Starting /dee2/code/volunteer_pipeline.sh SRR5423537 current disk space = 3051842883584 free memory = 1576996732 SRR5423537 SRAfilesize f541a8c3c9256c0f1b12a99d8cceda06 SRR5423537.sra SRR5423537.sra file validated SRR5423537 is single end SRR5423537 is conventional basespace SRR5423537 read1 length is 52 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5423537_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2855 34.0 31.0 34.0 30.0 34.0 2 32.475 34.0 31.0 34.0 31.0 34.0 3 32.549 34.0 31.0 34.0 30.0 34.0 4 35.96525 37.0 35.0 37.0 35.0 37.0 5 35.70075 37.0 35.0 37.0 33.0 37.0 6 35.90375 37.0 35.0 37.0 35.0 37.0 7 35.968 37.0 35.0 37.0 35.0 37.0 8 36.01175 37.0 35.0 37.0 35.0 37.0 9 37.8245 39.0 38.0 39.0 35.0 39.0 10 37.63475 39.0 37.0 39.0 35.0 39.0 11 37.7465 39.0 38.0 39.0 35.0 39.0 12 37.707 39.0 38.0 39.0 35.0 39.0 13 37.6685 39.0 37.0 39.0 35.0 39.0 14 39.0735 40.0 38.0 41.0 36.0 41.0 15 38.985 40.0 38.0 41.0 35.0 41.0 16 39.00725 40.0 38.0 41.0 36.0 41.0 17 38.99675 40.0 38.0 41.0 36.0 41.0 18 38.862 40.0 38.0 41.0 36.0 41.0 19 38.943 40.0 38.0 41.0 35.0 41.0 20 38.89275 40.0 38.0 41.0 35.0 41.0 21 38.89075 40.0 39.0 41.0 35.0 41.0 22 38.839 40.0 38.0 41.0 35.0 41.0 23 38.82975 40.0 38.0 41.0 35.0 41.0 24 38.77375 40.0 38.0 41.0 34.0 41.0 25 38.666 40.0 38.0 41.0 35.0 41.0 26 38.75925 40.0 38.0 41.0 35.0 41.0 27 38.719 40.0 38.0 41.0 35.0 41.0 28 38.61425 40.0 38.0 41.0 34.0 41.0 29 38.46375 40.0 38.0 41.0 34.0 41.0 30 38.389 40.0 38.0 41.0 33.0 41.0 31 38.38925 40.0 38.0 41.0 34.0 41.0 32 38.5015 40.0 38.0 41.0 34.0 41.0 33 38.3835 40.0 38.0 41.0 34.0 41.0 34 38.332 40.0 38.0 41.0 34.0 41.0 35 38.24575 40.0 38.0 41.0 34.0 41.0 36 38.2265 40.0 38.0 41.0 33.0 41.0 37 38.253 40.0 38.0 41.0 34.0 41.0 38 38.04375 40.0 38.0 41.0 33.0 41.0 39 37.79275 40.0 37.0 41.0 32.0 41.0 40 37.77775 40.0 37.0 41.0 33.0 41.0 41 37.76575 40.0 37.0 41.0 33.0 41.0 42 37.5835 40.0 37.0 41.0 32.0 41.0 43 37.401 40.0 37.0 41.0 32.0 41.0 44 37.29925 40.0 37.0 41.0 32.0 41.0 45 37.11575 40.0 36.0 41.0 31.0 41.0 46 37.00525 39.0 36.0 41.0 31.0 41.0 47 36.9885 39.0 36.0 41.0 31.0 41.0 48 36.833 39.0 36.0 41.0 31.0 41.0 49 36.72075 39.0 35.0 41.0 30.0 41.0 50 36.5165 39.0 35.0 41.0 30.0 41.0 51 36.4345 39.0 35.0 41.0 30.0 41.0 52 35.16925 38.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1207 1 0.0 1207 2 0.0 1207 3 0.0 1207 4 0.0 1207 5 0.0 1207 6 0.0 1207 7 0.0 1207 8 0.0 1207 9 0.0 1207 10 0.0 1207 11 0.0 1207 12 0.0 1207 13 0.0 1207 14 0.0 1207 15 0.0 1207 16 0.0 1207 17 0.0 1207 18 0.0 1207 19 0.0 1207 20 0.0 1207 21 0.0 1207 22 0.0 1207 23 0.0 1207 24 0.0 1207 25 0.0 1207 26 0.0 1207 27 0.0 1207 28 0.0 1207 29 0.0 1207 30 0.0 1207 31 0.0 1207 32 0.0 1207 33 0.0 1207 34 0.0 1207 35 0.0 1207 36 0.0 1207 37 0.0 1207 38 0.0 1207 39 0.0 1207 40 0.0 1207 41 0.0 1207 42 0.0 1207 43 0.0 1207 44 0.0 1207 45 0.0 1207 46 0.0 1207 47 0.0 1207 48 0.0 1207 49 0.0 1207 50 0.0 1207 51 0.0 1207 52 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 0.0 14 0.0 15 1.0 16 1.0 17 0.0 18 1.0 19 2.0 20 3.0 21 3.0 22 7.0 23 5.0 24 11.0 25 16.0 26 18.0 27 21.0 28 28.0 29 40.0 30 50.0 31 69.0 32 77.0 33 90.0 34 138.0 35 203.0 36 293.0 37 425.0 38 775.0 39 1716.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.45922208281054 12.823086574654956 5.972396486825596 38.745294855708906 2 22.0 15.9 35.6 26.5 3 19.85 18.05 27.025 35.075 4 24.95 28.925 20.549999999999997 25.575 5 23.1 31.8 22.775000000000002 22.325 6 20.875 30.575000000000003 23.724999999999998 24.825 7 16.075 19.400000000000002 40.6 23.925 8 19.375 17.849999999999998 30.925000000000004 31.85 9 19.325 18.0 32.85 29.825000000000003 10 20.849999999999998 34.300000000000004 24.224999999999998 20.625 11 26.8 23.425 18.975 30.8 12 24.775 18.15 25.0 32.074999999999996 13 22.35 22.725 27.474999999999998 27.450000000000003 14 22.325 23.225 27.200000000000003 27.250000000000004 15 22.675 23.7 25.6 28.025 16 23.549999999999997 22.675 25.374999999999996 28.4 17 23.400000000000002 23.599999999999998 25.624999999999996 27.375 18 22.7 22.725 25.324999999999996 29.25 19 22.75 24.275 26.8 26.174999999999997 20 24.05 24.2 24.075 27.675 21 22.2 23.200000000000003 26.525 28.075 22 23.575 22.625 25.174999999999997 28.625 23 23.200000000000003 22.55 25.3 28.95 24 22.95 24.075 24.325 28.65 25 22.75 22.85 27.025 27.375 26 22.0 23.599999999999998 26.0 28.4 27 21.099999999999998 22.75 27.6 28.549999999999997 28 24.2 23.45 24.975 27.375 29 22.975 22.95 26.075 28.000000000000004 30 23.375 22.375 25.624999999999996 28.625 31 22.2 22.525000000000002 26.8 28.475 32 24.474999999999998 22.275 25.55 27.700000000000003 33 23.025000000000002 22.925 25.874999999999996 28.175 34 22.75 23.3 26.375 27.575 35 24.425 22.525000000000002 26.224999999999998 26.825 36 24.0 22.8 24.7 28.499999999999996 37 22.5 24.15 24.474999999999998 28.875 38 22.325 22.325 25.575 29.775000000000002 39 23.3 23.325000000000003 25.75 27.625 40 24.725 23.225 24.425 27.625 41 24.325 23.875 23.724999999999998 28.075 42 23.075000000000003 22.75 25.6 28.575 43 24.975 22.675 24.85 27.500000000000004 44 23.474999999999998 22.675 25.900000000000002 27.950000000000003 45 23.599999999999998 22.175 24.975 29.25 46 23.125 22.175 26.125 28.575 47 23.175 22.900000000000002 26.650000000000002 27.275 48 23.525 23.65 23.849999999999998 28.975 49 24.075 21.725 25.15 29.049999999999997 50 23.875 22.275 25.124999999999996 28.725 51 22.15 21.125 26.0 30.725 52 23.325000000000003 22.725 24.825 29.125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.0 16 0.5 17 0.0 18 1.0 19 2.0 20 2.5 21 3.0 22 3.0 23 3.0 24 4.0 25 5.0 26 7.0 27 9.0 28 9.0 29 9.0 30 12.5 31 16.0 32 25.5 33 35.0 34 40.0 35 45.0 36 66.5 37 88.0 38 98.0 39 122.5 40 137.0 41 157.0 42 177.0 43 213.0 44 249.0 45 268.0 46 287.0 47 306.5 48 326.0 49 328.0 50 330.0 51 357.5 52 385.0 53 379.5 54 374.0 55 383.5 56 393.0 57 355.0 58 317.0 59 261.0 60 205.0 61 175.5 62 146.0 63 118.0 64 85.5 65 81.0 66 61.5 67 42.0 68 39.0 69 36.0 70 35.5 71 35.0 72 33.0 73 31.0 74 23.5 75 16.0 76 11.0 77 6.0 78 7.0 79 8.0 80 5.5 81 3.0 82 2.5 83 2.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.325 #Duplication Level Percentage of deduplicated Percentage of total 1 92.18794225870364 81.425 2 4.840079252759694 8.55 3 1.8397962071893574 4.875 4 0.5660911406736484 2.0 5 0.1415227851684121 0.625 6 0.19813189923577695 1.05 7 0.16982734220209456 1.05 8 0.02830455703368242 0.2 9 0.02830455703368242 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGGCAATCGGACGGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGACTTA 9 0.22499999999999998 No Hit GTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCG 8 0.2 No Hit ATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGCTATAGAC 7 0.17500000000000002 No Hit GTCGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAAT 7 0.17500000000000002 No Hit GGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTT 7 0.17500000000000002 No Hit GTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCA 7 0.17500000000000002 No Hit GATAGAACTCGCACCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC 7 0.17500000000000002 No Hit CTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGGCCCCCG 7 0.17500000000000002 No Hit GTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGC 6 0.15 No Hit CTAAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAGCTAGTTAGCAGGCTGA 6 0.15 No Hit CTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGCCC 6 0.15 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCTCT 6 0.15 No Hit CCAGAACCCAAAAACTTTGATTTCTCATAAGGTGCTGGCGGAGTCCTAAAAG 6 0.15 No Hit GTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGC 6 0.15 No Hit CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTC 6 0.15 No Hit GCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTT 5 0.125 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTCCT 5 0.125 No Hit CCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCACC 5 0.125 No Hit GCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGCCCGC 5 0.125 No Hit CGATAGAACTCGCACCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.025 0.0 0.0 0.0 0.0 24 0.025 0.0 0.0 0.0 0.0 25 0.025 0.0 0.0 0.0 0.0 26 0.025 0.0 0.0 0.0 0.0 27 0.025 0.0 0.0 0.0 0.0 28 0.025 0.0 0.0 0.0 0.0 29 0.025 0.0 0.0 0.0 0.0 30 0.025 0.0 0.0 0.0 0.0 31 0.025 0.0 0.0 0.0 0.0 32 0.025 0.0 0.0 0.0 0.0 33 0.025 0.0 0.0 0.0 0.0 34 0.025 0.0 0.0 0.0 0.0 35 0.025 0.0 0.0 0.0 0.0 36 0.025 0.0 0.0 0.0 0.0 37 0.025 0.0 0.0 0.0 0.0 38 0.025 0.0 0.0 0.0 0.0 39 0.025 0.0 0.0 0.0 0.0 40 0.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra Read 200000 spots for SRR5423537.sra Written 200000 spots for SRR5423537.sra SRR ids: ['SRR5423537.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_a6fb8h4j SRR5423537.sra spots: 4000000 blocks: [[1, 200000], [200001, 400000], [400001, 600000], [600001, 800000], [800001, 1000000], [1000001, 1200000], [1200001, 1400000], [1400001, 1600000], [1600001, 1800000], [1800001, 2000000], [2000001, 2200000], [2200001, 2400000], [2400001, 2600000], [2600001, 2800000], [2800001, 3000000], [3000001, 3200000], [3200001, 3400000], [3400001, 3600000], [3600001, 3800000], [3800001, 4000000]] SRR5423537 file size 703957 SRR5423537 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423537 SRR5423537_1.fastq Input file: SRR5423537_1.fastq trimmed: SRR5423537-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Wed Feb 12 17:12:15 2025 >> started Wed Feb 12 17:12:17 2025 >> done (1.984s) 4000000 reads processed; of these: 298 ( 0.01%) short reads filtered out after trimming by size control 179 ( 0.00%) empty reads filtered out after trimming by size control 3999523 (99.99%) reads available; of these: 93294 ( 2.33%) trimmed reads available after processing 3906229 (97.67%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 7 0.00% 19 9 0.00% 20 9 0.00% 21 0 0.00% 22 1 0.00% 23 6 0.00% 24 10 0.00% 25 32 0.00% 26 26 0.00% 27 42 0.00% 28 62 0.00% 29 56 0.00% 30 52 0.00% 31 93 0.00% 32 138 0.00% 33 161 0.00% 34 119 0.00% 35 118 0.00% 36 141 0.00% 37 164 0.00% 38 228 0.01% 39 221 0.01% 40 282 0.01% 41 375 0.01% 42 413 0.01% 43 538 0.01% 44 758 0.02% 45 1489 0.04% 46 1900 0.05% 47 2065 0.05% 48 2666 0.07% 49 5452 0.14% 50 11631 0.29% 51 64030 1.60% 52 3906229 97.67% 3999523 reads passed initial QC criterion=sequence-density sequence-density=0.61 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=33 prefix-density=0.59 prefix-fanout=2.0 sequence=CCGTCAATTCCTTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=19.81 fanout-score-rank=1 prefix-density=0.20 prefix-fanout=1.0 sequence=GCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAATTCCGAAGGTCTAAAGGATCGATAGGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGT Started job on | Feb 12 17:12:28 Started mapping on | Feb 12 17:12:28 Finished on | Feb 12 17:12:36 Mapping speed, Million of reads per hour | 1799.79 Number of input reads | 3999523 Average input read length | 51 UNIQUE READS: Uniquely mapped reads number | 2710844 Uniquely mapped reads % | 67.78% Average mapped length | 51.82 Number of splices: Total | 317761 Number of splices: Annotated (sjdb) | 313761 Number of splices: GT/AG | 311097 Number of splices: GC/AG | 6089 Number of splices: AT/AC | 206 Number of splices: Non-canonical | 369 Mismatch rate per base, % | 0.43% Deletion rate per base | 0.00% Deletion average length | 1.55 Insertion rate per base | 0.00% Insertion average length | 1.18 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 274414 % of reads mapped to multiple loci | 6.86% Number of reads mapped to too many loci | 996393 % of reads mapped to too many loci | 24.91% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 0.44% % of reads unmapped: other | 0.00% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1014265 1014265 1014265 N_multimapping 274414 274414 274414 N_noFeature 408592 2645551 423306 N_ambiguous 61353 16 10766 UnstrandedReadsAssigned:2240899 PositiveStrandReadsAssigned:65277 NegativeStrandReadsAssigned:2276772 Dataset is classified negative stranded MeadianReadLen=52 20thPercentileLength=52 echo kmer=47 SRR5423537 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR5423537-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,999,523 reads, 2,972,248 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,019 rounds 52401 SRR5423537.ke.tsv 34699 SRR5423537.se.tsv 87100 total ==> SRR5423537.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 58 10.0991 Potri.005G024800.1.v4.1 1035 936 15.0205 5.36213 Potri.004G059700.1.v4.1 961 862 9 3.4887 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 36.3172 4.26688 Potri.016G087400.1.v4.1 270 171 63 123.104 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 0 0 Potri.012G127500.1.v4.1 977 878 26 9.89479 ==> SRR5423537.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 4 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 40 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR5423537 completed mapping pipeline successfully