Starting /dee2/code/volunteer_pipeline.sh SRR5423412 current disk space = 3052650196992 free memory = 1582193512 SRR5423412 SRAfilesize 33e4e92c23fbe2c90ab14ae3e1dc45ef SRR5423412.sra SRR5423412.sra file validated SRR5423412 is single end SRR5423412 is conventional basespace SRR5423412 read1 length is 52 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5423412_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.2405 31.0 31.0 34.0 28.0 34.0 2 31.3315 31.0 31.0 34.0 27.0 34.0 3 31.734 31.0 31.0 34.0 30.0 34.0 4 32.8975 35.0 33.0 37.0 22.0 37.0 5 34.62225 35.0 35.0 37.0 32.0 37.0 6 34.99925 36.0 35.0 37.0 32.0 37.0 7 35.16375 37.0 35.0 37.0 32.0 37.0 8 35.22 37.0 35.0 37.0 32.0 37.0 9 36.9595 39.0 37.0 39.0 33.0 39.0 10 36.7215 39.0 35.0 39.0 32.0 39.0 11 36.85525 39.0 37.0 39.0 32.0 39.0 12 36.86325 39.0 37.0 39.0 32.0 39.0 13 36.84975 39.0 37.0 39.0 33.0 39.0 14 37.925 40.0 37.0 41.0 33.0 41.0 15 37.981 40.0 37.0 41.0 33.0 41.0 16 37.22775 39.0 36.0 41.0 31.0 41.0 17 37.60375 39.0 36.0 41.0 32.0 41.0 18 37.7605 40.0 37.0 41.0 32.0 41.0 19 37.69725 40.0 37.0 41.0 32.0 41.0 20 37.71525 40.0 37.0 41.0 32.0 41.0 21 37.8795 40.0 37.0 41.0 32.0 41.0 22 37.73675 40.0 37.0 41.0 32.0 41.0 23 37.89075 40.0 37.0 41.0 33.0 41.0 24 37.782 40.0 37.0 41.0 32.0 41.0 25 37.7485 40.0 37.0 41.0 32.0 41.0 26 37.78375 40.0 37.0 41.0 32.0 41.0 27 37.7985 40.0 37.0 41.0 32.0 41.0 28 37.8205 40.0 37.0 41.0 33.0 41.0 29 37.76675 40.0 37.0 41.0 33.0 41.0 30 37.7125 40.0 37.0 41.0 32.0 41.0 31 37.635 40.0 37.0 41.0 32.0 41.0 32 37.6085 40.0 37.0 41.0 32.0 41.0 33 37.5735 40.0 37.0 41.0 32.0 41.0 34 37.5605 40.0 36.0 41.0 32.0 41.0 35 37.66625 40.0 37.0 41.0 32.0 41.0 36 37.47425 40.0 37.0 41.0 31.0 41.0 37 37.19925 39.0 36.0 41.0 31.0 41.0 38 37.35175 39.0 36.0 41.0 31.0 41.0 39 37.23175 39.0 36.0 41.0 31.0 41.0 40 36.845 39.0 36.0 41.0 30.0 41.0 41 37.008 39.0 36.0 41.0 31.0 41.0 42 37.123 39.0 36.0 41.0 31.0 41.0 43 36.96125 39.0 36.0 40.0 30.0 41.0 44 36.811 39.0 35.0 40.0 30.0 41.0 45 36.8215 39.0 35.0 40.0 30.0 41.0 46 36.761 39.0 35.0 40.0 30.0 41.0 47 36.7655 39.0 35.0 40.0 30.0 41.0 48 36.31725 38.0 35.0 40.0 29.0 41.0 49 36.33825 39.0 35.0 40.0 29.0 41.0 50 36.66675 39.0 35.0 40.0 30.0 41.0 51 36.54075 39.0 35.0 40.0 30.0 41.0 52 35.5535 38.0 34.0 40.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1313 1 0.0 1313 2 0.0 1313 3 0.0 1313 4 0.0 1313 5 0.0 1313 6 0.0 1313 7 0.0 1313 8 0.0 1313 9 0.0 1313 10 0.0 1313 11 0.0 1313 12 0.0 1313 13 0.0 1313 14 0.0 1313 15 0.0 1313 16 0.0 1313 17 0.0 1313 18 0.0 1313 19 0.0 1313 20 0.0 1313 21 0.0 1313 22 0.0 1313 23 0.0 1313 24 0.0 1313 25 0.0 1313 26 0.0 1313 27 0.0 1313 28 0.0 1313 29 0.0 1313 30 0.0 1313 31 0.0 1313 32 0.0 1313 33 0.0 1313 34 0.0 1313 35 0.0 1313 36 0.0 1313 37 0.0 1313 38 0.0 1313 39 0.0 1313 40 0.0 1313 41 0.0 1313 42 0.0 1313 43 0.0 1313 44 0.0 1313 45 0.0 1313 46 0.0 1313 47 0.0 1313 48 0.0 1313 49 0.0 1313 50 0.0 1313 51 0.0 1313 52 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 3.0 22 3.0 23 3.0 24 7.0 25 15.0 26 26.0 27 28.0 28 37.0 29 64.0 30 86.0 31 130.0 32 144.0 33 181.0 34 236.0 35 277.0 36 399.0 37 489.0 38 738.0 39 1130.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.65 10.299999999999999 6.775 53.27499999999999 2 22.225 13.825000000000001 36.449999999999996 27.500000000000004 3 22.325 16.45 22.675 38.550000000000004 4 24.95 25.2 20.349999999999998 29.5 5 24.25 29.9 25.25 20.599999999999998 6 19.0 34.425 24.375 22.2 7 14.774999999999999 24.925 39.900000000000006 20.4 8 18.45 22.0 31.525 28.025 9 18.75 22.7 32.925 25.624999999999996 10 17.75 38.525 24.125 19.6 11 23.275000000000002 27.725 22.125 26.875 12 21.125 24.8 27.224999999999998 26.85 13 20.200000000000003 27.775 25.874999999999996 26.150000000000002 14 20.875 27.6 27.0 24.525 15 20.7 27.425 27.025 24.85 16 20.875 28.199999999999996 26.724999999999998 24.2 17 21.675 27.775 26.325 24.224999999999998 18 21.475 27.875 25.575 25.074999999999996 19 19.7 28.325 25.424999999999997 26.55 20 20.925 27.950000000000003 25.424999999999997 25.7 21 20.599999999999998 27.55 26.700000000000003 25.15 22 21.3 28.025 23.775 26.900000000000002 23 21.075 28.549999999999997 26.200000000000003 24.175 24 21.025 27.35 25.374999999999996 26.25 25 22.0 26.575 24.9 26.525 26 22.0 27.675 25.650000000000002 24.675 27 20.025000000000002 27.825 27.500000000000004 24.65 28 20.525 27.950000000000003 26.825 24.7 29 19.875 27.474999999999998 28.249999999999996 24.4 30 21.125 25.85 25.374999999999996 27.650000000000002 31 19.975 29.2 25.374999999999996 25.45 32 20.225 26.450000000000003 26.174999999999997 27.150000000000002 33 20.05 26.575 27.3 26.075 34 20.075000000000003 27.700000000000003 26.650000000000002 25.575 35 21.0 26.650000000000002 26.200000000000003 26.150000000000002 36 21.4 25.95 24.7 27.950000000000003 37 20.3 27.3 27.0 25.4 38 20.575 26.650000000000002 26.950000000000003 25.825 39 20.75 24.5 25.924999999999997 28.825 40 21.925 26.200000000000003 25.85 26.025 41 21.7 26.375 25.674999999999997 26.25 42 20.125 26.224999999999998 27.200000000000003 26.450000000000003 43 21.2 27.150000000000002 25.674999999999997 25.974999999999998 44 22.825 26.700000000000003 24.875 25.6 45 21.980495123780948 27.25681420355089 25.656414103525883 25.10627656914228 46 22.775000000000002 26.625 24.349999999999998 26.25 47 23.325000000000003 28.125 24.349999999999998 24.2 48 21.675 27.0 25.45 25.874999999999996 49 22.175 25.674999999999997 25.0 27.150000000000002 50 22.3 28.599999999999998 24.875 24.224999999999998 51 22.475 25.95 24.925 26.650000000000002 52 23.599999999999998 26.125 25.724999999999998 24.55 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.5 15 2.0 16 2.5 17 3.0 18 4.0 19 5.0 20 5.0 21 5.0 22 5.5 23 6.0 24 9.0 25 12.0 26 14.0 27 16.0 28 28.0 29 40.0 30 49.0 31 58.0 32 68.0 33 78.0 34 88.5 35 99.0 36 133.5 37 168.0 38 191.5 39 228.0 40 241.0 41 270.0 42 299.0 43 299.5 44 300.0 45 324.0 46 348.0 47 332.5 48 317.0 49 320.5 50 324.0 51 333.0 52 342.0 53 334.0 54 326.0 55 273.5 56 221.0 57 201.0 58 181.0 59 167.5 60 154.0 61 129.5 62 105.0 63 80.5 64 45.5 65 35.0 66 26.0 67 17.0 68 13.0 69 9.0 70 7.5 71 6.0 72 5.0 73 4.0 74 4.0 75 4.0 76 2.5 77 1.0 78 1.5 79 2.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.025 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.55 #Duplication Level Percentage of deduplicated Percentage of total 1 93.36533032185206 82.675 2 4.376058723884811 7.75 3 1.0163749294184077 2.7 4 0.36702428006775834 1.3 5 0.3105590062111801 1.375 6 0.16939582156973462 0.8999999999999999 7 0.2258610954263128 1.4000000000000001 8 0.0 0.0 9 0.0564652738565782 0.44999999999999996 >10 0.1129305477131564 1.4500000000000002 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGC 19 0.475 No Hit CTCGTATTTTCCCCTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTG 17 0.42500000000000004 No Hit CCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACT 12 0.3 No Hit CTCCAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACT 10 0.25 No Hit CTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAACGGCA 9 0.22499999999999998 No Hit GGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGA 9 0.22499999999999998 No Hit GTCCCTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAAC 7 0.17500000000000002 No Hit CTTAAGAATGCTGGTTTTAAGAATGAGTGATTGCCCTTCTCCGACCCTTACT 7 0.17500000000000002 No Hit CGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTG 7 0.17500000000000002 No Hit CCCTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAACGG 7 0.17500000000000002 No Hit TGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGTAC 7 0.17500000000000002 No Hit GTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGATG 7 0.17500000000000002 No Hit GGGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCG 7 0.17500000000000002 No Hit ATCAAACTAGAGGTTACCAAAGAACCATGCATAGCACTGAATAGGGAGCCGC 7 0.17500000000000002 No Hit CTCACGAGCAAGATCACGTCCCTCATTACGAGCTTGTACACATGCTTCTAGA 6 0.15 No Hit CCACTTTTTGGTCTTAAGAATGCTGGTTTTAAGAATGAGTGATTGCCCTTCT 6 0.15 No Hit CTTATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCATGTA 6 0.15 No Hit CCTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTGGCCCGCAGCAGT 6 0.15 No Hit GTGAAGATACGTTGTTAGGTGCTCCATTTTATTTTCCCATTGAGGCCGAACC 6 0.15 No Hit CTCAGCCTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGAGGC 6 0.15 No Hit CGCAGAAGTAGGAATAATGGCACCAGAAATGATATTGTTTCCATAAAGTAGA 5 0.125 No Hit CCCCTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTGGCCCGCAGCA 5 0.125 No Hit CTCCATTTTATTTTCCCATTGAGGCCGAACCTAAACCTGTGCTCGAGAGATA 5 0.125 No Hit GGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGT 5 0.125 No Hit GGGTAAACCACCGCCTCTCGGGCCCCCGACTGATTCTACCATAGAGGCCGAC 5 0.125 No Hit GTCCCTTATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCA 5 0.125 No Hit CCAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACTGG 5 0.125 No Hit CTGAGTTGGAATCCCATTCTAACTAAGGATTCTTGTGGTTCCGGAGGATCCA 5 0.125 No Hit GTCCTAGAAGTGCATGATAAATACCGCCAAAGCCCAATACTGCAGAGGAAAT 5 0.125 No Hit CACTTTTTGGTCTTAAGAATGCTGGTTTTAAGAATGAGTGATTGCCCTTCTC 5 0.125 No Hit AGCACGGTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra Read 200000 spots for SRR5423412.sra Written 200000 spots for SRR5423412.sra SRR ids: ['SRR5423412.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_zidvb138 SRR5423412.sra spots: 4000000 blocks: [[1, 200000], [200001, 400000], [400001, 600000], [600001, 800000], [800001, 1000000], [1000001, 1200000], [1200001, 1400000], [1400001, 1600000], [1600001, 1800000], [1800001, 2000000], [2000001, 2200000], [2200001, 2400000], [2400001, 2600000], [2600001, 2800000], [2800001, 3000000], [3000001, 3200000], [3200001, 3400000], [3400001, 3600000], [3600001, 3800000], [3800001, 4000000]] SRR5423412 file size 703954 SRR5423412 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423412 SRR5423412_1.fastq Input file: SRR5423412_1.fastq trimmed: SRR5423412-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Thu Feb 13 07:55:07 2025 >> started Thu Feb 13 08:05:05 2025 >> done (598.424s) 4000000 reads processed; of these: 136 ( 0.00%) short reads filtered out after trimming by size control 58 ( 0.00%) empty reads filtered out after trimming by size control 3999806 (100.00%) reads available; of these: 102448 ( 2.56%) trimmed reads available after processing 3897358 (97.44%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 7 0.00% 19 2 0.00% 20 4 0.00% 21 1 0.00% 22 1 0.00% 23 4 0.00% 24 1 0.00% 25 5 0.00% 26 5 0.00% 27 4 0.00% 28 6 0.00% 29 7 0.00% 30 6 0.00% 31 9 0.00% 32 9 0.00% 33 11 0.00% 34 14 0.00% 35 16 0.00% 36 21 0.00% 37 42 0.00% 38 28 0.00% 39 45 0.00% 40 72 0.00% 41 90 0.00% 42 155 0.00% 43 192 0.00% 44 488 0.01% 45 673 0.02% 46 675 0.02% 47 1043 0.03% 48 1798 0.04% 49 4243 0.11% 50 12246 0.31% 51 80525 2.01% 52 3897358 97.44% 3999806 reads passed initial QC criterion=sequence-density sequence-density=0.48 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=23 prefix-density=0.49 prefix-fanout=1.9 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=144.32 fanout-score-rank=1 prefix-density=0.71 prefix-fanout=1.1 sequence=CCCTCTGCCACAAATTTTGACGTAGGCTCTCCCTCTTGGGGAGACCTTAG Started job on | Feb 13 08:15:48 Started mapping on | Feb 13 08:16:36 Finished on | Feb 13 09:04:42 Mapping speed, Million of reads per hour | 4.99 Number of input reads | 3999806 Average input read length | 51 UNIQUE READS: Uniquely mapped reads number | 3114260 Uniquely mapped reads % | 77.86% Average mapped length | 51.79 Number of splices: Total | 275193 Number of splices: Annotated (sjdb) | 271522 Number of splices: GT/AG | 267372 Number of splices: GC/AG | 6201 Number of splices: AT/AC | 1093 Number of splices: Non-canonical | 527 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.01% Deletion average length | 2.76 Insertion rate per base | 0.00% Insertion average length | 1.34 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 768293 % of reads mapped to multiple loci | 19.21% Number of reads mapped to too many loci | 53571 % of reads mapped to too many loci | 1.34% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.58% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 117253 117253 117253 N_multimapping 768293 768293 768293 N_noFeature 402460 3073723 431524 N_ambiguous 22766 182 11129 UnstrandedReadsAssigned:2689034 PositiveStrandReadsAssigned:40355 NegativeStrandReadsAssigned:2671607 Dataset is classified negative stranded MeadianReadLen=52 20thPercentileLength=52 echo kmer=47 SRR5423412 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR5423412-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,999,806 reads, 3,276,716 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,050 rounds 52401 SRR5423412.ke.tsv 34699 SRR5423412.se.tsv 87100 total ==> SRR5423412.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 84 11.8289 Potri.005G024800.1.v4.1 1035 936 1 0.288712 Potri.004G059700.1.v4.1 961 862 4 1.25399 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 75.6808 7.19113 Potri.016G087400.1.v4.1 270 171 17 26.8654 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 0 0 Potri.012G127500.1.v4.1 977 878 4 1.23114 ==> SRR5423412.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 3 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 17 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 7 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR5423412 completed mapping pipeline successfully