Starting /dee2/code/volunteer_pipeline.sh SRR5423442 current disk space = 3051190681600 free memory = 1582719016 SRR5423442 SRAfilesize ed02be838e69d91f4b2b351d954bab9b SRR5423442.sra SRR5423442.sra file validated SRR5423442 is single end SRR5423442 is conventional basespace SRR5423442 read1 length is 52 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5423442_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.129 34.0 31.0 34.0 30.0 34.0 2 32.176 34.0 31.0 34.0 30.0 34.0 3 32.329 34.0 31.0 34.0 30.0 34.0 4 35.66575 37.0 35.0 37.0 33.0 37.0 5 35.71525 37.0 35.0 37.0 33.0 37.0 6 35.672 37.0 35.0 37.0 33.0 37.0 7 35.75375 37.0 35.0 37.0 33.0 37.0 8 35.78125 37.0 35.0 37.0 33.0 37.0 9 37.3655 39.0 37.0 39.0 34.0 39.0 10 37.36875 39.0 37.0 39.0 34.0 39.0 11 37.185 39.0 37.0 39.0 33.0 39.0 12 37.1975 39.0 37.0 39.0 33.0 39.0 13 37.28325 39.0 37.0 39.0 34.0 39.0 14 38.13075 40.0 37.0 41.0 33.0 41.0 15 38.51275 40.0 38.0 41.0 34.0 41.0 16 38.593 40.0 38.0 41.0 34.0 41.0 17 38.29275 40.0 38.0 41.0 33.0 41.0 18 38.4245 40.0 38.0 41.0 33.0 41.0 19 38.51225 40.0 38.0 41.0 34.0 41.0 20 38.518 40.0 38.0 41.0 34.0 41.0 21 38.447 40.0 38.0 41.0 34.0 41.0 22 38.531 40.0 38.0 41.0 34.0 41.0 23 38.5075 40.0 38.0 41.0 34.0 41.0 24 38.46375 40.0 38.0 41.0 34.0 41.0 25 38.43725 40.0 38.0 41.0 34.0 41.0 26 38.38575 40.0 38.0 41.0 34.0 41.0 27 38.196 40.0 38.0 41.0 33.0 41.0 28 38.21375 40.0 38.0 41.0 34.0 41.0 29 38.16875 40.0 38.0 41.0 33.0 41.0 30 37.84525 40.0 37.0 41.0 32.0 41.0 31 38.03125 40.0 38.0 41.0 33.0 41.0 32 37.733 40.0 37.0 41.0 32.0 41.0 33 37.96375 40.0 38.0 41.0 33.0 41.0 34 37.93925 40.0 37.0 41.0 33.0 41.0 35 37.91475 40.0 37.0 41.0 33.0 41.0 36 37.9325 40.0 37.0 41.0 33.0 41.0 37 37.87125 40.0 37.0 41.0 33.0 41.0 38 37.72825 40.0 37.0 41.0 32.0 41.0 39 37.61075 40.0 37.0 41.0 31.0 41.0 40 37.642 40.0 37.0 41.0 32.0 41.0 41 37.736 40.0 37.0 41.0 33.0 41.0 42 37.632 40.0 37.0 41.0 32.0 41.0 43 37.331 40.0 37.0 41.0 31.0 41.0 44 37.25 40.0 37.0 41.0 31.0 41.0 45 37.199 40.0 37.0 41.0 30.0 41.0 46 37.28575 40.0 36.0 41.0 31.0 41.0 47 37.1655 39.0 36.0 41.0 31.0 41.0 48 37.07175 39.0 36.0 41.0 31.0 41.0 49 37.001 39.0 36.0 41.0 30.0 41.0 50 36.94625 39.0 36.0 41.0 30.0 41.0 51 36.7885 39.0 35.0 41.0 30.0 41.0 52 35.8565 38.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2209 1 0.0 2209 2 0.0 2209 3 0.0 2209 4 0.0 2209 5 0.0 2209 6 0.0 2209 7 0.0 2209 8 0.0 2209 9 0.0 2209 10 0.0 2209 11 0.0 2209 12 0.0 2209 13 0.0 2209 14 0.0 2209 15 0.0 2209 16 0.0 2209 17 0.0 2209 18 0.0 2209 19 0.0 2209 20 0.0 2209 21 0.0 2209 22 0.0 2209 23 0.0 2209 24 0.0 2209 25 0.0 2209 26 0.0 2209 27 0.0 2209 28 0.0 2209 29 0.0 2209 30 0.0 2209 31 0.0 2209 32 0.0 2209 33 0.0 2209 34 0.0 2209 35 0.0 2209 36 0.0 2209 37 0.0 2209 38 0.0 2209 39 0.0 2209 40 0.0 2209 41 0.0 2209 42 0.0 2209 43 0.0 2209 44 0.0 2209 45 0.0 2209 46 0.0 2209 47 0.0 2209 48 0.0 2209 49 0.0 2209 50 0.0 2209 51 0.0 2209 52 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 1.0 21 0.0 22 2.0 23 3.0 24 5.0 25 14.0 26 19.0 27 39.0 28 34.0 29 36.0 30 74.0 31 84.0 32 110.0 33 131.0 34 175.0 35 230.0 36 290.0 37 450.0 38 758.0 39 1537.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.37956935403104 9.864797195793692 6.43465197796695 47.32098147220831 2 22.275 14.249999999999998 35.725 27.750000000000004 3 20.849999999999998 18.325 22.8 38.025 4 26.55 25.05 20.95 27.450000000000003 5 24.0 31.15 24.075 20.775 6 19.375 32.05 24.775 23.799999999999997 7 16.3 22.55 40.575 20.575 8 18.0 22.45 30.25 29.299999999999997 9 17.7 21.325 33.85 27.125 10 19.1 37.325 23.875 19.7 11 23.125 27.55 21.224999999999998 28.1 12 20.8 23.974999999999998 27.35 27.875 13 19.825 27.425 27.275 25.474999999999998 14 20.474999999999998 27.025 28.125 24.375 15 19.525000000000002 25.525 28.975 25.974999999999998 16 20.575 28.325 25.924999999999997 25.174999999999997 17 20.849999999999998 27.375 27.3 24.474999999999998 18 20.225 27.500000000000004 26.974999999999998 25.3 19 21.175 27.975 25.900000000000002 24.95 20 21.2 26.474999999999998 26.775 25.55 21 19.85992996498249 27.113556778389196 26.3631815907954 26.663331665832917 22 20.05 28.65 24.825 26.474999999999998 23 20.5 27.025 26.125 26.35 24 20.849999999999998 26.125 25.974999999999998 27.05 25 21.2 28.075 26.075 24.65 26 21.825 26.474999999999998 26.650000000000002 25.05 27 20.95 26.35 25.95 26.75 28 21.025 27.650000000000002 26.974999999999998 24.349999999999998 29 21.05 26.8 27.625 24.525 30 21.85 25.85 26.8 25.5 31 20.225 28.799999999999997 26.6 24.375 32 21.075 26.35 26.224999999999998 26.35 33 20.225 26.1 27.750000000000004 25.924999999999997 34 21.875 25.674999999999997 26.625 25.825 35 22.1 26.724999999999998 25.674999999999997 25.5 36 21.525 25.775 25.124999999999996 27.575 37 19.85 26.450000000000003 26.174999999999997 27.525 38 22.275 25.624999999999996 25.95 26.150000000000002 39 20.599999999999998 24.25 27.025 28.125 40 21.15 26.325 26.3 26.224999999999998 41 21.575 26.224999999999998 25.874999999999996 26.325 42 21.099999999999998 24.05 26.5 28.349999999999998 43 20.549999999999997 26.6 26.275 26.575 44 22.6 27.35 26.575 23.474999999999998 45 23.375 24.425 25.45 26.75 46 21.925 27.750000000000004 25.124999999999996 25.2 47 23.35 27.275 25.0 24.375 48 22.97872340425532 25.682102628285357 23.979974968710888 27.359198998748436 49 20.925 26.75 24.8 27.525 50 22.725 27.35 24.125 25.8 51 20.66033016508254 26.563281640820406 25.41270635317659 27.363681840920464 52 22.425 26.700000000000003 24.625 26.25 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.0 16 1.0 17 1.0 18 1.0 19 1.0 20 4.0 21 7.0 22 12.5 23 18.0 24 13.5 25 9.0 26 12.5 27 16.0 28 22.5 29 29.0 30 35.0 31 41.0 32 55.5 33 70.0 34 92.5 35 115.0 36 137.5 37 160.0 38 195.0 39 239.5 40 249.0 41 254.0 42 259.0 43 295.0 44 331.0 45 335.0 46 339.0 47 327.0 48 315.0 49 303.0 50 291.0 51 321.5 52 352.0 53 338.0 54 324.0 55 281.0 56 238.0 57 201.0 58 164.0 59 153.0 60 142.0 61 133.0 62 124.0 63 94.5 64 55.0 65 45.0 66 34.5 67 24.0 68 21.5 69 19.0 70 14.0 71 9.0 72 7.0 73 5.0 74 3.5 75 2.0 76 1.5 77 1.0 78 1.5 79 2.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.05 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.125 49 0.0 50 0.0 51 0.05 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.9 #Duplication Level Percentage of deduplicated Percentage of total 1 93.56017997750281 83.175 2 4.133858267716536 7.35 3 1.0123734533183353 2.7 4 0.562429696287964 2.0 5 0.22497187851518563 1.0 6 0.1687289088863892 0.8999999999999999 7 0.0843644544431946 0.525 8 0.028121484814398204 0.2 9 0.0843644544431946 0.675 >10 0.140607424071991 1.4749999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGC 14 0.35000000000000003 No Hit CCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACT 12 0.3 No Hit GTCCCTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAAC 11 0.27499999999999997 No Hit GGGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCG 11 0.27499999999999997 No Hit GGGTAAGCTACATAAGCAATAAATTGATTTTCTTCTCCAGCAACGGGCTCGA 11 0.27499999999999997 No Hit GTCGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAAT 9 0.22499999999999998 No Hit CTCATTACGAGCTTGTACACATGCTTCTAGAGCTACTCGATTAGCAACGGCA 9 0.22499999999999998 No Hit GTCCTAGAAGTGCATGATAAATACCGCCAAAGCCCAATACTGCAGAGGAAAT 9 0.22499999999999998 No Hit CTCCAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACT 8 0.2 No Hit GTCCGCGACCCCTGGATGCCGAAGGCGTCCTTGGGGCGATCTCGTAGTTCCT 7 0.17500000000000002 No Hit CTCTTGACCAAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTTTCC 7 0.17500000000000002 No Hit CCTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTGGCCCGCAGCAGT 7 0.17500000000000002 No Hit GGGCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGAT 6 0.15 No Hit CGCAGAAGTAGGAATAATGGCACCAGAAATGATATTGTTTCCATAAAGTAGA 6 0.15 No Hit CTCGTATTTTCCCCTCTGCCTCGGGTTGTCTCGCGATACCTTCTACAGCTTG 6 0.15 No Hit GCCCGTCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTAC 6 0.15 No Hit CCAGCAACGGGCTCGATGTCGTAGCATCGTCCCTTATAACGATCAAGACTGG 6 0.15 No Hit GCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGCCCGC 6 0.15 No Hit CCCTACGCCACACACATGACGGTTTACGTGCTTAATGACCGCATGTGCAGGT 5 0.125 No Hit CCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACTGCGGCCCCT 5 0.125 No Hit GATCAAACTAGAGGTTACCAAAGAACCATGCATAGCACTGAATAGGGAGCCG 5 0.125 No Hit GTCCCTTATAACGATCAAGACTGGTAAGCCCGTCGGTCCACACAGTTGTCCA 5 0.125 No Hit CTTGACCAAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTTTCCCT 5 0.125 No Hit CTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGCCC 5 0.125 No Hit CTGGAATACAATCATAAAGTTAAAAGTACCAGAGATTCCTAGAGGCATCCCA 5 0.125 No Hit GCTCCATTTGCTAGCTTCACGGATAATTTCATTACCCTCACGAGCAAGATCA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra Read 200000 spots for SRR5423442.sra Written 200000 spots for SRR5423442.sra SRR ids: ['SRR5423442.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_xmwce565 SRR5423442.sra spots: 4000000 blocks: [[1, 200000], [200001, 400000], [400001, 600000], [600001, 800000], [800001, 1000000], [1000001, 1200000], [1200001, 1400000], [1400001, 1600000], [1600001, 1800000], [1800001, 2000000], [2000001, 2200000], [2200001, 2400000], [2400001, 2600000], [2600001, 2800000], [2800001, 3000000], [3000001, 3200000], [3200001, 3400000], [3400001, 3600000], [3600001, 3800000], [3800001, 4000000]] SRR5423442 file size 703953 SRR5423442 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423442 SRR5423442_1.fastq Input file: SRR5423442_1.fastq trimmed: SRR5423442-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Wed Feb 12 13:24:27 2025 >> started Wed Feb 12 13:24:29 2025 >> done (1.907s) 4000000 reads processed; of these: 154 ( 0.00%) short reads filtered out after trimming by size control 207 ( 0.01%) empty reads filtered out after trimming by size control 3999639 (99.99%) reads available; of these: 79193 ( 1.98%) trimmed reads available after processing 3920446 (98.02%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 11 0.00% 19 9 0.00% 20 5 0.00% 21 0 0.00% 22 0 0.00% 23 1 0.00% 24 1 0.00% 25 4 0.00% 26 1 0.00% 27 3 0.00% 28 3 0.00% 29 3 0.00% 30 4 0.00% 31 5 0.00% 32 3 0.00% 33 16 0.00% 34 10 0.00% 35 13 0.00% 36 18 0.00% 37 37 0.00% 38 33 0.00% 39 50 0.00% 40 51 0.00% 41 92 0.00% 42 125 0.00% 43 130 0.00% 44 259 0.01% 45 343 0.01% 46 494 0.01% 47 780 0.02% 48 1460 0.04% 49 3574 0.09% 50 10374 0.26% 51 61281 1.53% 52 3920446 98.02% 3999639 reads passed initial QC criterion=sequence-density sequence-density=0.18 sequence-density-rank=1 fanout-score=6.57 fanout-score-rank=7 prefix-density=0.79 prefix-fanout=1.5 sequence=ACGTGCTTAATACGTGCTTAA criterion=fanout-score sequence-density=0.02 sequence-density-rank=24 fanout-score=20.22 fanout-score-rank=1 prefix-density=0.31 prefix-fanout=1.2 sequence=CATTTTATTTCTATCTTTCCATACATAACCAAAAAATGGAAAAGATTCTTCAAGAGTCTCGGGTCCTAGAAGTGCATGATAAATACCGCCAAAGCCCAATACTGCAGAGGAAATTAAGTGAAGTACGCCAGATACAAAGTATGGAAAGGTGTCTATGACTTCCCCGCCAGGACCCACCCCCCAACCTAGAGTAGCTAGGTGGGGAAGTAAAATTAATCCTTGTTCATACATTGGCTTCTCCGGTACGAAATGGGCCACTTCAAATAGGTTCATTGCTCCGGCCCAGAATACGATTAATCCAGCATGGGCTACATGAGCTCCCAGCAGTT Started job on | Feb 12 13:24:42 Started mapping on | Feb 12 13:24:42 Finished on | Feb 12 13:24:48 Mapping speed, Million of reads per hour | 2399.78 Number of input reads | 3999639 Average input read length | 51 UNIQUE READS: Uniquely mapped reads number | 3141939 Uniquely mapped reads % | 78.56% Average mapped length | 51.74 Number of splices: Total | 298665 Number of splices: Annotated (sjdb) | 293977 Number of splices: GT/AG | 292085 Number of splices: GC/AG | 5239 Number of splices: AT/AC | 831 Number of splices: Non-canonical | 510 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.01% Deletion average length | 2.35 Insertion rate per base | 0.00% Insertion average length | 1.37 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 677311 % of reads mapped to multiple loci | 16.93% Number of reads mapped to too many loci | 106382 % of reads mapped to too many loci | 2.66% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.84% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 180389 180389 180389 N_multimapping 677311 677311 677311 N_noFeature 407685 3095005 444241 N_ambiguous 19836 125 9347 UnstrandedReadsAssigned:2714418 PositiveStrandReadsAssigned:46809 NegativeStrandReadsAssigned:2688351 Dataset is classified negative stranded MeadianReadLen=52 20thPercentileLength=52 echo kmer=47 SRR5423442 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR5423442-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,999,639 reads, 3,241,880 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,219 rounds 52401 SRR5423442.ke.tsv 34699 SRR5423442.se.tsv 87100 total ==> SRR5423442.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 151 21.4191 Potri.005G024800.1.v4.1 1035 936 18 5.23475 Potri.004G059700.1.v4.1 961 862 1 0.315786 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 65.3222 6.25217 Potri.016G087400.1.v4.1 270 171 11 17.5104 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 1 0.162609 Potri.012G127500.1.v4.1 977 878 4 1.24012 ==> SRR5423442.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 0 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 36 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR5423442 completed mapping pipeline successfully