Starting /dee2/code/volunteer_pipeline.sh SRR5423387 current disk space = 3049641013248 free memory = 1579266120 SRR5423387 SRAfilesize 8f0397aa08a52bb86dc5c9e79db10fea SRR5423387.sra SRR5423387.sra file validated SRR5423387 is single end SRR5423387 is conventional basespace SRR5423387 read1 length is 52 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5423387_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.59625 34.0 31.0 34.0 31.0 34.0 2 32.74325 34.0 31.0 34.0 31.0 34.0 3 32.77975 34.0 31.0 34.0 31.0 34.0 4 36.0965 37.0 37.0 37.0 35.0 37.0 5 36.10975 37.0 37.0 37.0 35.0 37.0 6 36.1615 37.0 37.0 37.0 35.0 37.0 7 36.12325 37.0 36.0 37.0 35.0 37.0 8 36.12825 37.0 36.0 37.0 35.0 37.0 9 37.94075 39.0 38.0 39.0 35.0 39.0 10 37.84725 39.0 38.0 39.0 35.0 39.0 11 37.8945 39.0 38.0 39.0 35.0 39.0 12 37.814 39.0 38.0 39.0 35.0 39.0 13 37.8745 39.0 38.0 39.0 35.0 39.0 14 39.213 41.0 39.0 41.0 36.0 41.0 15 39.22625 41.0 39.0 41.0 36.0 41.0 16 39.235 41.0 39.0 41.0 36.0 41.0 17 39.05025 41.0 39.0 41.0 36.0 41.0 18 39.11225 41.0 39.0 41.0 36.0 41.0 19 39.15725 41.0 39.0 41.0 36.0 41.0 20 39.105 40.0 39.0 41.0 36.0 41.0 21 39.11575 40.0 39.0 41.0 36.0 41.0 22 38.96275 40.0 39.0 41.0 35.0 41.0 23 39.15125 40.0 39.0 41.0 36.0 41.0 24 39.059 40.0 39.0 41.0 35.0 41.0 25 38.9235 40.0 39.0 41.0 35.0 41.0 26 38.83525 40.0 38.0 41.0 35.0 41.0 27 38.787 40.0 38.0 41.0 35.0 41.0 28 38.7675 40.0 38.0 41.0 35.0 41.0 29 38.84575 40.0 38.0 41.0 35.0 41.0 30 38.86875 40.0 38.0 41.0 35.0 41.0 31 38.76025 40.0 38.0 41.0 35.0 41.0 32 38.729 40.0 38.0 41.0 35.0 41.0 33 38.61 40.0 38.0 41.0 34.0 41.0 34 38.55225 40.0 38.0 41.0 34.0 41.0 35 38.56225 40.0 38.0 41.0 34.0 41.0 36 38.4935 40.0 38.0 41.0 34.0 41.0 37 38.31875 40.0 38.0 41.0 34.0 41.0 38 38.25425 40.0 38.0 41.0 33.0 41.0 39 38.165 40.0 38.0 41.0 33.0 41.0 40 38.1145 40.0 38.0 41.0 33.0 41.0 41 38.07125 40.0 38.0 41.0 33.0 41.0 42 38.05425 40.0 38.0 41.0 33.0 41.0 43 37.7845 40.0 38.0 41.0 33.0 41.0 44 37.79625 40.0 38.0 41.0 33.0 41.0 45 37.69275 40.0 37.0 41.0 32.0 41.0 46 37.4855 40.0 37.0 41.0 32.0 41.0 47 37.56075 40.0 37.0 41.0 32.0 41.0 48 37.64825 40.0 37.0 41.0 32.0 41.0 49 37.467 40.0 37.0 41.0 31.0 41.0 50 37.30175 40.0 37.0 41.0 31.0 41.0 51 37.2275 40.0 37.0 41.0 31.0 41.0 52 35.804 38.0 35.0 40.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2301 1 0.0 2301 2 0.0 2301 3 0.0 2301 4 0.0 2301 5 0.0 2301 6 0.0 2301 7 0.0 2301 8 0.0 2301 9 0.0 2301 10 0.0 2301 11 0.0 2301 12 0.0 2301 13 0.0 2301 14 0.0 2301 15 0.0 2301 16 0.0 2301 17 0.0 2301 18 0.0 2301 19 0.0 2301 20 0.0 2301 21 0.0 2301 22 0.0 2301 23 0.0 2301 24 0.0 2301 25 0.0 2301 26 0.0 2301 27 0.0 2301 28 0.0 2301 29 0.0 2301 30 0.0 2301 31 0.0 2301 32 0.0 2301 33 0.0 2301 34 0.0 2301 35 0.0 2301 36 0.0 2301 37 0.0 2301 38 0.0 2301 39 0.0 2301 40 0.0 2301 41 0.0 2301 42 0.0 2301 43 0.0 2301 44 0.0 2301 45 0.0 2301 46 0.0 2301 47 0.0 2301 48 0.0 2301 49 0.0 2301 50 0.0 2301 51 0.0 2301 52 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 1.0 12 0.0 13 1.0 14 0.0 15 0.0 16 0.0 17 2.0 18 0.0 19 0.0 20 2.0 21 2.0 22 5.0 23 4.0 24 13.0 25 13.0 26 8.0 27 18.0 28 20.0 29 35.0 30 45.0 31 55.0 32 55.0 33 83.0 34 140.0 35 163.0 36 254.0 37 360.0 38 711.0 39 1999.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.04106159238858 13.520280420630945 6.184276414621933 36.25438157235854 2 20.8 15.6 35.675000000000004 27.925 3 19.55 19.325 25.05 36.075 4 25.45 27.474999999999998 21.55 25.525 5 24.325 34.225 21.7 19.75 6 20.5 34.65 22.75 22.1 7 15.925 24.224999999999998 40.550000000000004 19.3 8 17.525 23.125 30.075000000000003 29.275000000000002 9 18.275 22.175 32.7 26.85 10 20.075000000000003 38.45 22.525000000000002 18.95 11 22.7 28.925 21.3 27.075 12 20.849999999999998 24.525 24.825 29.799999999999997 13 21.0 28.349999999999998 26.5 24.15 14 20.200000000000003 28.675 27.125 24.0 15 20.125 27.3 26.575 26.0 16 21.075 27.325 26.35 25.25 17 22.075 26.25 25.924999999999997 25.75 18 21.5 26.650000000000002 25.45 26.400000000000002 19 21.675 28.15 25.674999999999997 24.5 20 20.65 27.175 27.525 24.65 21 20.424999999999997 26.125 26.900000000000002 26.55 22 22.075 28.125 24.349999999999998 25.45 23 21.325 27.875 24.95 25.85 24 23.150000000000002 26.724999999999998 24.525 25.6 25 21.575 28.050000000000004 24.7 25.674999999999997 26 22.825 27.05 26.174999999999997 23.95 27 22.075 26.625 25.45 25.85 28 22.775000000000002 27.425 26.224999999999998 23.575 29 21.125 27.900000000000002 26.8 24.175 30 20.9 25.95 24.95 28.199999999999996 31 21.25 28.299999999999997 25.4 25.05 32 22.25 27.725 25.35 24.675 33 22.225 25.324999999999996 26.224999999999998 26.224999999999998 34 20.575 26.974999999999998 25.575 26.875 35 20.25 26.950000000000003 25.575 27.224999999999998 36 21.4 27.224999999999998 24.575 26.8 37 21.45 26.85 25.85 25.85 38 21.0 27.3 24.65 27.05 39 21.75 26.974999999999998 24.95 26.325 40 21.349999999999998 28.475 25.35 24.825 41 22.075 27.275 24.725 25.924999999999997 42 21.05 25.275 27.325 26.35 43 23.1 27.05 25.474999999999998 24.375 44 23.375 27.0 25.474999999999998 24.15 45 22.225 25.8 24.375 27.6 46 22.0 27.775 25.0 25.224999999999998 47 22.75 27.375 24.975 24.9 48 23.599999999999998 26.974999999999998 24.275 25.15 49 22.650000000000002 27.425 25.474999999999998 24.45 50 21.875 27.6 25.15 25.374999999999996 51 22.325 26.400000000000002 24.2 27.075 52 23.175 27.575 24.7 24.55 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 1.0 2 1.0 3 1.0 4 1.0 5 0.5 6 0.0 7 2.0 8 4.0 9 2.5 10 1.0 11 0.5 12 0.0 13 0.5 14 1.5 15 2.0 16 2.0 17 2.0 18 5.5 19 9.0 20 7.0 21 5.0 22 11.5 23 18.0 24 17.0 25 16.0 26 20.0 27 24.0 28 34.0 29 44.0 30 46.5 31 49.0 32 77.0 33 105.0 34 115.0 35 125.0 36 139.5 37 154.0 38 171.0 39 227.5 40 267.0 41 256.0 42 245.0 43 255.5 44 266.0 45 285.0 46 304.0 47 305.5 48 307.0 49 318.0 50 329.0 51 332.5 52 336.0 53 322.5 54 309.0 55 278.5 56 248.0 57 214.5 58 181.0 59 165.5 60 150.0 61 123.0 62 96.0 63 84.0 64 65.0 65 58.0 66 46.0 67 34.0 68 24.0 69 14.0 70 13.5 71 13.0 72 8.5 73 4.0 74 4.0 75 4.0 76 4.5 77 5.0 78 3.5 79 2.0 80 1.5 81 1.0 82 1.5 83 2.0 84 1.0 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 1.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.75 #Duplication Level Percentage of deduplicated Percentage of total 1 92.96296296296296 81.575 2 4.216524216524217 7.3999999999999995 3 1.225071225071225 3.225 4 0.7122507122507122 2.5 5 0.2849002849002849 1.25 6 0.2564102564102564 1.35 7 0.08547008547008547 0.525 8 0.11396011396011395 0.8 9 0.028490028490028487 0.22499999999999998 >10 0.11396011396011395 1.15 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCCTTATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCA 13 0.325 No Hit GCACGGTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCAA 12 0.3 No Hit CCCTGATCAAACTAGAGGTTACCAAAGAACCATGCATAGCACTGAATAGGGA 11 0.27499999999999997 No Hit CCAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACTGG 10 0.25 No Hit CTCCAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACT 9 0.22499999999999998 No Hit CTCGTATTTTCCCCTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTG 8 0.2 No Hit GTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCAACTACA 8 0.2 No Hit GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGTA 8 0.2 No Hit GCCGAATACACCAGCTACGCCTAACATGTGAAATGGGTGCATAAGGATGTTG 8 0.2 No Hit GCCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTAC 7 0.17500000000000002 No Hit CCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACT 7 0.17500000000000002 No Hit CTCAGCCTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGAGGC 7 0.17500000000000002 No Hit GGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGT 6 0.15 No Hit GTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCCCTGCTTCCTCGG 6 0.15 No Hit GATCAAACTAGAGGTTACCAAAGAACCATGCATAGCACTGAATAGGGAGCCG 6 0.15 No Hit GTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGA 6 0.15 No Hit GCCAACTTGATCCTCTTCCCCAGGGATCCCAGATGAGGGAACCCTAGGAGAG 6 0.15 No Hit GCCTCCTCGAGCTCAATCAGCACCTGAGATGCCTCAGTGCATCCAAACATGG 6 0.15 No Hit GGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGA 6 0.15 No Hit GTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCC 6 0.15 No Hit ATCAAACTAGAGGTTACCAAAGAACCATGCATAGCACTGAATAGGGAGCCGC 6 0.15 No Hit GCTCAGCCTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGAGG 5 0.125 No Hit CCCAGGTATTAATTACACGACCTTGACTATCAACTACAGATTGGTTGAAATT 5 0.125 No Hit GTTCTATGGTCGGTCCGCGACCCCTGGATGCCGAAGGCGTCCTTGGGGCGAT 5 0.125 No Hit AATTACACGACCTTGACTATCAACTACAGATTGGTTGAAATTGAAACCATTT 5 0.125 No Hit CCGTAATCCTTCCACTGCCAGGAGCGGGAGGTGATCGCTGCTCTGTGAGACC 5 0.125 No Hit TAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCAACTACAGA 5 0.125 No Hit CGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGC 5 0.125 No Hit CCGCATTTCGCTAAAGGGTTGAAGGAGGATAGTGCATCAAGCTGTTCGCAAG 5 0.125 No Hit GCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGATCA 5 0.125 No Hit AGCACGGTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra Read 200000 spots for SRR5423387.sra Written 200000 spots for SRR5423387.sra SRR ids: ['SRR5423387.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_t847m9oo SRR5423387.sra spots: 4000000 blocks: [[1, 200000], [200001, 400000], [400001, 600000], [600001, 800000], [800001, 1000000], [1000001, 1200000], [1200001, 1400000], [1400001, 1600000], [1600001, 1800000], [1800001, 2000000], [2000001, 2200000], [2200001, 2400000], [2400001, 2600000], [2600001, 2800000], [2800001, 3000000], [3000001, 3200000], [3200001, 3400000], [3400001, 3600000], [3600001, 3800000], [3800001, 4000000]] SRR5423387 file size 703937 SRR5423387 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423387 SRR5423387_1.fastq Input file: SRR5423387_1.fastq trimmed: SRR5423387-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Wed Feb 12 09:38:28 2025 >> started Wed Feb 12 09:38:30 2025 >> done (1.895s) 4000000 reads processed; of these: 93 ( 0.00%) short reads filtered out after trimming by size control 860 ( 0.02%) empty reads filtered out after trimming by size control 3999047 (99.98%) reads available; of these: 65273 ( 1.63%) trimmed reads available after processing 3933774 (98.37%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 9 0.00% 19 7 0.00% 20 9 0.00% 21 1 0.00% 22 1 0.00% 23 3 0.00% 24 2 0.00% 25 6 0.00% 26 7 0.00% 27 4 0.00% 28 7 0.00% 29 6 0.00% 30 7 0.00% 31 9 0.00% 32 13 0.00% 33 14 0.00% 34 16 0.00% 35 25 0.00% 36 35 0.00% 37 30 0.00% 38 45 0.00% 39 62 0.00% 40 66 0.00% 41 91 0.00% 42 116 0.00% 43 174 0.00% 44 236 0.01% 45 353 0.01% 46 511 0.01% 47 744 0.02% 48 1279 0.03% 49 2827 0.07% 50 7852 0.20% 51 50706 1.27% 52 3933774 98.37% 3999047 reads passed initial QC criterion=sequence-density sequence-density=0.38 sequence-density-rank=1 fanout-score=1.91 fanout-score-rank=27 prefix-density=0.36 prefix-fanout=1.9 sequence=TGGCACCGGCCCGTAATCCTTCCACTGCCAGGAGCGGGAGGTGATCGCTGCTCTGTGAGACCAGCCTCACGCTGTGCCCTCCCCTCTAGGAGCCACGACACC criterion=fanout-score sequence-density=0.04 sequence-density-rank=21 fanout-score=12.88 fanout-score-rank=1 prefix-density=0.21 prefix-fanout=2.6 sequence=GAGCTTCACCGTGTATGCTGCCATTGCTTTAACACGTCCTCCTCGACTGGCTTTCAAGCCAAGAAGAGACTCCCCCACGTTGGGAAGTGCCCGTAGGCTTGTCACTGGCACCCGGCGAGTAAACGATGTGCTGACCATGGCGCTGGAGAGGGCTGCTGTGGTGGCC Started job on | Feb 12 09:38:40 Started mapping on | Feb 12 09:38:40 Finished on | Feb 12 09:38:45 Mapping speed, Million of reads per hour | 2879.31 Number of input reads | 3999047 Average input read length | 51 UNIQUE READS: Uniquely mapped reads number | 3005353 Uniquely mapped reads % | 75.15% Average mapped length | 51.80 Number of splices: Total | 258447 Number of splices: Annotated (sjdb) | 255107 Number of splices: GT/AG | 250526 Number of splices: GC/AG | 6458 Number of splices: AT/AC | 831 Number of splices: Non-canonical | 632 Mismatch rate per base, % | 0.36% Deletion rate per base | 0.01% Deletion average length | 2.34 Insertion rate per base | 0.00% Insertion average length | 1.33 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 841694 % of reads mapped to multiple loci | 21.05% Number of reads mapped to too many loci | 75164 % of reads mapped to too many loci | 1.88% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.81% % of reads unmapped: other | 0.11% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 152000 152000 152000 N_multimapping 841694 841694 841694 N_noFeature 456061 2934789 515816 N_ambiguous 21925 148 10982 UnstrandedReadsAssigned:2527367 PositiveStrandReadsAssigned:70416 NegativeStrandReadsAssigned:2478555 Dataset is classified negative stranded MeadianReadLen=52 20thPercentileLength=52 echo kmer=47 SRR5423387 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR5423387-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,999,047 reads, 3,141,500 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,074 rounds 52401 SRR5423387.ke.tsv 34699 SRR5423387.se.tsv 87100 total ==> SRR5423387.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 88 12.2469 Potri.005G024800.1.v4.1 1035 936 6 1.71196 Potri.004G059700.1.v4.1 961 862 1 0.309821 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 69 6.47944 Potri.016G087400.1.v4.1 270 171 19 29.674 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 0 0 Potri.012G127500.1.v4.1 977 878 3 0.912525 ==> SRR5423387.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 5 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 21 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 3 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR5423387 completed mapping pipeline successfully