Starting /dee2/code/volunteer_pipeline.sh SRR5423441
    current disk space = 3051203067904
    free memory = 1573786836 
SRR5423441 SRAfilesize
0aee8aa7a120276ba6fc3ba26d688b36  SRR5423441.sra
SRR5423441.sra file validated
SRR5423441 is single end
SRR5423441 is conventional basespace
SRR5423441 read1 length is 52 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5423441_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.23075	30.0	25.0	31.0	25.0	34.0
2	23.261	28.0	16.0	31.0	10.0	34.0
3	25.98525	30.0	16.0	31.0	16.0	34.0
4	30.1085	32.0	28.0	35.0	19.0	37.0
5	33.61175	35.0	33.0	35.0	28.0	37.0
6	34.4395	35.0	35.0	37.0	31.0	37.0
7	34.894	35.0	35.0	37.0	32.0	37.0
8	35.1375	36.0	35.0	37.0	32.0	37.0
9	36.96575	39.0	37.0	39.0	33.0	39.0
10	36.85825	39.0	37.0	39.0	33.0	39.0
11	36.985	39.0	37.0	39.0	33.0	39.0
12	37.0775	39.0	37.0	39.0	33.0	39.0
13	37.05425	39.0	37.0	39.0	33.0	39.0
14	38.06925	40.0	37.0	41.0	33.0	41.0
15	38.03875	40.0	37.0	41.0	33.0	41.0
16	38.01875	40.0	37.0	41.0	33.0	41.0
17	38.13725	40.0	37.0	41.0	33.0	41.0
18	38.0625	40.0	37.0	41.0	33.0	41.0
19	38.0315	40.0	37.0	41.0	33.0	41.0
20	38.194	40.0	37.0	41.0	34.0	41.0
21	37.9465	40.0	37.0	41.0	33.0	41.0
22	38.04375	40.0	37.0	41.0	33.0	41.0
23	37.833	40.0	37.0	41.0	32.0	41.0
24	37.911	40.0	37.0	41.0	33.0	41.0
25	37.93525	40.0	37.0	41.0	33.0	41.0
26	37.88375	40.0	37.0	41.0	33.0	41.0
27	37.85275	40.0	37.0	41.0	32.0	41.0
28	37.48925	39.0	37.0	41.0	32.0	41.0
29	37.81	40.0	37.0	41.0	33.0	41.0
30	37.64975	40.0	37.0	41.0	32.0	41.0
31	37.82475	40.0	37.0	41.0	33.0	41.0
32	37.661	40.0	37.0	41.0	32.0	41.0
33	37.432	40.0	36.0	41.0	31.0	41.0
34	37.5485	40.0	37.0	41.0	32.0	41.0
35	37.42625	40.0	37.0	41.0	31.0	41.0
36	36.81875	39.0	36.0	40.0	30.0	41.0
37	37.02625	39.0	36.0	40.0	30.0	41.0
38	37.083	39.0	36.0	40.0	30.0	41.0
39	36.8985	39.0	35.0	40.0	30.0	41.0
40	37.07675	39.0	36.0	40.0	31.0	41.0
41	36.9515	39.0	35.0	40.0	31.0	41.0
42	36.988	39.0	36.0	40.0	31.0	41.0
43	37.01425	39.0	36.0	40.0	31.0	41.0
44	37.0585	39.0	36.0	40.0	31.0	41.0
45	36.78575	39.0	35.0	40.0	30.0	41.0
46	36.75	39.0	35.0	40.0	30.0	41.0
47	36.799	39.0	35.0	40.0	30.0	41.0
48	36.61175	39.0	35.0	40.0	30.0	41.0
49	36.504	39.0	35.0	40.0	30.0	41.0
50	36.3645	39.0	35.0	40.0	29.0	41.0
51	36.4005	39.0	35.0	40.0	30.0	41.0
52	35.50375	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2113	1	0.0
2113	2	0.0
2113	3	0.0
2113	4	0.0
2113	5	0.0
2113	6	0.0
2113	7	0.0
2113	8	0.0
2113	9	0.0
2113	10	0.0
2113	11	0.0
2113	12	0.0
2113	13	0.0
2113	14	0.0
2113	15	0.0
2113	16	0.0
2113	17	0.0
2113	18	0.0
2113	19	0.0
2113	20	0.0
2113	21	0.0
2113	22	0.0
2113	23	0.0
2113	24	0.0
2113	25	0.0
2113	26	0.0
2113	27	0.0
2113	28	0.0
2113	29	0.0
2113	30	0.0
2113	31	0.0
2113	32	0.0
2113	33	0.0
2113	34	0.0
2113	35	0.0
2113	36	0.0
2113	37	0.0
2113	38	0.0
2113	39	0.0
2113	40	0.0
2113	41	0.0
2113	42	0.0
2113	43	0.0
2113	44	0.0
2113	45	0.0
2113	46	0.0
2113	47	0.0
2113	48	0.0
2113	49	0.0
2113	50	0.0
2113	51	0.0
2113	52	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	0.0
19	1.0
20	1.0
21	2.0
22	4.0
23	8.0
24	10.0
25	18.0
26	22.0
27	28.0
28	58.0
29	57.0
30	91.0
31	136.0
32	151.0
33	177.0
34	270.0
35	329.0
36	447.0
37	651.0
38	855.0
39	682.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.40310465698548	9.664496745117676	6.359539308963445	48.5728592889334
2	39.1	15.024999999999999	27.675	18.2
3	23.575	18.55	22.650000000000002	35.225
4	25.775	25.324999999999996	22.975	25.924999999999997
5	24.8	29.849999999999998	24.175	21.175
6	19.425	33.300000000000004	24.349999999999998	22.925
7	16.6	23.1	41.875	18.425
8	18.6	21.675	30.65	29.075
9	18.625	20.3	34.725	26.35
10	18.375	37.45	24.775	19.400000000000002
11	22.650000000000002	28.499999999999996	20.674999999999997	28.175
12	20.4	24.625	27.750000000000004	27.224999999999998
13	18.15	27.075	29.925	24.85
14	20.05	27.700000000000003	28.449999999999996	23.799999999999997
15	20.8	27.224999999999998	27.450000000000003	24.525
16	20.25	28.050000000000004	26.875	24.825
17	22.05	27.1	26.1	24.75
18	20.95	27.05	25.7	26.3
19	20.65	27.450000000000003	25.75	26.150000000000002
20	21.425	25.95	26.575	26.05
21	20.424999999999997	26.25	27.150000000000002	26.174999999999997
22	21.15	27.425	25.1	26.325
23	21.975	26.974999999999998	24.9	26.150000000000002
24	22.15	28.1	25.025	24.725
25	21.224999999999998	27.725	26.575	24.474999999999998
26	22.650000000000002	27.800000000000004	25.85	23.7
27	20.05	25.874999999999996	27.6	26.474999999999998
28	21.375	27.750000000000004	26.325	24.55
29	21.5	28.325	26.900000000000002	23.275000000000002
30	20.575	26.650000000000002	26.8	25.974999999999998
31	21.725	26.525	26.224999999999998	25.525
32	19.6	28.050000000000004	25.75	26.6
33	20.8	28.075	26.525	24.6
34	20.825	26.174999999999997	28.675	24.325
35	20.625	27.0	26.5	25.874999999999996
36	21.775	26.424999999999997	24.7	27.1
37	20.65	25.924999999999997	26.1	27.325
38	21.65	26.125	25.55	26.674999999999997
39	20.549999999999997	26.924999999999997	26.55	25.974999999999998
40	20.9	27.500000000000004	26.325	25.275
41	21.0	26.375	25.8	26.825
42	21.575	25.674999999999997	27.125	25.624999999999996
43	22.475	26.075	26.55	24.9
44	22.7	26.55	26.200000000000003	24.55
45	22.75	25.825	25.224999999999998	26.200000000000003
46	21.9	27.725	26.275	24.099999999999998
47	22.225	27.375	24.0	26.400000000000002
48	22.375	25.7	25.825	26.1
49	22.25	26.55	24.775	26.424999999999997
50	23.599999999999998	25.0	26.1	25.3
51	21.25	25.6	26.474999999999998	26.674999999999997
52	22.5	26.325	25.25	25.924999999999997
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	2.0
19	4.0
20	5.5
21	7.0
22	8.0
23	9.0
24	11.0
25	13.0
26	15.5
27	18.0
28	29.0
29	40.0
30	43.0
31	46.0
32	73.0
33	100.0
34	105.0
35	110.0
36	144.5
37	179.0
38	187.0
39	226.5
40	258.0
41	269.0
42	280.0
43	290.5
44	301.0
45	302.5
46	304.0
47	323.5
48	343.0
49	337.0
50	331.0
51	328.5
52	326.0
53	318.5
54	311.0
55	272.5
56	234.0
57	204.0
58	174.0
59	157.5
60	141.0
61	123.0
62	105.0
63	86.5
64	50.0
65	32.0
66	27.5
67	23.0
68	19.0
69	15.0
70	13.0
71	11.0
72	11.5
73	12.0
74	8.0
75	4.0
76	3.0
77	2.0
78	1.5
79	1.0
80	1.0
81	1.0
82	1.0
83	1.0
84	1.0
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.45823195458232	88.275
2	2.8926736955934036	5.35
3	0.8921330089213302	2.475
4	0.3514463368477967	1.3
5	0.13517166801838335	0.625
6	0.10813733441470669	0.6
7	0.08110300081103002	0.525
8	0.0	0.0
9	0.027034333603676672	0.22499999999999998
>10	0.054068667207353344	0.625
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGC	13	0.325	No Hit
GGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGA	12	0.3	No Hit
CCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACT	9	0.22499999999999998	No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGCCC	7	0.17500000000000002	No Hit
GGGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCG	7	0.17500000000000002	No Hit
CTCGGTTGCTGGAACCTCCATGACTCCAGTGTAGACATGGCTCTTCTCAGTC	7	0.17500000000000002	No Hit
GGGCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGAT	6	0.15	No Hit
CTCTTGACCAAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTTTCC	6	0.15	No Hit
CTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAACGGCA	6	0.15	No Hit
AGAAGATTCAGCAGCTACTGCGGCCCCTGCTTCCTCGGGCGGAACTCCAGGT	6	0.15	No Hit
CTCGGGTCCTAGAAGTGCATGATAAATACCGCCAAAGCCCAATACTGCAGAG	5	0.125	No Hit
CGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTG	5	0.125	No Hit
GTCCTAGAAGTGCATGATAAATACCGCCAAAGCCCAATACTGCAGAGGAAAT	5	0.125	No Hit
GCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGATCA	5	0.125	No Hit
AGCACGGTTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
Read 200000 spots for SRR5423441.sra
Written 200000 spots for SRR5423441.sra
SRR ids: ['SRR5423441.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_qwztnisg
SRR5423441.sra spots: 4000000
blocks: [[1, 200000], [200001, 400000], [400001, 600000], [600001, 800000], [800001, 1000000], [1000001, 1200000], [1200001, 1400000], [1400001, 1600000], [1600001, 1800000], [1800001, 2000000], [2000001, 2200000], [2200001, 2400000], [2400001, 2600000], [2600001, 2800000], [2800001, 3000000], [3000001, 3200000], [3200001, 3400000], [3400001, 3600000], [3600001, 3800000], [3800001, 4000000]]
SRR5423441 file size 704002
SRR5423441 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423441 SRR5423441_1.fastq
Input file:	SRR5423441_1.fastq
trimmed:	SRR5423441-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Wed Feb 12 13:14:11 2025 >> started

Wed Feb 12 13:14:13 2025 >> done (2.007s)
4000000 reads processed; of these:
    188 ( 0.00%) short reads filtered out after trimming by size control
    208 ( 0.01%) empty reads filtered out after trimming by size control
3999604 (99.99%) reads available; of these:
  95259 ( 2.38%) trimmed reads available after processing
3904345 (97.62%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     12	  0.00%
 19	      5	  0.00%
 20	      9	  0.00%
 21	      0	  0.00%
 22	      0	  0.00%
 23	      0	  0.00%
 24	      3	  0.00%
 25	      2	  0.00%
 26	      3	  0.00%
 27	      5	  0.00%
 28	      2	  0.00%
 29	      5	  0.00%
 30	      8	  0.00%
 31	     11	  0.00%
 32	     12	  0.00%
 33	     16	  0.00%
 34	     15	  0.00%
 35	     24	  0.00%
 36	     29	  0.00%
 37	     27	  0.00%
 38	     32	  0.00%
 39	     62	  0.00%
 40	     76	  0.00%
 41	    103	  0.00%
 42	    162	  0.00%
 43	    209	  0.01%
 44	    458	  0.01%
 45	    496	  0.01%
 46	    693	  0.02%
 47	   1189	  0.03%
 48	   2037	  0.05%
 49	   4584	  0.11%
 50	  12789	  0.32%
 51	  72181	  1.80%
 52	3904345	 97.62%
3999604 reads passed initial QC


criterion=sequence-density
sequence-density=0.18
sequence-density-rank=1
fanout-score=6.56
fanout-score-rank=5
prefix-density=0.78
prefix-fanout=1.5
sequence=ACGTGCTTAATACGTGCTTAA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=23
fanout-score=18.70
fanout-score-rank=1
prefix-density=0.31
prefix-fanout=1.3
sequence=CATTTTATTTCTATCTTTCCATACATAACCAAAAAATGGAAAAGATTCTTCAAGAGTCTCGGGTCCTAGAAGTGCATGATAAATACCGCCAAAGCCCAATACTGCAGAGGAAATTAAGTGAAGTACGCCAGATACAAAGTATGGAAAGGTGTCTATGACTTCCCCGCCAGGACCCACCCCCCAACCTAGAGTAGCTAGGTGGGGAAGTAAAATTAATCCTTGTTCATACATTGGCTTCTCCGGTACGAAATGGGCCACTTCAAATAGGTTCATTGCTCCGGCCCAGAATACGATTAATCCAGCATGGGCTACATGAGCTCCCAGCAG
                                 Started job on |	Feb 12 13:14:26
                             Started mapping on |	Feb 12 13:14:26
                                    Finished on |	Feb 12 13:14:32
       Mapping speed, Million of reads per hour |	2399.76

                          Number of input reads |	3999604
                      Average input read length |	51
                                    UNIQUE READS:
                   Uniquely mapped reads number |	3140550
                        Uniquely mapped reads % |	78.52%
                          Average mapped length |	51.72
                       Number of splices: Total |	298263
            Number of splices: Annotated (sjdb) |	293656
                       Number of splices: GT/AG |	291726
                       Number of splices: GC/AG |	5282
                       Number of splices: AT/AC |	766
               Number of splices: Non-canonical |	489
                      Mismatch rate per base, % |	0.40%
                         Deletion rate per base |	0.01%
                        Deletion average length |	2.40
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.38
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	678519
             % of reads mapped to multiple loci |	16.96%
        Number of reads mapped to too many loci |	104653
             % of reads mapped to too many loci |	2.62%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.89%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	180535	180535	180535
N_multimapping	678519	678519	678519
N_noFeature	406644	3093871	442833
N_ambiguous	19957	154	9329
UnstrandedReadsAssigned:2713949 PositiveStrandReadsAssigned:46525 NegativeStrandReadsAssigned:2688388
Dataset is classified negative stranded
MeadianReadLen=52 20thPercentileLength=52 echo kmer=47
SRR5423441 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 52,400
[index] number of k-mers: 62,057,036
[index] number of equivalence classes: 130,681
[quant] running in single-end mode
[quant] will process file 1: SRR5423441-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,999,604 reads, 3,250,868 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,366 rounds

  52401 SRR5423441.ke.tsv
  34699 SRR5423441.se.tsv
  87100 total
==> SRR5423441.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Potri.005G200100.1.v4.1	2018	1919	153	21.7188
Potri.005G024800.1.v4.1	1035	936	23.1022	6.72351
Potri.004G059700.1.v4.1	961	862	1	0.316018
Potri.007G009000.2.v4.1	1416	1317	0	0
Potri.003G141000.2.v4.1	2943	2844	58.3898	5.59277
Potri.016G087400.1.v4.1	270	171	12	19.1163
Potri.015G069301.1.v4.1	564	465	0	0
Potri.010G195200.1.v4.1	1773	1674	3.71574	0.604656
Potri.012G127500.1.v4.1	977	878	5	1.5513

==> SRR5423441.se.tsv <==
Potri.001G166300.v4.1	0
Potri.001G448400.v4.1	1
Potri.001G233950.v4.1	0
Potri.001G122700.v4.1	29
Potri.001G212900.v4.1	0
Potri.001G182400.v4.1	0
Potri.001G256600.v4.1	0
Potri.001G040500.v4.1	1
Potri.001G416900.v4.1	0
Potri.001G452600.v4.1	0
SRR5423441 completed mapping pipeline successfully
