Starting /dee2/code/volunteer_pipeline.sh SRR26075407 current disk space = 3050810425344 free memory = 1496888508 SRR26075407 SRAfilesize 6a4af1dc13f6d83da0ed26f1552fb5b6 SRR26075407.sra SRR26075407.sra file validated SRR26075407 is paired end SRR26075407 is conventional basespace SRR26075407 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075407_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.65125 37.0 37.0 37.0 37.0 37.0 2 36.4845 37.0 37.0 37.0 37.0 37.0 3 36.588 37.0 37.0 37.0 37.0 37.0 4 36.6395 37.0 37.0 37.0 37.0 37.0 5 36.631 37.0 37.0 37.0 37.0 37.0 6 36.611 37.0 37.0 37.0 37.0 37.0 7 36.651 37.0 37.0 37.0 37.0 37.0 8 36.6135 37.0 37.0 37.0 37.0 37.0 9 36.6595 37.0 37.0 37.0 37.0 37.0 10-14 36.6372 37.0 37.0 37.0 37.0 37.0 15-19 36.581 37.0 37.0 37.0 37.0 37.0 20-24 36.57 37.0 37.0 37.0 37.0 37.0 25-29 36.4751 37.0 37.0 37.0 37.0 37.0 30-34 36.4411 37.0 37.0 37.0 37.0 37.0 35-39 36.348699999999994 37.0 37.0 37.0 37.0 37.0 40-44 36.3312 37.0 37.0 37.0 37.0 37.0 45-49 36.221199999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.2423 37.0 37.0 37.0 37.0 37.0 55-59 36.139300000000006 37.0 37.0 37.0 37.0 37.0 60-64 36.047799999999995 37.0 37.0 37.0 37.0 37.0 65-69 36.0172 37.0 37.0 37.0 37.0 37.0 70-74 36.0558 37.0 37.0 37.0 37.0 37.0 75-79 36.022999999999996 37.0 37.0 37.0 37.0 37.0 80-84 35.958600000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.7911 37.0 37.0 37.0 37.0 37.0 90-94 35.8174 37.0 37.0 37.0 37.0 37.0 95-99 35.86579999999999 37.0 37.0 37.0 37.0 37.0 100-104 35.805699999999995 37.0 37.0 37.0 37.0 37.0 105-109 35.7327 37.0 37.0 37.0 37.0 37.0 110-114 35.521100000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.465799999999994 37.0 37.0 37.0 37.0 37.0 120-124 35.438100000000006 37.0 37.0 37.0 37.0 37.0 125-129 35.293800000000005 37.0 37.0 37.0 29.8 37.0 130-134 35.2738 37.0 37.0 37.0 32.2 37.0 135-139 35.049400000000006 37.0 37.0 37.0 27.4 37.0 140-144 34.85510000000001 37.0 37.0 37.0 25.0 37.0 145-149 34.7615 37.0 37.0 37.0 25.0 37.0 150-151 34.6445 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 1.0 23 5.0 24 2.0 25 8.0 26 15.0 27 20.0 28 19.0 29 27.0 30 27.0 31 40.0 32 73.0 33 119.0 34 188.0 35 484.0 36 2835.0 37 136.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.82352941176471 15.919899874843555 8.585732165206508 36.67083854818523 2 17.575 16.075 34.050000000000004 32.300000000000004 3 16.950000000000003 19.15 29.75 34.150000000000006 4 21.475 24.075 23.724999999999998 30.725 5 22.475 32.4 24.474999999999998 20.65 6 22.125 35.075 23.3 19.5 7 15.875 30.599999999999998 37.925 15.6 8 18.775 29.925 29.675 21.625 9 19.725 26.325 31.574999999999996 22.375 10-14 19.43 31.055 26.674999999999997 22.84 15-19 19.155 28.735 27.950000000000003 24.16 20-24 19.06 29.854999999999997 27.865000000000002 23.22 25-29 19.145 28.655 27.915 24.285 30-34 18.705 29.520000000000003 27.11 24.665 35-39 19.314999999999998 29.404999999999998 27.96 23.32 40-44 19.72 29.015 27.92 23.345 45-49 20.285 28.735 26.775 24.205 50-54 19.12 29.01 28.33 23.54 55-59 19.900000000000002 29.555 26.855 23.69 60-64 19.02 29.604999999999997 27.3 24.075 65-69 20.11 27.639999999999997 28.205000000000002 24.044999999999998 70-74 20.61 28.525 27.37 23.494999999999997 75-79 20.495 28.02 27.27 24.215 80-84 19.88 29.099999999999998 26.240000000000002 24.779999999999998 85-89 19.975 28.305000000000003 27.3 24.42 90-94 20.075000000000003 27.77 26.96 25.195 95-99 19.814999999999998 28.78 27.025 24.38 100-104 20.41 28.555000000000003 27.43 23.605 105-109 21.6 28.544999999999998 26.445 23.41 110-114 21.01 28.310000000000002 27.21 23.47 115-119 21.235 27.92 26.08 24.765 120-124 21.285 27.939999999999998 27.275 23.5 125-129 21.605 27.644999999999996 26.855 23.895 130-134 21.375 27.675 27.834999999999997 23.115 135-139 20.79 27.045 27.095000000000002 25.069999999999997 140-144 21.11 27.810000000000002 26.19 24.89 145-149 21.865000000000002 26.985 26.369999999999997 24.779999999999998 150-151 21.1875 28.1625 26.1 24.55 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.5 18 1.5 19 1.0 20 0.5 21 0.5 22 4.0 23 6.0 24 4.5 25 4.5 26 3.5 27 4.5 28 6.5 29 15.0 30 28.0 31 28.0 32 27.0 33 43.0 34 57.0 35 72.5 36 96.5 37 126.5 38 142.0 39 145.5 40 193.5 41 225.5 42 232.5 43 251.5 44 265.5 45 281.5 46 265.5 47 245.5 48 222.0 49 178.5 50 167.0 51 168.0 52 118.0 53 78.0 54 65.5 55 47.0 56 40.0 57 33.5 58 22.5 59 13.5 60 9.0 61 4.0 62 9.5 63 9.0 64 3.5 65 5.0 66 7.0 67 5.0 68 3.0 69 2.5 70 2.0 71 1.5 72 0.5 73 2.0 74 2.0 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.125 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.9 #Duplication Level Percentage of deduplicated Percentage of total 1 62.684729064039416 38.175 2 22.454844006568145 27.35 3 8.333333333333332 15.225 4 3.32512315270936 8.1 5 1.7651888341543513 5.375 6 0.8620689655172413 3.15 7 0.28735632183908044 1.225 8 0.28735632183908044 1.4000000000000001 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTCCTCGAACCTTGCCTTTGTATCCTTGCAAGCAGTAACTTTCTTATCC 8 0.2 No Hit CAGGAAGCGAAGACTCAAAATTCCCAGGGCGGAAAAATGGAGACTAGAAA 8 0.2 No Hit CGAGTTTTCTTGTCGGAGCCATAACCAATGTTTGGCATCAAAGTGCATCC 8 0.2 No Hit CCTCGATCAACAGCAGGTGGAGCCTCTGTCAAACAAGACATGCCAGAATA 8 0.2 No Hit ATCCAGTAAATTTTCAGTCATTTCCTCTTACCTCATCGGCAATATTTATT 8 0.2 No Hit GAGGGTTACAGTAGGAAGATGGTGGAATTTGATCGAGGGAATGGTGGCAG 8 0.2 No Hit AGTTTACCAAGGAGGATTAGAATTCTCCCTTACAGAAGGTGACCTGGAAC 8 0.2 No Hit AGGGACAAGCGACAAAATCACTACCAAAAAAAGGTTGCAGCCAGAAACAT 7 0.17500000000000002 No Hit GCTCCAATAGGTAATTTTGGGCTTGCCCAAAATATGGCGGTTTTGACATC 7 0.17500000000000002 No Hit TTCACAACATAATAACGAAAGACATGAAAAAAAATATTAACAAAAAAAAA 7 0.17500000000000002 No Hit GCTGGGGGAGATATATATATATATATTGTTGTTCCTTTCTTGTAGCTGGT 7 0.17500000000000002 No Hit CCAGCATATCACAAATCCTGTGTCCCTCAAGAAAGCTGGCCGCAATCAGC 7 0.17500000000000002 No Hit GTACAGGACTAAAGGTAACACAACTGGAGGAGGTTATGCATACAAGAAAG 7 0.17500000000000002 No Hit TATAATGGCCTCTCCTTCTCCAGTGCATGAGATCCCACAAAGATCACCAG 7 0.17500000000000002 No Hit CATCTATTATCATCAATCAACTTGGCAAATGCAGTATAGTGCCCACCACC 6 0.15 No Hit TCCACTGATTAAAGTATATATAAGCAAAATTTAAGAAATTTTACCCATAA 6 0.15 No Hit CTTCAAATCCACATCCAAAGTATACCTAGTAGGCATCAGGTGCTGATAGT 6 0.15 No Hit GACATCTACATCAAGTACTGTAACCATAACCTGATGCCAGGAATCTGAGC 6 0.15 No Hit CTTGAATGAACAAAAGCTACAAAAGCTTCAGATAAAGAGAATGTACAAAT 6 0.15 No Hit GACCAGATAAATACACCCCCAGCATCAACTACATGGTGTTTATTCATAGT 6 0.15 No Hit TGTTGGTGTTTAATCCTCTATACTACTAGAAACAATCTGTTCTCAGTTCG 6 0.15 No Hit GTCCACTAGCTCATTCATCGTGCTTGAGCAGTTGGTGAACATGGCTAAGT 6 0.15 No Hit TTATCATCTTTTTTACAGTTGCTTTGAATTCACCTGAGGCACCCATGTCA 6 0.15 No Hit CTTCATTTGACTTGATGGCCTTCACAGCCTGCTCAGCTGATTTACGGGCA 6 0.15 No Hit ACGGCAGTTGTAGGGTCGCGAGGCCAACACTCAAGTTTCCAATACTATCT 6 0.15 No Hit GTACTGAACTCGTTGCACGAAAGCGCTTAGATATATATTATACAAGTACT 6 0.15 No Hit GAGGAATGCTAAACACAGCATATGGTATGTGATTGTAGTCTGGTTTGGTA 6 0.15 No Hit GGAACAACAACATAAACAGTGAACCTCTCCCCTGCCTCAATCTTGCTAAC 6 0.15 No Hit CTCCTCAGGTGCTGTGGCCACGTCCACTCCAAAGTGGGTTTCGATCAAAC 6 0.15 No Hit CCAGTAACTCCAAACCCACCAAATAAACCTAATTCAAGCTATAGACTAAG 6 0.15 No Hit ATCAAGAAACCAGACAGGACCAACAGCGTTTGCATTCGGCGGCTGCTGCT 6 0.15 No Hit GCGTTATTTGTCCTTTAAATATCACAACCGCTAATTTCTACAGTGTCTAA 6 0.15 No Hit CCTGAATAAATGAAATTGGGTCTGAGTTGCCTATGGTCAACCCTGATACA 6 0.15 No Hit GTACGATTGGATGCATTTGGCTGGTGATAAAATGCATCATTGGTTTCTTT 6 0.15 No Hit ATCTTCCCAAAGGCATACTTGCGAGCCAAATCCATCCTTTGAAGAAATCC 6 0.15 No Hit GTGATAAAGGAGTGGTGGGTGGATGAGGTAGTGGAGGTGATGAGGGGGTA 5 0.125 No Hit GACTGGTTTAGGAGTGGCTCCCTAGTTCTACCTCTAACATTCTCAAAATC 5 0.125 No Hit TCAGTCAGCAATGAGCAAATGGAGAGCAGTACCTTAGAGATGGTAAGAGC 5 0.125 No Hit ACAACCTTTCAAACACTTAAAATTAACACACACGATGATAAATTAACAAT 5 0.125 No Hit CTTCACAATCTTAAGGATCTCCTTGTCAGGAATTTTTCCAGTGCCATAGG 5 0.125 No Hit ACAAAACCAAACCTGGCTAGTTTTTGGGTCACGGGTTGGGCTTGGAGAGT 5 0.125 No Hit GCCCCCAGCAACTTAAGTACATGACCTGCCTCAGTGACTCCTCGGATTGC 5 0.125 No Hit CTCTACTGTGGCCTAACCCTATGGCTCAACACCATTTCCCTAAGTTCAGC 5 0.125 No Hit GAGGGTCTTCACAAAGATCTGCATGCCTCCCCTCAAACGGAGCACCAAGT 5 0.125 No Hit CGGGAGGATTATCGGACATAATTTTGCTCATGACCTTAATTATAAGAGAA 5 0.125 No Hit CCTGGCCAATATTGGAATTTGAATTTTCTCGAGGACAGAATCTTGTTGAA 5 0.125 No Hit TGATAGAGCCCGCAGTCTCCCAGTCACCTGCATGTATATCAACTCCTTGG 5 0.125 No Hit TGCTTGACAATTAGACATGCACCATAACAAGATGCAGATAAAAGTGTCAC 5 0.125 No Hit CTTGTAGCGTCGAGCTGCCTCGTTGCTGTCGATAACTTCTGTTGCAGGAA 5 0.125 No Hit CTTAGATTTCAGTTTTGCACATCTCTGCAGTGTCTCGGAATCAAACCCCA 5 0.125 No Hit CCTGGAAGAAAGAGGTCTGGGATGGGTCAAGCCCTGTGTTGCCAGGAGGG 5 0.125 No Hit CTTCAATACCCAGAGATTTTTTAGGACAATCGTTGACATACATGACAACA 5 0.125 No Hit CTCCTTGTGGGAATTAGGTGGAAACCTAAGGGCACGTACTGCCTCATTAT 5 0.125 No Hit CACCACCATACCCACCACGACGATCATTTGACGATGGTCCTCTACCACCA 5 0.125 No Hit CCAAACAGCAGTGTTCATACTGTACTCTCCATGGGTATGAGGAAAATGCA 5 0.125 No Hit AACACATTTTCATGGTACGGGTCATCATGCCCATGAATTCATTCATGTCT 5 0.125 No Hit GTGGATTTTGATGGTGTACCCATTTGTTTCATGGGGACTGGCTTCGGAGA 5 0.125 No Hit GGAAAAGATTGGTATGAATTCCTCCAGGATGAAGTGAATTAGCAGTTATG 5 0.125 No Hit CTTCAAATGATATCTCGAGTCATCTTCTGCTTGCACACCGACGGGATATA 5 0.125 No Hit CCTTGTTGTTGACGATTGTGGACTCCTCTTTCTTCATCGTCAGGCTTCCT 5 0.125 No Hit TGGCAGTGGGCTTGCTTCCTTCGCCCAGTCGCTCCAACAACTTGAATTCC 5 0.125 No Hit CTGGGTTATGGTCGCCAAATATAATTTGTGCCAGTGCAGTTCCTCCAGCT 5 0.125 No Hit CATCAGCAAATCCCAGTTCGGAGAGCTTCAGATGAGCTTCAGAATAATCG 5 0.125 No Hit CCCTGACATTGATCCACCAGCATCCATTCACCATTAGGCAGAAGATTCTC 5 0.125 No Hit GTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAAC 5 0.125 No Hit GCTCGACACCAATGTGCAATGTTGTTAGCAACAGAGCAAGCCTTCTGCCA 5 0.125 No Hit CCCCTGTGCGTCTCTGACTATTAAGCGTAGGAAGTCTTCAACTGTATGTA 5 0.125 No Hit TATGAGTTTGCCAGTTTAACCTCAGAATGTGTGGGAGTGCTTCCAATGAA 5 0.125 No Hit CCACAGGTTATCGAAACAATACAGCTTTCAGAGAAGTACACATACAAGCA 5 0.125 No Hit ACCTGAAACGCTGCATCTATCACGGATTGGTCAACCAGAGGGTCATCTTG 5 0.125 No Hit GCCTCTAAGACCTGTCTCTGAAGGAGTTCTTCCAGGTGGGGAAGATATTC 5 0.125 No Hit ATCAGCTGTCGTATTGATCTTAGTTTCAGAGTCTCGAAGAGAAGCAGAGT 5 0.125 No Hit CCTTGCTCCTCCAAGATTTTCAGCCACTCCGCCAATTCTTTTGCTGCCTT 5 0.125 No Hit AGTACTCGCAAAAACCTCTTGGAGGAGGGGTTCAGTTACCTGAGTGTGGA 5 0.125 No Hit CATAAGGTTCACCGTAATGAGTCAATGATCTAACATCAGCAACTGGTGCG 5 0.125 No Hit CCACAGTAGCTCCCAATGCTTTAAGTTTTTCGATAATGGGACCAGCCTCT 5 0.125 No Hit ATCAAAACTTCTAGCAACCAAATTGTAGCTCGAGGATACAAGTTTCAGAC 5 0.125 No Hit AGGAAGTTGGTAGGGTAAAGTCAGGGATTCTAGGCTTACAAAGTGGATGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.0625 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.2375 0.0 0.0 0.0 0.0 78-79 0.25 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.35 0.0 0.0 0.0 0.0 84-85 0.4625 0.0 0.0 0.0 0.0 86-87 0.5125 0.0 0.0 0.0 0.0 88-89 0.7124999999999999 0.0 0.0 0.0 0.0 90-91 0.9375 0.0 0.0 0.0 0.0 92-93 0.975 0.0 0.0 0.0 0.0 94-95 1.0625 0.0 0.0 0.0 0.0 96-97 1.3625 0.0 0.0 0.0 0.0 98-99 1.5875 0.0 0.0 0.0 0.0 100-101 1.9625 0.0 0.0 0.0 0.0 102-103 2.3375 0.0 0.0 0.0 0.0 104-105 2.675 0.0 0.0 0.0 0.0 106-107 3.1125 0.0 0.0 0.0 0.0 108-109 3.4375 0.0 0.0 0.0 0.0 110-111 3.9000000000000004 0.0 0.0 0.0 0.0 112-113 4.675000000000001 0.0 0.0 0.0 0.0 114-115 5.225 0.0 0.0 0.0 0.0 116-117 5.6625 0.0 0.0 0.0 0.0 118-119 5.9625 0.0 0.0 0.0 0.0 120-121 6.3375 0.0 0.0 0.0 0.0 122-123 6.875 0.0 0.0 0.0 0.0 124-125 7.4375 0.0 0.0 0.0 0.0 126-127 7.8375 0.0 0.0 0.0 0.0 128-129 8.4 0.0 0.0 0.0 0.0 130-131 9.1625 0.0 0.0 0.0 0.0 132-133 9.8375 0.0 0.0 0.0 0.0 134-135 10.45 0.0 0.0 0.0 0.0 136-137 11.0625 0.0 0.0 0.0 0.0 138-139 11.8625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGACTTG 10 0.006830828 145.0 8 TGTCAAC 10 0.006830828 145.0 5 CAACAAT 10 0.006830828 145.0 8 TCAACAA 10 0.006830828 145.0 7 GCAGTGA 10 0.006830828 145.0 145 GCTAAAC 10 0.006830828 145.0 8 ATGCTAA 10 0.006830828 145.0 6 TTGACTT 10 0.006830828 145.0 7 ATTTGAC 10 0.006830828 145.0 5 TGCTAAA 10 0.006830828 145.0 7 ATGTCAA 10 0.006830828 145.0 4 AATGCTA 10 0.006830828 145.0 5 GGAATGC 10 0.006830828 145.0 3 GACTTGA 10 0.006830828 145.0 9 TGAAAAA 10 0.006830828 145.0 145 CATGTCA 10 0.006830828 145.0 3 >>END_MODULE SRR26075407 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075407_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.34 37.0 37.0 37.0 37.0 37.0 2 36.2165 37.0 37.0 37.0 37.0 37.0 3 36.193 37.0 37.0 37.0 37.0 37.0 4 36.2435 37.0 37.0 37.0 37.0 37.0 5 36.248 37.0 37.0 37.0 37.0 37.0 6 36.1845 37.0 37.0 37.0 37.0 37.0 7 36.2725 37.0 37.0 37.0 37.0 37.0 8 36.2385 37.0 37.0 37.0 37.0 37.0 9 36.227 37.0 37.0 37.0 37.0 37.0 10-14 36.240199999999994 37.0 37.0 37.0 37.0 37.0 15-19 36.1888 37.0 37.0 37.0 37.0 37.0 20-24 36.1773 37.0 37.0 37.0 37.0 37.0 25-29 36.0478 37.0 37.0 37.0 37.0 37.0 30-34 35.970600000000005 37.0 37.0 37.0 37.0 37.0 35-39 35.8476 37.0 37.0 37.0 37.0 37.0 40-44 35.848699999999994 37.0 37.0 37.0 37.0 37.0 45-49 35.7898 37.0 37.0 37.0 37.0 37.0 50-54 35.6537 37.0 37.0 37.0 37.0 37.0 55-59 35.7467 37.0 37.0 37.0 37.0 37.0 60-64 35.7702 37.0 37.0 37.0 37.0 37.0 65-69 35.6968 37.0 37.0 37.0 37.0 37.0 70-74 35.680699999999995 37.0 37.0 37.0 37.0 37.0 75-79 35.5278 37.0 37.0 37.0 37.0 37.0 80-84 35.6112 37.0 37.0 37.0 37.0 37.0 85-89 35.582100000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.4829 37.0 37.0 37.0 37.0 37.0 95-99 35.5673 37.0 37.0 37.0 37.0 37.0 100-104 35.4319 37.0 37.0 37.0 37.0 37.0 105-109 35.4784 37.0 37.0 37.0 37.0 37.0 110-114 35.3762 37.0 37.0 37.0 37.0 37.0 115-119 35.3396 37.0 37.0 37.0 37.0 37.0 120-124 35.238899999999994 37.0 37.0 37.0 37.0 37.0 125-129 35.2544 37.0 37.0 37.0 34.6 37.0 130-134 35.1896 37.0 37.0 37.0 32.2 37.0 135-139 35.0115 37.0 37.0 37.0 27.4 37.0 140-144 35.096799999999995 37.0 37.0 37.0 27.4 37.0 145-149 34.9998 37.0 37.0 37.0 25.0 37.0 150-151 34.70975 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 5.0 15 8.0 16 7.0 17 7.0 18 8.0 19 5.0 20 11.0 21 17.0 22 14.0 23 12.0 24 13.0 25 15.0 26 12.0 27 14.0 28 11.0 29 11.0 30 23.0 31 24.0 32 52.0 33 69.0 34 150.0 35 541.0 36 2720.0 37 248.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.275 21.45 10.825 22.45 2 31.025000000000002 24.7 26.625 17.65 3 25.224999999999998 28.475 28.449999999999996 17.849999999999998 4 26.0 33.675 21.575 18.75 5 27.450000000000003 34.2 21.95 16.400000000000002 6 24.025 36.575 22.85 16.55 7 24.7 22.15 35.725 17.424999999999997 8 21.125 27.3 27.224999999999998 24.349999999999998 9 23.025000000000002 25.45 29.625 21.9 10-14 25.16 29.29 25.419999999999998 20.13 15-19 25.259999999999998 29.2 25.779999999999998 19.759999999999998 20-24 25.505 28.525 26.325 19.645000000000003 25-29 26.224999999999998 27.884999999999998 26.009999999999998 19.88 30-34 25.825 28.544999999999998 26.735 18.895 35-39 24.515 29.270000000000003 26.555 19.66 40-44 25.86 27.595 26.435 20.11 45-49 25.95 27.735 26.195 20.119999999999997 50-54 23.69 29.215000000000003 26.99 20.105 55-59 25.305 29.385 25.705 19.605 60-64 25.945 28.07 26.565 19.42 65-69 25.215 28.139999999999997 26.840000000000003 19.805 70-74 25.285000000000004 29.875 25.485000000000003 19.355 75-79 24.82 28.77 26.715 19.695 80-84 24.38 27.96 27.295 20.365 85-89 25.224999999999998 28.325 26.505000000000003 19.945 90-94 25.21 28.815 27.175 18.8 95-99 25.205 28.275 26.51 20.01 100-104 25.765 28.505000000000003 26.619999999999997 19.11 105-109 25.324999999999996 28.235 26.865 19.575 110-114 25.14 29.075 26.955000000000002 18.83 115-119 24.79 28.315 26.650000000000002 20.244999999999997 120-124 25.135 28.615000000000002 26.6 19.650000000000002 125-129 25.480000000000004 28.09 27.639999999999997 18.790000000000003 130-134 25.895000000000003 28.215 27.015 18.875 135-139 25.97 26.47 27.925 19.634999999999998 140-144 26.384999999999998 28.13 27.455000000000002 18.029999999999998 145-149 26.63 27.855 26.529999999999998 18.985 150-151 26.075 27.55 28.1 18.275 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 1.5 9 2.5 10 2.0 11 2.5 12 4.0 13 3.5 14 3.0 15 2.0 16 1.5 17 1.5 18 1.5 19 1.5 20 1.0 21 1.5 22 2.0 23 1.5 24 4.5 25 4.0 26 2.5 27 4.5 28 5.5 29 9.0 30 9.5 31 8.5 32 9.5 33 14.5 34 31.0 35 51.0 36 59.0 37 64.0 38 91.5 39 134.0 40 180.5 41 225.5 42 246.0 43 279.0 44 313.0 45 292.0 46 288.5 47 282.5 48 260.5 49 221.5 50 179.5 51 153.5 52 115.0 53 84.0 54 73.0 55 63.0 56 34.0 57 22.0 58 25.0 59 21.0 60 11.0 61 10.5 62 10.5 63 7.5 64 3.0 65 2.0 66 2.5 67 2.0 68 1.0 69 2.5 70 3.0 71 0.5 72 1.0 73 1.0 74 0.5 75 1.0 76 1.0 77 1.5 78 1.5 79 1.5 80 1.5 81 1.0 82 1.0 83 0.5 84 0.5 85 0.5 86 0.5 87 2.0 88 2.5 89 3.0 90 2.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 2.0 97 2.0 98 1.0 99 1.5 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.775000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 64.42735734520437 39.800000000000004 2 21.89397005261028 27.05 3 7.567786321327398 14.025000000000002 4 2.954269526507487 7.3 5 1.537838931606637 4.75 6 1.0117361392148927 3.75 7 0.24281667341157423 1.05 8 0.28328611898017 1.4000000000000001 9 0.0 0.0 >10 0.08093889113719142 0.8750000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 23 0.575 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 12 0.3 No Hit GCGAACCAAGGTGCTTAACATCCTTAAAAAGGGACTGCAAGTTGCAGGCA 8 0.2 No Hit CAGTCAGATGATCGAAGATTGTGAGCCATTTGAGGGGGAGGCCCTTTTGG 8 0.2 No Hit GCAACAGTGATTGCTGAAGATGTTTCTGGTATGCCTGGACTTGGTTGTCC 8 0.2 No Hit GTTTTCGAACAGGAGAGGCAGCGCAGCTATCCAAGCAGAAACAATGGCTA 8 0.2 No Hit GTTGTTGGCTGCTCCTGCGTTGTTGTGAAGGATTATGGCGAGACAAGTGA 8 0.2 No Hit ACCCAAGCAAGGTTATAAAGAAAGATTCTACCAAGAAAACAGCAAAGAAA 8 0.2 No Hit CATCAGAAAAGCTTAGACTCATCCGATTTAGGGTTTCGCTTCATATCAAT 8 0.2 No Hit GGTCTCAGTTTTTCATATGTACAGATAAGACTGAATGGCTTGATGGGAAA 7 0.17500000000000002 No Hit GATTTATTGCCACCAAGCAAGATGATTTTGGATGATTGGAAGAGGCTCTA 7 0.17500000000000002 No Hit CAAGAACACAGAGGTATAGGAAAGGGTACGAGCAGCCATGGAGGAGGCCA 7 0.17500000000000002 No Hit CTACAAGATGAAGAGAAAATATTTTTGTACTTTATGTTCCAGGCGGCTGG 7 0.17500000000000002 No Hit CAATGAGTATGAAGTTCAGAGTACTAAACAACCAACAGCATCAGATTCCA 7 0.17500000000000002 No Hit CTCTATGATCGTCCAGTCAAGGAAACCCCAGTGGATGAACCAGCTACAAG 7 0.17500000000000002 No Hit GAAGAGATTAAATTTGGAAGCTGCAGGCGTTGAGCTTGATAAAACAGGAG 6 0.15 No Hit GAACAAGATCATAACTCTTAATGGTTTGGAAGGAGATGTCATGAAAATTT 6 0.15 No Hit GCATTCATTGCAATTGATCGTCGTTGGGAAAAGGATCTTCCATTTGATCC 6 0.15 No Hit GTTCTCATACAGCAGATACCTGAAGAACAAACTGGATACTTTTGTGGATT 6 0.15 No Hit TTTTTGGGGACAACATTAAATGGAATTTTTCCAAGTTCCTGGTAGATAAA 6 0.15 No Hit ATTCTTGCTTGAACTACATCTCTGGCGATGAGCACAAGCTTGATTTAAGC 6 0.15 No Hit AATTCCATTCCAGGAAGATGAAGAAGGGCCATGTGGTACTTTCTATGCCG 6 0.15 No Hit CAGGCATACATAGTGCTATAAAGATGTTGAATAGCAGAATCAGGGTGCTT 6 0.15 No Hit TCTCAACTATTGTCTCACCCTTTTGTATGCAAAAATCCAATATCCGATTT 6 0.15 No Hit TGTGCAGTTATTTAGATCCATTGATGGTGGGGCTGCATTTGGTTTCCCGG 6 0.15 No Hit CAGAAAACAAACAACTCATGATAAAGAAACCAAGACAATCTCTGGAGAAT 6 0.15 No Hit CACAGATTCATCAAGCAAGGGACCTGATGGAGATGTCATTGATGCAGATT 6 0.15 No Hit CTTGAAGGCATAAGTACTGATATTGGTGATGGTATTGGTGATGGTGAAGG 6 0.15 No Hit GACATCTACACCAATTTTGAATCTCCGGATGACTTTGTTCCTCAAGGTCT 6 0.15 No Hit AATAAATCTCTTTCAGCACTTGGTGATGTTATATCTGCCCTCGCATCTAA 6 0.15 No Hit CTGTTGCTGGATTTATATCTGAAGCTATCCAGGGAGTGGGAGGAATTATA 6 0.15 No Hit CATAGTCCTCCAAGGCCGATATGCTGGTCGCAAGGCAGTGATCGTCAAGC 6 0.15 No Hit GCGTGATGTAACTCGCCAGGTTGGTGGGGTTGTGGGGATGGGGAGGTTTC 6 0.15 No Hit GATTTAGTCATTGGTCATTTCCACAATCGTGCACATAATATTTTGGTGGC 6 0.15 No Hit GCAAATTTGTCGAGGATTTAGACTGTTGTAGCTTGCAATGCCTTTTGTTT 6 0.15 No Hit GCCTCTTACAACACTATTTAAAGGGCAGGTTGAAGAGGAGAAAGGTGGAA 6 0.15 No Hit AGAAGGTTTAGTCAAGCCCTCAAGGGTGGAGGCTCCAAAAGGGAGTCCGA 6 0.15 No Hit GGGAGCGGAAAAAATTTCAACGAAAAATGGGAGTGGTGATCATAGATGGA 6 0.15 No Hit GCTGTGGACAGCGAAGGAAGGTGCGCTGCCGCCACTTCAACAGGTGGGCT 6 0.15 No Hit GGATCATGGCAAATTTGGTCAAATACCCAAAAATCCAAGACAGGTTATTC 6 0.15 No Hit GCTAGGTACATAAATAACTTGTCTCTCAAAATAATAGCAACAAAAGAATC 5 0.125 No Hit AAAGAAACCATGCTTCCCCTCCTCCTCCTCTTCACCTCTCTCTTCCTCTC 5 0.125 No Hit AGAAAAATCACGCACATAGACATAGCGGCGCCCTAAATACATGCTATAGT 5 0.125 No Hit AGAGGAAATAACGCAAATTTCCCTTCATACTATGTTGAAACAGCAGAAAA 5 0.125 No Hit CCAAGATCACGAGCTACACATTGGAAACAGACAGTCCTATACCTAGAAGA 5 0.125 No Hit GTGAAGGGAGAGGCTGTTATTAATACACTCCGTAGCCATGGATCCATCTC 5 0.125 No Hit AAACAGCTACTTGTCTTTGTGTGTTGATTAATTGTAGTTAACATGGCAGA 5 0.125 No Hit AAGCAATCGACGTCACAGGGAACAAGGTTAAACAGGCCAAAATCAGAACA 5 0.125 No Hit CGTTTTTCTCGTACAATCTACGTTGGCAACTTACCTGCAGATATAAGAGA 5 0.125 No Hit GCTGATAATGTGGGGTCTACCCAGCTGCAGAATATCAGGAGGGGTCTTAG 5 0.125 No Hit GAACAGATGTGTATTGCTTGGGGTGAGCTCTGGTGCGAACATAGCAGATT 5 0.125 No Hit CCGATCGTGAGTTGAAAGCGTATTGCCTTGCATTTGATGATCAACTTGTT 5 0.125 No Hit CTTTGTATAGAGAGCGAGCCGCTAGCTGCTCTGCAGGACGCTGTTCTTCT 5 0.125 No Hit CTTCGCAACATCAGTATCTCTTATTCTCGTATTTCACTGGCTGATGTAGC 5 0.125 No Hit CTGATGGATCACTTCGTGCCATGAAAGCAAAGATTACACAGTTTATTGCA 5 0.125 No Hit GGCTGGTTCTTCGACGGCAGATATCTATACTCAAAGATAATCCAAAGATT 5 0.125 No Hit TGGAAAAATAAGTGCAGAAGAATTAATGGAGGTCTTGAAGAGGTTGGGAG 5 0.125 No Hit CTTGAGTGGGGAGAAGGAAGGGCTTCTACAACTTCCATCTGACAAGGCTC 5 0.125 No Hit GCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCT 5 0.125 No Hit GAAAGGTGTAACCTTCACGGTAGATTGTACAAAGCCAGTGGAGGACAAGA 5 0.125 No Hit CGATGTTTGCTGACTTAAAAATGGATGATTTTGAATTGGAAAACTCAAGA 5 0.125 No Hit AGTAGAGAAGAGGTACAAGGACCAGTAGTCTTTCCTCACCTATCCCCATT 5 0.125 No Hit CAGCCCTTTTTGTACCCAGACGTTTGCTGCTCCACATCGACGGCCTACCC 5 0.125 No Hit TTCGTAGATCCACTCGCCACGGTACCTCATTCGATCTTTTTGATTCTAGC 5 0.125 No Hit CAATTGCCTATGCTCTCCAACCTCCCATGCTGGTTCTTTCCGGTGCAGGT 5 0.125 No Hit ATTTGTGTTGATATAGAAAACAATGGCACTACATGGAAAGATTGAGACAA 5 0.125 No Hit GGAATTGAAATGCATATGAGTTCTGGTGTTGAAAAGCCAGGGGAGGTTAA 5 0.125 No Hit GGGTTTGCTCCGGTTCATCTCGGTTCCAACGCGTTTAAGCAAGCCAGCAT 5 0.125 No Hit CTGCAAGTGGTTGCGGGGACAATGCATCATCTTACGATTGAAGCGGTTGA 5 0.125 No Hit GTTCTGACACCATCGACAATGTGAAGGCCAAGATCCAGGACAAGGAGGGC 5 0.125 No Hit GCTGAAGGTTGCCTCTGGCTATTGTGAAGGCATTCGCATTCGTGAAGCAA 5 0.125 No Hit GCAAGATCATTATTGACACCTACGGTGGCTGGGGAGCCCATGGTGGTGGT 5 0.125 No Hit GGAGTGGAAGAAGTATCTTGAATACGAGGCAGATGTAATGAAGGATGTTC 5 0.125 No Hit ATAATAATGGCTTCAAAGCGTATTTTGAAGGAACTCAAGGATTTACAGAA 5 0.125 No Hit GTTTCCCCTATGTCTTCAACAGTCAATATTACTGTGAAATAGACAGATAG 5 0.125 No Hit AGTTAGGACTTTTACTGATTTGGGTTTGAAGGAAGCCAAGGACTTGGTGG 5 0.125 No Hit GCTCAGTAAGGATACAGTGACAGTGCCTGCTTTGCCACTCCAACAGCAAT 5 0.125 No Hit CCTGGTATGATCCTTGCCATTTGCATGTTGATCTTAGCCATCATCCTCCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.0625 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.2375 0.0 0.0 0.0 0.0 78-79 0.25 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.35 0.0 0.0 0.0 0.0 84-85 0.4625 0.0 0.0 0.0 0.0 86-87 0.5125 0.0 0.0 0.0 0.0 88-89 0.7124999999999999 0.0 0.0 0.0 0.0 90-91 0.9375 0.0 0.0 0.0 0.0 92-93 0.975 0.0 0.0 0.0 0.0 94-95 1.0625 0.0 0.0 0.0 0.0 96-97 1.3625 0.0 0.0 0.0 0.0 98-99 1.6124999999999998 0.0 0.0 0.0 0.0 100-101 2.0 0.0 0.0 0.0 0.0 102-103 2.3625 0.0 0.0 0.0 0.0 104-105 2.7 0.0 0.0 0.0 0.0 106-107 3.1375 0.0 0.0 0.0 0.0 108-109 3.4625000000000004 0.0 0.0 0.0 0.0 110-111 3.925 0.0 0.0 0.0 0.0 112-113 4.699999999999999 0.0 0.0 0.0 0.0 114-115 5.275 0.0 0.0 0.0 0.0 116-117 5.7125 0.0 0.0 0.0 0.0 118-119 6.0875 0.0 0.0 0.0 0.0 120-121 6.475 0.0 0.0 0.0 0.0 122-123 7.075 0.0 0.0 0.0 0.0 124-125 7.6375 0.0 0.0 0.0 0.0 126-127 8.024999999999999 0.0 0.0 0.0 0.0 128-129 8.575 0.0 0.0 0.0 0.0 130-131 9.3375 0.0 0.0 0.0 0.0 132-133 9.962499999999999 0.0 0.0 0.0 0.0 134-135 10.55 0.0 0.0 0.0 0.0 136-137 11.162500000000001 0.0 0.0 0.0 0.0 138-139 11.975000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCTTAC 10 0.006830828 145.0 3 CAACACT 10 0.006830828 145.0 9 CCTCTTA 10 0.006830828 145.0 2 ACTCAAC 10 0.006830828 145.0 4 CCACTCA 10 0.006830828 145.0 2 TTCCAAG 10 0.006830828 145.0 5 CTTACAA 10 0.006830828 145.0 5 CACTCAA 10 0.006830828 145.0 3 TCCACTC 10 0.006830828 145.0 1 GATTAAA 10 0.006830828 145.0 6 TCTTCCA 10 0.006830828 145.0 3 CCAAGCT 10 0.006830828 145.0 7 AGATTAA 10 0.006830828 145.0 5 TTAAATT 10 0.006830828 145.0 8 GAGATTA 10 0.006830828 145.0 4 CTCAACA 10 0.006830828 145.0 5 >>END_MODULE Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388161 spots for SRR26075407.sra Written 1388161 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra Read 1388148 spots for SRR26075407.sra Written 1388148 spots for SRR26075407.sra SRR ids: ['SRR26075407.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_hxy95qqj SRR26075407.sra spots: 27762973 blocks: [[1, 1388148], [1388149, 2776296], [2776297, 4164444], [4164445, 5552592], [5552593, 6940740], [6940741, 8328888], [8328889, 9717036], [9717037, 11105184], [11105185, 12493332], [12493333, 13881480], [13881481, 15269628], [15269629, 16657776], [16657777, 18045924], [18045925, 19434072], [19434073, 20822220], [20822221, 22210368], [22210369, 23598516], [23598517, 24986664], [24986665, 26374812], [26374813, 27762973]] SRR26075407 file size 10250205 SRR26075407 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075407 SRR26075407_1.fastq SRR26075407_2.fastq Input file: SRR26075407_1.fastq Paired file: SRR26075407_2.fastq trimmed: SRR26075407-trimmed-pair1.fastq, SRR26075407-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:42:03 2025 >> started Wed Feb 12 01:42:34 2025 >> done (31.826s) 27762973 read pairs processed; of these: 141 ( 0.00%) short read pairs filtered out after trimming by size control 102382 ( 0.37%) empty read pairs filtered out after trimming by size control 27660450 (99.63%) read pairs available; of these: 5190167 (18.76%) trimmed read pairs available after processing 22470283 (81.24%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 14 0.00% 19 8 0.00% 20 20 0.00% 21 27 0.00% 22 23 0.00% 23 35 0.00% 24 27 0.00% 25 34 0.00% 26 43 0.00% 27 29 0.00% 28 38 0.00% 29 46 0.00% 30 50 0.00% 31 41 0.00% 32 59 0.00% 33 54 0.00% 34 51 0.00% 35 51 0.00% 36 53 0.00% 37 64 0.00% 38 103 0.00% 39 53 0.00% 40 84 0.00% 41 69 0.00% 42 113 0.00% 43 111 0.00% 44 86 0.00% 45 117 0.00% 46 134 0.00% 47 166 0.00% 48 154 0.00% 49 201 0.00% 50 244 0.00% 51 233 0.00% 52 292 0.00% 53 314 0.00% 54 290 0.00% 55 322 0.00% 56 420 0.00% 57 440 0.00% 58 446 0.00% 59 587 0.00% 60 713 0.00% 61 751 0.00% 62 915 0.00% 63 949 0.00% 64 1088 0.00% 65 1327 0.00% 66 1392 0.01% 67 1576 0.01% 68 1735 0.01% 69 2076 0.01% 70 2505 0.01% 71 2742 0.01% 72 3479 0.01% 73 3908 0.01% 74 4449 0.02% 75 4685 0.02% 76 5333 0.02% 77 5988 0.02% 78 6560 0.02% 79 7649 0.03% 80 8336 0.03% 81 9579 0.03% 82 10418 0.04% 83 12242 0.04% 84 13479 0.05% 85 14798 0.05% 86 15680 0.06% 87 17152 0.06% 88 18185 0.07% 89 19535 0.07% 90 21096 0.08% 91 23316 0.08% 92 25029 0.09% 93 27268 0.10% 94 30232 0.11% 95 31946 0.12% 96 33256 0.12% 97 35569 0.13% 98 37088 0.13% 99 38030 0.14% 100 40713 0.15% 101 42704 0.15% 102 44182 0.16% 103 47094 0.17% 104 49582 0.18% 105 52094 0.19% 106 54586 0.20% 107 56694 0.20% 108 57172 0.21% 109 59362 0.21% 110 60415 0.22% 111 62262 0.23% 112 65802 0.24% 113 67267 0.24% 114 71100 0.26% 115 73530 0.27% 116 74798 0.27% 117 77687 0.28% 118 80201 0.29% 119 81082 0.29% 120 81947 0.30% 121 84565 0.31% 122 86101 0.31% 123 88941 0.32% 124 90947 0.33% 125 93300 0.34% 126 96308 0.35% 127 99354 0.36% 128 100200 0.36% 129 101916 0.37% 130 104053 0.38% 131 104640 0.38% 132 105076 0.38% 133 108839 0.39% 134 110800 0.40% 135 113535 0.41% 136 114519 0.41% 137 116208 0.42% 138 119263 0.43% 139 119994 0.43% 140 120959 0.44% 141 122507 0.44% 142 124420 0.45% 143 125709 0.45% 144 129096 0.47% 145 130362 0.47% 146 132031 0.48% 147 133915 0.48% 148 132890 0.48% 149 135085 0.49% 150 136560 0.49% 151 22470283 81.24% 27660450 reads passed initial QC criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=6.38 fanout-score-rank=20 prefix-density=0.51 prefix-fanout=4.1 sequence=ACCATCACCAATA criterion=fanout-score sequence-density=0.07 sequence-density-rank=32 fanout-score=263.40 fanout-score-rank=1 prefix-density=1.06 prefix-fanout=16.8 sequence=TCATCATCACCACCATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTC criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=2.55 fanout-score-rank=32 prefix-density=0.44 prefix-fanout=2.4 sequence=TATTGGTGATGGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=159.58 fanout-score-rank=1 prefix-density=0.15 prefix-fanout=10.0 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGGCAAGCTTAGAAAGATCAGCACCAGACCACTTAACATCAAAGATATTGACGTCGGAGAGGGGAGCAGTCCTGTTAATAAGTTTCAGAGGTCCATGACTATGCCAGGAACTCCAGGGACACCGACGACACCAGTGACCCCTACAACCCCAGTGTCGGCGCGTAGCAATGTTTGGAGGAGCGTGTTCCACCCTGGTAGCAACCTTGCTACTAAGAATATTGGTGCTCATGTTTTTGACAAGCCACAGCCTAACACACCCACTGTCTATGACTGGATGTACAGTGGAGAGACGAAGAGCGAGCATCGTTGATGAGGTTGCCTTCAACCAAGGTTGCCCATGTAAATACGTACTGTGTTTTGTTTTTCAGTACTCATCTGCAATATGTCTCTTGTTGTTATGGTTCTACGGTTCTACCGTGCCTGGAA SRR26075407 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:43:16 Started mapping on | Feb 12 01:43:16 Finished on | Feb 12 01:46:40 Mapping speed, Million of reads per hour | 488.13 Number of input reads | 27660450 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 24920293 Uniquely mapped reads % | 90.09% Average mapped length | 290.66 Number of splices: Total | 20928206 Number of splices: Annotated (sjdb) | 20350087 Number of splices: GT/AG | 20518628 Number of splices: GC/AG | 306635 Number of splices: AT/AC | 26174 Number of splices: Non-canonical | 76769 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.05% Deletion average length | 4.16 Insertion rate per base | 0.02% Insertion average length | 2.59 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 906072 % of reads mapped to multiple loci | 3.28% Number of reads mapped to too many loci | 114594 % of reads mapped to too many loci | 0.41% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.76% % of reads unmapped: other | 0.45% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1834085 1834085 1834085 N_multimapping 906072 906072 906072 N_noFeature 692772 24595458 846921 N_ambiguous 321843 1705 150296 UnstrandedReadsAssigned:23905678 PositiveStrandReadsAssigned:323130 NegativeStrandReadsAssigned:23923076 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075407 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075407-trimmed-pair1.fastq SRR26075407-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 27,660,450 reads, 24,609,315 reads pseudoaligned [quant] estimated average fragment length: 203.996 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,331 rounds 52401 SRR26075407.ke.tsv 34699 SRR26075407.se.tsv 87100 total ==> SRR26075407.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1815 2784 46.468 Potri.005G024800.1.v4.1 1035 832.004 4732 172.299 Potri.004G059700.1.v4.1 961 758.02 32 1.27888 Potri.007G009000.2.v4.1 1416 1213 0 0 Potri.003G141000.2.v4.1 2943 2740 1134 12.5379 Potri.016G087400.1.v4.1 270 93.644 4704 1521.77 Potri.015G069301.1.v4.1 564 362.399 0 0 Potri.010G195200.1.v4.1 1773 1570 538 10.3811 Potri.012G127500.1.v4.1 977 774.009 6988 273.507 ==> SRR26075407.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 571 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 622 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 123 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 558 SRR26075407 completed mapping pipeline successfully