Starting /dee2/code/volunteer_pipeline.sh SRR6031373 current disk space = 2796418781184 free memory = 1575039976 SRR6031373_1.fastq is conventional basespace SRR6031373_1.fastq read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6031373_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20486938 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0 30.0 30.0 30.0 30.0 30.0 2 30.0 30.0 30.0 30.0 30.0 30.0 3 30.0 30.0 30.0 30.0 30.0 30.0 4 30.0 30.0 30.0 30.0 30.0 30.0 5 30.0 30.0 30.0 30.0 30.0 30.0 6 30.0 30.0 30.0 30.0 30.0 30.0 7 30.0 30.0 30.0 30.0 30.0 30.0 8 30.0 30.0 30.0 30.0 30.0 30.0 9 30.0 30.0 30.0 30.0 30.0 30.0 10-14 30.0 30.0 30.0 30.0 30.0 30.0 15-19 30.0 30.0 30.0 30.0 30.0 30.0 20-24 30.0 30.0 30.0 30.0 30.0 30.0 25-29 30.0 30.0 30.0 30.0 30.0 30.0 30-34 30.0 30.0 30.0 30.0 30.0 30.0 35-39 30.0 30.0 30.0 30.0 30.0 30.0 40-44 30.0 30.0 30.0 30.0 30.0 30.0 45-49 30.0 30.0 30.0 30.0 30.0 30.0 50-54 30.0 30.0 30.0 30.0 30.0 30.0 55-59 30.0 30.0 30.0 30.0 30.0 30.0 60-64 30.0 30.0 30.0 30.0 30.0 30.0 65-69 30.0 30.0 30.0 30.0 30.0 30.0 70-74 30.0 30.0 30.0 30.0 30.0 30.0 75-79 30.0 30.0 30.0 30.0 30.0 30.0 80-84 30.0 30.0 30.0 30.0 30.0 30.0 85-89 30.0 30.0 30.0 30.0 30.0 30.0 90-94 30.0 30.0 30.0 30.0 30.0 30.0 95-99 30.0 30.0 30.0 30.0 30.0 30.0 100-104 30.0 30.0 30.0 30.0 30.0 30.0 105-109 30.0 30.0 30.0 30.0 30.0 30.0 110-114 30.0 30.0 30.0 30.0 30.0 30.0 115-119 30.0 30.0 30.0 30.0 30.0 30.0 120-124 30.0 30.0 30.0 30.0 30.0 30.0 125-129 30.0 30.0 30.0 30.0 30.0 30.0 130-134 30.0 30.0 30.0 30.0 30.0 30.0 135-139 30.0 30.0 30.0 30.0 30.0 30.0 140-144 30.0 30.0 30.0 30.0 30.0 30.0 145-149 30.0 30.0 30.0 30.0 30.0 30.0 150 30.0 30.0 30.0 30.0 30.0 30.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 2.0486938E7 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.28634760522171 14.396268811452895 8.81828107677326 32.49910250655214 2 22.60792217948822 17.820276509842515 34.470578277729935 25.10122303293933 3 19.001961152027697 25.246247145376238 27.389930110590466 28.3618615920056 4 22.854152937386967 31.905776233075006 23.014337968499238 22.22573286103879 5 21.872677780574882 35.87392397933665 23.628776117980514 18.624622122107947 6 17.508775591550087 35.96920633039451 25.406310108421277 21.115707969634116 7 13.978247993916904 23.79115414904853 43.5655098873243 18.66508796971026 8 16.97825707287248 23.53247713250267 31.673454568955105 27.81581122566974 9 17.52372170013889 23.497279095587636 32.98419217161686 25.994807032656613 10-14 20.025478673289292 29.681904636017347 26.687358550116176 23.60525814057718 15-19 19.99813500665722 28.644942735313027 27.662576235654246 23.694346022375505 20-24 19.923274614552874 28.694797056138473 27.725741833069435 23.656186496239222 25-29 19.90674407587306 28.92644787304049 27.6484401687741 23.51836788231235 30-34 19.978091406339004 28.821266506493064 27.557043419568117 23.643598667599814 35-39 20.010935748426633 28.70743007080902 27.59009179409827 23.69154238666608 40-44 20.088311879500978 28.846671962398673 27.547555422874808 23.517460735225537 45-49 20.14336530499054 28.677261723631386 27.553202047204987 23.62617092417309 50-54 20.145763977723316 28.608982414893337 27.583597797916713 23.661655809466634 55-59 20.13849960225322 28.64633286994136 27.544216101060776 23.670951426744644 60-64 20.094488584089735 28.563909611482607 27.56540405933743 23.77619774509023 65-69 20.104224934969487 28.441837046482988 27.677614966234255 23.77632305231327 70-74 20.196247806711014 28.569706627469287 27.603840410038604 23.63020515578109 75-79 20.24909132523667 28.490881133972607 27.524543114624155 23.735484426166572 80-84 20.209017636263315 28.479020005686184 27.631392190815284 23.680570167235217 85-89 20.3193917997897 28.472145520233425 27.557643802114303 23.650818877862566 90-94 20.348546961971575 28.40755997797231 27.508624275623816 23.735268784432307 95-99 20.34161181138929 28.33340834047528 27.619054638619005 23.705925209516423 100-104 20.394828755614785 28.48434339434258 27.599072958557485 23.52175489148515 105-109 20.378388872613467 28.35205937555154 27.611105772746612 23.658445979088377 110-114 20.479720483418454 28.318013000435684 27.59004877718671 23.612217738959153 115-119 20.610141345551042 28.4159250105731 27.425606261109657 23.5483273827662 120-124 20.523042354794228 28.33167744584793 27.465981772390425 23.679298426967417 125-129 20.61240662171796 28.21282041124234 27.472895265183904 23.701877701855796 130-134 20.675563535664793 28.337102954811556 27.39930637559027 23.58802713393338 135-139 20.81002734522846 28.247941200388265 27.283956245682006 23.65807520870127 140-144 20.748544267571855 28.190363049861332 27.392695775230052 23.668396907336763 145-149 20.784673629607315 28.366014482008 27.229245287900028 23.62006660048466 150 20.678555524801943 27.951874547686916 27.443851838716988 23.925718088794152 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3185.0 1 2557.5 2 1660.0 3 1307.0 4 1119.5 5 1001.0 6 930.0 7 843.0 8 827.5 9 788.5 10 723.5 11 718.5 12 799.5 13 908.5 14 1020.0 15 1224.5 16 1425.0 17 1657.0 18 2068.0 19 2657.0 20 3470.0 21 4750.5 22 6556.5 23 9267.0 24 13209.5 25 18074.5 26 25199.5 27 35694.0 28 49666.0 29 69430.0 30 95212.0 31 126199.5 32 163520.0 33 210146.0 34 272344.5 35 351759.5 36 444376.5 37 550588.0 38 674496.0 39 816061.5 40 970067.0 41 1128233.5 42 1258831.0 43 1342699.0 44 1390633.0 45 1401233.5 46 1369658.5 47 1298365.5 48 1200058.0 49 1065795.5 50 898093.0 51 732801.5 52 599372.0 53 487024.0 54 371788.0 55 268550.0 56 194886.0 57 143309.0 58 104991.5 59 80186.5 60 61076.5 61 43960.0 62 32134.5 63 24533.5 64 16933.0 65 11153.0 66 7771.0 67 5531.0 68 4328.0 69 3059.0 70 1819.0 71 976.5 72 606.5 73 322.0 74 152.0 75 67.0 76 29.5 77 19.5 78 14.5 79 9.5 80 5.5 81 3.5 82 3.5 83 2.0 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0918731730432337 2 0.0 3 0.0 4 2.4405794560416984E-5 5 0.026621840706502847 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 7.809854259333435E-6 20-24 7.770804988036768E-4 25-29 1.7572172083500228E-5 30-34 0.0 35-39 0.0 40-44 0.0 45-49 3.9049271296667176E-6 50-54 5.271651625050068E-5 55-59 1.6791186657566883E-4 60-64 0.0021574722391408613 65-69 8.327257104014276E-4 70-74 6.130735593576747E-4 75-79 0.0011470723443395984 80-84 4.519953152589225E-4 85-89 0.0 90-94 0.0 95-99 0.0 100-104 2.928695347250038E-6 105-109 6.931245655158423E-5 110-114 1.2007650923725156E-4 115-119 1.9134142935366914E-4 120-124 1.893889657888358E-4 125-129 3.9049271296667176E-6 130-134 1.9524635648333588E-6 135-139 0.0 140-144 0.0 145-149 0.0 150 0.13095661245228546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 2.0486938E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.50883358885656 #Duplication Level Percentage of deduplicated Percentage of total 1 64.44695961767349 26.751181220777674 2 17.144077870946774 14.232613507590559 3 6.631719980165145 8.258248831937104 4 3.3686843519169325 5.593206327084204 5 2.0304544503910593 4.214089794551786 6 1.329997725299243 3.312399255180243 7 0.9585123536456938 2.7850710846239632 8 0.6761686547193607 2.2453577733397565 9 0.4923159891930021 1.839191622169107 >10 2.6948344608934485 19.745334715374998 >50 0.15459880245595906 4.403626858819954 >100 0.06737718289174438 4.917376399004868 >500 0.0031205062970704853 0.8973320037112462 >1k 0.0011662910032454077 0.778707746724261 >5k 1.1762507946720289E-5 0.026262859110353753 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.003825461862578E-4 9.762317824166794E-6 0.0 0.0 0.0 2 6.15026022922508E-4 2.4405794560416984E-5 0.0 9.762317824166794E-6 0.0 3 6.443129763950083E-4 2.928695347250038E-5 0.0 9.762317824166794E-6 0.0 4 7.272926779004262E-4 3.904927129666718E-5 0.0 9.762317824166794E-6 0.0 5 7.468173135487598E-4 3.904927129666718E-5 0.0 1.464347673625019E-5 0.0 6 7.7610426702126E-4 3.904927129666718E-5 0.0 1.952463564833359E-5 0.0 7 7.956289026695936E-4 6.345506585708416E-5 0.0 1.952463564833359E-5 0.0 8 8.151535383179272E-4 7.321738368125096E-5 0.0 1.952463564833359E-5 0.0 9 8.24915856142094E-4 7.321738368125096E-5 0.0 2.4405794560416984E-5 0.0 10-11 9.347419316639705E-4 9.518259878562624E-5 0.0 2.684637401645868E-5 0.0 12-13 0.001003078156433138 1.0738549606583473E-4 0.0 3.172753292854208E-5 0.0 14-15 0.0010543303250100137 1.1226665497791812E-4 0.0 3.904927129666718E-5 0.0 16-17 0.0011104636524989727 1.2446955225812662E-4 0.0 5.857390694500076E-5 0.0 18-19 0.0011543940827077235 1.391130289943768E-4 0.0 6.833622476916756E-5 0.0 20-21 0.0012178491485648076 1.415536084504185E-4 0.0 6.833622476916756E-5 0.0 22-23 0.0012935071117021 1.439941879064602E-4 0.0 7.565796313729266E-5 0.0 24-25 0.0013398781213668923 1.488753468185436E-4 0.0 9.274201932958454E-5 0.0 26-27 0.0013911302899437682 1.513159262745853E-4 0.0 1.0006375769770964E-4 0.0 28-29 0.0014423824585206436 1.513159262745853E-4 0.0 1.0982607552187642E-4 0.0 30-31 0.0015351244778502283 1.561970851866687E-4 0.0 1.3179129062625171E-4 0.0 32-33 0.0016473911328281463 1.6839998246687718E-4 0.0 1.488753468185436E-4 0.0 34-35 0.0017352519932456476 1.7572172083500228E-4 0.0 1.53756505730627E-4 0.0 36-37 0.0018109099563829402 1.8548403865916907E-4 0.0 1.561970851866687E-4 0.0 38-39 0.0018890084989762745 1.8548403865916907E-4 0.0 1.561970851866687E-4 0.0 40-41 0.0019890722566739843 1.9768693593937757E-4 0.0 1.6351882355479378E-4 0.0 42-43 0.0020988983321958607 2.2209273049979457E-4 0.0 1.6595940301083548E-4 0.0 44-45 0.0022355707817341957 2.2697388941187794E-4 0.0 1.6595940301083548E-4 0.0 46-47 0.0024137330820252394 2.4893910451625323E-4 0.0 1.7084056192291888E-4 0.0 48-49 0.002628504074156909 2.562608428843783E-4 0.0 1.7816230029104398E-4 0.0 50-51 0.0028847649170412873 2.782260579887536E-4 0.0 1.8304345920312738E-4 0.0 52-53 0.0031410257599256658 3.221564881975042E-4 0.0 2.0012751539541927E-4 0.0 54-55 0.003570567744189005 3.3191880602167096E-4 0.0 2.1477099213166947E-4 0.0 56-57 0.004019634364100678 3.4168112384583776E-4 0.0 2.3185504832396134E-4 0.0 58-59 0.004580967638990268 3.5632460058208795E-4 0.0 2.3917678669208644E-4 0.0 60-61 0.005278973363418193 4.0513618970292194E-4 0.0 2.3917678669208644E-4 0.0 62-63 0.006130735593576747 4.2954198426333894E-4 0.0 2.5137968397229496E-4 0.0 64-65 0.007324178947581137 4.466260404556308E-4 0.0 2.855477963568787E-4 0.0 66-67 0.008583517946898652 4.6126951719188095E-4 0.0 3.3191880602167096E-4 0.0 68-69 0.010250433715375134 4.8079415284021454E-4 0.0 3.538840211260463E-4 0.0 70-71 0.012193134962384326 4.8811589120833967E-4 0.0 3.5632460058208795E-4 0.0 72-73 0.014853366569469777 4.978782090325066E-4 0.0 3.587651800381296E-4 0.0 74-75 0.018543522707004823 5.027593679445899E-4 0.0 3.7584923623042155E-4 0.0 76-77 0.022885313559303004 5.076405268566733E-4 0.0 3.9537387187875514E-4 0.0 78-79 0.02809595069795203 5.125216857687566E-4 0.0 4.1001734861500534E-4 0.0 80-81 0.03466843117307233 5.247245830489651E-4 0.0 4.3442314317542233E-4 0.0 82-83 0.043076227399135976 5.515709570654238E-4 0.0 4.539477788237559E-4 0.0 84-85 0.053919721922329245 5.808579105379242E-4 0.0 4.6859125556000607E-4 0.0 86-87 0.06698170317106442 5.90620228362091E-4 2.4405794560416986E-6 4.7347241447208947E-4 0.0 88-89 0.08239884359487981 5.90620228362091E-4 4.881158912083397E-6 4.905564706643813E-4 0.0 90-91 0.1007520011043134 5.955013872741744E-4 4.881158912083397E-6 4.978782090325065E-4 0.0 92-93 0.12255613796458993 6.052637050983412E-4 4.881158912083397E-6 5.125216857687566E-4 0.0 94-95 0.1497710394789109 6.15026022922508E-4 4.881158912083397E-6 5.125216857687566E-4 0.0 96-97 0.18161327964188695 6.15026022922508E-4 4.881158912083397E-6 5.198434241368817E-4 0.0 98-99 0.21786808746138636 6.247883407466748E-4 4.881158912083397E-6 5.320463214170902E-4 0.0 100-101 0.25862820495673877 6.589564531312585E-4 4.881158912083397E-6 5.418086392412571E-4 0.0 102-103 0.3053775044372175 7.004463038839674E-4 4.881158912083397E-6 5.466897981533405E-4 0.0 104-105 0.3603344726283645 7.272926779004262E-4 4.881158912083397E-6 5.491303776093821E-4 0.0 106-107 0.42250823427102674 7.541390519168848E-4 4.881158912083397E-6 5.515709570654238E-4 0.0 108-109 0.4902196706994476 8.102723794058439E-4 4.881158912083397E-6 5.515709570654238E-4 0.0 110-111 0.5642888166108571 8.151535383179272E-4 4.881158912083397E-6 5.66214433801674E-4 0.0 112-113 0.6462507964831055 8.17594117773969E-4 4.881158912083397E-6 5.66214433801674E-4 0.0 114-115 0.736476578393511 8.224752766860524E-4 4.881158912083397E-6 5.686550132577157E-4 0.0 116-117 0.836384139006034 8.346781739662608E-4 4.881158912083397E-6 5.784173310818825E-4 0.0 118-119 0.9475012810601564 8.444404917904276E-4 4.881158912083397E-6 5.808579105379242E-4 0.0 120-121 1.0664453614298046 8.590839685266779E-4 4.881158912083397E-6 5.808579105379242E-4 0.0 122-123 1.1940364148122087 8.712868658068863E-4 4.881158912083397E-6 5.808579105379242E-4 0.0 124-125 1.3346040291623864 8.786086041750115E-4 4.881158912083397E-6 5.808579105379242E-4 0.0 126-127 1.4900787028300666 8.98133239823345E-4 4.881158912083397E-6 5.808579105379242E-4 0.0 128-129 1.6571339260166649 9.005738192793867E-4 4.881158912083397E-6 5.857390694500076E-4 0.0 130-131 1.8357208871330601 9.103361371035534E-4 4.881158912083397E-6 5.90620228362091E-4 4.881158912083397E-6 132-133 2.024875557294116 9.200984549277203E-4 4.881158912083397E-6 5.930608078181328E-4 4.881158912083397E-6 134-135 2.229088602698949 9.347419316639705E-4 4.881158912083397E-6 5.955013872741744E-4 4.881158912083397E-6 136-137 2.449001895744498 9.542665673123041E-4 4.881158912083397E-6 6.003825461862578E-4 4.881158912083397E-6 138 2.6248822542441435 9.567071467683457E-4 4.881158912083397E-6 6.003825461862578E-4 4.881158912083397E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACT 4365 0.0 14.201214 1 GTCGGTT 4735 0.0 13.852645 1 GTCGCTT 5565 0.0 13.59989 1 GTCCGAT 4430 0.0 12.853892 1 GTCGAAT 10025 0.0 12.7981415 1 GTCGCAT 5570 0.0 12.681837 1 GTCGATT 7170 0.0 11.761913 1 GTCGGAA 4270 0.0 11.647493 1 GTCCGCT 4310 0.0 11.372159 1 GTCGAGT 4875 0.0 10.793431 1 GTCGTAT 2755 0.0 10.726883 1 GTCACGT 4185 0.0 10.678432 1 GTCGGAT 4375 0.0 10.544189 1 CGGACGT 1310 1.8553692E-10 10.4542675 1 GCCAGTT 24325 0.0 10.430403 1 GGGGGAT 7755 0.0 10.409923 1 GCGGGAT 3480 0.0 10.356237 1 GCCAATT 25730 0.0 10.112969 1 GTTGGAT 27345 0.0 9.858366 1 GCCGGTT 4610 0.0 9.850332 1 >>END_MODULE SRR6031373 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6031373_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20486938 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0 30.0 30.0 30.0 30.0 30.0 2 30.0 30.0 30.0 30.0 30.0 30.0 3 30.0 30.0 30.0 30.0 30.0 30.0 4 30.0 30.0 30.0 30.0 30.0 30.0 5 30.0 30.0 30.0 30.0 30.0 30.0 6 30.0 30.0 30.0 30.0 30.0 30.0 7 30.0 30.0 30.0 30.0 30.0 30.0 8 30.0 30.0 30.0 30.0 30.0 30.0 9 30.0 30.0 30.0 30.0 30.0 30.0 10-14 30.0 30.0 30.0 30.0 30.0 30.0 15-19 30.0 30.0 30.0 30.0 30.0 30.0 20-24 30.0 30.0 30.0 30.0 30.0 30.0 25-29 30.0 30.0 30.0 30.0 30.0 30.0 30-34 30.0 30.0 30.0 30.0 30.0 30.0 35-39 30.0 30.0 30.0 30.0 30.0 30.0 40-44 30.0 30.0 30.0 30.0 30.0 30.0 45-49 30.0 30.0 30.0 30.0 30.0 30.0 50-54 30.0 30.0 30.0 30.0 30.0 30.0 55-59 30.0 30.0 30.0 30.0 30.0 30.0 60-64 30.0 30.0 30.0 30.0 30.0 30.0 65-69 30.0 30.0 30.0 30.0 30.0 30.0 70-74 30.0 30.0 30.0 30.0 30.0 30.0 75-79 30.0 30.0 30.0 30.0 30.0 30.0 80-84 30.0 30.0 30.0 30.0 30.0 30.0 85-89 30.0 30.0 30.0 30.0 30.0 30.0 90-94 30.0 30.0 30.0 30.0 30.0 30.0 95-99 30.0 30.0 30.0 30.0 30.0 30.0 100-104 30.0 30.0 30.0 30.0 30.0 30.0 105-109 30.0 30.0 30.0 30.0 30.0 30.0 110-114 30.0 30.0 30.0 30.0 30.0 30.0 115-119 30.0 30.0 30.0 30.0 30.0 30.0 120-124 30.0 30.0 30.0 30.0 30.0 30.0 125-129 30.0 30.0 30.0 30.0 30.0 30.0 130-134 30.0 30.0 30.0 30.0 30.0 30.0 135-139 30.0 30.0 30.0 30.0 30.0 30.0 140-144 30.0 30.0 30.0 30.0 30.0 30.0 145-149 30.0 30.0 30.0 30.0 30.0 30.0 150 30.0 30.0 30.0 30.0 30.0 30.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 2.0486938E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09672828772608 20.673033821661456 11.886498680176876 24.343739210435587 2 26.384815847580818 24.821620264387732 31.978550139286526 16.815013748744924 3 21.01035248405872 27.088384581449176 32.42907036560298 19.472192568889128 4 24.033103562948853 34.87517819634731 22.427211529762154 18.664506710941684 5 23.92424675492329 37.91267473391205 21.534671034477395 16.628407476687265 6 19.405326710560765 38.059379847666264 23.58122839450694 18.954065047266035 7 19.390381565382985 19.25434158629507 40.888451762403186 20.466825085918767 8 20.095453244068214 24.08745781791022 28.808059236297805 27.00902970172376 9 21.6950427859367 24.57598810019572 29.320598139541293 24.40837097432629 10-14 23.18582674509741 28.594409793529096 26.65962129282659 21.560142168546907 15-19 22.97785703153671 28.053894655046047 28.009858306642098 20.958390006775147 20-24 22.790390585305097 28.246676709668066 28.003544871701237 20.9593878333256 25-29 22.917426944310087 28.249564524700038 27.986067450150625 20.84694108083925 30-34 22.826364827274286 27.963801393407238 28.31435508330667 20.895478696011804 35-39 22.89802216491548 28.07291074930428 28.12578665033762 20.903280435442618 40-44 23.109529209541186 27.895410841623747 28.16306087828922 20.831999070545848 45-49 23.076241848348598 27.703636528013746 28.30167466928867 20.918446954348994 50-54 23.038798439008026 27.80591938571498 28.235592109481455 20.91969006579554 55-59 23.212100844097485 27.63338561408666 28.194551581634457 20.9599619601814 60-64 23.10624115664667 27.638510081426464 28.31879312272591 20.936455639200954 65-69 23.217151163928204 27.653230003482587 28.257514957524155 20.872103875065054 70-74 23.316367556194102 27.706833038214373 28.078120154818432 20.898679250773093 75-79 23.26366378331948 27.590129824010962 28.227593431041885 20.918612961627677 80-84 23.312956857182858 27.706496736046372 28.068215011362195 20.91233139540858 85-89 23.4572555881795 27.81054280876955 27.901858646145232 20.830342956905717 90-94 23.39534733722984 27.82475183143365 27.997043484080365 20.782857347256144 95-99 23.348137804717606 27.822146035941635 27.98737523297573 20.84234092636503 100-104 23.573179290114314 27.760840070799176 27.9329568999771 20.73302373910941 105-109 23.511741419400863 27.71546202488644 28.046141859083797 20.726654696628906 110-114 23.523765946177342 27.776116426359533 28.00118149869233 20.698936128770796 115-119 23.66254010519583 27.775631414903717 27.93280362550109 20.62902485439936 120-124 23.704492157418763 27.766025338066925 27.91708032762535 20.61240217688896 125-129 23.73219978276172 27.896817316257287 27.820971919342206 20.550010981638785 130-134 23.863005711263625 27.907160581675118 27.752789895243335 20.47704381181792 135-139 23.832078717700714 27.831854220243756 27.855253221429503 20.480813840626023 140-144 23.913312142552915 27.884230507102448 27.76419465839203 20.43826269195261 145-149 24.19560341012709 27.822344205230255 27.670282940740858 20.3117694439018 150 24.010821555418755 27.558592698655353 28.16079557076683 20.269790175159063 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7429.0 1 6621.0 2 4028.5 3 2002.5 4 1605.0 5 1376.0 6 1268.5 7 1230.0 8 1242.0 9 1293.5 10 1283.0 11 1326.5 12 1463.5 13 1562.0 14 1678.5 15 1910.5 16 2192.5 17 2532.5 18 3039.5 19 3577.5 20 4274.0 21 5328.0 22 6790.0 23 8834.0 24 11762.0 25 15875.0 26 21329.5 27 28584.0 28 38317.5 29 51623.5 30 69253.0 31 91587.0 32 123645.0 33 167749.5 34 225737.0 35 304658.0 36 411342.5 37 540707.0 38 681878.0 39 842431.0 40 1016857.0 41 1184891.5 42 1318613.0 43 1395680.5 44 1435217.5 45 1451250.0 46 1410984.5 47 1310451.5 48 1186487.5 49 1045222.0 50 890281.5 51 738678.0 52 599792.0 53 472855.5 54 358927.5 55 262700.5 56 191149.5 57 143004.5 58 106175.5 59 78923.5 60 57392.0 61 39289.5 62 28476.0 63 21101.5 64 14632.0 65 9931.0 66 6563.5 67 4407.0 68 3210.5 69 2258.5 70 1562.5 71 1137.5 72 671.0 73 393.5 74 235.5 75 165.5 76 132.5 77 97.0 78 72.0 79 55.0 80 49.5 81 47.0 82 38.0 83 29.5 84 28.5 85 26.0 86 24.5 87 23.5 88 15.5 89 8.5 90 11.5 91 12.0 92 7.0 93 5.0 94 4.5 95 4.5 96 4.5 97 3.5 98 3.0 99 4.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0911068310940366 2 0.15609946200842703 3 0.18845666443662787 4 0.19030662366430745 5 0.21650380354545903 6 0.1240644160684237 7 0.12711025922956373 8 0.12808160985306832 9 0.12410346533972036 10-14 0.13727283208452137 15-19 0.15850001596138963 20-24 0.15472492765878434 25-29 0.16539123611346898 30-34 0.15472102273165467 35-39 0.11126601740094103 40-44 0.10959958974835575 45-49 0.14977445629014938 50-54 0.2119633495254391 55-59 0.19599219756510222 60-64 0.19427891078696094 65-69 0.20685375237627016 70-74 0.22081191440126388 75-79 0.16537464017316789 80-84 0.14762674636883266 85-89 0.12839009909631202 90-94 0.16568996303888847 95-99 0.16780643354316785 100-104 0.17885835355190707 105-109 0.21045116649447568 110-114 0.21024518158838573 115-119 0.2303536038426045 120-124 0.23182576137048883 125-129 0.1956407541234322 130-134 0.1664045647036175 135-139 0.1405764004362194 140-144 0.1141439486955054 145-149 0.15439105638919784 150 0.2528342693280958 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 2.0486938E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.085907569520316 #Duplication Level Percentage of deduplicated Percentage of total 1 65.55012344030501 28.242865597196285 2 16.832634903746317 14.504987032285904 3 6.558590417605406 8.477484615578646 4 3.283741030529745 5.659318500945839 5 1.9828713662995723 4.271690620531591 6 1.2578523745504995 3.2517426687590683 7 0.8676170523540406 2.61674476864261 8 0.6063337431110849 2.089955168957238 9 0.45783388097749994 1.7753569450192193 >10 2.405852444126671 18.175383604276874 >50 0.12883364118661483 3.7921147614823925 >100 0.06272820379953449 4.913392375258897 >500 0.003613310502367301 1.0609582628293157 >1k 0.001272111023405838 0.8982192227051022 >5k 1.0207988202062656E-4 0.2697858555312174 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.808579105379242E-4 0.0 0.0 0.0 0.0 2 6.101448640104246E-4 0.0 9.762317824166794E-6 1.464347673625019E-5 0.0 3 6.101448640104246E-4 0.0 9.762317824166794E-6 2.4405794560416984E-5 0.0 4 6.443129763950083E-4 0.0 4.881158912083397E-5 3.904927129666718E-5 0.0 5 6.589564531312586E-4 0.0 6.833622476916756E-5 5.857390694500076E-5 0.0 6 6.88243406603759E-4 0.0 7.321738368125096E-5 6.345506585708416E-5 0.0 7 6.88243406603759E-4 4.881158912083397E-6 7.321738368125096E-5 6.345506585708416E-5 0.0 8 7.077680422520926E-4 4.881158912083397E-6 7.321738368125096E-5 6.345506585708416E-5 0.0 9 7.175303600762594E-4 4.881158912083397E-6 7.809854259333435E-5 7.321738368125096E-5 0.0 10-11 7.687825286531349E-4 4.881158912083397E-6 7.809854259333435E-5 7.565796313729266E-5 0.0 12-13 7.956289026695936E-4 4.881158912083397E-6 7.809854259333435E-5 8.786086041750114E-5 0.0 14-15 8.24915856142094E-4 9.762317824166794E-6 7.809854259333435E-5 1.1226665497791812E-4 0.0 16-17 8.664057068948029E-4 1.464347673625019E-5 7.809854259333435E-5 1.1226665497791812E-4 0.0 18-19 8.956926603673033E-4 1.952463564833359E-5 7.809854259333435E-5 1.1226665497791812E-4 0.0 20-21 9.29860772751887E-4 2.1965215104375288E-5 8.297970150541775E-5 1.1226665497791812E-4 0.0 22-23 9.95756418065013E-4 2.928695347250038E-5 8.786086041750114E-5 1.1470723443395982E-4 0.0 24-25 0.0010348056893616801 2.928695347250038E-5 8.786086041750114E-5 1.415536084504185E-4 0.0 26-27 0.0010836172784825142 2.928695347250038E-5 9.274201932958454E-5 1.561970851866687E-4 0.0 28-29 0.0011299882881473063 2.928695347250038E-5 9.274201932958454E-5 1.6107824409875208E-4 0.0 30-31 0.001205646251284599 3.172753292854208E-5 9.274201932958454E-5 1.6839998246687718E-4 0.0 32-33 0.0013081505884383502 3.416811238458378E-5 1.0250433715375134E-4 2.1233041267562777E-4 0.0 34-35 0.0013960114488558513 3.416811238458378E-5 1.0738549606583473E-4 2.1965215104375287E-4 0.0 36-37 0.0014741099914491857 3.660869184062548E-5 1.1714781389000152E-4 2.1965215104375287E-4 0.0 38-39 0.0015424462162183534 4.881158912083397E-5 1.1714781389000152E-4 2.3185504832396134E-4 2.4405794560416986E-6 40-41 0.0016327476560918962 4.881158912083397E-5 1.1714781389000152E-4 2.4161736614812813E-4 4.881158912083397E-6 42-43 0.0017498954699818976 4.881158912083397E-5 1.1958839334604322E-4 2.5137968397229496E-4 4.881158912083397E-6 44-45 0.001869483863327941 4.881158912083397E-5 1.3179129062625171E-4 2.879883758129204E-4 4.881158912083397E-6 46-47 0.0020330026868827346 4.881158912083397E-5 1.391130289943768E-4 3.0019127309312895E-4 4.881158912083397E-6 48-49 0.0022380113611902373 5.369274803291737E-5 1.415536084504185E-4 3.148347498293791E-4 4.881158912083397E-6 50-51 0.002491831624618574 5.857390694500076E-5 1.464347673625019E-4 3.2947822656562923E-4 4.881158912083397E-6 52-53 0.0027456518880469107 5.857390694500076E-5 1.464347673625019E-4 3.734086567743799E-4 4.881158912083397E-6 54-55 0.003172753292854208 5.857390694500076E-5 1.513159262745853E-4 4.0025503079083854E-4 4.881158912083397E-6 56-57 0.0036315822305900474 6.345506585708416E-5 1.513159262745853E-4 4.0513618970292194E-4 4.881158912083397E-6 58-59 0.004185593767111513 6.345506585708416E-5 1.6107824409875208E-4 4.271014048072972E-4 4.881158912083397E-6 60-61 0.004859193696979021 7.321738368125096E-5 1.6107824409875208E-4 4.539477788237559E-4 4.881158912083397E-6 62-63 0.005713396506593616 7.321738368125096E-5 1.6107824409875208E-4 4.6859125556000607E-4 4.881158912083397E-6 64-65 0.006901958701685923 7.321738368125096E-5 1.6107824409875208E-4 4.905564706643813E-4 4.881158912083397E-6 66-67 0.008129570168074897 7.321738368125096E-5 1.6107824409875208E-4 5.100811063127149E-4 4.881158912083397E-6 68-69 0.009720827973414085 7.565796313729266E-5 1.8792461811521077E-4 5.393680597852153E-4 4.881158912083397E-6 70-71 0.011673291538247442 8.542028096145944E-5 1.9524635648333587E-4 6.052637050983412E-4 4.881158912083397E-6 72-73 0.014292033294580186 8.786086041750114E-5 1.9524635648333587E-4 6.125854434664664E-4 4.881158912083397E-6 74-75 0.0178748039360494 9.274201932958454E-5 1.9524635648333587E-4 6.247883407466748E-4 4.881158912083397E-6 76-77 0.022148258563578414 9.274201932958454E-5 2.2209273049979457E-4 6.418723969389667E-4 4.881158912083397E-6 78-79 0.027283237739090145 9.274201932958454E-5 2.2453330995583624E-4 6.63837612043342E-4 4.881158912083397E-6 80-81 0.03368243707283148 1.0738549606583473E-4 2.2453330995583624E-4 6.760405093235505E-4 9.762317824166794E-6 82-83 0.04194623911098867 1.0738549606583473E-4 2.3429562778000304E-4 6.980057244279257E-4 9.762317824166794E-6 84-85 0.05262133365171506 1.0738549606583473E-4 2.3917678669208644E-4 7.028868833400091E-4 9.762317824166794E-6 86-87 0.06563694389078543 1.0738549606583473E-4 2.6358258125250343E-4 7.102086217081342E-4 9.762317824166794E-6 88-89 0.08103455967895251 1.0738549606583473E-4 2.8066663744479535E-4 7.175303600762594E-4 9.762317824166794E-6 90-91 0.09928765343068838 1.0982607552187642E-4 2.879883758129204E-4 7.175303600762594E-4 9.762317824166794E-6 92-93 0.12082088597134427 1.1714781389000152E-4 2.953101141810455E-4 7.272926779004262E-4 9.762317824166794E-6 94-95 0.1477990512784292 1.2691013171416832E-4 2.977506936370872E-4 7.419361546366764E-4 9.762317824166794E-6 96-97 0.17932645669157588 1.3179129062625171E-4 2.977506936370872E-4 7.516984724608431E-4 9.762317824166794E-6 98-99 0.21513707905007573 1.3179129062625171E-4 2.977506936370872E-4 7.541390519168848E-4 9.762317824166794E-6 100-101 0.25558724295451085 1.3179129062625171E-4 3.026318525491706E-4 7.639013697410516E-4 9.762317824166794E-6 102-103 0.3019875395727756 1.3423187008229341E-4 3.026318525491706E-4 7.858665848454269E-4 9.762317824166794E-6 104-105 0.3564124614425055 1.3667244953833511E-4 3.026318525491706E-4 7.93188323213552E-4 9.762317824166794E-6 106-107 0.4179223854731244 1.3667244953833511E-4 3.07513011461254E-4 7.956289026695936E-4 1.2202897280208492E-5 108-109 0.4849211727003811 1.391130289943768E-4 3.07513011461254E-4 8.102723794058439E-4 1.464347673625019E-5 110-111 0.558253263616066 1.439941879064602E-4 3.148347498293791E-4 8.24915856142094E-4 1.464347673625019E-5 112-113 0.6394513421185732 1.464347673625019E-4 3.2215648819750416E-4 8.24915856142094E-4 1.464347673625019E-5 114-115 0.7290718603238806 1.513159262745853E-4 3.2215648819750416E-4 8.322375945102192E-4 1.464347673625019E-5 116-117 0.8286401803920137 1.53756505730627E-4 3.2215648819750416E-4 8.395593328783443E-4 1.464347673625019E-5 118-119 0.9388152587761041 1.561970851866687E-4 3.3679996493375436E-4 8.517622301585527E-4 1.464347673625019E-5 120-121 1.056858765326473 1.561970851866687E-4 3.4900286221396283E-4 8.542028096145944E-4 1.464347673625019E-5 122-123 1.1834418593935316 1.6107824409875208E-4 3.5144344167000455E-4 8.615245479827195E-4 1.464347673625019E-5 124-125 1.3227159666320072 1.6107824409875208E-4 3.5144344167000455E-4 8.639651274387612E-4 1.464347673625019E-5 126-127 1.4765725361203317 1.6595940301083548E-4 3.5632460058208795E-4 8.73727445262928E-4 1.464347673625019E-5 128-129 1.6420145362864864 1.6595940301083548E-4 3.587651800381296E-4 9.005738192793867E-4 1.464347673625019E-5 130-131 1.8186051034078397 1.7084056192291888E-4 3.6608691840625475E-4 9.323013522079288E-4 1.464347673625019E-5 132-133 2.0057048056669085 1.8060287974708568E-4 3.6608691840625475E-4 9.396230905760539E-4 1.464347673625019E-5 134-135 2.2081948019757762 1.8060287974708568E-4 3.6608691840625475E-4 9.469448289441789E-4 1.464347673625019E-5 136-137 2.4257944256969974 1.8548403865916907E-4 3.6608691840625475E-4 9.615883056804291E-4 1.464347673625019E-5 138 2.5999883437925178 1.8548403865916907E-4 3.9049271296667175E-4 9.908752591529296E-4 1.464347673625019E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCGAC 3525 0.0 13.680863 9 AGCGCAC 3175 0.0 13.38166 5 CAACGTC 8685 0.0 12.016997 8 TAAGCGC 3915 0.0 10.851986 3 CATATAG 9025 0.0 10.053385 3 AACGTCT 10135 0.0 9.942649 9 CTAAGCG 4470 0.0 9.826781 2 ACAACGT 10710 0.0 9.744472 7 GTAGTCT 8075 0.0 9.355483 6 AGTAGTC 7940 0.0 9.250845 5 AATCCAT 29210 0.0 9.244884 5 TTAATCC 11230 0.0 9.10537 3 GCGCACA 4895 0.0 8.965956 6 GTTTAAT 22355 0.0 8.954844 1 GGGAAAT 35085 0.0 8.928031 1 TCAATCC 26180 0.0 8.746744 3 CTCAATC 25385 0.0 8.680269 2 GTTACCG 6195 0.0 8.59746 7 GGTAATC 7355 0.0 8.321953 2 TTACCGA 6330 0.0 8.300743 8 >>END_MODULE skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6031373 SRR6031373_1.fastq SRR6031373_2.fastq Input file: SRR6031373_1.fastq Paired file: SRR6031373_2.fastq trimmed: SRR6031373-trimmed-pair1.fastq, SRR6031373-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Apr 15 04:26:04 2025 >> started Tue Apr 15 04:26:35 2025 >> done (31.385s) 20486938 read pairs processed; of these: 78 ( 0.00%) short read pairs filtered out after trimming by size control 5896 ( 0.03%) empty read pairs filtered out after trimming by size control 20480964 (99.97%) read pairs available; of these: 927226 ( 4.53%) trimmed read pairs available after processing 19553738 (95.47%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 3 0.00% 20 6 0.00% 21 9 0.00% 22 2 0.00% 23 3 0.00% 24 9 0.00% 25 5 0.00% 26 3 0.00% 27 6 0.00% 28 5 0.00% 29 3 0.00% 30 20 0.00% 31 10 0.00% 32 4 0.00% 33 13 0.00% 34 8 0.00% 35 10 0.00% 36 6 0.00% 37 9 0.00% 38 9 0.00% 39 13 0.00% 40 7 0.00% 41 15 0.00% 42 8 0.00% 43 12 0.00% 44 19 0.00% 45 20 0.00% 46 9 0.00% 47 27 0.00% 48 23 0.00% 49 24 0.00% 50 35 0.00% 51 17 0.00% 52 35 0.00% 53 50 0.00% 54 48 0.00% 55 49 0.00% 56 44 0.00% 57 59 0.00% 58 71 0.00% 59 68 0.00% 60 81 0.00% 61 91 0.00% 62 95 0.00% 63 139 0.00% 64 130 0.00% 65 122 0.00% 66 150 0.00% 67 170 0.00% 68 199 0.00% 69 198 0.00% 70 232 0.00% 71 284 0.00% 72 317 0.00% 73 401 0.00% 74 423 0.00% 75 450 0.00% 76 483 0.00% 77 559 0.00% 78 612 0.00% 79 693 0.00% 80 760 0.00% 81 876 0.00% 82 1014 0.00% 83 1119 0.01% 84 1273 0.01% 85 1336 0.01% 86 1571 0.01% 87 1596 0.01% 88 1745 0.01% 89 1980 0.01% 90 2030 0.01% 91 2301 0.01% 92 2538 0.01% 93 2862 0.01% 94 3192 0.02% 95 3325 0.02% 96 3512 0.02% 97 3815 0.02% 98 4095 0.02% 99 4291 0.02% 100 4542 0.02% 101 4887 0.02% 102 5397 0.03% 103 5760 0.03% 104 6268 0.03% 105 6487 0.03% 106 6895 0.03% 107 7085 0.03% 108 7445 0.04% 109 7750 0.04% 110 8262 0.04% 111 8608 0.04% 112 9102 0.04% 113 9487 0.05% 114 10177 0.05% 115 10544 0.05% 116 11157 0.05% 117 11843 0.06% 118 12174 0.06% 119 12520 0.06% 120 13138 0.06% 121 13384 0.07% 122 14099 0.07% 123 14796 0.07% 124 15799 0.08% 125 16406 0.08% 126 17065 0.08% 127 17767 0.09% 128 18004 0.09% 129 19092 0.09% 130 19284 0.09% 131 19841 0.10% 132 21025 0.10% 133 21518 0.11% 134 22469 0.11% 135 23305 0.11% 136 23997 0.12% 137 24978 0.12% 138 25819 0.13% 139 26737 0.13% 140 27301 0.13% 141 28258 0.14% 142 29420 0.14% 143 30141 0.15% 144 31330 0.15% 145 32344 0.16% 146 33434 0.16% 147 34602 0.17% 148 35883 0.18% 149 37738 0.18% 150 19553738 95.47% 20480964 reads passed initial QC criterion=sequence-density sequence-density=0.13 sequence-density-rank=1 fanout-score=11.85 fanout-score-rank=14 prefix-density=0.44 prefix-fanout=3.6 sequence=AAAGAAAGAAAAAGCCAAGAAACATGACTAGAGGCAATTGCAGCTAGCCTATTCCATCATTATTTAGCGCCAACACCCTTTCAGGCTTTCAGCATTCATATGGATGAACTTAAAGGCCCAACCTTCTTAAACAAAACCACGAAAGGTGACAGTTTATCACTAAGAGACAGAATTCTACCTCTATTATTCTCAAAATAAATCCCAATATCCCTGCATAAAACTCCGCAGTGACAAATGTCTTCAGGACAGAAAACTAGCTTGTACCCTAATGTGCCAGCCTTCTCAATCTTGAACCAGTTGGTTAATGTATGAACACCAGGATTTCCTTCTTCCCCACCCGTTGTCACAAACCATTGCACCTCCGAGTTGGAAGATTTCTGAATCTTCCAAACTGACGAGTGGTCACAGGCTTTCTTGATAGAAAACTTGATGTTAAGATCAGTAGAAACTCGGATGACATCATCTTCGGAGCTGGCAGGTGAGAAGGTAACTGGAAGACCTTGTAACTGGT criterion=fanout-score sequence-density=0.07 sequence-density-rank=22 fanout-score=422.76 fanout-score-rank=1 prefix-density=0.78 prefix-fanout=36.1 sequence=CTTCTTCTTCCT criterion=sequence-density sequence-density=0.16 sequence-density-rank=1 fanout-score=2.39 fanout-score-rank=34 prefix-density=0.17 prefix-fanout=2.2 sequence=TGCCGTTCATGCTGAAGCAGTGATCGATG criterion=fanout-score sequence-density=0.02 sequence-density-rank=33 fanout-score=95.76 fanout-score-rank=1 prefix-density=0.12 prefix-fanout=11.9 sequence=CAAAGGAAATGTTACCGACAAAGCTAGCCGTCCCTCTTCTAATTCTTCTCTTCT SRR6031373 testing PE reads STAR mapping to Ensembl genome Started job on | Apr 15 04:27:20 Started mapping on | Apr 15 04:27:20 Finished on | Apr 15 04:30:07 Mapping speed, Million of reads per hour | 441.51 Number of input reads | 20480964 Average input read length | 298 UNIQUE READS: Uniquely mapped reads number | 19138264 Uniquely mapped reads % | 93.44% Average mapped length | 296.95 Number of splices: Total | 18583858 Number of splices: Annotated (sjdb) | 18264955 Number of splices: GT/AG | 18274017 Number of splices: GC/AG | 250398 Number of splices: AT/AC | 11918 Number of splices: Non-canonical | 47525 Mismatch rate per base, % | 0.35% Deletion rate per base | 0.03% Deletion average length | 2.72 Insertion rate per base | 0.02% Insertion average length | 2.12 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 671604 % of reads mapped to multiple loci | 3.28% Number of reads mapped to too many loci | 23294 % of reads mapped to too many loci | 0.11% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.13% % of reads unmapped: other | 0.03% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 671096 671096 671096 N_multimapping 671604 671604 671604 N_noFeature 487974 18916381 601472 N_ambiguous 241709 1188 132556 UnstrandedReadsAssigned:18408581 PositiveStrandReadsAssigned:220695 NegativeStrandReadsAssigned:18404236 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 SRR6031373 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR6031373-trimmed-pair1.fastq SRR6031373-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,480,964 reads, 18,525,187 reads pseudoaligned [quant] estimated average fragment length: 271.843 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,156 rounds 52401 SRR6031373.ke.tsv 34699 SRR6031373.se.tsv 87100 total ==> SRR6031373.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1747.16 525 15.9917 Potri.005G024800.1.v4.1 1035 764.157 151 10.5163 Potri.004G059700.1.v4.1 961 690.261 6 0.462599 Potri.007G009000.2.v4.1 1416 1145.16 0 0 Potri.003G141000.2.v4.1 2943 2672.16 679.158 13.5262 Potri.016G087400.1.v4.1 270 68.74 1320 1021.96 Potri.015G069301.1.v4.1 564 303.436 0 0 Potri.010G195200.1.v4.1 1773 1502.16 47 1.66514 Potri.012G127500.1.v4.1 977 706.205 3326 250.645 ==> SRR6031373.se.tsv <== Potri.001G166300.v4.1 1 Potri.001G448400.v4.1 95 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 334 Potri.001G212900.v4.1 14 Potri.001G182400.v4.1 3 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 9 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 26 SRR6031373 completed mapping pipeline successfully