Starting /dee2/code/volunteer_pipeline.sh SRR5423394 current disk space = 3051950891008 free memory = 1562142444 SRR5423394 SRAfilesize ca1acddc40466335748b69a654451869 SRR5423394.sra SRR5423394.sra file validated SRR5423394 is single end SRR5423394 is conventional basespace SRR5423394 read1 length is 52 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5423394_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.274 34.0 31.0 34.0 30.0 34.0 2 32.41675 34.0 31.0 34.0 30.0 34.0 3 32.57675 34.0 31.0 34.0 31.0 34.0 4 36.032 37.0 35.0 37.0 35.0 37.0 5 35.948 37.0 35.0 37.0 35.0 37.0 6 35.944 37.0 35.0 37.0 35.0 37.0 7 35.982 37.0 35.0 37.0 35.0 37.0 8 35.94525 37.0 35.0 37.0 35.0 37.0 9 37.7435 39.0 37.0 39.0 35.0 39.0 10 37.6215 39.0 37.0 39.0 35.0 39.0 11 37.7435 39.0 37.0 39.0 35.0 39.0 12 37.77625 39.0 38.0 39.0 35.0 39.0 13 37.66875 39.0 37.0 39.0 35.0 39.0 14 39.07425 40.0 39.0 41.0 36.0 41.0 15 39.01775 40.0 38.0 41.0 36.0 41.0 16 38.97775 40.0 38.0 41.0 36.0 41.0 17 39.006 40.0 38.0 41.0 36.0 41.0 18 39.017 40.0 38.0 41.0 36.0 41.0 19 39.01675 40.0 39.0 41.0 36.0 41.0 20 38.9605 40.0 38.0 41.0 35.0 41.0 21 38.831 40.0 38.0 41.0 35.0 41.0 22 38.849 40.0 38.0 41.0 35.0 41.0 23 38.79175 40.0 38.0 41.0 35.0 41.0 24 38.853 40.0 38.0 41.0 35.0 41.0 25 38.708 40.0 38.0 41.0 34.0 41.0 26 38.72 40.0 38.0 41.0 34.0 41.0 27 38.594 40.0 38.0 41.0 34.0 41.0 28 38.5915 40.0 38.0 41.0 34.0 41.0 29 38.3715 40.0 38.0 41.0 34.0 41.0 30 38.39725 40.0 38.0 41.0 34.0 41.0 31 38.37225 40.0 38.0 41.0 34.0 41.0 32 38.4105 40.0 38.0 41.0 34.0 41.0 33 38.3185 40.0 38.0 41.0 34.0 41.0 34 38.1835 40.0 38.0 41.0 33.0 41.0 35 38.0415 40.0 38.0 41.0 33.0 41.0 36 38.1335 40.0 38.0 41.0 33.0 41.0 37 38.03275 40.0 38.0 41.0 33.0 41.0 38 37.82825 40.0 38.0 41.0 33.0 41.0 39 37.89925 40.0 38.0 41.0 33.0 41.0 40 37.7845 40.0 37.0 41.0 33.0 41.0 41 37.877 40.0 37.0 41.0 33.0 41.0 42 37.88125 40.0 38.0 41.0 33.0 41.0 43 37.79 40.0 37.0 41.0 32.0 41.0 44 37.4425 40.0 37.0 41.0 31.0 41.0 45 37.4155 40.0 37.0 41.0 31.0 41.0 46 37.38475 40.0 37.0 41.0 31.0 41.0 47 37.274 40.0 37.0 41.0 31.0 41.0 48 37.1415 40.0 36.0 41.0 31.0 41.0 49 37.15775 40.0 36.0 41.0 31.0 41.0 50 37.00925 40.0 36.0 41.0 30.0 41.0 51 36.8295 39.0 36.0 41.0 30.0 41.0 52 35.21775 38.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2205 1 0.0 2205 2 0.0 2205 3 0.0 2205 4 0.0 2205 5 0.0 2205 6 0.0 2205 7 0.0 2205 8 0.0 2205 9 0.0 2205 10 0.0 2205 11 0.0 2205 12 0.0 2205 13 0.0 2205 14 0.0 2205 15 0.0 2205 16 0.0 2205 17 0.0 2205 18 0.0 2205 19 0.0 2205 20 0.0 2205 21 0.0 2205 22 0.0 2205 23 0.0 2205 24 0.0 2205 25 0.0 2205 26 0.0 2205 27 0.0 2205 28 0.0 2205 29 0.0 2205 30 0.0 2205 31 0.0 2205 32 0.0 2205 33 0.0 2205 34 0.0 2205 35 0.0 2205 36 0.0 2205 37 0.0 2205 38 0.0 2205 39 0.0 2205 40 0.0 2205 41 0.0 2205 42 0.0 2205 43 0.0 2205 44 0.0 2205 45 0.0 2205 46 0.0 2205 47 0.0 2205 48 0.0 2205 49 0.0 2205 50 0.0 2205 51 0.0 2205 52 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 0.0 21 0.0 22 3.0 23 7.0 24 10.0 25 5.0 26 19.0 27 15.0 28 28.0 29 48.0 30 52.0 31 62.0 32 87.0 33 106.0 34 129.0 35 224.0 36 286.0 37 431.0 38 774.0 39 1705.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.9861982434128 13.350062735257215 6.57465495608532 37.08908406524466 2 21.8 14.774999999999999 35.075 28.349999999999998 3 20.349999999999998 19.125 23.724999999999998 36.8 4 25.724999999999998 27.35 21.65 25.275 5 24.275 32.75 21.675 21.3 6 20.925 35.099999999999994 22.25 21.725 7 15.55 24.425 40.425 19.6 8 17.224999999999998 22.775000000000002 29.975 30.025000000000002 9 19.275000000000002 21.025 32.0 27.700000000000003 10 18.675 39.75 22.5 19.075 11 24.9 28.925 20.8 25.374999999999996 12 21.925 25.25 24.65 28.175 13 20.45 28.15 27.650000000000002 23.75 14 21.224999999999998 28.799999999999997 26.200000000000003 23.775 15 21.475 26.875 26.400000000000002 25.25 16 21.775 27.200000000000003 26.5 24.525 17 23.175 26.900000000000002 25.4 24.525 18 21.875 26.525 25.424999999999997 26.174999999999997 19 22.825 27.05 26.0 24.125 20 21.725 25.900000000000002 26.55 25.825 21 21.15 27.925 25.45 25.474999999999998 22 20.724999999999998 27.375 25.55 26.35 23 21.425 29.5 24.425 24.65 24 21.125 27.075 25.6 26.200000000000003 25 22.2 26.825 24.474999999999998 26.5 26 21.5 26.924999999999997 25.974999999999998 25.6 27 21.4 26.55 26.525 25.525 28 22.400000000000002 28.975 24.95 23.674999999999997 29 21.325 28.15 26.224999999999998 24.3 30 21.6 25.85 25.8 26.75 31 21.75 28.549999999999997 25.025 24.675 32 21.95 26.875 25.4 25.775 33 20.75 24.975 26.6 27.675 34 22.075 27.725 25.474999999999998 24.725 35 21.125 27.525 24.825 26.525 36 21.0 27.900000000000002 23.3 27.800000000000004 37 21.275 26.6 26.75 25.374999999999996 38 21.8 27.325 25.35 25.525 39 21.15 25.4 25.374999999999996 28.075 40 21.55 28.125 24.625 25.7 41 22.025 26.375 25.575 26.025 42 21.5 25.0 25.8 27.700000000000003 43 22.05 28.000000000000004 24.5 25.45 44 22.675 27.125 25.650000000000002 24.55 45 22.73068267066767 25.95648912228057 24.956239059764943 26.356589147286826 46 23.755938984746187 26.6816704176044 24.756189047261813 24.8062015503876 47 24.18104526131533 27.031757939484873 23.830957739434858 24.956239059764943 48 22.355588897224308 26.481620405101275 24.63115778944736 26.531632908227053 49 22.3 26.700000000000003 24.975 26.025 50 23.275000000000002 28.125 23.925 24.675 51 22.13053263315829 26.431607901975497 24.681170292573142 26.756689172293076 52 22.175 27.3 24.625 25.900000000000002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 2.0 2 2.0 3 2.0 4 2.0 5 1.5 6 1.0 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.5 15 2.0 16 2.5 17 3.0 18 3.0 19 3.0 20 8.0 21 13.0 22 13.0 23 13.0 24 14.0 25 15.0 26 19.0 27 23.0 28 27.5 29 32.0 30 44.5 31 57.0 32 69.5 33 82.0 34 100.0 35 118.0 36 138.0 37 158.0 38 191.5 39 220.5 40 216.0 41 221.5 42 227.0 43 263.0 44 299.0 45 303.5 46 308.0 47 310.5 48 313.0 49 309.0 50 305.0 51 323.5 52 342.0 53 333.0 54 324.0 55 280.5 56 237.0 57 213.0 58 189.0 59 178.5 60 168.0 61 140.0 62 112.0 63 91.5 64 60.0 65 49.0 66 42.5 67 36.0 68 29.0 69 22.0 70 17.0 71 12.0 72 8.5 73 5.0 74 4.5 75 4.0 76 4.5 77 5.0 78 3.5 79 2.0 80 1.5 81 1.0 82 1.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.025 46 0.025 47 0.025 48 0.025 49 0.0 50 0.0 51 0.025 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.375 #Duplication Level Percentage of deduplicated Percentage of total 1 92.87128712871288 82.075 2 4.582743988684583 8.1 3 1.3295615275813295 3.5249999999999995 4 0.4243281471004243 1.5 5 0.2545968882602546 1.125 6 0.2545968882602546 1.35 7 0.08486562942008487 0.525 8 0.056577086280056574 0.4 9 0.028288543140028287 0.22499999999999998 >10 0.11315417256011315 1.175 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACTGG 14 0.35000000000000003 No Hit GCCAGACTAAGCAATAAAGTACCTCGTATTTTCCCCTCTGCCTCGGGTTGTC 12 0.3 No Hit CGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGC 11 0.27499999999999997 No Hit GCCTAACATGTGAAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACA 10 0.25 No Hit CCCTGATCAAACTAGAGGTTACCAAAGAACCATGCATAGCACTGAATAGGGA 9 0.22499999999999998 No Hit CCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACT 8 0.2 No Hit GGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGA 8 0.2 No Hit CTCGTATTTTCCCCTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTG 7 0.17500000000000002 No Hit GTCCCTTATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCA 7 0.17500000000000002 No Hit GTTTCCATAAAGTAGAGATCCAGAAACAGGTTCACGAATACCATCAATGTCT 7 0.17500000000000002 No Hit AATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCAACTACAGAT 6 0.15 No Hit GTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCG 6 0.15 No Hit CACGGTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCAAC 6 0.15 No Hit GCACGGTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCAA 6 0.15 No Hit GTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCC 6 0.15 No Hit CTAACATGTGAAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAAT 6 0.15 No Hit GCCGAATACACCAGCTACGCCTAACATGTGAAATGGGTGCATAAGGATGTTG 6 0.15 No Hit GCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGATCA 6 0.15 No Hit AGCACGGTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCA 6 0.15 No Hit GGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCC 5 0.125 No Hit CTCACGAGCAAGATCACGTCCCTCATTACGAGCTTGTACACATGCTTCTAGA 5 0.125 No Hit GTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCAACTACA 5 0.125 No Hit CCCAGGTATTAATTACACGACCTTGACTATCAACTACAGATTGGTTGAAATT 5 0.125 No Hit GTAAGCTTTCTTCGCCTCCTCGAGCTCAATCAGCACCTGAGATGCCTCAGTG 5 0.125 No Hit CTGGTAAGCCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAG 5 0.125 No Hit GGGGTGGAGACGATGGGGTCGGTCCATGGATTTTCCTTCCTTTTTCCGCATT 5 0.125 No Hit GGGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCG 5 0.125 No Hit CTCGATTAGCAACGGCACCGGGTGCATTTCCCCAAGGGTGCCCTAAAGTTCC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra Read 200000 spots for SRR5423394.sra Written 200000 spots for SRR5423394.sra SRR ids: ['SRR5423394.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_jb9i_tit SRR5423394.sra spots: 4000000 blocks: [[1, 200000], [200001, 400000], [400001, 600000], [600001, 800000], [800001, 1000000], [1000001, 1200000], [1200001, 1400000], [1400001, 1600000], [1600001, 1800000], [1800001, 2000000], [2000001, 2200000], [2200001, 2400000], [2400001, 2600000], [2600001, 2800000], [2800001, 3000000], [3000001, 3200000], [3200001, 3400000], [3400001, 3600000], [3600001, 3800000], [3800001, 4000000]] SRR5423394 file size 703951 SRR5423394 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423394 SRR5423394_1.fastq Input file: SRR5423394_1.fastq trimmed: SRR5423394-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Thu Feb 13 05:54:37 2025 >> started Thu Feb 13 05:55:14 2025 >> done (36.524s) 4000000 reads processed; of these: 85 ( 0.00%) short reads filtered out after trimming by size control 898 ( 0.02%) empty reads filtered out after trimming by size control 3999017 (99.98%) reads available; of these: 94230 ( 2.36%) trimmed reads available after processing 3904787 (97.64%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 14 0.00% 19 1 0.00% 20 7 0.00% 21 2 0.00% 22 3 0.00% 23 6 0.00% 24 5 0.00% 25 6 0.00% 26 5 0.00% 27 4 0.00% 28 9 0.00% 29 8 0.00% 30 5 0.00% 31 18 0.00% 32 16 0.00% 33 7 0.00% 34 23 0.00% 35 24 0.00% 36 27 0.00% 37 29 0.00% 38 35 0.00% 39 59 0.00% 40 93 0.00% 41 114 0.00% 42 156 0.00% 43 196 0.00% 44 349 0.01% 45 430 0.01% 46 604 0.02% 47 1019 0.03% 48 1739 0.04% 49 3723 0.09% 50 11208 0.28% 51 74286 1.86% 52 3904787 97.64% 3999017 reads passed initial QC criterion=sequence-density sequence-density=0.37 sequence-density-rank=1 fanout-score=1.90 fanout-score-rank=25 prefix-density=0.35 prefix-fanout=1.9 sequence=TGGCACCGGCCCGTAATCCTTCCACTGCCAGGAGCGGGAGGTGATCGCTGCTCTGTGAGACCAGCCTCACGCTGTGCCCTCCCCTCTAGGAGCCACGACACC criterion=fanout-score sequence-density=0.04 sequence-density-rank=20 fanout-score=12.95 fanout-score-rank=1 prefix-density=0.21 prefix-fanout=2.5 sequence=GAGCTTCACCGTGTATGCTGCCATTGCTTTAACACGTCCTCCTCGACTGGCTTTCAAGCCAAGAAGAGACTCCCCCACGTTGGGAAGTGCCCGTAGGCTTGTCACTGGCACCCGGCGAGTAAACGATGTGCTGACCATGGCGCTGGAGAGGGCTGCTGTGGTGGCCA Started job on | Feb 13 05:57:20 Started mapping on | Feb 13 05:57:31 Finished on | Feb 13 05:58:35 Mapping speed, Million of reads per hour | 224.94 Number of input reads | 3999017 Average input read length | 51 UNIQUE READS: Uniquely mapped reads number | 3000470 Uniquely mapped reads % | 75.03% Average mapped length | 51.79 Number of splices: Total | 257087 Number of splices: Annotated (sjdb) | 253780 Number of splices: GT/AG | 249297 Number of splices: GC/AG | 6302 Number of splices: AT/AC | 825 Number of splices: Non-canonical | 663 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.01% Deletion average length | 2.31 Insertion rate per base | 0.00% Insertion average length | 1.34 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 847427 % of reads mapped to multiple loci | 21.19% Number of reads mapped to too many loci | 73480 % of reads mapped to too many loci | 1.84% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.81% % of reads unmapped: other | 0.13% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 151120 151120 151120 N_multimapping 847427 847427 847427 N_noFeature 455244 2930182 514553 N_ambiguous 22368 164 11234 UnstrandedReadsAssigned:2522858 PositiveStrandReadsAssigned:70124 NegativeStrandReadsAssigned:2474683 Dataset is classified negative stranded MeadianReadLen=52 20thPercentileLength=52 echo kmer=47 SRR5423394 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR5423394-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,999,017 reads, 3,145,985 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,052 rounds 52401 SRR5423394.ke.tsv 34699 SRR5423394.se.tsv 87100 total ==> SRR5423394.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 91 12.6922 Potri.005G024800.1.v4.1 1035 936 0 0 Potri.004G059700.1.v4.1 961 862 4 1.242 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 67.6988 6.37121 Potri.016G087400.1.v4.1 270 171 22 34.4348 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 0 0 Potri.012G127500.1.v4.1 977 878 2 0.609686 ==> SRR5423394.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 5 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 33 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 10 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR5423394 completed mapping pipeline successfully