Starting /dee2/code/volunteer_pipeline.sh SRR5423395 current disk space = 3051951452160 free memory = 1560561332 SRR5423395 SRAfilesize 892e1ce27094cfb92d09e2faa4d86139 SRR5423395.sra SRR5423395.sra file validated SRR5423395 is single end SRR5423395 is conventional basespace SRR5423395 read1 length is 52 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5423395_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.452 34.0 31.0 34.0 30.0 34.0 2 32.44125 34.0 31.0 34.0 30.0 34.0 3 32.56075 34.0 31.0 34.0 31.0 34.0 4 35.965 37.0 35.0 37.0 35.0 37.0 5 35.84175 37.0 35.0 37.0 35.0 37.0 6 35.8675 37.0 35.0 37.0 35.0 37.0 7 35.98175 37.0 35.0 37.0 35.0 37.0 8 35.98975 37.0 35.0 37.0 35.0 37.0 9 37.704 39.0 38.0 39.0 35.0 39.0 10 37.4895 39.0 37.0 39.0 35.0 39.0 11 37.64725 39.0 37.0 39.0 35.0 39.0 12 37.6155 39.0 37.0 39.0 35.0 39.0 13 37.6105 39.0 37.0 39.0 35.0 39.0 14 38.99425 40.0 38.0 41.0 36.0 41.0 15 38.9475 40.0 38.0 41.0 35.0 41.0 16 38.9545 40.0 38.0 41.0 36.0 41.0 17 38.97575 40.0 38.0 41.0 36.0 41.0 18 38.9585 40.0 38.0 41.0 36.0 41.0 19 38.81875 40.0 38.0 41.0 35.0 41.0 20 38.9075 40.0 38.0 41.0 35.0 41.0 21 38.9355 40.0 38.0 41.0 35.0 41.0 22 38.9785 40.0 39.0 41.0 35.0 41.0 23 38.72925 40.0 38.0 41.0 34.0 41.0 24 38.53325 40.0 38.0 41.0 34.0 41.0 25 38.40575 40.0 38.0 41.0 34.0 41.0 26 38.27325 40.0 38.0 41.0 34.0 41.0 27 38.30425 40.0 38.0 41.0 34.0 41.0 28 38.41675 40.0 38.0 41.0 34.0 41.0 29 38.097 40.0 38.0 41.0 33.0 41.0 30 37.9925 40.0 38.0 41.0 33.0 41.0 31 38.17975 40.0 38.0 41.0 33.0 41.0 32 38.05275 40.0 38.0 41.0 33.0 41.0 33 37.98175 40.0 38.0 41.0 33.0 41.0 34 37.94125 40.0 38.0 41.0 33.0 41.0 35 37.9165 40.0 38.0 41.0 33.0 41.0 36 38.01725 40.0 38.0 41.0 33.0 41.0 37 37.852 40.0 38.0 41.0 33.0 41.0 38 37.518 40.0 37.0 41.0 32.0 41.0 39 37.35925 40.0 37.0 41.0 31.0 41.0 40 37.1475 40.0 37.0 41.0 30.0 41.0 41 37.358 40.0 37.0 41.0 31.0 41.0 42 37.2565 40.0 37.0 41.0 31.0 41.0 43 36.95275 40.0 36.0 41.0 30.0 41.0 44 37.31 40.0 37.0 41.0 31.0 41.0 45 37.07825 40.0 37.0 41.0 31.0 41.0 46 36.99425 40.0 36.0 41.0 30.0 41.0 47 36.936 40.0 36.0 41.0 31.0 41.0 48 36.701 39.0 36.0 41.0 30.0 41.0 49 36.504 39.0 35.0 41.0 29.0 41.0 50 36.5105 39.0 35.0 41.0 29.0 41.0 51 36.40225 39.0 35.0 41.0 29.0 41.0 52 34.3715 38.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2301 1 0.0 2301 2 0.0 2301 3 0.0 2301 4 0.0 2301 5 0.0 2301 6 0.0 2301 7 0.0 2301 8 0.0 2301 9 0.0 2301 10 0.0 2301 11 0.0 2301 12 0.0 2301 13 0.0 2301 14 0.0 2301 15 0.0 2301 16 0.0 2301 17 0.0 2301 18 0.0 2301 19 0.0 2301 20 0.0 2301 21 0.0 2301 22 0.0 2301 23 0.0 2301 24 0.0 2301 25 0.0 2301 26 0.0 2301 27 0.0 2301 28 0.0 2301 29 0.0 2301 30 0.0 2301 31 0.0 2301 32 0.0 2301 33 0.0 2301 34 0.0 2301 35 0.0 2301 36 0.0 2301 37 0.0 2301 38 0.0 2301 39 0.0 2301 40 0.0 2301 41 0.0 2301 42 0.0 2301 43 0.0 2301 44 0.0 2301 45 0.0 2301 46 0.0 2301 47 0.0 2301 48 0.0 2301 49 0.0 2301 50 0.0 2301 51 0.0 2301 52 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 0.0 12 1.0 13 0.0 14 1.0 15 0.0 16 0.0 17 0.0 18 0.0 19 2.0 20 1.0 21 3.0 22 4.0 23 9.0 24 12.0 25 19.0 26 24.0 27 25.0 28 34.0 29 36.0 30 61.0 31 59.0 32 90.0 33 131.0 34 158.0 35 225.0 36 286.0 37 412.0 38 785.0 39 1618.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.66141535383846 12.85321330332583 5.87646911727932 35.608902225556385 2 21.725 14.075 36.425000000000004 27.775 3 19.475 19.075 24.65 36.8 4 24.625 27.425 20.95 27.0 5 23.9 33.074999999999996 23.575 19.45 6 20.025000000000002 35.199999999999996 22.525000000000002 22.25 7 16.5 25.224999999999998 39.574999999999996 18.7 8 18.75 23.1 30.225 27.925 9 18.0 22.125 32.475 27.400000000000002 10 19.3 37.95 23.875 18.875 11 24.65 28.375 19.625 27.35 12 20.8 25.95 25.724999999999998 27.525 13 19.375 28.9 28.4 23.325000000000003 14 22.125 27.85 25.8 24.224999999999998 15 20.8 27.0 26.75 25.45 16 20.325 26.8 26.424999999999997 26.450000000000003 17 22.900000000000002 25.775 26.575 24.75 18 20.325 27.85 26.974999999999998 24.85 19 21.4 27.625 25.275 25.7 20 20.875 26.674999999999997 26.625 25.825 21 21.425 26.424999999999997 25.924999999999997 26.224999999999998 22 21.15 28.525 25.674999999999997 24.65 23 21.475 28.225 25.025 25.275 24 22.575 26.674999999999997 25.55 25.2 25 22.825 27.875 24.65 24.65 26 21.45 27.575 24.7 26.275 27 21.25 27.625 25.924999999999997 25.2 28 21.275 27.55 27.3 23.875 29 21.725 26.55 26.55 25.174999999999997 30 20.275000000000002 25.224999999999998 25.724999999999998 28.775000000000002 31 20.549999999999997 29.125 25.3 25.025 32 22.0 26.924999999999997 25.525 25.55 33 20.7 25.45 27.950000000000003 25.900000000000002 34 20.674999999999997 27.650000000000002 26.3 25.374999999999996 35 20.75 27.35 24.45 27.450000000000003 36 21.8 27.750000000000004 23.625 26.825 37 21.525 27.650000000000002 25.45 25.374999999999996 38 22.7 26.400000000000002 24.7 26.200000000000003 39 21.875 25.8 25.1 27.224999999999998 40 21.80545136284071 29.232308077019255 25.056264066016503 23.905976494123532 41 22.1 26.3 24.525 27.075 42 21.725 25.424999999999997 26.224999999999998 26.625 43 22.2 29.275000000000002 22.95 25.575 44 23.075000000000003 26.85 25.624999999999996 24.45 45 22.225 26.75 25.025 26.0 46 24.224999999999998 26.25 25.025 24.5 47 22.80570142535634 27.656914228557138 24.90622655663916 24.63115778944736 48 22.35 26.625 24.175 26.85 49 21.48037009252313 27.7569392348087 25.456364091022753 25.30632658164541 50 22.775000000000002 26.150000000000002 25.3 25.775 51 21.030257564391096 25.906476619154787 25.35633908477119 27.70692673168292 52 22.650000000000002 28.000000000000004 24.45 24.9 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 1.5 2 1.0 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 1.5 12 2.0 13 1.5 14 1.0 15 1.0 16 1.5 17 2.0 18 5.0 19 8.0 20 8.5 21 9.0 22 10.0 23 11.0 24 12.5 25 14.0 26 13.5 27 13.0 28 26.0 29 39.0 30 50.0 31 61.0 32 81.5 33 102.0 34 114.5 35 127.0 36 147.5 37 168.0 38 190.0 39 227.5 40 243.0 41 244.5 42 246.0 43 258.0 44 270.0 45 286.5 46 303.0 47 296.0 48 289.0 49 291.0 50 293.0 51 338.5 52 384.0 53 347.5 54 311.0 55 281.5 56 252.0 57 228.5 58 205.0 59 175.5 60 146.0 61 129.0 62 112.0 63 84.0 64 48.0 65 40.0 66 34.5 67 29.0 68 23.5 69 18.0 70 13.0 71 8.0 72 8.0 73 8.0 74 6.0 75 4.0 76 4.5 77 5.0 78 3.0 79 1.0 80 1.0 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.025 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.025 48 0.0 49 0.025 50 0.0 51 0.025 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.1 #Duplication Level Percentage of deduplicated Percentage of total 1 92.84903518728717 81.8 2 4.45516458569807 7.85 3 1.2769580022701474 3.375 4 0.6242905788876276 2.1999999999999997 5 0.22701475595913734 1.0 6 0.170261066969353 0.8999999999999999 7 0.170261066969353 1.05 8 0.11350737797956867 0.8 9 0.028376844494892167 0.22499999999999998 >10 0.0851305334846765 0.8 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCAGGTATTAATTACACGACCTTGACTATCAACTACAGATTGGTTGAAATT 12 0.3 No Hit CGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGC 10 0.25 No Hit GCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGATCA 10 0.25 No Hit GTCCCTTATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCA 9 0.22499999999999998 No Hit GTGCTGAGTTGGAATCCCATTCTAACTAAGGATTCTTGTGGTTCCGGAGGAT 8 0.2 No Hit GGTCGGTCCATGGATTTTCCTTCCTTTTTCCGCATTTCGCTAAAGGGTTGAA 8 0.2 No Hit CCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACT 8 0.2 No Hit GGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGA 8 0.2 No Hit GGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGT 7 0.17500000000000002 No Hit CAGGTATTAATTACACGACCTTGACTATCAACTACAGATTGGTTGAAATTGA 7 0.17500000000000002 No Hit GTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCG 7 0.17500000000000002 No Hit GCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACTGGTAAGCCCGTC 7 0.17500000000000002 No Hit GTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCC 7 0.17500000000000002 No Hit AGAAGATTCAGCAGCTACTGCGGCCCCTGCTTCCTCGGGCGGAACTCCAGGT 7 0.17500000000000002 No Hit GCTCAGCCTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGAGG 6 0.15 No Hit CTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTG 6 0.15 No Hit GTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCAACTACA 6 0.15 No Hit CCCTGATCAAACTAGAGGTTACCAAAGAACCATGCATAGCACTGAATAGGGA 6 0.15 No Hit CAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACTGGT 6 0.15 No Hit CGGGAAAGCTGAGGTAGACTTGAGGCCGTTGAATGGTGCAACCATGTTGGCC 6 0.15 No Hit GTCCCTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAAC 5 0.125 No Hit GCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGTACC 5 0.125 No Hit GGGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCG 5 0.125 No Hit GCACGGTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCAA 5 0.125 No Hit GTTCAATTAGATCAGCCCATCAGGCCATGCGGATCCAGGTGGCACCGGCCCG 5 0.125 No Hit CTCGTACACACTAAGAAAAAAGCCTTATCCATTTACAAAAGTCTTATCCATT 5 0.125 No Hit GTTTCCACATAGTCCAGTAGCGTCCATCATAGTACCCTGGGGACTGGTGGTG 5 0.125 No Hit AGCACGGTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra Read 200000 spots for SRR5423395.sra Written 200000 spots for SRR5423395.sra SRR ids: ['SRR5423395.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_uldkeqje SRR5423395.sra spots: 4000000 blocks: [[1, 200000], [200001, 400000], [400001, 600000], [600001, 800000], [800001, 1000000], [1000001, 1200000], [1200001, 1400000], [1400001, 1600000], [1600001, 1800000], [1800001, 2000000], [2000001, 2200000], [2200001, 2400000], [2400001, 2600000], [2600001, 2800000], [2800001, 3000000], [3000001, 3200000], [3200001, 3400000], [3400001, 3600000], [3600001, 3800000], [3800001, 4000000]] SRR5423395 file size 703932 SRR5423395 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423395 SRR5423395_1.fastq Input file: SRR5423395_1.fastq trimmed: SRR5423395-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Thu Feb 13 05:55:12 2025 >> started Thu Feb 13 05:55:14 2025 >> done (2.051s) 4000000 reads processed; of these: 88 ( 0.00%) short reads filtered out after trimming by size control 877 ( 0.02%) empty reads filtered out after trimming by size control 3999035 (99.98%) reads available; of these: 96067 ( 2.40%) trimmed reads available after processing 3902968 (97.60%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 7 0.00% 19 4 0.00% 20 4 0.00% 21 1 0.00% 22 3 0.00% 23 3 0.00% 24 3 0.00% 25 4 0.00% 26 4 0.00% 27 8 0.00% 28 10 0.00% 29 6 0.00% 30 6 0.00% 31 9 0.00% 32 15 0.00% 33 15 0.00% 34 29 0.00% 35 25 0.00% 36 37 0.00% 37 50 0.00% 38 54 0.00% 39 78 0.00% 40 102 0.00% 41 98 0.00% 42 157 0.00% 43 186 0.00% 44 307 0.01% 45 530 0.01% 46 681 0.02% 47 1004 0.03% 48 1842 0.05% 49 3942 0.10% 50 11868 0.30% 51 74975 1.87% 52 3902968 97.60% 3999035 reads passed initial QC criterion=sequence-density sequence-density=0.37 sequence-density-rank=1 fanout-score=1.91 fanout-score-rank=28 prefix-density=0.36 prefix-fanout=1.9 sequence=TGGCACCGGCCCGTAATCCTTCCACTGCCAGGAGCGGGAGGTGATCGCTGCTCTGTGAGACCAGCCTCACGCTGTGCCCTCCCCTCTAGGAGCCACGACACCCAGGTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=16.34 fanout-score-rank=1 prefix-density=0.04 prefix-fanout=3.1 sequence=AAGAAGTTCACACTTTCTGGCGACGTCTTTCTTTTTCTTCAAAACCCCAATCCGCTCTCGCTCGCCGCTACTAACGGGGTCTCGGTTGATTTCCCTTCCTTTAGCTAGTCAGATGTTTCAGTTCGCTAAGTTTGAAAAGTCCAAAGAGCGCAGACTCGCCACGGAGCTTGGAGACGGTTTCCCGATCGGAGATCCATGGATCACAGACGGTATCTCCCCATGGCCTTT Started job on | Feb 13 05:58:00 Started mapping on | Feb 13 05:58:15 Finished on | Feb 13 05:58:35 Mapping speed, Million of reads per hour | 719.83 Number of input reads | 3999035 Average input read length | 51 UNIQUE READS: Uniquely mapped reads number | 3000535 Uniquely mapped reads % | 75.03% Average mapped length | 51.79 Number of splices: Total | 257042 Number of splices: Annotated (sjdb) | 253657 Number of splices: GT/AG | 249161 Number of splices: GC/AG | 6367 Number of splices: AT/AC | 883 Number of splices: Non-canonical | 631 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.01% Deletion average length | 2.33 Insertion rate per base | 0.00% Insertion average length | 1.34 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 847643 % of reads mapped to multiple loci | 21.20% Number of reads mapped to too many loci | 73409 % of reads mapped to too many loci | 1.84% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.82% % of reads unmapped: other | 0.12% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 150857 150857 150857 N_multimapping 847643 847643 847643 N_noFeature 454371 2930687 513303 N_ambiguous 22148 161 11078 UnstrandedReadsAssigned:2524016 PositiveStrandReadsAssigned:69687 NegativeStrandReadsAssigned:2476154 Dataset is classified negative stranded MeadianReadLen=52 20thPercentileLength=52 echo kmer=47 SRR5423395 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR5423395-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,999,035 reads, 3,147,008 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,067 rounds 52401 SRR5423395.ke.tsv 34699 SRR5423395.se.tsv 87100 total ==> SRR5423395.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 93 12.9768 Potri.005G024800.1.v4.1 1035 936 2.00642 0.573993 Potri.004G059700.1.v4.1 961 862 3 0.931911 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 72.5143 6.82738 Potri.016G087400.1.v4.1 270 171 13 20.3567 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 2 0.319915 Potri.012G127500.1.v4.1 977 878 3 0.914928 ==> SRR5423395.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 3 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 26 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 4 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1 SRR5423395 completed mapping pipeline successfully