Starting /dee2/code/volunteer_pipeline.sh SRR3207965 current disk space = 2796418781184 free memory = 1575039976 SRR3207965_1.fastq is conventional basespace SRR3207965_1.fastq read1 length is 100 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3207965_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11073869 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0 30.0 30.0 30.0 30.0 30.0 2 30.0 30.0 30.0 30.0 30.0 30.0 3 30.0 30.0 30.0 30.0 30.0 30.0 4 30.0 30.0 30.0 30.0 30.0 30.0 5 30.0 30.0 30.0 30.0 30.0 30.0 6 30.0 30.0 30.0 30.0 30.0 30.0 7 30.0 30.0 30.0 30.0 30.0 30.0 8 30.0 30.0 30.0 30.0 30.0 30.0 9 30.0 30.0 30.0 30.0 30.0 30.0 10-11 30.0 30.0 30.0 30.0 30.0 30.0 12-13 30.0 30.0 30.0 30.0 30.0 30.0 14-15 30.0 30.0 30.0 30.0 30.0 30.0 16-17 30.0 30.0 30.0 30.0 30.0 30.0 18-19 30.0 30.0 30.0 30.0 30.0 30.0 20-21 30.0 30.0 30.0 30.0 30.0 30.0 22-23 30.0 30.0 30.0 30.0 30.0 30.0 24-25 30.0 30.0 30.0 30.0 30.0 30.0 26-27 30.0 30.0 30.0 30.0 30.0 30.0 28-29 30.0 30.0 30.0 30.0 30.0 30.0 30-31 30.0 30.0 30.0 30.0 30.0 30.0 32-33 30.0 30.0 30.0 30.0 30.0 30.0 34-35 30.0 30.0 30.0 30.0 30.0 30.0 36-37 30.0 30.0 30.0 30.0 30.0 30.0 38-39 30.0 30.0 30.0 30.0 30.0 30.0 40-41 30.0 30.0 30.0 30.0 30.0 30.0 42-43 30.0 30.0 30.0 30.0 30.0 30.0 44-45 30.0 30.0 30.0 30.0 30.0 30.0 46-47 30.0 30.0 30.0 30.0 30.0 30.0 48-49 30.0 30.0 30.0 30.0 30.0 30.0 50-51 30.0 30.0 30.0 30.0 30.0 30.0 52-53 30.0 30.0 30.0 30.0 30.0 30.0 54-55 30.0 30.0 30.0 30.0 30.0 30.0 56-57 30.0 30.0 30.0 30.0 30.0 30.0 58-59 30.0 30.0 30.0 30.0 30.0 30.0 60-61 30.0 30.0 30.0 30.0 30.0 30.0 62-63 30.0 30.0 30.0 30.0 30.0 30.0 64-65 30.0 30.0 30.0 30.0 30.0 30.0 66-67 30.0 30.0 30.0 30.0 30.0 30.0 68-69 30.0 30.0 30.0 30.0 30.0 30.0 70-71 30.0 30.0 30.0 30.0 30.0 30.0 72-73 30.0 30.0 30.0 30.0 30.0 30.0 74-75 30.0 30.0 30.0 30.0 30.0 30.0 76-77 30.0 30.0 30.0 30.0 30.0 30.0 78-79 30.0 30.0 30.0 30.0 30.0 30.0 80-81 30.0 30.0 30.0 30.0 30.0 30.0 82-83 30.0 30.0 30.0 30.0 30.0 30.0 84-85 30.0 30.0 30.0 30.0 30.0 30.0 86-87 30.0 30.0 30.0 30.0 30.0 30.0 88-89 30.0 30.0 30.0 30.0 30.0 30.0 90-91 30.0 30.0 30.0 30.0 30.0 30.0 92-93 30.0 30.0 30.0 30.0 30.0 30.0 94-95 30.0 30.0 30.0 30.0 30.0 30.0 96-97 30.0 30.0 30.0 30.0 30.0 30.0 98-99 30.0 30.0 30.0 30.0 30.0 30.0 100 30.0 30.0 30.0 30.0 30.0 30.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-11 0.0 1101 12-13 0.0 1101 14-15 0.0 1101 16-17 0.0 1101 18-19 0.0 1101 20-21 0.0 1101 22-23 0.0 1101 24-25 0.0 1101 26-27 0.0 1101 28-29 0.0 1101 30-31 0.0 1101 32-33 0.0 1101 34-35 0.0 1101 36-37 0.0 1101 38-39 0.0 1101 40-41 0.0 1101 42-43 0.0 1101 44-45 0.0 1101 46-47 0.0 1101 48-49 0.0 1101 50-51 0.0 1101 52-53 0.0 1101 54-55 0.0 1101 56-57 0.0 1101 58-59 0.0 1101 60-61 0.0 1101 62-63 0.0 1101 64-65 0.0 1101 66-67 0.0 1101 68-69 0.0 1101 70-71 0.0 1101 72-73 0.0 1101 74-75 0.0 1101 76-77 0.0 1101 78-79 0.0 1101 80-81 0.0 1101 82-83 0.0 1101 84-85 0.0 1101 86-87 0.0 1101 88-89 0.0 1101 90-91 0.0 1101 92-93 0.0 1101 94-95 0.0 1101 96-97 0.0 1101 98-99 0.0 1101 100 0.0 1102 1 0.0 1102 2 0.0 1102 3 0.0 1102 4 0.0 1102 5 0.0 1102 6 0.0 1102 7 0.0 1102 8 0.0 1102 9 0.0 1102 10-11 0.0 1102 12-13 0.0 1102 14-15 0.0 1102 16-17 0.0 1102 18-19 0.0 1102 20-21 0.0 1102 22-23 0.0 1102 24-25 0.0 1102 26-27 0.0 1102 28-29 0.0 1102 30-31 0.0 1102 32-33 0.0 1102 34-35 0.0 1102 36-37 0.0 1102 38-39 0.0 1102 40-41 0.0 1102 42-43 0.0 1102 44-45 0.0 1102 46-47 0.0 1102 48-49 0.0 1102 50-51 0.0 1102 52-53 0.0 1102 54-55 0.0 1102 56-57 0.0 1102 58-59 0.0 1102 60-61 0.0 1102 62-63 0.0 1102 64-65 0.0 1102 66-67 0.0 1102 68-69 0.0 1102 70-71 0.0 1102 72-73 0.0 1102 74-75 0.0 1102 76-77 0.0 1102 78-79 0.0 1102 80-81 0.0 1102 82-83 0.0 1102 84-85 0.0 1102 86-87 0.0 1102 88-89 0.0 1102 90-91 0.0 1102 92-93 0.0 1102 94-95 0.0 1102 96-97 0.0 1102 98-99 0.0 1102 100 0.0 1103 1 0.0 1103 2 0.0 1103 3 0.0 1103 4 0.0 1103 5 0.0 1103 6 0.0 1103 7 0.0 1103 8 0.0 1103 9 0.0 1103 10-11 0.0 1103 12-13 0.0 1103 14-15 0.0 1103 16-17 0.0 1103 18-19 0.0 1103 20-21 0.0 1103 22-23 0.0 1103 24-25 0.0 1103 26-27 0.0 1103 28-29 0.0 1103 30-31 0.0 1103 32-33 0.0 1103 34-35 0.0 1103 36-37 0.0 1103 38-39 0.0 1103 40-41 0.0 1103 42-43 0.0 1103 44-45 0.0 1103 46-47 0.0 1103 48-49 0.0 1103 50-51 0.0 1103 52-53 0.0 1103 54-55 0.0 1103 56-57 0.0 1103 58-59 0.0 1103 60-61 0.0 1103 62-63 0.0 1103 64-65 0.0 1103 66-67 0.0 1103 68-69 0.0 1103 70-71 0.0 1103 72-73 0.0 1103 74-75 0.0 1103 76-77 0.0 1103 78-79 0.0 1103 80-81 0.0 1103 82-83 0.0 1103 84-85 0.0 1103 86-87 0.0 1103 88-89 0.0 1103 90-91 0.0 1103 92-93 0.0 1103 94-95 0.0 1103 96-97 0.0 1103 98-99 0.0 1103 100 0.0 1104 1 0.0 1104 2 0.0 1104 3 0.0 1104 4 0.0 1104 5 0.0 1104 6 0.0 1104 7 0.0 1104 8 0.0 1104 9 0.0 1104 10-11 0.0 1104 12-13 0.0 1104 14-15 0.0 1104 16-17 0.0 1104 18-19 0.0 1104 20-21 0.0 1104 22-23 0.0 1104 24-25 0.0 1104 26-27 0.0 1104 28-29 0.0 1104 30-31 0.0 1104 32-33 0.0 1104 34-35 0.0 1104 36-37 0.0 1104 38-39 0.0 1104 40-41 0.0 1104 42-43 0.0 1104 44-45 0.0 1104 46-47 0.0 1104 48-49 0.0 1104 50-51 0.0 1104 52-53 0.0 1104 54-55 0.0 1104 56-57 0.0 1104 58-59 0.0 1104 60-61 0.0 1104 62-63 0.0 1104 64-65 0.0 1104 66-67 0.0 1104 68-69 0.0 1104 70-71 0.0 1104 72-73 0.0 1104 74-75 0.0 1104 76-77 0.0 1104 78-79 0.0 1104 80-81 0.0 1104 82-83 0.0 1104 84-85 0.0 1104 86-87 0.0 1104 88-89 0.0 1104 90-91 0.0 1104 92-93 0.0 1104 94-95 0.0 1104 96-97 0.0 1104 98-99 0.0 1104 100 0.0 1105 1 0.0 1105 2 0.0 1105 3 0.0 1105 4 0.0 1105 5 0.0 1105 6 0.0 1105 7 0.0 1105 8 0.0 1105 9 0.0 1105 10-11 0.0 1105 12-13 0.0 1105 14-15 0.0 1105 16-17 0.0 1105 18-19 0.0 1105 20-21 0.0 1105 22-23 0.0 1105 24-25 0.0 1105 26-27 0.0 1105 28-29 0.0 1105 30-31 0.0 1105 32-33 0.0 1105 34-35 0.0 1105 36-37 0.0 1105 38-39 0.0 1105 40-41 0.0 1105 42-43 0.0 1105 44-45 0.0 1105 46-47 0.0 1105 48-49 0.0 1105 50-51 0.0 1105 52-53 0.0 1105 54-55 0.0 1105 56-57 0.0 1105 58-59 0.0 1105 60-61 0.0 1105 62-63 0.0 1105 64-65 0.0 1105 66-67 0.0 1105 68-69 0.0 1105 70-71 0.0 1105 72-73 0.0 1105 74-75 0.0 1105 76-77 0.0 1105 78-79 0.0 1105 80-81 0.0 1105 82-83 0.0 1105 84-85 0.0 1105 86-87 0.0 1105 88-89 0.0 1105 90-91 0.0 1105 92-93 0.0 1105 94-95 0.0 1105 96-97 0.0 1105 98-99 0.0 1105 100 0.0 1106 1 0.0 1106 2 0.0 1106 3 0.0 1106 4 0.0 1106 5 0.0 1106 6 0.0 1106 7 0.0 1106 8 0.0 1106 9 0.0 1106 10-11 0.0 1106 12-13 0.0 1106 14-15 0.0 1106 16-17 0.0 1106 18-19 0.0 1106 20-21 0.0 1106 22-23 0.0 1106 24-25 0.0 1106 26-27 0.0 1106 28-29 0.0 1106 30-31 0.0 1106 32-33 0.0 1106 34-35 0.0 1106 36-37 0.0 1106 38-39 0.0 1106 40-41 0.0 1106 42-43 0.0 1106 44-45 0.0 1106 46-47 0.0 1106 48-49 0.0 1106 50-51 0.0 1106 52-53 0.0 1106 54-55 0.0 1106 56-57 0.0 1106 58-59 0.0 1106 60-61 0.0 1106 62-63 0.0 1106 64-65 0.0 1106 66-67 0.0 1106 68-69 0.0 1106 70-71 0.0 1106 72-73 0.0 1106 74-75 0.0 1106 76-77 0.0 1106 78-79 0.0 1106 80-81 0.0 1106 82-83 0.0 1106 84-85 0.0 1106 86-87 0.0 1106 88-89 0.0 1106 90-91 0.0 1106 92-93 0.0 1106 94-95 0.0 1106 96-97 0.0 1106 98-99 0.0 1106 100 0.0 1107 1 0.0 1107 2 0.0 1107 3 0.0 1107 4 0.0 1107 5 0.0 1107 6 0.0 1107 7 0.0 1107 8 0.0 1107 9 0.0 1107 10-11 0.0 1107 12-13 0.0 1107 14-15 0.0 1107 16-17 0.0 1107 18-19 0.0 1107 20-21 0.0 1107 22-23 0.0 1107 24-25 0.0 1107 26-27 0.0 1107 28-29 0.0 1107 30-31 0.0 1107 32-33 0.0 1107 34-35 0.0 1107 36-37 0.0 1107 38-39 0.0 1107 40-41 0.0 1107 42-43 0.0 1107 44-45 0.0 1107 46-47 0.0 1107 48-49 0.0 1107 50-51 0.0 1107 52-53 0.0 1107 54-55 0.0 1107 56-57 0.0 1107 58-59 0.0 1107 60-61 0.0 1107 62-63 0.0 1107 64-65 0.0 1107 66-67 0.0 1107 68-69 0.0 1107 70-71 0.0 1107 72-73 0.0 1107 74-75 0.0 1107 76-77 0.0 1107 78-79 0.0 1107 80-81 0.0 1107 82-83 0.0 1107 84-85 0.0 1107 86-87 0.0 1107 88-89 0.0 1107 90-91 0.0 1107 92-93 0.0 1107 94-95 0.0 1107 96-97 0.0 1107 98-99 0.0 1107 100 0.0 1108 1 0.0 1108 2 0.0 1108 3 0.0 1108 4 0.0 1108 5 0.0 1108 6 0.0 1108 7 0.0 1108 8 0.0 1108 9 0.0 1108 10-11 0.0 1108 12-13 0.0 1108 14-15 0.0 1108 16-17 0.0 1108 18-19 0.0 1108 20-21 0.0 1108 22-23 0.0 1108 24-25 0.0 1108 26-27 0.0 1108 28-29 0.0 1108 30-31 0.0 1108 32-33 0.0 1108 34-35 0.0 1108 36-37 0.0 1108 38-39 0.0 1108 40-41 0.0 1108 42-43 0.0 1108 44-45 0.0 1108 46-47 0.0 1108 48-49 0.0 1108 50-51 0.0 1108 52-53 0.0 1108 54-55 0.0 1108 56-57 0.0 1108 58-59 0.0 1108 60-61 0.0 1108 62-63 0.0 1108 64-65 0.0 1108 66-67 0.0 1108 68-69 0.0 1108 70-71 0.0 1108 72-73 0.0 1108 74-75 0.0 1108 76-77 0.0 1108 78-79 0.0 1108 80-81 0.0 1108 82-83 0.0 1108 84-85 0.0 1108 86-87 0.0 1108 88-89 0.0 1108 90-91 0.0 1108 92-93 0.0 1108 94-95 0.0 1108 96-97 0.0 1108 98-99 0.0 1108 100 0.0 1109 1 0.0 1109 2 0.0 1109 3 0.0 1109 4 0.0 1109 5 0.0 1109 6 0.0 1109 7 0.0 1109 8 0.0 1109 9 0.0 1109 10-11 0.0 1109 12-13 0.0 1109 14-15 0.0 1109 16-17 0.0 1109 18-19 0.0 1109 20-21 0.0 1109 22-23 0.0 1109 24-25 0.0 1109 26-27 0.0 1109 28-29 0.0 1109 30-31 0.0 1109 32-33 0.0 1109 34-35 0.0 1109 36-37 0.0 1109 38-39 0.0 1109 40-41 0.0 1109 42-43 0.0 1109 44-45 0.0 1109 46-47 0.0 1109 48-49 0.0 1109 50-51 0.0 1109 52-53 0.0 1109 54-55 0.0 1109 56-57 0.0 1109 58-59 0.0 1109 60-61 0.0 1109 62-63 0.0 1109 64-65 0.0 1109 66-67 0.0 1109 68-69 0.0 1109 70-71 0.0 1109 72-73 0.0 1109 74-75 0.0 1109 76-77 0.0 1109 78-79 0.0 1109 80-81 0.0 1109 82-83 0.0 1109 84-85 0.0 1109 86-87 0.0 1109 88-89 0.0 1109 90-91 0.0 1109 92-93 0.0 1109 94-95 0.0 1109 96-97 0.0 1109 98-99 0.0 1109 100 0.0 1110 1 0.0 1110 2 0.0 1110 3 0.0 1110 4 0.0 1110 5 0.0 1110 6 0.0 1110 7 0.0 1110 8 0.0 1110 9 0.0 1110 10-11 0.0 1110 12-13 0.0 1110 14-15 0.0 1110 16-17 0.0 1110 18-19 0.0 1110 20-21 0.0 1110 22-23 0.0 1110 24-25 0.0 1110 26-27 0.0 1110 28-29 0.0 1110 30-31 0.0 1110 32-33 0.0 1110 34-35 0.0 1110 36-37 0.0 1110 38-39 0.0 1110 40-41 0.0 1110 42-43 0.0 1110 44-45 0.0 1110 46-47 0.0 1110 48-49 0.0 1110 50-51 0.0 1110 52-53 0.0 1110 54-55 0.0 1110 56-57 0.0 1110 58-59 0.0 1110 60-61 0.0 1110 62-63 0.0 1110 64-65 0.0 1110 66-67 0.0 1110 68-69 0.0 1110 70-71 0.0 1110 72-73 0.0 1110 74-75 0.0 1110 76-77 0.0 1110 78-79 0.0 1110 80-81 0.0 1110 82-83 0.0 1110 84-85 0.0 1110 86-87 0.0 1110 88-89 0.0 1110 90-91 0.0 1110 92-93 0.0 1110 94-95 0.0 1110 96-97 0.0 1110 98-99 0.0 1110 100 0.0 1111 1 0.0 1111 2 0.0 1111 3 0.0 1111 4 0.0 1111 5 0.0 1111 6 0.0 1111 7 0.0 1111 8 0.0 1111 9 0.0 1111 10-11 0.0 1111 12-13 0.0 1111 14-15 0.0 1111 16-17 0.0 1111 18-19 0.0 1111 20-21 0.0 1111 22-23 0.0 1111 24-25 0.0 1111 26-27 0.0 1111 28-29 0.0 1111 30-31 0.0 1111 32-33 0.0 1111 34-35 0.0 1111 36-37 0.0 1111 38-39 0.0 1111 40-41 0.0 1111 42-43 0.0 1111 44-45 0.0 1111 46-47 0.0 1111 48-49 0.0 1111 50-51 0.0 1111 52-53 0.0 1111 54-55 0.0 1111 56-57 0.0 1111 58-59 0.0 1111 60-61 0.0 1111 62-63 0.0 1111 64-65 0.0 1111 66-67 0.0 1111 68-69 0.0 1111 70-71 0.0 1111 72-73 0.0 1111 74-75 0.0 1111 76-77 0.0 1111 78-79 0.0 1111 80-81 0.0 1111 82-83 0.0 1111 84-85 0.0 1111 86-87 0.0 1111 88-89 0.0 1111 90-91 0.0 1111 92-93 0.0 1111 94-95 0.0 1111 96-97 0.0 1111 98-99 0.0 1111 100 0.0 1112 1 0.0 1112 2 0.0 1112 3 0.0 1112 4 0.0 1112 5 0.0 1112 6 0.0 1112 7 0.0 1112 8 0.0 1112 9 0.0 1112 10-11 0.0 1112 12-13 0.0 1112 14-15 0.0 1112 16-17 0.0 1112 18-19 0.0 1112 20-21 0.0 1112 22-23 0.0 1112 24-25 0.0 1112 26-27 0.0 1112 28-29 0.0 1112 30-31 0.0 1112 32-33 0.0 1112 34-35 0.0 1112 36-37 0.0 1112 38-39 0.0 1112 40-41 0.0 1112 42-43 0.0 1112 44-45 0.0 1112 46-47 0.0 1112 48-49 0.0 1112 50-51 0.0 1112 52-53 0.0 1112 54-55 0.0 1112 56-57 0.0 1112 58-59 0.0 1112 60-61 0.0 1112 62-63 0.0 1112 64-65 0.0 1112 66-67 0.0 1112 68-69 0.0 1112 70-71 0.0 1112 72-73 0.0 1112 74-75 0.0 1112 76-77 0.0 1112 78-79 0.0 1112 80-81 0.0 1112 82-83 0.0 1112 84-85 0.0 1112 86-87 0.0 1112 88-89 0.0 1112 90-91 0.0 1112 92-93 0.0 1112 94-95 0.0 1112 96-97 0.0 1112 98-99 0.0 1112 100 0.0 1113 1 0.0 1113 2 0.0 1113 3 0.0 1113 4 0.0 1113 5 0.0 1113 6 0.0 1113 7 0.0 1113 8 0.0 1113 9 0.0 1113 10-11 0.0 1113 12-13 0.0 1113 14-15 0.0 1113 16-17 0.0 1113 18-19 0.0 1113 20-21 0.0 1113 22-23 0.0 1113 24-25 0.0 1113 26-27 0.0 1113 28-29 0.0 1113 30-31 0.0 1113 32-33 0.0 1113 34-35 0.0 1113 36-37 0.0 1113 38-39 0.0 1113 40-41 0.0 1113 42-43 0.0 1113 44-45 0.0 1113 46-47 0.0 1113 48-49 0.0 1113 50-51 0.0 1113 52-53 0.0 1113 54-55 0.0 1113 56-57 0.0 1113 58-59 0.0 1113 60-61 0.0 1113 62-63 0.0 1113 64-65 0.0 1113 66-67 0.0 1113 68-69 0.0 1113 70-71 0.0 1113 72-73 0.0 1113 74-75 0.0 1113 76-77 0.0 1113 78-79 0.0 1113 80-81 0.0 1113 82-83 0.0 1113 84-85 0.0 1113 86-87 0.0 1113 88-89 0.0 1113 90-91 0.0 1113 92-93 0.0 1113 94-95 0.0 1113 96-97 0.0 1113 98-99 0.0 1113 100 0.0 1114 1 0.0 1114 2 0.0 1114 3 0.0 1114 4 0.0 1114 5 0.0 1114 6 0.0 1114 7 0.0 1114 8 0.0 1114 9 0.0 1114 10-11 0.0 1114 12-13 0.0 1114 14-15 0.0 1114 16-17 0.0 1114 18-19 0.0 1114 20-21 0.0 1114 22-23 0.0 1114 24-25 0.0 1114 26-27 0.0 1114 28-29 0.0 1114 30-31 0.0 1114 32-33 0.0 1114 34-35 0.0 1114 36-37 0.0 1114 38-39 0.0 1114 40-41 0.0 1114 42-43 0.0 1114 44-45 0.0 1114 46-47 0.0 1114 48-49 0.0 1114 50-51 0.0 1114 52-53 0.0 1114 54-55 0.0 1114 56-57 0.0 1114 58-59 0.0 1114 60-61 0.0 1114 62-63 0.0 1114 64-65 0.0 1114 66-67 0.0 1114 68-69 0.0 1114 70-71 0.0 1114 72-73 0.0 1114 74-75 0.0 1114 76-77 0.0 1114 78-79 0.0 1114 80-81 0.0 1114 82-83 0.0 1114 84-85 0.0 1114 86-87 0.0 1114 88-89 0.0 1114 90-91 0.0 1114 92-93 0.0 1114 94-95 0.0 1114 96-97 0.0 1114 98-99 0.0 1114 100 0.0 1115 1 0.0 1115 2 0.0 1115 3 0.0 1115 4 0.0 1115 5 0.0 1115 6 0.0 1115 7 0.0 1115 8 0.0 1115 9 0.0 1115 10-11 0.0 1115 12-13 0.0 1115 14-15 0.0 1115 16-17 0.0 1115 18-19 0.0 1115 20-21 0.0 1115 22-23 0.0 1115 24-25 0.0 1115 26-27 0.0 1115 28-29 0.0 1115 30-31 0.0 1115 32-33 0.0 1115 34-35 0.0 1115 36-37 0.0 1115 38-39 0.0 1115 40-41 0.0 1115 42-43 0.0 1115 44-45 0.0 1115 46-47 0.0 1115 48-49 0.0 1115 50-51 0.0 1115 52-53 0.0 1115 54-55 0.0 1115 56-57 0.0 1115 58-59 0.0 1115 60-61 0.0 1115 62-63 0.0 1115 64-65 0.0 1115 66-67 0.0 1115 68-69 0.0 1115 70-71 0.0 1115 72-73 0.0 1115 74-75 0.0 1115 76-77 0.0 1115 78-79 0.0 1115 80-81 0.0 1115 82-83 0.0 1115 84-85 0.0 1115 86-87 0.0 1115 88-89 0.0 1115 90-91 0.0 1115 92-93 0.0 1115 94-95 0.0 1115 96-97 0.0 1115 98-99 0.0 1115 100 0.0 1116 1 0.0 1116 2 0.0 1116 3 0.0 1116 4 0.0 1116 5 0.0 1116 6 0.0 1116 7 0.0 1116 8 0.0 1116 9 0.0 1116 10-11 0.0 1116 12-13 0.0 1116 14-15 0.0 1116 16-17 0.0 1116 18-19 0.0 1116 20-21 0.0 1116 22-23 0.0 1116 24-25 0.0 1116 26-27 0.0 1116 28-29 0.0 1116 30-31 0.0 1116 32-33 0.0 1116 34-35 0.0 1116 36-37 0.0 1116 38-39 0.0 1116 40-41 0.0 1116 42-43 0.0 1116 44-45 0.0 1116 46-47 0.0 1116 48-49 0.0 1116 50-51 0.0 1116 52-53 0.0 1116 54-55 0.0 1116 56-57 0.0 1116 58-59 0.0 1116 60-61 0.0 1116 62-63 0.0 1116 64-65 0.0 1116 66-67 0.0 1116 68-69 0.0 1116 70-71 0.0 1116 72-73 0.0 1116 74-75 0.0 1116 76-77 0.0 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2315 46-47 0.0 2315 48-49 0.0 2315 50-51 0.0 2315 52-53 0.0 2315 54-55 0.0 2315 56-57 0.0 2315 58-59 0.0 2315 60-61 0.0 2315 62-63 0.0 2315 64-65 0.0 2315 66-67 0.0 2315 68-69 0.0 2315 70-71 0.0 2315 72-73 0.0 2315 74-75 0.0 2315 76-77 0.0 2315 78-79 0.0 2315 80-81 0.0 2315 82-83 0.0 2315 84-85 0.0 2315 86-87 0.0 2315 88-89 0.0 2315 90-91 0.0 2315 92-93 0.0 2315 94-95 0.0 2315 96-97 0.0 2315 98-99 0.0 2315 100 0.0 2316 1 0.0 2316 2 0.0 2316 3 0.0 2316 4 0.0 2316 5 0.0 2316 6 0.0 2316 7 0.0 2316 8 0.0 2316 9 0.0 2316 10-11 0.0 2316 12-13 0.0 2316 14-15 0.0 2316 16-17 0.0 2316 18-19 0.0 2316 20-21 0.0 2316 22-23 0.0 2316 24-25 0.0 2316 26-27 0.0 2316 28-29 0.0 2316 30-31 0.0 2316 32-33 0.0 2316 34-35 0.0 2316 36-37 0.0 2316 38-39 0.0 2316 40-41 0.0 2316 42-43 0.0 2316 44-45 0.0 2316 46-47 0.0 2316 48-49 0.0 2316 50-51 0.0 2316 52-53 0.0 2316 54-55 0.0 2316 56-57 0.0 2316 58-59 0.0 2316 60-61 0.0 2316 62-63 0.0 2316 64-65 0.0 2316 66-67 0.0 2316 68-69 0.0 2316 70-71 0.0 2316 72-73 0.0 2316 74-75 0.0 2316 76-77 0.0 2316 78-79 0.0 2316 80-81 0.0 2316 82-83 0.0 2316 84-85 0.0 2316 86-87 0.0 2316 88-89 0.0 2316 90-91 0.0 2316 92-93 0.0 2316 94-95 0.0 2316 96-97 0.0 2316 98-99 0.0 2316 100 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 1.1073869E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.686458856546057 16.304203408035878 16.73067982872298 41.27865790669508 2 19.724316767698806 24.83595390192895 37.08479845661891 18.35493087375334 3 21.409229240475934 27.67771589134746 28.608962233524704 22.3040926346519 4 23.233867043216783 32.93837050086108 21.14872408189044 22.679038374031695 5 24.334437516707247 35.33979828484319 22.41193926871031 17.91382492973926 6 19.065976803123117 36.93476769032107 24.408530030927544 19.590725475628272 7 16.795638453010415 18.530605698875434 43.74789876961702 20.92585707849714 8 18.910671599962036 24.151197743083287 29.51276559258557 27.4253650643691 9 20.485053597798565 23.390849214488632 31.18833173843758 24.935765449275227 10-11 22.76768851067319 33.6366720610475 22.527546605436637 21.068092822842676 12-13 20.114252751229042 26.663612329168785 29.94368996057295 23.278444959029223 14-15 20.622101453430595 28.109760012512343 28.829531033823862 22.438607500233207 16-17 21.91541637344635 28.085373774965188 27.608182831131558 22.391027020456896 18-19 21.428752678941752 28.14410663517873 28.114830507747563 22.312310178131963 20-21 21.837331649850654 28.10744374888307 28.17479599948311 21.88042860178317 22-23 21.38306619293438 29.53528000639349 27.556490498037476 21.52516330263465 24-25 21.10406910232065 28.58768705161828 28.104681314147296 22.203562531913782 26-27 21.2071995794785 28.36396655947438 27.826643063955338 22.602190797091783 28-29 21.783773677612352 28.76280298131081 27.708252797236906 21.745170543839937 30-31 21.260329964603365 28.170395883647455 28.433574431694463 22.13569972005472 32-33 21.328886949990107 28.863841535420004 27.669006198285352 22.138265316304537 34-35 21.36736943519921 28.18857618778044 28.07402724377541 22.37002713324494 36-37 21.820634685131278 28.139343169040558 28.397504973194103 21.64251717263406 38-39 21.55243573858423 28.674242037719612 28.210948675661594 21.562373548034568 40-41 21.463347633966052 28.96904866763369 28.118889613016012 21.44871408538425 42-43 21.215430668359904 28.056802008403746 28.477431871372143 22.250335451864203 44-45 21.786739575842915 28.094255043110948 27.990452117502922 22.12855326354321 46-47 21.64453995256762 28.5432715521558 28.279565163720104 21.53262333155648 48-49 21.78984147274995 28.213662271063527 28.368165633890012 21.628330622296506 50-51 21.578935058740537 28.182385939367716 27.70270264168738 22.53597636020437 52-53 22.079356365873572 28.19912354029111 28.12475025666278 21.596769837172538 54-55 21.31608654572309 27.999861656300972 28.482394003396642 22.201657794579294 56-57 21.427064018907934 28.041053221778224 28.59852324422476 21.93335951508908 58-59 21.96487966400903 28.119088278902343 28.38871852285773 21.5273135342309 60-61 21.471176875941012 28.057298673119575 28.352461998602298 22.11906245233712 62-63 21.944538986328986 28.053763323369633 28.634960373831404 21.366737316469973 64-65 21.606057467358518 29.168676277460026 27.858519908443924 21.366746346737532 66-67 21.37316686697305 29.246463002226232 27.88807145903568 21.492298671765038 68-69 21.53535498749353 29.266275409253982 27.82103526780026 21.377334335452225 70-71 21.50965484601633 29.26781055473927 27.793732253831067 21.428802345413335 72-73 21.45677811431578 29.038225032280952 27.989512969676632 21.515483883726635 74-75 21.54571270438543 28.904703496131294 28.06534464151599 21.484239157967284 76-77 21.705110472229716 28.864080837510357 27.93320022116931 21.49760846909061 78-79 21.587098420615234 28.70894084082086 28.050390518435787 21.653570220128124 80-81 21.702816784269345 28.729615638400634 28.007790231219097 21.55977734611092 82-83 21.72144171111289 28.760444971852205 27.991490598272385 21.52662271876252 84-85 21.612170958496982 28.551416853495375 28.185248534184392 21.65116365382325 86-87 21.756483664381438 28.620922822908597 28.10189916460092 21.52069434810905 88-89 21.854096341576735 28.57170786470384 28.015217626287615 21.558978167431817 90-91 21.72890974238543 28.494964135840874 28.132109924724592 21.64401619704911 92-93 21.81924402392696 28.587917194974942 28.086430316269773 21.50640846482833 94-95 21.94685976509204 28.54601223836041 28.0317520461909 21.475375950356646 96-97 21.844695833046245 28.40619209058731 28.10729474946832 21.641817326898124 98-99 22.000151889100366 28.53280998718695 28.009280225366584 21.457757898346095 100 22.02157168375389 28.40438152194143 27.954737409301124 21.61930938500356 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 621.0 1 347.5 2 53.0 3 28.0 4 26.5 5 33.5 6 40.5 7 44.5 8 59.0 9 93.5 10 119.5 11 141.5 12 175.5 13 230.5 14 314.5 15 446.0 16 587.5 17 760.5 18 1068.0 19 1458.5 20 1918.0 21 2588.5 22 3625.5 23 5079.0 24 7137.0 25 10200.0 26 14817.0 27 21721.5 28 31187.5 29 43234.0 30 59745.0 31 80834.0 32 106039.0 33 137250.0 34 174188.0 35 221489.0 36 277837.0 37 339832.0 38 408195.5 39 485054.0 40 565666.0 41 638158.5 42 696863.5 43 739928.5 44 761312.0 45 756807.0 46 728659.5 47 678307.5 48 607087.0 49 520709.0 50 430760.0 51 351936.0 52 284596.0 53 222953.5 54 168723.5 55 121959.5 56 88196.0 57 66157.5 58 48974.5 59 35809.5 60 26795.5 61 20254.0 62 15929.0 63 12843.0 64 10077.0 65 7815.5 66 5880.0 67 4601.0 68 3964.5 69 3247.5 70 2332.0 71 1606.5 72 1315.5 73 1230.0 74 994.0 75 799.0 76 683.5 77 512.0 78 412.0 79 242.0 80 118.0 81 76.0 82 64.5 83 54.5 84 38.5 85 22.5 86 16.5 87 12.5 88 12.5 89 11.5 90 9.0 91 6.5 92 3.5 93 3.5 94 3.5 95 3.0 96 2.0 97 0.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02210609498812023 2 0.0 3 0.0 4 0.0 5 0.007486091807659997 6 0.005129191974367766 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.391478262926895E-4 24-25 7.901484115443301E-4 26-27 0.0 28-29 0.02012846639236928 30-31 0.012172800671562938 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1073869E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.120539856784156 #Duplication Level Percentage of deduplicated Percentage of total 1 67.39869997409855 36.47654028243637 2 18.94212609385006 20.50316180468887 3 6.262907341719556 10.168557792206382 4 2.6615156103136988 5.761706466697429 5 1.3724539643110738 3.7138974738549444 6 0.8262722367910839 2.683097971428363 7 0.5379081346844186 2.037831504973355 8 0.38735088719050725 1.6770911303003642 9 0.2843291995603926 1.3849244799530187 >10 1.2657789617186606 11.672243755225713 >50 0.0479351202620896 1.7557159592351281 >100 0.012638850854045864 1.0034759280176018 >500 5.0228440324428504E-5 0.017368980322713036 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 3.339620546258986E-5 1.1443864706595905 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC 95220 0.8598620771114414 TruSeq Adapter, Index 12 (100% over 50bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG 31412 0.2836587646106343 TruSeq Adapter, Index 12 (100% over 49bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.30473540909685676 9.03026756050663E-6 0.0 9.03026756050663E-6 0.0 2 0.3048076512373408 1.806053512101326E-5 0.0 2.709080268151989E-5 0.0 3 0.3048889236453854 2.709080268151989E-5 0.0 2.709080268151989E-5 0.0 4 0.305078559264156 3.612107024202652E-5 0.0 2.709080268151989E-5 0.0 5 0.305132740869519 3.612107024202652E-5 0.0 3.612107024202652E-5 0.0 6 0.30523207381268463 3.612107024202652E-5 0.0 3.612107024202652E-5 0.0 7 0.3053314067558502 5.418160536303978E-5 0.0 3.612107024202652E-5 0.0 8 0.3054488002341368 5.418160536303978E-5 0.0 3.612107024202652E-5 0.0 9 0.30552104237462085 5.418160536303978E-5 0.0 3.612107024202652E-5 0.0 10-11 0.30576485959875455 5.418160536303978E-5 0.0 3.612107024202652E-5 0.0 12-13 0.3061215551673945 5.418160536303978E-5 0.0 3.612107024202652E-5 0.0 14-15 0.3064827658698148 5.8696739143293096E-5 0.0 3.612107024202652E-5 0.0 16-17 0.3069568549167414 6.321187292354641E-5 0.0 4.063620402227983E-5 0.0 18-19 0.30760703418109786 6.321187292354641E-5 0.0 7.224214048405304E-5 0.0 20-21 0.3082346377765531 6.321187292354641E-5 0.0 9.481780938531961E-5 4.515133780253315E-6 22-23 0.30886675650578854 6.321187292354641E-5 0.0 1.3093887962734615E-4 9.03026756050663E-6 24-25 0.30947178443234247 6.321187292354641E-5 0.0 1.6254481608911933E-4 9.03026756050663E-6 26-27 0.3102077512385238 6.321187292354641E-5 0.0 1.8963561877063923E-4 9.03026756050663E-6 28-29 0.31102047531896937 7.224214048405304E-5 0.0 1.8963561877063923E-4 9.03026756050663E-6 30-31 0.31213119822891167 7.675727426430636E-5 0.0 1.8963561877063923E-4 9.03026756050663E-6 32-33 0.31332770868067883 8.127240804455968E-5 0.0 2.0318102011139918E-4 9.03026756050663E-6 34-35 0.31437521971769755 9.481780938531961E-5 0.0 2.1672642145215913E-4 9.03026756050663E-6 36-37 0.31567557824641057 9.933294316557294E-5 0.0 2.1672642145215913E-4 9.03026756050663E-6 38-39 0.316948845972442 1.0836321072607956E-4 0.0 2.1672642145215913E-4 9.03026756050663E-6 40-41 0.31857880926711346 1.1287834450633287E-4 0.0 2.1672642145215913E-4 9.03026756050663E-6 42-43 0.3202945601036097 1.2190861206683951E-4 0.0 2.1672642145215913E-4 9.03026756050663E-6 44-45 0.32195161420096263 1.3996914718785277E-4 0.0 2.2575668901266577E-4 9.03026756050663E-6 46-47 0.32378927364952576 1.5351454852861272E-4 0.0 2.2575668901266577E-4 9.03026756050663E-6 48-49 0.32594750759648683 1.8060535121013261E-4 0.0 2.5736262547443897E-4 9.03026756050663E-6 50-51 0.32831795283111986 1.8963561877063923E-4 0.0 2.618777592546923E-4 9.03026756050663E-6 52-53 0.3314243648719341 2.0318102011139918E-4 0.0 2.6639289303494564E-4 9.03026756050663E-6 54-55 0.33431856562507645 2.3478695657317238E-4 0.0 2.8896856193621215E-4 9.03026756050663E-6 56-57 0.33760558301710086 2.3478695657317238E-4 0.0 2.8896856193621215E-4 9.03026756050663E-6 58-59 0.3415247191383608 2.663928930349456E-4 0.0 2.8896856193621215E-4 9.03026756050663E-6 60-61 0.34604436805239436 2.979988294967188E-4 0.0 2.8896856193621215E-4 9.03026756050663E-6 62-63 0.35177858795331607 3.0702909705722543E-4 0.0 3.0702909705722543E-4 9.03026756050663E-6 64-65 0.35857837942637755 3.0702909705722543E-4 0.0 3.0702909705722543E-4 9.03026756050663E-6 66-67 0.3654097768359008 3.0702909705722543E-4 0.0 3.1154423083747877E-4 9.03026756050663E-6 68-69 0.37369956245644587 3.0702909705722543E-4 0.0 3.29604765958492E-4 9.03026756050663E-6 70-71 0.38435076304406346 3.0702909705722543E-4 0.0 3.5218043485975856E-4 9.03026756050663E-6 72-73 0.39786907358214185 3.476653010795052E-4 0.0 3.883015051017851E-4 9.03026756050663E-6 74-75 0.4147466436527288 3.7927123754127846E-4 0.0 3.883015051017851E-4 9.03026756050663E-6 76-77 0.43332641915847114 3.9733177266229174E-4 0.0 3.9281663888203846E-4 9.03026756050663E-6 78-79 0.45595626966510083 4.3345284290431825E-4 0.0 4.108771740030517E-4 9.03026756050663E-6 80-81 0.48445127895227946 4.650587793660915E-4 0.0 4.2442257534381164E-4 9.03026756050663E-6 82-83 0.523015036569423 4.831193144871047E-4 0.0 4.2442257534381164E-4 9.03026756050663E-6 84-85 0.574632045945279 5.056949833883713E-4 0.0 4.2442257534381164E-4 9.03026756050663E-6 86-87 0.6375549503068891 5.147252509488779E-4 0.0 4.831193144871047E-4 9.03026756050663E-6 88 0.6874471785786883 5.327857860698912E-4 0.0 5.056949833883713E-4 9.03026756050663E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 22980 0.0 45.058964 3 CGGAAGA 23285 0.0 44.87247 4 ATCGGAA 23920 0.0 43.327553 2 GAGCACA 24425 0.0 43.140427 9 AGAGCAC 24500 0.0 42.89327 8 GATCGGA 24915 0.0 41.55077 1 TATGCCG 17010 0.0 39.53837 46-47 TGCCGTC 17555 0.0 38.217186 48-49 CGTATGC 17605 0.0 38.202084 44-45 CGTCTGA 18090 0.0 37.398872 16-17 CACACGT 18070 0.0 37.375072 12-13 CTCGTAT 18245 0.0 36.83626 42-43 ATCTCGT 18235 0.0 36.79203 40-41 CACGTCT 18445 0.0 36.691654 14-15 CCGTCTT 18325 0.0 36.572865 50-51 GAAGAGC 30595 0.0 34.56457 6 AGTCACC 19890 0.0 33.763885 28-29 TGTAATC 20835 0.0 31.87367 36-37 TAATCTC 21605 0.0 30.911724 38-39 CCAGTCA 22365 0.0 30.185093 26-27 >>END_MODULE skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR3207965 SRR3207965_1.fastq Input file: SRR3207965_1.fastq trimmed: SRR3207965-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Tue Apr 15 04:21:32 2025 >> started Tue Apr 15 04:21:38 2025 >> done (6.159s) 11073869 reads processed; of these: 350 ( 0.00%) short reads filtered out after trimming by size control 136975 ( 1.24%) empty reads filtered out after trimming by size control 10936544 (98.76%) reads available; of these: 7788 ( 0.07%) trimmed reads available after processing 10928756 (99.93%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 42 0.00% 19 33 0.00% 20 41 0.00% 21 32 0.00% 22 38 0.00% 23 34 0.00% 24 28 0.00% 25 49 0.00% 26 38 0.00% 27 42 0.00% 28 59 0.00% 29 58 0.00% 30 73 0.00% 31 65 0.00% 32 60 0.00% 33 54 0.00% 34 71 0.00% 35 77 0.00% 36 68 0.00% 37 68 0.00% 38 85 0.00% 39 99 0.00% 40 99 0.00% 41 97 0.00% 42 98 0.00% 43 96 0.00% 44 95 0.00% 45 99 0.00% 46 120 0.00% 47 120 0.00% 48 121 0.00% 49 119 0.00% 50 174 0.00% 51 186 0.00% 52 161 0.00% 53 157 0.00% 54 171 0.00% 55 189 0.00% 56 185 0.00% 57 232 0.00% 58 229 0.00% 59 246 0.00% 60 337 0.00% 61 319 0.00% 62 359 0.00% 63 414 0.00% 64 368 0.00% 65 378 0.00% 66 418 0.00% 67 439 0.00% 68 548 0.01% 69 0 0.00% 70 0 0.00% 71 0 0.00% 72 0 0.00% 73 0 0.00% 74 0 0.00% 75 0 0.00% 76 0 0.00% 77 0 0.00% 78 0 0.00% 79 0 0.00% 80 0 0.00% 81 0 0.00% 82 0 0.00% 83 0 0.00% 84 0 0.00% 85 0 0.00% 86 0 0.00% 87 0 0.00% 88 0 0.00% 89 0 0.00% 90 0 0.00% 91 0 0.00% 92 0 0.00% 93 0 0.00% 94 0 0.00% 95 0 0.00% 96 0 0.00% 97 0 0.00% 98 0 0.00% 99 0 0.00% 100 10928756 99.93% 10936544 reads passed initial QC criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=43.46 fanout-score-rank=7 prefix-density=0.40 prefix-fanout=32.9 sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=268.54 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=14.5 sequence=CAGCAGCAAGACAAACCGAATTATTCATAAGTACCAATAAAATAAGCATTGCGCAAAAGGGATAGGATAAATCACTCTTAAGCTTGAGGCTTCTCCCATTTGAGGGGCTTGACAACTTCCCAGGTGAAGTCTGGGTCATCCCTTCCAAAATGTCCGTATGCAGCTGTCTTCAAGAACCTATTACCCCCCCTCTTGAG Started job on | Apr 15 04:21:54 Started mapping on | Apr 15 04:21:54 Finished on | Apr 15 04:22:09 Mapping speed, Million of reads per hour | 2624.77 Number of input reads | 10936544 Average input read length | 99 UNIQUE READS: Uniquely mapped reads number | 10396671 Uniquely mapped reads % | 95.06% Average mapped length | 99.61 Number of splices: Total | 3070841 Number of splices: Annotated (sjdb) | 3014329 Number of splices: GT/AG | 3024950 Number of splices: GC/AG | 37578 Number of splices: AT/AC | 3061 Number of splices: Non-canonical | 5252 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.02% Deletion average length | 1.98 Insertion rate per base | 0.02% Insertion average length | 1.47 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 213523 % of reads mapped to multiple loci | 1.95% Number of reads mapped to too many loci | 61796 % of reads mapped to too many loci | 0.57% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.41% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 326350 326350 326350 N_multimapping 213523 213523 213523 N_noFeature 472173 5366098 5431772 N_ambiguous 106945 18141 17997 UnstrandedReadsAssigned:9817553 PositiveStrandReadsAssigned:5012432 NegativeStrandReadsAssigned:4946902 Dataset is classified unstranded MeadianReadLen=100 20thPercentileLength=100 echo kmer=95 SRR3207965 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR3207965-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 10,936,544 reads, 10,084,557 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,232 rounds 52401 SRR3207965.ke.tsv 34699 SRR3207965.se.tsv 87100 total ==> SRR3207965.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 286 22.2844 Potri.005G024800.1.v4.1 1035 936 46 7.34839 Potri.004G059700.1.v4.1 961 862 5 0.867307 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 146.284 7.69089 Potri.016G087400.1.v4.1 270 171 436 381.242 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 31 2.76896 Potri.012G127500.1.v4.1 977 878 870 148.161 ==> SRR3207965.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 1242 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 213 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 12 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 4 SRR3207965 completed mapping pipeline successfully