Starting /dee2/code/volunteer_pipeline.sh SRR5423391 current disk space = 3051614597120 free memory = 1560330504 SRR5423391 SRAfilesize 00b0f43383d5e5d11ca886969f2db122 SRR5423391.sra SRR5423391.sra file validated SRR5423391 is single end SRR5423391 is conventional basespace SRR5423391 read1 length is 52 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5423391_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.638 31.0 31.0 34.0 30.0 34.0 2 31.7825 33.0 31.0 34.0 30.0 34.0 3 31.987 34.0 31.0 34.0 30.0 34.0 4 33.8215 37.0 35.0 37.0 27.0 37.0 5 35.1075 37.0 35.0 37.0 32.0 37.0 6 35.25575 37.0 35.0 37.0 32.0 37.0 7 35.55225 37.0 35.0 37.0 33.0 37.0 8 35.543 37.0 35.0 37.0 33.0 37.0 9 37.21075 39.0 37.0 39.0 34.0 39.0 10 36.96975 39.0 37.0 39.0 33.0 39.0 11 37.23325 39.0 37.0 39.0 33.0 39.0 12 37.15525 39.0 37.0 39.0 33.0 39.0 13 36.9865 39.0 37.0 39.0 33.0 39.0 14 38.224 40.0 37.0 41.0 33.0 41.0 15 38.41025 40.0 38.0 41.0 33.0 41.0 16 38.1305 40.0 37.0 41.0 33.0 41.0 17 38.17975 40.0 37.0 41.0 33.0 41.0 18 38.20925 40.0 37.0 41.0 33.0 41.0 19 38.1245 40.0 37.0 41.0 33.0 41.0 20 38.1625 40.0 37.0 41.0 33.0 41.0 21 38.27625 40.0 38.0 41.0 33.0 41.0 22 38.42325 40.0 38.0 41.0 34.0 41.0 23 38.23575 40.0 38.0 41.0 33.0 41.0 24 38.13925 40.0 37.0 41.0 33.0 41.0 25 38.05475 40.0 37.0 41.0 33.0 41.0 26 37.83625 40.0 37.0 41.0 33.0 41.0 27 37.833 40.0 37.0 41.0 32.0 41.0 28 37.70825 40.0 37.0 41.0 32.0 41.0 29 37.76675 40.0 37.0 41.0 32.0 41.0 30 37.46975 40.0 37.0 41.0 32.0 41.0 31 37.532 40.0 37.0 41.0 31.0 41.0 32 37.505 40.0 37.0 41.0 32.0 41.0 33 37.6515 40.0 37.0 41.0 32.0 41.0 34 37.76275 40.0 37.0 41.0 33.0 41.0 35 37.57425 40.0 37.0 41.0 31.0 41.0 36 37.68 40.0 37.0 41.0 32.0 41.0 37 37.492 40.0 37.0 41.0 31.0 41.0 38 37.351 40.0 36.0 41.0 31.0 41.0 39 37.335 40.0 37.0 41.0 31.0 41.0 40 37.1275 39.0 36.0 41.0 30.0 41.0 41 37.3805 39.0 36.0 41.0 31.0 41.0 42 37.4935 39.0 37.0 41.0 32.0 41.0 43 37.314 39.0 36.0 41.0 31.0 41.0 44 36.93325 39.0 36.0 41.0 30.0 41.0 45 36.9755 39.0 36.0 41.0 30.0 41.0 46 36.926 39.0 35.0 40.0 31.0 41.0 47 36.91 39.0 35.0 41.0 30.0 41.0 48 36.82675 39.0 35.0 40.0 30.0 41.0 49 36.78275 39.0 35.0 40.0 30.0 41.0 50 36.86175 39.0 35.0 40.0 30.0 41.0 51 36.52225 39.0 35.0 40.0 30.0 41.0 52 35.3705 38.0 33.0 40.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1212 1 0.0 1212 2 0.0 1212 3 0.0 1212 4 0.0 1212 5 0.0 1212 6 0.0 1212 7 0.0 1212 8 0.0 1212 9 0.0 1212 10 0.0 1212 11 0.0 1212 12 0.0 1212 13 0.0 1212 14 0.0 1212 15 0.0 1212 16 0.0 1212 17 0.0 1212 18 0.0 1212 19 0.0 1212 20 0.0 1212 21 0.0 1212 22 0.0 1212 23 0.0 1212 24 0.0 1212 25 0.0 1212 26 0.0 1212 27 0.0 1212 28 0.0 1212 29 0.0 1212 30 0.0 1212 31 0.0 1212 32 0.0 1212 33 0.0 1212 34 0.0 1212 35 0.0 1212 36 0.0 1212 37 0.0 1212 38 0.0 1212 39 0.0 1212 40 0.0 1212 41 0.0 1212 42 0.0 1212 43 0.0 1212 44 0.0 1212 45 0.0 1212 46 0.0 1212 47 0.0 1212 48 0.0 1212 49 0.0 1212 50 0.0 1212 51 0.0 1212 52 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 4.0 22 3.0 23 8.0 24 7.0 25 15.0 26 17.0 27 22.0 28 38.0 29 55.0 30 78.0 31 102.0 32 140.0 33 159.0 34 195.0 35 268.0 36 349.0 37 472.0 38 767.0 39 1293.0 40 7.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.580742226680044 13.716148445336007 6.293881644934804 36.40922768304915 2 21.525 15.25 33.7 29.525000000000002 3 19.650000000000002 20.25 26.075 34.025 4 24.25 27.325 22.45 25.974999999999998 5 24.725 32.95 21.45 20.875 6 20.0 35.25 23.375 21.375 7 14.625 24.4 41.475 19.5 8 17.825 22.7 29.425 30.049999999999997 9 19.125 21.975 32.775 26.125 10 20.375 37.574999999999996 23.150000000000002 18.9 11 23.375 27.55 21.2 27.875 12 20.974999999999998 23.799999999999997 26.325 28.9 13 20.4 28.175 27.55 23.875 14 21.075 28.475 26.150000000000002 24.3 15 21.15 27.400000000000002 24.325 27.125 16 21.5 27.075 24.975 26.450000000000003 17 20.825 27.400000000000002 26.525 25.25 18 20.5 27.775 25.174999999999997 26.55 19 22.175 26.400000000000002 27.35 24.075 20 20.75 26.924999999999997 27.1 25.224999999999998 21 20.75 26.275 26.275 26.700000000000003 22 20.625 29.075 24.975 25.324999999999996 23 21.9 27.800000000000004 24.4 25.900000000000002 24 22.85 25.575 25.074999999999996 26.5 25 21.4 28.875 25.35 24.375 26 22.35 27.35 25.5 24.8 27 20.625 27.500000000000004 25.55 26.325 28 21.725 28.575 26.275 23.425 29 21.925 26.3 27.525 24.25 30 22.025 26.05 25.775 26.150000000000002 31 20.549999999999997 28.975 25.7 24.775 32 20.875 28.625 24.45 26.05 33 21.55 27.250000000000004 26.974999999999998 24.224999999999998 34 21.25 27.250000000000004 26.1 25.4 35 20.825 27.3 24.6 27.275 36 21.825 27.650000000000002 23.45 27.075 37 21.4 27.0 24.7 26.900000000000002 38 21.65 27.200000000000003 25.275 25.874999999999996 39 21.975 24.925 25.624999999999996 27.474999999999998 40 21.525 28.599999999999998 24.7 25.174999999999997 41 21.25 27.35 25.25 26.150000000000002 42 20.875 26.125 25.825 27.175 43 20.8 27.500000000000004 26.525 25.174999999999997 44 22.45 27.525 26.1 23.925 45 22.525000000000002 27.025 25.775 24.675 46 23.1 27.200000000000003 25.525 24.175 47 22.175 28.849999999999998 24.85 24.125 48 22.6 25.85 25.4 26.150000000000002 49 22.400000000000002 27.05 23.7 26.85 50 22.875 27.425 23.925 25.775 51 22.225 26.8 23.225 27.750000000000004 52 20.75 28.449999999999996 25.25 25.55 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5.0 1 2.5 2 0.0 3 1.5 4 3.0 5 1.5 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 1.0 12 2.0 13 1.0 14 1.5 15 3.0 16 3.5 17 4.0 18 4.0 19 4.0 20 7.0 21 10.0 22 13.5 23 17.0 24 19.5 25 22.0 26 25.5 27 29.0 28 33.0 29 37.0 30 45.0 31 53.0 32 64.5 33 76.0 34 111.0 35 146.0 36 160.5 37 175.0 38 195.5 39 232.5 40 249.0 41 243.0 42 237.0 43 263.5 44 290.0 45 279.0 46 268.0 47 269.0 48 270.0 49 287.5 50 305.0 51 311.5 52 318.0 53 320.5 54 323.0 55 284.0 56 245.0 57 226.5 58 208.0 59 193.0 60 178.0 61 156.5 62 135.0 63 101.5 64 55.5 65 43.0 66 36.5 67 30.0 68 19.5 69 9.0 70 8.0 71 7.0 72 6.5 73 6.0 74 4.5 75 3.0 76 3.0 77 3.0 78 2.0 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.3 #Duplication Level Percentage of deduplicated Percentage of total 1 93.20498301245753 82.3 2 4.048697621744054 7.1499999999999995 3 1.274065685164213 3.375 4 0.594563986409966 2.1 5 0.3114382785956965 1.375 6 0.16987542468856173 0.8999999999999999 7 0.19818799546998866 1.225 8 0.11325028312570783 0.8 9 0.028312570781426957 0.22499999999999998 >10 0.05662514156285391 0.5499999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGA 11 0.27499999999999997 No Hit CGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGC 11 0.27499999999999997 No Hit GCCAGACTAAGCAATAAAGTACCTCGTATTTTCCCCTCTGCCTCGGGTTGTC 9 0.22499999999999998 No Hit GCCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTAC 8 0.2 No Hit CTCACGAGCAAGATCACGTCCCTCATTACGAGCTTGTACACATGCTTCTAGA 8 0.2 No Hit CCCTGATCAAACTAGAGGTTACCAAAGAACCATGCATAGCACTGAATAGGGA 8 0.2 No Hit CAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACTGGT 8 0.2 No Hit GGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGT 7 0.17500000000000002 No Hit CAGGTATTAATTACACGACCTTGACTATCAACTACAGATTGGTTGAAATTGA 7 0.17500000000000002 No Hit CCCAGGTATTAATTACACGACCTTGACTATCAACTACAGATTGGTTGAAATT 7 0.17500000000000002 No Hit GTCCCTTATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCA 7 0.17500000000000002 No Hit CCAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACTGG 7 0.17500000000000002 No Hit GCCGAATACACCAGCTACGCCTAACATGTGAAATGGGTGCATAAGGATGTTG 7 0.17500000000000002 No Hit GCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGATCA 7 0.17500000000000002 No Hit ATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCAACTACAGATT 6 0.15 No Hit CCAGGTATTAATTACACGACCTTGACTATCAACTACAGATTGGTTGAAATTG 6 0.15 No Hit GCACGGTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCAA 6 0.15 No Hit GTTCAATTAGATCAGCCCATCAGGCCATGCGGATCCAGGTGGCACCGGCCCG 6 0.15 No Hit GTCGGTCCATGGATTTTCCTTCCTTTTTCCGCATTTCGCTAAAGGGTTGAAG 6 0.15 No Hit GCCCCATCTATCCTCCTGAGGAGAAGTTTGGTTTCAAACCCCGGTTCGAACA 6 0.15 No Hit GTCCCTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAAC 5 0.125 No Hit CTCGTATTTTCCCCTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTG 5 0.125 No Hit GCTCAGCCTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGAGG 5 0.125 No Hit GCAGCAATGAAAGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGAA 5 0.125 No Hit GTTCAATTAGGACCAGCCCATCAGACCATGCGGATCCAGATGGCACCGACCC 5 0.125 No Hit GTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCAACTACA 5 0.125 No Hit CCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACT 5 0.125 No Hit CCGTAATCCTTCCACTGCCAGGAGCGGGAGGTGATCGCTGCTCTGTGAGACC 5 0.125 No Hit GTGACCGGGAAAGCTGAGGTAGACTTGAGGCCGTTGAATGGTGCAACCATGT 5 0.125 No Hit GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGTA 5 0.125 No Hit AGCACGGTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.025 0.0 0.0 0.0 0.0 33 0.025 0.0 0.0 0.0 0.0 34 0.025 0.0 0.0 0.0 0.0 35 0.025 0.0 0.0 0.0 0.0 36 0.025 0.0 0.0 0.0 0.0 37 0.025 0.0 0.0 0.0 0.0 38 0.025 0.0 0.0 0.0 0.0 39 0.025 0.0 0.0 0.0 0.0 40 0.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra Read 200000 spots for SRR5423391.sra Written 200000 spots for SRR5423391.sra SRR ids: ['SRR5423391.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_57qpp2x5 SRR5423391.sra spots: 4000000 blocks: [[1, 200000], [200001, 400000], [400001, 600000], [600001, 800000], [800001, 1000000], [1000001, 1200000], [1200001, 1400000], [1400001, 1600000], [1600001, 1800000], [1800001, 2000000], [2000001, 2200000], [2200001, 2400000], [2400001, 2600000], [2600001, 2800000], [2800001, 3000000], [3000001, 3200000], [3200001, 3400000], [3400001, 3600000], [3600001, 3800000], [3800001, 4000000]] SRR5423391 file size 703970 SRR5423391 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423391 SRR5423391_1.fastq Input file: SRR5423391_1.fastq trimmed: SRR5423391-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Thu Feb 13 05:09:19 2025 >> started Thu Feb 13 05:10:46 2025 >> done (87.293s) 4000000 reads processed; of these: 84 ( 0.00%) short reads filtered out after trimming by size control 912 ( 0.02%) empty reads filtered out after trimming by size control 3999004 (99.98%) reads available; of these: 95414 ( 2.39%) trimmed reads available after processing 3903590 (97.61%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 8 0.00% 19 3 0.00% 20 9 0.00% 21 1 0.00% 22 2 0.00% 23 3 0.00% 24 5 0.00% 25 3 0.00% 26 6 0.00% 27 10 0.00% 28 7 0.00% 29 8 0.00% 30 11 0.00% 31 11 0.00% 32 12 0.00% 33 12 0.00% 34 24 0.00% 35 28 0.00% 36 44 0.00% 37 44 0.00% 38 46 0.00% 39 65 0.00% 40 81 0.00% 41 104 0.00% 42 141 0.00% 43 193 0.00% 44 298 0.01% 45 453 0.01% 46 639 0.02% 47 999 0.02% 48 1850 0.05% 49 3974 0.10% 50 11552 0.29% 51 74768 1.87% 52 3903590 97.61% 3999004 reads passed initial QC criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=1.91 fanout-score-rank=26 prefix-density=0.34 prefix-fanout=1.9 sequence=TGGCACCGGCCCGTAATCCTTCCACTGCCAGGAGCGGGAGGTGATCGCTGCTCTGTGAGACCAGCCTCACGCTGTGCCCTCCCCTCTAGGAGCCACGACACC criterion=fanout-score sequence-density=0.04 sequence-density-rank=20 fanout-score=12.94 fanout-score-rank=1 prefix-density=0.20 prefix-fanout=2.5 sequence=GAGCTTCACCGTGTATGCTGCCATTGCTTTAACACGTCCTCCTCGACTGGCTTTCAAGCCAAGAAGAGACTCCCCCACGTTGGGAAGTGCCCGTAGGCTTGTCACTGGCACCCGGCGAGTAAACGATGTGCTGACCATGGCGCTGGAGAGGGCTGCTGTGGTGGC Started job on | Feb 13 05:13:29 Started mapping on | Feb 13 05:13:40 Finished on | Feb 13 05:29:15 Mapping speed, Million of reads per hour | 15.40 Number of input reads | 3999004 Average input read length | 51 UNIQUE READS: Uniquely mapped reads number | 3002297 Uniquely mapped reads % | 75.08% Average mapped length | 51.79 Number of splices: Total | 257831 Number of splices: Annotated (sjdb) | 254430 Number of splices: GT/AG | 249913 Number of splices: GC/AG | 6341 Number of splices: AT/AC | 878 Number of splices: Non-canonical | 699 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.01% Deletion average length | 2.32 Insertion rate per base | 0.00% Insertion average length | 1.34 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 845572 % of reads mapped to multiple loci | 21.14% Number of reads mapped to too many loci | 74071 % of reads mapped to too many loci | 1.85% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.80% % of reads unmapped: other | 0.12% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 151135 151135 151135 N_multimapping 845572 845572 845572 N_noFeature 454427 2932239 513606 N_ambiguous 22253 159 11231 UnstrandedReadsAssigned:2525617 PositiveStrandReadsAssigned:69899 NegativeStrandReadsAssigned:2477460 Dataset is classified negative stranded MeadianReadLen=52 20thPercentileLength=52 echo kmer=47 SRR5423391 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR5423391-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,999,004 reads, 3,154,005 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,097 rounds 52401 SRR5423391.ke.tsv 34699 SRR5423391.se.tsv 87100 total ==> SRR5423391.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 93 12.9526 Potri.005G024800.1.v4.1 1035 936 3 0.856629 Potri.004G059700.1.v4.1 961 862 3 0.930168 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 69.5967 6.54044 Potri.016G087400.1.v4.1 270 171 11 17.1927 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 2 0.319317 Potri.012G127500.1.v4.1 977 878 2 0.608812 ==> SRR5423391.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 3 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 20 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 2 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 4 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1 SRR5423391 completed mapping pipeline successfully