Starting /dee2/code/volunteer_pipeline.sh SRR7166130 current disk space = 2796456747008 free memory = 1560121424 SRR7166130_1.fastq is conventional basespace SRR7166130_1.fastq read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7166130_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20161664 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0 30.0 30.0 30.0 30.0 30.0 2 30.0 30.0 30.0 30.0 30.0 30.0 3 30.0 30.0 30.0 30.0 30.0 30.0 4 30.0 30.0 30.0 30.0 30.0 30.0 5 30.0 30.0 30.0 30.0 30.0 30.0 6 30.0 30.0 30.0 30.0 30.0 30.0 7 30.0 30.0 30.0 30.0 30.0 30.0 8 30.0 30.0 30.0 30.0 30.0 30.0 9 30.0 30.0 30.0 30.0 30.0 30.0 10-14 30.0 30.0 30.0 30.0 30.0 30.0 15-19 30.0 30.0 30.0 30.0 30.0 30.0 20-24 30.0 30.0 30.0 30.0 30.0 30.0 25-29 30.0 30.0 30.0 30.0 30.0 30.0 30-34 30.0 30.0 30.0 30.0 30.0 30.0 35-39 30.0 30.0 30.0 30.0 30.0 30.0 40-44 30.0 30.0 30.0 30.0 30.0 30.0 45-49 30.0 30.0 30.0 30.0 30.0 30.0 50-54 30.0 30.0 30.0 30.0 30.0 30.0 55-59 30.0 30.0 30.0 30.0 30.0 30.0 60-64 30.0 30.0 30.0 30.0 30.0 30.0 65-69 30.0 30.0 30.0 30.0 30.0 30.0 70-74 30.0 30.0 30.0 30.0 30.0 30.0 75-79 30.0 30.0 30.0 30.0 30.0 30.0 80-84 30.0 30.0 30.0 30.0 30.0 30.0 85-89 30.0 30.0 30.0 30.0 30.0 30.0 90-94 30.0 30.0 30.0 30.0 30.0 30.0 95-99 30.0 30.0 30.0 30.0 30.0 30.0 100-104 30.0 30.0 30.0 30.0 30.0 30.0 105-109 30.0 30.0 30.0 30.0 30.0 30.0 110-114 30.0 30.0 30.0 30.0 30.0 30.0 115-119 30.0 30.0 30.0 30.0 30.0 30.0 120-124 30.0 30.0 30.0 30.0 30.0 30.0 125-129 30.0 30.0 30.0 30.0 30.0 30.0 130-134 30.0 30.0 30.0 30.0 30.0 30.0 135-139 30.0 30.0 30.0 30.0 30.0 30.0 140-144 30.0 30.0 30.0 30.0 30.0 30.0 145-149 30.0 30.0 30.0 30.0 30.0 30.0 150-151 30.0 30.0 30.0 30.0 30.0 30.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 2.0161664E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.04531651913397 20.347868828718234 14.982893466523608 27.62392118562419 2 21.48340299127823 24.942823280773887 36.71389419944781 16.859879528500073 3 17.324444899874926 33.202642861319816 29.22965118946466 20.243261049340596 4 19.32192699967622 37.3508258048542 25.421240032568743 17.90600716290084 5 19.575764703186422 37.467183747651355 26.088085470238937 16.868966078923282 6 15.987971032549694 38.24357949820015 26.032876056262023 19.735573412988135 7 12.747742448242366 21.71971023820256 45.98378387815609 19.548763435398982 8 17.51247331080265 23.89392878327417 28.67113328853121 29.92246461739197 9 18.00839405130302 23.79510535318547 31.286592040863304 26.909908554648204 10-14 19.10085695307689 32.48406778329408 26.022396762489446 22.39267850113959 15-19 19.219849115628552 31.256584773955165 27.118788409528104 22.404777700888182 20-24 19.164083877203787 31.229874677010788 27.4909074965241 22.11513394926133 25-29 19.44382442832516 31.079237107742042 27.325880898847714 22.151057565085086 30-34 19.56565765863999 31.264491115990868 26.946067624024195 22.22378360134495 35-39 20.25594038523745 31.14320555231161 26.713759605494463 21.887094456956476 40-44 20.09769056326947 31.239526656454807 26.76862017240167 21.894162607874048 45-49 20.250284072139145 30.96690378530714 26.66296633812114 22.11984580443258 50-54 19.65236982135414 31.216657495151956 26.565619835813013 22.565352847680888 55-59 19.44601728335099 31.316756423046144 26.711184647616935 22.52604164598593 60-64 19.335933710845644 30.783881311276893 27.076820592975025 22.803364384902434 65-69 19.92199116159303 30.637903158117197 26.784683177551376 22.655422502738396 70-74 20.152056814698273 30.586202615623225 26.816699647979807 22.445040921698695 75-79 20.086860004516232 30.69530590120601 26.470571542485093 22.747262551792666 80-84 20.25081312991351 30.144054471300763 26.925110890732096 22.680021508053635 85-89 20.290944914210655 29.942448630438516 27.337825661330324 22.428780794020504 90-94 20.04913698083201 30.322672100017446 27.42350451720266 22.204686401947892 95-99 20.479312297177014 29.95700468416861 27.010460867081775 22.5532221515726 100-104 20.46591963797758 29.899515077391705 26.578580121885786 23.05598516274493 105-109 20.412705445862517 29.969462085890896 26.472918754357654 23.144913713888933 110-114 20.4829733654219 30.044693796574844 26.230656816415348 23.241676021587907 115-119 20.762568518299844 30.06144386047837 25.973493277013105 23.20249434420868 120-124 21.213858481154265 29.77970199693652 26.022027498698453 22.984412023210762 125-129 21.00062432071304 29.954632800676684 26.135403990582915 22.90933888802736 130-134 21.22914283483697 30.160367640140333 25.538832761104885 23.071656763917808 135-139 21.022146624947588 30.098845342309062 25.658780056944103 23.220227975799247 140-144 21.361831415826522 29.76266379892694 25.27288483371528 23.60261995153125 145-149 21.515555903344993 29.924982836604435 24.78048690671307 23.7789743533375 150-151 21.538584576568077 29.18683668879252 24.75689679439593 24.51768194024347 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3586.0 1 2605.5 2 1491.5 3 1309.0 4 1298.5 5 1354.5 6 1356.5 7 1351.0 8 1388.0 9 1412.5 10 1433.5 11 1463.5 12 1501.5 13 1628.0 14 1870.0 15 2766.5 16 3638.5 17 4231.0 18 5941.0 19 8402.5 20 9510.5 21 11391.0 22 15620.0 23 21373.0 24 29100.5 25 40367.0 26 59944.0 27 87800.0 28 118616.5 29 168963.0 30 251176.0 31 345089.5 32 444840.5 33 567757.0 34 672711.5 35 796749.0 36 928838.0 37 974473.0 38 1017346.0 39 1021189.0 40 950670.0 41 895242.0 42 870702.5 43 862485.5 44 864756.5 45 870650.5 46 871370.0 47 819102.5 48 758720.5 49 726620.5 50 659147.0 51 581123.5 52 543165.0 53 495966.5 54 390948.0 55 297329.0 56 228540.5 57 187108.0 58 159520.0 59 124326.0 60 92395.5 61 66662.0 62 54359.0 63 48167.5 64 34039.0 65 18327.0 66 11280.0 67 8793.0 68 8704.0 69 7615.5 70 5411.5 71 3365.5 72 2645.5 73 3081.0 74 2657.5 75 1945.5 76 1745.5 77 880.5 78 545.5 79 336.0 80 78.0 81 23.5 82 12.5 83 4.0 84 1.5 85 1.5 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.061230065137480714 2 2.4303549548291252E-4 3 1.5375715020347528E-4 4 0.0 5 0.002683310266454197 6 0.0 7 0.0 8 0.0020682816656402963 9 2.4799540355399237E-5 10-14 0.0 15-19 0.0 20-24 0.0 25-29 8.927834527943724E-6 30-34 3.967926456863878E-6 35-39 9.919816142159695E-7 40-44 1.587170582745551E-5 45-49 0.006408201227835162 50-54 0.039476900319338724 55-59 0.06988312075828662 60-64 0.037265773301251325 65-69 0.0391654180924749 70-74 0.03230586523017148 75-79 0.025023728200212043 80-84 0.01617723616463403 85-89 0.010460446121907397 90-94 0.007600563128122758 95-99 0.01189485153606369 100-104 0.01970670674801445 105-109 0.042742503793337695 110-114 0.010133092189216127 115-119 0.01505232901411312 120-124 0.00914805444629967 125-129 0.012269820586237327 130-134 0.0021178807463510946 135-139 0.0010326528603988242 140-144 0.0023142931059658566 145-149 0.006535174874454807 150-151 0.02632223213322075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 2.0161664E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.856457473819916 #Duplication Level Percentage of deduplicated Percentage of total 1 51.984850655377315 12.401743789428881 2 20.648851325210156 9.852168870462123 3 9.729510882006858 6.96334987792994 4 5.000748521333294 4.772005777458221 5 2.9005393618990807 3.4598296969143085 6 1.9388308352707335 2.7752181222340204 7 1.316965605035293 2.1992693765705584 8 0.975885375872767 1.8624934375065145 9 0.7508689091088679 1.6121764978712336 >10 4.222057721548995 18.405338010895335 >50 0.3035037100285666 4.994497998601208 >100 0.18228906747674067 8.48252967343988 >500 0.022069535343688825 3.6814400936465956 >1k 0.019336136688277484 9.115862010811103 >5k 0.0025514607263071015 4.17823336182627 >10k+ 0.001140897072738948 5.243843404403865 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 52151 0.2586641658148851 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 47028 0.23325455676674306 No Hit GATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATG 38027 0.18861042421895335 No Hit GTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATAC 37046 0.18374475440122404 No Hit AAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAA 35092 0.174053094030334 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 35019 0.17369102074114517 No Hit TGGCACTCCAATGATTCCATATAACGGCCATAATGGAGCTATAGAATACA 30619 0.15186742522839386 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 29151 0.14458628018004863 No Hit GTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTA 28614 0.14192280954587877 No Hit GTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTAGAATTACTGG 27598 0.13688354294566163 No Hit TGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATA 27555 0.1366702668986052 No Hit CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT 25660 0.1272712411039089 No Hit GCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGC 24894 0.12347195152146173 No Hit GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT 24379 0.1209175988648556 No Hit GGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTA 23757 0.11783253604464394 No Hit CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC 22420 0.11120113895361017 No Hit CTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCAC 21976 0.10899893977005072 No Hit GAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAATATGACA 20529 0.10182195279119818 No Hit GCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAA 20244 0.10040837899094043 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0011209392240640456 0.0 0.0 0.0 0.0 2 0.0011606184886326842 0.0 0.0 0.0 0.0 3 0.0011953378451302432 0.0 0.0 0.0 0.0 4 0.0012300572016278022 0.0 0.0 0.0 0.0 5 0.001235017109698882 0.0 0.0 0.0 0.0 6 0.0012548567419832014 0.0 0.0 0.0 0.0 7 0.0012598166500542812 0.0 0.0 0.0 0.0 8 0.0012697364661964409 0.0 0.0 0.0 0.0 9 0.0012846161904096806 0.0 0.0 0.0 0.0 10-11 0.001329255363049399 0.0 0.0 0.0 0.0 12-13 0.0013565348574403383 0.0 0.0 0.0 0.0 14-15 0.0013862943058668173 0.0 0.0 0.0 0.0 16-17 0.001401174030080057 0.0 0.0 0.0 0.0 18-19 0.0014160537542932965 0.0 0.0 4.959908071079847E-6 0.0 20-21 0.0014334134325420759 0.0 0.0 4.959908071079847E-6 0.0 22-23 0.0014755726511462546 0.0 0.0 4.959908071079847E-6 0.0 24-25 0.0015053320995727336 0.0 0.0 9.919816142159694E-6 0.0 26-27 0.0015499712722124523 0.0 0.0 9.919816142159694E-6 0.0 28-29 0.001594610444852171 0.0 0.0 9.919816142159694E-6 0.0 30-31 0.001659089249776209 0.0 0.0 9.919816142159694E-6 0.0 32-33 0.0017012484683803877 0.0 0.0 1.4879724213239541E-5 0.0 34-35 0.001758287411197806 2.4799540355399236E-6 0.0 1.4879724213239541E-5 0.0 36-37 0.0018153263540152242 4.959908071079847E-6 0.0 1.4879724213239541E-5 0.0 38-39 0.001889724975081422 1.983963228431939E-5 0.0 1.4879724213239541E-5 2.4799540355399236E-6 40-41 0.002031082355107197 1.983963228431939E-5 0.0 1.4879724213239541E-5 4.959908071079847E-6 42-43 0.0021773996432040534 1.983963228431939E-5 0.0 1.4879724213239541E-5 4.959908071079847E-6 44-45 0.0024725141734333036 1.983963228431939E-5 0.0 1.4879724213239541E-5 4.959908071079847E-6 46-47 0.0028519471408709122 1.983963228431939E-5 0.0 2.727949439093916E-5 4.959908071079847E-6 48-49 0.003370257534298756 1.983963228431939E-5 0.0 3.471935649755893E-5 4.959908071079847E-6 50-51 0.0040398451238945355 1.983963228431939E-5 0.0 3.471935649755893E-5 4.959908071079847E-6 52-53 0.004945028346866608 4.4639172639718626E-5 0.0 3.471935649755893E-5 4.959908071079847E-6 54-55 0.006127966421819151 4.9599080710798475E-5 0.0 3.471935649755893E-5 4.959908071079847E-6 56-57 0.007625858659285265 4.9599080710798475E-5 0.0 3.471935649755893E-5 4.959908071079847E-6 58-59 0.009726379727387581 4.9599080710798475E-5 0.0 3.967926456863878E-5 4.959908071079847E-6 60-61 0.012417129855948398 5.4558988781878324E-5 0.0 3.967926456863878E-5 4.959908071079847E-6 62-63 0.01617922012786246 5.7038942817418245E-5 0.0 3.967926456863878E-5 4.959908071079847E-6 64-65 0.02174423698361405 5.9518896852958166E-5 0.0 3.967926456863878E-5 4.959908071079847E-6 66-67 0.028755067042085414 5.9518896852958166E-5 0.0 4.4639172639718626E-5 4.959908071079847E-6 68-69 0.037231549935560874 5.9518896852958166E-5 0.0 5.4558988781878324E-5 4.959908071079847E-6 70-71 0.049165088754578985 5.9518896852958166E-5 0.0 5.4558988781878324E-5 4.959908071079847E-6 72-73 0.06634869026683513 6.199885088849809E-5 0.0 6.447880492403801E-5 4.959908071079847E-6 74-75 0.09004713103045463 6.447880492403801E-5 0.0 8.431843720835741E-5 4.959908071079847E-6 76-77 0.11952386469688217 6.447880492403801E-5 0.0 9.42382533505171E-5 4.959908071079847E-6 78-79 0.1549772875889609 6.447880492403801E-5 0.0 9.42382533505171E-5 7.439862106619771E-6 80-81 0.20020916924317358 6.695875895957793E-5 0.0 9.919816142159695E-5 9.919816142159694E-6 82-83 0.2627684897437037 6.943871299511786E-5 0.0 9.919816142159695E-5 9.919816142159694E-6 84-85 0.34701252833099494 7.439862106619771E-5 0.0 9.919816142159695E-5 9.919816142159694E-6 86-87 0.44561302083002674 7.439862106619771E-5 0.0 1.0415806949267679E-4 9.919816142159694E-6 88-89 0.5597206659132897 7.439862106619771E-5 0.0 1.0415806949267679E-4 9.919816142159694E-6 90-91 0.6958056636595075 7.935852913727755E-5 0.0 1.0415806949267679E-4 9.919816142159694E-6 92-93 0.8648194910896243 9.175829931497718E-5 0.0 1.0415806949267679E-4 9.919816142159694E-6 94-95 1.0765331671036678 9.919816142159695E-5 0.0 1.0415806949267679E-4 9.919816142159694E-6 96-97 1.3201539317389677 9.919816142159695E-5 0.0 1.0663802352821672E-4 9.919816142159694E-6 98-99 1.578671780265756 1.0911797756375665E-4 0.0 1.0911797756375665E-4 9.919816142159694E-6 100-101 1.860627674382432 1.2647765581253611E-4 0.0 1.0911797756375665E-4 9.919816142159694E-6 102-103 2.1840831193298333 1.388774259902357E-4 0.0 1.0911797756375665E-4 9.919816142159694E-6 104-105 2.561718120091675 1.388774259902357E-4 0.0 1.1159793159929658E-4 9.919816142159694E-6 106-107 2.984210529448363 1.388774259902357E-4 0.0 1.1407788563483649E-4 9.919816142159694E-6 108-109 3.4191870274199587 1.388774259902357E-4 0.0 1.1407788563483649E-4 9.919816142159694E-6 110-111 3.863532791737825 1.388774259902357E-4 0.0 1.239977017769962E-4 1.4879724213239541E-5 112-113 4.346419521722017 1.388774259902357E-4 0.0 1.3391751791915588E-4 1.4879724213239541E-5 114-115 4.91988905280834 1.6863687441671482E-4 0.0 1.3391751791915588E-4 1.4879724213239541E-5 116-117 5.525630225759143 1.6863687441671482E-4 0.0 1.3391751791915588E-4 1.4879724213239541E-5 118-119 6.133486799502263 1.7111682845225473E-4 0.0 1.3391751791915588E-4 1.983963228431939E-5 120-121 6.73898741691162 1.7607673652333456E-4 0.0 1.3391751791915588E-4 1.983963228431939E-5 122-123 7.382185815615219 1.8351659862995436E-4 0.0 1.363974719546958E-4 1.983963228431939E-5 124-125 8.087531862449449 1.8351659862995436E-4 0.0 1.388774259902357E-4 1.983963228431939E-5 126-127 8.851074990635695 1.9343641477211407E-4 0.0 1.388774259902357E-4 1.983963228431939E-5 128-129 9.610422036593805 2.0583618494981367E-4 0.0 1.388774259902357E-4 1.983963228431939E-5 130-131 10.373756848641065 2.1327604705643344E-4 0.0 1.388774259902357E-4 1.983963228431939E-5 132-133 11.15076116733222 2.1327604705643344E-4 0.0 1.4135738002577565E-4 1.983963228431939E-5 134-135 11.982247596230152 2.1327604705643344E-4 0.0 1.4383733406131557E-4 1.983963228431939E-5 136-137 12.859335419933593 2.1327604705643344E-4 0.0 1.4383733406131557E-4 1.983963228431939E-5 138-139 13.742771925968015 2.2567581723413306E-4 0.0 1.4383733406131557E-4 1.983963228431939E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCGCT 4820 0.0 36.26314 1 TTTTTTG 51885 0.0 27.18513 3 TTTTTTA 66860 0.0 24.684654 4 TTTTACG 7650 0.0 24.539244 6 TTACGTT 6230 0.0 24.431723 8 GCTGTCG 12600 0.0 23.182747 3 TACGTTT 6765 0.0 23.141962 9 CGGCTGT 13095 0.0 22.707733 1 CTGTCGA 13350 0.0 22.586164 4 GTCGAGT 14360 0.0 21.501959 6 TTTTTTT 430940 0.0 21.456297 1 TGTCGAG 14465 0.0 21.446465 5 TTTACGT 8830 0.0 21.01368 7 TAAGGGT 10995 0.0 19.57871 8 GTCGAAT 3600 0.0 19.541803 1 GACGGTC 9700 0.0 19.440664 145 TTAATCC 23085 0.0 18.46196 3 GTCGGAT 5115 0.0 18.149294 1 GCGGGAT 2850 0.0 18.067951 1 CGCTCTT 10115 0.0 18.057808 4 >>END_MODULE SRR7166130 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7166130_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20161664 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0 30.0 30.0 30.0 30.0 30.0 2 30.0 30.0 30.0 30.0 30.0 30.0 3 30.0 30.0 30.0 30.0 30.0 30.0 4 30.0 30.0 30.0 30.0 30.0 30.0 5 30.0 30.0 30.0 30.0 30.0 30.0 6 30.0 30.0 30.0 30.0 30.0 30.0 7 30.0 30.0 30.0 30.0 30.0 30.0 8 30.0 30.0 30.0 30.0 30.0 30.0 9 30.0 30.0 30.0 30.0 30.0 30.0 10-14 30.0 30.0 30.0 30.0 30.0 30.0 15-19 30.0 30.0 30.0 30.0 30.0 30.0 20-24 30.0 30.0 30.0 30.0 30.0 30.0 25-29 30.0 30.0 30.0 30.0 30.0 30.0 30-34 30.0 30.0 30.0 30.0 30.0 30.0 35-39 30.0 30.0 30.0 30.0 30.0 30.0 40-44 30.0 30.0 30.0 30.0 30.0 30.0 45-49 30.0 30.0 30.0 30.0 30.0 30.0 50-54 30.0 30.0 30.0 30.0 30.0 30.0 55-59 30.0 30.0 30.0 30.0 30.0 30.0 60-64 30.0 30.0 30.0 30.0 30.0 30.0 65-69 30.0 30.0 30.0 30.0 30.0 30.0 70-74 30.0 30.0 30.0 30.0 30.0 30.0 75-79 30.0 30.0 30.0 30.0 30.0 30.0 80-84 30.0 30.0 30.0 30.0 30.0 30.0 85-89 30.0 30.0 30.0 30.0 30.0 30.0 90-94 30.0 30.0 30.0 30.0 30.0 30.0 95-99 30.0 30.0 30.0 30.0 30.0 30.0 100-104 30.0 30.0 30.0 30.0 30.0 30.0 105-109 30.0 30.0 30.0 30.0 30.0 30.0 110-114 30.0 30.0 30.0 30.0 30.0 30.0 115-119 30.0 30.0 30.0 30.0 30.0 30.0 120-124 30.0 30.0 30.0 30.0 30.0 30.0 125-129 30.0 30.0 30.0 30.0 30.0 30.0 130-134 30.0 30.0 30.0 30.0 30.0 30.0 135-139 30.0 30.0 30.0 30.0 30.0 30.0 140-144 30.0 30.0 30.0 30.0 30.0 30.0 145-149 30.0 30.0 30.0 30.0 30.0 30.0 150-151 30.0 30.0 30.0 30.0 30.0 30.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 2.0161664E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.91833074889057 17.175462303111487 14.464232714125183 26.441974233872763 2 25.507527553281317 22.579138309218923 33.827882460495324 18.08545167700444 3 21.52817444217055 25.50616580973931 32.84345459938357 20.122205148706573 4 24.75479718014339 34.62858077610289 21.865133104646176 18.751488939107546 5 24.144499422013023 36.074343022795 21.48802605221852 18.293131502973463 6 19.38548484648383 36.71277093923119 23.28918575831779 20.612558455967186 7 17.644092760202774 16.68125163541342 43.54876352574345 22.12589207864035 8 21.391587492888885 21.75333104362042 26.797559000003023 30.05752246348767 9 22.172632742924876 23.29507148093814 28.88574297205691 25.64655280408007 10-14 23.01368150347872 27.902899553940347 26.98006677020302 22.103352172377917 15-19 24.08463085331197 27.131743685239844 28.075846399259476 20.707779062188706 20-24 24.06332328973326 27.382456126561312 27.753688831243206 20.800531752462227 25-29 23.996266853275053 27.216699241376897 28.031032600719197 20.756001304628853 30-34 23.29403804672331 26.840535219691287 28.703677128485428 21.161749605099974 35-39 23.515758693251104 27.033046744873353 28.76070166287205 20.690492899003495 40-44 23.80364629479287 27.27067443097837 28.750428695590198 20.175250578638565 45-49 22.82082368013823 26.600952335985788 29.794592659740204 20.783631324135776 50-54 22.76105259812286 26.760041170969323 29.44907693177216 21.029829299135656 55-59 23.283038404477683 26.65178573732637 29.65263442194462 20.412541436251328 60-64 22.557187779441286 27.518402492955452 29.440798729701495 20.483610997901767 65-69 22.584088821879327 27.579225807470998 29.00037472544186 20.836310645207814 70-74 22.392004458489843 27.554057665606376 29.322838613823244 20.731099262080544 75-79 22.596147150511765 27.37378287840017 29.26953163616567 20.76053833492239 80-84 22.753575415426912 27.567126510702185 29.517488810850427 20.16180926302048 85-89 22.752075518988317 27.98207218128329 29.351411700331166 19.914440599397228 90-94 22.96673383212339 27.921006006020015 29.370221652757017 19.742038509099576 95-99 23.128923926355053 27.85135413576767 28.986381749554425 20.03334018832285 100-104 22.91035405261459 27.6787826701508 28.956089826750464 20.454773450484147 105-109 23.248179975526863 27.886430909648425 28.954469781970303 19.91091933285441 110-114 22.99207233361982 28.44749124533798 28.6192766620146 19.941159759027602 115-119 23.484709790035147 28.461952176528925 28.680415175243713 19.37292285819222 120-124 23.73272593505466 27.98354362848633 28.428884767271274 19.85484566918774 125-129 24.041601002675392 28.06489778503422 28.668926727466626 19.22457448482376 130-134 24.448542587494103 27.722017502062034 28.57009045360204 19.259349456841818 135-139 24.71093635038764 27.933299436634883 28.229309427972122 19.12645478500535 140-144 25.453277878258877 27.663392122264497 28.058200294452025 18.825129705024597 145-149 25.9936733307543 27.59943046873162 27.7295901830805 18.67730601743358 150-151 26.8258920855569 26.976358235907494 27.47703718547897 18.720712493056634 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 240.0 1 255.5 2 368.5 3 544.5 4 687.5 5 747.5 6 822.0 7 881.0 8 882.0 9 884.0 10 848.5 11 829.5 12 859.5 13 926.0 14 997.0 15 1751.0 16 2074.0 17 2171.0 18 3039.5 19 3370.5 20 3507.0 21 4693.5 22 6707.0 23 8595.0 24 9641.0 25 12205.5 26 20320.0 27 31587.5 28 48242.0 29 75350.5 30 133276.5 31 196438.5 32 249072.0 33 304744.5 34 391659.0 35 494387.0 36 585369.0 37 753378.5 38 968906.0 39 1038782.0 40 981810.5 41 927241.0 42 941888.5 43 990847.0 44 1022295.5 45 1057293.5 46 1059552.0 47 1018917.0 48 972845.5 49 898026.5 50 778066.5 51 674925.5 52 625303.5 53 603560.0 54 533497.0 55 400719.5 56 308341.5 57 241590.0 58 186544.5 59 162020.0 60 120926.5 61 78398.5 62 51967.5 63 46544.0 64 35176.5 65 20262.0 66 12537.5 67 10092.5 68 8953.5 69 7413.5 70 5607.5 71 3955.5 72 3111.5 73 2873.0 74 2123.5 75 1613.0 76 1399.0 77 1364.5 78 1132.5 79 406.0 80 162.0 81 86.0 82 57.0 83 46.0 84 38.5 85 32.5 86 26.5 87 24.5 88 25.5 89 22.5 90 14.5 91 12.5 92 13.0 93 8.5 94 5.0 95 5.5 96 6.0 97 4.5 98 3.0 99 1.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.002400595506402646 4 0.002465074311326684 5 0.0015375715020347527 6 0.026054397097382435 7 0.03080102912140585 8 0.0240208347882397 9 0.028425233155358606 10-14 0.012063488410480406 15-19 0.010621147143410385 20-24 0.04527999276250214 25-29 0.022387041069626 30-34 0.04573828826826992 35-39 0.046732253845714326 40-44 0.04299843504980541 45-49 0.055821781376775244 50-54 0.063036463656968 55-59 0.05431694526800963 60-64 0.0554894675360129 65-69 0.05146797407198136 70-74 0.06872151028803972 75-79 0.06773548056350905 80-84 0.053580894910261384 85-89 0.06492023674236412 90-94 0.06454427571057628 95-99 0.0829633903233384 100-104 0.06833364547688128 105-109 0.06873440604902453 110-114 0.09181087434053062 115-119 0.12299877629148069 120-124 0.14654941179458203 125-129 0.11043136122097859 130-134 0.09042805197031356 135-139 0.06055551763981385 140-144 0.05866480068311822 145-149 0.07226586059563338 150-151 0.032896590281437085 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 2.0161664E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.76923840470084 #Duplication Level Percentage of deduplicated Percentage of total 1 54.117771665417955 13.945737599773194 2 20.001869268142876 10.308658754208658 3 9.438770332751538 7.296897688556757 4 4.8517044429279945 5.000989138358312 5 2.7215853968038664 3.506659146449559 6 1.7676097528467907 2.7329974276549964 7 1.2619610795748553 2.2763843141912665 8 0.8870684375821367 1.8287262439471657 9 0.6985324202939033 1.6200583624769653 >10 3.807919847141102 17.59714630808358 >50 0.2552854738842784 4.525346498629391 >100 0.1514093788970343 7.709623088529726 >500 0.01867119236480844 3.306260515687498 >1k 0.01676259547084609 8.526550573439897 >5k 0.0020781332321520808 3.649260244811158 >10k+ 0.0010005826673324832 6.1687040952019725 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 142040 0.7045053424161815 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 88228 0.4376027692952328 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 61944 0.30723654555497004 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 51237 0.25413080983791814 No Hit CTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCT 41922 0.20792926615580934 No Hit TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC 40817 0.20244856773726613 No Hit GTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATAT 35504 0.17609657615561888 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 34154 0.1694007002596611 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 30845 0.1529883644524579 No Hit CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA 28951 0.14359429856583267 No Hit GTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTGGAAAAGTC 28713 0.14241384044491567 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 28711 0.1424039206287735 No Hit ATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTACTATGT 27570 0.1367446655196714 No Hit AGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTACTA 26236 0.13012814815285087 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 25487 0.12641317700761207 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 24602 0.1220236583647064 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 23462 0.11636936316367537 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 21708 0.10766968440700134 No Hit GTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGG 21120 0.10475325846120637 No Hit ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC 20772 0.1030272104524706 No Hit TTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTACTATGTT 20769 0.10301233072825736 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0010961396837086462 0.0 0.0 0.0 0.0 2 0.001135818948277285 0.0 0.0 4.959908071079847E-6 0.0 3 0.0011457387644194447 0.0 0.0 4.959908071079847E-6 0.0 4 0.001165578396703764 0.0 0.0 9.919816142159694E-6 0.0 5 0.001170538304774844 0.0 0.0 1.4879724213239541E-5 0.0 6 0.001170538304774844 0.0 0.0 1.4879724213239541E-5 0.0 7 0.001170538304774844 0.0 0.0 1.4879724213239541E-5 0.0 8 0.001175498212845924 0.0 0.0 1.4879724213239541E-5 0.0 9 0.0011903779370591634 0.0 0.0 1.4879724213239541E-5 0.0 10-11 0.001202777707236863 0.0 0.0 1.4879724213239541E-5 9.919816142159694E-6 12-13 0.001237497063734422 0.0 0.0 1.4879724213239541E-5 9.919816142159694E-6 14-15 0.0012697364661964409 2.4799540355399236E-6 0.0 1.4879724213239541E-5 9.919816142159694E-6 16-17 0.0012920560525163003 4.959908071079847E-6 0.0 1.4879724213239541E-5 9.919816142159694E-6 18-19 0.0013094157307650797 4.959908071079847E-6 0.0 1.4879724213239541E-5 9.919816142159694E-6 20-21 0.0013267754090138591 4.959908071079847E-6 0.0 1.4879724213239541E-5 9.919816142159694E-6 22-23 0.0013515749493692585 4.959908071079847E-6 2.4799540355399236E-6 2.4799540355399234E-5 9.919816142159694E-6 24-25 0.0013738945356891177 4.959908071079847E-6 4.959908071079847E-6 3.471935649755893E-5 9.919816142159694E-6 26-27 0.0014210136623643763 4.959908071079847E-6 4.959908071079847E-6 3.471935649755893E-5 9.919816142159694E-6 28-29 0.0014706127430751748 4.959908071079847E-6 4.959908071079847E-6 3.471935649755893E-5 9.919816142159694E-6 30-31 0.0015177318697504332 4.959908071079847E-6 4.959908071079847E-6 3.471935649755893E-5 9.919816142159694E-6 32-33 0.0015574111343190722 4.959908071079847E-6 4.959908071079847E-6 3.471935649755893E-5 9.919816142159694E-6 34-35 0.0016045302609943306 4.959908071079847E-6 4.959908071079847E-6 3.471935649755893E-5 9.919816142159694E-6 36-37 0.001659089249776209 4.959908071079847E-6 9.919816142159694E-6 3.471935649755893E-5 9.919816142159694E-6 38-39 0.001721088100664707 4.959908071079847E-6 9.919816142159694E-6 3.471935649755893E-5 9.919816142159694E-6 40-41 0.001850045710512783 4.959908071079847E-6 9.919816142159694E-6 3.471935649755893E-5 9.919816142159694E-6 42-43 0.0019988429526451787 4.959908071079847E-6 1.7359678248779464E-5 4.4639172639718626E-5 9.919816142159694E-6 44-45 0.0022542782183057903 4.959908071079847E-6 1.983963228431939E-5 5.9518896852958166E-5 9.919816142159694E-6 46-47 0.002626271323636779 4.959908071079847E-6 2.4799540355399237E-5 6.199885088849809E-5 9.919816142159694E-6 48-49 0.0031396218089935436 4.959908071079847E-6 2.4799540355399237E-5 7.687857510173764E-5 9.919816142159694E-6 50-51 0.0038191292147314827 4.959908071079847E-6 2.4799540355399237E-5 8.431843720835741E-5 9.919816142159694E-6 52-53 0.004739192161916794 4.959908071079847E-6 2.4799540355399237E-5 8.431843720835741E-5 9.919816142159694E-6 54-55 0.005929570098975958 4.959908071079847E-6 2.4799540355399237E-5 8.679839124389732E-5 9.919816142159694E-6 56-57 0.007424982382406532 4.959908071079847E-6 2.4799540355399237E-5 9.175829931497718E-5 9.919816142159694E-6 58-59 0.009518063588402226 4.959908071079847E-6 2.4799540355399237E-5 9.919816142159695E-5 9.919816142159694E-6 60-61 0.012179054268536566 4.959908071079847E-6 2.4799540355399237E-5 9.919816142159695E-5 9.919816142159694E-6 62-63 0.01590890513798861 4.959908071079847E-6 2.4799540355399237E-5 9.919816142159695E-5 9.919816142159694E-6 64-65 0.02142928282110048 4.959908071079847E-6 2.4799540355399237E-5 9.919816142159695E-5 9.919816142159694E-6 66-67 0.028450032695714004 4.959908071079847E-6 2.4799540355399237E-5 9.919816142159695E-5 9.919816142159694E-6 68-69 0.03698851444007796 4.959908071079847E-6 2.4799540355399237E-5 1.0167811545713686E-4 9.919816142159694E-6 70-71 0.048887333902598515 4.959908071079847E-6 2.4799540355399237E-5 1.0415806949267679E-4 9.919816142159694E-6 72-73 0.06609325500117451 4.959908071079847E-6 2.727949439093916E-5 1.0415806949267679E-4 9.919816142159694E-6 74-75 0.08965777824687486 4.959908071079847E-6 2.9759448426479083E-5 1.0415806949267679E-4 9.919816142159694E-6 76-77 0.11914195177540901 4.959908071079847E-6 2.9759448426479083E-5 1.0415806949267679E-4 9.919816142159694E-6 78-79 0.15446393710360415 4.959908071079847E-6 2.9759448426479083E-5 1.0415806949267679E-4 9.919816142159694E-6 80-81 0.19947014294058268 4.959908071079847E-6 2.9759448426479083E-5 1.0415806949267679E-4 9.919816142159694E-6 82-83 0.26169218969227936 4.959908071079847E-6 2.9759448426479083E-5 1.165578396703764E-4 9.919816142159694E-6 84-85 0.3455592752661685 4.959908071079847E-6 2.9759448426479083E-5 1.3143756388361594E-4 9.919816142159694E-6 86-87 0.4433438628875077 4.959908071079847E-6 2.9759448426479083E-5 1.4631728809685548E-4 9.919816142159694E-6 88-89 0.5564669662186613 4.959908071079847E-6 2.9759448426479083E-5 1.4879724213239542E-4 9.919816142159694E-6 90-91 0.6917310991791153 4.959908071079847E-6 2.9759448426479083E-5 1.4879724213239542E-4 9.919816142159694E-6 92-93 0.8601224581463117 7.439862106619771E-6 2.9759448426479083E-5 1.5375715020347528E-4 9.919816142159694E-6 94-95 1.072304845473072 9.919816142159694E-6 2.9759448426479083E-5 1.5375715020347528E-4 1.4879724213239541E-5 96-97 1.3157619331420265 9.919816142159694E-6 2.9759448426479083E-5 1.5375715020347528E-4 1.4879724213239541E-5 98-99 1.5743888996463784 9.919816142159694E-6 2.9759448426479083E-5 1.5375715020347528E-4 1.4879724213239541E-5 100-101 1.8561835967507445 9.919816142159694E-6 2.9759448426479083E-5 1.5375715020347528E-4 1.4879724213239541E-5 102-103 2.1790413727755804 9.919816142159694E-6 2.9759448426479083E-5 1.587170582745551E-4 1.4879724213239541E-5 104-105 2.5565771753760007 9.919816142159694E-6 2.9759448426479083E-5 1.6367696634563496E-4 1.4879724213239541E-5 106-107 2.9802897221181746 9.919816142159694E-6 2.9759448426479083E-5 1.785566905588745E-4 1.4879724213239541E-5 108-109 3.415796930253376 9.919816142159694E-6 2.9759448426479083E-5 2.008762768787338E-4 1.4879724213239541E-5 110-111 3.8605593268492124 9.919816142159694E-6 2.9759448426479083E-5 2.0335623091427375E-4 1.4879724213239541E-5 112-113 4.344088364928609 9.919816142159694E-6 2.9759448426479083E-5 2.1575600109197335E-4 1.4879724213239541E-5 114-115 4.9173272602896265 9.919816142159694E-6 2.9759448426479083E-5 2.2815577126967298E-4 1.4879724213239541E-5 116-117 5.522267408086952 9.919816142159694E-6 2.9759448426479083E-5 2.2815577126967298E-4 1.4879724213239541E-5 118-119 6.127929222508618 9.919816142159694E-6 2.9759448426479083E-5 2.2815577126967298E-4 1.4879724213239541E-5 120-121 6.730364616730048 9.919816142159694E-6 2.9759448426479083E-5 2.2815577126967298E-4 1.4879724213239541E-5 122-123 7.371581532159251 9.919816142159694E-6 2.9759448426479083E-5 2.2815577126967298E-4 1.4879724213239541E-5 124-125 8.07498081507558 9.919816142159694E-6 2.9759448426479083E-5 2.2815577126967298E-4 1.4879724213239541E-5 126-127 8.836517660447074 9.919816142159694E-6 2.9759448426479083E-5 2.2815577126967298E-4 1.4879724213239541E-5 128-129 9.593724506072515 9.919816142159694E-6 2.9759448426479083E-5 2.2815577126967298E-4 1.4879724213239541E-5 130-131 10.354301609232255 9.919816142159694E-6 2.9759448426479083E-5 2.2815577126967298E-4 1.4879724213239541E-5 132-133 11.127943110251216 9.919816142159694E-6 2.9759448426479083E-5 2.306357253052129E-4 1.4879724213239541E-5 134-135 11.955813766165331 9.919816142159694E-6 2.9759448426479083E-5 2.3311567934075283E-4 1.4879724213239541E-5 136-137 12.828388073524088 9.919816142159694E-6 2.9759448426479083E-5 2.3311567934075283E-4 1.4879724213239541E-5 138-139 13.707392901697002 9.919816142159694E-6 2.9759448426479083E-5 2.4799540355399235E-4 1.4879724213239541E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCCCTA 53915 0.0 39.271423 4 AAAGCCC 54115 0.0 39.071365 2 GCCCTAA 55650 0.0 38.295025 5 CCCTAAC 56735 0.0 37.320004 6 GAAAGCC 58655 0.0 36.40542 1 AAGCCCT 58970 0.0 36.101624 3 GTCATAC 12880 0.0 35.891846 1 CCTAACT 59495 0.0 35.623924 7 TAACTTA 61440 0.0 34.400124 9 CTAACTT 65575 0.0 32.772354 8 TCGTAAC 6470 0.0 29.789852 2 CGTAACC 6320 0.0 29.580717 3 TCGTACG 1970 0.0 29.05808 4 GCTGAAA 46970 0.0 29.032875 1 CTGAAAG 46220 0.0 28.986643 2 GGAGTAT 30025 0.0 28.090595 1 TCATACT 16920 0.0 27.107803 2 CGTACGG 2135 0.0 26.812773 5 TGAAAGC 52185 0.0 26.243523 3 ACTTCCC 18335 0.0 24.58227 6 >>END_MODULE skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7166130 SRR7166130_1.fastq SRR7166130_2.fastq Input file: SRR7166130_1.fastq Paired file: SRR7166130_2.fastq trimmed: SRR7166130-trimmed-pair1.fastq, SRR7166130-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Apr 15 04:25:38 2025 >> started Tue Apr 15 04:26:05 2025 >> done (26.304s) 20161664 read pairs processed; of these: 78 ( 0.00%) short read pairs filtered out after trimming by size control 8562 ( 0.04%) empty read pairs filtered out after trimming by size control 20153024 (99.96%) read pairs available; of these: 4072512 (20.21%) trimmed read pairs available after processing 16080512 (79.79%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 1 0.00% 20 3 0.00% 21 4 0.00% 22 3 0.00% 23 2 0.00% 24 7 0.00% 25 6 0.00% 26 4 0.00% 27 5 0.00% 28 23 0.00% 29 6 0.00% 30 6 0.00% 31 3 0.00% 32 7 0.00% 33 4 0.00% 34 5 0.00% 35 6 0.00% 36 7 0.00% 37 5 0.00% 38 11 0.00% 39 14 0.00% 40 15 0.00% 41 13 0.00% 42 20 0.00% 43 30 0.00% 44 34 0.00% 45 41 0.00% 46 45 0.00% 47 53 0.00% 48 59 0.00% 49 59 0.00% 50 97 0.00% 51 87 0.00% 52 103 0.00% 53 122 0.00% 54 152 0.00% 55 137 0.00% 56 193 0.00% 57 197 0.00% 58 281 0.00% 59 256 0.00% 60 308 0.00% 61 370 0.00% 62 490 0.00% 63 601 0.00% 64 600 0.00% 65 762 0.00% 66 768 0.00% 67 862 0.00% 68 1026 0.01% 69 1214 0.01% 70 1489 0.01% 71 1748 0.01% 72 2128 0.01% 73 2449 0.01% 74 2739 0.01% 75 3042 0.02% 76 3396 0.02% 77 3598 0.02% 78 4076 0.02% 79 4623 0.02% 80 5402 0.03% 81 6431 0.03% 82 7678 0.04% 83 8766 0.04% 84 9736 0.05% 85 10062 0.05% 86 11019 0.05% 87 11748 0.06% 88 12736 0.06% 89 14013 0.07% 90 15527 0.08% 91 17095 0.08% 92 20249 0.10% 93 21770 0.11% 94 23959 0.12% 95 25227 0.13% 96 26544 0.13% 97 26492 0.13% 98 27635 0.14% 99 29219 0.14% 100 30818 0.15% 101 33542 0.17% 102 36283 0.18% 103 39343 0.20% 104 41668 0.21% 105 44616 0.22% 106 44867 0.22% 107 44927 0.22% 108 46291 0.23% 109 45720 0.23% 110 47360 0.24% 111 50161 0.25% 112 53776 0.27% 113 62416 0.31% 114 60238 0.30% 115 63773 0.32% 116 64616 0.32% 117 62483 0.31% 118 63303 0.31% 119 62406 0.31% 120 63607 0.32% 121 67316 0.33% 122 69553 0.35% 123 73852 0.37% 124 76118 0.38% 125 80040 0.40% 126 81338 0.40% 127 78562 0.39% 128 77801 0.39% 129 80059 0.40% 130 79994 0.40% 131 80613 0.40% 132 82489 0.41% 133 87237 0.43% 134 89723 0.45% 135 90796 0.45% 136 95010 0.47% 137 90616 0.45% 138 90777 0.45% 139 91660 0.45% 140 90502 0.45% 141 91088 0.45% 142 95369 0.47% 143 92335 0.46% 144 97848 0.49% 145 99824 0.50% 146 104701 0.52% 147 102657 0.51% 148 101919 0.51% 149 100548 0.50% 150 100228 0.50% 151 16080512 79.79% 20153024 reads passed initial QC criterion=sequence-density sequence-density=0.61 sequence-density-rank=1 fanout-score=2.09 fanout-score-rank=29 prefix-density=0.64 prefix-fanout=2.0 sequence=CAGGTGCAGTTTGATCC criterion=fanout-score sequence-density=0.01 sequence-density-rank=28 fanout-score=53.64 fanout-score-rank=1 prefix-density=0.16 prefix-fanout=4.5 sequence=ATCACACACATCAAGGCATACATTAACTTCACACAAAGCCCTCGTAAATCATATCACACAGCAAGGCAGGCTACTGAAACCTATAAATAAGAAGCCCCCACCTTTAAGTTTCAGTAGACCCCACGTACACAAGTTTATTGATAAATTAAAATACATTAATACAGGAGAACAGGCGTTTGTCCCCACCACACCGCTTCTTATTATTCACTCATGCCAGTAGTCATTTTAAATTTAAGACAGCAGCAGCA criterion=sequence-density sequence-density=0.53 sequence-density-rank=1 fanout-score=2.09 fanout-score-rank=25 prefix-density=0.53 prefix-fanout=2.1 sequence=ATGTACCCTGACTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=29 fanout-score=37.82 fanout-score-rank=1 prefix-density=0.20 prefix-fanout=1.0 sequence=CCGGCGCACGCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTGGAAAAGTCACACTAGAGCGACACCAACATCGTTACGCTTACACACCGGACGCTTGGATCAGTGGGAAGTGCTCACGCGCGGAGCCCACTGGGCGAACAGCAACGTTATAACGGCCACTCAGTGGTTCGTCACGCGCAGCCCCGGGTTCGTCCCCTATAAGGGCCTAGTACCTTTCGAGCCCCGCGCGTACTAGGCAGATAAGAACCCTCCAGCTCGGGGCCTCAAACCGATATTCCATGTGGGCCAACTGCCATGTTGTGTCCAGTCGCTATCGGAGTAGCCGCGCTGGTGCCACACGACTACAACCCTCGTAATAGGGCTGCGTGCGTCCTAAATACACTCGCTGTTGAGATACTAAAATTATCTGTGGATTGCCGGCATTGAGCCCACGGTAAACCCCAAATACATAAGTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATG SRR7166130 testing PE reads STAR mapping to Ensembl genome Started job on | Apr 15 04:27:27 Started mapping on | Apr 15 04:27:27 Finished on | Apr 15 04:42:23 Mapping speed, Million of reads per hour | 80.97 Number of input reads | 20153024 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 13404148 Uniquely mapped reads % | 66.51% Average mapped length | 288.85 Number of splices: Total | 8436186 Number of splices: Annotated (sjdb) | 8209138 Number of splices: GT/AG | 8285390 Number of splices: GC/AG | 103886 Number of splices: AT/AC | 8081 Number of splices: Non-canonical | 38829 Mismatch rate per base, % | 0.46% Deletion rate per base | 0.06% Deletion average length | 2.43 Insertion rate per base | 0.03% Insertion average length | 2.12 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 407143 % of reads mapped to multiple loci | 2.02% Number of reads mapped to too many loci | 119071 % of reads mapped to too many loci | 0.59% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 30.66% % of reads unmapped: other | 0.21% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 6341733 6341733 6341733 N_multimapping 407143 407143 407143 N_noFeature 490552 13209307 566608 N_ambiguous 197049 1883 77100 UnstrandedReadsAssigned:12716547 PositiveStrandReadsAssigned:192958 NegativeStrandReadsAssigned:12760440 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR7166130 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR7166130-trimmed-pair1.fastq SRR7166130-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,153,024 reads, 12,874,025 reads pseudoaligned [quant] estimated average fragment length: 202.963 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,176 rounds 52401 SRR7166130.ke.tsv 34699 SRR7166130.se.tsv 87100 total ==> SRR7166130.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1816.04 1019 32.6497 Potri.005G024800.1.v4.1 1035 833.037 314 21.9328 Potri.004G059700.1.v4.1 961 759.037 34 2.60643 Potri.007G009000.2.v4.1 1416 1214.04 0 0 Potri.003G141000.2.v4.1 2943 2741.04 270 5.73163 Potri.016G087400.1.v4.1 270 97.7804 635.357 378.09 Potri.015G069301.1.v4.1 564 363.485 0 0 Potri.010G195200.1.v4.1 1773 1571.04 352 13.0372 Potri.012G127500.1.v4.1 977 775.037 10279 771.716 ==> SRR7166130.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 39 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 1100 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 1 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 548 SRR7166130 completed mapping pipeline successfully