Starting /dee2/code/volunteer_pipeline.sh SRR3208000 current disk space = 2796418781184 free memory = 1575040984 SRR3208000_1.fastq is conventional basespace SRR3208000_1.fastq read1 length is 125 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3208000_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20723749 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0 30.0 30.0 30.0 30.0 30.0 2 30.0 30.0 30.0 30.0 30.0 30.0 3 30.0 30.0 30.0 30.0 30.0 30.0 4 30.0 30.0 30.0 30.0 30.0 30.0 5 30.0 30.0 30.0 30.0 30.0 30.0 6 30.0 30.0 30.0 30.0 30.0 30.0 7 30.0 30.0 30.0 30.0 30.0 30.0 8 30.0 30.0 30.0 30.0 30.0 30.0 9 30.0 30.0 30.0 30.0 30.0 30.0 10-11 30.0 30.0 30.0 30.0 30.0 30.0 12-13 30.0 30.0 30.0 30.0 30.0 30.0 14-15 30.0 30.0 30.0 30.0 30.0 30.0 16-17 30.0 30.0 30.0 30.0 30.0 30.0 18-19 30.0 30.0 30.0 30.0 30.0 30.0 20-21 30.0 30.0 30.0 30.0 30.0 30.0 22-23 30.0 30.0 30.0 30.0 30.0 30.0 24-25 30.0 30.0 30.0 30.0 30.0 30.0 26-27 30.0 30.0 30.0 30.0 30.0 30.0 28-29 30.0 30.0 30.0 30.0 30.0 30.0 30-31 30.0 30.0 30.0 30.0 30.0 30.0 32-33 30.0 30.0 30.0 30.0 30.0 30.0 34-35 30.0 30.0 30.0 30.0 30.0 30.0 36-37 30.0 30.0 30.0 30.0 30.0 30.0 38-39 30.0 30.0 30.0 30.0 30.0 30.0 40-41 30.0 30.0 30.0 30.0 30.0 30.0 42-43 30.0 30.0 30.0 30.0 30.0 30.0 44-45 30.0 30.0 30.0 30.0 30.0 30.0 46-47 30.0 30.0 30.0 30.0 30.0 30.0 48-49 30.0 30.0 30.0 30.0 30.0 30.0 50-51 30.0 30.0 30.0 30.0 30.0 30.0 52-53 30.0 30.0 30.0 30.0 30.0 30.0 54-55 30.0 30.0 30.0 30.0 30.0 30.0 56-57 30.0 30.0 30.0 30.0 30.0 30.0 58-59 30.0 30.0 30.0 30.0 30.0 30.0 60-61 30.0 30.0 30.0 30.0 30.0 30.0 62-63 30.0 30.0 30.0 30.0 30.0 30.0 64-65 30.0 30.0 30.0 30.0 30.0 30.0 66-67 30.0 30.0 30.0 30.0 30.0 30.0 68-69 30.0 30.0 30.0 30.0 30.0 30.0 70-71 30.0 30.0 30.0 30.0 30.0 30.0 72-73 30.0 30.0 30.0 30.0 30.0 30.0 74-75 30.0 30.0 30.0 30.0 30.0 30.0 76-77 30.0 30.0 30.0 30.0 30.0 30.0 78-79 30.0 30.0 30.0 30.0 30.0 30.0 80-81 30.0 30.0 30.0 30.0 30.0 30.0 82-83 30.0 30.0 30.0 30.0 30.0 30.0 84-85 30.0 30.0 30.0 30.0 30.0 30.0 86-87 30.0 30.0 30.0 30.0 30.0 30.0 88-89 30.0 30.0 30.0 30.0 30.0 30.0 90-91 30.0 30.0 30.0 30.0 30.0 30.0 92-93 30.0 30.0 30.0 30.0 30.0 30.0 94-95 30.0 30.0 30.0 30.0 30.0 30.0 96-97 30.0 30.0 30.0 30.0 30.0 30.0 98-99 30.0 30.0 30.0 30.0 30.0 30.0 100-101 30.0 30.0 30.0 30.0 30.0 30.0 102-103 30.0 30.0 30.0 30.0 30.0 30.0 104-105 30.0 30.0 30.0 30.0 30.0 30.0 106-107 30.0 30.0 30.0 30.0 30.0 30.0 108-109 30.0 30.0 30.0 30.0 30.0 30.0 110-111 30.0 30.0 30.0 30.0 30.0 30.0 112-113 30.0 30.0 30.0 30.0 30.0 30.0 114-115 30.0 30.0 30.0 30.0 30.0 30.0 116-117 30.0 30.0 30.0 30.0 30.0 30.0 118-119 30.0 30.0 30.0 30.0 30.0 30.0 120-121 30.0 30.0 30.0 30.0 30.0 30.0 122-123 30.0 30.0 30.0 30.0 30.0 30.0 124-125 30.0 30.0 30.0 30.0 30.0 30.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-11 0.0 1101 12-13 0.0 1101 14-15 0.0 1101 16-17 0.0 1101 18-19 0.0 1101 20-21 0.0 1101 22-23 0.0 1101 24-25 0.0 1101 26-27 0.0 1101 28-29 0.0 1101 30-31 0.0 1101 32-33 0.0 1101 34-35 0.0 1101 36-37 0.0 1101 38-39 0.0 1101 40-41 0.0 1101 42-43 0.0 1101 44-45 0.0 1101 46-47 0.0 1101 48-49 0.0 1101 50-51 0.0 1101 52-53 0.0 1101 54-55 0.0 1101 56-57 0.0 1101 58-59 0.0 1101 60-61 0.0 1101 62-63 0.0 1101 64-65 0.0 1101 66-67 0.0 1101 68-69 0.0 1101 70-71 0.0 1101 72-73 0.0 1101 74-75 0.0 1101 76-77 0.0 1101 78-79 0.0 1101 80-81 0.0 1101 82-83 0.0 1101 84-85 0.0 1101 86-87 0.0 1101 88-89 0.0 1101 90-91 0.0 1101 92-93 0.0 1101 94-95 0.0 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.0 1102 2 0.0 1102 3 0.0 1102 4 0.0 1102 5 0.0 1102 6 0.0 1102 7 0.0 1102 8 0.0 1102 9 0.0 1102 10-11 0.0 1102 12-13 0.0 1102 14-15 0.0 1102 16-17 0.0 1102 18-19 0.0 1102 20-21 0.0 1102 22-23 0.0 1102 24-25 0.0 1102 26-27 0.0 1102 28-29 0.0 1102 30-31 0.0 1102 32-33 0.0 1102 34-35 0.0 1102 36-37 0.0 1102 38-39 0.0 1102 40-41 0.0 1102 42-43 0.0 1102 44-45 0.0 1102 46-47 0.0 1102 48-49 0.0 1102 50-51 0.0 1102 52-53 0.0 1102 54-55 0.0 1102 56-57 0.0 1102 58-59 0.0 1102 60-61 0.0 1102 62-63 0.0 1102 64-65 0.0 1102 66-67 0.0 1102 68-69 0.0 1102 70-71 0.0 1102 72-73 0.0 1102 74-75 0.0 1102 76-77 0.0 1102 78-79 0.0 1102 80-81 0.0 1102 82-83 0.0 1102 84-85 0.0 1102 86-87 0.0 1102 88-89 0.0 1102 90-91 0.0 1102 92-93 0.0 1102 94-95 0.0 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.0 1103 2 0.0 1103 3 0.0 1103 4 0.0 1103 5 0.0 1103 6 0.0 1103 7 0.0 1103 8 0.0 1103 9 0.0 1103 10-11 0.0 1103 12-13 0.0 1103 14-15 0.0 1103 16-17 0.0 1103 18-19 0.0 1103 20-21 0.0 1103 22-23 0.0 1103 24-25 0.0 1103 26-27 0.0 1103 28-29 0.0 1103 30-31 0.0 1103 32-33 0.0 1103 34-35 0.0 1103 36-37 0.0 1103 38-39 0.0 1103 40-41 0.0 1103 42-43 0.0 1103 44-45 0.0 1103 46-47 0.0 1103 48-49 0.0 1103 50-51 0.0 1103 52-53 0.0 1103 54-55 0.0 1103 56-57 0.0 1103 58-59 0.0 1103 60-61 0.0 1103 62-63 0.0 1103 64-65 0.0 1103 66-67 0.0 1103 68-69 0.0 1103 70-71 0.0 1103 72-73 0.0 1103 74-75 0.0 1103 76-77 0.0 1103 78-79 0.0 1103 80-81 0.0 1103 82-83 0.0 1103 84-85 0.0 1103 86-87 0.0 1103 88-89 0.0 1103 90-91 0.0 1103 92-93 0.0 1103 94-95 0.0 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 0.0 1104 2 0.0 1104 3 0.0 1104 4 0.0 1104 5 0.0 1104 6 0.0 1104 7 0.0 1104 8 0.0 1104 9 0.0 1104 10-11 0.0 1104 12-13 0.0 1104 14-15 0.0 1104 16-17 0.0 1104 18-19 0.0 1104 20-21 0.0 1104 22-23 0.0 1104 24-25 0.0 1104 26-27 0.0 1104 28-29 0.0 1104 30-31 0.0 1104 32-33 0.0 1104 34-35 0.0 1104 36-37 0.0 1104 38-39 0.0 1104 40-41 0.0 1104 42-43 0.0 1104 44-45 0.0 1104 46-47 0.0 1104 48-49 0.0 1104 50-51 0.0 1104 52-53 0.0 1104 54-55 0.0 1104 56-57 0.0 1104 58-59 0.0 1104 60-61 0.0 1104 62-63 0.0 1104 64-65 0.0 1104 66-67 0.0 1104 68-69 0.0 1104 70-71 0.0 1104 72-73 0.0 1104 74-75 0.0 1104 76-77 0.0 1104 78-79 0.0 1104 80-81 0.0 1104 82-83 0.0 1104 84-85 0.0 1104 86-87 0.0 1104 88-89 0.0 1104 90-91 0.0 1104 92-93 0.0 1104 94-95 0.0 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 0.0 1105 2 0.0 1105 3 0.0 1105 4 0.0 1105 5 0.0 1105 6 0.0 1105 7 0.0 1105 8 0.0 1105 9 0.0 1105 10-11 0.0 1105 12-13 0.0 1105 14-15 0.0 1105 16-17 0.0 1105 18-19 0.0 1105 20-21 0.0 1105 22-23 0.0 1105 24-25 0.0 1105 26-27 0.0 1105 28-29 0.0 1105 30-31 0.0 1105 32-33 0.0 1105 34-35 0.0 1105 36-37 0.0 1105 38-39 0.0 1105 40-41 0.0 1105 42-43 0.0 1105 44-45 0.0 1105 46-47 0.0 1105 48-49 0.0 1105 50-51 0.0 1105 52-53 0.0 1105 54-55 0.0 1105 56-57 0.0 1105 58-59 0.0 1105 60-61 0.0 1105 62-63 0.0 1105 64-65 0.0 1105 66-67 0.0 1105 68-69 0.0 1105 70-71 0.0 1105 72-73 0.0 1105 74-75 0.0 1105 76-77 0.0 1105 78-79 0.0 1105 80-81 0.0 1105 82-83 0.0 1105 84-85 0.0 1105 86-87 0.0 1105 88-89 0.0 1105 90-91 0.0 1105 92-93 0.0 1105 94-95 0.0 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 0.0 1106 2 0.0 1106 3 0.0 1106 4 0.0 1106 5 0.0 1106 6 0.0 1106 7 0.0 1106 8 0.0 1106 9 0.0 1106 10-11 0.0 1106 12-13 0.0 1106 14-15 0.0 1106 16-17 0.0 1106 18-19 0.0 1106 20-21 0.0 1106 22-23 0.0 1106 24-25 0.0 1106 26-27 0.0 1106 28-29 0.0 1106 30-31 0.0 1106 32-33 0.0 1106 34-35 0.0 1106 36-37 0.0 1106 38-39 0.0 1106 40-41 0.0 1106 42-43 0.0 1106 44-45 0.0 1106 46-47 0.0 1106 48-49 0.0 1106 50-51 0.0 1106 52-53 0.0 1106 54-55 0.0 1106 56-57 0.0 1106 58-59 0.0 1106 60-61 0.0 1106 62-63 0.0 1106 64-65 0.0 1106 66-67 0.0 1106 68-69 0.0 1106 70-71 0.0 1106 72-73 0.0 1106 74-75 0.0 1106 76-77 0.0 1106 78-79 0.0 1106 80-81 0.0 1106 82-83 0.0 1106 84-85 0.0 1106 86-87 0.0 1106 88-89 0.0 1106 90-91 0.0 1106 92-93 0.0 1106 94-95 0.0 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 0.0 1107 2 0.0 1107 3 0.0 1107 4 0.0 1107 5 0.0 1107 6 0.0 1107 7 0.0 1107 8 0.0 1107 9 0.0 1107 10-11 0.0 1107 12-13 0.0 1107 14-15 0.0 1107 16-17 0.0 1107 18-19 0.0 1107 20-21 0.0 1107 22-23 0.0 1107 24-25 0.0 1107 26-27 0.0 1107 28-29 0.0 1107 30-31 0.0 1107 32-33 0.0 1107 34-35 0.0 1107 36-37 0.0 1107 38-39 0.0 1107 40-41 0.0 1107 42-43 0.0 1107 44-45 0.0 1107 46-47 0.0 1107 48-49 0.0 1107 50-51 0.0 1107 52-53 0.0 1107 54-55 0.0 1107 56-57 0.0 1107 58-59 0.0 1107 60-61 0.0 1107 62-63 0.0 1107 64-65 0.0 1107 66-67 0.0 1107 68-69 0.0 1107 70-71 0.0 1107 72-73 0.0 1107 74-75 0.0 1107 76-77 0.0 1107 78-79 0.0 1107 80-81 0.0 1107 82-83 0.0 1107 84-85 0.0 1107 86-87 0.0 1107 88-89 0.0 1107 90-91 0.0 1107 92-93 0.0 1107 94-95 0.0 1107 96-97 0.0 1107 98-99 0.0 1107 100-101 0.0 1107 102-103 0.0 1107 104-105 0.0 1107 106-107 0.0 1107 108-109 0.0 1107 110-111 0.0 1107 112-113 0.0 1107 114-115 0.0 1107 116-117 0.0 1107 118-119 0.0 1107 120-121 0.0 1107 122-123 0.0 1107 124-125 0.0 1108 1 0.0 1108 2 0.0 1108 3 0.0 1108 4 0.0 1108 5 0.0 1108 6 0.0 1108 7 0.0 1108 8 0.0 1108 9 0.0 1108 10-11 0.0 1108 12-13 0.0 1108 14-15 0.0 1108 16-17 0.0 1108 18-19 0.0 1108 20-21 0.0 1108 22-23 0.0 1108 24-25 0.0 1108 26-27 0.0 1108 28-29 0.0 1108 30-31 0.0 1108 32-33 0.0 1108 34-35 0.0 1108 36-37 0.0 1108 38-39 0.0 1108 40-41 0.0 1108 42-43 0.0 1108 44-45 0.0 1108 46-47 0.0 1108 48-49 0.0 1108 50-51 0.0 1108 52-53 0.0 1108 54-55 0.0 1108 56-57 0.0 1108 58-59 0.0 1108 60-61 0.0 1108 62-63 0.0 1108 64-65 0.0 1108 66-67 0.0 1108 68-69 0.0 1108 70-71 0.0 1108 72-73 0.0 1108 74-75 0.0 1108 76-77 0.0 1108 78-79 0.0 1108 80-81 0.0 1108 82-83 0.0 1108 84-85 0.0 1108 86-87 0.0 1108 88-89 0.0 1108 90-91 0.0 1108 92-93 0.0 1108 94-95 0.0 1108 96-97 0.0 1108 98-99 0.0 1108 100-101 0.0 1108 102-103 0.0 1108 104-105 0.0 1108 106-107 0.0 1108 108-109 0.0 1108 110-111 0.0 1108 112-113 0.0 1108 114-115 0.0 1108 116-117 0.0 1108 118-119 0.0 1108 120-121 0.0 1108 122-123 0.0 1108 124-125 0.0 1109 1 0.0 1109 2 0.0 1109 3 0.0 1109 4 0.0 1109 5 0.0 1109 6 0.0 1109 7 0.0 1109 8 0.0 1109 9 0.0 1109 10-11 0.0 1109 12-13 0.0 1109 14-15 0.0 1109 16-17 0.0 1109 18-19 0.0 1109 20-21 0.0 1109 22-23 0.0 1109 24-25 0.0 1109 26-27 0.0 1109 28-29 0.0 1109 30-31 0.0 1109 32-33 0.0 1109 34-35 0.0 1109 36-37 0.0 1109 38-39 0.0 1109 40-41 0.0 1109 42-43 0.0 1109 44-45 0.0 1109 46-47 0.0 1109 48-49 0.0 1109 50-51 0.0 1109 52-53 0.0 1109 54-55 0.0 1109 56-57 0.0 1109 58-59 0.0 1109 60-61 0.0 1109 62-63 0.0 1109 64-65 0.0 1109 66-67 0.0 1109 68-69 0.0 1109 70-71 0.0 1109 72-73 0.0 1109 74-75 0.0 1109 76-77 0.0 1109 78-79 0.0 1109 80-81 0.0 1109 82-83 0.0 1109 84-85 0.0 1109 86-87 0.0 1109 88-89 0.0 1109 90-91 0.0 1109 92-93 0.0 1109 94-95 0.0 1109 96-97 0.0 1109 98-99 0.0 1109 100-101 0.0 1109 102-103 0.0 1109 104-105 0.0 1109 106-107 0.0 1109 108-109 0.0 1109 110-111 0.0 1109 112-113 0.0 1109 114-115 0.0 1109 116-117 0.0 1109 118-119 0.0 1109 120-121 0.0 1109 122-123 0.0 1109 124-125 0.0 1110 1 0.0 1110 2 0.0 1110 3 0.0 1110 4 0.0 1110 5 0.0 1110 6 0.0 1110 7 0.0 1110 8 0.0 1110 9 0.0 1110 10-11 0.0 1110 12-13 0.0 1110 14-15 0.0 1110 16-17 0.0 1110 18-19 0.0 1110 20-21 0.0 1110 22-23 0.0 1110 24-25 0.0 1110 26-27 0.0 1110 28-29 0.0 1110 30-31 0.0 1110 32-33 0.0 1110 34-35 0.0 1110 36-37 0.0 1110 38-39 0.0 1110 40-41 0.0 1110 42-43 0.0 1110 44-45 0.0 1110 46-47 0.0 1110 48-49 0.0 1110 50-51 0.0 1110 52-53 0.0 1110 54-55 0.0 1110 56-57 0.0 1110 58-59 0.0 1110 60-61 0.0 1110 62-63 0.0 1110 64-65 0.0 1110 66-67 0.0 1110 68-69 0.0 1110 70-71 0.0 1110 72-73 0.0 1110 74-75 0.0 1110 76-77 0.0 1110 78-79 0.0 1110 80-81 0.0 1110 82-83 0.0 1110 84-85 0.0 1110 86-87 0.0 1110 88-89 0.0 1110 90-91 0.0 1110 92-93 0.0 1110 94-95 0.0 1110 96-97 0.0 1110 98-99 0.0 1110 100-101 0.0 1110 102-103 0.0 1110 104-105 0.0 1110 106-107 0.0 1110 108-109 0.0 1110 110-111 0.0 1110 112-113 0.0 1110 114-115 0.0 1110 116-117 0.0 1110 118-119 0.0 1110 120-121 0.0 1110 122-123 0.0 1110 124-125 0.0 1111 1 0.0 1111 2 0.0 1111 3 0.0 1111 4 0.0 1111 5 0.0 1111 6 0.0 1111 7 0.0 1111 8 0.0 1111 9 0.0 1111 10-11 0.0 1111 12-13 0.0 1111 14-15 0.0 1111 16-17 0.0 1111 18-19 0.0 1111 20-21 0.0 1111 22-23 0.0 1111 24-25 0.0 1111 26-27 0.0 1111 28-29 0.0 1111 30-31 0.0 1111 32-33 0.0 1111 34-35 0.0 1111 36-37 0.0 1111 38-39 0.0 1111 40-41 0.0 1111 42-43 0.0 1111 44-45 0.0 1111 46-47 0.0 1111 48-49 0.0 1111 50-51 0.0 1111 52-53 0.0 1111 54-55 0.0 1111 56-57 0.0 1111 58-59 0.0 1111 60-61 0.0 1111 62-63 0.0 1111 64-65 0.0 1111 66-67 0.0 1111 68-69 0.0 1111 70-71 0.0 1111 72-73 0.0 1111 74-75 0.0 1111 76-77 0.0 1111 78-79 0.0 1111 80-81 0.0 1111 82-83 0.0 1111 84-85 0.0 1111 86-87 0.0 1111 88-89 0.0 1111 90-91 0.0 1111 92-93 0.0 1111 94-95 0.0 1111 96-97 0.0 1111 98-99 0.0 1111 100-101 0.0 1111 102-103 0.0 1111 104-105 0.0 1111 106-107 0.0 1111 108-109 0.0 1111 110-111 0.0 1111 112-113 0.0 1111 114-115 0.0 1111 116-117 0.0 1111 118-119 0.0 1111 120-121 0.0 1111 122-123 0.0 1111 124-125 0.0 1112 1 0.0 1112 2 0.0 1112 3 0.0 1112 4 0.0 1112 5 0.0 1112 6 0.0 1112 7 0.0 1112 8 0.0 1112 9 0.0 1112 10-11 0.0 1112 12-13 0.0 1112 14-15 0.0 1112 16-17 0.0 1112 18-19 0.0 1112 20-21 0.0 1112 22-23 0.0 1112 24-25 0.0 1112 26-27 0.0 1112 28-29 0.0 1112 30-31 0.0 1112 32-33 0.0 1112 34-35 0.0 1112 36-37 0.0 1112 38-39 0.0 1112 40-41 0.0 1112 42-43 0.0 1112 44-45 0.0 1112 46-47 0.0 1112 48-49 0.0 1112 50-51 0.0 1112 52-53 0.0 1112 54-55 0.0 1112 56-57 0.0 1112 58-59 0.0 1112 60-61 0.0 1112 62-63 0.0 1112 64-65 0.0 1112 66-67 0.0 1112 68-69 0.0 1112 70-71 0.0 1112 72-73 0.0 1112 74-75 0.0 1112 76-77 0.0 1112 78-79 0.0 1112 80-81 0.0 1112 82-83 0.0 1112 84-85 0.0 1112 86-87 0.0 1112 88-89 0.0 1112 90-91 0.0 1112 92-93 0.0 1112 94-95 0.0 1112 96-97 0.0 1112 98-99 0.0 1112 100-101 0.0 1112 102-103 0.0 1112 104-105 0.0 1112 106-107 0.0 1112 108-109 0.0 1112 110-111 0.0 1112 112-113 0.0 1112 114-115 0.0 1112 116-117 0.0 1112 118-119 0.0 1112 120-121 0.0 1112 122-123 0.0 1112 124-125 0.0 1113 1 0.0 1113 2 0.0 1113 3 0.0 1113 4 0.0 1113 5 0.0 1113 6 0.0 1113 7 0.0 1113 8 0.0 1113 9 0.0 1113 10-11 0.0 1113 12-13 0.0 1113 14-15 0.0 1113 16-17 0.0 1113 18-19 0.0 1113 20-21 0.0 1113 22-23 0.0 1113 24-25 0.0 1113 26-27 0.0 1113 28-29 0.0 1113 30-31 0.0 1113 32-33 0.0 1113 34-35 0.0 1113 36-37 0.0 1113 38-39 0.0 1113 40-41 0.0 1113 42-43 0.0 1113 44-45 0.0 1113 46-47 0.0 1113 48-49 0.0 1113 50-51 0.0 1113 52-53 0.0 1113 54-55 0.0 1113 56-57 0.0 1113 58-59 0.0 1113 60-61 0.0 1113 62-63 0.0 1113 64-65 0.0 1113 66-67 0.0 1113 68-69 0.0 1113 70-71 0.0 1113 72-73 0.0 1113 74-75 0.0 1113 76-77 0.0 1113 78-79 0.0 1113 80-81 0.0 1113 82-83 0.0 1113 84-85 0.0 1113 86-87 0.0 1113 88-89 0.0 1113 90-91 0.0 1113 92-93 0.0 1113 94-95 0.0 1113 96-97 0.0 1113 98-99 0.0 1113 100-101 0.0 1113 102-103 0.0 1113 104-105 0.0 1113 106-107 0.0 1113 108-109 0.0 1113 110-111 0.0 1113 112-113 0.0 1113 114-115 0.0 1113 116-117 0.0 1113 118-119 0.0 1113 120-121 0.0 1113 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122-123 0.0 2310 124-125 0.0 2311 1 0.0 2311 2 0.0 2311 3 0.0 2311 4 0.0 2311 5 0.0 2311 6 0.0 2311 7 0.0 2311 8 0.0 2311 9 0.0 2311 10-11 0.0 2311 12-13 0.0 2311 14-15 0.0 2311 16-17 0.0 2311 18-19 0.0 2311 20-21 0.0 2311 22-23 0.0 2311 24-25 0.0 2311 26-27 0.0 2311 28-29 0.0 2311 30-31 0.0 2311 32-33 0.0 2311 34-35 0.0 2311 36-37 0.0 2311 38-39 0.0 2311 40-41 0.0 2311 42-43 0.0 2311 44-45 0.0 2311 46-47 0.0 2311 48-49 0.0 2311 50-51 0.0 2311 52-53 0.0 2311 54-55 0.0 2311 56-57 0.0 2311 58-59 0.0 2311 60-61 0.0 2311 62-63 0.0 2311 64-65 0.0 2311 66-67 0.0 2311 68-69 0.0 2311 70-71 0.0 2311 72-73 0.0 2311 74-75 0.0 2311 76-77 0.0 2311 78-79 0.0 2311 80-81 0.0 2311 82-83 0.0 2311 84-85 0.0 2311 86-87 0.0 2311 88-89 0.0 2311 90-91 0.0 2311 92-93 0.0 2311 94-95 0.0 2311 96-97 0.0 2311 98-99 0.0 2311 100-101 0.0 2311 102-103 0.0 2311 104-105 0.0 2311 106-107 0.0 2311 108-109 0.0 2311 110-111 0.0 2311 112-113 0.0 2311 114-115 0.0 2311 116-117 0.0 2311 118-119 0.0 2311 120-121 0.0 2311 122-123 0.0 2311 124-125 0.0 2312 1 0.0 2312 2 0.0 2312 3 0.0 2312 4 0.0 2312 5 0.0 2312 6 0.0 2312 7 0.0 2312 8 0.0 2312 9 0.0 2312 10-11 0.0 2312 12-13 0.0 2312 14-15 0.0 2312 16-17 0.0 2312 18-19 0.0 2312 20-21 0.0 2312 22-23 0.0 2312 24-25 0.0 2312 26-27 0.0 2312 28-29 0.0 2312 30-31 0.0 2312 32-33 0.0 2312 34-35 0.0 2312 36-37 0.0 2312 38-39 0.0 2312 40-41 0.0 2312 42-43 0.0 2312 44-45 0.0 2312 46-47 0.0 2312 48-49 0.0 2312 50-51 0.0 2312 52-53 0.0 2312 54-55 0.0 2312 56-57 0.0 2312 58-59 0.0 2312 60-61 0.0 2312 62-63 0.0 2312 64-65 0.0 2312 66-67 0.0 2312 68-69 0.0 2312 70-71 0.0 2312 72-73 0.0 2312 74-75 0.0 2312 76-77 0.0 2312 78-79 0.0 2312 80-81 0.0 2312 82-83 0.0 2312 84-85 0.0 2312 86-87 0.0 2312 88-89 0.0 2312 90-91 0.0 2312 92-93 0.0 2312 94-95 0.0 2312 96-97 0.0 2312 98-99 0.0 2312 100-101 0.0 2312 102-103 0.0 2312 104-105 0.0 2312 106-107 0.0 2312 108-109 0.0 2312 110-111 0.0 2312 112-113 0.0 2312 114-115 0.0 2312 116-117 0.0 2312 118-119 0.0 2312 120-121 0.0 2312 122-123 0.0 2312 124-125 0.0 2313 1 0.0 2313 2 0.0 2313 3 0.0 2313 4 0.0 2313 5 0.0 2313 6 0.0 2313 7 0.0 2313 8 0.0 2313 9 0.0 2313 10-11 0.0 2313 12-13 0.0 2313 14-15 0.0 2313 16-17 0.0 2313 18-19 0.0 2313 20-21 0.0 2313 22-23 0.0 2313 24-25 0.0 2313 26-27 0.0 2313 28-29 0.0 2313 30-31 0.0 2313 32-33 0.0 2313 34-35 0.0 2313 36-37 0.0 2313 38-39 0.0 2313 40-41 0.0 2313 42-43 0.0 2313 44-45 0.0 2313 46-47 0.0 2313 48-49 0.0 2313 50-51 0.0 2313 52-53 0.0 2313 54-55 0.0 2313 56-57 0.0 2313 58-59 0.0 2313 60-61 0.0 2313 62-63 0.0 2313 64-65 0.0 2313 66-67 0.0 2313 68-69 0.0 2313 70-71 0.0 2313 72-73 0.0 2313 74-75 0.0 2313 76-77 0.0 2313 78-79 0.0 2313 80-81 0.0 2313 82-83 0.0 2313 84-85 0.0 2313 86-87 0.0 2313 88-89 0.0 2313 90-91 0.0 2313 92-93 0.0 2313 94-95 0.0 2313 96-97 0.0 2313 98-99 0.0 2313 100-101 0.0 2313 102-103 0.0 2313 104-105 0.0 2313 106-107 0.0 2313 108-109 0.0 2313 110-111 0.0 2313 112-113 0.0 2313 114-115 0.0 2313 116-117 0.0 2313 118-119 0.0 2313 120-121 0.0 2313 122-123 0.0 2313 124-125 0.0 2314 1 0.0 2314 2 0.0 2314 3 0.0 2314 4 0.0 2314 5 0.0 2314 6 0.0 2314 7 0.0 2314 8 0.0 2314 9 0.0 2314 10-11 0.0 2314 12-13 0.0 2314 14-15 0.0 2314 16-17 0.0 2314 18-19 0.0 2314 20-21 0.0 2314 22-23 0.0 2314 24-25 0.0 2314 26-27 0.0 2314 28-29 0.0 2314 30-31 0.0 2314 32-33 0.0 2314 34-35 0.0 2314 36-37 0.0 2314 38-39 0.0 2314 40-41 0.0 2314 42-43 0.0 2314 44-45 0.0 2314 46-47 0.0 2314 48-49 0.0 2314 50-51 0.0 2314 52-53 0.0 2314 54-55 0.0 2314 56-57 0.0 2314 58-59 0.0 2314 60-61 0.0 2314 62-63 0.0 2314 64-65 0.0 2314 66-67 0.0 2314 68-69 0.0 2314 70-71 0.0 2314 72-73 0.0 2314 74-75 0.0 2314 76-77 0.0 2314 78-79 0.0 2314 80-81 0.0 2314 82-83 0.0 2314 84-85 0.0 2314 86-87 0.0 2314 88-89 0.0 2314 90-91 0.0 2314 92-93 0.0 2314 94-95 0.0 2314 96-97 0.0 2314 98-99 0.0 2314 100-101 0.0 2314 102-103 0.0 2314 104-105 0.0 2314 106-107 0.0 2314 108-109 0.0 2314 110-111 0.0 2314 112-113 0.0 2314 114-115 0.0 2314 116-117 0.0 2314 118-119 0.0 2314 120-121 0.0 2314 122-123 0.0 2314 124-125 0.0 2315 1 0.0 2315 2 0.0 2315 3 0.0 2315 4 0.0 2315 5 0.0 2315 6 0.0 2315 7 0.0 2315 8 0.0 2315 9 0.0 2315 10-11 0.0 2315 12-13 0.0 2315 14-15 0.0 2315 16-17 0.0 2315 18-19 0.0 2315 20-21 0.0 2315 22-23 0.0 2315 24-25 0.0 2315 26-27 0.0 2315 28-29 0.0 2315 30-31 0.0 2315 32-33 0.0 2315 34-35 0.0 2315 36-37 0.0 2315 38-39 0.0 2315 40-41 0.0 2315 42-43 0.0 2315 44-45 0.0 2315 46-47 0.0 2315 48-49 0.0 2315 50-51 0.0 2315 52-53 0.0 2315 54-55 0.0 2315 56-57 0.0 2315 58-59 0.0 2315 60-61 0.0 2315 62-63 0.0 2315 64-65 0.0 2315 66-67 0.0 2315 68-69 0.0 2315 70-71 0.0 2315 72-73 0.0 2315 74-75 0.0 2315 76-77 0.0 2315 78-79 0.0 2315 80-81 0.0 2315 82-83 0.0 2315 84-85 0.0 2315 86-87 0.0 2315 88-89 0.0 2315 90-91 0.0 2315 92-93 0.0 2315 94-95 0.0 2315 96-97 0.0 2315 98-99 0.0 2315 100-101 0.0 2315 102-103 0.0 2315 104-105 0.0 2315 106-107 0.0 2315 108-109 0.0 2315 110-111 0.0 2315 112-113 0.0 2315 114-115 0.0 2315 116-117 0.0 2315 118-119 0.0 2315 120-121 0.0 2315 122-123 0.0 2315 124-125 0.0 2316 1 0.0 2316 2 0.0 2316 3 0.0 2316 4 0.0 2316 5 0.0 2316 6 0.0 2316 7 0.0 2316 8 0.0 2316 9 0.0 2316 10-11 0.0 2316 12-13 0.0 2316 14-15 0.0 2316 16-17 0.0 2316 18-19 0.0 2316 20-21 0.0 2316 22-23 0.0 2316 24-25 0.0 2316 26-27 0.0 2316 28-29 0.0 2316 30-31 0.0 2316 32-33 0.0 2316 34-35 0.0 2316 36-37 0.0 2316 38-39 0.0 2316 40-41 0.0 2316 42-43 0.0 2316 44-45 0.0 2316 46-47 0.0 2316 48-49 0.0 2316 50-51 0.0 2316 52-53 0.0 2316 54-55 0.0 2316 56-57 0.0 2316 58-59 0.0 2316 60-61 0.0 2316 62-63 0.0 2316 64-65 0.0 2316 66-67 0.0 2316 68-69 0.0 2316 70-71 0.0 2316 72-73 0.0 2316 74-75 0.0 2316 76-77 0.0 2316 78-79 0.0 2316 80-81 0.0 2316 82-83 0.0 2316 84-85 0.0 2316 86-87 0.0 2316 88-89 0.0 2316 90-91 0.0 2316 92-93 0.0 2316 94-95 0.0 2316 96-97 0.0 2316 98-99 0.0 2316 100-101 0.0 2316 102-103 0.0 2316 104-105 0.0 2316 106-107 0.0 2316 108-109 0.0 2316 110-111 0.0 2316 112-113 0.0 2316 114-115 0.0 2316 116-117 0.0 2316 118-119 0.0 2316 120-121 0.0 2316 122-123 0.0 2316 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 2.0723749E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.76583652273513 15.49757226368828 10.645145594184516 52.091445619392076 2 17.44809288595735 20.74302298305785 42.79225956790809 19.01662456307671 3 19.44942973397333 25.061932568281925 29.35065465230253 26.137983045442216 4 24.219280943629997 30.452728651560367 21.965804781343266 23.36218562346637 5 24.705553999906098 33.96304886726818 23.55966577282904 17.771731359996686 6 18.81428758102568 36.74993760759433 25.138102911857917 19.297671899522072 7 16.785775585295884 19.552157285827 43.3165977835381 20.345469345339012 8 17.81666531475555 23.5290149480193 32.25762384981598 26.396695887409177 9 19.320789882178172 23.04753835804516 33.55327262456228 24.078399135214386 10-11 21.951448070520446 33.12376539592329 23.756466554386467 21.16831997916979 12-13 20.082512580132097 26.854506392641603 30.182224750936715 22.880756276289585 14-15 20.941541513555293 27.778516329260693 28.977722611869115 22.302219545314895 16-17 21.806689660092708 28.228621770414346 27.81681552734957 22.147873042143374 18-19 21.604263178073772 28.417568112674118 27.68787437973475 22.290294329517355 20-21 21.63123245302881 28.406205428364927 27.983874182082296 21.97868793652396 22-23 21.30296173508194 28.752275620034457 28.07343362970428 21.871329015179324 24-25 21.22135601399445 28.63946435034383 28.10579080709374 22.033388828567983 26-27 21.284128499305808 28.549004660435134 28.31667199231967 21.85019484793939 28-29 21.37811534650172 28.493271922363483 28.166383644631825 21.962229086502973 30-31 21.32727647517857 28.43721847495288 28.09021761588879 22.145287433979757 32-33 21.499741800247342 28.618796840582494 27.95624712412173 21.925214235048433 34-35 21.56851364365847 28.565572661261555 27.84913871643699 22.016774978642985 36-37 21.463367639746085 28.523177563120743 27.931596270129834 22.081858527003337 38-39 21.54014487504527 28.593792104310033 28.080210068815646 21.785852951829046 40-41 21.64282990843681 28.582865303179467 28.02221431808858 21.752090470295148 42-43 21.473869359376767 28.42414665527443 28.11175746872064 21.990226516628166 44-45 21.562791068722255 28.43487168927739 28.206408698248925 21.79592854375143 46-47 21.695012339922474 28.489236005034336 27.991809778918647 21.823941876124547 48-49 21.517264591666574 28.43240668227953 28.018711290528785 22.03161743552511 50-51 21.57671341459645 28.396341517601858 28.18238203092882 21.84456303687287 52-53 21.727676199877177 28.569775946416254 27.946496530888236 21.756051322818333 54-55 21.537846565740043 28.402295018159407 28.036303214735632 22.02355520136492 56-57 21.529007180845735 28.37121129800965 28.27875206626119 21.821029454883416 58-59 21.679640403701338 28.508118902445794 28.166525015888034 21.645715677964834 60-61 21.6219783650249 28.295977339528537 28.167103524125658 21.9149407713209 62-63 21.666144052636245 28.296238356793364 28.33269502980541 21.704922560764984 64-65 21.631484675297795 28.503336274011094 28.151869093642446 21.713309957048665 66-67 21.59545754831324 28.44381562332248 28.11038633581707 21.850340492547218 68-69 21.70720172300871 28.478770901925127 28.18458909148147 21.629438283584694 70-71 21.70581925114032 28.548121288286204 28.062847122883028 21.683212337690446 72-73 21.57496398406968 28.422301148019617 28.175111070984165 21.82762379692654 74-75 21.677898749601933 28.343605335619255 28.298210637624422 21.680285277154393 76-77 21.78047811477209 28.425663423998635 28.14598635337487 21.6478721078544 78-79 21.67496034618046 28.287819283571768 28.181049206794484 21.856171163453286 80-81 21.774739405759444 28.360531845177395 28.244145756571125 21.62058299249204 82-83 21.816677352938722 28.447162769424043 28.075330228014195 21.66082964962304 84-85 21.727210830050744 28.257230819230507 28.20329616893808 21.812262181780675 86-87 21.81725138201635 28.411656806599204 28.229365010432506 21.54172680095194 88-89 21.85374232395356 28.46277330993829 28.104191839432914 21.579292526675236 90-91 21.83536633258238 28.34205896865274 28.115045533271093 21.70752916549378 92-93 21.84033473139464 28.378146292697153 28.322469084557934 21.459049891350272 94-95 21.86412613545876 28.449975844808307 28.123732902504393 21.56216511722854 96-97 21.78383353603783 28.373928726095087 28.148062486142194 21.694175251724886 98-99 21.959928266127594 28.39467633741962 28.2305503963049 21.414845000147885 100-101 21.981777177854244 28.551161975200905 27.969679428175915 21.497381418768935 102-103 22.03819897767188 28.448495759764597 27.98102200718914 21.53228325537439 104-105 22.18252942572851 28.49668063108386 27.989460494914926 21.3313294482727 106-107 22.230799932626685 28.577276853646914 27.835272729203194 21.3566504845232 108-109 22.20502199826373 28.539012113495442 27.749855369371218 21.506110518869605 110-111 22.371792320681543 28.68614794083857 27.663430401342097 21.27862933713779 112-113 22.506884618042456 28.84338827213436 27.376989309034723 21.27273780078847 114-115 22.45790499380052 28.845827403295065 27.332745168137972 21.36352243476644 116-117 22.694787484612323 28.914486108028353 27.16348990689833 21.22723650046099 118-119 22.759968402990232 28.97984074103384 26.987349615026048 21.27284124094988 120-121 22.932942862661037 28.998483982656097 26.680074016678724 21.388499138004143 122-123 23.069546500651768 29.121689366549308 26.534330277140043 21.274433855658874 124-125 23.113603278673338 29.245621300336083 26.285161524807776 21.355613896182806 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9.0 1 7.5 2 7.5 3 12.0 4 16.0 5 17.5 6 32.5 7 66.0 8 102.0 9 162.0 10 272.5 11 422.5 12 588.5 13 813.0 14 1102.5 15 1390.5 16 1697.5 17 2050.0 18 2596.5 19 3422.5 20 4452.5 21 5985.5 22 8220.5 23 11560.0 24 16314.5 25 23162.0 26 33468.5 27 48154.5 28 66769.0 29 90591.0 30 123093.0 31 163997.0 32 212356.5 33 270289.0 34 339709.0 35 426132.0 36 528506.0 37 641862.5 38 768536.5 39 910781.5 40 1055837.0 41 1185834.0 42 1290152.0 43 1359906.5 44 1392459.5 45 1377216.0 46 1321454.0 47 1230983.0 48 1101590.0 49 945569.0 50 780543.5 51 634550.0 52 514385.5 53 410147.0 54 314218.0 55 231758.5 56 176611.5 57 140185.0 58 106878.5 59 83817.5 60 69536.0 61 55494.0 62 44572.5 63 34464.0 64 25287.5 65 18854.0 66 15196.0 67 14527.5 68 14035.5 69 12106.0 70 9608.5 71 7024.5 72 6799.0 73 8041.5 74 6478.0 75 5195.5 76 4695.0 77 3412.5 78 2885.5 79 1554.0 80 552.0 81 170.5 82 124.5 83 99.5 84 66.0 85 46.0 86 33.5 87 22.0 88 19.5 89 13.5 90 7.0 91 3.0 92 1.5 93 0.0 94 0.5 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.037362930809478535 2 0.024170337133498383 3 0.0 4 1.5923759740575896E-4 5 0.0 6 3.522528669884971E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0013969480136050674 18-19 2.6298330480648073E-4 20-21 4.1980821134245545E-4 22-23 7.23807260935268E-6 24-25 0.0060269017927209985 26-27 0.038504133590886475 28-29 0.05019844623673062 30-31 0.06756499511743749 32-33 0.0769382991465492 34-35 0.07002835249548718 36-37 0.07489716267071175 38-39 0.0784076278862478 40-41 0.08340189798670115 42-43 0.07762109066269815 44-45 0.07518427288421607 46-47 0.07310694604533186 48-49 0.0742939899532657 50-51 0.09004644864208691 52-53 0.10015079800474325 54-55 0.08994752831642576 56-57 0.6394185723828251 58-59 0.06365161052664747 60-61 0.052367455328666636 62-63 0.03559684109279648 64-65 0.02220881945636381 66-67 0.0015175825570942787 68-69 0.0 70-71 0.0 72-73 0.0030279270415792046 74-75 0.01576693483403992 76-77 0.02341999027299549 78-79 0.03508293793753244 80-81 0.04378551390484414 82-83 0.049498765884493194 84-85 0.04725255068472408 86-87 0.053455578910939335 88-89 0.06731648795784971 90-91 0.04398817993790602 92-93 0.04684239323686076 94-95 0.05216478929560476 96-97 0.086422586955671 98-99 0.07364256341842394 100-101 0.06969298846458717 102-103 0.01402497202605571 104-105 2.653959956762649E-5 106-107 0.016304964898001803 108-109 0.030489174521463273 110-111 0.030392666886671905 112-113 0.041244950418961354 114-115 0.02451293923700774 116-117 0.006767597889744756 118-119 0.001298027687943914 120-121 2.002533421920908E-4 122-123 7.093311157165626E-4 124-125 0.0011822185261942712 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2.0723749E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.71258897899638 #Duplication Level Percentage of deduplicated Percentage of total 1 76.84881553173122 43.582952887737854 2 12.091814010735566 13.715161560026317 3 4.376044743571527 7.44530480687609 4 2.144049542370221 4.863784017881905 5 1.1833311567449858 3.3554886759259364 6 0.7546351659988314 2.567838839903305 7 0.5156736688612498 2.0471632180593398 8 0.379195597939983 1.7204131270892016 9 0.2626382923847976 1.340540777354805 >10 1.3494901062795392 13.263970725103826 >50 0.06490348961125929 2.509254490034802 >100 0.027856011583761664 2.820456197840991 >500 0.001262071744648381 0.4888375086643984 >1k 2.8208506270057364E-4 0.23437084212297282 >5k 8.525379812990087E-6 0.04446232537815351 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.02658785338502218 0.0 0.0 9.650763479136907E-6 0.0 2 0.026684361019813547 1.447614521870536E-5 0.0 1.447614521870536E-5 0.0 3 0.0269063285798337 1.447614521870536E-5 0.0 1.9301526958273814E-5 0.0 4 0.02805476943385099 1.9301526958273814E-5 9.650763479136907E-6 1.9301526958273814E-5 0.0 5 0.028431149209537328 1.9301526958273814E-5 9.650763479136907E-6 2.412690869784227E-5 4.8253817395684535E-6 6 0.029092226507858206 1.9301526958273814E-5 9.650763479136907E-6 2.895229043741072E-5 4.8253817395684535E-6 7 0.029372098648753175 1.9301526958273814E-5 9.650763479136907E-6 2.895229043741072E-5 4.8253817395684535E-6 8 0.029883589113147434 1.9301526958273814E-5 9.650763479136907E-6 2.895229043741072E-5 4.8253817395684535E-6 9 0.030274445034052477 2.412690869784227E-5 9.650763479136907E-6 2.895229043741072E-5 4.8253817395684535E-6 10-11 0.03156523464938704 2.6539599567626495E-5 9.650763479136907E-6 3.860305391654763E-5 4.8253817395684535E-6 12-13 0.03259304095991512 3.136498130719495E-5 9.650763479136907E-6 4.342843565611608E-5 4.8253817395684535E-6 14-15 0.033997227046129536 3.860305391654763E-5 9.650763479136907E-6 4.584112652590031E-5 4.8253817395684535E-6 16-17 0.035227699389719494 4.825381739568453E-5 9.650763479136907E-6 4.825381739568454E-5 4.8253817395684535E-6 18-19 0.03666807583898068 5.307919913525299E-5 9.650763479136907E-6 4.825381739568454E-5 4.8253817395684535E-6 20-21 0.037968516217794374 5.790458087482144E-5 9.650763479136907E-6 5.066650826546877E-5 4.8253817395684535E-6 22-23 0.039297908887045485 5.790458087482144E-5 9.650763479136907E-6 8.685687131223217E-5 4.8253817395684535E-6 24-25 0.04056215890281242 5.790458087482144E-5 9.650763479136907E-6 1.3752337957770094E-4 4.8253817395684535E-6 26-27 0.04185536120901677 5.790458087482144E-5 9.650763479136907E-6 1.544122156661905E-4 4.8253817395684535E-6 28-29 0.0430737700982578 5.790458087482144E-5 9.650763479136907E-6 1.7371374262446432E-4 4.8253817395684535E-6 30-31 0.04442728967620675 6.031727174460567E-5 9.650763479136907E-6 2.0507872393165928E-4 7.23807260935268E-6 32-33 0.045662587401536274 6.27299626143899E-5 9.650763479136907E-6 2.3403101436907E-4 9.650763479136907E-6 34-35 0.04693407548991256 6.514265348417413E-5 9.650763479136907E-6 2.4609446871799116E-4 9.650763479136907E-6 36-37 0.048166960524372304 6.755534435395835E-5 9.650763479136907E-6 2.5574523219712805E-4 9.650763479136907E-6 38-39 0.04964352733668025 6.755534435395835E-5 9.650763479136907E-6 2.605706139366965E-4 9.650763479136907E-6 40-41 0.05103323727767597 8.444418044244794E-5 9.650763479136907E-6 2.702213774158334E-4 9.650763479136907E-6 42-43 0.052543581762160896 1.0133301653093753E-4 9.650763479136907E-6 2.702213774158334E-4 9.650763479136907E-6 44-45 0.05402256126533862 1.1098378001007444E-4 9.650763479136907E-6 2.87110213504323E-4 9.650763479136907E-6 46-47 0.055494302695906994 1.2304723435899558E-4 9.650763479136907E-6 2.9434828611367566E-4 9.650763479136907E-6 48-49 0.05741239193738546 1.3028530696834826E-4 9.650763479136907E-6 3.0158635872302834E-4 9.650763479136907E-6 50-51 0.05932565579712436 1.3993607044748515E-4 9.650763479136907E-6 3.08824431332381E-4 9.650763479136907E-6 52-53 0.061487426816451016 1.616502882755432E-4 9.650763479136907E-6 3.3777672176979175E-4 9.650763479136907E-6 54-55 0.06377707045187625 1.8336450610360124E-4 9.650763479136907E-6 3.7396708481655515E-4 9.650763479136907E-6 56-57 0.06632969739210795 1.8577719697338544E-4 9.650763479136907E-6 3.7637977568633936E-4 9.650763479136907E-6 58-59 0.06916460916410444 2.0025334219209083E-4 9.650763479136907E-6 3.7879246655612357E-4 9.650763479136907E-6 60-61 0.07263888401659371 2.1955486915036464E-4 9.650763479136907E-6 3.8120515742590783E-4 9.650763479136907E-6 62-63 0.07638338024649884 2.2679294175971732E-4 9.650763479136907E-6 3.908559209050447E-4 9.650763479136907E-6 64-65 0.08045600243469461 2.3161832349928577E-4 9.650763479136907E-6 3.9326861177482893E-4 9.650763479136907E-6 66-67 0.08485433789031124 2.4368177784820692E-4 9.650763479136907E-6 4.029193752539659E-4 9.650763479136907E-6 68-69 0.09007298824165454 2.4609446871799116E-4 9.650763479136907E-6 4.077447569935343E-4 9.650763479136907E-6 70-71 0.09651004748223885 2.4850715958777537E-4 9.650763479136907E-6 4.1980821134245545E-4 9.650763479136907E-6 72-73 0.10470837105776566 2.605706139366965E-4 9.650763479136907E-6 4.270462839518082E-4 9.650763479136907E-6 74-75 0.11404065934209104 2.750467591554018E-4 9.650763479136907E-6 4.294589748215924E-4 1.2063454348921133E-5 76-77 0.12479884793045892 2.7745945002518604E-4 9.650763479136907E-6 4.391097383007293E-4 1.447614521870536E-5 78-79 0.1379069974259966 2.9434828611367566E-4 9.650763479136907E-6 4.4876050177986617E-4 1.447614521870536E-5 80-81 0.15572230680848334 3.160625039417337E-4 9.650763479136907E-6 4.511731926496504E-4 1.447614521870536E-5 82-83 0.17971169212674792 3.233005765510864E-4 9.650763479136907E-6 4.6323664699857153E-4 1.447614521870536E-5 84-85 0.21103565768915655 3.3053864916043907E-4 9.650763479136907E-6 4.753001013474927E-4 1.447614521870536E-5 86-87 0.24982931418441712 3.3777672176979175E-4 1.6888836088489588E-5 4.8253817395684537E-4 1.447614521870536E-5 88-89 0.2989613510567031 3.4501479437914443E-4 1.9301526958273814E-5 4.946016283057665E-4 1.447614521870536E-5 90-91 0.3644586700987355 3.5466555785828137E-4 1.9301526958273814E-5 5.114904643942561E-4 1.447614521870536E-5 92-93 0.45517343411175265 3.6914170307698673E-4 1.9301526958273814E-5 5.259666096129615E-4 1.447614521870536E-5 94-95 0.577038449944554 3.8120515742590783E-4 1.9301526958273814E-5 5.259666096129615E-4 1.447614521870536E-5 96-97 0.7288811498344243 3.8120515742590783E-4 1.9301526958273814E-5 5.259666096129615E-4 1.447614521870536E-5 98-99 0.9127836860019873 3.9326861177482893E-4 1.9301526958273814E-5 5.428554457014511E-4 1.447614521870536E-5 100-101 1.148173045330746 4.318716656913766E-4 1.9301526958273814E-5 5.573315909201564E-4 1.447614521870536E-5 102-103 1.4574028087292508 4.6323664699857153E-4 1.9301526958273814E-5 5.64569663529509E-4 1.447614521870536E-5 104-105 1.8551662635944877 4.89776246566198E-4 1.9301526958273814E-5 5.64569663529509E-4 1.447614521870536E-5 106-107 2.322260803293844 5.018397009151191E-4 1.9301526958273814E-5 5.64569663529509E-4 1.447614521870536E-5 108-109 2.8520418771719345 5.283793004827457E-4 1.9301526958273814E-5 5.74220427008646E-4 1.447614521870536E-5 110-111 3.4740745991470945 5.307919913525299E-4 1.9301526958273814E-5 5.886965722273513E-4 1.447614521870536E-5 112-113 4.231963531308934 5.332046822223141E-4 1.9301526958273814E-5 5.983473357064883E-4 1.447614521870536E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTAT 1585 0.0 15.75667 1 TATGCCG 7885 0.0 14.522916 46-47 CGTATGC 9475 0.0 12.461427 44-45 CTCGGCT 6495 0.0 12.267895 1 TGCCGTC 10015 0.0 11.968883 48-49 ACGTCTG 43180 0.0 11.51947 118-119 CCCGTAC 2245 0.0 11.389287 1 CCCGGCT 4225 0.0 11.259204 1 CACGTCT 49595 0.0 11.083923 118-119 CTCGTAC 6215 0.0 11.002747 1 CCCGCAT 4835 0.0 10.945559 1 CTCGGAT 8875 0.0 10.921031 1 ACACGTC 52080 0.0 10.749038 118-119 CGTCTGA 41205 0.0 10.614942 118-119 CGCGGAT 1695 0.0 10.524366 1 CTCCCGT 4500 0.0 10.439002 1 CTCGAAT 9650 0.0 10.413671 1 GCACACG 62550 0.0 10.175409 118-119 CCGTCTT 11655 0.0 10.171499 50-51 CCCGCTT 4825 0.0 10.105574 1 >>END_MODULE skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR3208000 SRR3208000_1.fastq Input file: SRR3208000_1.fastq trimmed: SRR3208000-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Tue Apr 15 04:22:52 2025 >> started Tue Apr 15 04:23:07 2025 >> done (15.195s) 20723749 reads processed; of these: 2354 ( 0.01%) short reads filtered out after trimming by size control 17321 ( 0.08%) empty reads filtered out after trimming by size control 20704074 (99.91%) reads available; of these: 107096 ( 0.52%) trimmed reads available after processing 20596978 (99.48%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 177 0.00% 19 143 0.00% 20 149 0.00% 21 152 0.00% 22 173 0.00% 23 140 0.00% 24 123 0.00% 25 151 0.00% 26 151 0.00% 27 120 0.00% 28 161 0.00% 29 154 0.00% 30 150 0.00% 31 142 0.00% 32 137 0.00% 33 146 0.00% 34 137 0.00% 35 134 0.00% 36 141 0.00% 37 162 0.00% 38 182 0.00% 39 140 0.00% 40 171 0.00% 41 175 0.00% 42 161 0.00% 43 164 0.00% 44 165 0.00% 45 158 0.00% 46 189 0.00% 47 222 0.00% 48 207 0.00% 49 210 0.00% 50 224 0.00% 51 246 0.00% 52 230 0.00% 53 258 0.00% 54 274 0.00% 55 285 0.00% 56 276 0.00% 57 313 0.00% 58 333 0.00% 59 374 0.00% 60 417 0.00% 61 386 0.00% 62 412 0.00% 63 433 0.00% 64 454 0.00% 65 468 0.00% 66 504 0.00% 67 553 0.00% 68 613 0.00% 69 659 0.00% 70 798 0.00% 71 849 0.00% 72 963 0.00% 73 987 0.00% 74 998 0.00% 75 1131 0.01% 76 1275 0.01% 77 1338 0.01% 78 1589 0.01% 79 1872 0.01% 80 2173 0.01% 81 2514 0.01% 82 2890 0.01% 83 3351 0.02% 84 3647 0.02% 85 4056 0.02% 86 4617 0.02% 87 5153 0.02% 88 5813 0.03% 89 6912 0.03% 90 7968 0.04% 91 9523 0.05% 92 11214 0.05% 93 12666 0.06% 94 0 0.00% 95 0 0.00% 96 0 0.00% 97 0 0.00% 98 0 0.00% 99 0 0.00% 100 0 0.00% 101 0 0.00% 102 0 0.00% 103 0 0.00% 104 0 0.00% 105 0 0.00% 106 0 0.00% 107 0 0.00% 108 0 0.00% 109 0 0.00% 110 0 0.00% 111 0 0.00% 112 0 0.00% 113 0 0.00% 114 0 0.00% 115 0 0.00% 116 0 0.00% 117 0 0.00% 118 0 0.00% 119 0 0.00% 120 0 0.00% 121 0 0.00% 122 0 0.00% 123 0 0.00% 124 0 0.00% 125 20596978 99.48% 20704074 reads passed initial QC criterion=sequence-density sequence-density=3.70 sequence-density-rank=1 fanout-score=44.32 fanout-score-rank=1 prefix-density=5.14 prefix-fanout=31.9 sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC criterion=fanout-score sequence-density=3.70 sequence-density-rank=1 fanout-score=44.32 fanout-score-rank=1 prefix-density=5.14 prefix-fanout=31.9 sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC Potential 3prime adapter identified. Now checking if in reference sequence Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192. 1 reads; of these: 1 (100.00%) were unpaired; of these: 1 (100.00%) aligned 0 times 0 (0.00%) aligned exactly 1 time 0 (0.00%) aligned >1 times 0.00% overall alignment rate Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC -o SRR3208000 - Input file: STDIN trimmed: SRR3208000-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): 3 -- number of concurrent threads (-t): 20 Tue Apr 15 04:24:03 2025 >> started Tue Apr 15 04:24:13 2025 >> done (10.869s) 10352037 reads processed; of these: 110 ( 0.00%) short reads filtered out after trimming by size control 208 ( 0.00%) empty reads filtered out after trimming by size control 10351719 (100.00%) reads available; of these: 1293537 (12.50%) trimmed reads available after processing 9058182 (87.50%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 91 0.00% 19 68 0.00% 20 77 0.00% 21 93 0.00% 22 80 0.00% 23 85 0.00% 24 68 0.00% 25 76 0.00% 26 89 0.00% 27 55 0.00% 28 88 0.00% 29 86 0.00% 30 74 0.00% 31 68 0.00% 32 78 0.00% 33 83 0.00% 34 80 0.00% 35 69 0.00% 36 74 0.00% 37 87 0.00% 38 88 0.00% 39 76 0.00% 40 79 0.00% 41 85 0.00% 42 79 0.00% 43 81 0.00% 44 85 0.00% 45 76 0.00% 46 122 0.00% 47 111 0.00% 48 98 0.00% 49 106 0.00% 50 105 0.00% 51 138 0.00% 52 135 0.00% 53 129 0.00% 54 141 0.00% 55 154 0.00% 56 156 0.00% 57 164 0.00% 58 161 0.00% 59 188 0.00% 60 208 0.00% 61 181 0.00% 62 228 0.00% 63 203 0.00% 64 234 0.00% 65 238 0.00% 66 242 0.00% 67 307 0.00% 68 316 0.00% 69 339 0.00% 70 400 0.00% 71 396 0.00% 72 433 0.00% 73 486 0.00% 74 550 0.01% 75 572 0.01% 76 648 0.01% 77 677 0.01% 78 827 0.01% 79 978 0.01% 80 1114 0.01% 81 1265 0.01% 82 1497 0.01% 83 1712 0.02% 84 1836 0.02% 85 2060 0.02% 86 2352 0.02% 87 2594 0.03% 88 2922 0.03% 89 3459 0.03% 90 3966 0.04% 91 4638 0.04% 92 5501 0.05% 93 6310 0.06% 94 7328 0.07% 95 8095 0.08% 96 8839 0.09% 97 9731 0.09% 98 10779 0.10% 99 12524 0.12% 100 14099 0.14% 101 16434 0.16% 102 18605 0.18% 103 21036 0.20% 104 23406 0.23% 105 24661 0.24% 106 26434 0.26% 107 27826 0.27% 108 30036 0.29% 109 33028 0.32% 110 36051 0.35% 111 38872 0.38% 112 43248 0.42% 113 46575 0.45% 114 50279 0.49% 115 52722 0.51% 116 55229 0.53% 117 56291 0.54% 118 58164 0.56% 119 62531 0.60% 120 76399 0.74% 121 118342 1.14% 122 304685 2.94% 123 0 0.00% 124 0 0.00% 125 9005655 87.00% criterion=sequence-density sequence-density=0.08 sequence-density-rank=1 fanout-score=4.31 fanout-score-rank=24 prefix-density=0.11 prefix-fanout=3.0 sequence=TTCAACCAAGCGCG criterion=fanout-score sequence-density=0.05 sequence-density-rank=14 fanout-score=326.57 fanout-score-rank=1 prefix-density=0.49 prefix-fanout=30.4 sequence=TTCTTCTTCTTC Started job on | Apr 15 04:24:41 Started mapping on | Apr 15 04:24:42 Finished on | Apr 15 04:25:16 Mapping speed, Million of reads per hour | 2192.16 Number of input reads | 20703756 Average input read length | 124 UNIQUE READS: Uniquely mapped reads number | 18865074 Uniquely mapped reads % | 91.12% Average mapped length | 123.21 Number of splices: Total | 7110804 Number of splices: Annotated (sjdb) | 6968768 Number of splices: GT/AG | 6998906 Number of splices: GC/AG | 91656 Number of splices: AT/AC | 6840 Number of splices: Non-canonical | 13402 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.02% Deletion average length | 2.21 Insertion rate per base | 0.02% Insertion average length | 1.59 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 415495 % of reads mapped to multiple loci | 2.01% Number of reads mapped to too many loci | 771056 % of reads mapped to too many loci | 3.72% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.12% % of reads unmapped: other | 0.03% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1423187 1423187 1423187 N_multimapping 415495 415495 415495 N_noFeature 895708 9806021 9833652 N_ambiguous 192707 36024 35950 UnstrandedReadsAssigned:17776659 PositiveStrandReadsAssigned:9023029 NegativeStrandReadsAssigned:8995472 Dataset is classified unstranded MeadianReadLen=125 20thPercentileLength=125 echo kmer=121 SRR3208000 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR3208000-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,703,756 reads, 18,791,713 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,235 rounds 52401 SRR3208000.ke.tsv 34699 SRR3208000.se.tsv 87100 total ==> SRR3208000.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 694 27.0396 Potri.005G024800.1.v4.1 1035 936 141 11.2631 Potri.004G059700.1.v4.1 961 862 26 2.25518 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 260.25 6.8419 Potri.016G087400.1.v4.1 270 171 766 334.925 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 91.5517 4.08909 Potri.012G127500.1.v4.1 977 878 3886 330.92 ==> SRR3208000.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 2308 Potri.001G233950.v4.1 1 Potri.001G122700.v4.1 369 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 27 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 1 Potri.001G452600.v4.1 6 SRR3208000 completed mapping pipeline successfully