Starting /dee2/code/volunteer_pipeline.sh SRR5423424 current disk space = 3050271092736 free memory = 1574476504 SRR5423424 SRAfilesize 9591a06176b9ce11b85eb69c43a47fa4 SRR5423424.sra SRR5423424.sra file validated SRR5423424 is single end SRR5423424 is conventional basespace SRR5423424 read1 length is 52 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5423424_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.59875 34.0 31.0 34.0 31.0 34.0 2 32.706 34.0 31.0 34.0 31.0 34.0 3 32.76275 34.0 31.0 34.0 31.0 34.0 4 36.1205 37.0 37.0 37.0 35.0 37.0 5 36.09175 37.0 37.0 37.0 35.0 37.0 6 36.0995 37.0 37.0 37.0 35.0 37.0 7 36.156 37.0 36.0 37.0 35.0 37.0 8 36.002 37.0 35.0 37.0 35.0 37.0 9 37.787 39.0 38.0 39.0 35.0 39.0 10 37.81925 39.0 38.0 39.0 35.0 39.0 11 37.75925 39.0 38.0 39.0 35.0 39.0 12 37.78175 39.0 38.0 39.0 35.0 39.0 13 37.6765 39.0 37.0 39.0 35.0 39.0 14 39.18225 40.0 39.0 41.0 36.0 41.0 15 39.11475 40.0 38.0 41.0 36.0 41.0 16 39.143 40.0 39.0 41.0 36.0 41.0 17 39.149 40.0 39.0 41.0 36.0 41.0 18 39.0395 40.0 38.0 41.0 36.0 41.0 19 39.112 40.0 39.0 41.0 36.0 41.0 20 39.05875 40.0 39.0 41.0 36.0 41.0 21 39.03825 40.0 39.0 41.0 36.0 41.0 22 39.0725 40.0 39.0 41.0 36.0 41.0 23 38.93775 40.0 39.0 41.0 35.0 41.0 24 38.97275 40.0 39.0 41.0 35.0 41.0 25 38.971 40.0 39.0 41.0 36.0 41.0 26 38.687 40.0 38.0 41.0 34.0 41.0 27 38.80375 40.0 38.0 41.0 35.0 41.0 28 38.74625 40.0 38.0 41.0 35.0 41.0 29 38.7805 40.0 39.0 41.0 35.0 41.0 30 38.781 40.0 38.0 41.0 35.0 41.0 31 38.686 40.0 38.0 41.0 35.0 41.0 32 38.735 40.0 39.0 41.0 35.0 41.0 33 38.57725 40.0 38.0 41.0 34.0 41.0 34 38.46325 40.0 38.0 41.0 34.0 41.0 35 38.20625 40.0 38.0 41.0 33.0 41.0 36 38.2385 40.0 38.0 41.0 33.0 41.0 37 38.162 40.0 38.0 41.0 33.0 41.0 38 38.08375 40.0 38.0 41.0 33.0 41.0 39 38.002 40.0 38.0 41.0 33.0 41.0 40 38.00525 40.0 38.0 41.0 33.0 41.0 41 37.97375 40.0 38.0 41.0 33.0 41.0 42 37.94825 40.0 38.0 41.0 33.0 41.0 43 37.84625 40.0 38.0 41.0 33.0 41.0 44 37.59875 40.0 37.0 41.0 32.0 41.0 45 37.76475 40.0 37.0 41.0 32.0 41.0 46 37.693 40.0 37.0 41.0 32.0 41.0 47 37.3655 40.0 37.0 41.0 31.0 41.0 48 37.3205 40.0 37.0 41.0 31.0 41.0 49 37.29675 40.0 37.0 41.0 31.0 41.0 50 37.3125 40.0 37.0 41.0 31.0 41.0 51 37.21925 40.0 36.0 41.0 31.0 41.0 52 35.56625 38.0 34.0 40.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2202 1 0.0 2202 2 0.0 2202 3 0.0 2202 4 0.0 2202 5 0.0 2202 6 0.0 2202 7 0.0 2202 8 0.0 2202 9 0.0 2202 10 0.0 2202 11 0.0 2202 12 0.0 2202 13 0.0 2202 14 0.0 2202 15 0.0 2202 16 0.0 2202 17 0.0 2202 18 0.0 2202 19 0.0 2202 20 0.0 2202 21 0.0 2202 22 0.0 2202 23 0.0 2202 24 0.0 2202 25 0.0 2202 26 0.0 2202 27 0.0 2202 28 0.0 2202 29 0.0 2202 30 0.0 2202 31 0.0 2202 32 0.0 2202 33 0.0 2202 34 0.0 2202 35 0.0 2202 36 0.0 2202 37 0.0 2202 38 0.0 2202 39 0.0 2202 40 0.0 2202 41 0.0 2202 42 0.0 2202 43 0.0 2202 44 0.0 2202 45 0.0 2202 46 0.0 2202 47 0.0 2202 48 0.0 2202 49 0.0 2202 50 0.0 2202 51 0.0 2202 52 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 0.0 13 0.0 14 1.0 15 0.0 16 1.0 17 1.0 18 0.0 19 1.0 20 4.0 21 2.0 22 6.0 23 4.0 24 8.0 25 9.0 26 15.0 27 20.0 28 24.0 29 39.0 30 49.0 31 53.0 32 88.0 33 95.0 34 121.0 35 152.0 36 253.0 37 357.0 38 720.0 39 1971.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.169084542271136 10.205102551275639 5.7528764382191095 45.872936468234116 2 24.175 13.750000000000002 35.425000000000004 26.650000000000002 3 21.099999999999998 17.150000000000002 22.625 39.125 4 25.3 26.775 21.125 26.8 5 23.9 31.55 23.875 20.674999999999997 6 18.775 33.525 24.55 23.150000000000002 7 16.25 22.8 41.675000000000004 19.275000000000002 8 18.75 23.075000000000003 29.625 28.549999999999997 9 18.475 21.2 33.725 26.6 10 18.175 38.475 24.325 19.025 11 23.325000000000003 28.675 21.8 26.200000000000003 12 21.425 24.5 27.075 27.0 13 19.375 27.825 28.849999999999998 23.95 14 20.3 28.1 27.800000000000004 23.799999999999997 15 20.825 26.625 27.625 24.925 16 20.45 27.025 28.15 24.375 17 22.400000000000002 27.800000000000004 25.4 24.4 18 20.724999999999998 26.400000000000002 27.6 25.275 19 20.875 27.325 26.5 25.3 20 21.575 27.375 26.625 24.425 21 21.15 25.874999999999996 26.5 26.474999999999998 22 21.025 27.250000000000004 25.7 26.025 23 21.025 28.9 25.575 24.5 24 21.775 27.900000000000002 26.400000000000002 23.925 25 21.95 26.025 25.724999999999998 26.3 26 21.65 27.525 25.75 25.074999999999996 27 21.2 28.575 26.575 23.65 28 21.775 27.05 27.975 23.200000000000003 29 21.525 26.674999999999997 27.250000000000004 24.55 30 20.3 24.474999999999998 27.725 27.500000000000004 31 20.75 28.825 25.8 24.625 32 20.925 27.825 26.1 25.15 33 21.4 25.224999999999998 28.1 25.275 34 20.849999999999998 26.974999999999998 26.525 25.650000000000002 35 20.325 27.625 25.575 26.474999999999998 36 21.325 26.125 26.200000000000003 26.35 37 22.025 24.875 27.200000000000003 25.900000000000002 38 21.05 26.400000000000002 25.924999999999997 26.625 39 20.599999999999998 26.275 25.224999999999998 27.900000000000002 40 20.424999999999997 26.6 26.525 26.450000000000003 41 22.400000000000002 26.0 25.8 25.8 42 20.775 25.5 26.775 26.950000000000003 43 21.25 28.499999999999996 25.55 24.7 44 22.025 26.35 26.974999999999998 24.65 45 22.325 24.375 26.0 27.3 46 22.15 26.474999999999998 25.775 25.6 47 22.75 27.075 25.324999999999996 24.85 48 22.136068034017008 26.263131565782892 25.26263131565783 26.338169084542272 49 21.9 25.275 24.75 28.075 50 21.45536384096024 26.85671417854464 25.681420355088775 26.006501625406354 51 22.761380690345174 25.48774387193597 25.812906453226613 25.937968984492244 52 21.9 26.075 25.4 26.625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 1.0 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.5 17 1.0 18 2.5 19 4.0 20 5.5 21 7.0 22 6.0 23 5.0 24 9.0 25 13.0 26 18.5 27 24.0 28 28.0 29 32.0 30 42.5 31 53.0 32 69.0 33 85.0 34 99.0 35 113.0 36 142.0 37 171.0 38 202.0 39 243.0 40 253.0 41 265.0 42 277.0 43 304.0 44 331.0 45 314.0 46 297.0 47 301.0 48 305.0 49 308.5 50 312.0 51 329.0 52 346.0 53 343.0 54 340.0 55 280.0 56 220.0 57 204.0 58 188.0 59 167.5 60 147.0 61 122.5 62 98.0 63 75.0 64 44.0 65 36.0 66 30.5 67 25.0 68 18.5 69 12.0 70 8.5 71 5.0 72 5.5 73 6.0 74 4.5 75 3.0 76 1.5 77 0.0 78 0.5 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 1.5 92 3.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.05 49 0.0 50 0.025 51 0.05 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.10000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 94.22863485016649 84.89999999999999 2 3.7458379578246395 6.75 3 0.804661487236404 2.175 4 0.5549389567147613 2.0 5 0.13873473917869034 0.625 6 0.1664816870144284 0.8999999999999999 7 0.1664816870144284 1.05 8 0.11098779134295228 0.8 9 0.0 0.0 >10 0.0832408435072142 0.8 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGA 12 0.3 No Hit GGGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCG 10 0.25 No Hit GTGAAGATACGTTGTTAGGTGCTCCATTTTATTTTCCCATTGAGGCCGAACC 10 0.25 No Hit CGCAGAAGTAGGAATAATGGCACCAGAAATGATATTGTTTCCATAAAGTAGA 8 0.2 No Hit CTCGTATTTTCCCCTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTG 8 0.2 No Hit CCAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACTGG 8 0.2 No Hit CTCGTACACACTAAGAAAAAAGCCTTATCCATTTACAAAAGTCTTATCCATT 8 0.2 No Hit GCCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTAC 7 0.17500000000000002 No Hit GTCCCTTATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCA 7 0.17500000000000002 No Hit CTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGCCC 7 0.17500000000000002 No Hit GCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGCCCGC 7 0.17500000000000002 No Hit CCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACT 7 0.17500000000000002 No Hit ATCAAACTAGAGGTTACCAAAGAACCATGCATAGCACTGAATAGGGAGCCGC 7 0.17500000000000002 No Hit GTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCCCTGCTTCCTCGG 6 0.15 No Hit CCCTGATCAAACTAGAGGTTACCAAAGAACCATGCATAGCACTGAATAGGGA 6 0.15 No Hit CTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGAGGCATCCCA 6 0.15 No Hit GGCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGATC 6 0.15 No Hit GTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCC 6 0.15 No Hit CTCAGCCTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGAGGC 6 0.15 No Hit CTCACGAGCAAGATCACGTCCCTCATTACGAGCTTGTACACATGCTTCTAGA 5 0.125 No Hit CACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCCCTG 5 0.125 No Hit CGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGC 5 0.125 No Hit GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGTA 5 0.125 No Hit GCCAGATACAAAGTATGGAAAGGTGTCTATGACTTCCCCGCCAGGACCCACC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra Read 200000 spots for SRR5423424.sra Written 200000 spots for SRR5423424.sra SRR ids: ['SRR5423424.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_bo6riwvc SRR5423424.sra spots: 4000000 blocks: [[1, 200000], [200001, 400000], [400001, 600000], [600001, 800000], [800001, 1000000], [1000001, 1200000], [1200001, 1400000], [1400001, 1600000], [1600001, 1800000], [1800001, 2000000], [2000001, 2200000], [2200001, 2400000], [2400001, 2600000], [2600001, 2800000], [2800001, 3000000], [3000001, 3200000], [3200001, 3400000], [3400001, 3600000], [3600001, 3800000], [3800001, 4000000]] SRR5423424 file size 703963 SRR5423424 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423424 SRR5423424_1.fastq Input file: SRR5423424_1.fastq trimmed: SRR5423424-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Wed Feb 12 10:06:14 2025 >> started Wed Feb 12 10:06:16 2025 >> done (2.063s) 4000000 reads processed; of these: 168 ( 0.00%) short reads filtered out after trimming by size control 89 ( 0.00%) empty reads filtered out after trimming by size control 3999743 (99.99%) reads available; of these: 77285 ( 1.93%) trimmed reads available after processing 3922458 (98.07%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 11 0.00% 19 3 0.00% 20 5 0.00% 21 1 0.00% 22 0 0.00% 23 1 0.00% 24 4 0.00% 25 2 0.00% 26 2 0.00% 27 5 0.00% 28 2 0.00% 29 5 0.00% 30 6 0.00% 31 12 0.00% 32 8 0.00% 33 7 0.00% 34 20 0.00% 35 18 0.00% 36 27 0.00% 37 23 0.00% 38 41 0.00% 39 57 0.00% 40 74 0.00% 41 94 0.00% 42 134 0.00% 43 162 0.00% 44 324 0.01% 45 338 0.01% 46 557 0.01% 47 781 0.02% 48 1501 0.04% 49 3287 0.08% 50 9825 0.25% 51 59948 1.50% 52 3922458 98.07% 3999743 reads passed initial QC criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=31 prefix-density=0.46 prefix-fanout=2.0 sequence=TGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGAC criterion=fanout-score sequence-density=0.02 sequence-density-rank=28 fanout-score=23.33 fanout-score-rank=1 prefix-density=0.30 prefix-fanout=1.2 sequence=CATTTTATTTCTATCTTTCCATACATAACCAAAAAATGGAAAAGATTCTTCAAGAGTCTCGGGTCCTAGAAGTGCATGATAAATACCGCCAAAGCCCAATACTGCAGAGGAAATTAAGTGAAGTACGCCAGATACAAAGTATGGAAAGGTGTCTATGACTTCCCCGCCAGGACCCACCCCCCAACCTAGAGTAGCTAGGTGGGGAAGTAAAATTAATCCTTGTTCATACATTGGCTTCTCCGGTACGAAATGGGCCACTTCAAATAGGTTCATTGCTCCGGCCCAGAATACGATTAATCCAGCATGGGCTACATGAGCTCCCAGCAG Started job on | Feb 12 10:06:26 Started mapping on | Feb 12 10:06:26 Finished on | Feb 12 10:06:32 Mapping speed, Million of reads per hour | 2399.85 Number of input reads | 3999743 Average input read length | 51 UNIQUE READS: Uniquely mapped reads number | 3137172 Uniquely mapped reads % | 78.43% Average mapped length | 51.79 Number of splices: Total | 289404 Number of splices: Annotated (sjdb) | 284781 Number of splices: GT/AG | 282735 Number of splices: GC/AG | 5372 Number of splices: AT/AC | 789 Number of splices: Non-canonical | 508 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.01% Deletion average length | 2.27 Insertion rate per base | 0.00% Insertion average length | 1.37 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 710778 % of reads mapped to multiple loci | 17.77% Number of reads mapped to too many loci | 82731 % of reads mapped to too many loci | 2.07% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.71% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 151793 151793 151793 N_multimapping 710778 710778 710778 N_noFeature 394751 3091542 429098 N_ambiguous 21956 163 10519 UnstrandedReadsAssigned:2720465 PositiveStrandReadsAssigned:45467 NegativeStrandReadsAssigned:2697555 Dataset is classified negative stranded MeadianReadLen=52 20thPercentileLength=52 echo kmer=47 SRR5423424 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR5423424-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,999,743 reads, 3,287,992 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,132 rounds 52401 SRR5423424.ke.tsv 34699 SRR5423424.se.tsv 87100 total ==> SRR5423424.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 131 19.0904 Potri.005G024800.1.v4.1 1035 936 18 5.37794 Potri.004G059700.1.v4.1 961 862 6 1.94654 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 53.3178 5.24278 Potri.016G087400.1.v4.1 270 171 15 24.5309 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 2 0.334113 Potri.012G127500.1.v4.1 977 878 8 2.54809 ==> SRR5423424.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 2 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 29 Potri.001G212900.v4.1 5 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR5423424 completed mapping pipeline successfully