Starting /dee2/code/volunteer_pipeline.sh SRR5423432 current disk space = 3051294371840 free memory = 1573436280 SRR5423432 SRAfilesize 7163ff00e4073c28de84ac979c7f4200 SRR5423432.sra SRR5423432.sra file validated SRR5423432 is single end SRR5423432 is conventional basespace SRR5423432 read1 length is 52 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5423432_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94025 33.0 31.0 34.0 30.0 34.0 2 32.14 34.0 31.0 34.0 30.0 34.0 3 32.2515 34.0 31.0 34.0 30.0 34.0 4 35.67325 37.0 35.0 37.0 33.0 37.0 5 35.648 37.0 35.0 37.0 33.0 37.0 6 35.7185 37.0 35.0 37.0 35.0 37.0 7 35.76625 37.0 35.0 37.0 33.0 37.0 8 35.76525 37.0 35.0 37.0 33.0 37.0 9 37.36 39.0 37.0 39.0 34.0 39.0 10 37.3165 39.0 37.0 39.0 34.0 39.0 11 37.39125 39.0 37.0 39.0 34.0 39.0 12 37.29625 39.0 37.0 39.0 34.0 39.0 13 37.437 39.0 37.0 39.0 35.0 39.0 14 38.676 40.0 38.0 41.0 35.0 41.0 15 38.6125 40.0 38.0 41.0 34.0 41.0 16 38.5 40.0 38.0 41.0 33.0 41.0 17 38.485 40.0 38.0 41.0 34.0 41.0 18 38.5165 40.0 38.0 41.0 34.0 41.0 19 38.59575 40.0 38.0 41.0 34.0 41.0 20 38.5265 40.0 38.0 41.0 34.0 41.0 21 38.35275 40.0 38.0 41.0 33.0 41.0 22 38.289 40.0 38.0 41.0 34.0 41.0 23 38.275 40.0 38.0 41.0 34.0 41.0 24 38.134 40.0 38.0 41.0 33.0 41.0 25 38.40625 40.0 38.0 41.0 34.0 41.0 26 38.27025 40.0 38.0 41.0 34.0 41.0 27 38.09375 40.0 38.0 41.0 33.0 41.0 28 38.32375 40.0 38.0 41.0 34.0 41.0 29 38.03625 40.0 38.0 41.0 33.0 41.0 30 38.04575 40.0 38.0 41.0 33.0 41.0 31 37.98675 40.0 37.0 41.0 33.0 41.0 32 37.758 40.0 37.0 41.0 33.0 41.0 33 37.6965 40.0 37.0 41.0 33.0 41.0 34 37.70125 40.0 37.0 41.0 32.0 41.0 35 37.76725 40.0 37.0 41.0 32.0 41.0 36 37.62225 40.0 37.0 41.0 32.0 41.0 37 37.4405 40.0 37.0 41.0 31.0 41.0 38 37.33225 40.0 37.0 41.0 31.0 41.0 39 37.57725 40.0 37.0 41.0 31.0 41.0 40 37.45575 40.0 37.0 41.0 31.0 41.0 41 37.42325 40.0 37.0 41.0 31.0 41.0 42 37.304 40.0 37.0 41.0 31.0 41.0 43 36.82675 39.0 36.0 41.0 30.0 41.0 44 36.94925 39.0 36.0 41.0 30.0 41.0 45 36.98475 39.0 36.0 41.0 30.0 41.0 46 36.9005 39.0 36.0 41.0 30.0 41.0 47 36.83175 39.0 36.0 41.0 30.0 41.0 48 36.58175 39.0 35.0 41.0 29.0 41.0 49 36.69625 39.0 35.0 41.0 30.0 41.0 50 36.677 39.0 35.0 41.0 30.0 41.0 51 36.57775 39.0 35.0 40.0 30.0 41.0 52 34.55225 38.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2107 1 0.0 2107 2 0.0 2107 3 0.0 2107 4 0.0 2107 5 0.0 2107 6 0.0 2107 7 0.0 2107 8 0.0 2107 9 0.0 2107 10 0.0 2107 11 0.0 2107 12 0.0 2107 13 0.0 2107 14 0.0 2107 15 0.0 2107 16 0.0 2107 17 0.0 2107 18 0.0 2107 19 0.0 2107 20 0.0 2107 21 0.0 2107 22 0.0 2107 23 0.0 2107 24 0.0 2107 25 0.0 2107 26 0.0 2107 27 0.0 2107 28 0.0 2107 29 0.0 2107 30 0.0 2107 31 0.0 2107 32 0.0 2107 33 0.0 2107 34 0.0 2107 35 0.0 2107 36 0.0 2107 37 0.0 2107 38 0.0 2107 39 0.0 2107 40 0.0 2107 41 0.0 2107 42 0.0 2107 43 0.0 2107 44 0.0 2107 45 0.0 2107 46 0.0 2107 47 0.0 2107 48 0.0 2107 49 0.0 2107 50 0.0 2107 51 0.0 2107 52 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 5.0 22 5.0 23 6.0 24 10.0 25 24.0 26 12.0 27 25.0 28 32.0 29 44.0 30 69.0 31 92.0 32 108.0 33 151.0 34 184.0 35 231.0 36 325.0 37 458.0 38 746.0 39 1470.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.7913741223671 11.058174523570711 6.344032096288867 43.80641925777332 2 22.85 15.1 34.9 27.150000000000002 3 21.55 17.474999999999998 22.825 38.15 4 24.65 25.575 21.7 28.075 5 24.6 31.775 21.875 21.75 6 19.15 33.825 23.525 23.5 7 14.549999999999999 23.599999999999998 41.949999999999996 19.900000000000002 8 18.85 22.2 30.0 28.95 9 18.05 20.4 34.425 27.125 10 18.375 38.05 24.275 19.3 11 23.375 28.725 20.575 27.325 12 20.474999999999998 25.224999999999998 26.700000000000003 27.6 13 19.775000000000002 27.275 28.475 24.474999999999998 14 21.525 27.800000000000004 26.875 23.799999999999997 15 21.65 26.25 27.375 24.725 16 21.25 26.974999999999998 26.875 24.9 17 21.975 28.025 25.4 24.6 18 21.425 26.325 26.625 25.624999999999996 19 20.625 27.425 26.375 25.575 20 22.45 27.200000000000003 25.575 24.775 21 19.75 27.025 27.200000000000003 26.025 22 20.625 27.400000000000002 25.624999999999996 26.35 23 21.85 28.199999999999996 25.324999999999996 24.625 24 22.05 26.5 26.200000000000003 25.25 25 21.675 26.6 25.775 25.95 26 21.975 27.200000000000003 25.3 25.525 27 20.75 26.424999999999997 27.500000000000004 25.324999999999996 28 20.95 28.15 26.625 24.275 29 21.85 26.974999999999998 27.275 23.9 30 20.424999999999997 26.0 26.950000000000003 26.625 31 20.0 27.85 27.125 25.025 32 20.875 27.075 27.025 25.025 33 21.025 25.7 26.85 26.424999999999997 34 21.425 27.400000000000002 26.424999999999997 24.75 35 20.150000000000002 26.775 25.85 27.224999999999998 36 20.5 25.900000000000002 25.900000000000002 27.700000000000003 37 22.25 25.825 26.55 25.374999999999996 38 21.575 26.450000000000003 25.55 26.424999999999997 39 21.05 24.9 25.275 28.775000000000002 40 21.05 27.975 25.85 25.124999999999996 41 21.65 27.125 27.05 24.175 42 20.75 25.2 27.025 27.025 43 22.6 26.3 25.3 25.8 44 22.875 27.200000000000003 25.674999999999997 24.25 45 22.5 25.6 25.2 26.700000000000003 46 23.175 25.974999999999998 25.275 25.575 47 22.075 27.875 24.25 25.8 48 22.25 26.05 25.374999999999996 26.325 49 22.575 25.95 24.375 27.1 50 23.075000000000003 26.3 25.324999999999996 25.3 51 21.7 24.425 26.8 27.075 52 23.200000000000003 26.025 25.25 25.525 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.0 16 1.5 17 2.0 18 3.5 19 5.0 20 5.0 21 5.0 22 8.0 23 11.0 24 11.0 25 11.0 26 16.5 27 22.0 28 30.5 29 39.0 30 42.0 31 45.0 32 58.0 33 71.0 34 89.5 35 108.0 36 143.0 37 178.0 38 205.0 39 242.5 40 253.0 41 247.5 42 242.0 43 267.0 44 292.0 45 307.0 46 322.0 47 324.0 48 326.0 49 329.0 50 332.0 51 349.5 52 367.0 53 336.5 54 306.0 55 264.0 56 222.0 57 197.5 58 173.0 59 162.0 60 151.0 61 133.5 62 116.0 63 93.5 64 53.5 65 36.0 66 28.5 67 21.0 68 20.5 69 20.0 70 15.5 71 11.0 72 7.0 73 3.0 74 2.0 75 1.0 76 3.0 77 5.0 78 2.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.375 #Duplication Level Percentage of deduplicated Percentage of total 1 94.12587412587412 84.125 2 3.5244755244755246 6.3 3 1.090909090909091 2.9250000000000003 4 0.44755244755244755 1.6 5 0.3916083916083916 1.7500000000000002 6 0.08391608391608392 0.44999999999999996 7 0.11188811188811189 0.7000000000000001 8 0.055944055944055944 0.4 9 0.08391608391608392 0.675 >10 0.08391608391608392 1.075 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGC 17 0.42500000000000004 No Hit CCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACT 14 0.35000000000000003 No Hit CTCCAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACT 12 0.3 No Hit CTCGTATTTTCCCCTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTG 9 0.22499999999999998 No Hit GGGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCG 9 0.22499999999999998 No Hit GGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGA 9 0.22499999999999998 No Hit CTTATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCATGTA 8 0.2 No Hit CTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGAGGCATCCCA 8 0.2 No Hit GGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCC 7 0.17500000000000002 No Hit CAGGGCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAG 7 0.17500000000000002 No Hit GTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGA 7 0.17500000000000002 No Hit GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGTA 7 0.17500000000000002 No Hit GTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCG 6 0.15 No Hit GCCGCCGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGACGCATACCCA 6 0.15 No Hit CCCGAAGCCACGGGTAGAACACCCGGTAGAGAGACCCAATCTTGAGTGAAAT 6 0.15 No Hit GGGCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGAT 5 0.125 No Hit GTCCCTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAAC 5 0.125 No Hit CTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTTTCCCTGATCAAACTAG 5 0.125 No Hit GCTCAGCCTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGAGG 5 0.125 No Hit GGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGT 5 0.125 No Hit GTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCCCTGCTTCCTCGG 5 0.125 No Hit CGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTG 5 0.125 No Hit CTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAACGGCA 5 0.125 No Hit GCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGCCCGC 5 0.125 No Hit CCTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTGGCCCGCAGCAGT 5 0.125 No Hit CAAACTAGAGGTTACCAAAGAACCATGCATAGCACTGAATAGGGAGCCGCCG 5 0.125 No Hit GTGAAGATACGTTGTTAGGTGCTCCATTTTATTTTCCCATTGAGGCCGAACC 5 0.125 No Hit GCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGATCA 5 0.125 No Hit CCGCAGAAGTAGGAATAATGGCACCAGAAATGATATTGTTTCCATAAAGTAG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.025 0.0 0.0 0.0 0.0 34 0.025 0.0 0.0 0.0 0.0 35 0.025 0.0 0.0 0.0 0.0 36 0.025 0.0 0.0 0.0 0.0 37 0.025 0.0 0.0 0.0 0.0 38 0.025 0.0 0.0 0.0 0.0 39 0.025 0.0 0.0 0.0 0.0 40 0.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra Read 200000 spots for SRR5423432.sra Written 200000 spots for SRR5423432.sra SRR ids: ['SRR5423432.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_lnx9awkv SRR5423432.sra spots: 4000000 blocks: [[1, 200000], [200001, 400000], [400001, 600000], [600001, 800000], [800001, 1000000], [1000001, 1200000], [1200001, 1400000], [1400001, 1600000], [1600001, 1800000], [1800001, 2000000], [2000001, 2200000], [2200001, 2400000], [2400001, 2600000], [2600001, 2800000], [2800001, 3000000], [3000001, 3200000], [3200001, 3400000], [3400001, 3600000], [3600001, 3800000], [3800001, 4000000]] SRR5423432 file size 704019 SRR5423432 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423432 SRR5423432_1.fastq Input file: SRR5423432_1.fastq trimmed: SRR5423432-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Wed Feb 12 11:21:04 2025 >> started Wed Feb 12 11:21:06 2025 >> done (2.016s) 4000000 reads processed; of these: 121 ( 0.00%) short reads filtered out after trimming by size control 65 ( 0.00%) empty reads filtered out after trimming by size control 3999814 (100.00%) reads available; of these: 138759 ( 3.47%) trimmed reads available after processing 3861055 (96.53%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 10 0.00% 19 5 0.00% 20 7 0.00% 21 2 0.00% 22 1 0.00% 23 1 0.00% 24 0 0.00% 25 2 0.00% 26 0 0.00% 27 5 0.00% 28 5 0.00% 29 3 0.00% 30 9 0.00% 31 11 0.00% 32 2 0.00% 33 20 0.00% 34 21 0.00% 35 24 0.00% 36 23 0.00% 37 30 0.00% 38 35 0.00% 39 53 0.00% 40 61 0.00% 41 105 0.00% 42 151 0.00% 43 193 0.00% 44 299 0.01% 45 454 0.01% 46 593 0.01% 47 1042 0.03% 48 1881 0.05% 49 4322 0.11% 50 14071 0.35% 51 115318 2.88% 52 3861055 96.53% 3999814 reads passed initial QC criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=28 prefix-density=0.47 prefix-fanout=2.0 sequence=TGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGAC criterion=fanout-score sequence-density=0.02 sequence-density-rank=28 fanout-score=23.34 fanout-score-rank=1 prefix-density=0.31 prefix-fanout=1.2 sequence=CATTTTATTTCTATCTTTCCATACATAACCAAAAAATGGAAAAGATTCTTCAAGAGTCTCGGGTCCTAGAAGTGCATGATAAATACCGCCAAAGCCCAATACTGCAGAGGAAATTAAGTGAAGTACGCCAGATACAAAGTATGGAAAGGTGTCTATGACTTCCCCGCCAGGACCCACCCCCCAACCTAGAGTAGCTAGGTGGGGAAGTAAAATTAATCCTTGTTCATACATTGGCTTCTCCGGTACGAAATGGGCCACTTCAAATAGGTTCATTGCTCCGGCCCAGAATACGATTAATCCAGCATGGGCTACATGAGCTCCCAGCAG Started job on | Feb 12 11:21:16 Started mapping on | Feb 12 11:21:17 Finished on | Feb 12 11:21:28 Mapping speed, Million of reads per hour | 1309.03 Number of input reads | 3999814 Average input read length | 51 UNIQUE READS: Uniquely mapped reads number | 3119358 Uniquely mapped reads % | 77.99% Average mapped length | 51.76 Number of splices: Total | 284010 Number of splices: Annotated (sjdb) | 279396 Number of splices: GT/AG | 277475 Number of splices: GC/AG | 5253 Number of splices: AT/AC | 787 Number of splices: Non-canonical | 495 Mismatch rate per base, % | 0.65% Deletion rate per base | 0.01% Deletion average length | 2.21 Insertion rate per base | 0.00% Insertion average length | 1.32 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 718630 % of reads mapped to multiple loci | 17.97% Number of reads mapped to too many loci | 81280 % of reads mapped to too many loci | 2.03% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.00% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 161826 161826 161826 N_multimapping 718630 718630 718630 N_noFeature 394593 3074873 427834 N_ambiguous 22017 174 10614 UnstrandedReadsAssigned:2702748 PositiveStrandReadsAssigned:44311 NegativeStrandReadsAssigned:2680910 Dataset is classified negative stranded MeadianReadLen=52 20thPercentileLength=52 echo kmer=47 SRR5423432 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR5423432-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,999,814 reads, 3,162,131 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,149 rounds 52401 SRR5423432.ke.tsv 34699 SRR5423432.se.tsv 87100 total ==> SRR5423432.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 122 18.5097 Potri.005G024800.1.v4.1 1035 936 14.0416 4.36773 Potri.004G059700.1.v4.1 961 862 2 0.675519 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 51.5724 5.27961 Potri.016G087400.1.v4.1 270 171 19 32.3498 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 1 0.173924 Potri.012G127500.1.v4.1 977 878 7 2.32123 ==> SRR5423432.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 2 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 28 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 2 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR5423432 completed mapping pipeline successfully