Starting /dee2/code/volunteer_pipeline.sh SRR5423437 current disk space = 3051253145600 free memory = 1581976228 SRR5423437 SRAfilesize f1ddf9f68fa9939009c39bc13fad2cb7 SRR5423437.sra SRR5423437.sra file validated SRR5423437 is single end SRR5423437 is conventional basespace SRR5423437 read1 length is 52 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5423437_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.613 31.0 31.0 34.0 30.0 34.0 2 31.851 31.0 31.0 34.0 30.0 34.0 3 31.88425 31.0 31.0 34.0 30.0 34.0 4 34.88875 37.0 35.0 37.0 32.0 37.0 5 35.35325 37.0 35.0 37.0 33.0 37.0 6 35.08125 37.0 35.0 37.0 32.0 37.0 7 35.08875 37.0 35.0 37.0 32.0 37.0 8 34.94625 37.0 35.0 37.0 32.0 37.0 9 36.92675 39.0 37.0 39.0 33.0 39.0 10 36.897 39.0 37.0 39.0 33.0 39.0 11 36.7945 39.0 37.0 39.0 33.0 39.0 12 36.9025 39.0 37.0 39.0 33.0 39.0 13 36.73675 39.0 37.0 39.0 32.0 39.0 14 37.6695 40.0 37.0 41.0 32.0 41.0 15 37.8005 40.0 37.0 41.0 32.0 41.0 16 37.531 39.0 36.0 41.0 32.0 41.0 17 37.57925 39.0 37.0 41.0 32.0 41.0 18 37.35075 39.0 36.0 41.0 32.0 41.0 19 37.80125 39.0 37.0 41.0 33.0 41.0 20 37.564 39.0 36.0 41.0 32.0 41.0 21 37.80775 40.0 37.0 41.0 32.0 41.0 22 37.91075 40.0 37.0 41.0 33.0 41.0 23 37.80625 40.0 37.0 41.0 32.0 41.0 24 37.848 40.0 37.0 41.0 33.0 41.0 25 37.674 40.0 37.0 41.0 32.0 41.0 26 37.7285 40.0 37.0 41.0 32.0 41.0 27 37.65775 40.0 37.0 41.0 32.0 41.0 28 37.71325 40.0 37.0 41.0 32.0 41.0 29 37.773 40.0 37.0 41.0 33.0 41.0 30 37.32675 40.0 36.0 41.0 31.0 41.0 31 37.416 39.0 36.0 41.0 31.0 41.0 32 37.33975 39.0 36.0 41.0 31.0 41.0 33 37.49975 39.0 37.0 41.0 31.0 41.0 34 37.43775 39.0 37.0 41.0 32.0 41.0 35 37.3155 39.0 36.0 41.0 31.0 41.0 36 37.318 39.0 36.0 41.0 31.0 41.0 37 37.1165 39.0 36.0 41.0 30.0 41.0 38 36.9935 39.0 36.0 41.0 30.0 41.0 39 37.14 39.0 36.0 41.0 31.0 41.0 40 37.2 39.0 36.0 41.0 31.0 41.0 41 37.12175 39.0 36.0 41.0 31.0 41.0 42 37.07375 39.0 36.0 40.0 31.0 41.0 43 36.906 39.0 36.0 40.0 30.0 41.0 44 36.79875 39.0 35.0 40.0 30.0 41.0 45 36.717 39.0 35.0 40.0 30.0 41.0 46 36.62925 39.0 35.0 40.0 30.0 41.0 47 36.603 39.0 35.0 40.0 30.0 41.0 48 36.52425 39.0 35.0 40.0 30.0 41.0 49 36.47 39.0 35.0 40.0 30.0 41.0 50 36.34275 39.0 35.0 40.0 29.0 41.0 51 36.33025 39.0 35.0 40.0 29.0 41.0 52 35.453 38.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1113 1 0.0 1113 2 0.0 1113 3 0.0 1113 4 0.0 1113 5 0.0 1113 6 0.0 1113 7 0.0 1113 8 0.0 1113 9 0.0 1113 10 0.0 1113 11 0.0 1113 12 0.0 1113 13 0.0 1113 14 0.0 1113 15 0.0 1113 16 0.0 1113 17 0.0 1113 18 0.0 1113 19 0.0 1113 20 0.0 1113 21 0.0 1113 22 0.0 1113 23 0.0 1113 24 0.0 1113 25 0.0 1113 26 0.0 1113 27 0.0 1113 28 0.0 1113 29 0.0 1113 30 0.0 1113 31 0.0 1113 32 0.0 1113 33 0.0 1113 34 0.0 1113 35 0.0 1113 36 0.0 1113 37 0.0 1113 38 0.0 1113 39 0.0 1113 40 0.0 1113 41 0.0 1113 42 0.0 1113 43 0.0 1113 44 0.0 1113 45 0.0 1113 46 0.0 1113 47 0.0 1113 48 0.0 1113 49 0.0 1113 50 0.0 1113 51 0.0 1113 52 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 2.0 23 8.0 24 8.0 25 9.0 26 23.0 27 26.0 28 55.0 29 80.0 30 88.0 31 113.0 32 112.0 33 190.0 34 244.0 35 307.0 36 370.0 37 472.0 38 760.0 39 1133.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.97896845267902 10.240360540811217 5.383074611917877 48.397596394591886 2 22.425 14.124999999999998 36.175000000000004 27.275 3 21.65 17.575 23.375 37.4 4 25.5 25.324999999999996 19.525000000000002 29.65 5 23.150000000000002 31.974999999999998 23.9 20.974999999999998 6 20.849999999999998 32.275 24.15 22.725 7 15.8 21.85 41.9 20.45 8 17.95 23.45 29.45 29.15 9 17.2 22.05 32.875 27.875 10 18.175 37.574999999999996 23.525 20.724999999999998 11 23.05 27.625 21.95 27.375 12 21.725 25.25 26.35 26.674999999999997 13 19.125 27.650000000000002 27.0 26.224999999999998 14 20.225 28.199999999999996 28.275 23.3 15 21.025 27.525 26.450000000000003 25.0 16 20.825 26.400000000000002 27.275 25.5 17 21.775 26.674999999999997 27.025 24.525 18 20.925 27.200000000000003 26.0 25.874999999999996 19 21.4 27.474999999999998 24.525 26.6 20 20.875 25.95 25.900000000000002 27.275 21 20.150000000000002 26.0 26.650000000000002 27.200000000000003 22 20.525 27.700000000000003 24.349999999999998 27.425 23 20.5 28.175 24.85 26.474999999999998 24 22.125 26.224999999999998 26.674999999999997 24.975 25 21.375 26.75 26.174999999999997 25.7 26 22.85 27.825 25.674999999999997 23.65 27 21.675 27.3 25.05 25.974999999999998 28 22.400000000000002 26.5 25.474999999999998 25.624999999999996 29 20.05 27.875 28.000000000000004 24.075 30 20.9 25.575 27.400000000000002 26.125 31 21.325 27.625 25.85 25.2 32 22.45 26.974999999999998 25.874999999999996 24.7 33 21.775 25.8 27.450000000000003 24.975 34 20.775 26.424999999999997 26.0 26.8 35 21.175 26.5 24.7 27.625 36 21.4 26.375 24.625 27.6 37 20.875 25.75 26.55 26.825 38 21.15 27.0 26.724999999999998 25.124999999999996 39 20.575 25.55 25.6 28.275 40 22.775000000000002 26.650000000000002 24.2 26.375 41 22.6 26.974999999999998 24.7 25.724999999999998 42 21.175 25.7 25.650000000000002 27.474999999999998 43 21.075 27.900000000000002 25.25 25.775 44 22.925 26.075 25.8 25.2 45 24.175 24.3 26.125 25.4 46 22.95 26.1 24.975 25.974999999999998 47 22.6 26.200000000000003 26.05 25.15 48 21.6 27.175 25.025 26.200000000000003 49 21.224999999999998 26.875 25.15 26.75 50 23.974999999999998 26.375 24.7 24.95 51 21.7 25.1 25.5 27.700000000000003 52 21.925 26.325 25.25 26.5 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 1.0 16 2.5 17 4.0 18 5.0 19 6.0 20 6.5 21 7.0 22 10.5 23 14.0 24 13.0 25 12.0 26 13.0 27 14.0 28 22.5 29 31.0 30 39.0 31 47.0 32 52.5 33 58.0 34 86.5 35 115.0 36 123.0 37 131.0 38 179.5 39 219.0 40 210.0 41 236.5 42 263.0 43 290.0 44 317.0 45 308.0 46 299.0 47 339.0 48 379.0 49 344.0 50 309.0 51 335.0 52 361.0 53 336.5 54 312.0 55 275.5 56 239.0 57 212.5 58 186.0 59 171.0 60 156.0 61 139.0 62 122.0 63 95.5 64 55.5 65 42.0 66 33.5 67 25.0 68 20.0 69 15.0 70 13.0 71 11.0 72 7.0 73 3.0 74 3.0 75 3.0 76 3.0 77 3.0 78 2.5 79 2.0 80 1.5 81 1.0 82 1.0 83 1.0 84 0.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.325 #Duplication Level Percentage of deduplicated Percentage of total 1 94.16926125106141 83.175 2 3.141805830738749 5.55 3 1.1038777243136144 2.9250000000000003 4 0.5943956977073309 2.1 5 0.22643645626945935 1.0 6 0.31135012737050666 1.6500000000000001 7 0.22643645626945935 1.4000000000000001 8 0.11321822813472968 0.8 9 0.0 0.0 >10 0.11321822813472968 1.4000000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGA 17 0.42500000000000004 No Hit CGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGC 16 0.4 No Hit CCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACT 12 0.3 No Hit CTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGCCC 11 0.27499999999999997 No Hit GGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGT 8 0.2 No Hit GTCGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAAT 8 0.2 No Hit GTCCCTTATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCA 8 0.2 No Hit CTTATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCATGTA 8 0.2 No Hit CTCGTATTTTCCCCTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTG 7 0.17500000000000002 No Hit CCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCCCT 7 0.17500000000000002 No Hit GTCAGAGCAGGCATATGCCAAACGTGAATACCCCCCGAAGCCACGGGTAGAA 7 0.17500000000000002 No Hit CTCGGGTCCTAGAAGTGCATGATAAATACCGCCAAAGCCCAATACTGCAGAG 7 0.17500000000000002 No Hit CGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTG 7 0.17500000000000002 No Hit CCTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAACGGC 7 0.17500000000000002 No Hit GTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGC 7 0.17500000000000002 No Hit GCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGATCA 7 0.17500000000000002 No Hit CCAGCCTTGAATCCAACACCTGCTTTAGTCTCTGTTTGTGGTGACATAAGCC 6 0.15 No Hit GTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCG 6 0.15 No Hit CACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCCCTG 6 0.15 No Hit GTCCTAGAAGTGCATGATAAATACCGCCAAAGCCCAATACTGCAGAGGAAAT 6 0.15 No Hit CTCGGTTGCTGGAACCTCCATGACTCCAGTGTAGACATGGCTCTTCTCAGTC 6 0.15 No Hit GTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCC 6 0.15 No Hit GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTAAAAGTA 6 0.15 No Hit CTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTGGCCCGCAGCAGTA 6 0.15 No Hit CCGTCGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGA 6 0.15 No Hit AGCACGGTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCA 6 0.15 No Hit CTCAGCCTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGAGGC 6 0.15 No Hit CTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTTTCCCTGATCAAACTAG 5 0.125 No Hit CCCTACGCCACACACATGACGGTTTACGTGCTTAATGACCGCATGTGCAGGT 5 0.125 No Hit GCTCAGCCTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGAGG 5 0.125 No Hit CCCTGATCAAACTAGAGGTTACCAAAGAACCATGCATAGCACTGAATAGGGA 5 0.125 No Hit CTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAACGGCA 5 0.125 No Hit GGCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGATC 5 0.125 No Hit CTCCAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACT 5 0.125 No Hit CTCGATTAGCAACGGCACCGGGTGCATTTCCCCAAGGGTGCCCTAAAGTTCC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra Read 200000 spots for SRR5423437.sra Written 200000 spots for SRR5423437.sra SRR ids: ['SRR5423437.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ev7nvd0v SRR5423437.sra spots: 4000000 blocks: [[1, 200000], [200001, 400000], [400001, 600000], [600001, 800000], [800001, 1000000], [1000001, 1200000], [1200001, 1400000], [1400001, 1600000], [1600001, 1800000], [1800001, 2000000], [2000001, 2200000], [2200001, 2400000], [2400001, 2600000], [2600001, 2800000], [2800001, 3000000], [3000001, 3200000], [3200001, 3400000], [3400001, 3600000], [3600001, 3800000], [3800001, 4000000]] SRR5423437 file size 704034 SRR5423437 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423437 SRR5423437_1.fastq Input file: SRR5423437_1.fastq trimmed: SRR5423437-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Wed Feb 12 12:18:53 2025 >> started Wed Feb 12 12:18:56 2025 >> done (2.783s) 4000000 reads processed; of these: 190 ( 0.00%) short reads filtered out after trimming by size control 232 ( 0.01%) empty reads filtered out after trimming by size control 3999578 (99.99%) reads available; of these: 94525 ( 2.36%) trimmed reads available after processing 3905053 (97.64%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 10 0.00% 19 5 0.00% 20 6 0.00% 21 0 0.00% 22 0 0.00% 23 0 0.00% 24 2 0.00% 25 3 0.00% 26 2 0.00% 27 3 0.00% 28 3 0.00% 29 5 0.00% 30 4 0.00% 31 6 0.00% 32 12 0.00% 33 14 0.00% 34 11 0.00% 35 17 0.00% 36 33 0.00% 37 34 0.00% 38 41 0.00% 39 55 0.00% 40 82 0.00% 41 112 0.00% 42 145 0.00% 43 171 0.00% 44 379 0.01% 45 388 0.01% 46 593 0.01% 47 1096 0.03% 48 2066 0.05% 49 4220 0.11% 50 12156 0.30% 51 72851 1.82% 52 3905053 97.64% 3999578 reads passed initial QC criterion=sequence-density sequence-density=0.18 sequence-density-rank=1 fanout-score=6.62 fanout-score-rank=7 prefix-density=0.78 prefix-fanout=1.5 sequence=ACGTGCTTAATACGTGCTTAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=29 fanout-score=53.04 fanout-score-rank=1 prefix-density=0.40 prefix-fanout=1.1 sequence=CCCCTCTGCCACAAATTTTGACGTAGGCTCTCCCTCTTGGGGAGACCTTAG Started job on | Feb 12 12:19:09 Started mapping on | Feb 12 12:19:09 Finished on | Feb 12 12:19:15 Mapping speed, Million of reads per hour | 2399.75 Number of input reads | 3999578 Average input read length | 51 UNIQUE READS: Uniquely mapped reads number | 3142020 Uniquely mapped reads % | 78.56% Average mapped length | 51.73 Number of splices: Total | 300087 Number of splices: Annotated (sjdb) | 295275 Number of splices: GT/AG | 293539 Number of splices: GC/AG | 5261 Number of splices: AT/AC | 817 Number of splices: Non-canonical | 470 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.01% Deletion average length | 2.36 Insertion rate per base | 0.00% Insertion average length | 1.38 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 676180 % of reads mapped to multiple loci | 16.91% Number of reads mapped to too many loci | 106431 % of reads mapped to too many loci | 2.66% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.86% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 181378 181378 181378 N_multimapping 676180 676180 676180 N_noFeature 406794 3095197 443220 N_ambiguous 19937 144 9408 UnstrandedReadsAssigned:2715289 PositiveStrandReadsAssigned:46679 NegativeStrandReadsAssigned:2689392 Dataset is classified negative stranded MeadianReadLen=52 20thPercentileLength=52 echo kmer=47 SRR5423437 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR5423437-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,999,578 reads, 3,238,781 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,134 rounds 52401 SRR5423437.ke.tsv 34699 SRR5423437.se.tsv 87100 total ==> SRR5423437.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 138 19.6551 Potri.005G024800.1.v4.1 1035 936 20 5.84018 Potri.004G059700.1.v4.1 961 862 2 0.634154 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 67.409 6.47829 Potri.016G087400.1.v4.1 270 171 12 19.1804 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 0 0 Potri.012G127500.1.v4.1 977 878 3 0.933896 ==> SRR5423437.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 1 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 37 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR5423437 completed mapping pipeline successfully