Starting /dee2/code/volunteer_pipeline.sh SRR5487668 current disk space = 3050288746496 free memory = 1579273188 SRR5487668 SRAfilesize 065be819f5997e4953f0808a7403b94a SRR5487668.sra SRR5487668.sra file validated SRR5487668 is paired end SRR5487668 is conventional basespace SRR5487668 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5487668_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.35825 34.0 33.0 34.0 33.0 34.0 2 33.58925 34.0 34.0 34.0 33.0 34.0 3 33.317 34.0 34.0 34.0 33.0 34.0 4 33.4575 34.0 34.0 34.0 33.0 34.0 5 33.56975 34.0 34.0 34.0 33.0 34.0 6 37.317 38.0 38.0 38.0 36.0 38.0 7 37.49625 38.0 38.0 38.0 37.0 38.0 8 37.513 38.0 38.0 38.0 38.0 38.0 9 37.36525 38.0 38.0 38.0 37.0 38.0 10-14 37.348850000000006 38.0 38.0 38.0 37.0 38.0 15-19 37.43585 38.0 38.0 38.0 37.6 38.0 20-24 37.59525 38.0 38.0 38.0 38.0 38.0 25-29 37.5318 38.0 38.0 38.0 38.0 38.0 30-34 37.470749999999995 38.0 38.0 38.0 38.0 38.0 35-39 37.50475 38.0 38.0 38.0 37.8 38.0 40-44 37.38355 38.0 38.0 38.0 37.0 38.0 45-49 37.4457 38.0 38.0 38.0 37.8 38.0 50-54 37.40675 38.0 38.0 38.0 37.2 38.0 55-59 37.30715 38.0 38.0 38.0 37.0 38.0 60-64 37.32015 38.0 38.0 38.0 37.0 38.0 65-69 37.31045 38.0 38.0 38.0 37.0 38.0 70-74 37.30839999999999 38.0 38.0 38.0 37.0 38.0 75-79 37.19295 38.0 38.0 38.0 37.0 38.0 80-84 37.12935 38.0 38.0 38.0 36.8 38.0 85-89 36.97185 38.0 38.0 38.0 36.2 38.0 90-94 37.08729999999999 38.0 38.0 38.0 36.6 38.0 95-99 37.09105 38.0 38.0 38.0 36.8 38.0 100-104 36.96345 38.0 38.0 38.0 36.2 38.0 105-109 36.938849999999995 38.0 38.0 38.0 36.0 38.0 110-114 37.0167 38.0 38.0 38.0 36.0 38.0 115-119 36.80735 38.0 38.0 38.0 35.4 38.0 120-124 36.4872 38.0 38.0 38.0 34.8 38.0 125-129 36.497699999999995 38.0 38.0 38.0 34.6 38.0 130-134 36.34775 38.0 38.0 38.0 34.0 38.0 135-139 36.443200000000004 38.0 38.0 38.0 34.8 38.0 140-144 36.107000000000006 38.0 38.0 38.0 33.6 38.0 145-149 35.991499999999995 38.0 38.0 38.0 33.8 38.0 150 31.5465 36.0 32.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 2.0 11 0.0 12 0.0 13 3.0 14 2.0 15 1.0 16 1.0 17 3.0 18 3.0 19 8.0 20 3.0 21 1.0 22 3.0 23 5.0 24 4.0 25 6.0 26 16.0 27 13.0 28 10.0 29 18.0 30 32.0 31 31.0 32 43.0 33 57.0 34 69.0 35 126.0 36 305.0 37 3234.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.07035175879397 10.376884422110551 9.195979899497488 36.35678391959799 2 25.3 13.450000000000001 32.0 29.25 3 21.85 18.8 25.474999999999998 33.875 4 25.074999999999996 26.125 21.25 27.55 5 23.724999999999998 30.825000000000003 24.15 21.3 6 21.0 32.75 24.525 21.725 7 15.65 22.475 41.525 20.349999999999998 8 19.225 22.25 30.925000000000004 27.6 9 19.45 21.275 32.025 27.250000000000004 10-14 22.75 27.0 24.44 25.81 15-19 22.63 24.610000000000003 26.279999999999998 26.479999999999997 20-24 21.765 25.564999999999998 26.025 26.645000000000003 25-29 21.485000000000003 25.055 26.855 26.605 30-34 21.349999999999998 25.779999999999998 26.21 26.66 35-39 21.985 24.955 25.874999999999996 27.185 40-44 23.345 24.529999999999998 26.125 26.0 45-49 22.2 24.69 26.045 27.065 50-54 21.709999999999997 25.779999999999998 25.915 26.595000000000002 55-59 21.715 25.324999999999996 26.02 26.939999999999998 60-64 21.845 24.685000000000002 26.96 26.51 65-69 21.345 26.174999999999997 25.669999999999998 26.810000000000002 70-74 22.375 25.94 25.56 26.125 75-79 21.895 25.295 25.790000000000003 27.02 80-84 21.445 25.564999999999998 25.335 27.655 85-89 22.045 25.074999999999996 26.25 26.63 90-94 22.055 25.44 25.924999999999997 26.58 95-99 21.875 25.424999999999997 25.83 26.87 100-104 22.814999999999998 24.985 26.064999999999998 26.135 105-109 22.509999999999998 25.380000000000003 26.205000000000002 25.905 110-114 22.1 25.695 25.900000000000002 26.305 115-119 22.095000000000002 24.97 25.674999999999997 27.26 120-124 23.0 26.484999999999996 25.03 25.485000000000003 125-129 22.17 25.645 24.57 27.615000000000002 130-134 22.685 25.205 25.290000000000003 26.82 135-139 22.5 25.895000000000003 25.09 26.515 140-144 23.41 25.990000000000002 24.825 25.775 145-149 23.215 25.900000000000002 24.51 26.375 150 23.225 28.15 23.5 25.124999999999996 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.5 15 0.5 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 1.0 25 2.0 26 4.0 27 6.5 28 7.0 29 10.5 30 14.0 31 17.0 32 22.5 33 32.0 34 39.5 35 55.0 36 79.5 37 88.0 38 91.5 39 99.5 40 105.5 41 119.0 42 111.5 43 107.0 44 134.0 45 153.0 46 170.5 47 191.5 48 196.5 49 186.0 50 180.5 51 164.5 52 150.5 53 164.0 54 166.5 55 134.5 56 126.0 57 127.5 58 110.5 59 110.0 60 97.5 61 81.5 62 85.0 63 69.0 64 35.0 65 16.0 66 11.0 67 18.5 68 21.0 69 16.5 70 14.0 71 8.0 72 9.5 73 14.5 74 10.0 75 6.5 76 4.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.4 #Duplication Level Percentage of deduplicated Percentage of total 1 87.74875621890547 70.55 2 7.462686567164178 12.0 3 2.300995024875622 5.55 4 1.1194029850746268 3.5999999999999996 5 0.5286069651741294 2.125 6 0.21766169154228857 1.05 7 0.18656716417910446 1.05 8 0.03109452736318408 0.2 9 0.06218905472636816 0.44999999999999996 >10 0.3420398009950249 3.4250000000000003 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCAC 20 0.5 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 16 0.4 No Hit CGGCAATCGGACGGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGACT 14 0.35000000000000003 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAACCCA 14 0.35000000000000003 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 12 0.3 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGGAATCTCGTATGC 11 0.27499999999999997 TruSeq Adapter, Index 6 (97% over 36bp) CTCCGATTCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTATTTCAC 10 0.25 No Hit GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG 10 0.25 No Hit GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT 10 0.25 No Hit GTCGGCAATCGGACGGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGA 10 0.25 No Hit CCAGAACCCAAAAACTTTGATTTCTCATAAGGTGCTGGCGGAGTCCTAAA 10 0.25 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 9 0.22499999999999998 No Hit GTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCAT 9 0.22499999999999998 No Hit GCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGG 8 0.2 No Hit CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCC 7 0.17500000000000002 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 7 0.17500000000000002 No Hit GTCAGTATCGCTGCGGGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGC 7 0.17500000000000002 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 7 0.17500000000000002 No Hit GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGGACTTCTCGCG 7 0.17500000000000002 No Hit GCCTTGCGACCATACTCCCCCCAGAACCCAAAAACTTTGATTTCTCATAA 7 0.17500000000000002 No Hit GTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTC 6 0.15 No Hit CCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAAT 6 0.15 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 6 0.15 No Hit GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT 6 0.15 No Hit GGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTCTTTCGC 6 0.15 No Hit GTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTA 6 0.15 No Hit GCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTA 6 0.15 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 5 0.125 No Hit GTCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCC 5 0.125 No Hit CGATAGAACTCGCACCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGG 5 0.125 No Hit CTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCAACAGGGTCTTC 5 0.125 No Hit GATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCA 5 0.125 No Hit CCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCAT 5 0.125 No Hit GCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCC 5 0.125 No Hit CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGT 5 0.125 No Hit GTCATATCTAGTATTCAGAGTTTGCCTCGATTTGGTACCGCTCTCGCGGC 5 0.125 No Hit CCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGA 5 0.125 No Hit GCCGAAGCTCCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGG 5 0.125 No Hit CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTT 5 0.125 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 5 0.125 No Hit GCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGC 5 0.125 No Hit TGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATTT 5 0.125 No Hit GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG 5 0.125 No Hit GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.0625 0.0 0.0 0.0 0.0 68-69 0.0875 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.16249999999999998 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.3125 0.0 0.0 0.0 0.0 88-89 0.4375 0.0 0.0 0.0 0.0 90-91 0.525 0.0 0.0 0.0 0.0 92-93 0.55 0.0 0.0 0.0 0.0 94-95 0.625 0.0 0.0 0.0 0.0 96-97 0.7875000000000001 0.0 0.0 0.0 0.0 98-99 0.8625 0.0 0.0 0.0 0.0 100-101 1.0625 0.0 0.0 0.0 0.0 102-103 1.2374999999999998 0.0 0.0 0.0 0.0 104-105 1.4500000000000002 0.0 0.0 0.0 0.0 106-107 1.7125 0.0 0.0 0.0 0.0 108-109 1.8875 0.0 0.0 0.0 0.0 110-111 2.3 0.0 0.0 0.0 0.0 112-113 2.675 0.0 0.0 0.0 0.0 114-115 2.9125 0.0 0.0 0.0 0.0 116-117 3.175 0.0 0.0 0.0 0.0 118-119 3.5375 0.0 0.0 0.0 0.0 120-121 3.85 0.0 0.0 0.0 0.0 122-123 4.0125 0.0 0.0 0.0 0.0 124-125 4.4875 0.0 0.0 0.0 0.0 126-127 4.9375 0.0 0.0 0.0 0.0 128-129 5.375 0.0 0.0 0.0 0.0 130-131 5.8125 0.0 0.0 0.0 0.0 132-133 6.525 0.0 0.0 0.0 0.0 134-135 7.2 0.0 0.0 0.0 0.0 136-137 7.6625 0.0 0.0 0.0 0.0 138 8.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR5487668 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR5487668_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.60475 33.0 33.0 34.0 32.0 34.0 2 32.86975 34.0 33.0 34.0 32.0 34.0 3 32.96575 34.0 33.0 34.0 32.0 34.0 4 32.81625 34.0 33.0 34.0 32.0 34.0 5 32.83175 34.0 33.0 34.0 32.0 34.0 6 36.895 38.0 38.0 38.0 36.0 38.0 7 36.953 38.0 38.0 38.0 36.0 38.0 8 36.95875 38.0 38.0 38.0 36.0 38.0 9 36.95525 38.0 38.0 38.0 36.0 38.0 10-14 36.7762 38.0 38.0 38.0 35.6 38.0 15-19 36.91435 38.0 38.0 38.0 36.2 38.0 20-24 36.9126 38.0 38.0 38.0 36.0 38.0 25-29 36.966499999999996 38.0 38.0 38.0 36.8 38.0 30-34 36.71665 38.0 38.0 38.0 36.4 38.0 35-39 35.78775 38.0 38.0 38.0 33.2 38.0 40-44 35.93345 38.0 38.0 38.0 35.0 38.0 45-49 35.18920000000001 38.0 36.8 38.0 29.4 38.0 50-54 36.60215 38.0 38.0 38.0 35.2 38.0 55-59 36.894600000000004 38.0 38.0 38.0 36.8 38.0 60-64 36.95215 38.0 38.0 38.0 36.6 38.0 65-69 36.8513 38.0 38.0 38.0 36.2 38.0 70-74 36.7978 38.0 38.0 38.0 36.2 38.0 75-79 36.7604 38.0 38.0 38.0 36.2 38.0 80-84 36.2669 38.0 38.0 38.0 35.2 38.0 85-89 34.783550000000005 38.0 38.0 38.0 28.8 38.0 90-94 33.45285 38.0 34.4 38.0 21.8 38.0 95-99 35.839200000000005 38.0 38.0 38.0 32.0 38.0 100-104 36.2697 38.0 38.0 38.0 34.4 38.0 105-109 36.397800000000004 38.0 38.0 38.0 35.0 38.0 110-114 36.22525 38.0 38.0 38.0 34.4 38.0 115-119 36.0826 38.0 38.0 38.0 34.0 38.0 120-124 36.04775 38.0 38.0 38.0 34.0 38.0 125-129 35.762 38.0 38.0 38.0 33.6 38.0 130-134 34.9602 38.0 38.0 38.0 30.4 38.0 135-139 33.830450000000006 38.0 36.8 38.0 19.0 38.0 140-144 31.04715 37.0 28.6 38.0 4.2 38.0 145-149 32.786199999999994 38.0 35.0 38.0 4.2 38.0 150 27.94475 35.0 23.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 4.0 4 3.0 5 3.0 6 2.0 7 2.0 8 3.0 9 5.0 10 2.0 11 3.0 12 4.0 13 4.0 14 1.0 15 7.0 16 9.0 17 11.0 18 7.0 19 7.0 20 8.0 21 7.0 22 20.0 23 30.0 24 33.0 25 29.0 26 28.0 27 30.0 28 35.0 29 39.0 30 49.0 31 61.0 32 72.0 33 72.0 34 123.0 35 177.0 36 485.0 37 2619.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.84731290808639 20.51732797589151 10.170768458061277 25.464590657960823 2 32.25 22.425 28.050000000000004 17.275 3 24.4 24.575 29.349999999999998 21.675 4 25.25 33.550000000000004 21.175 20.025000000000002 5 29.975 32.775 19.1 18.15 6 24.425 36.35 20.1 19.125 7 22.95 21.5 34.150000000000006 21.4 8 24.275 23.474999999999998 25.624999999999996 26.625 9 24.625 23.35 27.3 24.725 10-14 25.765 27.139999999999997 23.815 23.28 15-19 26.505000000000003 25.955000000000002 25.34 22.2 20-24 26.540000000000003 26.700000000000003 24.675 22.085 25-29 27.229999999999997 26.855 24.525 21.39 30-34 26.263492383738523 26.34923837385252 25.2244527388278 22.162816503581155 35-39 26.17139334155364 26.885532264693797 24.404027949034116 22.539046444718455 40-44 26.43512315778625 26.6000206121818 24.363598886942185 22.601257343089767 45-49 25.999281646056748 25.999281646056748 25.17830571091385 22.82313099697265 50-54 26.423233184531274 26.48342278176255 25.07398304659678 22.019360987109398 55-59 26.025 26.21 25.905 21.86 60-64 27.12 25.790000000000003 25.36 21.73 65-69 27.1 25.825 25.005 22.07 70-74 26.255 26.145000000000003 24.935 22.665 75-79 26.58 25.759999999999998 24.83 22.830000000000002 80-84 26.50742349257651 25.684274315725688 25.603474396525606 22.204827795172204 85-89 25.83561067899937 26.103636745848224 25.46773176371663 22.59302081143578 90-94 26.2116147900564 26.316064340923333 25.433465636097765 22.0388552329225 95-99 26.47666045621633 26.884535978649478 24.532957349312653 22.105846215821543 100-104 26.545 25.895000000000003 25.89 21.67 105-109 26.314999999999998 26.369999999999997 25.779999999999998 21.535 110-114 27.12 27.560000000000002 23.985 21.335 115-119 27.495000000000005 26.365 24.404999999999998 21.735 120-124 27.79 26.919999999999998 23.945 21.345 125-129 27.72645109459731 27.073709580236994 24.035950994175536 21.163888330990158 130-134 27.556963318688055 26.448349851844284 24.461019720036784 21.53366710943088 135-139 27.560873215785055 25.61922753988245 25.052476910159534 21.767422334172963 140-144 27.867095391211144 25.878885316184352 24.05680600214362 22.19721329046088 145-149 27.883584375797803 27.01046719428134 23.829461322440643 21.276487107480214 150 27.5887943971986 26.613306653326664 25.46273136568284 20.335167583791897 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.5 22 2.0 23 4.0 24 3.5 25 1.5 26 3.0 27 7.0 28 11.5 29 14.5 30 18.5 31 22.5 32 26.5 33 33.0 34 47.0 35 57.0 36 76.5 37 86.0 38 82.5 39 113.0 40 132.5 41 122.0 42 129.0 43 144.0 44 151.0 45 162.5 46 165.5 47 204.0 48 227.0 49 194.0 50 167.0 51 144.5 52 133.5 53 135.5 54 132.0 55 119.5 56 107.5 57 106.5 58 96.0 59 89.5 60 89.0 61 74.5 62 88.5 63 84.5 64 41.0 65 18.5 66 15.0 67 17.0 68 15.0 69 11.0 70 13.0 71 15.0 72 10.0 73 7.0 74 9.0 75 6.5 76 4.0 77 3.0 78 2.5 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.44999999999999996 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.8699999999999999 35-39 2.68 40-44 2.97 45-49 2.555 50-54 0.315 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.9900000000000001 85-89 4.859999999999999 90-94 4.26 95-99 0.705 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.42 130-134 2.13 135-139 4.72 140-144 6.7 145-149 2.075 150 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.89999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 88.66103739445114 73.5 2 7.32810615199035 12.15 3 2.0205066344993967 5.025 4 1.0253317249698433 3.4000000000000004 5 0.4523522316043426 1.875 6 0.0 0.0 7 0.24125452352231602 1.4000000000000001 8 0.09047044632086852 0.6 9 0.0 0.0 >10 0.18094089264173704 2.0500000000000003 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCAAGG 25 0.625 No Hit GGAGTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCA 13 0.325 No Hit ACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 13 0.325 No Hit CTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTT 11 0.27499999999999997 No Hit CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 10 0.25 No Hit AGGAGTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGC 10 0.25 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 8 0.2 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 8 0.2 Illumina Single End PCR Primer 1 (100% over 50bp) GGCGGGACTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAA 8 0.2 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 7 0.17500000000000002 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 7 0.17500000000000002 No Hit CTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTT 7 0.17500000000000002 No Hit GTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATAC 7 0.17500000000000002 No Hit GGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAA 7 0.17500000000000002 No Hit AGTCAACGGGCGAGTAAACCCGTAAGGCGCAAGGAAGCTGACTGGCGGGA 7 0.17500000000000002 No Hit GCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGAGGCC 7 0.17500000000000002 No Hit AAGGAGTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCG 7 0.17500000000000002 No Hit GGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGG 5 0.125 No Hit GAGACAGGTTAGTTTTACCCTACTGATGACAGTGTCGCAATAGTAATCCA 5 0.125 No Hit GTCAGGCGGGACTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAA 5 0.125 No Hit CTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGAC 5 0.125 No Hit CAGAAATCTCGTGTGGAACAAAAGGGTAAAAGCTCGTTTGATTCTGATTT 5 0.125 No Hit ACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCT 5 0.125 No Hit GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCAC 5 0.125 No Hit GTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTG 5 0.125 No Hit GGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTC 5 0.125 No Hit GGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCT 5 0.125 No Hit GTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCAAGGAAGCTGACTGG 5 0.125 No Hit CTCGAGGGTTGCACCGCCGACCGACCTTGATCTTCTGAGAAGGGTTCGAG 5 0.125 No Hit AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG 5 0.125 No Hit GTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTTCTAA 5 0.125 No Hit AGTAAACCCGTAAGGCGCAAGGAAGCTGACTGGCGGGATCCCCTCGAGGG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.0625 0.0 0.0 0.0 0.0 68-69 0.0875 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.175 0.0 0.0 0.0 0.0 84-85 0.2 0.0 0.0 0.0 0.0 86-87 0.2625 0.0 0.0 0.0 0.0 88-89 0.3375 0.0 0.0 0.0 0.0 90-91 0.42500000000000004 0.0 0.0 0.0 0.0 92-93 0.45 0.0 0.0 0.0 0.0 94-95 0.55 0.0 0.0 0.0 0.0 96-97 0.7 0.0 0.0 0.0 0.0 98-99 0.7749999999999999 0.0 0.0 0.0 0.0 100-101 0.9625 0.0 0.0 0.0 0.0 102-103 1.1375000000000002 0.0 0.0 0.0 0.0 104-105 1.325 0.0 0.0 0.0 0.0 106-107 1.5625 0.0 0.0 0.0 0.0 108-109 1.7375 0.0 0.0 0.0 0.0 110-111 2.1125 0.0 0.0 0.0 0.0 112-113 2.5 0.0 0.0 0.0 0.0 114-115 2.7375 0.0 0.0 0.0 0.0 116-117 2.9875 0.0 0.0 0.0 0.0 118-119 3.325 0.0 0.0 0.0 0.0 120-121 3.6125 0.0 0.0 0.0 0.0 122-123 3.7750000000000004 0.0 0.0 0.0 0.0 124-125 4.2125 0.0 0.0 0.0 0.0 126-127 4.637499999999999 0.0 0.0 0.0 0.0 128-129 5.074999999999999 0.0 0.0 0.0 0.0 130-131 5.4625 0.0 0.0 0.0 0.0 132-133 6.05 0.0 0.0 0.0 0.0 134-135 6.6 0.0 0.0 0.0 0.0 136-137 6.975 0.0 0.0 0.0 0.0 138 7.35 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGGGAG 10 0.007552131 140.21251 5 >>END_MODULE Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934628 spots for SRR5487668.sra Written 934628 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra Read 934613 spots for SRR5487668.sra Written 934613 spots for SRR5487668.sra SRR ids: ['SRR5487668.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_8vuur9bq SRR5487668.sra spots: 18692275 blocks: [[1, 934613], [934614, 1869226], [1869227, 2803839], [2803840, 3738452], [3738453, 4673065], [4673066, 5607678], [5607679, 6542291], [6542292, 7476904], [7476905, 8411517], [8411518, 9346130], [9346131, 10280743], [10280744, 11215356], [11215357, 12149969], [12149970, 13084582], [13084583, 14019195], [14019196, 14953808], [14953809, 15888421], [15888422, 16823034], [16823035, 17757647], [17757648, 18692275]] SRR5487668 file size 6275989 SRR5487668 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR5487668 SRR5487668_1.fastq SRR5487668_2.fastq Input file: SRR5487668_1.fastq Paired file: SRR5487668_2.fastq trimmed: SRR5487668-trimmed-pair1.fastq, SRR5487668-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 06:18:56 2025 >> started Wed Feb 12 06:19:16 2025 >> done (20.070s) 18692275 read pairs processed; of these: 27268 ( 0.15%) short read pairs filtered out after trimming by size control 71400 ( 0.38%) empty read pairs filtered out after trimming by size control 18593607 (99.47%) read pairs available; of these: 6169754 (33.18%) trimmed read pairs available after processing 12423853 (66.82%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 4 0.00% 20 3 0.00% 21 2 0.00% 22 9 0.00% 23 10 0.00% 24 11 0.00% 25 15 0.00% 26 6 0.00% 27 12 0.00% 28 5 0.00% 29 8 0.00% 30 9 0.00% 31 10 0.00% 32 15 0.00% 33 14 0.00% 34 16 0.00% 35 22 0.00% 36 17 0.00% 37 32 0.00% 38 30 0.00% 39 27 0.00% 40 33 0.00% 41 38 0.00% 42 45 0.00% 43 38 0.00% 44 43 0.00% 45 65 0.00% 46 69 0.00% 47 78 0.00% 48 102 0.00% 49 107 0.00% 50 124 0.00% 51 121 0.00% 52 143 0.00% 53 133 0.00% 54 164 0.00% 55 170 0.00% 56 204 0.00% 57 216 0.00% 58 256 0.00% 59 304 0.00% 60 358 0.00% 61 376 0.00% 62 427 0.00% 63 522 0.00% 64 541 0.00% 65 656 0.00% 66 818 0.00% 67 1238 0.01% 68 2814 0.02% 69 7044 0.04% 70 5475 0.03% 71 2696 0.01% 72 2129 0.01% 73 1971 0.01% 74 2008 0.01% 75 2123 0.01% 76 2054 0.01% 77 2314 0.01% 78 2376 0.01% 79 2701 0.01% 80 2974 0.02% 81 3429 0.02% 82 3967 0.02% 83 4716 0.03% 84 6965 0.04% 85 7625 0.04% 86 7815 0.04% 87 7885 0.04% 88 8775 0.05% 89 8514 0.05% 90 9233 0.05% 91 10372 0.06% 92 11631 0.06% 93 13324 0.07% 94 13478 0.07% 95 15125 0.08% 96 15665 0.08% 97 16339 0.09% 98 16604 0.09% 99 17282 0.09% 100 17902 0.10% 101 19323 0.10% 102 20172 0.11% 103 21978 0.12% 104 27139 0.15% 105 24411 0.13% 106 26550 0.14% 107 26855 0.14% 108 26751 0.14% 109 27289 0.15% 110 28667 0.15% 111 30724 0.17% 112 33682 0.18% 113 36421 0.20% 114 37095 0.20% 115 40372 0.22% 116 40984 0.22% 117 41414 0.22% 118 40978 0.22% 119 41188 0.22% 120 43123 0.23% 121 43796 0.24% 122 46376 0.25% 123 49446 0.27% 124 51872 0.28% 125 55365 0.30% 126 56274 0.30% 127 57062 0.31% 128 58527 0.31% 129 58531 0.31% 130 59854 0.32% 131 60053 0.32% 132 62628 0.34% 133 66770 0.36% 134 71309 0.38% 135 73325 0.39% 136 76187 0.41% 137 78691 0.42% 138 82344 0.44% 139 87249 0.47% 140 87378 0.47% 141 93969 0.51% 142 99719 0.54% 143 103407 0.56% 144 117068 0.63% 145 132303 0.71% 146 156878 0.84% 147 210137 1.13% 148 357567 1.92% 149 2717561 14.62% 150 12423853 66.82% 18593607 reads passed initial QC criterion=sequence-density sequence-density=0.71 sequence-density-rank=1 fanout-score=2.47 fanout-score-rank=27 prefix-density=0.58 prefix-fanout=2.5 sequence=GTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAACCCAAAAACTTTGATTTCTCATAAGGTGCTGGCGGAGTCCTAAAAGCAACATCCGCCAATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATCCAAGAATTTCACCTCTGACTATGAAATACGAATGCCCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCACCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCGCCGGTAGAAGGGACGAGGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=20.01 fanout-score-rank=1 prefix-density=0.04 prefix-fanout=4.9 sequence=TGGCAGCAAGGCCACTCTGCCACTTACAATACCCCGTCGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGCCCGCGCGGCTCTTTCACCGCGAGGGCTTGGCCAACGGCACGTGCCTCCGGGGCCAAGAGGCCCCTACTGCAGGTCGGCAATCGGACGGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAACGCACGGTAGCTTCGCGCCACTGGCTTTTCAACCAAGCGCGATGACCAATTGTGCGAATCAACGGTTCCTCTCGTACTAGGTTGGATTACTATTGCGACACTGTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAA criterion=sequence-density sequence-density=1.14 sequence-density-rank=1 fanout-score=2.61 fanout-score-rank=28 prefix-density=1.15 prefix-fanout=2.6 sequence=TTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGACAGTGTCGCAATAGTAATCCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTACCGTGCGTTGGATTATGACTGAACGCCTCTAAGTCAGAATCCGGGCTAGATGCGACGCGTGCGCCCGCCGTCCGATTGCCGACCTGCAGTAGGGGCCTCTTGGCCCCGGAGGCACGTGCCGTTGGCCAAGCCCTCGCGGTGAAAGAGCCGCGCGGGCCGCCTTGAAGTACAATTCCCACCGAGCGGCGGGTAGAATCCTTTGCAGACGACTTAAATACGCGACGGGGTATTGTAAGTGGCAGAGTGGCC criterion=fanout-score sequence-density=0.10 sequence-density-rank=20 fanout-score=34.70 fanout-score-rank=1 prefix-density=3.31 prefix-fanout=1.0 sequence=ACGAGACCTCAACCTGCTAACTA SRR5487668 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 06:20:11 Started mapping on | Feb 12 06:20:11 Finished on | Feb 12 06:29:35 Mapping speed, Million of reads per hour | 118.68 Number of input reads | 18593607 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 7549540 Uniquely mapped reads % | 40.60% Average mapped length | 291.49 Number of splices: Total | 5302598 Number of splices: Annotated (sjdb) | 5212015 Number of splices: GT/AG | 5197575 Number of splices: GC/AG | 89858 Number of splices: AT/AC | 3644 Number of splices: Non-canonical | 11521 Mismatch rate per base, % | 0.46% Deletion rate per base | 0.02% Deletion average length | 2.05 Insertion rate per base | 0.04% Insertion average length | 2.31 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 760715 % of reads mapped to multiple loci | 4.09% Number of reads mapped to too many loci | 6513459 % of reads mapped to too many loci | 35.03% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 15.09% % of reads unmapped: other | 5.19% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 10294090 10294090 10294090 N_multimapping 760715 760715 760715 N_noFeature 1268929 7286547 1324780 N_ambiguous 244933 1334 36713 UnstrandedReadsAssigned:6035678 PositiveStrandReadsAssigned:261659 NegativeStrandReadsAssigned:6188047 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=149 echo kmer=145 SRR5487668 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR5487668-trimmed-pair1.fastq SRR5487668-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,593,607 reads, 10,717,034 reads pseudoaligned [quant] estimated average fragment length: 219.627 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,049 rounds 52401 SRR5487668.ke.tsv 34699 SRR5487668.se.tsv 87100 total ==> SRR5487668.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1799.37 143 4.98335 Potri.005G024800.1.v4.1 1035 816.373 98 7.52738 Potri.004G059700.1.v4.1 961 742.379 5 0.422329 Potri.007G009000.2.v4.1 1416 1197.37 1 0.0523694 Potri.003G141000.2.v4.1 2943 2724.37 210 4.83347 Potri.016G087400.1.v4.1 270 81.7562 270 207.085 Potri.015G069301.1.v4.1 564 346.848 0 0 Potri.010G195200.1.v4.1 1773 1554.37 9 0.363073 Potri.012G127500.1.v4.1 977 758.373 452 37.3734 ==> SRR5487668.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 118 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 121 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 44 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 2 SRR5487668 completed mapping pipeline successfully