Starting /dee2/code/volunteer_pipeline.sh SRR26075408 current disk space = 3050810212352 free memory = 1563689644 SRR26075408 SRAfilesize c332659d25692351c119aa73a9ba8af6 SRR26075408.sra SRR26075408.sra file validated SRR26075408 is paired end SRR26075408 is conventional basespace SRR26075408 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075408_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6385 37.0 37.0 37.0 37.0 37.0 2 36.6485 37.0 37.0 37.0 37.0 37.0 3 36.7175 37.0 37.0 37.0 37.0 37.0 4 36.712 37.0 37.0 37.0 37.0 37.0 5 36.7045 37.0 37.0 37.0 37.0 37.0 6 36.6565 37.0 37.0 37.0 37.0 37.0 7 36.572 37.0 37.0 37.0 37.0 37.0 8 36.739 37.0 37.0 37.0 37.0 37.0 9 36.7305 37.0 37.0 37.0 37.0 37.0 10-14 36.6537 37.0 37.0 37.0 37.0 37.0 15-19 36.6318 37.0 37.0 37.0 37.0 37.0 20-24 36.5858 37.0 37.0 37.0 37.0 37.0 25-29 36.5062 37.0 37.0 37.0 37.0 37.0 30-34 36.471999999999994 37.0 37.0 37.0 37.0 37.0 35-39 36.4358 37.0 37.0 37.0 37.0 37.0 40-44 36.3279 37.0 37.0 37.0 37.0 37.0 45-49 36.2243 37.0 37.0 37.0 37.0 37.0 50-54 36.2121 37.0 37.0 37.0 37.0 37.0 55-59 36.01649999999999 37.0 37.0 37.0 37.0 37.0 60-64 35.964 37.0 37.0 37.0 37.0 37.0 65-69 35.8938 37.0 37.0 37.0 37.0 37.0 70-74 36.058299999999996 37.0 37.0 37.0 37.0 37.0 75-79 36.12820000000001 37.0 37.0 37.0 37.0 37.0 80-84 36.1216 37.0 37.0 37.0 37.0 37.0 85-89 36.01090000000001 37.0 37.0 37.0 37.0 37.0 90-94 35.992599999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.96569999999999 37.0 37.0 37.0 37.0 37.0 100-104 35.9553 37.0 37.0 37.0 37.0 37.0 105-109 35.8241 37.0 37.0 37.0 37.0 37.0 110-114 35.724199999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.6069 37.0 37.0 37.0 37.0 37.0 120-124 35.623200000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.3962 37.0 37.0 37.0 34.6 37.0 130-134 35.2898 37.0 37.0 37.0 34.6 37.0 135-139 35.223400000000005 37.0 37.0 37.0 32.2 37.0 140-144 35.0139 37.0 37.0 37.0 25.0 37.0 145-149 34.8959 37.0 37.0 37.0 25.0 37.0 150-151 34.8505 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 2.0 22 3.0 23 1.0 24 1.0 25 4.0 26 10.0 27 14.0 28 14.0 29 11.0 30 35.0 31 53.0 32 77.0 33 126.0 34 180.0 35 445.0 36 2837.0 37 186.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 48.14629258517034 12.975951903807614 5.410821643286573 33.46693386773547 2 19.475 15.174999999999999 33.15 32.2 3 18.075 19.900000000000002 31.374999999999996 30.65 4 21.8 26.525 25.5 26.174999999999997 5 22.3 33.6 23.425 20.674999999999997 6 19.575 37.45 23.075000000000003 19.900000000000002 7 16.525000000000002 28.599999999999998 38.175 16.7 8 18.224999999999998 27.875 30.425 23.474999999999998 9 19.85 22.8 33.425 23.925 10-14 20.23202320232023 29.662966296629662 26.527652765276528 23.577357735773578 15-19 20.125 27.66 27.700000000000003 24.515 20-24 19.564999999999998 27.715 28.395 24.325 25-29 19.495 28.970000000000002 26.25 25.285000000000004 30-34 19.72 28.285 27.889999999999997 24.104999999999997 35-39 19.715 28.675 27.32 24.29 40-44 20.119999999999997 27.16 27.36 25.36 45-49 20.205000000000002 27.58 27.92 24.295 50-54 20.27 27.925 27.665 24.14 55-59 20.685000000000002 28.425 27.644999999999996 23.244999999999997 60-64 20.41 27.925 27.52 24.145 65-69 20.285 27.565 28.360000000000003 23.79 70-74 21.675 26.895000000000003 27.810000000000002 23.62 75-79 21.815 28.000000000000004 26.834999999999997 23.35 80-84 20.785 27.439999999999998 26.99 24.785 85-89 22.15 27.01 26.815 24.025 90-94 22.155 27.16 26.669999999999998 24.015 95-99 21.759999999999998 27.88 26.955000000000002 23.405 100-104 22.585 27.935 25.81 23.669999999999998 105-109 22.445 27.11 26.55 23.895 110-114 21.765 26.655 27.865000000000002 23.715 115-119 22.405 26.71 27.24 23.645 120-124 21.41 27.065 27.045 24.48 125-129 22.535 27.18 26.575 23.71 130-134 22.14 27.944999999999997 25.69 24.224999999999998 135-139 22.125 27.675 25.7 24.5 140-144 22.395 27.295 26.174999999999997 24.135 145-149 22.89 27.715 25.0 24.395 150-151 24.1125 26.6125 25.424999999999997 23.849999999999998 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.5 17 0.5 18 0.5 19 0.5 20 0.5 21 1.0 22 1.5 23 1.5 24 2.5 25 2.5 26 0.5 27 5.0 28 11.5 29 13.5 30 18.0 31 21.0 32 23.0 33 37.0 34 49.0 35 58.5 36 75.0 37 98.5 38 110.0 39 131.5 40 144.5 41 167.0 42 241.5 43 271.5 44 270.5 45 293.5 46 294.5 47 260.0 48 224.5 49 195.0 50 182.5 51 150.0 52 112.5 53 93.5 54 82.0 55 69.5 56 53.0 57 45.5 58 37.5 59 29.0 60 16.5 61 14.0 62 12.5 63 7.5 64 6.5 65 9.5 66 11.5 67 9.5 68 9.0 69 10.0 70 5.5 71 2.0 72 2.0 73 1.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.675000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 62.91718170580965 38.175 2 21.714050267820355 26.35 3 8.941079522043676 16.275000000000002 4 3.584672435105068 8.7 5 1.8541409147095178 5.625 6 0.3708281829419036 1.35 7 0.3296250515039143 1.4000000000000001 8 0.08240626287597858 0.4 9 0.08240626287597858 0.44999999999999996 >10 0.12360939431396785 1.275 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGGACTTATCTCGTAT 30 0.75 TruSeq Adapter, Index 20 (97% over 37bp) GTCAATGAACTATGTATTCTGGTTTCTTCTGACGAACCAAAGAATTATAT 11 0.27499999999999997 No Hit GTCGTCCTTCAGATAATCAGAAGTTTCCAGGGTTGTCCTTCGGAAGAAAC 10 0.25 No Hit GCACCACTTCTATCCACCTTAGTTGGGTCCTTCCCTGAGAAAGCACCACC 9 0.22499999999999998 No Hit ATCCGAACCTGGCTGTGGATGATGGACAAACAAAAACGGACACCGAATTT 9 0.22499999999999998 No Hit GATACTACAAGCTTGTCTTTGCCAACAATGCCCTCGAGAGCTTTTAGTGT 8 0.2 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGGACTTATCGCGTAT 8 0.2 TruSeq Adapter, Index 20 (97% over 37bp) GTTAATTGGTGACTGGATGAAGATGGAGAAGGTTGTGGGCTTGGGAGAGT 7 0.17500000000000002 No Hit CCAGTTCCAAGGCAACGGGAGCAGTAGCTGAGGCCACTGCCACCACAAGT 7 0.17500000000000002 No Hit ACAACACACCAATCACCAGCACACTATCATATACCATTACATACAACTGA 7 0.17500000000000002 No Hit CCTCAATTCCAGAAACGGACTGGGTATCATTTTCTACTGCTTGAGTTGGA 7 0.17500000000000002 No Hit CCCACAAACCCTATCAGATTTTCACCAGCCATGCTACTAGGCTAAGGATG 7 0.17500000000000002 No Hit CCCGATTCGATGCACAGCAGTAACTGGGAAACTTGAAGTCCCATGATCAC 7 0.17500000000000002 No Hit CCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCTCTATCCTACCAT 7 0.17500000000000002 No Hit GGTCATTAAGAGATGCAAATCCTGAAAAGATTGCTGTCTGCTTCAACTCT 7 0.17500000000000002 No Hit AGGGTGCACAAAACATAAAACTTAAATTCCTTGGCAAATAAAAAGCATAC 6 0.15 No Hit CAATAAGACCATCACCACCTAGCTTGCTGGCCATAAGAGGCAAGAAATCT 6 0.15 No Hit CTCTTCTAAATTCACTAGGATCTATACGAAAAACAACCTCTCAGTTGCAC 6 0.15 No Hit GGGCAGATCAATTTTCCATGCAATACCAGAAAGAAATGCGTACCAACCAG 6 0.15 No Hit GTAGAAGGACGTTCGGGTCGGGTAATCGGGCCAAATATTGTCAGTCTCAA 6 0.15 No Hit CTGGAAACTTTCCCACCGTTGTAGCTCATAAGCCTTCTGGCACCTGCCAT 6 0.15 No Hit GTTGATATGCATAATTTCTTGTCAACTCAGCTAATCTCAACCCCCAACAT 6 0.15 No Hit CCTTACTTGGCGCCACCACTTCTTTCGAATGCTTTGAGAGCTTGATCTAA 6 0.15 No Hit GTCTCCAACAAGTAATATGTCATTTTCATGATCAACATAAACCAGTTTCC 6 0.15 No Hit GCTATTTCAATTTCCCCTAGAGCTTCAACGCATTTTATTAAAACCAAAGT 5 0.125 No Hit CTCTGAAAAGGGAAGGAAAGGATCATAAGAAAATTCTCTAGAACTATAAC 5 0.125 No Hit GGAATAATCATGAGGAGGCTTCTGCGTACACATGTTGTAAATGGTTGTGT 5 0.125 No Hit CCTTGATCTTCTCCATCATTCCCTTTTTCACCACAGGCTCTCCTTTATGA 5 0.125 No Hit ACTGTATAGCCCTTCTGGTTCATCTCCTTGACGAAATGGTTGTACTTATC 5 0.125 No Hit GGTTGATGTTTCGCCAGAACCATTGTTACTTCCCTCGTCCATCTTTCTCT 5 0.125 No Hit CTTCTATCCATGCCAAGCGATCCTCACGTGTCTCTGCTCTCAAGTGCAGC 5 0.125 No Hit GCTTCTTCAAGTTTCCTCAGCTCAAGTTCAACACCCTCTATCTTCTCTTT 5 0.125 No Hit GTTACCATTCACTTCAACCTTATCAAATATGGGATATTCAGCCTTAAAGC 5 0.125 No Hit GGGTACTGTATGCTGAGCATAGTTCGGAAGATCCTGATCCAAATTTGAAA 5 0.125 No Hit GGGTCTTGACAAAGATCTGCATACCACCACGGAGGCGAAGCACCAAATGA 5 0.125 No Hit GCCTCCTCCTCACTCTGGCCACCAGACAAGAATACAATAGCAGGAACTGC 5 0.125 No Hit GGGAAAATAAATAAAAAAAACTAATAAAAAAAACTGTTACCCTACAACAA 5 0.125 No Hit CTGCTAGTGACAATTCACTCATGCCCTCACCATAGAACTCCACAAACTTG 5 0.125 No Hit CACATTCTCCAGTAAATACACCCGAATTATATAACTGGAAATTTCTGCCA 5 0.125 No Hit GCGGAAGTATAACTTGACCCCCGAAACTTCCAACTCGAGAACCTTGTAGG 5 0.125 No Hit GGCAGATCCTTCCTTCCTTTCCCTTTTCACTTCCAGTATCTTCATCTTCC 5 0.125 No Hit CCCACTTTCATTCTGGGAGCTGCAAAGGAGTTGGAGGTCTACTACAGCTG 5 0.125 No Hit GTGGGAAATCACAAATATCTTCTGCTTTGAGGATATAACTAGATACAAAG 5 0.125 No Hit GCGAAACATCAACCCTGTGAGCAGCTTGAACAAGACAAGCAACCTTATGG 5 0.125 No Hit CTCATTTGCAGGAGCATGGATCACAGGTGCAGTTTGATCCACACTTGCAG 5 0.125 No Hit GGCATTGGTTGACCTTGTTGAGGTGGCTGGCCATGCCAAGCTGATTGACC 5 0.125 No Hit GCAATTGAGAAAGCTCTTTAATAGACGTTACAGACCAAGCATCCTAGTCT 5 0.125 No Hit TTTTTTTTTTTCAAATAAAGCCACACTAGTATTCATTATTGATGTCTGTG 5 0.125 No Hit GGGAAAAAGAAACTAGCCACCTGTCATCAAAAAATGGAGTTTCTGGGTCT 5 0.125 No Hit CAAATGGAGACATAGCTAAAGCATCATAATGGAGTCCATCGTAAATCAGC 5 0.125 No Hit GTTGTCTTACCAGCAGCATCAAGACCCACCATCAGAATACGCATTTCCTT 5 0.125 No Hit GGCCAATGCATCCCTGAACCCTTAGTAAATCCTATATCTTTACACCCATC 5 0.125 No Hit GTGAGATAGCATTCTCATCATCACAAATTATTCCTTCCTATCCATCATCA 5 0.125 No Hit GTATTTGTGTGTGATGAGGATGAGAAGGTGGTAGCAAGGCAAACAGCACC 5 0.125 No Hit TTCGGATCTTCTTTCTTCTTTTCAGGCTCTTTTGCTGGACCTACTGAGAT 5 0.125 No Hit GATTTCTGAAACTCCAAAGAACGAGCCCTCTGTATCTTTGTTAATGGAGT 5 0.125 No Hit CTCTAGCACAGTCCACAAGCTGCTGTTCAGACAGAGAGATTCCTTTCCCA 5 0.125 No Hit GCCGGCGAGTACATCCCGTGAAACTCGCTTGAAGGAGTGGTTTCTCTACT 5 0.125 No Hit GTGCTTGAGGCCTCTGTTTGATTTCTCCTCAGCTCCTCGGCTTCTATCAG 5 0.125 No Hit AACACTAACAAATAAAGGCTTAAAAATAAACAAATAATACAATTTTCAAT 5 0.125 No Hit CCTGTTTCTTGAGCTGCTGTTGCTTAGCCTTTTTCTCCTGGATCATCTTC 5 0.125 No Hit GTTCAGAATAAATTTTACATTCTCGTGCTGCTGTAGCACCGTCAAAATAT 5 0.125 No Hit ACCACCCTTTTCCACATCTGTAAGATACATGAGTACAGTTGCCACCCGGT 5 0.125 No Hit TACTGCAAGAACAAGGAGTGCAAAAAGCACACCTTGCACAAGGTCACTCA 5 0.125 No Hit TCACAATAGCATTTTCAAAATCATAGTCATCATTTTCATGGACATCTTTG 5 0.125 No Hit ACGACCACCAATTGATCTCCAAGGGCGCGTGCTTGGCGGAGGGCGTTGCA 5 0.125 No Hit GTCCAACAACCTGAAAGCTCAGACCTTATCCGGTGATTAGGGTTTCGACG 5 0.125 No Hit TGGTCCTTTAAAACTATGGCTCTGTGACAAACAAACCCTAGCCTCAGCAG 5 0.125 No Hit GCATGATTTTCTTCTATCTATATGACGCTAAGTCAGGAAATACACCAACT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.075 0.0 0.0 0.0 0.0 48-49 0.075 0.0 0.0 0.0 0.0 50-51 0.075 0.0 0.0 0.0 0.0 52-53 0.075 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.0875 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.1375 0.0 0.0 0.0 0.0 62-63 0.15 0.0 0.0 0.0 0.0 64-65 0.175 0.0 0.0 0.0 0.0 66-67 0.2 0.0 0.0 0.0 0.0 68-69 0.21250000000000002 0.0 0.0 0.0 0.0 70-71 0.30000000000000004 0.0 0.0 0.0 0.0 72-73 0.35 0.0 0.0 0.0 0.0 74-75 0.3625 0.0 0.0 0.0 0.0 76-77 0.4125 0.0 0.0 0.0 0.0 78-79 0.425 0.0 0.0 0.0 0.0 80-81 0.575 0.0 0.0 0.0 0.0 82-83 0.675 0.0 0.0 0.0 0.0 84-85 0.7250000000000001 0.0 0.0 0.0 0.0 86-87 0.775 0.0 0.0 0.0 0.0 88-89 0.9375 0.0 0.0 0.0 0.0 90-91 1.2625000000000002 0.0 0.0 0.0 0.0 92-93 1.4874999999999998 0.0 0.0 0.0 0.0 94-95 1.9 0.0 0.0 0.0 0.0 96-97 2.25 0.0 0.0 0.0 0.0 98-99 2.5875000000000004 0.0 0.0 0.0 0.0 100-101 2.9125 0.0 0.0 0.0 0.0 102-103 3.3125 0.0 0.0 0.0 0.0 104-105 3.9250000000000003 0.0 0.0 0.0 0.0 106-107 4.0875 0.0 0.0 0.0 0.0 108-109 4.449999999999999 0.0 0.0 0.0 0.0 110-111 5.0375 0.0 0.0 0.0 0.0 112-113 5.550000000000001 0.0 0.0 0.0 0.0 114-115 5.824999999999999 0.0 0.0 0.0 0.0 116-117 6.362500000000001 0.0 0.0 0.0 0.0 118-119 6.9625 0.0 0.0 0.0 0.0 120-121 7.6 0.0 0.0 0.0 0.0 122-123 8.1875 0.0 0.0 0.0 0.0 124-125 8.825 0.0 0.0 0.0 0.0 126-127 9.787500000000001 0.0 0.0 0.0 0.0 128-129 10.537500000000001 0.0 0.0 0.0 0.0 130-131 10.9 0.0 0.0 0.0 0.0 132-133 11.3625 0.0 0.0 0.0 0.0 134-135 12.1875 0.0 0.0 0.0 0.0 136-137 12.9875 0.0 0.0 0.0 0.0 138-139 13.9 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACTTG 10 0.006830828 145.0 3 GGCGCCA 10 0.006830828 145.0 9 CCTTACT 10 0.006830828 145.0 1 TGGCGCC 10 0.006830828 145.0 8 TTTTTTT 20 0.00593511 29.0 15-19 >>END_MODULE SRR26075408 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075408_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.18625 37.0 37.0 37.0 37.0 37.0 2 36.2685 37.0 37.0 37.0 37.0 37.0 3 36.2405 37.0 37.0 37.0 37.0 37.0 4 36.259 37.0 37.0 37.0 37.0 37.0 5 36.286 37.0 37.0 37.0 37.0 37.0 6 36.2275 37.0 37.0 37.0 37.0 37.0 7 36.257 37.0 37.0 37.0 37.0 37.0 8 36.238 37.0 37.0 37.0 37.0 37.0 9 36.2965 37.0 37.0 37.0 37.0 37.0 10-14 36.1528 37.0 37.0 37.0 37.0 37.0 15-19 36.0167 37.0 37.0 37.0 37.0 37.0 20-24 35.9477 37.0 37.0 37.0 37.0 37.0 25-29 35.7579 37.0 37.0 37.0 37.0 37.0 30-34 35.6248 37.0 37.0 37.0 37.0 37.0 35-39 35.6059 37.0 37.0 37.0 37.0 37.0 40-44 35.5277 37.0 37.0 37.0 37.0 37.0 45-49 35.459999999999994 37.0 37.0 37.0 37.0 37.0 50-54 35.334 37.0 37.0 37.0 37.0 37.0 55-59 35.4413 37.0 37.0 37.0 37.0 37.0 60-64 35.4358 37.0 37.0 37.0 37.0 37.0 65-69 35.3292 37.0 37.0 37.0 37.0 37.0 70-74 35.29 37.0 37.0 37.0 37.0 37.0 75-79 35.2303 37.0 37.0 37.0 37.0 37.0 80-84 35.237199999999994 37.0 37.0 37.0 37.0 37.0 85-89 35.239 37.0 37.0 37.0 37.0 37.0 90-94 35.22959999999999 37.0 37.0 37.0 37.0 37.0 95-99 35.32340000000001 37.0 37.0 37.0 37.0 37.0 100-104 35.2318 37.0 37.0 37.0 34.6 37.0 105-109 35.1896 37.0 37.0 37.0 32.2 37.0 110-114 35.1103 37.0 37.0 37.0 32.2 37.0 115-119 35.1034 37.0 37.0 37.0 32.2 37.0 120-124 35.041399999999996 37.0 37.0 37.0 27.4 37.0 125-129 34.986599999999996 37.0 37.0 37.0 27.4 37.0 130-134 34.9624 37.0 37.0 37.0 25.0 37.0 135-139 34.77285 37.0 37.0 37.0 25.0 37.0 140-144 34.7803 37.0 37.0 37.0 25.0 37.0 145-149 34.744550000000004 37.0 37.0 37.0 25.0 37.0 150-151 34.506625 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 5.0 14 8.0 15 17.0 16 11.0 17 11.0 18 7.0 19 10.0 20 6.0 21 10.0 22 20.0 23 22.0 24 16.0 25 18.0 26 26.0 27 20.0 28 19.0 29 19.0 30 21.0 31 26.0 32 46.0 33 79.0 34 165.0 35 547.0 36 2648.0 37 222.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.562140535133786 23.78094523630908 8.027006751687923 19.629907476869217 2 30.55 26.575 23.799999999999997 19.075 3 23.400000000000002 26.924999999999997 32.05 17.625 4 27.35 31.974999999999998 22.425 18.25 5 27.800000000000004 35.725 18.875 17.599999999999998 6 24.95 36.325 20.325 18.4 7 24.7 23.799999999999997 32.475 19.025 8 21.625 26.150000000000002 25.6 26.625 9 24.525 23.674999999999997 28.15 23.65 10-14 26.5 29.38 24.310000000000002 19.81 15-19 26.040000000000003 27.455000000000002 25.95 20.555 20-24 26.055 28.025 25.575 20.345 25-29 26.995 28.525 24.990000000000002 19.49 30-34 26.029999999999998 27.33 25.759999999999998 20.880000000000003 35-39 25.77 27.655 25.869999999999997 20.705000000000002 40-44 25.71 28.360000000000003 25.605 20.325 45-49 25.8 27.994999999999997 25.885 20.32 50-54 24.4 28.21 27.82 19.57 55-59 26.119999999999997 28.49 25.28 20.11 60-64 26.38 27.565 26.36 19.695 65-69 25.845000000000002 28.199999999999996 26.655 19.3 70-74 24.805 28.084999999999997 26.845000000000002 20.265 75-79 24.48 29.165000000000003 25.89 20.465 80-84 25.205 28.595 26.135 20.064999999999998 85-89 24.705 27.825 26.16 21.310000000000002 90-94 26.279999999999998 28.93 25.585 19.205 95-99 25.919999999999998 28.46 25.415 20.205000000000002 100-104 25.575 29.125 26.115 19.185 105-109 26.645000000000003 28.549999999999997 25.619999999999997 19.185 110-114 26.855 28.205000000000002 26.035000000000004 18.905 115-119 27.395000000000003 28.79 24.27 19.545 120-124 27.465 28.315 25.790000000000003 18.43 125-129 26.735 29.625 25.869999999999997 17.77 130-134 27.345000000000002 28.38 24.834999999999997 19.439999999999998 135-139 27.01635081754088 29.29146457322866 25.731286564328215 17.960898044902248 140-144 27.927792779277926 28.717871787178716 25.447544754475448 17.906790679067907 145-149 28.477119279819956 28.91722930732683 24.98624656164041 17.619404851212803 150-151 28.42855356919615 28.078509813726715 25.565695711963997 17.92724090511314 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 1.0 3 1.5 4 1.0 5 1.0 6 3.0 7 2.5 8 1.0 9 1.5 10 1.0 11 1.0 12 1.5 13 3.0 14 2.5 15 1.5 16 1.0 17 0.5 18 1.0 19 2.0 20 3.0 21 3.0 22 4.0 23 4.0 24 2.5 25 2.0 26 4.0 27 7.5 28 8.0 29 9.5 30 15.5 31 15.0 32 10.0 33 16.5 34 32.5 35 48.5 36 64.5 37 81.0 38 98.0 39 104.5 40 147.0 41 196.0 42 209.5 43 247.0 44 288.0 45 293.5 46 279.5 47 266.5 48 248.5 49 214.5 50 173.5 51 160.5 52 130.5 53 89.0 54 74.0 55 59.5 56 49.5 57 36.5 58 33.5 59 34.0 60 29.0 61 21.5 62 13.5 63 11.0 64 9.0 65 7.0 66 7.5 67 7.0 68 3.5 69 2.0 70 1.5 71 2.0 72 1.5 73 1.0 74 0.5 75 0.0 76 1.0 77 3.0 78 3.0 79 1.0 80 1.5 81 3.0 82 2.5 83 3.0 84 5.5 85 6.0 86 5.5 87 4.0 88 2.5 89 3.0 90 3.0 91 2.0 92 2.0 93 1.5 94 0.5 95 2.5 96 3.5 97 2.0 98 1.5 99 3.5 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.005 140-144 0.01 145-149 0.025 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.975 #Duplication Level Percentage of deduplicated Percentage of total 1 65.4296087131908 40.550000000000004 2 20.129084308188787 24.95 3 8.26946349334409 15.375 4 3.509479628882614 8.7 5 1.653892698668818 5.125 6 0.44372730939895116 1.6500000000000001 7 0.24203307785397335 1.05 8 0.08067769261799113 0.4 9 0.12101653892698667 0.675 >10 0.12101653892698667 1.525 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 35 0.8750000000000001 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 14 0.35000000000000003 No Hit GGATCCACCACCACCACCCACAGCTCCACTGCTCGGTCCAGAAAGCCATG 12 0.3 No Hit AGAAAACTCAGAACAAGCTCATAAGAGGCCAAGAAGAGAAAAATGCTAGT 9 0.22499999999999998 No Hit AGTTCGATGTATGTGTCGGTTTTTATTGCATTAGCGAGCGAAGGAACGGA 9 0.22499999999999998 No Hit CAGCATGATGAGACTGTCACAAATGATGAAATTGCTGCTGATCTAAAGGA 9 0.22499999999999998 No Hit AGCTAGTGTTAGACAGGTTCTTGTTAGACAGAGAGATGGAGAAGAAATGC 8 0.2 No Hit TGCTGATGTTCCAGTTGAAGCATACAAAACACTCACTTCTTTGAAGAGTG 8 0.2 No Hit ACAACACTCCAAAAACCGTCTCCTCTGATAGCAAGGGTGATGTTAAAGTC 7 0.17500000000000002 No Hit GTGGCAGAGGAGGCCGAGGAGAGGCTATGCTCCCAGCTGGGTGAGCTCGA 7 0.17500000000000002 No Hit GCCCTACTGAAAATGGCTCGGAGGCAGCTGCTAATGATGATAACAGTTGG 7 0.17500000000000002 No Hit GATGAAACGATAATGGCAGGGAGCACGGCGGCGGCGACTCTGCTGACGGC 7 0.17500000000000002 No Hit ACAGGGTTCAGGGAAGTGGCAGCATTCCTTCTTGACCATGACCACTTTGC 7 0.17500000000000002 No Hit ACTAATTCAGACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATAGTT 7 0.17500000000000002 No Hit CAACCATTTCGTCAAGGAGATGAACCAGAAGGGCTATACAGTGTTTGGTT 6 0.15 No Hit AAAGAAGGCGATTGTCGAAGCTAAACGCGGGGAAGATCTTCTCAAGGCTG 6 0.15 No Hit TTCCCTGTACAGATGTTGACATATAATTGAAAGGTTAAACTGATCATGAT 6 0.15 No Hit CTCTCACCTCTCTAACATTGGTTCTACCTCCACTGCTGCCTCTATTGCAA 6 0.15 No Hit GGTTCTTCAATATCGCTACAAAAGGATTTGGGTTCAATGAGGATTAGATC 6 0.15 No Hit AATAACGGCAGCACATTTTGAGGAGTCAATGAAATTTGCACGTCGAAGTG 6 0.15 No Hit CATAGGGGAGGACCTCCATTATCTCTCAAAAAGTAAAAGCCTGTGTTGAA 6 0.15 No Hit AATGATACTGGGGTTTTGAACAATGGATTGTGGACCAACCAGACCAACCA 6 0.15 No Hit GGAAATGGGAATTGGATTCTTAGGAATTGGCTTCCAGCCCAAATGGGGAC 6 0.15 No Hit CAGCACTCTCGCTTTGGTGGCTCGTGTTTCTGCTTTCTCTTTGGGTCTCG 6 0.15 No Hit TGTTACCCAAGCTGTTCGTTCACGCACTTACCTTGCTGGGTTTCATCCGG 6 0.15 No Hit CTCTCATCAGAAAAGCTTAGACTCATCCGATTTAGGGTTTCGCTTCATAT 5 0.125 No Hit GTGGCGTTATTGTCAGTGACCGAGAAAATGAACAAAGCCGGCGGAATTGC 5 0.125 No Hit GTTACAAAGAAGCCATCCCTGAAAGTGTGTCGAGGCATGGAACTAAAGCT 5 0.125 No Hit GGTAACCAAGCCAATCAGGGCAACCAGGGCAATCAAGGAGGCTTTGGAGG 5 0.125 No Hit GAGGAACATCTAAAGCAATTGGAGGAGGAGCTTGAGGAAAAGGCAAAGGG 5 0.125 No Hit GATTGGGCTTGGGATCCACCAGACATCGCAAAAGCTGGCAAAGCTCGCAA 5 0.125 No Hit AGGACAGCTCCTCCTCCTCATTTTAATTTCCCTGTACTCTCTGCTGAGAC 5 0.125 No Hit CAGAGATCGATCCAGCTGAGCTTAGGGAAATGGTGTTGAGCCATAGGGTT 5 0.125 No Hit GACACTGTCAAGAGAACTCGTTCTACGGTATGGATGAAAAAAGATGGCCT 5 0.125 No Hit CTTCGTCTTGAAATATAGAGGCAAGAAGCAAAACCTCCACTGGCTCTCTA 5 0.125 No Hit GATTAGGTGTTGCTGGTTGGGGAGTTGGTGGGATAGAAGCCGAAGCTGCA 5 0.125 No Hit CAATTTGTTAAAAATCTCCAACGCCAGGTTTCACTTTACATTCCCAGAGA 5 0.125 No Hit GCTGACTTCTCTTTGTAGTAGCTTGTTCCTTTGAAAATGGAAAGAGATTT 5 0.125 No Hit AAAAGAGCAGGCCCCACACTCTGTTTGCCAGTGGACCTCCAACACTTGCC 5 0.125 No Hit CATTGATTTTCCCTGTGTGGAATTCCTTGTGATCTAATTGGAGAGTTCTG 5 0.125 No Hit CCTGCTTATACTGAAGCTGCAGTTTCAAACACAAATACACCCCTTGAACC 5 0.125 No Hit ATCTCACAAGAAGAGATGAGAGCTGTTGCTGACTATATTAAGCGTCAAGG 5 0.125 No Hit GAGCACAGGTCAGAATGGGCAGATAACAATATCGATGCCTATGCAGCTGC 5 0.125 No Hit TATAATGAAGCATTTCTCGGGAAGCCAAATGGTGAATATTGTAATTGGAT 5 0.125 No Hit AATCTTGGTGTGGGTTACTTTGAGAACAGATTGGGATAAAGAGGTGGAGA 5 0.125 No Hit GATAAAGATTGTGGCCACATGCCTGGTGGTTTCCGTTGGCATATCCCTCT 5 0.125 No Hit GCCAGGAAAATGGCTTGGTCCCCATTGTAGAGCCTGAAATCTTGGTTGAT 5 0.125 No Hit GCCCTGCTTAATTAATCAGGTTGTGTTGAAACATGCAGAGGGTAACATGT 5 0.125 No Hit GGTGGATTGTGATACCAGTTATAATGAAGGGTGCAATGGTGGTCTTATGG 5 0.125 No Hit CAAACTACACGGAGCTGACCCAGCTATATGCAAAATACAAGGATCAAGGT 5 0.125 No Hit GGTAGATGGAGCAACAGCCAAGTTTGATAAGCATGCTCCTCCTGTTGCGA 5 0.125 No Hit AGCTTGAGCAAATTCAGTTTCTAAGCAAAAGCTTTCCAGGCCCCTTTATC 5 0.125 No Hit GAGAACTTTGCATTGCCCAGGATCACAAATCACTGCTGGAGAAGCAAATT 5 0.125 No Hit AAGTGGAGTCCCAGCCATGTTTTCCCAGTTCATAACTGACTTGCCTACAT 5 0.125 No Hit GACAGCTTGAGGTTTATAGATCTGGAGGCGAAGAGTTCCGAAACGGAAAG 5 0.125 No Hit GGACAGGCTATTTCCCTGTGAAGTGGCACATTGTGAAGGATGTCCCCAAC 5 0.125 No Hit GCCAATTTTCAATCTCCCTCGCTCCCTCAACACCTTCTCTTGTCCACAGA 5 0.125 No Hit CAATATCCATGGACATGAAGGACAAGAAATTGACGGTGATTGGGGACATC 5 0.125 No Hit GAATGAGCGGAAGACTATTGATCTTGAACAAGGATGGGAGTTTATGCAGA 5 0.125 No Hit GGTCCAACTCTCTGTTCCCTTATGAACTTCAAGAGGTCGTTCATGCAAAT 5 0.125 No Hit CCCCATAACCAGATTGCTTGCGATCATAACGACGTTTACCCTGAGCAGCA 5 0.125 No Hit GGACACACTCTCGTGCATATGGAGTTCAAGATTGTTTGCCCTGGTGAAGG 5 0.125 No Hit ACGAAAGACTGGAGGGAAGAAGGCATAGTCAGTCCCGTTAAGAATCAAGG 5 0.125 No Hit CGTAAGCCTGTTGGTGAAATTCACCTCAAGGTATCATCAATCCGCGAGAG 5 0.125 No Hit GCTAATGTTAGTCATTTTGTCAAGAGAAACATCAACTGAGGGGAAATAAA 5 0.125 No Hit CCATCACCTTGGAGGTGGAGAGCTCCGACACCATTGATAATGTCAAGGCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.075 0.0 0.0 0.0 0.0 48-49 0.075 0.0 0.0 0.0 0.0 50-51 0.075 0.0 0.0 0.0 0.0 52-53 0.075 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.0875 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.125 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.15 0.0 0.0 0.0 0.0 66-67 0.175 0.0 0.0 0.0 0.0 68-69 0.1875 0.0 0.0 0.0 0.0 70-71 0.275 0.0 0.0 0.0 0.0 72-73 0.325 0.0 0.0 0.0 0.0 74-75 0.3375 0.0 0.0 0.0 0.0 76-77 0.3875 0.0 0.0 0.0 0.0 78-79 0.4 0.0 0.0 0.0 0.0 80-81 0.55 0.0 0.0 0.0 0.0 82-83 0.65 0.0 0.0 0.0 0.0 84-85 0.7 0.0 0.0 0.0 0.0 86-87 0.75 0.0 0.0 0.0 0.0 88-89 0.9125 0.0 0.0 0.0 0.0 90-91 1.2374999999999998 0.0 0.0 0.0 0.0 92-93 1.4625 0.0 0.0 0.0 0.0 94-95 1.875 0.0 0.0 0.0 0.0 96-97 2.225 0.0 0.0 0.0 0.0 98-99 2.5625 0.0 0.0 0.0 0.0 100-101 2.8875 0.0 0.0 0.0 0.0 102-103 3.3 0.0 0.0 0.0 0.0 104-105 3.9250000000000003 0.0 0.0 0.0 0.0 106-107 4.0875 0.0 0.0 0.0 0.0 108-109 4.449999999999999 0.0 0.0 0.0 0.0 110-111 5.0375 0.0 0.0 0.0 0.0 112-113 5.550000000000001 0.0 0.0 0.0 0.0 114-115 5.875 0.0 0.0 0.0 0.0 116-117 6.4125 0.0 0.0 0.0 0.0 118-119 7.0125 0.0 0.0 0.0 0.0 120-121 7.65 0.0 0.0 0.0 0.0 122-123 8.2375 0.0 0.0 0.0 0.0 124-125 8.8875 0.0 0.0 0.0 0.0 126-127 9.95 0.0 0.0 0.0 0.0 128-129 10.712499999999999 0.0 0.0 0.0 0.0 130-131 11.15 0.0 0.0 0.0 0.0 132-133 11.625 0.0 0.0 0.0 0.0 134-135 12.5375 0.0 0.0 0.0 0.0 136-137 13.337499999999999 0.0 0.0 0.0 0.0 138-139 14.25 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAACCC 10 0.006830828 145.0 4 GTTCTTC 10 0.006830828 145.0 2 AATATCG 10 0.006830828 145.0 9 GCTCATA 10 0.006830828 145.0 1 >>END_MODULE Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711472 spots for SRR26075408.sra Written 3711472 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra Read 3711461 spots for SRR26075408.sra Written 3711461 spots for SRR26075408.sra SRR ids: ['SRR26075408.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_zocxl2xh SRR26075408.sra spots: 74229231 blocks: [[1, 3711461], [3711462, 7422922], [7422923, 11134383], [11134384, 14845844], [14845845, 18557305], [18557306, 22268766], [22268767, 25980227], [25980228, 29691688], [29691689, 33403149], [33403150, 37114610], [37114611, 40826071], [40826072, 44537532], [44537533, 48248993], [48248994, 51960454], [51960455, 55671915], [55671916, 59383376], [59383377, 63094837], [63094838, 66806298], [66806299, 70517759], [70517760, 74229231]] SRR26075408 file size 27423916 SRR26075408 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075408 SRR26075408_1.fastq SRR26075408_2.fastq Input file: SRR26075408_1.fastq Paired file: SRR26075408_2.fastq trimmed: SRR26075408-trimmed-pair1.fastq, SRR26075408-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:52:35 2025 >> started Wed Feb 12 01:54:36 2025 >> done (121.036s) 74229231 read pairs processed; of these: 722 ( 0.00%) short read pairs filtered out after trimming by size control 782859 ( 1.05%) empty read pairs filtered out after trimming by size control 73445650 (98.94%) read pairs available; of these: 14618188 (19.90%) trimmed read pairs available after processing 58827462 (80.10%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 67 0.00% 19 64 0.00% 20 83 0.00% 21 129 0.00% 22 130 0.00% 23 156 0.00% 24 194 0.00% 25 224 0.00% 26 266 0.00% 27 282 0.00% 28 275 0.00% 29 313 0.00% 30 269 0.00% 31 305 0.00% 32 338 0.00% 33 350 0.00% 34 320 0.00% 35 293 0.00% 36 362 0.00% 37 472 0.00% 38 656 0.00% 39 504 0.00% 40 709 0.00% 41 605 0.00% 42 655 0.00% 43 673 0.00% 44 708 0.00% 45 821 0.00% 46 940 0.00% 47 956 0.00% 48 1192 0.00% 49 1354 0.00% 50 1548 0.00% 51 1707 0.00% 52 1918 0.00% 53 1946 0.00% 54 2332 0.00% 55 3015 0.00% 56 2969 0.00% 57 3387 0.00% 58 4442 0.01% 59 4253 0.01% 60 5107 0.01% 61 5583 0.01% 62 6366 0.01% 63 7586 0.01% 64 7671 0.01% 65 8250 0.01% 66 9170 0.01% 67 10152 0.01% 68 11498 0.02% 69 13053 0.02% 70 14882 0.02% 71 17171 0.02% 72 19795 0.03% 73 21820 0.03% 74 24127 0.03% 75 26049 0.04% 76 28162 0.04% 77 30434 0.04% 78 32580 0.04% 79 36412 0.05% 80 40559 0.06% 81 44488 0.06% 82 48939 0.07% 83 54341 0.07% 84 58494 0.08% 85 63014 0.09% 86 65682 0.09% 87 66949 0.09% 88 70414 0.10% 89 75288 0.10% 90 79336 0.11% 91 86300 0.12% 92 92038 0.13% 93 99642 0.14% 94 105628 0.14% 95 110778 0.15% 96 114006 0.16% 97 116847 0.16% 98 118956 0.16% 99 124201 0.17% 100 128971 0.18% 101 133421 0.18% 102 141693 0.19% 103 146773 0.20% 104 154818 0.21% 105 160584 0.22% 106 163525 0.22% 107 166402 0.23% 108 169215 0.23% 109 171255 0.23% 110 173509 0.24% 111 180285 0.25% 112 188580 0.26% 113 191863 0.26% 114 202189 0.28% 115 207218 0.28% 116 213372 0.29% 117 217828 0.30% 118 218811 0.30% 119 219975 0.30% 120 223186 0.30% 121 228097 0.31% 122 233762 0.32% 123 243104 0.33% 124 250230 0.34% 125 253850 0.35% 126 261954 0.36% 127 265395 0.36% 128 267354 0.36% 129 267434 0.36% 130 270824 0.37% 131 271459 0.37% 132 278663 0.38% 133 284864 0.39% 134 290201 0.40% 135 298403 0.41% 136 303311 0.41% 137 303818 0.41% 138 307589 0.42% 139 309991 0.42% 140 313385 0.43% 141 312327 0.43% 142 317252 0.43% 143 322837 0.44% 144 330974 0.45% 145 336316 0.46% 146 337831 0.46% 147 339072 0.46% 148 342831 0.47% 149 340468 0.46% 150 347169 0.47% 151 58827462 80.10% 73445650 reads passed initial QC criterion=sequence-density sequence-density=0.42 sequence-density-rank=1 fanout-score=28.94 fanout-score-rank=7 prefix-density=0.39 prefix-fanout=28.9 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGGACTTATCTCGTATGCCGTCTTCTGCTTG criterion=fanout-score sequence-density=0.06 sequence-density-rank=34 fanout-score=428.53 fanout-score-rank=1 prefix-density=0.83 prefix-fanout=28.4 sequence=TCATCATCACCACCATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTC criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=2.64 fanout-score-rank=36 prefix-density=0.27 prefix-fanout=2.6 sequence=ATGTACCCTGACTTAGGTTTCTCAGA criterion=fanout-score sequence-density=0.09 sequence-density-rank=14 fanout-score=379.03 fanout-score-rank=1 prefix-density=1.08 prefix-fanout=32.4 sequence=AAGAAGAAGAAA SRR26075408 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:55:17 Started mapping on | Feb 12 01:55:17 Finished on | Feb 12 02:05:48 Mapping speed, Million of reads per hour | 419.02 Number of input reads | 73445650 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 64798839 Uniquely mapped reads % | 88.23% Average mapped length | 288.79 Number of splices: Total | 59376093 Number of splices: Annotated (sjdb) | 57915142 Number of splices: GT/AG | 58268508 Number of splices: GC/AG | 838856 Number of splices: AT/AC | 67425 Number of splices: Non-canonical | 201304 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.93 Insertion rate per base | 0.02% Insertion average length | 2.54 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 2011397 % of reads mapped to multiple loci | 2.74% Number of reads mapped to too many loci | 240224 % of reads mapped to too many loci | 0.33% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.04% % of reads unmapped: other | 0.66% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 6635414 6635414 6635414 N_multimapping 2011397 2011397 2011397 N_noFeature 1672173 63965307 2139014 N_ambiguous 747829 4656 378643 UnstrandedReadsAssigned:62378837 PositiveStrandReadsAssigned:828876 NegativeStrandReadsAssigned:62281182 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=148 echo kmer=143 SRR26075408 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075408-trimmed-pair1.fastq SRR26075408-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 73,445,650 reads, 63,753,124 reads pseudoaligned [quant] estimated average fragment length: 201.556 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,198 rounds 52401 SRR26075408.ke.tsv 34699 SRR26075408.se.tsv 87100 total ==> SRR26075408.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1817.44 7849 60.2156 Potri.005G024800.1.v4.1 1035 834.444 2262 37.7964 Potri.004G059700.1.v4.1 961 760.449 37 0.678402 Potri.007G009000.2.v4.1 1416 1215.44 0 0 Potri.003G141000.2.v4.1 2943 2742.44 2460.68 12.5104 Potri.016G087400.1.v4.1 270 97.6024 6627 946.699 Potri.015G069301.1.v4.1 564 364.973 0 0 Potri.010G195200.1.v4.1 1773 1572.44 1258 11.1548 Potri.012G127500.1.v4.1 977 776.444 18759 336.864 ==> SRR26075408.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 1363 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 1569 Potri.001G212900.v4.1 47 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 2241 SRR26075408 completed mapping pipeline successfully