Starting /dee2/code/volunteer_pipeline.sh SRR5423406 current disk space = 3053174677504 free memory = 1579482216 SRR5423406 SRAfilesize 7150b28dbba757a97eb9ad717f5982db SRR5423406.sra SRR5423406.sra file validated SRR5423406 is single end SRR5423406 is conventional basespace SRR5423406 read1 length is 52 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5423406_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.46225 31.0 30.0 33.0 26.0 34.0 2 31.094 31.0 30.0 34.0 28.0 34.0 3 31.6025 31.0 31.0 34.0 30.0 34.0 4 28.7665 33.0 22.0 35.0 10.0 37.0 5 33.12525 35.0 32.0 37.0 28.0 37.0 6 34.536 35.0 35.0 37.0 31.0 37.0 7 34.87125 35.0 35.0 37.0 32.0 37.0 8 35.0765 36.0 35.0 37.0 32.0 37.0 9 36.81175 39.0 37.0 39.0 32.0 39.0 10 36.68925 39.0 37.0 39.0 32.0 39.0 11 37.046 39.0 37.0 39.0 33.0 39.0 12 36.88275 39.0 37.0 39.0 33.0 39.0 13 36.73425 39.0 37.0 39.0 32.0 39.0 14 37.33475 39.0 36.0 41.0 32.0 41.0 15 37.73275 40.0 37.0 41.0 32.0 41.0 16 37.508 39.0 36.0 41.0 32.0 41.0 17 37.82775 40.0 37.0 41.0 33.0 41.0 18 37.46475 39.0 36.0 41.0 32.0 41.0 19 38.03575 40.0 37.0 41.0 33.0 41.0 20 37.7265 40.0 37.0 41.0 32.0 41.0 21 37.97725 40.0 37.0 41.0 33.0 41.0 22 38.02975 40.0 37.0 41.0 33.0 41.0 23 37.8135 40.0 37.0 41.0 32.0 41.0 24 37.73925 40.0 37.0 41.0 32.0 41.0 25 37.806 40.0 37.0 41.0 32.0 41.0 26 37.57725 40.0 37.0 41.0 32.0 41.0 27 37.6275 40.0 37.0 41.0 32.0 41.0 28 37.59325 40.0 37.0 41.0 32.0 41.0 29 37.75375 40.0 37.0 41.0 32.0 41.0 30 37.846 40.0 37.0 41.0 33.0 41.0 31 37.77475 40.0 37.0 41.0 33.0 41.0 32 37.74775 40.0 37.0 41.0 33.0 41.0 33 37.73575 40.0 37.0 41.0 32.0 41.0 34 37.55625 40.0 37.0 41.0 32.0 41.0 35 37.42975 40.0 36.0 41.0 31.0 41.0 36 37.462 40.0 37.0 41.0 31.0 41.0 37 37.33125 39.0 36.0 41.0 31.0 41.0 38 37.0695 39.0 36.0 41.0 30.0 41.0 39 36.90925 39.0 36.0 41.0 30.0 41.0 40 37.20925 39.0 36.0 41.0 31.0 41.0 41 37.258 39.0 36.0 41.0 31.0 41.0 42 37.36775 39.0 37.0 41.0 31.0 41.0 43 37.0525 39.0 36.0 40.0 31.0 41.0 44 37.06375 39.0 36.0 40.0 31.0 41.0 45 36.7445 39.0 35.0 40.0 30.0 41.0 46 36.6415 39.0 35.0 40.0 30.0 41.0 47 36.62175 39.0 35.0 40.0 30.0 41.0 48 36.7745 39.0 35.0 40.0 30.0 41.0 49 36.54025 39.0 35.0 40.0 29.0 41.0 50 36.53125 39.0 35.0 40.0 30.0 41.0 51 36.39225 39.0 35.0 40.0 29.0 41.0 52 35.97175 38.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2215 1 0.0 2215 2 0.0 2215 3 0.0 2215 4 0.0 2215 5 0.0 2215 6 0.0 2215 7 0.0 2215 8 0.0 2215 9 0.0 2215 10 0.0 2215 11 0.0 2215 12 0.0 2215 13 0.0 2215 14 0.0 2215 15 0.0 2215 16 0.0 2215 17 0.0 2215 18 0.0 2215 19 0.0 2215 20 0.0 2215 21 0.0 2215 22 0.0 2215 23 0.0 2215 24 0.0 2215 25 0.0 2215 26 0.0 2215 27 0.0 2215 28 0.0 2215 29 0.0 2215 30 0.0 2215 31 0.0 2215 32 0.0 2215 33 0.0 2215 34 0.0 2215 35 0.0 2215 36 0.0 2215 37 0.0 2215 38 0.0 2215 39 0.0 2215 40 0.0 2215 41 0.0 2215 42 0.0 2215 43 0.0 2215 44 0.0 2215 45 0.0 2215 46 0.0 2215 47 0.0 2215 48 0.0 2215 49 0.0 2215 50 0.0 2215 51 0.0 2215 52 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 0.0 14 0.0 15 0.0 16 0.0 17 1.0 18 0.0 19 1.0 20 1.0 21 1.0 22 2.0 23 4.0 24 12.0 25 14.0 26 28.0 27 30.0 28 52.0 29 67.0 30 84.0 31 116.0 32 151.0 33 178.0 34 221.0 35 285.0 36 363.0 37 613.0 38 863.0 39 911.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.179179179179176 10.635635635635635 6.856856856856856 53.32832832832833 2 22.1 13.25 37.4 27.250000000000004 3 21.275 17.625 23.775 37.325 4 25.900000000000002 23.425 23.425 27.250000000000004 5 24.5 29.975 25.525 20.0 6 19.525000000000002 33.125 26.05 21.3 7 14.899999999999999 26.275 39.75 19.075 8 18.925 22.675 30.275000000000002 28.125 9 16.1 20.525 36.275 27.1 10 17.25 38.625 23.599999999999998 20.525 11 21.75 28.975 21.85 27.425 12 22.775000000000002 23.45 25.4 28.375 13 20.150000000000002 27.275 27.075 25.5 14 20.424999999999997 28.599999999999998 26.275 24.7 15 21.9 27.075 26.650000000000002 24.375 16 20.125 28.475 25.924999999999997 25.474999999999998 17 21.975 26.674999999999997 27.275 24.075 18 20.95 26.724999999999998 27.3 25.025 19 20.7 27.575 25.724999999999998 26.0 20 21.7 27.925 25.124999999999996 25.25 21 20.075000000000003 27.500000000000004 26.450000000000003 25.974999999999998 22 21.5 28.175 24.675 25.650000000000002 23 20.825 28.599999999999998 24.775 25.8 24 22.2 27.950000000000003 24.2 25.650000000000002 25 22.400000000000002 27.35 25.674999999999997 24.575 26 21.7 28.025 25.825 24.45 27 21.099999999999998 27.750000000000004 27.375 23.775 28 20.349999999999998 27.750000000000004 25.924999999999997 25.974999999999998 29 21.15 28.675 26.424999999999997 23.75 30 20.325 26.224999999999998 26.5 26.950000000000003 31 21.375 28.225 25.624999999999996 24.775 32 21.25 26.5 26.875 25.374999999999996 33 20.599999999999998 26.375 26.150000000000002 26.875 34 21.425 27.625 25.6 25.35 35 21.475 26.224999999999998 25.924999999999997 26.375 36 21.475 25.674999999999997 26.125 26.724999999999998 37 20.75 27.85 25.874999999999996 25.525 38 22.875 26.325 25.874999999999996 24.925 39 20.825 27.05 26.224999999999998 25.900000000000002 40 20.9 26.55 25.5 27.05 41 20.75 26.200000000000003 26.0 27.05 42 20.75 26.674999999999997 27.0 25.575 43 21.75 27.325 24.9 26.025 44 21.325 27.950000000000003 26.325 24.4 45 21.7 26.275 26.6 25.424999999999997 46 23.549999999999997 28.025 24.0 24.425 47 23.0 27.55 24.3 25.15 48 21.925 27.0 25.05 26.025 49 20.549999999999997 26.1 26.0 27.35 50 22.3 28.050000000000004 24.95 24.7 51 22.575 24.474999999999998 26.1 26.85 52 22.075 26.125 26.875 24.925 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 1.0 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 1.0 15 0.0 16 1.5 17 3.0 18 3.0 19 3.0 20 5.0 21 7.0 22 7.0 23 7.0 24 7.5 25 8.0 26 15.0 27 22.0 28 31.0 29 40.0 30 45.0 31 50.0 32 63.5 33 77.0 34 99.0 35 121.0 36 150.0 37 179.0 38 205.0 39 241.0 40 251.0 41 262.0 42 273.0 43 288.5 44 304.0 45 310.0 46 316.0 47 332.5 48 349.0 49 336.0 50 323.0 51 320.5 52 318.0 53 321.0 54 324.0 55 274.5 56 225.0 57 209.5 58 194.0 59 165.0 60 136.0 61 117.5 62 99.0 63 76.0 64 43.5 65 34.0 66 30.0 67 26.0 68 17.5 69 9.0 70 6.5 71 4.0 72 6.5 73 9.0 74 4.5 75 0.0 76 0.5 77 1.0 78 1.0 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.325 #Duplication Level Percentage of deduplicated Percentage of total 1 93.75874615169324 83.75 2 3.918275958578225 7.000000000000001 3 1.0075566750629723 2.7 4 0.5037783375314862 1.7999999999999998 5 0.30786453960257487 1.375 6 0.22390148334732718 1.2 7 0.11195074167366359 0.7000000000000001 8 0.08396305625524769 0.6 9 0.027987685418415897 0.22499999999999998 >10 0.055975370836831795 0.65 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACT 15 0.375 No Hit CGCAGAAGTAGGAATAATGGCACCAGAAATGATATTGTTTCCATAAAGTAGA 11 0.27499999999999997 No Hit CTCGTATTTTCCCCTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTG 9 0.22499999999999998 No Hit GGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGA 8 0.2 No Hit CGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGC 8 0.2 No Hit CTCCAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACT 8 0.2 No Hit GTCCCTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAAC 7 0.17500000000000002 No Hit GTCCGCGACCCCTGGATGCCGAAGGCGTCCTTGGGGCGATCTCGTAGTTCCT 7 0.17500000000000002 No Hit CTTATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCATGTA 7 0.17500000000000002 No Hit AGCACGGTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCA 7 0.17500000000000002 No Hit GGGCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGAT 6 0.15 No Hit CTCGGGTCCTAGAAGTGCATGATAAATACCGCCAAAGCCCAATACTGCAGAG 6 0.15 No Hit CGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTG 6 0.15 No Hit GCCCCATGTAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 6 0.15 No Hit CCCTGATCAAACTAGAGGTTACCAAAGAACCATGCATAGCACTGAATAGGGA 6 0.15 No Hit CCCGAAGCCACGGGTAGAACACCCGGTAGAGAGACCCAATCTTGAGTGAAAT 6 0.15 No Hit GTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGA 6 0.15 No Hit CTCGTACACACTAAGAAAAAAGCCTTATCCATTTACAAAAGTCTTATCCATT 6 0.15 No Hit GCCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTAC 5 0.125 No Hit CTTAAGAATGCTGGTTTTAAGAATGAGTGATTGCCCTTCTCCGACCCTTACT 5 0.125 No Hit CTCACGAGCAAGATCACGTCCCTCATTACGAGCTTGTACACATGCTTCTAGA 5 0.125 No Hit CTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGCCC 5 0.125 No Hit GCCAGACTAAGCAATAAAGTACCTCGTATTTTCCCCTCTGCCTCGGGTTGTC 5 0.125 No Hit CGATTAGCAACGGCACCGGGTGCATTTCCCCAAGGGTGCCCTAAAGTTCCTC 5 0.125 No Hit CTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAACGGCA 5 0.125 No Hit CAAGTATTTAACACGGTCTGGTCCGTACCATGGGCTCCCGGAGGGAACAGGC 5 0.125 No Hit CCCACTTTTTGGTCTTAAGAATGCTGGTTTTAAGAATGAGTGATTGCCCTTC 5 0.125 No Hit GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGTA 5 0.125 No Hit CTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTGGCCCGCAGCAGTA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10 0.025 0.0 0.0 0.0 0.0 11 0.025 0.0 0.0 0.0 0.0 12 0.025 0.0 0.0 0.0 0.0 13 0.025 0.0 0.0 0.0 0.0 14 0.025 0.0 0.0 0.0 0.0 15 0.025 0.0 0.0 0.0 0.0 16 0.025 0.0 0.0 0.0 0.0 17 0.025 0.0 0.0 0.0 0.0 18 0.025 0.0 0.0 0.0 0.0 19 0.025 0.0 0.0 0.0 0.0 20 0.025 0.0 0.0 0.0 0.0 21 0.025 0.0 0.0 0.0 0.0 22 0.025 0.0 0.0 0.0 0.0 23 0.025 0.0 0.0 0.0 0.0 24 0.025 0.0 0.0 0.0 0.0 25 0.025 0.0 0.0 0.0 0.0 26 0.025 0.0 0.0 0.0 0.0 27 0.025 0.0 0.0 0.0 0.0 28 0.025 0.0 0.0 0.0 0.0 29 0.025 0.0 0.0 0.0 0.0 30 0.025 0.0 0.0 0.0 0.0 31 0.025 0.0 0.0 0.0 0.0 32 0.025 0.0 0.0 0.0 0.0 33 0.025 0.0 0.0 0.0 0.0 34 0.025 0.0 0.0 0.0 0.0 35 0.025 0.0 0.0 0.0 0.0 36 0.025 0.0 0.0 0.0 0.0 37 0.025 0.0 0.0 0.0 0.0 38 0.025 0.0 0.0 0.0 0.0 39 0.025 0.0 0.0 0.0 0.0 40 0.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra Read 200000 spots for SRR5423406.sra Written 200000 spots for SRR5423406.sra SRR ids: ['SRR5423406.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_fzwi_xqp SRR5423406.sra spots: 4000000 blocks: [[1, 200000], [200001, 400000], [400001, 600000], [600001, 800000], [800001, 1000000], [1000001, 1200000], [1200001, 1400000], [1400001, 1600000], [1600001, 1800000], [1800001, 2000000], [2000001, 2200000], [2200001, 2400000], [2400001, 2600000], [2600001, 2800000], [2800001, 3000000], [3000001, 3200000], [3200001, 3400000], [3400001, 3600000], [3600001, 3800000], [3800001, 4000000]] SRR5423406 file size 703931 SRR5423406 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423406 SRR5423406_1.fastq Input file: SRR5423406_1.fastq trimmed: SRR5423406-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Thu Feb 13 06:18:53 2025 >> started Thu Feb 13 06:18:55 2025 >> done (2.092s) 4000000 reads processed; of these: 150 ( 0.00%) short reads filtered out after trimming by size control 42 ( 0.00%) empty reads filtered out after trimming by size control 3999808 (100.00%) reads available; of these: 67373 ( 1.68%) trimmed reads available after processing 3932435 (98.32%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 4 0.00% 20 5 0.00% 21 0 0.00% 22 0 0.00% 23 1 0.00% 24 1 0.00% 25 6 0.00% 26 1 0.00% 27 1 0.00% 28 4 0.00% 29 3 0.00% 30 6 0.00% 31 4 0.00% 32 9 0.00% 33 9 0.00% 34 9 0.00% 35 10 0.00% 36 28 0.00% 37 18 0.00% 38 29 0.00% 39 37 0.00% 40 47 0.00% 41 77 0.00% 42 111 0.00% 43 107 0.00% 44 352 0.01% 45 433 0.01% 46 568 0.01% 47 666 0.02% 48 1304 0.03% 49 3253 0.08% 50 8297 0.21% 51 51967 1.30% 52 3932435 98.32% 3999808 reads passed initial QC criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=3.68 fanout-score-rank=11 prefix-density=0.42 prefix-fanout=3.0 sequence=CCATTGCTTGCAATGGAAGTAATGTCATTAGCCTTTCT criterion=fanout-score sequence-density=0.01 sequence-density-rank=30 fanout-score=27.25 fanout-score-rank=1 prefix-density=0.36 prefix-fanout=1.1 sequence=CATTTTATTTCTATCTTTCCATACATAACCAAAAAATGGAAAAGATTCTTCAAGAGTCTCGGGTCCTAGAAGTGCATGATAAATACCGCCAAAGCCCAATACTGCAGAGGAAATTAAGTGAAGTACGCCAGATACAAAGTATGGAAAGGTGTCTATGACTTCCCCGCCAGGACCCACCCCCCAACCTAGAGTAGCTAGGTGGGGAAGTAAAATTAATCCTTGTTCATACATTGGCTTCTCCGGTACGAAATGGGCCACTTCAAATAGGTTCATTGCTCCGGCCCAGAATACGATTAATCCAGCATGGGCTACATGAGCTCCCAGCAG Started job on | Feb 13 06:19:12 Started mapping on | Feb 13 06:19:14 Finished on | Feb 13 06:19:19 Mapping speed, Million of reads per hour | 2879.86 Number of input reads | 3999808 Average input read length | 51 UNIQUE READS: Uniquely mapped reads number | 3117843 Uniquely mapped reads % | 77.95% Average mapped length | 51.81 Number of splices: Total | 277459 Number of splices: Annotated (sjdb) | 274013 Number of splices: GT/AG | 269551 Number of splices: GC/AG | 6368 Number of splices: AT/AC | 1057 Number of splices: Non-canonical | 483 Mismatch rate per base, % | 0.33% Deletion rate per base | 0.01% Deletion average length | 2.75 Insertion rate per base | 0.00% Insertion average length | 1.32 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 764976 % of reads mapped to multiple loci | 19.13% Number of reads mapped to too many loci | 54854 % of reads mapped to too many loci | 1.37% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.54% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 116989 116989 116989 N_multimapping 764976 764976 764976 N_noFeature 404006 3077228 433322 N_ambiguous 22553 158 11104 UnstrandedReadsAssigned:2691284 PositiveStrandReadsAssigned:40457 NegativeStrandReadsAssigned:2673417 Dataset is classified negative stranded MeadianReadLen=52 20thPercentileLength=52 echo kmer=47 SRR5423406 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR5423406-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,999,808 reads, 3,267,669 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,065 rounds 52401 SRR5423406.ke.tsv 34699 SRR5423406.se.tsv 87100 total ==> SRR5423406.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 83 11.6579 Potri.005G024800.1.v4.1 1035 936 2.0074 0.578064 Potri.004G059700.1.v4.1 961 862 2 0.625375 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 73.826 6.99676 Potri.016G087400.1.v4.1 270 171 15 23.6436 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 0 0 Potri.012G127500.1.v4.1 977 878 5 1.53495 ==> SRR5423406.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 1 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 23 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 6 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR5423406 completed mapping pipeline successfully