Starting /dee2/code/volunteer_pipeline.sh SRR7170015 current disk space = 2796559810560 free memory = 1557207348 SRR7170015_1.fastq is conventional basespace SRR7170015_1.fastq read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7170015_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14057190 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0 30.0 30.0 30.0 30.0 30.0 2 30.0 30.0 30.0 30.0 30.0 30.0 3 30.0 30.0 30.0 30.0 30.0 30.0 4 30.0 30.0 30.0 30.0 30.0 30.0 5 30.0 30.0 30.0 30.0 30.0 30.0 6 30.0 30.0 30.0 30.0 30.0 30.0 7 30.0 30.0 30.0 30.0 30.0 30.0 8 30.0 30.0 30.0 30.0 30.0 30.0 9 30.0 30.0 30.0 30.0 30.0 30.0 10-14 30.0 30.0 30.0 30.0 30.0 30.0 15-19 30.0 30.0 30.0 30.0 30.0 30.0 20-24 30.0 30.0 30.0 30.0 30.0 30.0 25-29 30.0 30.0 30.0 30.0 30.0 30.0 30-34 30.0 30.0 30.0 30.0 30.0 30.0 35-39 30.0 30.0 30.0 30.0 30.0 30.0 40-44 30.0 30.0 30.0 30.0 30.0 30.0 45-49 30.0 30.0 30.0 30.0 30.0 30.0 50-54 30.0 30.0 30.0 30.0 30.0 30.0 55-59 30.0 30.0 30.0 30.0 30.0 30.0 60-64 30.0 30.0 30.0 30.0 30.0 30.0 65-69 30.0 30.0 30.0 30.0 30.0 30.0 70-74 30.0 30.0 30.0 30.0 30.0 30.0 75-79 30.0 30.0 30.0 30.0 30.0 30.0 80-84 30.0 30.0 30.0 30.0 30.0 30.0 85-89 30.0 30.0 30.0 30.0 30.0 30.0 90-94 30.0 30.0 30.0 30.0 30.0 30.0 95-99 30.0 30.0 30.0 30.0 30.0 30.0 100-104 30.0 30.0 30.0 30.0 30.0 30.0 105-109 30.0 30.0 30.0 30.0 30.0 30.0 110-114 30.0 30.0 30.0 30.0 30.0 30.0 115-119 30.0 30.0 30.0 30.0 30.0 30.0 120-124 30.0 30.0 30.0 30.0 30.0 30.0 125-129 30.0 30.0 30.0 30.0 30.0 30.0 130-134 30.0 30.0 30.0 30.0 30.0 30.0 135-139 30.0 30.0 30.0 30.0 30.0 30.0 140-144 30.0 30.0 30.0 30.0 30.0 30.0 145-149 30.0 30.0 30.0 30.0 30.0 30.0 150-151 30.0 30.0 30.0 30.0 30.0 30.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 1.405719E7 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.126331451467806 14.139782907292625 9.725651493407007 33.00823414783257 2 23.559936231921174 16.143468218043576 31.431267557740917 28.865327992294336 3 19.708106669967467 21.43816082730617 26.20641821018283 32.647314292543534 4 21.862655338655877 27.563517317472414 23.612535649016625 26.96129169485509 5 22.251018873615568 30.65767055862516 24.37928917514809 22.712021392611184 6 20.966978464401492 33.27285182885058 24.904443918023446 20.855725788724488 7 15.372538892908183 27.45942823565734 38.61954629623702 18.54848657519746 8 18.54789613002314 26.75756676832283 29.189951903616585 25.504585198037443 9 17.431222029438313 25.709860932376955 32.49627414867409 24.362642889510635 10-14 20.050650972933077 29.324432596676324 27.005429467192755 23.619486963197843 15-19 19.9544076732263 28.608760356799618 27.485404977808507 23.951426992165576 20-24 20.066866848922153 28.708581160246112 27.413191398849985 23.811360591981753 25-29 20.07897176502592 28.819984895970567 27.228460396312887 23.872582942690624 30-34 20.080256438164383 28.72711687044139 27.288081046069664 23.904545645324564 35-39 20.147204384375538 28.585535231436726 27.166272917987165 24.10098746620057 40-44 20.253084647701527 28.635830673096812 27.26384478874188 23.847239890459782 45-49 20.38374168280182 28.389558064198024 27.2183341360895 24.008366116910654 50-54 20.305021131534822 28.48183883123156 27.223016833378505 23.99012320385511 55-59 20.40911163610935 28.3945112785699 27.122330992182647 24.0740460931381 60-64 20.30266504187537 28.456344404536043 27.21884103437458 24.02214951921401 65-69 20.39649460525183 28.286411437847818 27.23676353524424 24.080330421656107 70-74 20.44260339370813 28.420499402796718 27.21340467049247 23.923492533002687 75-79 20.522010444477168 28.16280209629378 27.19304355991489 24.122143899314157 80-84 20.511649032672814 28.274442093852308 27.134913782628978 24.0789950908459 85-89 20.677212361116585 28.106193498640565 27.19160320311131 24.024990937131545 90-94 20.68256347036293 28.172227644449293 27.077413789482605 24.06779509570517 95-99 20.700678957286975 28.017279501654823 27.19545183532918 24.08658970572902 100-104 20.885699335534063 28.20381661958612 26.986915974387486 23.92356807049233 105-109 20.87714555419208 28.02450709486406 27.019031965427644 24.07931538551622 110-114 20.910612710281125 28.135201439393153 26.892688929990637 24.06149692033508 115-119 20.98124797558215 28.20279016319095 26.790426231885384 24.025535629341515 120-124 21.06640313986467 28.13735170730689 26.62762660396172 24.168618548866718 125-129 21.09024307466512 28.050639248657355 26.655887484701324 24.203230191976203 130-134 21.184121761136648 28.103773101023204 26.50498662613995 24.207118511700198 135-139 21.144973326355814 28.143147561155025 26.43947187123575 24.272407241253415 140-144 21.201518483719916 28.11790216908603 26.274740482144704 24.40583886504935 145-149 21.184841595227578 28.285205605955582 26.146885342390092 24.38306745642675 150-151 21.0535071376285 28.280876192183502 26.055072173030315 24.610544497157683 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 603.0 1 497.5 2 377.5 3 361.0 4 366.0 5 346.5 6 322.0 7 323.0 8 321.5 9 313.0 10 293.0 11 304.5 12 352.0 13 382.5 14 430.0 15 500.0 16 608.0 17 782.0 18 1037.0 19 1352.0 20 1696.5 21 2191.0 22 2969.5 23 4060.0 24 5711.5 25 8256.5 26 12378.0 27 18797.5 28 26466.0 29 36725.0 30 51638.5 31 72429.5 32 96822.0 33 126580.5 34 162565.0 35 208517.0 36 268962.0 37 339305.0 38 416250.0 39 506649.0 40 610532.0 41 711135.5 42 804214.5 43 883484.5 44 936613.5 45 956913.5 46 952075.5 47 925914.5 48 864532.0 49 772964.5 50 664200.5 51 556420.5 52 464737.0 53 386612.0 54 305559.0 55 226274.0 56 165432.0 57 125270.0 58 95923.5 59 69974.5 60 50360.5 61 35791.0 62 26555.0 63 20049.0 64 15987.0 65 13439.5 66 10141.0 67 7761.0 68 7024.0 69 5998.5 70 4128.5 71 1554.5 72 504.0 73 256.5 74 142.5 75 66.0 76 37.0 77 23.5 78 14.5 79 10.0 80 6.5 81 3.5 82 2.5 83 0.5 84 0.5 85 2.0 86 2.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2081923912247042 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 3.300801938367483E-4 15-19 0.0 20-24 0.0 25-29 9.959316193350165E-5 30-34 0.0 35-39 0.0 40-44 7.583307901508054E-4 45-49 0.001441255329123388 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 1.621945780059884E-4 85-89 0.002718893320784595 90-94 0.004589822005678233 95-99 0.005779248911055481 100-104 0.0015408484910568899 105-109 9.54671595105423E-4 110-114 3.115843209062409E-4 115-119 4.4390095033217873E-4 120-124 5.306892771599444E-4 125-129 6.658514254982681E-4 130-134 0.0020701150087606415 135-139 0.004396326719636002 140-144 0.003064623868639465 145-149 0.0021099522735340418 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 1.405719E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.57980047671165 #Duplication Level Percentage of deduplicated Percentage of total 1 64.97121964797081 26.365191300432635 2 16.802735795215323 13.637033320654773 3 6.426710491050914 7.823838883453067 4 3.200174843606398 5.194498265765581 5 2.0070722864102133 4.072329646243196 6 1.2934375036168388 3.1492461495520394 7 0.896360352620848 2.5461886985212234 8 0.688215260085075 2.234211035142445 9 0.5090386715568821 1.8591018954037766 >10 2.932621170332296 21.601944375961523 >50 0.18936777583156736 5.207807065909596 >100 0.0807066756798128 5.552787710797403 >500 0.0020410136879472347 0.5244512204926087 >1k 2.8095506690978766E-4 0.15541395876118738 >5k 0.0 0.0 >10k+ 1.755726802666801E-5 0.07595647290886108 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0013800766725070943 2.8455189123857614E-5 0.0 7.113797280964404E-6 0.0 2 0.001394304267069023 1.3516214833832367E-4 0.0 1.4227594561928807E-5 0.0 3 0.0014014180643499873 1.4938974290025248E-4 0.0 1.4227594561928807E-5 0.0 4 0.0014512146453167383 1.5650354018121687E-4 0.0 2.8455189123857614E-5 0.0 5 0.0014938974290025246 1.6361733746218126E-4 0.0 2.8455189123857614E-5 0.0 6 0.0015650354018121688 1.6361733746218126E-4 0.0 2.8455189123857614E-5 0.0 7 0.0016006043882169907 1.7073113474314568E-4 0.0 2.8455189123857614E-5 0.0 8 0.0016290595773408483 1.7073113474314568E-4 0.0 4.9796580966750824E-5 0.0 9 0.001657514766464706 1.7073113474314568E-4 0.0 6.402417552867963E-5 0.0 10-11 0.0017215389419933855 1.7784493202411007E-4 0.0 6.758107416916182E-5 0.0 12-13 0.0017571079283982076 1.7784493202411007E-4 0.0 1.1026385785494825E-4 0.0 14-15 0.0018033476107244763 1.7784493202411007E-4 0.0 1.3871904697880587E-4 0.0 16-17 0.0018389165971292982 1.9207252658603888E-4 0.0 1.4227594561928806E-4 0.0 18-19 0.0018851562794555668 1.9207252658603888E-4 0.0 1.4227594561928806E-4 0.0 20-21 0.001959851150905693 1.9918632386700327E-4 0.0 1.4938974290025248E-4 0.0 22-23 0.002045216718277266 2.063001211479677E-4 0.0 1.5650354018121687E-4 0.0 24-25 0.0021199115897273923 2.0985701978844988E-4 0.0 1.5650354018121687E-4 0.0 26-27 0.0021839357652560717 2.169708170694143E-4 0.0 1.6006043882169907E-4 0.0 28-29 0.00229419962311102 2.2764151299086092E-4 0.0 1.6361733746218126E-4 0.0 30-31 0.002436475568730308 2.2764151299086092E-4 0.0 1.7784493202411007E-4 0.0 32-33 0.00264988948715924 2.311984116313431E-4 0.0 1.9207252658603888E-4 0.0 34-35 0.002966453466162156 2.383122089123075E-4 0.0 2.169708170694143E-4 0.0 36-37 0.0032367777628388036 2.4186910755278972E-4 0.0 2.205277157098965E-4 0.0 38-39 0.0035391141472797907 2.489829048337541E-4 0.0 2.205277157098965E-4 0.0 40-41 0.003976612680059101 2.560967021147185E-4 0.0 2.347553102718253E-4 0.0 42-43 0.004520818172052878 2.667673980361651E-4 0.0 2.4186910755278972E-4 0.0 44-45 0.005193072015104014 3.0589328308146935E-4 0.0 2.703242966766473E-4 0.0 46-47 0.005947134526886241 3.2012087764339813E-4 0.0 2.9877948580050496E-4 0.0 48-49 0.0069928627271880085 3.556898640482202E-4 0.0 2.9877948580050496E-4 0.0 50-51 0.008237777251356779 3.69917458610149E-4 0.0 3.0233638444098713E-4 0.0 52-53 0.010115819733531381 3.912588504530422E-4 0.0 3.165639790029159E-4 0.0 54-55 0.012374450370237579 4.23270938217382E-4 0.0 3.3079157356484475E-4 0.0 56-57 0.015102591627487429 4.41055431419793E-4 0.0 3.4146226948629136E-4 0.0 58-59 0.01851365742370986 4.4816922870075744E-4 0.0 3.52132965407738E-4 0.0 60-61 0.023208763629146367 4.979658096675082E-4 0.0 3.663605599696668E-4 0.0 62-63 0.029376425871742502 5.228641001508836E-4 0.0 3.912588504530422E-4 0.0 64-65 0.03794143779802364 5.335347960723302E-4 0.0 4.1971403957689977E-4 0.0 66-67 0.048174635186690934 5.406485933532947E-4 0.0 4.7306751918413284E-4 0.0 68-69 0.0600333352540586 5.477623906342591E-4 0.0 4.8373821510557945E-4 0.0 70-71 0.07556631161704437 5.548761879152235E-4 0.0 4.908520123865438E-4 0.0 72-73 0.09721359674301905 5.691037824771524E-4 0.0 5.050796069484727E-4 0.0 74-75 0.12545181504980726 5.868882756795633E-4 0.0 5.050796069484727E-4 0.0 76-77 0.1592174538439048 6.153434648034209E-4 0.0 5.050796069484727E-4 0.0 78-79 0.19921122215748666 6.473555525677607E-4 0.0 5.193072015104015E-4 0.0 80-81 0.24689856223043155 6.900383362535472E-4 0.0 5.477623906342591E-4 0.0 82-83 0.3082194947923447 7.184935253774047E-4 0.0 5.762175797581167E-4 0.0 84-85 0.3863645579237387 7.540625117822267E-4 0.0 5.940020729605278E-4 0.0 86-87 0.48058680291011213 7.611763090631912E-4 0.0 6.117865661629386E-4 0.0 88-89 0.5838364566460296 7.682901063441555E-4 0.0 6.18900363443903E-4 0.0 90-91 0.7008441943233321 7.931883968275309E-4 0.0 6.366848566463141E-4 0.0 92-93 0.8408472817113519 8.038590927489776E-4 0.0 6.758107416916183E-4 0.0 94-95 1.0088680596904502 8.145297886704241E-4 0.0 6.829245389725827E-4 0.0 96-97 1.1951108294047388 8.358711805133174E-4 0.0 6.829245389725827E-4 0.0 98-99 1.3940197151777844 8.46541876434764E-4 0.0 6.829245389725827E-4 0.0 100-101 1.6066155469194057 8.536556737157284E-4 0.0 6.86481437613065E-4 0.0 102-103 1.84615844276132 8.678832682776572E-4 0.0 6.971521335345115E-4 0.0 104-105 2.121305893994461 9.105660519634436E-4 0.0 6.971521335345115E-4 0.0 106-107 2.4225467536541796 9.5324883564923E-4 0.0 7.113797280964404E-4 0.0 108-109 2.734458309235345 9.674764302111589E-4 0.0 7.184935253774047E-4 0.0 110-111 3.051157450386599 9.674764302111589E-4 0.0 7.220504240178869E-4 0.0 112-113 3.3938255085120144 9.781471261326054E-4 0.0 7.327211199393335E-4 0.0 114-115 3.7767434316531254 9.817040247730876E-4 0.0 7.362780185798158E-4 0.0 116-117 4.189606884448457 9.994885179754986E-4 0.0 7.505056131417445E-4 0.0 118-119 4.610782809366595 0.001042171301661285 0.0 7.540625117822267E-4 0.0 120-121 5.033402835132769 0.0010706264907851426 0.0 7.825177009060844E-4 0.0 122-123 5.476453686689872 0.0010741833894256248 0.0 8.074159913894598E-4 0.0 124-125 5.962290471993336 0.0010919678826280359 0.0 8.216435859513886E-4 0.0 126-127 6.486129162371712 0.0011310937676733402 0.0 8.749970655586217E-4 0.0 128-129 7.020140582861867 0.0011417644635947867 0.0 8.749970655586217E-4 0.0 130-131 7.548396941351721 0.0011559920581567156 0.0 8.749970655586217E-4 0.0 132-133 8.091812801847311 0.0011631058554376799 0.0 8.785539641991038E-4 0.0 134-135 8.676406877903762 0.0011737765513591266 0.0 9.034522546824792E-4 0.0 136-137 9.304053655104612 0.0012057886391234663 0.0 9.212367478848903E-4 0.0 138-139 9.943107406245487 0.0012200162336853952 0.0 9.35464342446819E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTCG 2285 0.0 13.642837 3 GCGGGAT 2820 0.0 12.621532 1 GTCCCGT 1920 0.0 12.106367 1 GTCCGGA 2165 0.0 11.742898 1 GTCGGTT 3990 0.0 11.287141 1 GCCTTAT 9110 0.0 10.764169 1 GGCCCGG 1510 1.8189894E-12 10.583051 1 GTCCTAT 3675 0.0 10.475716 1 TTCGCAC 3610 0.0 10.443061 8 GCCCGAC 1325 1.9826984E-10 10.416045 1 CGGGATT 3650 0.0 10.328397 2 GGCGTAT 1585 5.456968E-12 10.082274 1 GGCCTAT 3410 0.0 10.011718 1 GGCCCGT 1540 2.910383E-11 9.9052105 1 GTTCCGA 4060 0.0 9.66124 1 TATGCCG 24955 0.0 9.645495 145 GTCGTAT 2135 0.0 9.526322 1 GTCCGGG 1755 3.6379788E-12 9.519537 1 GCCCTAT 3075 0.0 9.448873 1 CCCGTCT 3265 0.0 9.343966 1 >>END_MODULE SRR7170015 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7170015_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14057190 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0 30.0 30.0 30.0 30.0 30.0 2 30.0 30.0 30.0 30.0 30.0 30.0 3 30.0 30.0 30.0 30.0 30.0 30.0 4 30.0 30.0 30.0 30.0 30.0 30.0 5 30.0 30.0 30.0 30.0 30.0 30.0 6 30.0 30.0 30.0 30.0 30.0 30.0 7 30.0 30.0 30.0 30.0 30.0 30.0 8 30.0 30.0 30.0 30.0 30.0 30.0 9 30.0 30.0 30.0 30.0 30.0 30.0 10-14 30.0 30.0 30.0 30.0 30.0 30.0 15-19 30.0 30.0 30.0 30.0 30.0 30.0 20-24 30.0 30.0 30.0 30.0 30.0 30.0 25-29 30.0 30.0 30.0 30.0 30.0 30.0 30-34 30.0 30.0 30.0 30.0 30.0 30.0 35-39 30.0 30.0 30.0 30.0 30.0 30.0 40-44 30.0 30.0 30.0 30.0 30.0 30.0 45-49 30.0 30.0 30.0 30.0 30.0 30.0 50-54 30.0 30.0 30.0 30.0 30.0 30.0 55-59 30.0 30.0 30.0 30.0 30.0 30.0 60-64 30.0 30.0 30.0 30.0 30.0 30.0 65-69 30.0 30.0 30.0 30.0 30.0 30.0 70-74 30.0 30.0 30.0 30.0 30.0 30.0 75-79 30.0 30.0 30.0 30.0 30.0 30.0 80-84 30.0 30.0 30.0 30.0 30.0 30.0 85-89 30.0 30.0 30.0 30.0 30.0 30.0 90-94 30.0 30.0 30.0 30.0 30.0 30.0 95-99 30.0 30.0 30.0 30.0 30.0 30.0 100-104 30.0 30.0 30.0 30.0 30.0 30.0 105-109 30.0 30.0 30.0 30.0 30.0 30.0 110-114 30.0 30.0 30.0 30.0 30.0 30.0 115-119 30.0 30.0 30.0 30.0 30.0 30.0 120-124 30.0 30.0 30.0 30.0 30.0 30.0 125-129 30.0 30.0 30.0 30.0 30.0 30.0 130-134 30.0 30.0 30.0 30.0 30.0 30.0 135-139 30.0 30.0 30.0 30.0 30.0 30.0 140-144 30.0 30.0 30.0 30.0 30.0 30.0 145-149 30.0 30.0 30.0 30.0 30.0 30.0 150-151 30.0 30.0 30.0 30.0 30.0 30.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 1.405719E7 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.847770344497064 23.08431316858853 14.07317288335969 24.99474360355471 2 27.454546826016607 28.392729484677346 26.83006425717558 17.322659432130465 3 20.519564205066253 30.330309363843334 30.19981798819521 18.950308442895203 4 23.0270927071566 34.13461148327607 23.660565629041823 19.177730180525508 5 24.179224433819318 34.853921522216005 22.465058517883897 18.501795526080777 6 22.626571421168634 35.79836438093985 23.25420030311598 18.320863894775535 7 21.13009349575585 23.454714125467575 35.62558363222445 19.789608746552126 8 23.264049410037845 26.27669253914664 25.783059088177474 24.676198962638043 9 21.837755128404694 25.981454446766893 29.052550574074274 23.12823985075414 10-14 23.88758329766968 28.3787239216769 26.00280336905982 21.730889411593598 15-19 23.775154475484154 27.808370694802736 27.128105908407314 21.2883689213058 20-24 23.661301495463544 28.037389870184676 27.059981196922365 21.241327437429412 25-29 23.861705283276297 28.00069686291114 26.97174785480692 21.16584999900564 30-34 23.719220032457127 27.868438196319005 27.263110709698623 21.149231061525242 35-39 23.72695023844437 27.7973117404471 27.19867201039007 21.277066010718453 40-44 23.902611699034924 27.799783660589654 27.221257342510125 21.076347297865293 45-49 23.83633889421872 27.628135766515765 27.34059252888752 21.194932810377992 50-54 23.758844272933647 27.85021759134715 27.327230471640508 21.063707664078702 55-59 24.020813034482234 27.583553156831908 27.40124485114886 20.994388957536994 60-64 23.763495394613663 27.789238032806 27.5375236829242 20.909742889656137 65-69 23.960517338881456 27.620118006677245 27.48100123814835 20.93836341629295 70-74 23.977198501961368 27.680933099329323 27.417963276918506 20.923905121790796 75-79 23.907479833153438 27.48870792814393 27.648723754360503 20.955088484342124 80-84 23.941344494357033 27.69766275732079 27.483120692196966 20.87787205612521 85-89 24.208514036379565 27.551775625018315 27.450032580157995 20.78967775844412 90-94 24.121298753589127 27.634098674992885 27.518069822631137 20.726532748786855 95-99 24.17800826158233 27.614573909902667 27.464342356427686 20.74307547208732 100-104 24.437859183156153 27.643259125250253 27.287463410050417 20.63141828154318 105-109 24.44618400585368 27.559017443467216 27.3775868322199 20.6172117184592 110-114 24.564161136018207 27.720606254720586 27.138713313010875 20.57651929625033 115-119 24.844890992141224 27.70331881442028 27.073989091705442 20.377801101733052 120-124 24.897490829198087 27.686241866345636 27.04645813248863 20.369809171967642 125-129 25.10072598509947 27.807002310938216 26.8796015470001 20.21267015696221 130-134 25.42810418105575 27.70610431626791 26.724237816938402 20.141553685737932 135-139 25.437197881968576 27.70875554581609 26.804212282287416 20.04983428992792 140-144 25.604476550391407 27.824852500121867 26.603912406295493 19.966758543191233 145-149 25.842538250687124 27.932054620566376 26.485641234835327 19.73976589391117 150-151 25.990938679325538 27.801768340037157 26.504017702336856 19.70327527830045 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5378.0 1 3080.5 2 544.5 3 266.0 4 214.0 5 200.0 6 199.5 7 194.5 8 200.0 9 217.0 10 228.5 11 227.5 12 241.5 13 291.5 14 337.0 15 378.0 16 434.0 17 527.0 18 693.5 19 805.0 20 892.0 21 1159.5 22 1491.5 23 1931.0 24 2535.5 25 3305.5 26 4599.0 27 6466.5 28 9140.0 29 13246.5 30 20031.5 31 30158.0 32 45092.0 33 69690.0 34 104029.0 35 150315.0 36 211434.5 37 292948.5 38 398273.0 39 520251.5 40 650201.0 41 768537.0 42 877065.5 43 967641.5 44 1016620.0 45 1032446.5 46 1023426.5 47 980147.0 48 900990.0 49 793750.5 50 673278.5 51 559843.5 52 461848.5 53 372249.0 54 284252.0 55 208867.5 56 153867.5 57 115413.5 58 86520.5 59 63641.5 60 44734.0 61 31047.0 62 23187.0 63 17876.5 64 13326.0 65 10019.0 66 7595.5 67 5605.5 68 4509.5 69 3608.0 70 2545.5 71 1457.0 72 798.0 73 420.5 74 263.5 75 166.0 76 104.0 77 69.5 78 54.5 79 38.5 80 28.0 81 21.0 82 18.5 83 16.5 84 14.5 85 11.0 86 10.0 87 11.0 88 8.5 89 7.5 90 7.0 91 5.5 92 7.0 93 6.0 94 3.5 95 4.5 96 4.0 97 2.5 98 2.0 99 2.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0881897448921157 2 0.0326950123033124 3 0.04590533385406329 4 0.062316864181248176 5 0.06953736842142705 6 0.06005467664590149 7 0.059165451985780944 8 0.056191884722337826 9 0.05230775140693125 10-14 0.06265690369127827 15-19 0.07373877709556462 20-24 0.06448088131411754 25-29 0.0564963552459631 30-34 0.057860781564452075 35-39 0.06407966314747116 40-44 0.07247963497683392 45-49 0.07149650819260464 50-54 0.05861626683569049 55-59 0.060969510976233515 60-64 0.05868313653013156 65-69 0.05680367128850076 70-74 0.04698236276240131 75-79 0.04680736334928958 80-84 0.056321355832851375 85-89 0.07979973237894629 90-94 0.09071656568631427 95-99 0.05840854395508633 100-104 0.05334352029103968 105-109 0.05820935763121933 110-114 0.06136646086451132 115-119 0.04823865936221962 120-124 0.042046810208868204 125-129 0.06987029413417617 130-134 0.1211678863272105 135-139 0.15789215341046112 140-144 0.1800786643703329 145-149 0.11394453656811923 150-151 0.07278125998154682 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 1.405719E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.87849469750706 #Duplication Level Percentage of deduplicated Percentage of total 1 64.1604017030809 25.586202391062347 2 16.6667252175553 13.292878264261725 3 6.686597774981968 7.9995436174194285 4 3.472740712526396 5.539506883612031 5 2.0796569621893335 4.146679456965048 6 1.3900310047222222 3.3259406430711334 7 0.947657377881006 2.6453804803216805 8 0.7166639525984996 2.2863583706874975 9 0.5545086377490803 1.9901672793178709 >10 3.053431188928969 21.9714827363489 >50 0.19078136824685693 5.199019861540315 >100 0.07843162689580635 5.1252314718277185 >500 0.0018903382434465365 0.5135560050409016 >1k 4.64279436868449E-4 0.30212176004911584 >5k 0.0 0.0 >10k+ 1.785496320414532E-5 0.07593077847432829 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0013445076861022722 0.0 0.0 7.113797280964404E-6 0.0 2 0.0013658490779451654 7.113797280964404E-6 0.0 2.134139184289321E-5 0.0 3 0.0013871904697880587 7.113797280964404E-6 0.0 2.134139184289321E-5 0.0 4 0.0013871904697880587 1.4227594561928807E-5 0.0 4.268278368578642E-5 0.0 5 0.001408531861630952 1.4227594561928807E-5 0.0 5.691037824771523E-5 0.0 6 0.0014369870507548095 1.4227594561928807E-5 0.0 8.536556737157284E-5 0.0 7 0.0014725560371596316 1.4227594561928807E-5 0.0 8.536556737157284E-5 0.0 8 0.0015010112262834891 1.4227594561928807E-5 0.0 1.2093455377639486E-4 0.0 9 0.0015152388208454178 1.4227594561928807E-5 0.0 1.2804835105735925E-4 0.0 10-11 0.0015721491990931332 1.7784493202411008E-5 0.0 1.3516214833832367E-4 0.0 12-13 0.0016006043882169907 2.134139184289321E-5 0.0 1.4227594561928806E-4 0.0 14-15 0.0016326164759813307 2.134139184289321E-5 0.0 1.4227594561928806E-4 0.0 16-17 0.0016504009691837417 2.8455189123857614E-5 0.0 1.6361733746218126E-4 0.0 18-19 0.001678856158307599 3.5568986404822016E-5 0.0 1.6361733746218126E-4 0.0 20-21 0.0017357665365553144 3.5568986404822016E-5 0.0 1.7428803338362788E-4 0.0 22-23 0.0018246890025673695 3.912588504530422E-5 0.0 1.991863238670033E-4 0.0 24-25 0.001902940772657978 4.623968232626862E-5 0.0 2.383122089123075E-4 0.0 26-27 0.001945623556343764 6.046727688819743E-5 0.0 2.4186910755278972E-4 0.0 28-29 0.0020381029209963018 6.758107416916182E-5 0.0 2.4186910755278972E-4 0.0 30-31 0.0021625943734131783 8.536556737157284E-5 0.0 2.632104993956829E-4 0.0 32-33 0.0023368824067968065 8.536556737157284E-5 0.0 2.845518912385761E-4 0.0 34-35 0.002632104993956829 8.536556737157284E-5 0.0 2.845518912385761E-4 0.0 36-37 0.0028882016960715476 9.959316193350165E-5 0.0 2.916656885195405E-4 0.0 38-39 0.0032012087764339814 9.959316193350165E-5 0.0 3.094501817219516E-4 0.0 40-41 0.003642264207853774 1.0315006057398386E-4 0.0 3.1300708036243374E-4 0.0 42-43 0.004147343814802247 1.0670695921446605E-4 0.0 3.2012087764339813E-4 0.0 44-45 0.004784028671448561 1.0670695921446605E-4 0.0 3.3434847220532697E-4 0.0 46-47 0.0055131928927474125 1.1382075649543046E-4 0.0 3.4857606676725575E-4 0.0 48-49 0.006583819383532556 1.1382075649543046E-4 7.113797280964404E-6 3.521329654077379E-4 0.0 50-51 0.007835847704982291 1.1382075649543046E-4 7.113797280964404E-6 3.5568986404822014E-4 0.0 52-53 0.009731674680359303 1.1382075649543046E-4 7.113797280964404E-6 3.805881545315956E-4 0.0 54-55 0.01195473633066068 1.2093455377639486E-4 7.113797280964404E-6 4.4816922870075744E-4 0.0 56-57 0.014622410311022332 1.2093455377639486E-4 7.113797280964404E-6 4.623968232626862E-4 0.0 58-59 0.018040589904525728 1.2093455377639486E-4 7.113797280964404E-6 4.801813164650972E-4 0.0 60-61 0.02274992370452416 1.2804835105735925E-4 7.113797280964404E-6 5.584330865557056E-4 0.0 62-63 0.028956711832165603 1.2804835105735925E-4 7.113797280964404E-6 5.726606811176345E-4 0.0 64-65 0.03754662204893012 1.2804835105735925E-4 7.113797280964404E-6 5.904451743200455E-4 0.0 66-67 0.04782961601856417 1.2804835105735925E-4 7.113797280964404E-6 5.904451743200455E-4 0.0 68-69 0.059702543680493755 1.3516214833832364E-4 7.113797280964404E-6 6.046727688819743E-4 0.0 70-71 0.0753208856108511 1.4227594561928806E-4 7.113797280964404E-6 6.864814376130649E-4 0.0 72-73 0.09716024326341181 1.4938974290025248E-4 7.113797280964404E-6 7.931883968275309E-4 0.0 74-75 0.125700797954641 1.4938974290025248E-4 7.113797280964404E-6 8.252004845918709E-4 0.0 76-77 0.15960159889707687 1.4938974290025248E-4 7.113797280964404E-6 8.323142818728352E-4 0.0 78-79 0.19977321214268284 1.5650354018121687E-4 1.4227594561928807E-5 8.643263696371749E-4 0.0 80-81 0.2480616680858692 1.5650354018121687E-4 1.4227594561928807E-5 8.749970655586217E-4 0.0 82-83 0.3100619682881145 1.6006043882169907E-4 1.4227594561928807E-5 9.17679849244408E-4 0.0 84-85 0.388801033492469 1.6717423610266348E-4 1.4227594561928807E-5 9.319074438063368E-4 0.0 86-87 0.4837666702947033 1.7073113474314568E-4 2.134139184289321E-5 9.35464342446819E-4 0.0 88-89 0.5880122556499556 1.7073113474314568E-4 2.134139184289321E-5 9.461350383682657E-4 0.0 90-91 0.7062044405745387 1.7073113474314568E-4 2.134139184289321E-5 9.568057342897122E-4 0.0 92-93 0.8480962411406547 1.7073113474314568E-4 2.134139184289321E-5 9.603626329301945E-4 0.0 94-95 1.0183151824795709 1.7073113474314568E-4 2.134139184289321E-5 9.674764302111589E-4 0.0 96-97 1.2062972756290553 1.778449320241101E-4 2.134139184289321E-5 9.674764302111589E-4 0.0 98-99 1.4057325824008924 1.849587293050745E-4 2.134139184289321E-5 9.8526092341357E-4 0.0 100-101 1.619121602539341 1.9207252658603888E-4 2.134139184289321E-5 0.0010101592138969454 0.0 102-103 1.8587569777459079 1.9207252658603888E-4 2.134139184289321E-5 0.001024386808458874 0.0 104-105 2.134694060477236 1.9207252658603888E-4 2.134139184289321E-5 0.001038614403020803 0.0 106-107 2.43707668460055 1.9207252658603888E-4 2.134139184289321E-5 0.0010457282003017673 0.0 108-109 2.7510654689877567 1.956294252265211E-4 2.134139184289321E-5 0.0010919678826280359 0.0 110-111 3.0703967151329676 1.991863238670033E-4 2.8455189123857614E-5 0.0011026385785494826 0.0 112-113 3.415938747359892 2.027432225074855E-4 2.8455189123857614E-5 0.001109752375830447 0.0 114-115 3.8021076758584043 2.063001211479677E-4 2.8455189123857614E-5 0.0011168661731114113 0.0 116-117 4.21876633950313 2.063001211479677E-4 2.8455189123857614E-5 0.0011239799703923758 0.0 118-119 4.643630768311448 2.134139184289321E-4 2.8455189123857614E-5 0.001127536869032858 0.0 120-121 5.069533811522787 2.134139184289321E-4 2.8455189123857614E-5 0.0011382075649543045 0.0 122-123 5.515785871856324 2.134139184289321E-4 2.8455189123857614E-5 0.0011453213622352688 0.0 124-125 6.005641952623533 2.134139184289321E-4 2.8455189123857614E-5 0.0011453213622352688 0.0 126-127 6.534371378632572 2.134139184289321E-4 2.8455189123857614E-5 0.0011524351595162334 0.0 128-129 7.071847218398556 2.134139184289321E-4 2.8455189123857614E-5 0.0011524351595162334 0.0 130-131 7.6044643346216425 2.134139184289321E-4 2.8455189123857614E-5 0.001166662754078162 0.0 132-133 8.151444207554995 2.134139184289321E-4 2.8455189123857614E-5 0.0011702196527186444 0.0 134-135 8.740018453190146 2.134139184289321E-4 2.8455189123857614E-5 0.0011737765513591266 0.0 136-137 9.371001601315768 2.134139184289321E-4 2.8455189123857614E-5 0.001180890348640091 0.0 138-139 10.014569056831416 2.134139184289321E-4 2.8455189123857614E-5 0.0012164593350449129 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCACGC 3800 0.0 17.93138 5 CGGTGGG 5135 0.0 15.543323 145 GCGTCGG 3715 0.0 14.06259 145 GCACGCA 5210 0.0 13.912855 6 CTGCACG 4960 0.0 13.591604 4 CACGCAA 5325 0.0 12.796192 7 ACGCAAA 5795 0.0 12.258765 8 TCTCGGG 4460 0.0 11.876258 145 TCGCCGG 5195 0.0 11.872029 145 AGAGCGG 4280 0.0 11.358543 145 TCGTACG 1240 7.9890015E-9 9.937947 4 GGACTGC 8345 0.0 9.7309885 1 GCCGTAT 21520 0.0 9.474486 145 CGTACGG 1235 7.942617E-8 9.39123 5 CGCAAAG 8305 0.0 9.07779 9 GTCGCCG 28535 0.0 8.696406 145 CGTATCA 21525 0.0 8.494719 145 CGCCGTA 24445 0.0 8.400168 145 TTAGGGC 5700 0.0 8.266297 7 TAATTAG 6015 0.0 8.194883 4 >>END_MODULE skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7170015 SRR7170015_1.fastq SRR7170015_2.fastq Input file: SRR7170015_1.fastq Paired file: SRR7170015_2.fastq trimmed: SRR7170015-trimmed-pair1.fastq, SRR7170015-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Apr 15 04:21:46 2025 >> started Tue Apr 15 04:22:11 2025 >> done (24.365s) 14057190 read pairs processed; of these: 64 ( 0.00%) short read pairs filtered out after trimming by size control 11636 ( 0.08%) empty read pairs filtered out after trimming by size control 14045490 (99.92%) read pairs available; of these: 2102282 (14.97%) trimmed read pairs available after processing 11943208 (85.03%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 5 0.00% 20 2 0.00% 21 8 0.00% 22 6 0.00% 23 6 0.00% 24 3 0.00% 25 2 0.00% 26 6 0.00% 27 9 0.00% 28 6 0.00% 29 9 0.00% 30 10 0.00% 31 13 0.00% 32 16 0.00% 33 23 0.00% 34 24 0.00% 35 37 0.00% 36 18 0.00% 37 22 0.00% 38 31 0.00% 39 30 0.00% 40 35 0.00% 41 44 0.00% 42 31 0.00% 43 54 0.00% 44 47 0.00% 45 50 0.00% 46 65 0.00% 47 85 0.00% 48 74 0.00% 49 90 0.00% 50 103 0.00% 51 139 0.00% 52 173 0.00% 53 155 0.00% 54 168 0.00% 55 199 0.00% 56 221 0.00% 57 236 0.00% 58 295 0.00% 59 358 0.00% 60 367 0.00% 61 441 0.00% 62 554 0.00% 63 623 0.00% 64 686 0.00% 65 747 0.01% 66 788 0.01% 67 865 0.01% 68 946 0.01% 69 1136 0.01% 70 1324 0.01% 71 1590 0.01% 72 1830 0.01% 73 2095 0.01% 74 2228 0.02% 75 2483 0.02% 76 2635 0.02% 77 2958 0.02% 78 3109 0.02% 79 3514 0.03% 80 3851 0.03% 81 4524 0.03% 82 5105 0.04% 83 5686 0.04% 84 6385 0.05% 85 6973 0.05% 86 7299 0.05% 87 7566 0.05% 88 7944 0.06% 89 8520 0.06% 90 9344 0.07% 91 10350 0.07% 92 11194 0.08% 93 12514 0.09% 94 13171 0.09% 95 13571 0.10% 96 14486 0.10% 97 14500 0.10% 98 14890 0.11% 99 15542 0.11% 100 16434 0.12% 101 17502 0.12% 102 18989 0.14% 103 20208 0.14% 104 21345 0.15% 105 22105 0.16% 106 22896 0.16% 107 22800 0.16% 108 23111 0.16% 109 23037 0.16% 110 23894 0.17% 111 25208 0.18% 112 26557 0.19% 113 28332 0.20% 114 29380 0.21% 115 30631 0.22% 116 30737 0.22% 117 31082 0.22% 118 30947 0.22% 119 30898 0.22% 120 31621 0.23% 121 32360 0.23% 122 34231 0.24% 123 35542 0.25% 124 37721 0.27% 125 38596 0.27% 126 39430 0.28% 127 39362 0.28% 128 39123 0.28% 129 38814 0.28% 130 39484 0.28% 131 39871 0.28% 132 41386 0.29% 133 43413 0.31% 134 44834 0.32% 135 46943 0.33% 136 47214 0.34% 137 47022 0.33% 138 46711 0.33% 139 46607 0.33% 140 46261 0.33% 141 46746 0.33% 142 48438 0.34% 143 50211 0.36% 144 51804 0.37% 145 53705 0.38% 146 54318 0.39% 147 54624 0.39% 148 54464 0.39% 149 53701 0.38% 150 54384 0.39% 151 11943208 85.03% 14045490 reads passed initial QC criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=39 prefix-density=0.21 prefix-fanout=2.0 sequence=CCAACATACCAGTGCACAAACGCACGCTTGGCATACATGAGATCAAACTTGTGGTCAATGCGAGAGAA criterion=fanout-score sequence-density=0.02 sequence-density-rank=43 fanout-score=47.56 fanout-score-rank=1 prefix-density=0.16 prefix-fanout=6.3 sequence=AACAATCTTACATCAAATTACAAGCACGTATGGTCTTGTAATATTTGCAGTAAACCGAGCTTTTTTTTCTAAAAAGGAAGAAAAACAGTAGATGGACATAACCAAACAAGCCACACATCAAGCATCATCATCACCGTTCTATAGAACACAAGAATACTGCCTGCTGCCCTACTGGGAAGCACTCTCCTTTTCTTTCTCCTTCTCTTCTTCAGTCTTGGGGTGGTACCCAGGTAACTTCTCCTTGATCTTCTCGAG criterion=sequence-density sequence-density=0.19 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=42 prefix-density=0.20 prefix-fanout=2.0 sequence=TTCTCTCGCATTGACCACAAGTTTGATCTCATGTATGCCAAGCGTGCGTTTGTGCACTGGTATGTTGG criterion=fanout-score sequence-density=0.04 sequence-density-rank=42 fanout-score=146.70 fanout-score-rank=1 prefix-density=0.42 prefix-fanout=13.8 sequence=AGAAAGAAATGAGAATTCTCATGGTGGGTCTTGATGCTGCTGGTAAGACCACCATCTTGTACAAGCTCAA SRR7170015 testing PE reads STAR mapping to Ensembl genome Started job on | Apr 15 04:23:59 Started mapping on | Apr 15 04:23:59 Finished on | Apr 15 04:26:03 Mapping speed, Million of reads per hour | 407.77 Number of input reads | 14045490 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 12893538 Uniquely mapped reads % | 91.80% Average mapped length | 292.89 Number of splices: Total | 12281164 Number of splices: Annotated (sjdb) | 12080066 Number of splices: GT/AG | 12101818 Number of splices: GC/AG | 142691 Number of splices: AT/AC | 10930 Number of splices: Non-canonical | 25725 Mismatch rate per base, % | 0.34% Deletion rate per base | 0.02% Deletion average length | 2.71 Insertion rate per base | 0.02% Insertion average length | 2.46 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 239549 % of reads mapped to multiple loci | 1.71% Number of reads mapped to too many loci | 22028 % of reads mapped to too many loci | 0.16% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.30% % of reads unmapped: other | 0.04% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 912403 912403 912403 N_multimapping 239549 239549 239549 N_noFeature 230824 12744388 295259 N_ambiguous 131907 637 46774 UnstrandedReadsAssigned:12530807 PositiveStrandReadsAssigned:148513 NegativeStrandReadsAssigned:12551505 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR7170015 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR7170015-trimmed-pair1.fastq SRR7170015-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 14,045,490 reads, 12,587,074 reads pseudoaligned [quant] estimated average fragment length: 220.122 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,078 rounds 52401 SRR7170015.ke.tsv 34699 SRR7170015.se.tsv 87100 total ==> SRR7170015.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1798.88 249 10.4116 Potri.005G024800.1.v4.1 1035 815.878 36 3.31893 Potri.004G059700.1.v4.1 961 741.884 7 0.709713 Potri.007G009000.2.v4.1 1416 1196.88 0 0 Potri.003G141000.2.v4.1 2943 2723.88 225.031 6.21405 Potri.016G087400.1.v4.1 270 90.121 1354 1130.09 Potri.015G069301.1.v4.1 564 347.664 0 0 Potri.010G195200.1.v4.1 1773 1553.88 7 0.338845 Potri.012G127500.1.v4.1 977 757.878 3975 394.51 ==> SRR7170015.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 707 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 237 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 4 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 1 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 3 SRR7170015 completed mapping pipeline successfully