Starting /dee2/code/volunteer_pipeline.sh SRR5423397 current disk space = 3049644695552 free memory = 1512365064 SRR5423397 SRAfilesize 7c3d7826e36ea212aa827732325c9f68 SRR5423397.sra SRR5423397.sra file validated SRR5423397 is single end SRR5423397 is conventional basespace SRR5423397 read1 length is 52 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5423397_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.4395 34.0 31.0 34.0 28.0 34.0 2 31.53975 34.0 31.0 34.0 27.0 34.0 3 32.3485 34.0 31.0 34.0 28.0 34.0 4 36.02325 37.0 35.0 37.0 35.0 37.0 5 36.096 37.0 35.0 37.0 35.0 37.0 6 36.1245 37.0 36.0 37.0 35.0 37.0 7 36.13675 37.0 35.0 37.0 35.0 37.0 8 36.1385 37.0 36.0 37.0 35.0 37.0 9 37.82375 39.0 38.0 39.0 35.0 39.0 10 37.6135 39.0 37.0 39.0 35.0 39.0 11 37.81275 39.0 38.0 39.0 35.0 39.0 12 37.80525 39.0 38.0 39.0 35.0 39.0 13 37.76525 39.0 38.0 39.0 35.0 39.0 14 39.2355 41.0 39.0 41.0 36.0 41.0 15 39.1705 40.0 39.0 41.0 36.0 41.0 16 39.11375 40.0 39.0 41.0 36.0 41.0 17 39.09075 40.0 38.0 41.0 36.0 41.0 18 39.11325 40.0 38.0 41.0 36.0 41.0 19 39.1455 40.0 39.0 41.0 36.0 41.0 20 38.95375 40.0 39.0 41.0 35.0 41.0 21 38.96625 40.0 39.0 41.0 35.0 41.0 22 38.90525 40.0 38.0 41.0 35.0 41.0 23 38.82725 40.0 38.0 41.0 35.0 41.0 24 38.82425 40.0 38.0 41.0 35.0 41.0 25 38.81325 40.0 38.0 41.0 35.0 41.0 26 38.74 40.0 38.0 41.0 35.0 41.0 27 38.664 40.0 38.0 41.0 35.0 41.0 28 38.48 40.0 38.0 41.0 34.0 41.0 29 38.57675 40.0 38.0 41.0 34.0 41.0 30 38.43475 40.0 38.0 41.0 34.0 41.0 31 38.373 40.0 38.0 41.0 34.0 41.0 32 38.285 40.0 38.0 41.0 34.0 41.0 33 38.2545 40.0 38.0 41.0 34.0 41.0 34 38.32225 40.0 38.0 41.0 34.0 41.0 35 38.225 40.0 38.0 41.0 33.0 41.0 36 38.153 40.0 38.0 41.0 33.0 41.0 37 37.931 40.0 38.0 41.0 33.0 41.0 38 38.0205 40.0 38.0 41.0 33.0 41.0 39 37.79425 40.0 38.0 41.0 33.0 41.0 40 37.5805 40.0 38.0 41.0 31.0 41.0 41 37.61225 40.0 37.0 41.0 32.0 41.0 42 37.543 40.0 37.0 41.0 32.0 41.0 43 37.258 40.0 37.0 41.0 30.0 41.0 44 37.3395 40.0 37.0 41.0 31.0 41.0 45 37.27925 40.0 37.0 41.0 31.0 41.0 46 37.0605 40.0 36.0 41.0 30.0 41.0 47 37.0835 40.0 36.0 41.0 31.0 41.0 48 37.1125 40.0 36.0 41.0 31.0 41.0 49 36.74625 39.0 36.0 41.0 30.0 41.0 50 36.72725 39.0 36.0 41.0 30.0 41.0 51 36.6895 39.0 36.0 41.0 30.0 41.0 52 34.65875 38.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10 0.0 1101 11 0.0 1101 12 0.0 1101 13 0.0 1101 14 0.0 1101 15 0.0 1101 16 0.0 1101 17 0.0 1101 18 0.0 1101 19 0.0 1101 20 0.0 1101 21 0.0 1101 22 0.0 1101 23 0.0 1101 24 0.0 1101 25 0.0 1101 26 0.0 1101 27 0.0 1101 28 0.0 1101 29 0.0 1101 30 0.0 1101 31 0.0 1101 32 0.0 1101 33 0.0 1101 34 0.0 1101 35 0.0 1101 36 0.0 1101 37 0.0 1101 38 0.0 1101 39 0.0 1101 40 0.0 1101 41 0.0 1101 42 0.0 1101 43 0.0 1101 44 0.0 1101 45 0.0 1101 46 0.0 1101 47 0.0 1101 48 0.0 1101 49 0.0 1101 50 0.0 1101 51 0.0 1101 52 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 0.0 14 1.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 1.0 21 2.0 22 6.0 23 6.0 24 11.0 25 15.0 26 20.0 27 28.0 28 26.0 29 46.0 30 44.0 31 56.0 32 87.0 33 113.0 34 144.0 35 198.0 36 257.0 37 468.0 38 800.0 39 1664.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34231805929919 13.180592991913748 5.9838274932614555 37.493261455525605 2 20.9 14.825 35.3 28.975 3 19.85 19.900000000000002 24.975 35.275 4 25.474999999999998 26.974999999999998 20.9 26.650000000000002 5 24.2 32.5 23.5 19.8 6 20.875 35.15 22.675 21.3 7 15.475 23.724999999999998 40.725 20.075000000000003 8 16.650000000000002 22.975 29.95 30.425 9 17.349999999999998 22.6 33.225 26.825 10 19.7 37.574999999999996 23.724999999999998 19.0 11 23.25 28.325 21.025 27.400000000000002 12 21.5 25.45 24.8 28.249999999999996 13 20.25 28.4 27.525 23.825 14 19.75 28.425 26.55 25.275 15 22.3 27.275 24.8 25.624999999999996 16 21.675 25.674999999999997 26.700000000000003 25.95 17 21.425 27.1 26.075 25.4 18 21.05 27.400000000000002 25.650000000000002 25.900000000000002 19 21.85 28.475 23.9 25.775 20 22.075 26.224999999999998 26.5 25.2 21 21.425 27.6 24.3 26.674999999999997 22 21.475 27.6 24.825 26.1 23 21.4 28.849999999999998 25.1 24.65 24 22.075 27.150000000000002 24.6 26.174999999999997 25 22.625 28.499999999999996 24.075 24.8 26 20.925 27.525 25.85 25.7 27 21.05 27.200000000000003 25.424999999999997 26.325 28 23.125 27.35 26.575 22.95 29 20.724999999999998 26.75 27.725 24.8 30 20.525 26.825 25.7 26.950000000000003 31 21.75 26.424999999999997 25.25 26.575 32 20.549999999999997 27.675 24.75 27.025 33 20.45 26.375 27.075 26.1 34 21.25 27.05 26.3 25.4 35 21.275 26.85 24.375 27.500000000000004 36 20.474999999999998 28.025 24.5 27.0 37 22.375 26.575 25.75 25.3 38 22.650000000000002 26.1 24.75 26.5 39 21.55 26.650000000000002 24.875 26.924999999999997 40 22.25 27.950000000000003 26.125 23.674999999999997 41 22.075 26.55 24.975 26.400000000000002 42 20.125 26.0 26.25 27.625 43 23.65 27.0 24.55 24.8 44 21.9 26.674999999999997 25.4 26.025 45 21.325 27.500000000000004 25.900000000000002 25.275 46 24.0 27.6 24.375 24.025 47 23.7 27.125 24.15 25.025 48 22.3 26.525 24.425 26.75 49 22.25 27.875 24.4 25.474999999999998 50 23.625 27.650000000000002 23.625 25.1 51 22.400000000000002 26.025 24.75 26.825 52 23.974999999999998 27.1 24.7 24.224999999999998 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5.0 1 3.0 2 1.0 3 1.5 4 2.0 5 1.5 6 1.0 7 1.5 8 2.0 9 2.5 10 3.0 11 1.5 12 0.0 13 0.0 14 0.0 15 0.0 16 1.5 17 3.0 18 4.0 19 5.0 20 7.5 21 10.0 22 10.5 23 11.0 24 15.0 25 19.0 26 18.5 27 18.0 28 26.0 29 34.0 30 47.5 31 61.0 32 75.0 33 89.0 34 110.5 35 132.0 36 160.0 37 188.0 38 184.5 39 205.0 40 229.0 41 231.0 42 233.0 43 263.0 44 293.0 45 294.0 46 295.0 47 301.0 48 307.0 49 310.5 50 314.0 51 327.0 52 340.0 53 327.5 54 315.0 55 272.5 56 230.0 57 219.0 58 208.0 59 184.0 60 160.0 61 136.5 62 113.0 63 94.0 64 63.0 65 51.0 66 40.5 67 30.0 68 22.5 69 15.0 70 12.5 71 10.0 72 8.0 73 6.0 74 5.5 75 5.0 76 3.5 77 2.0 78 2.0 79 2.0 80 1.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 7.249999999999999 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.85 #Duplication Level Percentage of deduplicated Percentage of total 1 93.47214406302757 83.05 2 3.995498030388295 7.1 3 1.1536297129994373 3.075 4 0.5064715813168261 1.7999999999999998 5 0.39392234102419804 1.7500000000000002 6 0.22509848058525606 1.2 7 0.028137310073157007 0.17500000000000002 8 0.11254924029262803 0.8 9 0.028137310073157007 0.22499999999999998 >10 0.08441193021947102 0.8250000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGC 12 0.3 No Hit GGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGA 11 0.27499999999999997 No Hit GTCCCTTATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCA 10 0.25 No Hit CCAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACTGG 9 0.22499999999999998 No Hit GTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCAACTACA 8 0.2 No Hit CACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCCCTG 8 0.2 No Hit GCCTAACATGTGAAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACA 8 0.2 No Hit CCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACT 8 0.2 No Hit ATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCATGTACCA 7 0.17500000000000002 No Hit CTCGTATTTTCCCCTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTG 6 0.15 No Hit GCCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTAC 6 0.15 No Hit GATCAAACTAGAGGTTACCAAAGAACCATGCATAGCACTGAATAGGGAGCCG 6 0.15 No Hit CCCAGGTATTAATTACACGACCTTGACTATCAACTACAGATTGGTTGAAATT 6 0.15 No Hit CTTATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCATGTA 6 0.15 No Hit GTTCAATTAGATCAGCCCATCAGGCCATGCGGATCCAGGTGGCACCGGCCCG 6 0.15 No Hit GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGTA 6 0.15 No Hit CCGCATTTCGCTAAAGGGTTGAAGGAGGATAGTGCATCAAGCTGTTCGCAAG 6 0.15 No Hit GGGCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGAT 5 0.125 No Hit CCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCCCT 5 0.125 No Hit GGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGT 5 0.125 No Hit GTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCG 5 0.125 No Hit CACGGTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCAAC 5 0.125 No Hit GGTCGGTCCATGGATTTTCCTTCCTTTTTCCGCATTTCGCTAAAGGGTTGAA 5 0.125 No Hit CAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACTGGT 5 0.125 No Hit CCAGGTATTAATTACACGACCTTGACTATCAACTACAGATTGGTTGAAATTG 5 0.125 No Hit GTACACACTAAGAAAAAAGCCTTATCCATTTACAAAAGTCTTATCCATTTGT 5 0.125 No Hit GTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCC 5 0.125 No Hit CTCCAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACT 5 0.125 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.125 No Hit GCCGAATACACCAGCTACGCCTAACATGTGAAATGGGTGCATAAGGATGTTG 5 0.125 No Hit GCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGATCA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra Read 200000 spots for SRR5423397.sra Written 200000 spots for SRR5423397.sra SRR ids: ['SRR5423397.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_wg7gtw6y SRR5423397.sra spots: 4000000 blocks: [[1, 200000], [200001, 400000], [400001, 600000], [600001, 800000], [800001, 1000000], [1000001, 1200000], [1200001, 1400000], [1400001, 1600000], [1600001, 1800000], [1800001, 2000000], [2000001, 2200000], [2200001, 2400000], [2400001, 2600000], [2600001, 2800000], [2800001, 3000000], [3000001, 3200000], [3200001, 3400000], [3400001, 3600000], [3600001, 3800000], [3800001, 4000000]] SRR5423397 file size 703995 SRR5423397 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423397 SRR5423397_1.fastq Input file: SRR5423397_1.fastq trimmed: SRR5423397-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Wed Feb 12 09:26:47 2025 >> started Wed Feb 12 09:26:49 2025 >> done (1.948s) 4000000 reads processed; of these: 89 ( 0.00%) short reads filtered out after trimming by size control 815 ( 0.02%) empty reads filtered out after trimming by size control 3999096 (99.98%) reads available; of these: 85500 ( 2.14%) trimmed reads available after processing 3913596 (97.86%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 15 0.00% 19 5 0.00% 20 9 0.00% 21 2 0.00% 22 2 0.00% 23 1 0.00% 24 3 0.00% 25 6 0.00% 26 6 0.00% 27 9 0.00% 28 6 0.00% 29 8 0.00% 30 9 0.00% 31 13 0.00% 32 15 0.00% 33 14 0.00% 34 26 0.00% 35 29 0.00% 36 35 0.00% 37 45 0.00% 38 59 0.00% 39 59 0.00% 40 82 0.00% 41 131 0.00% 42 145 0.00% 43 182 0.00% 44 277 0.01% 45 412 0.01% 46 569 0.01% 47 874 0.02% 48 1545 0.04% 49 3336 0.08% 50 10280 0.26% 51 67291 1.68% 52 3913596 97.86% 3999096 reads passed initial QC criterion=sequence-density sequence-density=0.39 sequence-density-rank=1 fanout-score=1.91 fanout-score-rank=27 prefix-density=0.37 prefix-fanout=1.9 sequence=TGGCACCGGCCCGTAATCCTTCCACTGCCAGGAGCGGGAGGTGATCGCTGCTCTGTGAGACCAGCCTCACGCTGTGCCCTCCCCTCTAGGAGCCACGACACC criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=19.31 fanout-score-rank=1 prefix-density=0.04 prefix-fanout=3.1 sequence=AAGAAGTTCACACTTTCTGGCGACGTCTTTCTTTTTCTTCAAAACCCCAATCCGCTCTCGCTCGCCGCTACTAACGGGGTCTCGGTTGATTTCCCTTCCTTTAGCTAGTCAGATGTTTCAGTTCGCTAAGTTTGAAAAGTCCAAAGAGCGCAGACTCGCCACGGAGCTTGGAGACGGTTTCCCGATCGGAGATCCATGGATCACAGACGGTATCTCCCCATGGCCTTTC Started job on | Feb 12 09:27:02 Started mapping on | Feb 12 09:27:02 Finished on | Feb 12 09:27:07 Mapping speed, Million of reads per hour | 2879.35 Number of input reads | 3999096 Average input read length | 51 UNIQUE READS: Uniquely mapped reads number | 3007087 Uniquely mapped reads % | 75.19% Average mapped length | 51.80 Number of splices: Total | 258564 Number of splices: Annotated (sjdb) | 255333 Number of splices: GT/AG | 250710 Number of splices: GC/AG | 6374 Number of splices: AT/AC | 824 Number of splices: Non-canonical | 656 Mismatch rate per base, % | 0.34% Deletion rate per base | 0.01% Deletion average length | 2.34 Insertion rate per base | 0.00% Insertion average length | 1.34 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 840378 % of reads mapped to multiple loci | 21.01% Number of reads mapped to too many loci | 74926 % of reads mapped to too many loci | 1.87% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.81% % of reads unmapped: other | 0.11% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 151631 151631 151631 N_multimapping 840378 840378 840378 N_noFeature 455397 2935981 515637 N_ambiguous 21929 141 10935 UnstrandedReadsAssigned:2529761 PositiveStrandReadsAssigned:70965 NegativeStrandReadsAssigned:2480515 Dataset is classified negative stranded MeadianReadLen=52 20thPercentileLength=52 echo kmer=47 SRR5423397 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR5423397-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,999,096 reads, 3,159,123 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,042 rounds 52401 SRR5423397.ke.tsv 34699 SRR5423397.se.tsv 87100 total ==> SRR5423397.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 77 10.6756 Potri.005G024800.1.v4.1 1035 936 8 2.27401 Potri.004G059700.1.v4.1 961 862 7 2.16057 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 67.7408 6.33722 Potri.016G087400.1.v4.1 270 171 17 26.4503 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 2 0.317872 Potri.012G127500.1.v4.1 977 878 10 3.03029 ==> SRR5423397.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 5 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 32 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 2 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR5423397 completed mapping pipeline successfully