space = 3051189903360 free memory = 1298265812 SRR26075406 SRAfilesize 034cbf528ef1e194189c6f408d011a28 SRR26075406.sra SRR26075406.sra file validated SRR26075406 is paired end SRR26075406 is conventional basespace SRR26075406 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075406_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.63875 37.0 37.0 37.0 37.0 37.0 2 36.5695 37.0 37.0 37.0 37.0 37.0 3 36.6675 37.0 37.0 37.0 37.0 37.0 4 36.659 37.0 37.0 37.0 37.0 37.0 5 36.738 37.0 37.0 37.0 37.0 37.0 6 36.6995 37.0 37.0 37.0 37.0 37.0 7 36.643 37.0 37.0 37.0 37.0 37.0 8 36.6865 37.0 37.0 37.0 37.0 37.0 9 36.6955 37.0 37.0 37.0 37.0 37.0 10-14 36.656099999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.601099999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.5505 37.0 37.0 37.0 37.0 37.0 25-29 36.451299999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.3954 37.0 37.0 37.0 37.0 37.0 35-39 36.355500000000006 37.0 37.0 37.0 37.0 37.0 40-44 36.3211 37.0 37.0 37.0 37.0 37.0 45-49 36.2816 37.0 37.0 37.0 37.0 37.0 50-54 36.289300000000004 37.0 37.0 37.0 37.0 37.0 55-59 36.19350000000001 37.0 37.0 37.0 37.0 37.0 60-64 36.1459 37.0 37.0 37.0 37.0 37.0 65-69 36.051 37.0 37.0 37.0 37.0 37.0 70-74 36.09159999999999 37.0 37.0 37.0 37.0 37.0 75-79 36.0403 37.0 37.0 37.0 37.0 37.0 80-84 36.0371 37.0 37.0 37.0 37.0 37.0 85-89 35.831199999999995 37.0 37.0 37.0 37.0 37.0 90-94 35.8012 37.0 37.0 37.0 37.0 37.0 95-99 35.7659 37.0 37.0 37.0 37.0 37.0 100-104 35.840999999999994 37.0 37.0 37.0 37.0 37.0 105-109 35.6356 37.0 37.0 37.0 37.0 37.0 110-114 35.649699999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.5407 37.0 37.0 37.0 37.0 37.0 120-124 35.47280000000001 37.0 37.0 37.0 37.0 37.0 125-129 35.4002 37.0 37.0 37.0 37.0 37.0 130-134 35.21489999999999 37.0 37.0 37.0 29.8 37.0 135-139 35.1206 37.0 37.0 37.0 29.8 37.0 140-144 34.9277 37.0 37.0 37.0 25.0 37.0 145-149 34.8883 37.0 37.0 37.0 25.0 37.0 150-151 34.61175 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 5.0 22 1.0 23 2.0 24 4.0 25 13.0 26 11.0 27 12.0 28 20.0 29 27.0 30 35.0 31 46.0 32 70.0 33 102.0 34 156.0 35 466.0 36 2842.0 37 187.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.86275982970198 14.274981217129978 9.366391184573002 40.49586776859504 2 18.3 14.85 34.2 32.65 3 17.65 18.6 27.425 36.325 4 20.225 23.9 25.05 30.825000000000003 5 24.675 25.95 25.724999999999998 23.65 6 21.15 30.95 25.074999999999996 22.825 7 17.275 26.75 41.075 14.899999999999999 8 16.7 26.974999999999998 32.425 23.9 9 17.299999999999997 24.95 34.575 23.175 10-14 19.096909690969095 28.972897289728973 28.312831283128315 23.617361736173617 15-19 20.64 26.945000000000004 28.32 24.095 20-24 20.36 27.82 27.900000000000002 23.919999999999998 25-29 19.865 28.54 28.27 23.325000000000003 30-34 20.105 27.41 28.305000000000003 24.18 35-39 19.78 28.34 27.345000000000002 24.535 40-44 20.31 26.669999999999998 28.275 24.745 45-49 20.005 27.485 27.605 24.905 50-54 19.935 26.895000000000003 28.935 24.235 55-59 20.5 27.305 28.144999999999996 24.05 60-64 20.599999999999998 27.205000000000002 27.755000000000003 24.44 65-69 19.915 26.834999999999997 28.835 24.415 70-74 20.424999999999997 28.065 27.295 24.215 75-79 20.169999999999998 27.355 27.884999999999998 24.59 80-84 21.215 26.779999999999998 27.529999999999998 24.474999999999998 85-89 20.724999999999998 27.279999999999998 27.915 24.08 90-94 20.895 27.185 27.43 24.490000000000002 95-99 20.595 26.740000000000002 28.804999999999996 23.86 100-104 21.29 27.71 27.155 23.845 105-109 20.34 28.249999999999996 27.229999999999997 24.18 110-114 20.89 27.815 27.200000000000003 24.095 115-119 20.785 27.07 27.169999999999998 24.975 120-124 21.855 26.615 27.165 24.365000000000002 125-129 21.215 26.775 28.03 23.98 130-134 21.395 27.689999999999998 26.605 24.310000000000002 135-139 21.595 26.119999999999997 27.015 25.27 140-144 21.415 26.91 27.07 24.605 145-149 21.715 26.640000000000004 26.540000000000003 25.105 150-151 22.3875 28.275 24.675 24.6625 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.5 8 0.5 9 1.5 10 1.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 1.0 20 0.0 21 1.0 22 1.0 23 0.5 24 1.0 25 2.0 26 4.0 27 3.5 28 5.5 29 14.5 30 22.0 31 31.0 32 28.0 33 20.0 34 29.0 35 48.0 36 85.0 37 115.0 38 129.0 39 140.5 40 195.0 41 227.5 42 210.5 43 236.0 44 243.0 45 255.0 46 262.5 47 249.5 48 205.5 49 199.0 50 215.5 51 165.0 52 131.5 53 114.5 54 94.5 55 66.5 56 48.5 57 35.5 58 29.0 59 28.0 60 14.0 61 10.5 62 11.0 63 5.5 64 12.5 65 13.5 66 7.5 67 6.0 68 7.0 69 5.0 70 2.0 71 1.5 72 1.5 73 2.0 74 1.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17500000000000002 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.699999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 62.180579216354346 36.5 2 20.272572402044293 23.799999999999997 3 9.497444633730835 16.725 4 4.301533219761499 10.100000000000001 5 1.9591141396933562 5.75 6 1.0647359454855196 3.75 7 0.46848381601362865 1.925 8 0.08517887563884156 0.4 9 0.04258943781942078 0.22499999999999998 >10 0.12776831345826234 0.8250000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGAAACTTGGGCAAAGGAGGACCTAGATTGTTTTCACTGACATCGAACAA 13 0.325 No Hit CTCCGGAATTTCCGATGCAGGTTGTGCATCCATAACCAACAATATTAAAC 10 0.25 No Hit CGTGGTAGGATTTACAACTTTTGACAGAACAAATATAACACTTTTACAAC 10 0.25 No Hit ACCAGCCGTCTGGTAAACATAGGTAAGCCCACACTTACCACAGTAGTGCC 9 0.22499999999999998 No Hit CTGTTCTTCTTGTTATCCCTTGCTGCATTTCCAGCTAGCTCTAAAACCTC 8 0.2 No Hit GCTCATTCCGTCTCTATTGCCAAAACCGCTGCTCATTCCATCTCTATTGC 8 0.2 No Hit GTCGAAATATAGTCATGGTAATCACCGGACGTGGTGTTCGAGCCTTGCCG 7 0.17500000000000002 No Hit CCGGAAGTTGAACATCACTTTCCATGGCTGACGTGTGCCCAGACCAGCTT 7 0.17500000000000002 No Hit ACCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCT 7 0.17500000000000002 No Hit ACATAACTTACTCATTCCTACATTTATTTAACACTCCGTGTTTAATACAT 7 0.17500000000000002 No Hit TGGACTTCTGTCTGTAGGGGCGAAATTTTCTGAGAAAGACTTTATCCTGT 7 0.17500000000000002 No Hit CTCTTTTCAATTGCCTTCGTATCAACCTCCCCTGAGATTGTGAATAGAGA 7 0.17500000000000002 No Hit GCGAGTTTGCTAAGAAGAAGTTTGGACACTCCAACATGAGCTCTGCTGCT 7 0.17500000000000002 No Hit CCGGCAACCAAATGCATTTACTTCCCTTCTGTATTTGTCTCTTCTATCTC 7 0.17500000000000002 No Hit CTCCGCTGCATATCGATCTCCGATCAAACCAGATAGCAGACCGTTAAATC 7 0.17500000000000002 No Hit CCAGCAAATGCATATCCAGCACCTAATCCACCAAAATGCAAGGAATAATT 7 0.17500000000000002 No Hit CCTTGATGTCTAGTCTCTCTTTCTTTGGGCCCACTAATACACTCGTGTCC 7 0.17500000000000002 No Hit CTCTGACATTGACTTCTCAGACTCCTCTTCTTCCTTGGGCCCTTCAGCCT 6 0.15 No Hit GTTCGAAACTAGATGAAATGGGTTAAATAATTCGTGAAATGATTTAGGAA 6 0.15 No Hit GCAATTTATCCGACTAGAAGGGAAGAGGAGGCGGCCGCGGCAGTGTCCTT 6 0.15 No Hit TACCATGCGAACCCGTTTTATTGGTCCATAAGATTCAAACTCCCTTTTAA 6 0.15 No Hit CTAGCTGTTTCACTACCCCAAGCATTCTGACAGACCATTCTCCAGAGATC 6 0.15 No Hit CCCGGATCTCTTCCTCCTTGGCTGGAACTGCAGTGATTTTGTTTTTAGGA 6 0.15 No Hit CTCTCATTGGGTTTTTCACCTGGAGCATCTATCTCAGAAACCTTTCCAAA 6 0.15 No Hit CCTGTCCAAACTGCTGCCATGCCCACTCTGCAAGGCTGGTATGCTCATCT 6 0.15 No Hit CGTTTCTGAACAACGGAAGTCAGCTCTCTGATCCTCCTTCCCTTCTCACC 6 0.15 No Hit TGGGCTTGCTTGCACTCTTGTACATGTTGTCGATAACATTCTGGTAGTAT 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTCCTTATCTCGTAT 6 0.15 TruSeq Adapter, Index 21 (97% over 38bp) GCACAGTTCACTGTTGCTGCCACTGGCAGACCCAGTGACATCCTGAACTT 6 0.15 No Hit GGCATGGCATTTCCTTTGCTTCAGATCCAATCTCAAACTCTTCCAAGCAT 6 0.15 No Hit GAGAAAAATATAACTAGAGTACCACAAACATTAAGAATAAAACTATCGAG 6 0.15 No Hit CCATGACAGTCAAGACAGTGATGTAAAGGAACAAGAAAGTGGCTATGAAC 6 0.15 No Hit CAGCCTGTGACCGCAGCTGCCACTTCTCAGAGTTGTGTTCCTGCAACTGA 6 0.15 No Hit TGTAGAAATAAGGGAGCAACATGTACCGAACACGTATGGCCTCTCTTATT 6 0.15 No Hit GGAGTTTTGGGCTAACTTCGGGTTGATTTGAGTATTGATTTACGGGTGAA 6 0.15 No Hit GCCTGTCTTAAACCGCTCCTCGAACCTAGCCTTTGTCTCCTTGCAAGCAG 6 0.15 No Hit CTTGGTTTCCACTGCTCATAAGCCCACCATTGATTTGCCTGCCTCCAACA 6 0.15 No Hit CGCTGCTTTTTGAGCTTCCACTATTACTGGTGGTCAATCCAGCACCATTC 6 0.15 No Hit AGCCGATCATCCTCTTCATCTGATGACTCGTTCTTCTTCCTCCTTTTCTC 6 0.15 No Hit GTTTTCGTATGGGTGGTCGATATTCTATTTGTTCTTCTGGAGGCGATGGT 6 0.15 No Hit CAGGTTAAAAGGGTCAAAAAGAAGAAGATCACAGTCAATGGCCAGGCTTT 6 0.15 No Hit AAGCCATTCCTTCCACCACGAAGCCGACTGAAATTGTAAAATTCGTCACC 6 0.15 No Hit CTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGA 5 0.125 No Hit GAGACGCGGGTAGTACAATGATGTTTACACTAATTAACCATTTTTACATT 5 0.125 No Hit GGAACCCTAAATAACCTTACTAAGAAAAAAGAGAGAATAAAACAACATAA 5 0.125 No Hit CGGTCACTGTCATGGCTTCTGGGATCCCCATTGTGGACAACTAAGCTACT 5 0.125 No Hit CGATCTTCTACATGACTATTGTTGTTATCTTTATTACAAACATTGTCTCC 5 0.125 No Hit CACCGGGCCTGCAGTGTTCCCTCCAATGGCAGCCATAACCTGGCTAAAAA 5 0.125 No Hit CCCAGTTAAGTTCCAACCAACCATGGAACCCTGAAATAAAAGGGGGGAAA 5 0.125 No Hit CCTTGACCTGATCACCATTGTTGCGGATGAGAAAGTCCCTCTCTTCTGAA 5 0.125 No Hit CCCATCACTCTCCGTCAACACCGGGGACGGTGTCTCAGACTTGACCACCG 5 0.125 No Hit ATGCACAGAAACCATCGGAAACTTAAGAATCCAAACAAAACTTTCATTGG 5 0.125 No Hit CATCCTTCTTCCCCCTTTTCTCCATTGTTGGCACCTCGCGAGGAATGAGT 5 0.125 No Hit CTCCAAGACCATAGATTTTTCTGGTACAACAGGGGCAGCAGAAGAAAAAT 5 0.125 No Hit GCGAAGAGTTTTTAGTGGAGGACTGATGTAACTTGGGCGTGTTGCATTTG 5 0.125 No Hit ACAGTTTTAGCTGTCGCCATTGTTTTTCTGCTAAAAGAGAGCTCTTTTGA 5 0.125 No Hit GTTATCCTCCTGCATCCATCAAGATTTAGCACTTCAAGAGTCCAACCATG 5 0.125 No Hit GTCCAGATAACTTCCTTAAACCACCATCTGTTACTAAGGTGAATGATAGA 5 0.125 No Hit GCTGGTCCCCAAGCAAACCACTCAATTGCCACCATATATTGTTTGCTTTC 5 0.125 No Hit CTCCCGATCCTCATAAAACACAAAATCATCTAAGATGCATGTTTTGCTGC 5 0.125 No Hit CCTTGACGAAATGGTTGTACTTATCTGACCAATGCGCAGCTGCTGGAACA 5 0.125 No Hit GGATACAGTACTGCTTCGTCGCTACAAACGGAGACACGATCACCCGAGGA 5 0.125 No Hit CCGGCTTCCGGTTGTGTGGTGCTGTCATCTCCTTCTTCAGCTGTGTAAGG 5 0.125 No Hit GGGGGTGCACCAGGAAAGCAGAGCTTGTAATGGACAGTAAGTCCCAGGCA 5 0.125 No Hit CTTCTTAGAGTTTACTGTTGCACGATGCTCGATGCTACGATATGCACCAT 5 0.125 No Hit TCTCATTGCCTTCTCATTGCCGAAGGTCACAAATCCAAAACCACGAGATC 5 0.125 No Hit GGGGAATAGAGCAAGATACGTGCCATGAGAAGGGAGAGAGTGGAGGATGC 5 0.125 No Hit CGTGGCTGTATTGTGCAGGATCAGATGTCAGGACAGCTGATAGCGAAGGG 5 0.125 No Hit GCTCAAGATCCTGTAGTAGCTCTAAAACCTTGCGTCTTTCGGTTGCAAAC 5 0.125 No Hit CCACTACTGAGGAGGGATATCCTCTTATTCCGCTCTCCTAGTAGAGTATT 5 0.125 No Hit ACCAAATTCTCGACTCATTGACTACAACTCCGTGGCTTGTAGTTTAGGCA 5 0.125 No Hit GTATAATTTGGGTTCATACATCGAATTTCTTCTCTTGTTGGAGTGCCAAG 5 0.125 No Hit CTGAGTTCCATCTTGGGAAAGAAGATAATCACGATCTTTCGAGGCCAGAA 5 0.125 No Hit GAGAGAAGCAATTATGCAAGTAGGTCTCGTCCATCCAATGAAGCAAGTCA 5 0.125 No Hit CCTCATTCTTATTTACTTTCCTAGGATGGATTGGAACCACCAGATGCCTT 5 0.125 No Hit GGAGACGGAGGACAAGGTGAAGGGTGGACTCCTTCTGGATATTGTAGTCT 5 0.125 No Hit GCCACAAACAAGATACAGCAATATAAAGTAACTTGAGTTTATTCTCTACT 5 0.125 No Hit CTTGGACTTGAAGCATAATAAAGTCAAATGCCTGTTCTGCCAATGCTACT 5 0.125 No Hit TACCCTTTCTATATGCACATCAATTCTTGATGGCATTGATTCCACGCCAG 5 0.125 No Hit AACTGGCAAAAATGGGTCCTGGAATTGAAGCAAAGATTGCCCCAAATTTT 5 0.125 No Hit CTTTAATAAAGGTTGTTACATTACTTTTACACTGCAGTGGCTCGTCGCTC 5 0.125 No Hit GTCTTGATAAATCACGGGTTTTTGTCGATTTATAGCATTGCTTAGACTAC 5 0.125 No Hit GTACCTTTCTTGTTGTATATGCGACAGAGTACCCAATCATCCAGCCTTAA 5 0.125 No Hit CTACCAGAACAGATGGATTTTCATTCATAGCATGATGATGAGGAACCTGC 5 0.125 No Hit TACCTTTCAATCCCAAGTACTGTTTAGCACGACACTCAAGGCTATCAAAT 5 0.125 No Hit CCTAACCAACTTTGTAACCATCCTCTGCCTCCAGAAAACCCACCAGAAAC 5 0.125 No Hit ATTCAGTCATACAAGCCTTCCTGTTCCCAGACATCAACAAGAAAATCCAC 5 0.125 No Hit GTCGTCGTCATCACTGCGGCGACTCCCATAAGAAGGCCTCTCGTAATCTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0125 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.037500000000000006 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.0625 0.0 0.0 0.0 0.0 68-69 0.0875 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1625 0.0 0.0 0.0 0.0 78-79 0.25 0.0 0.0 0.0 0.0 80-81 0.375 0.0 0.0 0.0 0.0 82-83 0.375 0.0 0.0 0.0 0.0 84-85 0.3875 0.0 0.0 0.0 0.0 86-87 0.475 0.0 0.0 0.0 0.0 88-89 0.6375 0.0 0.0 0.0 0.0 90-91 0.9375 0.0 0.0 0.0 0.0 92-93 1.1 0.0 0.0 0.0 0.0 94-95 1.2625 0.0 0.0 0.0 0.0 96-97 1.4500000000000002 0.0 0.0 0.0 0.0 98-99 1.7125 0.0 0.0 0.0 0.0 100-101 2.0125 0.0 0.0 0.0 0.0 102-103 2.4625 0.0 0.0 0.0 0.0 104-105 2.7625 0.0 0.0 0.0 0.0 106-107 3.4375 0.0 0.0 0.0 0.0 108-109 3.675 0.0 0.0 0.0 0.0 110-111 4.3875 0.0 0.0 0.0 0.0 112-113 4.6875 0.0 0.0 0.0 0.0 114-115 5.0375 0.0 0.0 0.0 0.0 116-117 5.5875 0.0 0.0 0.0 0.0 118-119 6.275 0.0 0.0 0.0 0.0 120-121 6.8375 0.0 0.0 0.0 0.0 122-123 7.574999999999999 0.0 0.0 0.0 0.0 124-125 8.05 0.0 0.0 0.0 0.0 126-127 8.65 0.0 0.0 0.0 0.0 128-129 9.0625 0.0 0.0 0.0 0.0 130-131 9.6375 0.0 0.0 0.0 0.0 132-133 10.4375 0.0 0.0 0.0 0.0 134-135 11.35 0.0 0.0 0.0 0.0 136-137 12.2625 0.0 0.0 0.0 0.0 138-139 13.2375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGAAT 10 0.006830828 145.0 3 AATTTCC 10 0.006830828 145.0 7 CGGAATT 10 0.006830828 145.0 4 TCCGGAA 10 0.006830828 145.0 2 ATTTCCG 10 0.006830828 145.0 8 >>END_MODULE SRR26075406 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075406_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.20325 37.0 37.0 37.0 37.0 37.0 2 36.451 37.0 37.0 37.0 37.0 37.0 3 36.457 37.0 37.0 37.0 37.0 37.0 4 36.4575 37.0 37.0 37.0 37.0 37.0 5 36.3305 37.0 37.0 37.0 37.0 37.0 6 36.3495 37.0 37.0 37.0 37.0 37.0 7 36.306 37.0 37.0 37.0 37.0 37.0 8 36.366 37.0 37.0 37.0 37.0 37.0 9 36.5065 37.0 37.0 37.0 37.0 37.0 10-14 36.37910000000001 37.0 37.0 37.0 37.0 37.0 15-19 36.2849 37.0 37.0 37.0 37.0 37.0 20-24 36.2584 37.0 37.0 37.0 37.0 37.0 25-29 36.1366 37.0 37.0 37.0 37.0 37.0 30-34 36.1289 37.0 37.0 37.0 37.0 37.0 35-39 35.9713 37.0 37.0 37.0 37.0 37.0 40-44 35.981500000000004 37.0 37.0 37.0 37.0 37.0 45-49 35.8594 37.0 37.0 37.0 37.0 37.0 50-54 35.780899999999995 37.0 37.0 37.0 37.0 37.0 55-59 35.851099999999995 37.0 37.0 37.0 37.0 37.0 60-64 35.8658 37.0 37.0 37.0 37.0 37.0 65-69 35.7728 37.0 37.0 37.0 37.0 37.0 70-74 35.6971 37.0 37.0 37.0 37.0 37.0 75-79 35.6514 37.0 37.0 37.0 37.0 37.0 80-84 35.6533 37.0 37.0 37.0 37.0 37.0 85-89 35.6572 37.0 37.0 37.0 37.0 37.0 90-94 35.551100000000005 37.0 37.0 37.0 37.0 37.0 95-99 35.6981 37.0 37.0 37.0 37.0 37.0 100-104 35.4998 37.0 37.0 37.0 37.0 37.0 105-109 35.4753 37.0 37.0 37.0 37.0 37.0 110-114 35.4068 37.0 37.0 37.0 37.0 37.0 115-119 35.386799999999994 37.0 37.0 37.0 37.0 37.0 120-124 35.225350000000006 37.0 37.0 37.0 29.8 37.0 125-129 35.241200000000006 37.0 37.0 37.0 34.6 37.0 130-134 35.30499999999999 37.0 37.0 37.0 34.6 37.0 135-139 35.1801 37.0 37.0 37.0 29.8 37.0 140-144 35.07835 37.0 37.0 37.0 25.0 37.0 145-149 34.99285 37.0 37.0 37.0 25.0 37.0 150-151 34.670874999999995 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 3.0 15 8.0 16 2.0 17 6.0 18 3.0 19 4.0 20 3.0 21 11.0 22 4.0 23 9.0 24 16.0 25 10.0 26 5.0 27 8.0 28 15.0 29 22.0 30 34.0 31 43.0 32 52.0 33 78.0 34 166.0 35 697.0 36 2586.0 37 214.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.43535883970993 19.879969992498125 11.802950737684421 26.881720430107524 2 27.500000000000004 26.450000000000003 28.999999999999996 17.05 3 25.525 25.924999999999997 28.225 20.325 4 27.224999999999998 33.550000000000004 22.05 17.175 5 27.925 35.9 19.5 16.675 6 23.549999999999997 36.925000000000004 21.525 18.0 7 23.575 21.625 37.275000000000006 17.525 8 24.375 26.200000000000003 26.8 22.625 9 24.425 24.625 28.65 22.3 10-14 25.155 29.29 24.32 21.235 15-19 24.665 28.17 26.145000000000003 21.02 20-24 25.069999999999997 27.62 26.450000000000003 20.86 25-29 25.505 28.535 25.635 20.325 30-34 25.03 28.384999999999998 26.284999999999997 20.3 35-39 25.330000000000002 28.735 25.724999999999998 20.21 40-44 24.97 27.445000000000004 27.189999999999998 20.395 45-49 25.69 27.150000000000002 26.064999999999998 21.095 50-54 24.38 28.110000000000003 26.86 20.65 55-59 25.174999999999997 27.955000000000002 26.415 20.455000000000002 60-64 25.655 27.495000000000005 25.915 20.935000000000002 65-69 24.585 27.68 26.810000000000002 20.925 70-74 25.335 28.64 25.924999999999997 20.1 75-79 24.675 28.7 25.895000000000003 20.73 80-84 24.63 27.73 26.76 20.880000000000003 85-89 25.085 28.139999999999997 25.990000000000002 20.785 90-94 24.93 28.38 26.575 20.115 95-99 24.5 28.505000000000003 26.279999999999998 20.715 100-104 25.395 28.475 26.284999999999997 19.845 105-109 24.94 29.185 26.029999999999998 19.845 110-114 25.83 28.444999999999997 25.645 20.080000000000002 115-119 26.6 28.139999999999997 25.295 19.965 120-124 26.451322566128304 28.511425571278565 25.416270813540677 19.620981049052453 125-129 26.424999999999997 28.215 25.650000000000002 19.71 130-134 26.740000000000002 28.189999999999998 26.405 18.665000000000003 135-139 26.022602260226023 27.63776377637764 26.5976597659766 19.741974197419744 140-144 27.359103865579836 28.7993198979847 25.768865329799468 18.072710906635994 145-149 27.731932983245812 27.47186796699175 25.7664416104026 19.02975743935984 150-151 28.028503562945367 27.303412926615827 25.17814726840855 19.489936242030254 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 1.0 16 1.5 17 2.5 18 2.0 19 0.5 20 1.0 21 1.0 22 1.0 23 1.0 24 2.5 25 4.5 26 3.0 27 2.0 28 3.5 29 6.0 30 8.0 31 10.5 32 13.0 33 15.5 34 25.0 35 36.5 36 52.5 37 71.5 38 88.5 39 120.5 40 171.5 41 217.5 42 255.5 43 258.0 44 261.0 45 292.0 46 318.5 47 294.5 48 258.0 49 224.5 50 185.0 51 159.0 52 123.5 53 90.5 54 61.0 55 50.0 56 51.0 57 49.0 58 36.5 59 29.0 60 21.0 61 15.5 62 12.5 63 6.5 64 3.5 65 6.0 66 6.5 67 3.0 68 2.0 69 2.0 70 4.0 71 3.0 72 2.5 73 2.0 74 1.5 75 2.0 76 1.0 77 0.5 78 1.0 79 2.5 80 2.0 81 1.0 82 3.0 83 3.5 84 1.0 85 0.5 86 1.5 87 1.5 88 0.5 89 0.5 90 1.0 91 1.5 92 1.0 93 0.5 94 1.0 95 1.5 96 3.0 97 2.0 98 0.0 99 1.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.01 140-144 0.015 145-149 0.025 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.050000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 63.209144792548685 37.325 2 19.856054191363253 23.45 3 9.271803556308214 16.425 4 4.022015241320914 9.5 5 1.7358171041490262 5.125 6 0.8890770533446233 3.15 7 0.5927180355630821 2.45 8 0.1693480101608806 0.8 9 0.04233700254022015 0.22499999999999998 >10 0.21168501270110077 1.55 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 18 0.44999999999999996 No Hit AAACGATATAGAGGGCACAATTCCCAGCAATTTGACAAACTTAAAGTCTT 14 0.35000000000000003 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 10 0.25 No Hit TACTAGTTGCACAAATACCTCAAACCCTAGTGTCATGCTTGGAGCTGCTT 10 0.25 No Hit CTACATTTCAAATAGGTCTTGTCTAGAATTTCTACTGTAGATCAGAAATG 10 0.25 No Hit GCGGCAAAGTCCAGAGGTTGAGGAAGGAGTGCCCTAATGCTGAGTGCGGT 9 0.22499999999999998 No Hit GAAGTCTGTCAGCATTAACGAGTTCTTGAAGCCTGCTGAAGGGGAGAGGT 8 0.2 No Hit GTCACTTGCTTCTTTTCCTCAAGGATGCTTCTTCTCCACTACAATTGGAG 8 0.2 No Hit CAGTTTCTTTCTTCTCCTCTCTAAAACCAGAGGATAAGTCTGTTCTGTTC 8 0.2 No Hit GGAGGCGGTCCAAAGAAGAAACCGGTGTCCCGCTCCGCCAAAGCCGGTCT 8 0.2 No Hit GTTGTCTACAATTCCTGATGACATTTTATAGAAGTGGATTCATGCTGGCT 7 0.17500000000000002 No Hit TTCAGAGAGCAGTACAAGAGGGAACACCCTAAAAACAAATCCGTTGCAGC 7 0.17500000000000002 No Hit CTCTACTGGCTCAGCTGAAGTTATGTATACCAGAAGAAGTGATGCGTTTT 7 0.17500000000000002 No Hit TGCACTTGACCCGCTGACTACAGTGAAGGCTCGGTTGAACAACTATGGGA 7 0.17500000000000002 No Hit AGGAATGATGCGTTGTGGAACACCAAGAGGAAAAGAAAATGCTGTTGCAG 7 0.17500000000000002 No Hit CTCAACGAAATCTATGATTGGTCACTTACTGGGAGCATCTGGTGCTGTGG 7 0.17500000000000002 No Hit TGAAAGATGAGATACTGGGGTTAATTAAGGGGATTAAAGGGAAATATGGT 7 0.17500000000000002 No Hit CCTGCGCTTCGCTGCACTTAACTCTCAAAAAGAACAGGACGCGGCGTAGC 7 0.17500000000000002 No Hit CATAGGGCTCTCTAAACCACTGGCCGGCAAGGTCGGAGGACCTCCGCCGC 7 0.17500000000000002 No Hit GCAGCTAAGCCCTAATATTCGTAGATCCACTCTCCACGGTACCTCATTCG 7 0.17500000000000002 No Hit AAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGC 7 0.17500000000000002 No Hit ATCAGCAAAAAATGTCTCAAGGCTCTGCCTACCAAACTAGGCAGCGGCAA 7 0.17500000000000002 No Hit GTTCTCGTGACTGGTACAACAAGCAAGGATGACAAGAAAACTTCATGTCT 7 0.17500000000000002 No Hit CAAAAGGTCAAACCTGACAAAAGCAAAACCCAAGAACGTGACCCAAGGAC 7 0.17500000000000002 No Hit GGGAAATGGAGGAACTTTATTTGCAGCAGCAAGAGGAGTTGATGAGAATG 6 0.15 No Hit CTGCCAAGAAATCCCGGGTCAAGTGCTTCATCAAGCTAGTGAACTACCAG 6 0.15 No Hit GAGCAGAAAAGGCTGCCGAGGAGCTCCAAAAATATGATCCTACTAACGAT 6 0.15 No Hit AGAGGGCAGCAAGATGGAGGAAGTCGATTAAGAGGCATAAATTAGCTTTT 6 0.15 No Hit AAAAGATAGATGTCTATAGCTTTGGAGTTGTACTCCTGGAATTGGCGACT 6 0.15 No Hit AAGCTATTCACCTCGATCCTGAGCCATTTGACATGGAGCGTGATCTCATC 6 0.15 No Hit GAAGCACAAAGCAGTACTCCCAGCAAATTTCAAGAAAATTCTAGGTCTTC 6 0.15 No Hit GGATTTACTGCTACATCATTTGGCCGAGAGTGGTCCTAACCGTTACGGGA 6 0.15 No Hit GTTCCAATGATCTTGACACTTGGTCTCACTGGAATTTCATTCTCTGGTGC 6 0.15 No Hit CCCTAACCTCCCTCTCCGATTTCCCTTCAGCAGTCGAAGATGTCGAAGCG 6 0.15 No Hit GGTCGGGCTACTGTTCTCAGGCCAATGTCTTTGATGCAGGCTGTTCGAAG 6 0.15 No Hit GGGTTGTTGTGGGGTCTCAAGTGGAGGCGGTGGACCAAAGGAGGTTCCTG 6 0.15 No Hit GACGCTTTCTCAGCACAAAAAAGCTCCAACGATCTGATCCCACTTCCTCT 6 0.15 No Hit GGCTAGGAAAGCTGCTCTTGAAGAAGAAAGGCGAAAAGAGGAAGAATCTC 6 0.15 No Hit GGTAAGAAGAAGAGTAGTACTCTGGGGAAGTCATCTAGGAAGAAGGAGGA 6 0.15 No Hit GGATCTGAAAAAACTGTCGATGAAGAGTGGTGGAAGAATAAATTCCGGCA 6 0.15 No Hit GCTGATCTTAAGGATCAATTTGATGCCATTGATGTGGATAAAAATGGTGC 6 0.15 No Hit CAAGAAATCAACCTGACATCGCAATCGAATTAACCCAAAAAACCAGACCC 6 0.15 No Hit AGAGGATTGAGACCAGTACCGCCAGAAAAGGGCATCTTCCCCTTGGACCA 6 0.15 No Hit AGGTACTGTATCTCAAGGTACTTTCACGGTTGACTCCAAGAGATATCGCA 6 0.15 No Hit GGAAAATGAGGATTTGAAAAGGAGTGTCCACGCCAAGTGAAATCCAGTCA 6 0.15 No Hit GTTGAAGTCCATGACAGTTGCATCCTGTGTGGGATTGACAGATACTGGAC 5 0.125 No Hit AGAGGAGGAGTTCAAGCTTGAAATTCCAGACAAGGAAGCCGATAAGATTG 5 0.125 No Hit TCGTAATGTCTGCACGGAAGCTGGAATGTCAGCAATCCGTGCAGAACGTG 5 0.125 No Hit GAGATGCTGCACATTCTCCGTGTGGAAGAAGACGAAGCTTGGGAGCCCTT 5 0.125 No Hit CTTCGCAGCAGGTCGCAGCAGGTCGCAGCTTTCGCTTGAATCAAAAGAGC 5 0.125 No Hit CTCAAGGTCAAGTTTTATGTCCCGCCATTCTTGCCCATCATTCCTGTTGT 5 0.125 No Hit GGGCGAGTTGATTTTGACAAGAGTGTCGAGTATTGGCAACAGGACAAGTG 5 0.125 No Hit GAGCCAAGCACACTATTTGATTGAGAAAGCTTCAGAAAAGGCGAAAGTAC 5 0.125 No Hit GGTTGAGAAGCTCTGAGAAAAGTAAAATGACCAGGGACCATAATGGATTC 5 0.125 No Hit CGGAGGTGTTCGAGGTGGCACCGGAGGTTGCGGTGGTGGAGTTCTCCAAG 5 0.125 No Hit CTTGGAGAGTAGTGGAAGGAGAGGAGGAGATTGTCTAATTTATTCAGATC 5 0.125 No Hit GACTCTTCTTTTTTGCTTAGCTTTCTCTACTTGTATAGCCAGACAAGCAC 5 0.125 No Hit GATCGAAGAAGAATCCGAGTCTCAAATTCTTCTGTATAGGAATCTATGGC 5 0.125 No Hit CACGAAAATTGCTTACTACCTTTCATTCCCTGTTTCAGAAACCTCATAAA 5 0.125 No Hit GTAGTTCATCACATAGCTAACTATGGCCACCGAAGACGTTTCCCTCGACC 5 0.125 No Hit CGGCGAGTACAAGCCTCTCCATTCCTCGGTTGATGCGAAACTCCAAGCTA 5 0.125 No Hit GAAGGAGCCAACATGAGGAGGCCAGGGTCCGAATATGAAGAAGGTGGGTC 5 0.125 No Hit TGCCTGAGTGTACTCTCCTACTTTCAGAGAGAGCACAGAGAGAAAAACAA 5 0.125 No Hit CTAATATCTTAGCAATCCAGGCTGGGGTTCTCTCTTGAAAGTTCACGGGG 5 0.125 No Hit ACCAAGTGTTCGCTTTCATTCTCGGATCAATATGACTTGTGTTAACCATG 5 0.125 No Hit GAGCAAGATTGTTTCTGGTGAATATTCAAGTCCACTGGGATTTGCTGCAG 5 0.125 No Hit ATTTGAGTAGGTTGCAGAAAGAAAACGATTTTAAAAATCCTGCTGGTGGG 5 0.125 No Hit GCAGTTGAGTCAGCTGCAAGCTGTCAGGAAGTATCAAAGAGGGAGAAGAA 5 0.125 No Hit CTGGGGATGAGATCAAGGACGCATCAAAACGTGAATCAGGCGATGGGCCG 5 0.125 No Hit TGAGGATGAACTATTATCCACAGTGTCCTCAACCAGAGCAAGTTATTGGC 5 0.125 No Hit GCTCATCGCAGCCCATTGCTGTGCCTATTATTGCTGAAATTGATCTCTAC 5 0.125 No Hit CCGCAACTAACAACCTGTTCGTCTTCTCAGCATTGTAGATGCATGCGGTG 5 0.125 No Hit TGGAGGAATGCAAATCTTTGTTAAGACTTTGACTGGAAAGACCATCACCC 5 0.125 No Hit GGACTGGTCAGTTTGGTGCTCTCCCTGTTATGCCAGTTCAGGCAATGACT 5 0.125 No Hit GAAGAAACTCCCCGCAGAGTCTCATGGCCGTATGGAGTGGAAGACAAATC 5 0.125 No Hit CCAAGGACGGCTCAAGCTTGTAAGAAGATTCCCGTTACAGTAATCACCTC 5 0.125 No Hit CATATCATACATATGTTCCCGATTCTACCGGGCTCCAGAACTTATATTTG 5 0.125 No Hit AGGGGGAGGAAGACTGTGTGGGTGAAGAGGAGAGAGGTCAAGAAGGAGAT 5 0.125 No Hit AAATGGTTACTGGTGAGTATCAGAATCGGGTTTTCTCTGATGATGAAAGA 5 0.125 No Hit GGTATGCAAGTGCGACTCATATGCCACCTTCCGTTCTTAGCCGTGGTGTT 5 0.125 No Hit GTGCTTTGTTGGCGGCCTCGCTTGGGCCACCACTGACCAAGTCCTCCAAG 5 0.125 No Hit AGCTGAAGAGTATAAAAAAGAGTTTTACAGGAAGAGACAGCTGACTCTAG 5 0.125 No Hit GTGATCTTGAAGGCATAAGTACTGATATTGGTGATGGTATTGGTGATGGT 5 0.125 No Hit GGTGATGAAGGCATTGCTAATTTGGCAGGCCTTCCACTTAAAAGTTTGGA 5 0.125 No Hit GTCTCTTCTGTTGTCGCATTTCTTTGCCTGCCGGGGCCATCGTTTACAAC 5 0.125 No Hit TGTTAAGGAAGCAGAGAGTCCTTTCGGTTTCGAAGGAGGTCTACATCGGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0125 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.037500000000000006 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.0625 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.225 0.0 0.0 0.0 0.0 80-81 0.35 0.0 0.0 0.0 0.0 82-83 0.35 0.0 0.0 0.0 0.0 84-85 0.3625 0.0 0.0 0.0 0.0 86-87 0.45 0.0 0.0 0.0 0.0 88-89 0.6125 0.0 0.0 0.0 0.0 90-91 0.9375 0.0 0.0 0.0 0.0 92-93 1.1 0.0 0.0 0.0 0.0 94-95 1.275 0.0 0.0 0.0 0.0 96-97 1.475 0.0 0.0 0.0 0.0 98-99 1.7374999999999998 0.0 0.0 0.0 0.0 100-101 2.075 0.0 0.0 0.0 0.0 102-103 2.4875 0.0 0.0 0.0 0.0 104-105 2.7875 0.0 0.0 0.0 0.0 106-107 3.4625 0.0 0.0 0.0 0.0 108-109 3.675 0.0 0.0 0.0 0.0 110-111 4.4 0.0 0.0 0.0 0.0 112-113 4.7125 0.0 0.0 0.0 0.0 114-115 5.05 0.0 0.0 0.0 0.0 116-117 5.65 0.0 0.0 0.0 0.0 118-119 6.4 0.0 0.0 0.0 0.0 120-121 6.9875 0.0 0.0 0.0 0.0 122-123 7.7875000000000005 0.0 0.0 0.0 0.0 124-125 8.3 0.0 0.0 0.0 0.0 126-127 8.9 0.0 0.0 0.0 0.0 128-129 9.325 0.0 0.0 0.0 0.0 130-131 9.9125 0.0 0.0 0.0 0.0 132-133 10.7 0.0 0.0 0.0 0.0 134-135 11.625 0.0 0.0 0.0 0.0 136-137 12.5625 0.0 0.0 0.0 0.0 138-139 13.6375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCAAAG 10 0.006830828 145.0 145 TTGACCT 10 0.006830828 145.0 8 CTAGTTG 10 0.006830828 145.0 3 ACTAGTT 10 0.006830828 145.0 2 TACTAGT 10 0.006830828 145.0 1 GTTGCAC 10 0.006830828 145.0 6 >>END_MODULE Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035269 spots for SRR26075406.sra Written 2035269 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra Read 2035258 spots for SRR26075406.sra Written 2035258 spots for SRR26075406.sra SRR ids: ['SRR26075406.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_kkxeppen SRR26075406.sra spots: 40705171 blocks: [[1, 2035258], [2035259, 4070516], [4070517, 6105774], [6105775, 8141032], [8141033, 10176290], [10176291, 12211548], [12211549, 14246806], [14246807, 16282064], [16282065, 18317322], [18317323, 20352580], [20352581, 22387838], [22387839, 24423096], [24423097, 26458354], [26458355, 28493612], [28493613, 30528870], [30528871, 32564128], [32564129, 34599386], [34599387, 36634644], [36634645, 38669902], [38669903, 40705171]] SRR26075406 file size 15033583 SRR26075406 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075406 SRR26075406_1.fastq SRR26075406_2.fastq Input file: SRR26075406_1.fastq Paired file: SRR26075406_2.fastq trimmed: SRR26075406-trimmed-pair1.fastq, SRR26075406-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:23:23 2025 >> started Wed Feb 12 01:24:10 2025 >> done (46.708s) 40705171 read pairs processed; of these: 198 ( 0.00%) short read pairs filtered out after trimming by size control 145826 ( 0.36%) empty read pairs filtered out after trimming by size control 40559147 (99.64%) read pairs available; of these: 6696407 (16.51%) trimmed read pairs available after processing 33862740 (83.49%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 17 0.00% 19 17 0.00% 20 22 0.00% 21 11 0.00% 22 30 0.00% 23 29 0.00% 24 22 0.00% 25 32 0.00% 26 25 0.00% 27 25 0.00% 28 45 0.00% 29 36 0.00% 30 27 0.00% 31 30 0.00% 32 54 0.00% 33 37 0.00% 34 60 0.00% 35 54 0.00% 36 62 0.00% 37 47 0.00% 38 57 0.00% 39 55 0.00% 40 96 0.00% 41 86 0.00% 42 96 0.00% 43 109 0.00% 44 80 0.00% 45 101 0.00% 46 132 0.00% 47 139 0.00% 48 172 0.00% 49 170 0.00% 50 173 0.00% 51 220 0.00% 52 237 0.00% 53 303 0.00% 54 290 0.00% 55 350 0.00% 56 396 0.00% 57 423 0.00% 58 450 0.00% 59 552 0.00% 60 688 0.00% 61 821 0.00% 62 964 0.00% 63 1024 0.00% 64 1273 0.00% 65 1284 0.00% 66 1510 0.00% 67 1632 0.00% 68 1881 0.00% 69 2158 0.01% 70 2586 0.01% 71 2800 0.01% 72 3356 0.01% 73 3869 0.01% 74 4438 0.01% 75 5168 0.01% 76 5513 0.01% 77 6474 0.02% 78 7026 0.02% 79 7778 0.02% 80 8728 0.02% 81 9855 0.02% 82 11392 0.03% 83 12968 0.03% 84 14650 0.04% 85 16285 0.04% 86 17495 0.04% 87 19211 0.05% 88 20764 0.05% 89 22025 0.05% 90 24202 0.06% 91 26558 0.07% 92 28594 0.07% 93 32339 0.08% 94 34399 0.08% 95 36575 0.09% 96 39848 0.10% 97 42905 0.11% 98 44800 0.11% 99 46704 0.12% 100 49454 0.12% 101 51711 0.13% 102 54564 0.13% 103 57799 0.14% 104 60183 0.15% 105 64883 0.16% 106 68732 0.17% 107 72118 0.18% 108 73549 0.18% 109 77044 0.19% 110 77329 0.19% 111 79970 0.20% 112 84428 0.21% 113 85810 0.21% 114 90069 0.22% 115 92909 0.23% 116 96495 0.24% 117 99653 0.25% 118 103212 0.25% 119 103977 0.26% 120 106356 0.26% 121 108614 0.27% 122 112046 0.28% 123 113855 0.28% 124 118110 0.29% 125 120073 0.30% 126 124478 0.31% 127 129529 0.32% 128 130883 0.32% 129 133330 0.33% 130 134590 0.33% 131 137231 0.34% 132 140611 0.35% 133 142976 0.35% 134 144529 0.36% 135 149624 0.37% 136 150753 0.37% 137 153991 0.38% 138 156515 0.39% 139 159741 0.39% 140 162468 0.40% 141 164160 0.40% 142 165834 0.41% 143 168360 0.42% 144 170719 0.42% 145 174376 0.43% 146 173880 0.43% 147 176737 0.44% 148 181184 0.45% 149 183163 0.45% 150 185893 0.46% 151 33862740 83.49% 40559147 reads passed initial QC criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=2.92 fanout-score-rank=31 prefix-density=0.42 prefix-fanout=2.5 sequence=ATCTCTGCTACATAATTAGCAGGCCTGTAATACCCAGTAACTGGATCAGGAGCCCATGCAGAGTAGGCCTCAGAATCTTCTTTGGCCACCGCCCCATCTTCCATTTTCCCTGTCATAGCACTGGTCCTTGACCCACCCCTACC criterion=fanout-score sequence-density=0.03 sequence-density-rank=32 fanout-score=183.57 fanout-score-rank=1 prefix-density=0.71 prefix-fanout=9.0 sequence=CCACCACCACCTCCACCACTTCCGCGGGATTGAGCTTCGTTCACGGTGATGTTACGGCCATCGAGGTCCTGACCGTTCATTCCATCAATAGCATCTCTCATTGCCTTCTCATTGCCGAAGGTCACAAATCCAAAACCACGAGATCTTCCGGTTTCACGGTCATTTATAATCTTCGAATCGATGATTTCACCGTACTGGCTAAAAGCCTCTTGGAGGACTTGGTCAGTGGTGGCCCAAGCGAGGCCGCCAACAAAGCACCTGTACTCAACCTCGGCAGACATTGCTAAACCCTAGAAAATTATAGAGAGGAAGAGTAGTGGTCTTTTGGGA criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=2.77 fanout-score-rank=26 prefix-density=0.35 prefix-fanout=2.5 sequence=TGGTTTTACTAG criterion=fanout-score sequence-density=0.04 sequence-density-rank=30 fanout-score=79.68 fanout-score-rank=1 prefix-density=0.51 prefix-fanout=6.3 sequence=AAACCCTAAAAGCTCAAACCTTTGGCGGCTATCCACTGAACCCACCAAAGGGTTTTATTATCTCACAAGATCTGTCATGGCTACCCTTTCATCTTCTGCTGACCAAACTTC SRR26075406 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:24:57 Started mapping on | Feb 12 01:24:57 Finished on | Feb 12 01:33:11 Mapping speed, Million of reads per hour | 295.57 Number of input reads | 40559147 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 35577796 Uniquely mapped reads % | 87.72% Average mapped length | 292.44 Number of splices: Total | 33906433 Number of splices: Annotated (sjdb) | 33035091 Number of splices: GT/AG | 33239249 Number of splices: GC/AG | 532719 Number of splices: AT/AC | 37041 Number of splices: Non-canonical | 97424 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 3.19 Insertion rate per base | 0.02% Insertion average length | 2.47 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 962570 % of reads mapped to multiple loci | 2.37% Number of reads mapped to too many loci | 140715 % of reads mapped to too many loci | 0.35% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 9.23% % of reads unmapped: other | 0.33% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4018781 4018781 4018781 N_multimapping 962570 962570 962570 N_noFeature 971422 35236191 1156025 N_ambiguous 325604 1612 167640 UnstrandedReadsAssigned:34280770 PositiveStrandReadsAssigned:339993 NegativeStrandReadsAssigned:34254131 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075406 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075406-trimmed-pair1.fastq SRR26075406-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 40,559,147 reads, 34,870,583 reads pseudoaligned [quant] estimated average fragment length: 210.528 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,249 rounds 52401 SRR26075406.ke.tsv 34699 SRR26075406.se.tsv 87100 total ==> SRR26075406.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1808.47 3111 48.5876 Potri.005G024800.1.v4.1 1035 825.472 4357 149.081 Potri.004G059700.1.v4.1 961 751.472 3 0.112758 Potri.007G009000.2.v4.1 1416 1206.47 0 0 Potri.003G141000.2.v4.1 2943 2733.47 2143.49 22.1484 Potri.016G087400.1.v4.1 270 90.5777 1977.56 616.661 Potri.015G069301.1.v4.1 564 355.946 0 0 Potri.010G195200.1.v4.1 1773 1563.47 987 17.8305 Potri.012G127500.1.v4.1 977 767.472 39794 1464.51 ==> SRR26075406.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 112 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 333 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 769 SRR26075406 completed mapping pipeline successfully