Starting /dee2/code/volunteer_pipeline.sh SRR5423433 current disk space = 3051267248128 free memory = 1582375108 SRR5423433 SRAfilesize fc827a43e94968b75fdb9a322890b555 SRR5423433.sra SRR5423433.sra file validated SRR5423433 is single end SRR5423433 is conventional basespace SRR5423433 read1 length is 52 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5423433_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.023 34.0 33.0 34.0 31.0 34.0 2 33.167 34.0 34.0 34.0 31.0 34.0 3 33.2105 34.0 34.0 34.0 31.0 34.0 4 36.47 37.0 37.0 37.0 35.0 37.0 5 36.47025 37.0 37.0 37.0 35.0 37.0 6 36.508 37.0 37.0 37.0 35.0 37.0 7 36.45525 37.0 37.0 37.0 35.0 37.0 8 36.48775 37.0 37.0 37.0 35.0 37.0 9 38.35875 39.0 39.0 39.0 37.0 39.0 10 38.33275 39.0 39.0 39.0 37.0 39.0 11 38.3135 39.0 39.0 39.0 37.0 39.0 12 38.33 39.0 39.0 39.0 37.0 39.0 13 38.27875 39.0 39.0 39.0 37.0 39.0 14 39.92625 41.0 40.0 41.0 38.0 41.0 15 39.87 41.0 40.0 41.0 38.0 41.0 16 39.89725 41.0 40.0 41.0 38.0 41.0 17 39.86725 41.0 40.0 41.0 38.0 41.0 18 39.81325 41.0 40.0 41.0 38.0 41.0 19 39.794 41.0 40.0 41.0 38.0 41.0 20 39.8 41.0 40.0 41.0 38.0 41.0 21 39.7275 41.0 40.0 41.0 38.0 41.0 22 39.6915 41.0 40.0 41.0 38.0 41.0 23 39.708 41.0 40.0 41.0 37.0 41.0 24 39.64575 41.0 40.0 41.0 37.0 41.0 25 39.6425 41.0 40.0 41.0 37.0 41.0 26 39.511 41.0 40.0 41.0 37.0 41.0 27 39.532 41.0 40.0 41.0 37.0 41.0 28 39.5075 41.0 40.0 41.0 37.0 41.0 29 39.45075 41.0 40.0 41.0 37.0 41.0 30 39.50925 41.0 40.0 41.0 37.0 41.0 31 39.42075 41.0 40.0 41.0 37.0 41.0 32 39.36375 41.0 40.0 41.0 37.0 41.0 33 39.30475 41.0 39.0 41.0 37.0 41.0 34 39.299 41.0 40.0 41.0 37.0 41.0 35 39.256 41.0 40.0 41.0 36.0 41.0 36 39.11 41.0 39.0 41.0 36.0 41.0 37 39.03725 41.0 39.0 41.0 36.0 41.0 38 39.02325 41.0 39.0 41.0 36.0 41.0 39 38.9895 40.0 39.0 41.0 35.0 41.0 40 38.87025 40.0 39.0 41.0 35.0 41.0 41 38.8575 40.0 39.0 41.0 35.0 41.0 42 38.847 40.0 39.0 41.0 35.0 41.0 43 38.84675 40.0 39.0 41.0 35.0 41.0 44 38.34275 40.0 38.0 41.0 34.0 41.0 45 38.46725 40.0 38.0 41.0 34.0 41.0 46 38.3355 40.0 38.0 41.0 34.0 41.0 47 38.4145 40.0 38.0 41.0 34.0 41.0 48 38.32125 40.0 38.0 41.0 34.0 41.0 49 38.391 40.0 38.0 41.0 34.0 41.0 50 38.2875 40.0 38.0 41.0 34.0 41.0 51 38.144 40.0 38.0 41.0 34.0 41.0 52 36.56825 39.0 36.0 40.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2202 1 0.0 2202 2 0.0 2202 3 0.0 2202 4 0.0 2202 5 0.0 2202 6 0.0 2202 7 0.0 2202 8 0.0 2202 9 0.0 2202 10 0.0 2202 11 0.0 2202 12 0.0 2202 13 0.0 2202 14 0.0 2202 15 0.0 2202 16 0.0 2202 17 0.0 2202 18 0.0 2202 19 0.0 2202 20 0.0 2202 21 0.0 2202 22 0.0 2202 23 0.0 2202 24 0.0 2202 25 0.0 2202 26 0.0 2202 27 0.0 2202 28 0.0 2202 29 0.0 2202 30 0.0 2202 31 0.0 2202 32 0.0 2202 33 0.0 2202 34 0.0 2202 35 0.0 2202 36 0.0 2202 37 0.0 2202 38 0.0 2202 39 0.0 2202 40 0.0 2202 41 0.0 2202 42 0.0 2202 43 0.0 2202 44 0.0 2202 45 0.0 2202 46 0.0 2202 47 0.0 2202 48 0.0 2202 49 0.0 2202 50 0.0 2202 51 0.0 2202 52 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 0.0 10 1.0 11 1.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 2.0 22 1.0 23 4.0 24 6.0 25 5.0 26 7.0 27 17.0 28 13.0 29 17.0 30 22.0 31 29.0 32 41.0 33 53.0 34 65.0 35 96.0 36 191.0 37 243.0 38 645.0 39 2526.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.078156312625254 9.769539078156313 5.736472945891784 45.41583166332666 2 24.9 13.4 34.475 27.224999999999998 3 21.55 16.85 23.75 37.85 4 27.1 24.825 21.025 27.05 5 24.0 29.799999999999997 24.125 22.075 6 19.15 31.924999999999997 24.875 24.05 7 16.275000000000002 22.8 40.65 20.275000000000002 8 18.9 22.3 29.7 29.099999999999998 9 19.35 20.474999999999998 32.75 27.425 10 19.1 39.300000000000004 22.725 18.875 11 23.225 28.1 20.875 27.800000000000004 12 22.25 24.55 25.55 27.650000000000002 13 19.825 27.900000000000002 26.575 25.7 14 21.325 28.475 26.224999999999998 23.974999999999998 15 21.7 26.575 26.450000000000003 25.275 16 22.45 26.474999999999998 25.825 25.25 17 23.175 26.674999999999997 25.75 24.4 18 21.525 26.75 26.375 25.35 19 21.675 26.25 26.85 25.224999999999998 20 21.675 26.200000000000003 26.575 25.55 21 21.425 26.05 26.625 25.900000000000002 22 22.175 26.974999999999998 25.45 25.4 23 21.6 27.375 25.324999999999996 25.7 24 20.625 27.150000000000002 24.925 27.3 25 21.75 27.525 25.7 25.025 26 22.650000000000002 26.924999999999997 26.05 24.375 27 20.925 27.125 26.3 25.650000000000002 28 21.575 27.55 25.900000000000002 24.975 29 20.75 27.125 27.400000000000002 24.725 30 21.099999999999998 25.525 26.150000000000002 27.224999999999998 31 21.575 27.35 25.1 25.974999999999998 32 20.974999999999998 27.500000000000004 25.624999999999996 25.900000000000002 33 19.900000000000002 26.525 27.150000000000002 26.424999999999997 34 21.4 27.575 25.15 25.874999999999996 35 20.95 27.05 25.95 26.05 36 21.025 27.025 24.075 27.875 37 21.85 26.775 26.275 25.1 38 21.7 26.525 25.5 26.275 39 22.15 26.424999999999997 25.724999999999998 25.7 40 22.7 25.95 26.55 24.8 41 22.35 24.725 26.25 26.674999999999997 42 19.900000000000002 25.275 26.650000000000002 28.175 43 21.080270067516878 27.181795448862218 25.656414103525883 26.081520380095025 44 22.900000000000002 27.1 24.9 25.1 45 22.18054513628407 25.881470367591895 25.906476619154787 26.03150787696924 46 22.705676419104776 26.93173293323331 25.731432858214554 24.63115778944736 47 23.111555777888945 26.588294147073537 25.11255627813907 25.18759379689845 48 21.48037009252313 26.63165791447862 24.63115778944736 27.25681420355089 49 22.280570142535634 25.581395348837212 25.30632658164541 26.831707926981746 50 22.1055263815954 26.30657664416104 26.106526631657918 25.481370342585645 51 21.980495123780948 25.95648912228057 24.956239059764943 27.106776694173547 52 23.25 26.125 25.35 25.275 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.0 10 1.0 11 1.0 12 1.0 13 0.5 14 0.0 15 0.0 16 0.5 17 1.0 18 2.5 19 4.0 20 4.0 21 4.0 22 4.5 23 5.0 24 7.5 25 10.0 26 15.0 27 20.0 28 24.0 29 28.0 30 41.5 31 55.0 32 62.0 33 69.0 34 87.0 35 105.0 36 136.0 37 167.0 38 185.5 39 216.0 40 228.0 41 228.5 42 229.0 43 265.5 44 302.0 45 308.0 46 314.0 47 318.0 48 322.0 49 333.5 50 345.0 51 345.5 52 346.0 53 343.5 54 341.0 55 301.5 56 262.0 57 217.5 58 173.0 59 169.0 60 165.0 61 139.5 62 114.0 63 90.0 64 59.0 65 52.0 66 41.5 67 31.0 68 23.0 69 15.0 70 11.5 71 8.0 72 6.0 73 4.0 74 4.0 75 4.0 76 3.0 77 2.0 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 1.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.025 44 0.0 45 0.025 46 0.025 47 0.05 48 0.025 49 0.025 50 0.025 51 0.025 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.825 #Duplication Level Percentage of deduplicated Percentage of total 1 93.80804953560371 83.325 2 3.349282296650718 5.949999999999999 3 1.350971010413735 3.5999999999999996 4 0.7880664227413453 2.8000000000000003 5 0.3658879819870532 1.625 6 0.11258091753447791 0.6 7 0.056290458767238954 0.35000000000000003 8 0.056290458767238954 0.4 9 0.028145229383619477 0.22499999999999998 >10 0.08443568815085843 1.125 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGC 19 0.475 No Hit CCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACT 16 0.4 No Hit GGGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCG 10 0.25 No Hit CTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTGGCCCGCAGCAGTA 9 0.22499999999999998 No Hit GTCCCTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAAC 8 0.2 No Hit GGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGA 8 0.2 No Hit CCCACTTTTTGGTCTTAAGAATGCTGGTTTTAAGAATGAGTGATTGCCCTTC 7 0.17500000000000002 No Hit CTCAGCCTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGAGGC 7 0.17500000000000002 No Hit GCCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTAC 6 0.15 No Hit GTCGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAAT 6 0.15 No Hit CTCGGTTGCTGGAACCTCCATGACTCCAGTGTAGACATGGCTCTTCTCAGTC 6 0.15 No Hit GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGTA 6 0.15 No Hit GGGCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGAT 5 0.125 No Hit CCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCCCTGCTTCCTCGGGC 5 0.125 No Hit GCCGCCGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGACGCATACCCA 5 0.125 No Hit GTCCGCGACCCCTGGATGCCGAAGGCGTCCTTGGGGCGATCTCGTAGTTCCT 5 0.125 No Hit CTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGCCC 5 0.125 No Hit CCCGAAGCCACGGGTAGAACACCCGGTAGAGAGACCCAATCTTGAGTGAAAT 5 0.125 No Hit CCCGAACACAGCTTACAACTTTCATCGTACTGTGCTCTCCAAAGAGCAACTC 5 0.125 No Hit CTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAACGGCA 5 0.125 No Hit CATGAATGTGATGGACCAAAAAATCCGCGGTTCCTAATGGAATAGGTAACAA 5 0.125 No Hit GTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCC 5 0.125 No Hit GCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGATCA 5 0.125 No Hit GCCGAACCTAAACCTGTGCTCGAGAGATAGCTGTCCATACACTGATAAGGGA 5 0.125 No Hit GCCAGATACAAAGTATGGAAAGGTGTCTATGACTTCCCCGCCAGGACCCACC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10 0.025 0.0 0.0 0.0 0.0 11 0.025 0.0 0.0 0.0 0.0 12 0.025 0.0 0.0 0.0 0.0 13 0.025 0.0 0.0 0.0 0.0 14 0.025 0.0 0.0 0.0 0.0 15 0.025 0.0 0.0 0.0 0.0 16 0.025 0.0 0.0 0.0 0.0 17 0.025 0.0 0.0 0.0 0.0 18 0.025 0.0 0.0 0.0 0.0 19 0.025 0.0 0.0 0.0 0.0 20 0.025 0.0 0.0 0.0 0.0 21 0.025 0.0 0.0 0.0 0.0 22 0.025 0.0 0.0 0.0 0.0 23 0.025 0.0 0.0 0.0 0.0 24 0.025 0.0 0.0 0.0 0.0 25 0.025 0.0 0.0 0.0 0.0 26 0.025 0.0 0.0 0.0 0.0 27 0.025 0.0 0.0 0.0 0.0 28 0.025 0.0 0.0 0.0 0.0 29 0.025 0.0 0.0 0.0 0.0 30 0.025 0.0 0.0 0.0 0.0 31 0.025 0.0 0.0 0.0 0.0 32 0.025 0.0 0.0 0.0 0.0 33 0.025 0.0 0.0 0.0 0.0 34 0.025 0.0 0.0 0.0 0.0 35 0.025 0.0 0.0 0.0 0.0 36 0.05 0.0 0.0 0.0 0.0 37 0.05 0.0 0.0 0.0 0.0 38 0.05 0.0 0.0 0.0 0.0 39 0.05 0.0 0.0 0.0 0.0 40 0.05 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra Read 200000 spots for SRR5423433.sra Written 200000 spots for SRR5423433.sra SRR ids: ['SRR5423433.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_lb6yytcz SRR5423433.sra spots: 4000000 blocks: [[1, 200000], [200001, 400000], [400001, 600000], [600001, 800000], [800001, 1000000], [1000001, 1200000], [1200001, 1400000], [1400001, 1600000], [1600001, 1800000], [1800001, 2000000], [2000001, 2200000], [2200001, 2400000], [2400001, 2600000], [2600001, 2800000], [2800001, 3000000], [3000001, 3200000], [3200001, 3400000], [3400001, 3600000], [3600001, 3800000], [3800001, 4000000]] SRR5423433 file size 703950 SRR5423433 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423433 SRR5423433_1.fastq Input file: SRR5423433_1.fastq trimmed: SRR5423433-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Wed Feb 12 12:01:36 2025 >> started Wed Feb 12 12:01:38 2025 >> done (1.930s) 4000000 reads processed; of these: 172 ( 0.00%) short reads filtered out after trimming by size control 80 ( 0.00%) empty reads filtered out after trimming by size control 3999748 (99.99%) reads available; of these: 94533 ( 2.36%) trimmed reads available after processing 3905215 (97.64%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 12 0.00% 19 5 0.00% 20 7 0.00% 21 0 0.00% 22 1 0.00% 23 0 0.00% 24 4 0.00% 25 3 0.00% 26 5 0.00% 27 3 0.00% 28 6 0.00% 29 6 0.00% 30 3 0.00% 31 11 0.00% 32 8 0.00% 33 13 0.00% 34 18 0.00% 35 29 0.00% 36 30 0.00% 37 35 0.00% 38 46 0.00% 39 63 0.00% 40 83 0.00% 41 125 0.00% 42 164 0.00% 43 214 0.01% 44 390 0.01% 45 492 0.01% 46 643 0.02% 47 992 0.02% 48 1884 0.05% 49 4153 0.10% 50 11733 0.29% 51 73352 1.83% 52 3905215 97.64% 3999748 reads passed initial QC criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=29 prefix-density=0.46 prefix-fanout=2.0 sequence=TGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGAC criterion=fanout-score sequence-density=0.02 sequence-density-rank=31 fanout-score=23.97 fanout-score-rank=1 prefix-density=0.31 prefix-fanout=1.2 sequence=CATTTTATTTCTATCTTTCCATACATAACCAAAAAATGGAAAAGATTCTTCAAGAGTCTCGGGTCCTAGAAGTGCATGATAAATACCGCCAAAGCCCAATACTGCAGAGGAAATTAAGTGAAGTACGCCAGATACAAAGTATGGAAAGGTGTCTATGACTTCCCCGCCAGGACCCACCCCCCAACCTAGAGTAGCTAGGTGGGGAAGTAAAATTAATCCTTGTTCATACATTGGCTTCTCCGGTACGAAATGGGCCACTTCAAATAGGTTCATTGCTCCGGCCCAGAATACGATTAATCCAGCATGGGCTACATGAGCTCCCAGCAG Started job on | Feb 12 12:01:51 Started mapping on | Feb 12 12:01:51 Finished on | Feb 12 12:01:56 Mapping speed, Million of reads per hour | 2879.82 Number of input reads | 3999748 Average input read length | 51 UNIQUE READS: Uniquely mapped reads number | 3134367 Uniquely mapped reads % | 78.36% Average mapped length | 51.78 Number of splices: Total | 287311 Number of splices: Annotated (sjdb) | 282680 Number of splices: GT/AG | 280718 Number of splices: GC/AG | 5304 Number of splices: AT/AC | 797 Number of splices: Non-canonical | 492 Mismatch rate per base, % | 0.33% Deletion rate per base | 0.01% Deletion average length | 2.19 Insertion rate per base | 0.00% Insertion average length | 1.35 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 712456 % of reads mapped to multiple loci | 17.81% Number of reads mapped to too many loci | 82778 % of reads mapped to too many loci | 2.07% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.74% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 152925 152925 152925 N_multimapping 712456 712456 712456 N_noFeature 395212 3088921 429258 N_ambiguous 21966 181 10404 UnstrandedReadsAssigned:2717189 PositiveStrandReadsAssigned:45265 NegativeStrandReadsAssigned:2694705 Dataset is classified negative stranded MeadianReadLen=52 20thPercentileLength=52 echo kmer=47 SRR5423433 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR5423433-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,999,748 reads, 3,275,887 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,145 rounds 52401 SRR5423433.ke.tsv 34699 SRR5423433.se.tsv 87100 total ==> SRR5423433.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 146 21.3859 Potri.005G024800.1.v4.1 1035 936 18.0454 5.41925 Potri.004G059700.1.v4.1 961 862 7 2.28265 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 39.2189 3.87628 Potri.016G087400.1.v4.1 270 171 12 19.7258 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 1 0.167917 Potri.012G127500.1.v4.1 977 878 6 1.92091 ==> SRR5423433.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 0 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 36 Potri.001G212900.v4.1 3 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 2 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR5423433 completed mapping pipeline successfully