Starting /dee2/code/volunteer_pipeline.sh SRR26075409 current disk space = 3050819256320 free memory = 1497173572 SRR26075409 SRAfilesize d7c18ad6405376242e309fbecde5cd2d SRR26075409.sra SRR26075409.sra file validated SRR26075409 is paired end SRR26075409 is conventional basespace SRR26075409 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075409_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.63775 37.0 37.0 37.0 37.0 37.0 2 36.5635 37.0 37.0 37.0 37.0 37.0 3 36.658 37.0 37.0 37.0 37.0 37.0 4 36.7705 37.0 37.0 37.0 37.0 37.0 5 36.744 37.0 37.0 37.0 37.0 37.0 6 36.7035 37.0 37.0 37.0 37.0 37.0 7 36.662 37.0 37.0 37.0 37.0 37.0 8 36.6815 37.0 37.0 37.0 37.0 37.0 9 36.6395 37.0 37.0 37.0 37.0 37.0 10-14 36.668400000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.6369 37.0 37.0 37.0 37.0 37.0 20-24 36.566199999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.5168 37.0 37.0 37.0 37.0 37.0 30-34 36.4542 37.0 37.0 37.0 37.0 37.0 35-39 36.4454 37.0 37.0 37.0 37.0 37.0 40-44 36.30149999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.021499999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.138 37.0 37.0 37.0 37.0 37.0 55-59 35.883199999999995 37.0 37.0 37.0 37.0 37.0 60-64 35.857299999999995 37.0 37.0 37.0 37.0 37.0 65-69 35.6721 37.0 37.0 37.0 37.0 37.0 70-74 35.968199999999996 37.0 37.0 37.0 37.0 37.0 75-79 36.1747 37.0 37.0 37.0 37.0 37.0 80-84 36.135600000000004 37.0 37.0 37.0 37.0 37.0 85-89 36.0139 37.0 37.0 37.0 37.0 37.0 90-94 35.9598 37.0 37.0 37.0 37.0 37.0 95-99 36.0578 37.0 37.0 37.0 37.0 37.0 100-104 35.9841 37.0 37.0 37.0 37.0 37.0 105-109 35.845099999999995 37.0 37.0 37.0 37.0 37.0 110-114 35.800200000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.611200000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.72619999999999 37.0 37.0 37.0 37.0 37.0 125-129 35.5826 37.0 37.0 37.0 37.0 37.0 130-134 35.4507 37.0 37.0 37.0 37.0 37.0 135-139 35.2895 37.0 37.0 37.0 29.8 37.0 140-144 35.0043 37.0 37.0 37.0 25.0 37.0 145-149 35.0436 37.0 37.0 37.0 25.0 37.0 150-151 34.78725 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 2.0 23 2.0 24 2.0 25 5.0 26 12.0 27 16.0 28 17.0 29 24.0 30 25.0 31 38.0 32 67.0 33 156.0 34 175.0 35 396.0 36 2860.0 37 202.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.61702127659574 13.11639549436796 12.841051314142678 40.42553191489361 2 20.724999999999998 16.675 33.275 29.325000000000003 3 18.925 19.0 29.425 32.65 4 21.975 24.65 21.85 31.525 5 24.625 31.0 23.95 20.424999999999997 6 23.525 34.449999999999996 23.25 18.775 7 14.725 29.15 39.475 16.650000000000002 8 18.95 30.075000000000003 28.65 22.325 9 18.7 22.650000000000002 34.849999999999994 23.799999999999997 10-14 19.830000000000002 30.475 25.695 24.0 15-19 20.544999999999998 27.065 27.665 24.725 20-24 20.355 28.1 27.365000000000002 24.18 25-29 20.565 28.005000000000003 27.175 24.255 30-34 19.68 29.985 27.3 23.035 35-39 20.794999999999998 28.749999999999996 27.095000000000002 23.36 40-44 19.465 28.994999999999997 27.800000000000004 23.74 45-49 20.41 27.3 28.64 23.65 50-54 20.47 26.695 28.410000000000004 24.425 55-59 20.73 26.47 27.965 24.834999999999997 60-64 20.93 27.155 27.905 24.01 65-69 20.26 29.909999999999997 26.865 22.965 70-74 22.395 26.669999999999998 27.63 23.305 75-79 21.645 26.3 26.845000000000002 25.21 80-84 22.62 27.950000000000003 25.89 23.54 85-89 22.015 28.065 26.479999999999997 23.44 90-94 22.905 26.86 26.740000000000002 23.494999999999997 95-99 22.82 26.97 27.73 22.48 100-104 23.04 27.015 25.805 24.14 105-109 22.585 27.250000000000004 27.02 23.145 110-114 22.89 28.17 25.69 23.25 115-119 22.919999999999998 27.21 26.369999999999997 23.5 120-124 22.895 26.69 26.55 23.865 125-129 22.795 27.334999999999997 26.165 23.705000000000002 130-134 23.5 26.76 26.810000000000002 22.93 135-139 23.445 26.779999999999998 26.575 23.200000000000003 140-144 23.76 26.505000000000003 25.474999999999998 24.26 145-149 24.145 26.665 25.41 23.78 150-151 23.674999999999997 26.637499999999996 25.912499999999998 23.775 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 1.5 23 1.5 24 3.0 25 3.0 26 2.5 27 5.0 28 6.5 29 14.0 30 17.0 31 13.0 32 22.5 33 38.5 34 38.0 35 57.5 36 91.5 37 94.5 38 107.5 39 140.5 40 179.0 41 196.5 42 215.5 43 261.0 44 268.5 45 286.5 46 284.5 47 237.0 48 232.5 49 234.0 50 195.5 51 139.5 52 110.5 53 86.5 54 72.0 55 54.5 56 35.5 57 31.5 58 27.0 59 25.5 60 13.0 61 13.5 62 14.0 63 7.0 64 8.0 65 11.0 66 19.5 67 25.0 68 21.5 69 17.0 70 10.5 71 3.5 72 0.5 73 1.5 74 2.0 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.125 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.800000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 59.09498207885304 32.975 2 21.729390681003586 24.25 3 10.528673835125447 17.625 4 5.10752688172043 11.4 5 1.478494623655914 4.125 6 0.8512544802867384 2.85 7 0.492831541218638 1.925 8 0.4480286738351254 2.0 9 0.13440860215053765 0.675 >10 0.08960573476702509 0.775 >50 0.044802867383512544 1.4000000000000001 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGCCTTATCTCGTAT 56 1.4000000000000001 TruSeq Adapter, Index 5 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGCCTTATCGCGTAT 16 0.4 TruSeq Adapter, Index 5 (97% over 37bp) GTCATAACAACCAGGCCTGCCCCGGGGCGAGCGCTAGGCGCTTGATCGTT 15 0.375 No Hit CACCGACTTACATTACACAAAGGACGGACCAGAAGAGTTTATCACTATGT 9 0.22499999999999998 No Hit ATCCAGTCTATTCCTACAGAGGAGTGAAAAATTCTTCAGATATATAGCTG 9 0.22499999999999998 No Hit CACTCCCAGATGCAAAAATTTGATCCACATAATAGGCTATACAAAGCACC 9 0.22499999999999998 No Hit AGTCAGCCCAGACAGTGTTTGCAGTTGCAGGAATTGATGAGGAAGAGGAG 8 0.2 No Hit CTGCAGAACACAAATTTTAAGAAAAAATGTCAAACAAAATCTTGAATGCC 8 0.2 No Hit CTCCCATTTTTTCCAATGTAGCAGCAGATGGGCCAAACAGGGGGTTTTCC 8 0.2 No Hit GCTGGAAACCAATGGGGCTGATTCAGGCACTTCAACGCTAAAGACCTTAG 8 0.2 No Hit GGAGGGGAATAAAAGAAGAAAATTTCAAGGTTGAAGTAATTAAGAACATG 8 0.2 No Hit GTTGAAAATTCCAAGGTTGGCGGGGATTAAAAAACAATGTCCTTGCTGAC 8 0.2 No Hit CTCATCATTTTTGACATCTAATGCTTGAGGTTGTTGCGCACACACCCCAA 8 0.2 No Hit GTAGACAACAGAATCTGCCTGGTTCTTTGTGTCAATGGCATCTCTCTTCT 8 0.2 No Hit CCTTGATTTGTGGGGTCCAGATGAGGGTTTGGGTGCAAATGCACCACCAA 8 0.2 No Hit AGGGGAAATCCTAAAAAGGCCAGCAATTATGGGAAAAAAAGCAAAACCCA 8 0.2 No Hit TGGCTCTGAGTTCAGGGCGTCTTTGGTCGGATTACAGAGGCCGAGGGGCG 7 0.17500000000000002 No Hit CGGGATCAAGTTGGTAAAAAACTGGTAAGACCATCTGTTGTTTGGTCTCC 7 0.17500000000000002 No Hit ATCCTGTCTCGTCACGGTCCATTTTTTGCTTCTCCTGGTTAAACAATTAA 7 0.17500000000000002 No Hit GTGGATGGTTGTGCTCTCCTTCATAAGTTGCCACTAGAACAGATTGGTCA 7 0.17500000000000002 No Hit CAACTTTACGCGCAGTAGGGTGGTCCCGGTGCTCACACAGCCATATTCCC 7 0.17500000000000002 No Hit CCAATAAGAATCAGCAATTCAGGAAGAAATATGACAAGGAGTAGTAGTGT 7 0.17500000000000002 No Hit CCAGTTTCTCAAGAGAAACAGACCATCACAAGACAGTAAACATCCTAACG 7 0.17500000000000002 No Hit CTGATGAAAACTGACTCAAATGATCAATTGCATCCTTAAATGCTTTAGTG 7 0.17500000000000002 No Hit CGCCAGCTAGCCATGGCTCAAAATCCGTAGCCCTTGAGCTCCTCTTCAAG 7 0.17500000000000002 No Hit GCTAGCCTTGCTGTGATAGATATATGGCAAACCAGTCTTGACTCCTAGTC 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGCCTTATCTCGTTT 7 0.17500000000000002 TruSeq Adapter, Index 5 (97% over 37bp) TGAAAAAAAAAAAAGGAAGTATAGGCATGTAGGAAACCCTCTTATGAATC 6 0.15 No Hit ACCTTCTTTTCCTTTGTAGCTCGGGATCATTGAAACTCCAGCTTTTTGAA 6 0.15 No Hit ATTTCCATCATGACACCCTTAACTTCAGAGACCTGAGCTTTCACTTTGGC 6 0.15 No Hit CATAATTTTTGTTATCATAAGCTAGTGTGAAGAACACACCAAACCCATAA 6 0.15 No Hit TTCCAGTTTTATCCAATATCTTATCAACCAGATCCCCGTCAGCCAAATCA 6 0.15 No Hit TCCTACTGTAATTATTTCTGTGTTACATAGCTTTCTCAGTTTGCTCACTA 6 0.15 No Hit CCTCCCTATACTAGACCCATAACTCCTAGCTCCAACATCAACATACACAT 6 0.15 No Hit CCACTCTTCGCAGCTGAGCAACATATTCTGAGATCTTTTTGATAGCATCC 6 0.15 No Hit CTTCTCTCTCAGCTCCGGGTCCCTTCGAAGGTGATCAAGAATGAGAAGTC 6 0.15 No Hit ATTTATAGCACTGCCTAAAGAATCCAGTGCAGGAGCACACTGGTGCTGGC 6 0.15 No Hit CAGCAATGTGACAGGTGTGGCAGTCAAGGACTGGAGCGTAACCGTTTCCG 6 0.15 No Hit AGAATGTGTGACTTGTACATGCACCTCCCTAGGGGGCATAAAAGGCTTCT 6 0.15 No Hit TACAGAAGATTATGCGTGCCCATAGTTCTTTGGATGCAGAAATAATCAAA 6 0.15 No Hit GCGACAAAGACCGAGCCCAAGGAAGGATAGATCCCGGCGGCAGCGGCGGT 6 0.15 No Hit CACAAACAGCCTCCTCATTCCTTCTTTCCAATCCCATGAGACATGTTATA 6 0.15 No Hit CTCCGTATGCTTTATTTCGAACGTAAAAATCAGAAGTAGAACCCTTCGAT 6 0.15 No Hit GGATCGAGGTATCCAAAACTCCCTTTCACTGCTGTGCTCACATGGGTCTG 6 0.15 No Hit ATCATCGAACGCCACATACCTGCTATTATAATCAACGTCCCCTGCTCCCA 6 0.15 No Hit GGATCTGAGCCCACTTGAAGAGTTTCCAGTAAATTTTCAGTCATTTCCTC 6 0.15 No Hit GCTTTTCCCTGTGAACAGCTCTAGCAGCATAACTCCATAGCTGTATACAT 5 0.125 No Hit CTCAAGAGAAACAGACCATCACAAGACAGTAAACATCCTAACGATGGGCT 5 0.125 No Hit CTACATATAAGGCAGCTTGCTTACAATCCCACAGAACCCAGAAACACACA 5 0.125 No Hit TCCTTTTCTTCTTCCCATTTTCATCACTCTTTGCATGTGCAGGACCACCC 5 0.125 No Hit GGAACAACTGGAGGAACAGAAGGCAGGATAGATGGTATAGAAATACCAGC 5 0.125 No Hit CTCCTCCTCTTCTTCTGATTGTTATTGTTGTTGCTGTAGTCTTCGCCGCT 5 0.125 No Hit GTCTCGCTAATTAGTTGCTATAAGCTGTAACACTTGGTCTTGGTTCAAAT 5 0.125 No Hit CTAACACGGACACGATAAACCACGTAGCCCTGCTTGGCCTTATAGCCCAA 5 0.125 No Hit CAATCGAGCTCTGATTGCCTTCAAAAATGAGTAAATCCACTTGTCTGGAG 5 0.125 No Hit GATTTGCAAGAGAAAGTTCAGTGTCTGATATTTGAGACCCAACTCTTATA 5 0.125 No Hit CTTCCCTATCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATT 5 0.125 No Hit GCTGTCAATGGTATTTGCATGGGTTGGTTCTCTATACCGGGATGGTGGAG 5 0.125 No Hit CCCTCTTGTGGGCATTGCAAAGTAAGAATAGACCAGAGAAGAAATAGATT 5 0.125 No Hit GCTTGGATTGGTAGTCTTACCCATTGCATTGATTTGCAACAACCTGTATT 5 0.125 No Hit ATTCGAAGATGCTATCGGAATCCGGGAATCCATCCAACAAATTATCCCAA 5 0.125 No Hit TACGCCGATAACCCTGGATTCACCTTCAATGCCCCTTCTTCAAAATCAAA 5 0.125 No Hit CTTCAGTAGAATAAGACGCCAGATACCAACCCATTGTCCCCTGTTATGGT 5 0.125 No Hit TGTAAACTTTCCATTGATGGTCACAATGGACTTGGTTGAGCAGAGCTTTG 5 0.125 No Hit GTCGAGATAGCATGGGGACAAGCTCGTCACTTGAAGGCCTATCTGCAGGC 5 0.125 No Hit CTAGCATGATCACAAACATGTGATGCTTATTGGTCGAGATCGATGACCCC 5 0.125 No Hit GATGCTAGCCAGCCAAGATAAACATACAGGCATCGTATTCAACCAGATAT 5 0.125 No Hit ATTCCTTGGAGGATTTGTTGATTAGCGACTTGTGGATATGAGGGATCACA 5 0.125 No Hit GCGGAAGCGGTTTTTTTGGCGGTTGTGGCAGTGGAGCCGCCACGAGCAAG 5 0.125 No Hit GGGGGAGACAACTGCACTTCGGGTAGTGGAGGCAGTAATGGATGAAGTAG 5 0.125 No Hit GACCAGGAAAGCAGTCCCTAACACTCTTGGACTTGCCATTTCCAGATTAT 5 0.125 No Hit GTGACGGGGCTATAAAATAAATGAATCATACACAAATAATTAAAGATTTA 5 0.125 No Hit GTAATATTTCTCAAATTTTGACTCAAATAATCAGTAACCCTTAGAAGCTC 5 0.125 No Hit GTCAAGGTTACAGCCTTCTACTTGACAGCGTGGAGCACGCTGACTATGAT 5 0.125 No Hit TTCCAAAGACTACCAGCAACCAAGCCAAGTGCAAATCCAATACAAAGCTC 5 0.125 No Hit CATAACAAAAGCCAGTAAAGGCAACTAACATGAAACCCTAGTAGAAAATG 5 0.125 No Hit ATGCAATCTCCGGTGGGGGAGGAAGGTTAAGATTAAGATCAAGATCAACA 5 0.125 No Hit GCGGGAAAAAATCACTGTAAAGATCATCAAAACCCTGAAATATCCCATAT 5 0.125 No Hit AGTTATTATACCTGCAGACTCAGACAAACCAACAAATGAGCTCATTGGTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.1375 0.0 0.0 0.0 0.0 76-77 0.2875 0.0 0.0 0.0 0.0 78-79 0.38749999999999996 0.0 0.0 0.0 0.0 80-81 0.48750000000000004 0.0 0.0 0.0 0.0 82-83 0.525 0.0 0.0 0.0 0.0 84-85 0.5625 0.0 0.0 0.0 0.0 86-87 0.6375 0.0 0.0 0.0 0.0 88-89 0.8625 0.0 0.0 0.0 0.0 90-91 1.1625 0.0 0.0 0.0 0.0 92-93 1.35 0.0 0.0 0.0 0.0 94-95 1.7000000000000002 0.0 0.0 0.0 0.0 96-97 2.1625 0.0 0.0 0.0 0.0 98-99 2.325 0.0 0.0 0.0 0.0 100-101 2.5374999999999996 0.0 0.0 0.0 0.0 102-103 2.875 0.0 0.0 0.0 0.0 104-105 3.3125 0.0 0.0 0.0 0.0 106-107 3.8125 0.0 0.0 0.0 0.0 108-109 4.1 0.0 0.0 0.0 0.0 110-111 4.525 0.0 0.0 0.0 0.0 112-113 5.1 0.0 0.0 0.0 0.0 114-115 5.5 0.0 0.0 0.0 0.0 116-117 5.9375 0.0 0.0 0.0 0.0 118-119 6.3 0.0 0.0 0.0 0.0 120-121 6.637499999999999 0.0 0.0 0.0 0.0 122-123 6.9375 0.0 0.0 0.0 0.0 124-125 7.512499999999999 0.0 0.0 0.0 0.0 126-127 7.9875 0.0 0.0 0.0 0.0 128-129 8.7 0.0 0.0 0.0 0.0 130-131 9.2125 0.0 0.0 0.0 0.0 132-133 9.8375 0.0 0.0 0.0 0.0 134-135 10.712499999999999 0.0 0.0 0.0 0.0 136-137 11.425 0.0 0.0 0.0 0.0 138-139 12.4875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACGTAA 10 0.006830828 145.0 3 AAGAGTA 10 0.006830828 145.0 9 CCTCCTG 10 0.006830828 145.0 145 AGGAGAG 10 0.006830828 145.0 5 >>END_MODULE SRR26075409 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075409_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.34275 37.0 37.0 37.0 37.0 37.0 2 36.342 37.0 37.0 37.0 37.0 37.0 3 36.2515 37.0 37.0 37.0 37.0 37.0 4 36.406 37.0 37.0 37.0 37.0 37.0 5 36.3115 37.0 37.0 37.0 37.0 37.0 6 36.2165 37.0 37.0 37.0 37.0 37.0 7 36.1815 37.0 37.0 37.0 37.0 37.0 8 36.2845 37.0 37.0 37.0 37.0 37.0 9 36.2925 37.0 37.0 37.0 37.0 37.0 10-14 36.07940000000001 37.0 37.0 37.0 37.0 37.0 15-19 35.96249999999999 37.0 37.0 37.0 37.0 37.0 20-24 35.80479999999999 37.0 37.0 37.0 37.0 37.0 25-29 35.6154 37.0 37.0 37.0 37.0 37.0 30-34 35.4384 37.0 37.0 37.0 37.0 37.0 35-39 35.342600000000004 37.0 37.0 37.0 37.0 37.0 40-44 35.280499999999996 37.0 37.0 37.0 37.0 37.0 45-49 35.1315 37.0 37.0 37.0 37.0 37.0 50-54 35.0332 37.0 37.0 37.0 34.6 37.0 55-59 35.08540000000001 37.0 37.0 37.0 34.6 37.0 60-64 35.146699999999996 37.0 37.0 37.0 34.6 37.0 65-69 35.0081 37.0 37.0 37.0 34.6 37.0 70-74 34.9202 37.0 37.0 37.0 27.4 37.0 75-79 34.8463 37.0 37.0 37.0 25.0 37.0 80-84 34.9066 37.0 37.0 37.0 27.4 37.0 85-89 34.9482 37.0 37.0 37.0 27.4 37.0 90-94 34.8578 37.0 37.0 37.0 25.0 37.0 95-99 35.052099999999996 37.0 37.0 37.0 27.4 37.0 100-104 35.023199999999996 37.0 37.0 37.0 27.4 37.0 105-109 35.060700000000004 37.0 37.0 37.0 29.8 37.0 110-114 34.9735 37.0 37.0 37.0 25.0 37.0 115-119 35.0239 37.0 37.0 37.0 27.4 37.0 120-124 34.899950000000004 37.0 37.0 37.0 25.0 37.0 125-129 34.7997 37.0 37.0 37.0 25.0 37.0 130-134 34.794799999999995 37.0 37.0 37.0 25.0 37.0 135-139 34.73315 37.0 37.0 37.0 25.0 37.0 140-144 34.68235 37.0 37.0 37.0 25.0 37.0 145-149 34.61795 37.0 37.0 37.0 25.0 37.0 150-151 34.296375 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 10.0 14 11.0 15 8.0 16 18.0 17 9.0 18 13.0 19 4.0 20 14.0 21 14.0 22 10.0 23 22.0 24 25.0 25 30.0 26 38.0 27 24.0 28 18.0 29 19.0 30 22.0 31 30.0 32 52.0 33 92.0 34 171.0 35 665.0 36 2474.0 37 206.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.08427106776694 20.005001250312578 17.129282320580145 25.78144536134033 2 34.175 23.125 26.450000000000003 16.25 3 28.075 27.6 25.2 19.125 4 27.55 32.975 20.424999999999997 19.05 5 26.775 33.225 21.275 18.725 6 25.074999999999996 37.175000000000004 20.5 17.25 7 24.4 20.599999999999998 35.425000000000004 19.575 8 24.775 24.55 25.2 25.474999999999998 9 26.150000000000002 25.5 25.724999999999998 22.625 10-14 27.26 27.85 24.585 20.305 15-19 26.83 27.435 25.169999999999998 20.565 20-24 26.27 27.36 26.045 20.325 25-29 26.155 27.37 26.465 20.01 30-34 26.82 26.369999999999997 26.279999999999998 20.53 35-39 26.14 28.76 25.405 19.695 40-44 26.015 26.85 26.979999999999997 20.155 45-49 26.76 27.685 26.545 19.009999999999998 50-54 23.205000000000002 27.215 29.65 19.93 55-59 24.92 27.715 27.169999999999998 20.195 60-64 27.3 27.27 26.334999999999997 19.095000000000002 65-69 26.345000000000002 27.224999999999998 26.200000000000003 20.23 70-74 25.074999999999996 28.98 26.445 19.5 75-79 24.48 29.759999999999998 25.66 20.1 80-84 26.009999999999998 27.644999999999996 25.94 20.405 85-89 26.8 28.939999999999998 25.814999999999998 18.445 90-94 26.25 28.765 25.705 19.28 95-99 25.955000000000002 28.58 26.484999999999996 18.98 100-104 27.139999999999997 27.589999999999996 26.08 19.189999999999998 105-109 26.795 28.46 26.275 18.47 110-114 27.72 27.400000000000002 25.655 19.225 115-119 27.49 28.02 25.665 18.825 120-124 26.66133306665333 28.536426821341067 25.73628681434072 19.065953297664883 125-129 27.075 27.48 26.200000000000003 19.245 130-134 28.050000000000004 28.26 26.08 17.61 135-139 27.619142871430714 27.704155623343503 26.068910336550484 18.6077911686753 140-144 28.034205130769614 28.84432664899735 24.978746812021804 18.142721408211234 145-149 28.49212303075769 27.09677419354839 26.026506626656666 18.38459614903726 150-151 28.34104263032879 27.97849731216402 25.803225403175396 17.87723465433179 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.5 8 1.0 9 0.5 10 1.5 11 1.0 12 1.5 13 2.0 14 0.5 15 0.5 16 2.0 17 2.0 18 1.5 19 1.0 20 1.0 21 2.5 22 2.0 23 1.0 24 1.5 25 2.0 26 4.0 27 4.5 28 2.0 29 5.5 30 8.5 31 9.5 32 18.5 33 29.0 34 38.5 35 53.5 36 88.5 37 98.5 38 118.0 39 152.5 40 163.0 41 190.0 42 242.5 43 261.0 44 256.0 45 285.5 46 284.0 47 257.0 48 228.0 49 202.0 50 168.0 51 146.0 52 106.5 53 65.0 54 63.5 55 47.5 56 48.0 57 48.0 58 30.5 59 15.0 60 7.5 61 12.0 62 16.5 63 10.5 64 11.0 65 11.0 66 4.5 67 5.0 68 3.5 69 4.0 70 3.0 71 1.0 72 2.0 73 2.5 74 1.5 75 3.0 76 7.0 77 6.0 78 3.5 79 5.0 80 6.0 81 6.5 82 7.0 83 5.5 84 7.0 85 10.0 86 11.0 87 6.5 88 2.5 89 4.0 90 6.0 91 6.5 92 2.5 93 0.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.5 100 14.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.015 140-144 0.015 145-149 0.025 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.875 #Duplication Level Percentage of deduplicated Percentage of total 1 62.159827213822894 35.975 2 19.82721382289417 22.95 3 10.19438444924406 17.7 4 4.578833693304536 10.6 5 1.3822894168466522 4.0 6 0.8639308855291578 3.0 7 0.4319654427645789 1.7500000000000002 8 0.3023758099352052 1.4000000000000001 9 0.12958963282937366 0.675 >10 0.12958963282937366 1.95 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 40 1.0 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 23 0.575 No Hit GACGACCCCAGCGAGGCCGTGCGGGCGGTCACCGCCATGGTTGCCCCAGG 15 0.375 No Hit CTGCCATGCTTCCATGTTCTCTGCAAGCAGTCAATCATGAAGCTATCAAA 9 0.22499999999999998 No Hit TGTGTGTTTACAAAAGGAGATGGTCCTAATGGGAGAAGAGTGGATGGCAT 9 0.22499999999999998 No Hit GTGAAAATGGGATGAGCGACGTGATGATTATCATGCTTCTTGAATGGTTG 9 0.22499999999999998 No Hit AGGCACACATGGTTTTAGCATATTATGGCATCAAATTCACATGAAACTTT 8 0.2 No Hit CCTACAGCCAAGGAGCTCTACTCCAAGTACGAACCGAAGGCAGAACAAGC 8 0.2 No Hit CAGGGAAGAAGCAAGACATCACCATTACTGGTGCTAGCACCTTACCTAGT 8 0.2 No Hit CTTTATTGCTATATGTTTTTAATGCTCTCTGCATACGGAGTGATAGCTCT 8 0.2 No Hit AAATTATAGTACTGTTTTTTCATCTCTCTCAACCGGTGTAGGCTTAGAAG 8 0.2 No Hit CAAGAACCAGCAGCTCAAGCAGGAGATAAATGTAGATTTTCCTACATTGG 8 0.2 No Hit CAGCAGAAAAGCAATGGACTTCATGGATAATTCCCATGTTTGTTTGCAAA 8 0.2 No Hit TGCCTTCATTAGCTTCAGAGGTGCTGAGAGCAGAAAGGCGAGGATTTTGG 7 0.17500000000000002 No Hit CTGCTCCTTGGAAGCATACCCTCGAAGGTCCAGATGACATGCCGGCACAC 7 0.17500000000000002 No Hit ATATGCAGTTGATTTCGGAAGCCTATGATGTTTTGAAGAATGTTGGTGGG 7 0.17500000000000002 No Hit AAAGCATCAAACTCAAAAACTCTCTCACTCTCTCTGACTTCAAGGAGAGC 7 0.17500000000000002 No Hit CTGATTGATGACGAGGCACCAGAGAAATTGGACAACTCGAATATTCAGGA 7 0.17500000000000002 No Hit TGCATAATGTGTGGATGATGTATGATCCTTAATGGTTTTCAATTGCTTTC 7 0.17500000000000002 No Hit TCACCAGTCATCTTTATGATGCTTTGCAGCGAAACCAAATTGATGCTTAC 7 0.17500000000000002 No Hit CTAAAAATAATGTTAAGAATTTTTTGCTGGTTTTTTGCGACGTTGGTGTT 7 0.17500000000000002 No Hit TGGTGATACAAGCCTTATTGTGAAAGATGGATATCAATGGAGGAAATATG 7 0.17500000000000002 No Hit TGTGGATGCTGTTTTGACCATTCCAAAGGGCACCCTGTTCCCCATGTGTG 7 0.17500000000000002 No Hit GGCTGCCACAGCCCAGGCCAACGGTCTTAACAAGGAATTTGGGCCAAAAT 6 0.15 No Hit CTCTCAAGAGTCATCTCTATGGCTCAGGTTCTCCTACCTTGAGTTGGAAG 6 0.15 No Hit GGGAGATGCACTGTATGCAATGGAGCTTGCCTTGTCTTTGGAAAAACTAA 6 0.15 No Hit GGAGAAGCTCGTTGAGCACCAGAAATATTTCCAAAGCATTCACAAGCACA 6 0.15 No Hit CAACCACCACAACCGAACTTCTCTCGTCCTTATCTCCATTCTTCCATAGC 6 0.15 No Hit GGTTCGTCAAAATCTAACTGTAGCATTATGTGAAAGAAACCAATGATGCA 6 0.15 No Hit GGAGAATACATGCTCTGTTCCGGGGTATAAAAGGGATTTGGTGCGCAATG 6 0.15 No Hit AACAGCTTCCAAGTTTCATAGAGGAAACAAGCCCAGATCTTTCTCAAAAT 6 0.15 No Hit TGTCAATTTGTGTGCATGTAATTTTATTTTTTTCCTCTTTCCGATGGAGC 6 0.15 No Hit AAAACTCTCTGTCTGTCTCTCTCCCCCTCCTCTCTGACTGGCTTGTCTCT 6 0.15 No Hit GGACTGTAAAATGGGATTCATGGCACTATTAGCTGCTACTAAATAAAGGG 6 0.15 No Hit GGGAGGTCAAGGGTGAAGAAGAGACCGAGTTGGTCCGAGTTGACTCGGCG 6 0.15 No Hit CAAAAACTTTCTTTTACTTTCCCTTTCTTCAAATTTCAAAAACCACAGCA 6 0.15 No Hit GAAGCACCCAAGAAAGTTTTCTGGCTTCCCATCACCACATCCTGGTATAA 6 0.15 No Hit CAGAAACAAAACAGCAAGCAATGGCTCTCAAGCTCAATCCTTTTGTCTCT 6 0.15 No Hit GGGGAAGGGTTGATAAACGAGCTGTGTAATGGGTTTCAGCTGTTGATGGA 6 0.15 No Hit GAAGGGGATTGCTATAAAACAAAAATCCTCAAGTGAAGAAACCAAAATGG 6 0.15 No Hit TGACGAGAAAGCCAAGACAAAAGCCATGAAGAAAGTCTCTAGTCTTTCAG 6 0.15 No Hit CCGATTTGTTTTTCTGCCGCTATAGTTTGACATGACAGATTGATCAACTG 6 0.15 No Hit AATAAAAAGACCTCAAAATCACCAATGAAGGTTAAGAGGGAGAGCGAGGA 6 0.15 No Hit GCTTTATCTGGTTTTTTCCTAGTCTTCATTTATGCAATGGAATTTCTAGA 5 0.125 No Hit GGACAATCTTCTGGACCCAATGGGAAAGTCTCTCAGCTTCCTGTTCAGGT 5 0.125 No Hit CGAAATATGGCAAATGGGGTTTTGCAAGTTCTGCTTTGTGCAGCTGTATT 5 0.125 No Hit CCTGAAGTCCCGGTTCCAGAGATTTATGTTGATCTTATGGTTCATATGAC 5 0.125 No Hit CTAGTGGCGGTAGTTTGGGTCGTTGGCTTCGACATTTGCAACAAGCAGGG 5 0.125 No Hit GAAGTGTTAGAGCTGGCAGGAAATGCAAGCAAGGATCTAAAGGTGAAACG 5 0.125 No Hit TGTGGATGAATTGAAGGCTGTTGCTGAGGAGTGGAATGTGGAGGCAATGC 5 0.125 No Hit CCAGTTGTCCCTATCATTGTCACTTCTCCCTCCACAACAGCTGCAGCCGC 5 0.125 No Hit GTCGTTAAAGGAGAGAGATCCTTTGCTGAGGATCTTGAGCCGAGGCCTAA 5 0.125 No Hit CCGCAAACAAGGTAGCAACATGGAACCAACCATGAACACCAGTGGTGGGT 5 0.125 No Hit AGCTAATCTCCCACCCCCACTCTCTCTCTCTCTCTCTCTCTCTCTCGTGT 5 0.125 No Hit TTTTTGCCTACTTTAGCTATAGTTCCAGCTGCTAGCATTCTGCACAAGTC 5 0.125 No Hit CAGAGAGACAGAGAGAGAGAGAGAGAGACTGCATTCAACGTATCTGTATA 5 0.125 No Hit TCTCATATAGCGAGTGACAACTGACACGCATAAACATACAGATACATTGA 5 0.125 No Hit GTTGAACGTAGCAGTGGCAAGTTCTTGAGAAGGTTCAAGCTGCCTGAGAA 5 0.125 No Hit AACAGCTAGATAGCCTGCAACCATTAATTTCTAAGGGAATATTGAAAATG 5 0.125 No Hit TGAAATTCGAAAGAGAACTAATGCTGATGTCTGCATCTCAAAAGTGGATA 5 0.125 No Hit GGGGAAAGCCATGAACTTGGCTTCTTCACCAAAAGGGACTAATCCTGCTA 5 0.125 No Hit CAAAGACAGAAAAAGAGAGAGTGAGAGAGAGAGGATGGAGAACTATCGGG 5 0.125 No Hit TGAAAATGGGAAGAAGAAAAGGAACGGCAACCAAACCTTTACTAGACAAA 5 0.125 No Hit GTTTGTACTCTCTTGAAGAGTTGGATCTATCAGGAAACGAGTTCTTCAGT 5 0.125 No Hit GTGAAATTCCAGTAGTGCACTGTGATTTGAAACCAAGTAATGTGCTTTTT 5 0.125 No Hit GGCTCATGCAGCTACTGTTGTGTGTCTTATTGCTGGAGGCTGGTCTATTT 5 0.125 No Hit GGCCTACCTCTCCGGGTCAACAAACAGACCCGACCACAGCAGCCGACCAC 5 0.125 No Hit AGACAACCCAACTGCGTTCCATTCAGCTGTGTTTTATGCTTGTCGGATGT 5 0.125 No Hit GTAGGTTCGTGGTTGCTTCTGGAATTTTCGATGGATATGATGTACCTCAT 5 0.125 No Hit GTTGATGAATTCGAGGACAAGATGAAAGAGCTGGAGAGCATTTGCAATCC 5 0.125 No Hit GAAAGGTAAAAGTCACGCAGGTTATCGCCTCTCATTTCTCTTGAACCTCC 5 0.125 No Hit CTTGTGAAGGCTAAGGCAACTGATACTTCTCCCACGCTTGAAGCTTCAGT 5 0.125 No Hit ACAAGATGGATCCTACAATCAAACTCCATACCCGATCCATCAAATCCAAA 5 0.125 No Hit GTCCAAGTCAAAGCAGTACCGTGGAGTTTTCCAGCAGTGATACACCGTTG 5 0.125 No Hit GTGCTATGCCAGAACAAGATCCATGCTAAATAATCTTGGTCTCCAGAATT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.23750000000000002 0.0 0.0 0.0 0.0 78-79 0.3375 0.0 0.0 0.0 0.0 80-81 0.4375 0.0 0.0 0.0 0.0 82-83 0.475 0.0 0.0 0.0 0.0 84-85 0.5375000000000001 0.0 0.0 0.0 0.0 86-87 0.6125 0.0 0.0 0.0 0.0 88-89 0.8374999999999999 0.0 0.0 0.0 0.0 90-91 1.1375000000000002 0.0 0.0 0.0 0.0 92-93 1.3250000000000002 0.0 0.0 0.0 0.0 94-95 1.6749999999999998 0.0 0.0 0.0 0.0 96-97 2.1375 0.0 0.0 0.0 0.0 98-99 2.3 0.0 0.0 0.0 0.0 100-101 2.4875 0.0 0.0 0.0 0.0 102-103 2.8 0.0 0.0 0.0 0.0 104-105 3.25 0.0 0.0 0.0 0.0 106-107 3.7625 0.0 0.0 0.0 0.0 108-109 4.05 0.0 0.0 0.0 0.0 110-111 4.487500000000001 0.0 0.0 0.0 0.0 112-113 5.075 0.0 0.0 0.0 0.0 114-115 5.475 0.0 0.0 0.0 0.0 116-117 5.9 0.0 0.0 0.0 0.0 118-119 6.2375 0.0 0.0 0.0 0.0 120-121 6.5875 0.0 0.0 0.0 0.0 122-123 6.887499999999999 0.0 0.0 0.0 0.0 124-125 7.475 0.0 0.0 0.0 0.0 126-127 7.95 0.0 0.0 0.0 0.0 128-129 8.6375 0.0 0.0 0.0 0.0 130-131 9.1625 0.0 0.0 0.0 0.0 132-133 9.912500000000001 0.0 0.0 0.0 0.0 134-135 10.8875 0.0 0.0 0.0 0.0 136-137 11.6125 0.0 0.0 0.0 0.0 138-139 12.725 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCCTC 10 0.006830828 145.0 1 AAAAAAA 185 0.0016759153 7.8378377 110-114 >>END_MODULE Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra Read 1098968 spots for SRR26075409.sra Written 1098968 spots for SRR26075409.sra Read 1098957 spots for SRR26075409.sra Written 1098957 spots for SRR26075409.sra SRR ids: ['SRR26075409.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_2ouus18s SRR26075409.sra spots: 21979151 blocks: [[1, 1098957], [1098958, 2197914], [2197915, 3296871], [3296872, 4395828], [4395829, 5494785], [5494786, 6593742], [6593743, 7692699], [7692700, 8791656], [8791657, 9890613], [9890614, 10989570], [10989571, 12088527], [12088528, 13187484], [13187485, 14286441], [14286442, 15385398], [15385399, 16484355], [16484356, 17583312], [17583313, 18682269], [18682270, 19781226], [19781227, 20880183], [20880184, 21979151]] SRR26075409 file size 8112540 SRR26075409 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075409 SRR26075409_1.fastq SRR26075409_2.fastq Input file: SRR26075409_1.fastq Paired file: SRR26075409_2.fastq trimmed: SRR26075409-trimmed-pair1.fastq, SRR26075409-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:40:24 2025 >> started Wed Feb 12 01:40:48 2025 >> done (24.498s) 21979151 read pairs processed; of these: 208 ( 0.00%) short read pairs filtered out after trimming by size control 431038 ( 1.96%) empty read pairs filtered out after trimming by size control 21547905 (98.04%) read pairs available; of these: 3467773 (16.09%) trimmed read pairs available after processing 18080132 (83.91%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 10 0.00% 19 7 0.00% 20 13 0.00% 21 10 0.00% 22 10 0.00% 23 13 0.00% 24 24 0.00% 25 20 0.00% 26 30 0.00% 27 15 0.00% 28 26 0.00% 29 25 0.00% 30 39 0.00% 31 48 0.00% 32 34 0.00% 33 29 0.00% 34 34 0.00% 35 28 0.00% 36 51 0.00% 37 108 0.00% 38 546 0.00% 39 118 0.00% 40 235 0.00% 41 112 0.00% 42 109 0.00% 43 112 0.00% 44 142 0.00% 45 107 0.00% 46 151 0.00% 47 145 0.00% 48 217 0.00% 49 220 0.00% 50 251 0.00% 51 344 0.00% 52 522 0.00% 53 353 0.00% 54 398 0.00% 55 730 0.00% 56 509 0.00% 57 703 0.00% 58 1205 0.01% 59 783 0.00% 60 953 0.00% 61 891 0.00% 62 1064 0.00% 63 1661 0.01% 64 1412 0.01% 65 1453 0.01% 66 1442 0.01% 67 1624 0.01% 68 1835 0.01% 69 2099 0.01% 70 2329 0.01% 71 2690 0.01% 72 3015 0.01% 73 3450 0.02% 74 3787 0.02% 75 4174 0.02% 76 4450 0.02% 77 4829 0.02% 78 5199 0.02% 79 6157 0.03% 80 6472 0.03% 81 7791 0.04% 82 8358 0.04% 83 9660 0.04% 84 10295 0.05% 85 11219 0.05% 86 11820 0.05% 87 12447 0.06% 88 13283 0.06% 89 13911 0.06% 90 15344 0.07% 91 16978 0.08% 92 18262 0.08% 93 19922 0.09% 94 21384 0.10% 95 22488 0.10% 96 23425 0.11% 97 24252 0.11% 98 25332 0.12% 99 26616 0.12% 100 27397 0.13% 101 29031 0.13% 102 30976 0.14% 103 32996 0.15% 104 34561 0.16% 105 35356 0.16% 106 37473 0.17% 107 39006 0.18% 108 38642 0.18% 109 40115 0.19% 110 40403 0.19% 111 42013 0.19% 112 43977 0.20% 113 44823 0.21% 114 46782 0.22% 115 49390 0.23% 116 51147 0.24% 117 50830 0.24% 118 51903 0.24% 119 53110 0.25% 120 54380 0.25% 121 55042 0.26% 122 56143 0.26% 123 58015 0.27% 124 59618 0.28% 125 61418 0.29% 126 63094 0.29% 127 64966 0.30% 128 65304 0.30% 129 66548 0.31% 130 67172 0.31% 131 67870 0.31% 132 68725 0.32% 133 69395 0.32% 134 71688 0.33% 135 74350 0.35% 136 75618 0.35% 137 76093 0.35% 138 79294 0.37% 139 78897 0.37% 140 79221 0.37% 141 80242 0.37% 142 81199 0.38% 143 81699 0.38% 144 82056 0.38% 145 84261 0.39% 146 85571 0.40% 147 87835 0.41% 148 89285 0.41% 149 89954 0.42% 150 90530 0.42% 151 18080132 83.91% 21547905 reads passed initial QC criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=4.77 fanout-score-rank=21 prefix-density=0.35 prefix-fanout=4.0 sequence=GCATTCTCAGGCAGCCTAAACCTCCTC criterion=fanout-score sequence-density=0.14 sequence-density-rank=20 fanout-score=28.37 fanout-score-rank=1 prefix-density=0.38 prefix-fanout=10.1 sequence=AGCATCATCATC criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=41 prefix-density=0.23 prefix-fanout=2.0 sequence=CCAGACCAGCAGAGGTTGAT criterion=fanout-score sequence-density=0.02 sequence-density-rank=40 fanout-score=380.04 fanout-score-rank=1 prefix-density=0.43 prefix-fanout=16.9 sequence=GAGAAGAAACACTAAAAAAATCACACAAGCGAAATCTCCAGCGAGAAGAGAAAGATGGATTTCAGAATCATGGGTCTTGACGCGCCACTCTTCAACACCCTCCAGCACATGATGGATGCAAGTGATCATGAGGCAGACAAGTCCTTCAATGCGCCAACACGCACTTACGTACGTGATGCCAAGGCAATGGCATCAACACCAGCTGATGTGAAAGAGTATCCAA SRR26075409 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:41:28 Started mapping on | Feb 12 01:41:29 Finished on | Feb 12 01:44:26 Mapping speed, Million of reads per hour | 438.26 Number of input reads | 21547905 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 19178020 Uniquely mapped reads % | 89.00% Average mapped length | 291.68 Number of splices: Total | 14887558 Number of splices: Annotated (sjdb) | 14472028 Number of splices: GT/AG | 14639132 Number of splices: GC/AG | 174601 Number of splices: AT/AC | 21013 Number of splices: Non-canonical | 52812 Mismatch rate per base, % | 0.42% Deletion rate per base | 0.03% Deletion average length | 2.98 Insertion rate per base | 0.02% Insertion average length | 2.64 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 646876 % of reads mapped to multiple loci | 3.00% Number of reads mapped to too many loci | 40331 % of reads mapped to too many loci | 0.19% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.96% % of reads unmapped: other | 0.85% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1723009 1723009 1723009 N_multimapping 646876 646876 646876 N_noFeature 490770 18929561 654014 N_ambiguous 217771 1580 131691 UnstrandedReadsAssigned:18469479 PositiveStrandReadsAssigned:246879 NegativeStrandReadsAssigned:18392315 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075409 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075409-trimmed-pair1.fastq SRR26075409-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 21,547,905 reads, 18,791,160 reads pseudoaligned [quant] estimated average fragment length: 211.996 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,190 rounds 52401 SRR26075409.ke.tsv 34699 SRR26075409.se.tsv 87100 total ==> SRR26075409.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1807 3378 86.6003 Potri.005G024800.1.v4.1 1035 824.004 4790 269.293 Potri.004G059700.1.v4.1 961 750.02 22 1.35884 Potri.007G009000.2.v4.1 1416 1205 0 0 Potri.003G141000.2.v4.1 2943 2732 656 11.1235 Potri.016G087400.1.v4.1 270 91.0661 1416.85 720.752 Potri.015G069301.1.v4.1 564 354.919 0 0 Potri.010G195200.1.v4.1 1773 1562 43 1.27528 Potri.012G127500.1.v4.1 977 766.015 2090 126.394 ==> SRR26075409.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 204 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 190 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 5 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 311 SRR26075409 completed mapping pipeline successfully