Starting /dee2/code/volunteer_pipeline.sh SRR5423414 current disk space = 2824067125248 free memory = 1581297436 SRR5423414 SRAfilesize 1a0760d715032d3ee371ad3534610b4e SRR5423414.sra SRR5423414.sra file validated SRR5423414 is single end SRR5423414 is conventional basespace SRR5423414 read1 length is 52 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5423414_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3 34.0 31.0 34.0 30.0 34.0 2 32.4745 34.0 31.0 34.0 30.0 34.0 3 32.55525 34.0 31.0 34.0 31.0 34.0 4 35.92225 37.0 35.0 37.0 35.0 37.0 5 35.94525 37.0 35.0 37.0 35.0 37.0 6 35.92325 37.0 35.0 37.0 35.0 37.0 7 35.9095 37.0 35.0 37.0 35.0 37.0 8 35.9905 37.0 35.0 37.0 35.0 37.0 9 37.6915 39.0 37.0 39.0 35.0 39.0 10 37.53275 39.0 37.0 39.0 35.0 39.0 11 37.61975 39.0 37.0 39.0 35.0 39.0 12 37.6745 39.0 37.0 39.0 35.0 39.0 13 37.66475 39.0 37.0 39.0 35.0 39.0 14 39.11775 40.0 38.0 41.0 36.0 41.0 15 38.971 40.0 38.0 41.0 36.0 41.0 16 38.88775 40.0 38.0 41.0 35.0 41.0 17 38.9275 40.0 38.0 41.0 35.0 41.0 18 38.9025 40.0 38.0 41.0 36.0 41.0 19 38.91225 40.0 38.0 41.0 35.0 41.0 20 38.85575 40.0 38.0 41.0 35.0 41.0 21 38.92975 40.0 38.0 41.0 35.0 41.0 22 38.844 40.0 38.0 41.0 35.0 41.0 23 38.7835 40.0 38.0 41.0 34.0 41.0 24 38.88625 40.0 38.0 41.0 35.0 41.0 25 38.773 40.0 38.0 41.0 35.0 41.0 26 38.66075 40.0 38.0 41.0 34.0 41.0 27 38.5035 40.0 38.0 41.0 34.0 41.0 28 38.54275 40.0 38.0 41.0 34.0 41.0 29 38.36025 40.0 38.0 41.0 34.0 41.0 30 38.4915 40.0 38.0 41.0 34.0 41.0 31 38.3425 40.0 38.0 41.0 34.0 41.0 32 38.36625 40.0 38.0 41.0 34.0 41.0 33 38.3175 40.0 38.0 41.0 33.0 41.0 34 38.33175 40.0 38.0 41.0 34.0 41.0 35 38.1025 40.0 38.0 41.0 33.0 41.0 36 38.23275 40.0 38.0 41.0 33.0 41.0 37 38.135 40.0 38.0 41.0 33.0 41.0 38 37.95 40.0 38.0 41.0 33.0 41.0 39 37.93225 40.0 38.0 41.0 33.0 41.0 40 37.77975 40.0 37.0 41.0 33.0 41.0 41 37.822 40.0 38.0 41.0 32.0 41.0 42 37.86075 40.0 38.0 41.0 33.0 41.0 43 37.8075 40.0 37.0 41.0 33.0 41.0 44 37.58525 40.0 37.0 41.0 31.0 41.0 45 37.59325 40.0 37.0 41.0 32.0 41.0 46 37.44225 40.0 37.0 41.0 32.0 41.0 47 37.26625 40.0 37.0 41.0 31.0 41.0 48 37.34825 40.0 37.0 41.0 31.0 41.0 49 37.316 40.0 36.0 41.0 31.0 41.0 50 37.04875 40.0 36.0 41.0 31.0 41.0 51 37.0875 39.0 36.0 41.0 31.0 41.0 52 35.2445 38.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2205 1 0.0 2205 2 0.0 2205 3 0.0 2205 4 0.0 2205 5 0.0 2205 6 0.0 2205 7 0.0 2205 8 0.0 2205 9 0.0 2205 10 0.0 2205 11 0.0 2205 12 0.0 2205 13 0.0 2205 14 0.0 2205 15 0.0 2205 16 0.0 2205 17 0.0 2205 18 0.0 2205 19 0.0 2205 20 0.0 2205 21 0.0 2205 22 0.0 2205 23 0.0 2205 24 0.0 2205 25 0.0 2205 26 0.0 2205 27 0.0 2205 28 0.0 2205 29 0.0 2205 30 0.0 2205 31 0.0 2205 32 0.0 2205 33 0.0 2205 34 0.0 2205 35 0.0 2205 36 0.0 2205 37 0.0 2205 38 0.0 2205 39 0.0 2205 40 0.0 2205 41 0.0 2205 42 0.0 2205 43 0.0 2205 44 0.0 2205 45 0.0 2205 46 0.0 2205 47 0.0 2205 48 0.0 2205 49 0.0 2205 50 0.0 2205 51 0.0 2205 52 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 1.0 22 5.0 23 2.0 24 5.0 25 11.0 26 18.0 27 20.0 28 31.0 29 35.0 30 47.0 31 75.0 32 101.0 33 99.0 34 158.0 35 199.0 36 278.0 37 396.0 38 730.0 39 1785.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.936170212765955 9.762202753441802 6.282853566958699 55.01877346683354 2 21.95 13.25 38.324999999999996 26.474999999999998 3 21.7 16.3 23.175 38.824999999999996 4 26.424999999999997 25.5 18.525 29.549999999999997 5 23.974999999999998 30.65 25.0 20.375 6 19.650000000000002 33.6 24.65 22.1 7 15.525 24.775 39.925 19.775000000000002 8 19.625 22.325 31.3 26.75 9 18.675 20.925 34.8 25.6 10 17.25 37.85 25.25 19.650000000000002 11 23.575 28.675 20.625 27.125 12 21.55 24.3 25.924999999999997 28.225 13 19.8 28.050000000000004 27.175 24.975 14 19.325 30.025000000000002 26.650000000000002 24.0 15 21.325 27.400000000000002 27.175 24.099999999999998 16 20.7 28.225 25.45 25.624999999999996 17 21.975 27.075 26.974999999999998 23.974999999999998 18 20.325 28.4 25.650000000000002 25.624999999999996 19 21.2 28.625 25.324999999999996 24.85 20 20.65 27.525 26.8 25.025 21 20.4 27.275 25.650000000000002 26.674999999999997 22 20.1 28.475 25.55 25.874999999999996 23 23.1 27.925 24.775 24.2 24 20.9 26.75 25.8 26.55 25 20.974999999999998 26.85 26.525 25.650000000000002 26 21.0 27.575 27.900000000000002 23.525 27 21.5 26.6 26.8 25.1 28 21.3 27.700000000000003 25.924999999999997 25.074999999999996 29 21.0 28.599999999999998 26.625 23.775 30 20.325 25.650000000000002 26.25 27.775 31 20.275000000000002 27.875 27.55 24.3 32 21.3 27.325 26.1 25.275 33 21.425 24.375 28.125 26.075 34 20.775 27.525 25.650000000000002 26.05 35 21.175 27.85 24.875 26.1 36 20.825 26.674999999999997 25.174999999999997 27.325 37 21.125 26.6 25.275 27.0 38 21.2 27.650000000000002 25.174999999999997 25.974999999999998 39 20.875 26.375 25.35 27.400000000000002 40 21.5 26.900000000000002 25.924999999999997 25.674999999999997 41 21.8 26.75 26.0 25.45 42 20.525 25.3 27.025 27.150000000000002 43 21.4 27.525 25.174999999999997 25.900000000000002 44 23.400000000000002 27.325 25.825 23.45 45 24.224999999999998 25.174999999999997 24.925 25.674999999999997 46 22.900000000000002 26.75 23.549999999999997 26.8 47 22.45 28.199999999999996 25.474999999999998 23.875 48 22.725 27.224999999999998 23.375 26.674999999999997 49 22.1 25.0 24.825 28.075 50 22.375 27.900000000000002 25.05 24.675 51 22.975 25.474999999999998 24.675 26.875 52 21.875 27.675 24.9 25.55 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 1.0 3 0.5 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.0 10 1.0 11 0.5 12 0.0 13 1.0 14 1.0 15 0.0 16 1.5 17 3.0 18 5.5 19 8.0 20 10.5 21 13.0 22 7.5 23 2.0 24 8.0 25 14.0 26 15.5 27 17.0 28 26.0 29 35.0 30 42.0 31 49.0 32 68.0 33 87.0 34 86.5 35 86.0 36 120.5 37 155.0 38 185.5 39 227.5 40 239.0 41 245.5 42 252.0 43 286.0 44 320.0 45 326.0 46 332.0 47 334.0 48 336.0 49 343.5 50 351.0 51 363.5 52 376.0 53 353.0 54 330.0 55 278.5 56 227.0 57 203.0 58 179.0 59 155.5 60 132.0 61 110.5 62 89.0 63 72.0 64 48.0 65 41.0 66 31.5 67 22.0 68 17.0 69 12.0 70 10.5 71 9.0 72 6.0 73 3.0 74 2.5 75 2.0 76 1.0 77 0.0 78 1.0 79 2.0 80 1.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.125 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.425 #Duplication Level Percentage of deduplicated Percentage of total 1 93.36574206462683 81.625 2 3.86045181584215 6.75 3 1.315413211323992 3.45 4 0.5147269087789533 1.7999999999999998 5 0.3431512725193023 1.5 6 0.14297969688304263 0.75 7 0.057191878753217046 0.35000000000000003 8 0.20017157563625965 1.4000000000000001 9 0.057191878753217046 0.44999999999999996 >10 0.14297969688304263 1.925 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGC 19 0.475 No Hit CTCGTATTTTCCCCTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTG 18 0.44999999999999996 No Hit CCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACT 14 0.35000000000000003 No Hit CTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAACGGCA 13 0.325 No Hit GGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGA 13 0.325 No Hit CGCAGAAGTAGGAATAATGGCACCAGAAATGATATTGTTTCCATAAAGTAGA 9 0.22499999999999998 No Hit CTTATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCATGTA 9 0.22499999999999998 No Hit CCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCCCT 8 0.2 No Hit GTGCTGAGTTGGAATCCCATTCTAACTAAGGATTCTTGTGGTTCCGGAGGAT 8 0.2 No Hit CCTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAACGGC 8 0.2 No Hit GTCCCTTATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCA 8 0.2 No Hit GGGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCG 8 0.2 No Hit CTCCAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACT 8 0.2 No Hit ATCAAACTAGAGGTTACCAAAGAACCATGCATAGCACTGAATAGGGAGCCGC 8 0.2 No Hit CTCACGAGCAAGATCACGTCCCTCATTACGAGCTTGTACACATGCTTCTAGA 7 0.17500000000000002 No Hit CTCGTACACACTAAGAAAAAAGCCTTATCCATTTACAAAAGTCTTATCCATT 7 0.17500000000000002 No Hit GCCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTAC 6 0.15 No Hit GTCCGCGACCCCTGGATGCCGAAGGCGTCCTTGGGGCGATCTCGTAGTTCCT 6 0.15 No Hit CCCGAACACAGCTTACAACTTTCATCGTACTGTGCTCTCCAAAGAGCAACTC 6 0.15 No Hit CTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGAGGCATCCCA 6 0.15 No Hit CCGCCGAATACACCAGCTACGCCTAACATGTGAAATGGGTGCATAAGGATGT 6 0.15 No Hit GTCCCTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAAC 5 0.125 No Hit AACCAGTCCATAAATTGTTAAAGCTTCCATAAAAGCCAGACTAAGCAATAAA 5 0.125 No Hit CCACTTTTTGGTCTTAAGAATGCTGGTTTTAAGAATGAGTGATTGCCCTTCT 5 0.125 No Hit CTCTTGACCAAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTTTCC 5 0.125 No Hit GTCGAATCCAATGATACGGATAAAGGAGTTAGGGTAAGCTTTCTTCGCCTCC 5 0.125 No Hit GTTCTATGGTCGGTCCGCGACCCCTGGATGCCGAAGGCGTCCTTGGGGCGAT 5 0.125 No Hit CCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTG 5 0.125 No Hit CCTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTGGCCCGCAGCAGT 5 0.125 No Hit GTAGAAGATTCAGCAGCTACTGCGGCCCCTGCTTCCTCGGGCGGAACTCCAG 5 0.125 No Hit CCGCCGACTCCAACTATCGTCCATGTACGATCCATACTAGATCTGACCAACT 5 0.125 No Hit GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGTA 5 0.125 No Hit CTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTGGCCCGCAGCAGTA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra Read 200000 spots for SRR5423414.sra Written 200000 spots for SRR5423414.sra SRR ids: ['SRR5423414.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_pr5qyf5j SRR5423414.sra spots: 4000000 blocks: [[1, 200000], [200001, 400000], [400001, 600000], [600001, 800000], [800001, 1000000], [1000001, 1200000], [1200001, 1400000], [1400001, 1600000], [1600001, 1800000], [1800001, 2000000], [2000001, 2200000], [2200001, 2400000], [2400001, 2600000], [2600001, 2800000], [2800001, 3000000], [3000001, 3200000], [3200001, 3400000], [3400001, 3600000], [3600001, 3800000], [3800001, 4000000]] SRR5423414 file size 703948 SRR5423414 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423414 SRR5423414_1.fastq Input file: SRR5423414_1.fastq trimmed: SRR5423414-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Thu Apr 10 12:33:35 2025 >> started Thu Apr 10 12:33:37 2025 >> done (1.859s) 4000000 reads processed; of these: 128 ( 0.00%) short reads filtered out after trimming by size control 49 ( 0.00%) empty reads filtered out after trimming by size control 3999823 (100.00%) reads available; of these: 83342 ( 2.08%) trimmed reads available after processing 3916481 (97.92%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 7 0.00% 19 5 0.00% 20 6 0.00% 21 0 0.00% 22 0 0.00% 23 1 0.00% 24 2 0.00% 25 1 0.00% 26 3 0.00% 27 2 0.00% 28 5 0.00% 29 4 0.00% 30 7 0.00% 31 8 0.00% 32 4 0.00% 33 8 0.00% 34 12 0.00% 35 16 0.00% 36 15 0.00% 37 23 0.00% 38 34 0.00% 39 37 0.00% 40 44 0.00% 41 76 0.00% 42 112 0.00% 43 152 0.00% 44 419 0.01% 45 530 0.01% 46 551 0.01% 47 723 0.02% 48 1413 0.04% 49 3406 0.09% 50 9805 0.25% 51 65911 1.65% 52 3916481 97.92% 3999823 reads passed initial QC criterion=sequence-density sequence-density=0.50 sequence-density-rank=1 fanout-score=1.96 fanout-score-rank=27 prefix-density=0.51 prefix-fanout=1.9 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=130.68 fanout-score-rank=1 prefix-density=0.73 prefix-fanout=1.1 sequence=CCCTCTGCCACAAATTTTGACGTAGGCTCTCCCTCTTGGGGAGACCTTAG Started job on | Apr 10 12:33:54 Started mapping on | Apr 10 12:33:54 Finished on | Apr 10 12:33:59 Mapping speed, Million of reads per hour | 2879.87 Number of input reads | 3999823 Average input read length | 51 UNIQUE READS: Uniquely mapped reads number | 3115222 Uniquely mapped reads % | 77.88% Average mapped length | 51.80 Number of splices: Total | 275956 Number of splices: Annotated (sjdb) | 272553 Number of splices: GT/AG | 268223 Number of splices: GC/AG | 6190 Number of splices: AT/AC | 1074 Number of splices: Non-canonical | 469 Mismatch rate per base, % | 0.36% Deletion rate per base | 0.01% Deletion average length | 2.72 Insertion rate per base | 0.00% Insertion average length | 1.36 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 766453 % of reads mapped to multiple loci | 19.16% Number of reads mapped to too many loci | 55185 % of reads mapped to too many loci | 1.38% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.56% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 118148 118148 118148 N_multimapping 766453 766453 766453 N_noFeature 404089 3074250 433569 N_ambiguous 22645 161 11001 UnstrandedReadsAssigned:2688488 PositiveStrandReadsAssigned:40811 NegativeStrandReadsAssigned:2670652 Dataset is classified negative stranded MeadianReadLen=52 20thPercentileLength=52 echo kmer=47 SRR5423414 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR5423414-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,999,823 reads, 3,281,861 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,099 rounds 52401 SRR5423414.ke.tsv 34699 SRR5423414.se.tsv 87100 total ==> SRR5423414.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 96 13.4751 Potri.005G024800.1.v4.1 1035 936 5 1.4389 Potri.004G059700.1.v4.1 961 862 0 0 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 65.8575 6.23753 Potri.016G087400.1.v4.1 270 171 11 17.3274 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 1 0.160909 Potri.012G127500.1.v4.1 977 878 6 1.84075 ==> SRR5423414.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 2 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 26 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 8 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1 SRR5423414 completed mapping pipeline successfully