Starting /dee2/code/volunteer_pipeline.sh SRR5423541 current disk space = 3051952644096 free memory = 1443275852 SRR5423541 SRAfilesize bc393b91d7572037d46429a725050fb6 SRR5423541.sra SRR5423541.sra file validated SRR5423541 is single end SRR5423541 is conventional basespace SRR5423541 read1 length is 52 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5423541_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 52 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68325 34.0 31.0 34.0 31.0 34.0 2 32.79575 34.0 31.0 34.0 31.0 34.0 3 32.86825 34.0 31.0 34.0 31.0 34.0 4 36.12275 37.0 37.0 37.0 35.0 37.0 5 36.16425 37.0 37.0 37.0 35.0 37.0 6 36.232 37.0 37.0 37.0 35.0 37.0 7 36.1705 37.0 37.0 37.0 35.0 37.0 8 36.2245 37.0 37.0 37.0 35.0 37.0 9 37.92125 39.0 38.0 39.0 35.0 39.0 10 37.89075 39.0 38.0 39.0 35.0 39.0 11 37.98325 39.0 38.0 39.0 35.0 39.0 12 37.973 39.0 38.0 39.0 35.0 39.0 13 37.7875 39.0 38.0 39.0 35.0 39.0 14 39.35875 41.0 39.0 41.0 36.0 41.0 15 39.25975 41.0 39.0 41.0 36.0 41.0 16 39.20825 41.0 39.0 41.0 36.0 41.0 17 39.22 41.0 39.0 41.0 36.0 41.0 18 39.1225 40.0 39.0 41.0 36.0 41.0 19 39.192 40.0 39.0 41.0 36.0 41.0 20 39.21975 40.0 39.0 41.0 36.0 41.0 21 39.156 40.0 39.0 41.0 36.0 41.0 22 39.17825 40.0 39.0 41.0 36.0 41.0 23 39.084 40.0 39.0 41.0 36.0 41.0 24 39.0605 40.0 39.0 41.0 36.0 41.0 25 39.077 40.0 39.0 41.0 36.0 41.0 26 38.97175 40.0 39.0 41.0 36.0 41.0 27 38.779 40.0 39.0 41.0 35.0 41.0 28 38.758 40.0 39.0 41.0 35.0 41.0 29 38.64075 40.0 38.0 41.0 35.0 41.0 30 38.54825 40.0 38.0 41.0 34.0 41.0 31 38.41125 40.0 38.0 41.0 34.0 41.0 32 38.36275 40.0 38.0 41.0 34.0 41.0 33 38.22625 40.0 38.0 41.0 33.0 41.0 34 38.16525 40.0 38.0 41.0 33.0 41.0 35 37.969 40.0 38.0 41.0 33.0 41.0 36 37.9695 40.0 38.0 41.0 33.0 41.0 37 37.986 40.0 38.0 41.0 33.0 41.0 38 37.9305 40.0 38.0 41.0 33.0 41.0 39 37.69825 40.0 38.0 41.0 32.0 41.0 40 37.5225 40.0 38.0 41.0 31.0 41.0 41 37.32025 40.0 37.0 41.0 31.0 41.0 42 37.238 40.0 37.0 41.0 30.0 41.0 43 37.1435 40.0 37.0 41.0 31.0 41.0 44 37.13125 40.0 37.0 41.0 31.0 41.0 45 36.9425 40.0 36.0 41.0 30.0 41.0 46 36.88125 40.0 36.0 41.0 30.0 41.0 47 36.79525 40.0 36.0 41.0 30.0 41.0 48 36.71375 40.0 36.0 41.0 30.0 41.0 49 36.5355 39.0 36.0 41.0 29.0 41.0 50 36.522 39.0 35.0 41.0 29.0 41.0 51 36.382 39.0 35.0 41.0 29.0 41.0 52 34.8485 38.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2203 1 0.0 2203 2 0.0 2203 3 0.0 2203 4 0.0 2203 5 0.0 2203 6 0.0 2203 7 0.0 2203 8 0.0 2203 9 0.0 2203 10 0.0 2203 11 0.0 2203 12 0.0 2203 13 0.0 2203 14 0.0 2203 15 0.0 2203 16 0.0 2203 17 0.0 2203 18 0.0 2203 19 0.0 2203 20 0.0 2203 21 0.0 2203 22 0.0 2203 23 0.0 2203 24 0.0 2203 25 0.0 2203 26 0.0 2203 27 0.0 2203 28 0.0 2203 29 0.0 2203 30 0.0 2203 31 0.0 2203 32 0.0 2203 33 0.0 2203 34 0.0 2203 35 0.0 2203 36 0.0 2203 37 0.0 2203 38 0.0 2203 39 0.0 2203 40 0.0 2203 41 0.0 2203 42 0.0 2203 43 0.0 2203 44 0.0 2203 45 0.0 2203 46 0.0 2203 47 0.0 2203 48 0.0 2203 49 0.0 2203 50 0.0 2203 51 0.0 2203 52 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 1.0 14 2.0 15 0.0 16 2.0 17 1.0 18 2.0 19 3.0 20 3.0 21 1.0 22 9.0 23 8.0 24 12.0 25 19.0 26 23.0 27 22.0 28 35.0 29 37.0 30 55.0 31 60.0 32 61.0 33 108.0 34 110.0 35 166.0 36 263.0 37 361.0 38 753.0 39 1870.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.7625250501002 12.049098196392785 5.61122244488978 38.57715430861723 2 21.975 15.575 35.125 27.325 3 19.900000000000002 18.775 26.3 35.025 4 24.7 27.900000000000002 21.875 25.525 5 24.525 31.35 22.675 21.45 6 20.95 31.5 23.925 23.625 7 16.6 19.7 40.925 22.775000000000002 8 19.2 18.25 30.325000000000003 32.225 9 20.45 18.925 31.4 29.225 10 20.65 34.375 23.150000000000002 21.825 11 26.424999999999997 23.075000000000003 19.775000000000002 30.725 12 24.6 20.075000000000003 24.625 30.7 13 21.425 23.875 27.55 27.150000000000002 14 22.225 22.975 27.0 27.800000000000004 15 23.025000000000002 22.675 25.95 28.349999999999998 16 23.45 22.15 25.424999999999997 28.975 17 22.225 23.400000000000002 26.674999999999997 27.700000000000003 18 22.6 23.275000000000002 26.325 27.800000000000004 19 23.175 25.05 24.95 26.825 20 22.975 22.95 26.6 27.474999999999998 21 22.35 23.275000000000002 25.900000000000002 28.475 22 23.225 23.5 25.55 27.725 23 23.400000000000002 23.125 24.825 28.65 24 22.7 22.75 26.1 28.449999999999996 25 24.05 22.25 25.2 28.499999999999996 26 21.925 23.775 26.150000000000002 28.15 27 22.175 23.150000000000002 26.174999999999997 28.499999999999996 28 23.150000000000002 23.474999999999998 25.35 28.025 29 22.975 23.849999999999998 25.724999999999998 27.450000000000003 30 23.025000000000002 23.225 25.775 27.975 31 23.875 22.650000000000002 25.3 28.175 32 23.875 22.5 26.474999999999998 27.150000000000002 33 21.925 23.674999999999997 24.975 29.425 34 22.0 23.724999999999998 26.35 27.925 35 23.599999999999998 21.975 25.4 29.025000000000002 36 23.75 22.525000000000002 24.224999999999998 29.5 37 22.575 23.45 25.224999999999998 28.749999999999996 38 23.674999999999997 23.7 24.425 28.199999999999996 39 22.95 23.549999999999997 24.349999999999998 29.15 40 23.674999999999997 23.5 24.125 28.7 41 24.575 23.075000000000003 25.474999999999998 26.875 42 23.25 22.725 25.85 28.175 43 25.15 22.575 25.775 26.5 44 24.125 22.825 25.724999999999998 27.325 45 23.95 22.2 25.374999999999996 28.475 46 22.85 23.5 25.775 27.875 47 24.75 21.85 24.275 29.125 48 23.225 22.675 25.1 28.999999999999996 49 23.45 22.35 24.4 29.799999999999997 50 22.525000000000002 23.025000000000002 25.6 28.849999999999998 51 22.25 22.900000000000002 25.15 29.7 52 23.9 22.925 23.925 29.25 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 2.0 20 1.5 21 1.0 22 1.0 23 1.0 24 2.5 25 4.0 26 5.0 27 6.0 28 10.5 29 15.0 30 20.5 31 26.0 32 27.5 33 29.0 34 37.5 35 46.0 36 61.0 37 76.0 38 88.0 39 117.5 40 135.0 41 171.0 42 207.0 43 218.5 44 230.0 45 254.5 46 279.0 47 318.0 48 357.0 49 348.0 50 339.0 51 366.0 52 393.0 53 397.0 54 401.0 55 375.5 56 350.0 57 321.0 58 292.0 59 259.5 60 227.0 61 185.0 62 143.0 63 114.0 64 84.5 65 84.0 66 57.5 67 31.0 68 38.0 69 45.0 70 33.0 71 21.0 72 23.5 73 26.0 74 22.0 75 18.0 76 14.5 77 11.0 78 9.5 79 8.0 80 7.5 81 7.0 82 4.0 83 1.0 84 1.0 85 1.0 86 1.5 87 2.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.9 #Duplication Level Percentage of deduplicated Percentage of total 1 93.35372636262514 83.92500000000001 2 4.171301446051167 7.5 3 1.418242491657397 3.8249999999999997 4 0.5005561735261401 1.7999999999999998 5 0.25027808676307006 1.125 6 0.1946607341490545 1.05 7 0.08342602892102337 0.525 8 0.0 0.0 9 0.0 0.0 >10 0.027808676307007785 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGGCAATCGGACGGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGACTTA 10 0.25 No Hit CTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGCCC 7 0.17500000000000002 No Hit CCAGAACCCAAAAACTTTGATTTCTCATAAGGTGCTGGCGGAGTCCTAAAAG 7 0.17500000000000002 No Hit AAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGCCCGCGCG 7 0.17500000000000002 No Hit CTAAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAGCTAGTTAGCAGGCTGA 6 0.15 No Hit CGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTG 6 0.15 No Hit CTCAAACTTCCTTGGCCTGGAAGGCCATAGTCCCTCTAAGAAGCTGGCCGCG 6 0.15 No Hit CAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCACCG 6 0.15 No Hit GCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGACTTCCCTTGCCTA 6 0.15 No Hit GATAGAACTCGCACCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC 6 0.15 No Hit CGATAGAACTCGCACCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAA 6 0.15 No Hit ATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGCTATAGAC 5 0.125 No Hit CTCCAATGGATCCTCGTTAAGGGATTTAGATTGTACTCATTCCAATTACCAG 5 0.125 No Hit CCGGAATCGAACCCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCTCT 5 0.125 No Hit GTGGTATTTCACCTTCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGTCA 5 0.125 No Hit CACTCATCTTGGGGTGGGCTTACTACTTAGATGCTTTCAGCAGTTATCCGCT 5 0.125 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAACCCAAA 5 0.125 No Hit GTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGC 5 0.125 No Hit CTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGGCCCCCG 5 0.125 No Hit GTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGGCCCCCGAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.025 0.0 0.0 0.0 0.0 29 0.025 0.0 0.0 0.0 0.0 30 0.025 0.0 0.0 0.0 0.0 31 0.025 0.0 0.0 0.0 0.0 32 0.025 0.0 0.0 0.0 0.0 33 0.025 0.0 0.0 0.0 0.0 34 0.025 0.0 0.0 0.0 0.0 35 0.025 0.0 0.0 0.0 0.0 36 0.025 0.0 0.0 0.0 0.0 37 0.025 0.0 0.0 0.0 0.0 38 0.025 0.0 0.0 0.0 0.0 39 0.025 0.0 0.0 0.0 0.0 40 0.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra Read 200000 spots for SRR5423541.sra Written 200000 spots for SRR5423541.sra SRR ids: ['SRR5423541.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_cuxuakpu SRR5423541.sra spots: 4000000 blocks: [[1, 200000], [200001, 400000], [400001, 600000], [600001, 800000], [800001, 1000000], [1000001, 1200000], [1200001, 1400000], [1400001, 1600000], [1600001, 1800000], [1800001, 2000000], [2000001, 2200000], [2200001, 2400000], [2400001, 2600000], [2600001, 2800000], [2800001, 3000000], [3000001, 3200000], [3200001, 3400000], [3400001, 3600000], [3600001, 3800000], [3800001, 4000000]] SRR5423541 file size 703984 SRR5423541 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5423541 SRR5423541_1.fastq Input file: SRR5423541_1.fastq trimmed: SRR5423541-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Wed Feb 12 16:39:11 2025 >> started Wed Feb 12 16:39:13 2025 >> done (2.778s) 4000000 reads processed; of these: 283 ( 0.01%) short reads filtered out after trimming by size control 153 ( 0.00%) empty reads filtered out after trimming by size control 3999564 (99.99%) reads available; of these: 109550 ( 2.74%) trimmed reads available after processing 3890014 (97.26%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 15 0.00% 19 17 0.00% 20 15 0.00% 21 1 0.00% 22 7 0.00% 23 15 0.00% 24 25 0.00% 25 30 0.00% 26 40 0.00% 27 47 0.00% 28 68 0.00% 29 90 0.00% 30 109 0.00% 31 144 0.00% 32 227 0.01% 33 252 0.01% 34 199 0.00% 35 186 0.00% 36 253 0.01% 37 350 0.01% 38 384 0.01% 39 391 0.01% 40 633 0.02% 41 791 0.02% 42 740 0.02% 43 974 0.02% 44 1280 0.03% 45 2398 0.06% 46 3108 0.08% 47 3272 0.08% 48 4051 0.10% 49 7106 0.18% 50 14012 0.35% 51 68320 1.71% 52 3890014 97.26% 3999564 reads passed initial QC criterion=sequence-density sequence-density=0.58 sequence-density-rank=1 fanout-score=1.92 fanout-score-rank=36 prefix-density=0.56 prefix-fanout=1.9 sequence=CCGTCAATTCCTTT criterion=fanout-score sequence-density=0.02 sequence-density-rank=29 fanout-score=16.61 fanout-score-rank=1 prefix-density=0.21 prefix-fanout=2.0 sequence=CCCGAAGGGGGCCTTCTTCGCCTTCCACCTAAGCTGCGCAGGAAAGGCCCAAAGCCAATCCCAGGGAACAGTGAAGCTTCATAGGGTCTTTCTGTCCAGGTGCAGGTAGTCCGCATCTTCACAGACATGTCTATTTCACCGAGCCTCTCTCCGAGACAGTGCCCAGATCGTTACGCCTTTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACC Started job on | Feb 12 16:39:28 Started mapping on | Feb 12 16:39:28 Finished on | Feb 12 16:39:35 Mapping speed, Million of reads per hour | 2056.92 Number of input reads | 3999564 Average input read length | 51 UNIQUE READS: Uniquely mapped reads number | 2717724 Uniquely mapped reads % | 67.95% Average mapped length | 51.82 Number of splices: Total | 319101 Number of splices: Annotated (sjdb) | 315140 Number of splices: GT/AG | 312366 Number of splices: GC/AG | 6219 Number of splices: AT/AC | 190 Number of splices: Non-canonical | 326 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.00% Deletion average length | 1.54 Insertion rate per base | 0.00% Insertion average length | 1.19 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 275306 % of reads mapped to multiple loci | 6.88% Number of reads mapped to too many loci | 983461 % of reads mapped to too many loci | 24.59% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 0.57% % of reads unmapped: other | 0.00% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1006534 1006534 1006534 N_multimapping 275306 275306 275306 N_noFeature 406734 2652103 421807 N_ambiguous 61388 24 10825 UnstrandedReadsAssigned:2249602 PositiveStrandReadsAssigned:65597 NegativeStrandReadsAssigned:2285092 Dataset is classified negative stranded MeadianReadLen=52 20thPercentileLength=52 echo kmer=47 SRR5423541 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in single-end mode [quant] will process file 1: SRR5423541-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,999,564 reads, 2,968,085 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,155 rounds 52401 SRR5423541.ke.tsv 34699 SRR5423541.se.tsv 87100 total ==> SRR5423541.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1919 50 8.69718 Potri.005G024800.1.v4.1 1035 936 9 3.20959 Potri.004G059700.1.v4.1 961 862 12 4.64683 Potri.007G009000.2.v4.1 1416 1317 0 0 Potri.003G141000.2.v4.1 2943 2844 30.5277 3.58301 Potri.016G087400.1.v4.1 270 171 76 148.355 Potri.015G069301.1.v4.1 564 465 0 0 Potri.010G195200.1.v4.1 1773 1674 2 0.398802 Potri.012G127500.1.v4.1 977 878 23 8.74413 ==> SRR5423541.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 1 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 29 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 2 SRR5423541 completed mapping pipeline successfully