Starting /dee2/code/volunteer_pipeline.sh SRR5423426 current disk space = 3051093577728 free memory = 1573883408 SRR5423426 SRAfilesize a0174fbf125fee99c343d65077680819 SRR5423426.sra SRR5423426.sra file validated SRR5423426 is single end SRR5423426 is conventional basespace SRR5423426 read1 length is 52 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5423426_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.282 34.0 31.0 34.0 30.0 34.0 2 32.30175 34.0 31.0 34.0 30.0 34.0 3 32.32575 34.0 31.0 34.0 30.0 34.0 4 35.8765 37.0 35.0 37.0 33.0 37.0 5 35.78825 37.0 35.0 37.0 35.0 37.0 6 35.8845 37.0 35.0 37.0 35.0 37.0 7 35.80025 37.0 35.0 37.0 35.0 37.0 8 35.913 37.0 35.0 37.0 35.0 37.0 9 37.654 39.0 37.0 39.0 35.0 39.0 10 37.5675 39.0 37.0 39.0 35.0 39.0 11 37.5845 39.0 37.0 39.0 35.0 39.0 12 37.5355 39.0 37.0 39.0 35.0 39.0 13 37.5745 39.0 37.0 39.0 35.0 39.0 14 38.8985 40.0 38.0 41.0 35.0 41.0 15 38.78475 40.0 38.0 41.0 34.0 41.0 16 38.809 40.0 38.0 41.0 35.0 41.0 17 38.6885 40.0 38.0 41.0 34.0 41.0 18 38.75825 40.0 38.0 41.0 35.0 41.0 19 38.73625 40.0 38.0 41.0 34.0 41.0 20 38.56 40.0 38.0 41.0 34.0 41.0 21 38.689 40.0 38.0 41.0 34.0 41.0 22 38.4625 40.0 38.0 41.0 34.0 41.0 23 38.7295 40.0 38.0 41.0 34.0 41.0 24 38.6405 40.0 38.0 41.0 34.0 41.0 25 38.50925 40.0 38.0 41.0 34.0 41.0 26 38.54375 40.0 38.0 41.0 34.0 41.0 27 38.461 40.0 38.0 41.0 34.0 41.0 28 38.3745 40.0 38.0 41.0 33.0 41.0 29 38.108 40.0 38.0 41.0 33.0 41.0 30 38.1985 40.0 38.0 41.0 33.0 41.0 31 38.2935 40.0 38.0 41.0 34.0 41.0 32 38.19475 40.0 38.0 41.0 33.0 41.0 33 38.3585 40.0 38.0 41.0 34.0 41.0 34 38.30625 40.0 38.0 41.0 34.0 41.0 35 38.3435 40.0 38.0 41.0 34.0 41.0 36 38.22325 40.0 38.0 41.0 33.0 41.0 37 38.16325 40.0 38.0 41.0 33.0 41.0 38 38.08175 40.0 38.0 41.0 33.0 41.0 39 38.21275 40.0 38.0 41.0 33.0 41.0 40 37.987 40.0 38.0 41.0 33.0 41.0 41 38.0225 40.0 37.0 41.0 33.0 41.0 42 37.9565 40.0 37.0 41.0 33.0 41.0 43 37.9355 40.0 37.0 41.0 33.0 41.0 44 37.88275 40.0 37.0 41.0 33.0 41.0 45 37.81475 40.0 37.0 41.0 33.0 41.0 46 37.69375 40.0 37.0 41.0 32.0 41.0 47 37.61775 40.0 37.0 41.0 33.0 41.0 48 37.66375 40.0 37.0 41.0 32.0 41.0 49 37.5785 40.0 37.0 41.0 32.0 41.0 50 37.6095 40.0 37.0 41.0 32.0 41.0 51 37.41825 40.0 36.0 41.0 32.0 41.0 52 36.414 38.0 35.0 40.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2308 1 0.0 2308 2 0.0 2308 3 0.0 2308 4 0.0 2308 5 0.0 2308 6 0.0 2308 7 0.0 2308 8 0.0 2308 9 0.0 2308 10 0.0 2308 11 0.0 2308 12 0.0 2308 13 0.0 2308 14 0.0 2308 15 0.0 2308 16 0.0 2308 17 0.0 2308 18 0.0 2308 19 0.0 2308 20 0.0 2308 21 0.0 2308 22 0.0 2308 23 0.0 2308 24 0.0 2308 25 0.0 2308 26 0.0 2308 27 0.0 2308 28 0.0 2308 29 0.0 2308 30 0.0 2308 31 0.0 2308 32 0.0 2308 33 0.0 2308 34 0.0 2308 35 0.0 2308 36 0.0 2308 37 0.0 2308 38 0.0 2308 39 0.0 2308 40 0.0 2308 41 0.0 2308 42 0.0 2308 43 0.0 2308 44 0.0 2308 45 0.0 2308 46 0.0 2308 47 0.0 2308 48 0.0 2308 49 0.0 2308 50 0.0 2308 51 0.0 2308 52 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 3.0 22 4.0 23 5.0 24 5.0 25 7.0 26 16.0 27 16.0 28 31.0 29 33.0 30 56.0 31 83.0 32 96.0 33 127.0 34 143.0 35 170.0 36 276.0 37 396.0 38 715.0 39 1808.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.17863397548161 11.43357518138604 5.729296972729547 44.65849387040281 2 23.875 14.149999999999999 35.075 26.900000000000002 3 21.475 18.099999999999998 23.549999999999997 36.875 4 26.35 26.150000000000002 19.975 27.525 5 24.5 31.125000000000004 23.974999999999998 20.4 6 20.150000000000002 32.0 23.799999999999997 24.05 7 14.875 23.325000000000003 41.9 19.900000000000002 8 17.825 22.15 31.025000000000002 28.999999999999996 9 18.55 20.65 32.975 27.825 10 19.75 36.55 24.375 19.325 11 22.675 27.1 22.45 27.775 12 19.425 25.275 27.025 28.275 13 19.900000000000002 26.900000000000002 28.875 24.325 14 21.05 28.65 26.724999999999998 23.575 15 20.3 27.750000000000004 26.525 25.424999999999997 16 20.125 27.474999999999998 26.924999999999997 25.474999999999998 17 20.75 27.250000000000004 27.875 24.125 18 20.974999999999998 26.125 27.224999999999998 25.674999999999997 19 20.75 27.275 26.424999999999997 25.55 20 21.95 25.15 26.900000000000002 26.0 21 21.325 25.874999999999996 26.8 26.0 22 19.975 28.625 25.874999999999996 25.525 23 23.025000000000002 26.174999999999997 25.174999999999997 25.624999999999996 24 20.8 25.074999999999996 26.825 27.3 25 20.9 27.250000000000004 26.174999999999997 25.674999999999997 26 22.475 26.450000000000003 26.974999999999998 24.099999999999998 27 21.925 26.125 26.8 25.15 28 21.15 27.325 27.224999999999998 24.3 29 21.224999999999998 28.549999999999997 26.75 23.474999999999998 30 21.425 25.85 25.650000000000002 27.075 31 20.575 29.299999999999997 26.424999999999997 23.7 32 21.325 25.6 27.975 25.1 33 20.65 25.124999999999996 27.700000000000003 26.525 34 21.85 26.35 26.1 25.7 35 20.549999999999997 27.275 25.5 26.674999999999997 36 22.35 25.15 25.575 26.924999999999997 37 20.275000000000002 26.724999999999998 26.525 26.474999999999998 38 22.475 25.85 25.275 26.400000000000002 39 21.224999999999998 26.650000000000002 25.35 26.775 40 21.2 27.025 25.6 26.174999999999997 41 21.675 26.075 26.35 25.900000000000002 42 22.325 25.124999999999996 25.650000000000002 26.900000000000002 43 21.775 26.875 24.875 26.474999999999998 44 21.7 28.125 25.324999999999996 24.85 45 22.075 24.474999999999998 25.95 27.500000000000004 46 22.95 26.724999999999998 24.675 25.650000000000002 47 22.425 28.299999999999997 23.599999999999998 25.674999999999997 48 22.3 27.1 24.625 25.974999999999998 49 21.275 26.5 25.525 26.700000000000003 50 22.25 26.674999999999997 25.1 25.974999999999998 51 21.475 26.575 25.95 26.0 52 22.7 27.150000000000002 24.625 25.525 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.5 2 1.0 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 0.5 12 0.0 13 0.0 14 0.5 15 1.0 16 1.5 17 2.0 18 3.0 19 4.0 20 4.0 21 4.0 22 6.0 23 8.0 24 11.0 25 14.0 26 18.5 27 23.0 28 29.5 29 36.0 30 40.5 31 45.0 32 59.5 33 74.0 34 91.0 35 108.0 36 138.5 37 169.0 38 188.0 39 228.5 40 250.0 41 254.5 42 259.0 43 288.5 44 318.0 45 326.0 46 334.0 47 328.5 48 323.0 49 327.5 50 332.0 51 326.0 52 320.0 53 326.0 54 332.0 55 284.0 56 236.0 57 210.5 58 185.0 59 158.5 60 132.0 61 118.5 62 105.0 63 89.5 64 55.0 65 36.0 66 29.5 67 23.0 68 19.5 69 16.0 70 13.0 71 10.0 72 8.5 73 7.0 74 6.5 75 6.0 76 4.0 77 2.0 78 2.0 79 2.0 80 1.0 81 0.0 82 0.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.375 #Duplication Level Percentage of deduplicated Percentage of total 1 94.04195804195804 84.05 2 3.6923076923076925 6.6000000000000005 3 0.9790209790209791 2.625 4 0.6153846153846154 2.1999999999999997 5 0.22377622377622378 1.0 6 0.08391608391608392 0.44999999999999996 7 0.11188811188811189 0.7000000000000001 8 0.055944055944055944 0.4 9 0.08391608391608392 0.675 >10 0.11188811188811189 1.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGC 16 0.4 No Hit CCAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACTGG 14 0.35000000000000003 No Hit CTCAGCCTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGAGGC 12 0.3 No Hit CAGGGCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAG 10 0.25 No Hit GTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGATG 9 0.22499999999999998 No Hit CCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACT 9 0.22499999999999998 No Hit GGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGA 9 0.22499999999999998 No Hit GGGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCG 8 0.2 No Hit CTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAACGGCA 8 0.2 No Hit GTCCCTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAAC 7 0.17500000000000002 No Hit CTCGTATTTTCCCCTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTG 7 0.17500000000000002 No Hit GTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGC 7 0.17500000000000002 No Hit CCGCCGAATACACCAGCTACGCCTAACATGTGAAATGGGTGCATAAGGATGT 7 0.17500000000000002 No Hit CTCGGGTCCTAGAAGTGCATGATAAATACCGCCAAAGCCCAATACTGCAGAG 6 0.15 No Hit GTCGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAAT 6 0.15 No Hit CAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACTGGT 6 0.15 No Hit GCCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTAC 5 0.125 No Hit GTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCT 5 0.125 No Hit CGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTG 5 0.125 No Hit CTTGACCAAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTTTCCCT 5 0.125 No Hit CTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGCCC 5 0.125 No Hit CTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGAGGCATCCCA 5 0.125 No Hit CTCGTACACACTAAGAAAAAAGCCTTATCCATTTACAAAAGTCTTATCCATT 5 0.125 No Hit AGCACGGTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161690 spots for SRR5423426.sra Written 161690 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra Read 161673 spots for SRR5423426.sra Written 161673 spots for SRR5423426.sra SRR ids: ['SRR5423426.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_czmdcfip SRR5423426.sra spots: 3233477 blocks: [[1, 161673], [161674, 323346], [323347, 485019], [485020, 646692], [646693, 808365], [808366, 970038], [970039, 1131711], [1131712, 1293384], [1293385, 1455057], [1455058, 1616730], [1616731, 1778403], [1778404, 1940076], [1940077, 2101749], [2101750, 2263422], [2263423, 2425095], [2425096, 2586768], [2586769, 2748441], [2748442, 2910114], [2910115, 3071787], [3071788, 3233477]] SRR5423426 file size 568848 SRR5423426 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423426 SRR5423426_1.fastq Input file: SRR5423426_1.fastq trimmed: SRR5423426-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Wed Feb 12 10:38:36 2025 >> started Wed Feb 12 10:38:38 2025 >> done (2.053s) 3233477 reads processed; of these: 111 ( 0.00%) short reads filtered out after trimming by size control 66 ( 0.00%) empty reads filtered out after trimming by size control 3233300 (99.99%) reads available; of these: 50342 ( 1.56%) trimmed reads available after processing 3182958 (98.44%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 12 0.00% 19 3 0.00% 20 4 0.00% 21 0 0.00% 22 0 0.00% 23 0 0.00% 24 0 0.00% 25 0 0.00% 26 0 0.00% 27 0 0.00% 28 0 0.00% 29 0 0.00% 30 1 0.00% 31 2 0.00% 32 3 0.00% 33 6 0.00% 34 7 0.00% 35 9 0.00% 36 15 0.00% 37 16 0.00% 38 10 0.00% 39 16 0.00% 40 32 0.00% 41 27 0.00% 42 49 0.00% 43 76 0.00% 44 114 0.00% 45 148 0.00% 46 234 0.01% 47 358 0.01% 48 709 0.02% 49 1763 0.05% 50 5790 0.18% 51 40938 1.27% 52 3182958 98.44% 3233300 reads passed initial QC criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=29 prefix-density=0.46 prefix-fanout=2.0 sequence=TGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGAC criterion=fanout-score sequence-density=0.02 sequence-density-rank=28 fanout-score=24.88 fanout-score-rank=1 prefix-density=0.31 prefix-fanout=1.2 sequence=CATTTTATTTCTATCTTTCCATACATAACCAAAAAATGGAAAAGATTCTTCAAGAGTCTCGGGTCCTAGAAGTGCATGATAAATACCGCCAAAGCCCAATACTGCAGAGGAAATTAAGTGAAGTACGCCAGATACAAAGTATGGAAAGGTGTCTATGACTTCCCCGCCAGGACCCACCCCCCAACCTAGAGTAGCTAGGTGGGGAAGTAAAATTAATCCTTGTTCATACATTGGCTTCTCCGGTACGAAATGGGCCACTTCAAATAGGTTCATTGCTCCGGCCCAGAATACGATTAATCCAGCATGGGCTACATGAGCTCCCAGCAG Started job on | Feb 12 10:38:51 Started mapping on | Feb 12 10:38:51 Finished on | Feb 12 10:38:58 Mapping speed, Million of reads per hour | 1662.84 Number of input reads | 3233300 Average input read length | 51 UNIQUE READS: Uniquely mapped reads number | 2529921 Uniquely mapped reads % | 78.25% Average mapped length | 51.80 Number of splices: Total | 232287 Number of splices: Annotated (sjdb) | 228613 Number of splices: GT/AG | 226990 Number of splices: GC/AG | 4280 Number of splices: AT/AC | 611 Number of splices: Non-canonical | 406 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.01% Deletion average length | 2.22 Insertion rate per base | 0.00% Insertion average length | 1.36 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 577600 % of reads mapped to multiple loci | 17.86% Number of reads mapped to too many loci | 69793 % of reads mapped to too many loci | 2.16% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.72% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 125779 125779 125779 N_multimapping 577600 577600 577600 N_noFeature 321061 2493285 348639 N_ambiguous 17607 122 8438 UnstrandedReadsAssigned:2191253 PositiveStrandReadsAssigned:36514 NegativeStrandReadsAssigned:2172844 Dataset is classified negative stranded MeadianReadLen=52 20thPercentileLength=52 echo kmer=47 SRR5423426 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR5423426-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,233,300 reads, 2,650,739 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,062 rounds 52401 SRR5423426.ke.tsv 34699 SRR5423426.se.tsv 87100 total ==> SRR5423426.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 100 17.9319 Potri.005G024800.1.v4.1 1035 936 10 3.67642 Potri.004G059700.1.v4.1 961 862 4 1.59681 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 46.3551 5.60879 Potri.016G087400.1.v4.1 270 171 13 26.1606 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 4 0.822253 Potri.012G127500.1.v4.1 977 878 5 1.95964 ==> SRR5423426.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 1 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 45 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 1 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR5423426 completed mapping pipeline successfully