Starting /dee2/code/volunteer_pipeline.sh SRR5423381 current disk space = 3051664592896 free memory = 1578079240 SRR5423381 SRAfilesize 28c77076ca47d1d046c7a1c76c764611 SRR5423381.sra SRR5423381.sra file validated SRR5423381 is single end SRR5423381 is conventional basespace SRR5423381 read1 length is 52 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5423381_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.967 34.0 31.0 34.0 25.0 34.0 2 31.30875 34.0 31.0 34.0 26.0 34.0 3 32.24575 34.0 31.0 34.0 28.0 34.0 4 35.93625 37.0 35.0 37.0 35.0 37.0 5 36.0305 37.0 35.0 37.0 35.0 37.0 6 35.99925 37.0 35.0 37.0 35.0 37.0 7 36.01375 37.0 35.0 37.0 35.0 37.0 8 36.023 37.0 35.0 37.0 35.0 37.0 9 37.85525 39.0 38.0 39.0 35.0 39.0 10 37.76675 39.0 38.0 39.0 35.0 39.0 11 37.8335 39.0 38.0 39.0 35.0 39.0 12 37.81225 39.0 38.0 39.0 35.0 39.0 13 37.70525 39.0 38.0 39.0 35.0 39.0 14 39.1785 41.0 39.0 41.0 36.0 41.0 15 39.16575 40.0 39.0 41.0 36.0 41.0 16 39.14575 40.0 39.0 41.0 36.0 41.0 17 39.13525 40.0 39.0 41.0 36.0 41.0 18 39.10425 40.0 38.0 41.0 36.0 41.0 19 39.08525 40.0 39.0 41.0 36.0 41.0 20 39.01325 40.0 39.0 41.0 36.0 41.0 21 38.8905 40.0 38.0 41.0 35.0 41.0 22 38.91075 40.0 39.0 41.0 35.0 41.0 23 38.7705 40.0 38.0 41.0 35.0 41.0 24 38.82475 40.0 38.0 41.0 35.0 41.0 25 38.74475 40.0 38.0 41.0 34.0 41.0 26 38.61325 40.0 38.0 41.0 34.0 41.0 27 38.54725 40.0 38.0 41.0 34.0 41.0 28 38.414 40.0 38.0 41.0 34.0 41.0 29 38.43175 40.0 38.0 41.0 34.0 41.0 30 38.38175 40.0 38.0 41.0 34.0 41.0 31 38.2985 40.0 38.0 41.0 34.0 41.0 32 37.98675 40.0 38.0 41.0 33.0 41.0 33 37.953 40.0 38.0 41.0 33.0 41.0 34 37.937 40.0 38.0 41.0 33.0 41.0 35 38.03025 40.0 38.0 41.0 33.0 41.0 36 37.7375 40.0 38.0 41.0 32.0 41.0 37 37.6435 40.0 38.0 41.0 32.0 41.0 38 37.47075 40.0 38.0 41.0 31.0 41.0 39 37.438 40.0 37.0 41.0 31.0 41.0 40 37.55625 40.0 38.0 41.0 32.0 41.0 41 37.53375 40.0 38.0 41.0 31.0 41.0 42 37.35375 40.0 37.0 41.0 31.0 41.0 43 37.04675 40.0 37.0 41.0 30.0 41.0 44 37.03525 40.0 37.0 41.0 30.0 41.0 45 36.87425 40.0 36.0 41.0 30.0 41.0 46 36.83575 40.0 36.0 41.0 30.0 41.0 47 36.5055 40.0 36.0 41.0 28.0 41.0 48 36.448 39.0 36.0 41.0 28.0 41.0 49 36.6385 40.0 36.0 41.0 28.0 41.0 50 36.5505 40.0 36.0 41.0 28.0 41.0 51 36.4405 40.0 35.0 41.0 28.0 41.0 52 34.254 38.0 33.0 40.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10 0.0 1101 11 0.0 1101 12 0.0 1101 13 0.0 1101 14 0.0 1101 15 0.0 1101 16 0.0 1101 17 0.0 1101 18 0.0 1101 19 0.0 1101 20 0.0 1101 21 0.0 1101 22 0.0 1101 23 0.0 1101 24 0.0 1101 25 0.0 1101 26 0.0 1101 27 0.0 1101 28 0.0 1101 29 0.0 1101 30 0.0 1101 31 0.0 1101 32 0.0 1101 33 0.0 1101 34 0.0 1101 35 0.0 1101 36 0.0 1101 37 0.0 1101 38 0.0 1101 39 0.0 1101 40 0.0 1101 41 0.0 1101 42 0.0 1101 43 0.0 1101 44 0.0 1101 45 0.0 1101 46 0.0 1101 47 0.0 1101 48 0.0 1101 49 0.0 1101 50 0.0 1101 51 0.0 1101 52 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 1.0 11 1.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 2.0 20 5.0 21 0.0 22 6.0 23 12.0 24 14.0 25 15.0 26 23.0 27 28.0 28 35.0 29 47.0 30 47.0 31 85.0 32 83.0 33 107.0 34 161.0 35 206.0 36 282.0 37 423.0 38 765.0 39 1646.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.49169163715609 12.367202397166984 5.475347316807409 36.66575864886952 2 19.875 14.475 35.5 30.15 3 19.6 19.825 25.575 35.0 4 24.075 28.775000000000002 21.525 25.624999999999996 5 24.6 32.65 23.275000000000002 19.475 6 20.525 34.175 22.650000000000002 22.650000000000002 7 16.325 24.05 39.7 19.925 8 17.075000000000003 23.225 30.225 29.475 9 19.5 21.575 32.65 26.275 10 19.075 38.925 23.05 18.95 11 23.425 28.849999999999998 21.65 26.075 12 23.025000000000002 25.374999999999996 24.5 27.1 13 20.125 28.475 26.650000000000002 24.75 14 18.95 29.025000000000002 27.925 24.099999999999998 15 20.325 27.85 27.450000000000003 24.375 16 20.775 28.175 26.224999999999998 24.825 17 20.75 26.974999999999998 26.450000000000003 25.825 18 20.65 27.825 26.150000000000002 25.374999999999996 19 23.200000000000003 26.825 25.074999999999996 24.9 20 20.474999999999998 25.95 28.225 25.35 21 21.0 26.474999999999998 24.9 27.625 22 19.875 29.025000000000002 24.2 26.900000000000002 23 21.4 29.025000000000002 24.45 25.124999999999996 24 22.525000000000002 26.200000000000003 26.950000000000003 24.325 25 21.475 26.674999999999997 26.025 25.825 26 20.95 27.3 26.224999999999998 25.525 27 20.549999999999997 27.375 26.85 25.224999999999998 28 22.25 27.85 25.2 24.7 29 20.95 26.525 28.225 24.3 30 20.200000000000003 25.05 26.55 28.199999999999996 31 21.0 27.900000000000002 25.174999999999997 25.924999999999997 32 21.075 27.200000000000003 26.55 25.174999999999997 33 21.05 25.424999999999997 27.775 25.75 34 20.9 26.5 26.05 26.55 35 21.425 26.950000000000003 25.25 26.375 36 20.4 26.724999999999998 24.925 27.950000000000003 37 22.05 27.224999999999998 25.374999999999996 25.35 38 21.6 26.825 24.4 27.175 39 21.349999999999998 26.075 25.3 27.275 40 21.925 27.750000000000004 25.324999999999996 25.0 41 21.575 26.85 25.650000000000002 25.924999999999997 42 21.099999999999998 26.25 25.674999999999997 26.974999999999998 43 22.15 26.8 25.05 26.0 44 22.05 26.875 24.7 26.375 45 23.5 26.5 25.2 24.8 46 25.15 25.424999999999997 24.3 25.124999999999996 47 23.849999999999998 27.450000000000003 24.9 23.799999999999997 48 21.9 27.950000000000003 24.325 25.825 49 23.0 26.325 24.525 26.150000000000002 50 21.4 27.150000000000002 25.525 25.924999999999997 51 22.900000000000002 25.224999999999998 23.35 28.525 52 22.55 27.3 23.575 26.575 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2.0 1 1.5 2 1.0 3 2.0 4 3.0 5 1.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 2.0 16 2.0 17 2.0 18 3.0 19 4.0 20 9.5 21 15.0 22 13.5 23 12.0 24 15.5 25 19.0 26 22.5 27 26.0 28 37.0 29 48.0 30 56.5 31 65.0 32 82.5 33 100.0 34 114.5 35 129.0 36 154.5 37 180.0 38 196.0 39 223.5 40 235.0 41 227.5 42 220.0 43 244.5 44 269.0 45 287.0 46 305.0 47 313.5 48 322.0 49 323.0 50 324.0 51 315.0 52 306.0 53 295.0 54 284.0 55 262.0 56 240.0 57 221.0 58 202.0 59 170.0 60 138.0 61 133.0 62 128.0 63 99.0 64 58.5 65 47.0 66 43.0 67 39.0 68 28.0 69 17.0 70 14.0 71 11.0 72 10.5 73 10.0 74 6.5 75 3.0 76 3.5 77 4.0 78 4.0 79 4.0 80 2.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 8.225 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.625 #Duplication Level Percentage of deduplicated Percentage of total 1 93.80753138075313 84.075 2 3.933054393305439 7.049999999999999 3 1.0878661087866108 2.9250000000000003 4 0.502092050209205 1.7999999999999998 5 0.2510460251046025 1.125 6 0.22315202231520223 1.2 7 0.0 0.0 8 0.0 0.0 9 0.11157601115760112 0.8999999999999999 >10 0.08368200836820083 0.9249999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCAGACTAAGCAATAAAGTACCTCGTATTTTCCCCTCTGCCTCGGGTTGTC 17 0.42500000000000004 No Hit CCCAGGTATTAATTACACGACCTTGACTATCAACTACAGATTGGTTGAAATT 10 0.25 No Hit GCCTAACATGTGAAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACA 10 0.25 No Hit GTCCCTTATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCA 9 0.22499999999999998 No Hit GGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGA 9 0.22499999999999998 No Hit GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGTA 9 0.22499999999999998 No Hit AGCACGGTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCA 9 0.22499999999999998 No Hit CTCACGAGCAAGATCACGTCCCTCATTACGAGCTTGTACACATGCTTCTAGA 6 0.15 No Hit GTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCAACTACA 6 0.15 No Hit GGGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCG 6 0.15 No Hit CAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACTGGT 6 0.15 No Hit GCCTCCTCGAGCTCAATCAGCACCTGAGATGCCTCAGTGCATCCAAACATGG 6 0.15 No Hit CGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGC 6 0.15 No Hit GTCGGTCCATGGATTTTCCTTCCTTTTTCCGCATTTCGCTAAAGGGTTGAAG 6 0.15 No Hit GCCGAATACACCAGCTACGCCTAACATGTGAAATGGGTGCATAAGGATGTTG 6 0.15 No Hit GTGCTGAGTTGGAATCCCATTCTAACTAAGGATTCTTGTGGTTCCGGAGGAT 5 0.125 No Hit GTCGAATCCAATGATACGGATAAAGGAGTTAGGGTAAGCTTTCTTCGCCTCC 5 0.125 No Hit CCAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACTGG 5 0.125 No Hit CTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGAGGCATCCCA 5 0.125 No Hit CCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACT 5 0.125 No Hit GCACGGTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCAA 5 0.125 No Hit GTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCC 5 0.125 No Hit ATCAAACTAGAGGTTACCAAAGAACCATGCATAGCACTGAATAGGGAGCCGC 5 0.125 No Hit GTTAAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACCATGAGCGGCT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra Read 200000 spots for SRR5423381.sra Written 200000 spots for SRR5423381.sra SRR ids: ['SRR5423381.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ukl5dmkm SRR5423381.sra spots: 4000000 blocks: [[1, 200000], [200001, 400000], [400001, 600000], [600001, 800000], [800001, 1000000], [1000001, 1200000], [1200001, 1400000], [1400001, 1600000], [1600001, 1800000], [1800001, 2000000], [2000001, 2200000], [2200001, 2400000], [2400001, 2600000], [2600001, 2800000], [2800001, 3000000], [3000001, 3200000], [3200001, 3400000], [3400001, 3600000], [3600001, 3800000], [3800001, 4000000]] SRR5423381 file size 703976 SRR5423381 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423381 SRR5423381_1.fastq Input file: SRR5423381_1.fastq trimmed: SRR5423381-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Thu Feb 13 04:38:27 2025 >> started Thu Feb 13 04:46:43 2025 >> done (495.976s) 4000000 reads processed; of these: 84 ( 0.00%) short reads filtered out after trimming by size control 904 ( 0.02%) empty reads filtered out after trimming by size control 3999012 (99.98%) reads available; of these: 103201 ( 2.58%) trimmed reads available after processing 3895811 (97.42%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 8 0.00% 20 7 0.00% 21 3 0.00% 22 2 0.00% 23 6 0.00% 24 6 0.00% 25 8 0.00% 26 3 0.00% 27 9 0.00% 28 7 0.00% 29 13 0.00% 30 13 0.00% 31 16 0.00% 32 18 0.00% 33 34 0.00% 34 28 0.00% 35 32 0.00% 36 41 0.00% 37 40 0.00% 38 67 0.00% 39 69 0.00% 40 96 0.00% 41 128 0.00% 42 153 0.00% 43 221 0.01% 44 360 0.01% 45 508 0.01% 46 656 0.02% 47 1062 0.03% 48 1937 0.05% 49 4212 0.11% 50 12635 0.32% 51 80797 2.02% 52 3895811 97.42% 3999012 reads passed initial QC criterion=sequence-density sequence-density=0.38 sequence-density-rank=1 fanout-score=1.91 fanout-score-rank=26 prefix-density=0.37 prefix-fanout=1.9 sequence=TGGCACCGGCCCGTAATCCTTCCACTGCCAGGAGCGGGAGGTGATCGCTGCTCTGTGAGACCAGCCTCACGCTGTGCCCTCCCCTCTAGGAGCCACGACACC criterion=fanout-score sequence-density=0.01 sequence-density-rank=40 fanout-score=19.42 fanout-score-rank=1 prefix-density=0.06 prefix-fanout=1.7 sequence=TGGGCCGAGTTTAATTTAATTGCAATTCAATTACGAGAATGAACATTAATTAGTGGATTACAACGTATCCATTGCTTGGAATTCAAATTTAATCTCCTTCCATACTTCACAAGCAGCAGCTAGTTCAGGGCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGATCACGTCCCTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAACGGCACCGGGTGCATTTCCCCAAGGGTGCCCTAAAGTTCCTCCACCGAATTGTAGTACGGAATCATCTCCAAAGATCTCGGTCAGAGCAGGCATATGCCAAACGTGAATACCCCCCGAAGCCACGGGTAGAACACCCGGTAGAGAGACCCAATCTTGAGTGAAATAAATACCGCGGCTTCGATCTTTTTCAACAAAATCATCACGCAGTAAATCAACAAAACCCAAAGTTATGTCTCTTTCCCCTTCAAGTTTACCTACTACGGTACCAGAGTGAATA Started job on | Feb 13 04:53:10 Started mapping on | Feb 13 04:53:26 Finished on | Feb 13 05:10:40 Mapping speed, Million of reads per hour | 13.92 Number of input reads | 3999012 Average input read length | 51 UNIQUE READS: Uniquely mapped reads number | 3001927 Uniquely mapped reads % | 75.07% Average mapped length | 51.79 Number of splices: Total | 258113 Number of splices: Annotated (sjdb) | 254706 Number of splices: GT/AG | 250182 Number of splices: GC/AG | 6382 Number of splices: AT/AC | 899 Number of splices: Non-canonical | 650 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.01% Deletion average length | 2.34 Insertion rate per base | 0.00% Insertion average length | 1.33 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 846619 % of reads mapped to multiple loci | 21.17% Number of reads mapped to too many loci | 73022 % of reads mapped to too many loci | 1.83% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.82% % of reads unmapped: other | 0.12% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 150466 150466 150466 N_multimapping 846619 846619 846619 N_noFeature 452923 2931686 512300 N_ambiguous 22242 153 11242 UnstrandedReadsAssigned:2526762 PositiveStrandReadsAssigned:70088 NegativeStrandReadsAssigned:2478385 Dataset is classified negative stranded MeadianReadLen=52 20thPercentileLength=52 echo kmer=47 SRR5423381 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR5423381-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,999,012 reads, 3,156,055 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,094 rounds 52401 SRR5423381.ke.tsv 34699 SRR5423381.se.tsv 87100 total ==> SRR5423381.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 76 10.5837 Potri.005G024800.1.v4.1 1035 936 6 1.71306 Potri.004G059700.1.v4.1 961 862 5 1.5501 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 74.0369 6.95691 Potri.016G087400.1.v4.1 270 171 19 29.6931 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 1 0.15964 Potri.012G127500.1.v4.1 977 878 6 1.82622 ==> SRR5423381.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 5 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 21 Potri.001G212900.v4.1 2 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 4 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR5423381 completed mapping pipeline successfully