Starting /dee2/code/volunteer_pipeline.sh SRR7171051 current disk space = 3088529629184 free memory = 1410709116 SRR7171051 SRAfilesize f41a4ed96adbf6ec0ff11b3551522253 SRR7171051.sra SRR7171051.sra file validated SRR7171051 is paired end SRR7171051 is conventional basespace SRR7171051 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7171051_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.92475 32.0 25.0 33.0 18.0 34.0 2 30.994 33.0 31.0 33.0 27.0 34.0 3 32.3375 33.0 33.0 33.0 30.0 34.0 4 32.8345 33.0 33.0 34.0 31.0 34.0 5 33.307 34.0 33.0 34.0 33.0 34.0 6 37.21125 38.0 37.0 38.0 36.0 38.0 7 37.52225 38.0 38.0 38.0 37.0 38.0 8 37.6095 38.0 38.0 38.0 38.0 38.0 9 37.69525 38.0 38.0 38.0 38.0 38.0 10-14 37.65375 38.0 38.0 38.0 38.0 38.0 15-19 37.60189999999999 38.0 38.0 38.0 38.0 38.0 20-24 37.553999999999995 38.0 38.0 38.0 38.0 38.0 25-29 37.5065 38.0 38.0 38.0 38.0 38.0 30-34 37.49635 38.0 38.0 38.0 38.0 38.0 35-39 37.3051 38.0 38.0 38.0 36.8 38.0 40-44 37.3527 38.0 38.0 38.0 37.2 38.0 45-49 37.419 38.0 38.0 38.0 37.0 38.0 50-54 36.727850000000004 38.0 38.0 38.0 34.0 38.0 55-59 37.0447 38.0 38.0 38.0 36.0 38.0 60-64 37.06135 38.0 38.0 38.0 36.0 38.0 65-69 37.055699999999995 38.0 38.0 38.0 36.0 38.0 70-74 36.882000000000005 38.0 38.0 38.0 35.8 38.0 75-79 36.1509 38.0 38.0 38.0 34.4 38.0 80-84 35.8874 38.0 38.0 38.0 34.0 38.0 85-89 35.736900000000006 38.0 38.0 38.0 33.6 38.0 90-94 35.49635 38.0 37.6 38.0 32.2 38.0 95-99 35.5252 38.0 37.8 38.0 32.8 38.0 100-104 35.4867 38.0 37.6 38.0 32.4 38.0 105-109 35.2657 38.0 37.0 38.0 31.2 38.0 110-114 35.04635 38.0 36.8 38.0 29.6 38.0 115-119 34.75085 38.0 36.0 38.0 28.4 38.0 120-124 34.672900000000006 38.0 36.0 38.0 27.8 38.0 125-129 34.44765 38.0 35.6 38.0 26.8 38.0 130-134 31.309749999999998 36.0 27.8 38.0 17.4 38.0 135-139 33.64880000000001 38.0 34.0 38.0 21.8 38.0 140-144 33.2405 38.0 33.6 38.0 17.4 38.0 145-149 32.47485 38.0 33.0 38.0 13.0 38.0 150-151 28.221874999999997 34.5 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 2.0 8 1.0 9 0.0 10 0.0 11 3.0 12 2.0 13 4.0 14 2.0 15 4.0 16 5.0 17 7.0 18 16.0 19 78.0 20 8.0 21 5.0 22 7.0 23 10.0 24 11.0 25 8.0 26 12.0 27 10.0 28 28.0 29 26.0 30 36.0 31 40.0 32 83.0 33 93.0 34 171.0 35 317.0 36 1046.0 37 1964.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.84755174154468 11.256940938919739 13.099444724886421 33.796062594649165 2 20.455113778444613 16.25406351587897 35.13378344586147 28.157039259814955 3 18.525 21.55 30.575000000000003 29.349999999999998 4 20.849999999999998 26.625 24.775 27.750000000000004 5 22.725 32.125 25.575 19.575 6 18.5 32.95 28.349999999999998 20.200000000000003 7 13.850000000000001 23.375 44.425 18.35 8 16.475 26.1 32.5 24.925 9 18.7 23.275000000000002 32.800000000000004 25.224999999999998 10-14 19.665 29.34 25.874999999999996 25.119999999999997 15-19 20.075000000000003 28.42 26.895000000000003 24.610000000000003 20-24 20.355 28.875 26.91 23.86 25-29 19.91 28.189999999999998 27.29 24.610000000000003 30-34 19.2 27.944999999999997 27.3 25.555 35-39 19.470000000000002 28.77 27.025 24.735 40-44 19.455 28.01 27.88 24.654999999999998 45-49 20.625 27.865000000000002 26.950000000000003 24.560000000000002 50-54 21.099999999999998 28.52 26.19 24.19 55-59 18.85 27.725 28.249999999999996 25.174999999999997 60-64 19.54 26.75 28.88 24.83 65-69 20.07 29.665000000000003 26.0 24.265 70-74 19.205 30.375000000000004 25.924999999999997 24.495 75-79 19.53 30.335 25.4 24.735 80-84 19.91 29.86 26.095000000000002 24.135 85-89 20.305 29.01 25.635 25.05 90-94 20.335 28.74 25.835 25.09 95-99 20.575 27.889999999999997 26.445 25.09 100-104 20.7 28.494999999999997 25.645 25.16 105-109 20.69 28.365000000000002 25.629999999999995 25.314999999999998 110-114 20.78 28.37 25.965 24.884999999999998 115-119 21.2 28.799999999999997 25.445 24.555 120-124 20.330000000000002 29.220000000000002 24.48 25.97 125-129 20.895 28.244999999999997 25.655 25.205 130-134 20.485 29.115000000000002 25.19 25.21 135-139 21.065 28.21 25.39 25.335 140-144 21.55 27.875 23.915 26.66 145-149 20.77 27.875 24.725 26.63 150-151 19.275000000000002 28.5875 25.8 26.337500000000002 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 2.0 3 1.5 4 0.0 5 0.0 6 0.5 7 2.5 8 2.5 9 0.5 10 0.0 11 0.5 12 0.5 13 0.5 14 0.5 15 0.0 16 0.0 17 1.0 18 2.0 19 1.0 20 0.5 21 0.5 22 1.5 23 4.0 24 5.5 25 8.5 26 13.0 27 19.0 28 24.0 29 25.5 30 31.5 31 48.0 32 60.5 33 72.0 34 88.0 35 100.5 36 117.5 37 135.0 38 139.5 39 141.5 40 154.5 41 157.5 42 161.5 43 170.5 44 178.0 45 173.5 46 152.5 47 147.5 48 139.0 49 133.5 50 141.5 51 139.0 52 141.5 53 162.0 54 170.5 55 146.5 56 109.0 57 90.0 58 80.0 59 65.5 60 50.0 61 36.0 62 27.0 63 13.5 64 3.5 65 0.5 66 0.0 67 1.5 68 2.0 69 0.5 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.95 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.275 #Duplication Level Percentage of deduplicated Percentage of total 1 94.53412630982724 83.45 2 3.3701500991220614 5.949999999999999 3 0.8779382611158312 2.325 4 0.5947323704333051 2.1 5 0.16992353440951571 0.75 6 0.08496176720475786 0.44999999999999996 7 0.11328235627301048 0.7000000000000001 8 0.05664117813650524 0.4 9 0.02832058906825262 0.22499999999999998 >10 0.1416029453412631 1.4500000000000002 >50 0.02832058906825262 2.1999999999999997 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 88 2.1999999999999997 TruSeq Adapter, Index 2 (97% over 37bp) CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG 15 0.375 No Hit GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG 12 0.3 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG 11 0.27499999999999997 No Hit GTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCA 10 0.25 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT 10 0.25 No Hit GGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCA 9 0.22499999999999998 No Hit GTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCG 8 0.2 No Hit GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA 8 0.2 No Hit CCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGC 7 0.17500000000000002 No Hit GTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACA 7 0.17500000000000002 No Hit GGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTT 7 0.17500000000000002 No Hit CGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC 7 0.17500000000000002 No Hit CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG 6 0.15 No Hit GTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCAC 6 0.15 No Hit CCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAC 5 0.125 TruSeq Adapter, Index 2 (97% over 37bp) GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC 5 0.125 No Hit GTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAG 5 0.125 No Hit CGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA 5 0.125 No Hit GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG 5 0.125 No Hit CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.0625 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.0875 0.0 0.0 0.0 0.0 62-63 0.1125 0.0 0.0 0.0 0.0 64-65 0.1375 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.175 0.0 0.0 0.0 0.0 70-71 0.225 0.0 0.0 0.0 0.0 72-73 0.2625 0.0 0.0 0.0 0.0 74-75 0.2875 0.0 0.0 0.0 0.0 76-77 0.375 0.0 0.0 0.0 0.0 78-79 0.425 0.0 0.0 0.0 0.0 80-81 0.4875 0.0 0.0 0.0 0.0 82-83 0.5375 0.0 0.0 0.0 0.0 84-85 0.7625 0.0 0.0 0.0 0.0 86-87 0.975 0.0 0.0 0.0 0.0 88-89 1.2374999999999998 0.0 0.0 0.0 0.0 90-91 1.5375 0.0 0.0 0.0 0.0 92-93 1.8 0.0 0.0 0.0 0.0 94-95 2.1125 0.0 0.0 0.0 0.0 96-97 2.5375 0.0 0.0 0.0 0.0 98-99 2.8125 0.0 0.0 0.0 0.0 100-101 3.25 0.0 0.0 0.0 0.0 102-103 3.6500000000000004 0.0 0.0 0.0 0.0 104-105 4.0625 0.0 0.0 0.0 0.0 106-107 4.725 0.0 0.0 0.0 0.0 108-109 5.35 0.0 0.0 0.0 0.0 110-111 6.2375 0.0 0.0 0.0 0.0 112-113 6.9375 0.0 0.0 0.0 0.0 114-115 7.475 0.0 0.0 0.0 0.0 116-117 8.1625 0.0 0.0 0.0 0.0 118-119 9.05 0.0 0.0 0.0 0.0 120-121 9.775 0.0 0.0 0.0 0.0 122-123 10.3 0.0 0.0 0.0 0.0 124-125 11.0125 0.0 0.0 0.0 0.0 126-127 11.6375 0.0 0.0 0.0 0.0 128-129 12.475 0.0 0.0 0.0 0.0 130-131 13.225000000000001 0.0 0.0 0.0 0.0 132-133 14.5 0.0 0.0 0.0 0.0 134-135 15.537500000000001 0.0 0.0 0.0 0.0 136-137 16.5625 0.0 0.0 0.0 0.0 138-139 17.45 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACAA 10 0.006832588 144.9875 145 CTTCTGC 40 0.0076588374 36.246876 145 GAAGAGC 125 4.3056428E-4 28.997501 6 GAGCACA 125 4.3056428E-4 28.997501 9 AAGAGCA 130 5.4183323E-4 27.882212 7 CGGAAGA 130 5.4183323E-4 27.882212 4 AGAGCAC 130 5.4183323E-4 27.882212 8 GGAAGAG 130 5.4183323E-4 27.882212 5 GATCGGA 145 9.514085E-4 25.314274 1 TCGGAAG 145 0.0010263528 24.997845 3 ATCGGAA 145 0.0010263528 24.997845 2 TTCTGCT 30 0.0014445208 24.164585 55-59 CTGCTTG 30 0.0014445208 24.164585 55-59 GCTTGAA 35 0.003538379 20.974684 1 TCTGCTT 35 0.003538379 20.7125 55-59 TGCTTGA 35 0.003538379 20.7125 60-64 TGCCGTC 40 0.0076588374 18.123438 50-54 GAAAAAA 40 0.0076588374 18.123438 65-69 CCGTCTT 40 0.0076588374 18.123438 50-54 ATGCCGT 40 0.0076588374 18.123438 45-49 >>END_MODULE SRR7171051 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7171051_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.87225 33.0 33.0 34.0 32.0 34.0 2 32.41 33.0 33.0 34.0 31.0 34.0 3 32.86725 34.0 33.0 34.0 32.0 34.0 4 32.939 34.0 33.0 34.0 32.0 34.0 5 33.00625 34.0 33.0 34.0 32.0 34.0 6 37.156 38.0 38.0 38.0 37.0 38.0 7 37.18875 38.0 38.0 38.0 37.0 38.0 8 37.24375 38.0 38.0 38.0 37.0 38.0 9 37.2415 38.0 38.0 38.0 37.0 38.0 10-14 37.1946 38.0 38.0 38.0 37.0 38.0 15-19 37.07430000000001 38.0 38.0 38.0 36.8 38.0 20-24 35.7278 38.0 37.0 38.0 29.0 38.0 25-29 36.6142 38.0 38.0 38.0 34.6 38.0 30-34 36.9702 38.0 38.0 38.0 36.4 38.0 35-39 37.12134999999999 38.0 38.0 38.0 37.0 38.0 40-44 37.14165 38.0 38.0 38.0 37.0 38.0 45-49 37.0304 38.0 38.0 38.0 36.6 38.0 50-54 37.0841 38.0 38.0 38.0 36.8 38.0 55-59 36.909000000000006 38.0 38.0 38.0 36.2 38.0 60-64 36.81635 38.0 38.0 38.0 36.0 38.0 65-69 36.83565 38.0 38.0 38.0 35.8 38.0 70-74 36.8069 38.0 38.0 38.0 35.8 38.0 75-79 36.44500000000001 38.0 37.8 38.0 33.8 38.0 80-84 34.2664 37.8 34.4 38.0 26.6 38.0 85-89 35.817049999999995 38.0 38.0 38.0 34.0 38.0 90-94 35.7458 38.0 38.0 38.0 34.0 38.0 95-99 35.6161 38.0 38.0 38.0 33.2 38.0 100-104 35.47225 38.0 38.0 38.0 33.0 38.0 105-109 34.06725 37.8 35.0 38.0 26.4 38.0 110-114 34.8512 38.0 36.4 38.0 29.0 38.0 115-119 34.168949999999995 38.0 35.2 38.0 24.4 38.0 120-124 34.41985 38.0 35.6 38.0 26.0 38.0 125-129 34.1862 38.0 35.0 38.0 24.4 38.0 130-134 33.902300000000004 38.0 35.0 38.0 23.2 38.0 135-139 33.40725 38.0 33.0 38.0 20.2 38.0 140-144 31.138349999999996 36.6 28.6 38.0 13.4 38.0 145-149 29.50615 34.6 27.6 38.0 2.0 38.0 150-151 25.511375 32.0 15.0 37.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 4.0 4 0.0 5 1.0 6 0.0 7 1.0 8 2.0 9 1.0 10 3.0 11 5.0 12 4.0 13 1.0 14 2.0 15 9.0 16 10.0 17 7.0 18 19.0 19 42.0 20 45.0 21 2.0 22 11.0 23 9.0 24 10.0 25 14.0 26 14.0 27 16.0 28 37.0 29 42.0 30 43.0 31 48.0 32 90.0 33 131.0 34 197.0 35 377.0 36 1043.0 37 1754.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.2 19.35 12.6 23.849999999999998 2 29.075 24.474999999999998 28.775000000000002 17.675 3 25.0 24.525 32.574999999999996 17.9 4 26.563281640820406 32.06603301650826 21.010505252626313 20.36018009004502 5 29.48974487243622 34.642321160580295 19.15957978989495 16.708354177088545 6 24.6 36.65 20.9 17.849999999999998 7 21.2 21.349999999999998 37.45 20.0 8 23.925 26.05 26.5 23.525 9 26.25 23.95 26.200000000000003 23.599999999999998 10-14 26.455000000000002 27.639999999999997 24.495 21.41 15-19 27.005000000000003 25.624999999999996 26.845000000000002 20.525 20-24 26.455000000000002 27.139999999999997 25.985000000000003 20.419999999999998 25-29 25.765 27.639999999999997 26.41 20.185 30-34 26.185000000000002 26.895000000000003 26.96 19.96 35-39 26.255 26.77 26.545 20.43 40-44 26.025 26.605 27.065 20.305 45-49 25.674999999999997 27.49 26.39 20.445 50-54 25.929999999999996 26.700000000000003 27.08 20.29 55-59 26.325 26.229999999999997 26.974999999999998 20.47 60-64 25.130000000000003 26.05 27.355 21.465 65-69 25.314999999999998 26.35 28.139999999999997 20.195 70-74 24.995 29.425 26.095000000000002 19.485 75-79 25.185000000000002 28.084999999999997 26.484999999999996 20.244999999999997 80-84 25.490000000000002 28.105000000000004 27.279999999999998 19.125 85-89 25.28 28.449999999999996 26.400000000000002 19.869999999999997 90-94 25.505 27.985 26.674999999999997 19.835 95-99 25.14 28.115000000000002 26.735 20.01 100-104 26.245 27.79 26.44 19.525000000000002 105-109 25.52 27.625 26.85 20.005 110-114 26.52 28.125 26.07 19.285 115-119 26.095000000000002 28.675 26.200000000000003 19.03 120-124 26.445 28.804999999999996 25.55 19.2 125-129 26.705000000000002 28.810000000000002 25.674999999999997 18.81 130-134 27.155 28.565 25.155 19.125 135-139 27.584999999999997 27.965 25.814999999999998 18.634999999999998 140-144 27.47 27.63 25.855 19.045 145-149 28.215 26.915 26.295 18.575 150-151 28.875 27.375 25.724999999999998 18.025 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.0 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 2.0 21 1.5 22 0.5 23 1.5 24 3.0 25 4.5 26 6.5 27 6.0 28 7.5 29 12.0 30 15.5 31 21.0 32 31.5 33 43.0 34 54.0 35 66.0 36 76.0 37 88.5 38 104.0 39 132.5 40 155.0 41 167.5 42 174.0 43 185.0 44 194.0 45 187.0 46 198.0 47 192.0 48 183.5 49 180.0 50 167.5 51 158.0 52 148.5 53 173.0 54 198.0 55 177.5 56 131.5 57 96.0 58 74.5 59 57.0 60 40.0 61 28.5 62 27.0 63 16.5 64 3.0 65 0.5 66 0.5 67 1.5 68 1.5 69 0.5 70 0.5 71 0.5 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.05 5 0.05 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.375 #Duplication Level Percentage of deduplicated Percentage of total 1 95.49100968188105 86.3 2 2.793914246196404 5.050000000000001 3 0.7468879668049793 2.025 4 0.5255878284923928 1.9 5 0.19363762102351315 0.8750000000000001 6 0.05532503457814661 0.3 7 0.027662517289073305 0.17500000000000002 8 0.027662517289073305 0.2 9 0.027662517289073305 0.22499999999999998 >10 0.08298755186721991 1.0250000000000001 >50 0.027662517289073305 1.925 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT 77 1.925 Illumina Single End PCR Primer 1 (96% over 32bp) CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA 17 0.42500000000000004 No Hit CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG 12 0.3 No Hit GCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAA 12 0.3 No Hit GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG 9 0.22499999999999998 No Hit GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA 8 0.2 No Hit GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA 7 0.17500000000000002 No Hit GCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAG 6 0.15 No Hit CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG 6 0.15 No Hit GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGC 5 0.125 No Hit GCTGGTTCTCCCCGAAAGCTATTTAGGTAGCGCCTCGTGAATTCATCTCC 5 0.125 No Hit GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC 5 0.125 No Hit GGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGC 5 0.125 No Hit CGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCT 5 0.125 No Hit CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT 5 0.125 No Hit GCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.037500000000000006 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.0625 0.0 0.0 0.0 0.0 62-63 0.0875 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.175 0.0 0.0 0.0 0.0 70-71 0.2375 0.0 0.0 0.0 0.0 72-73 0.275 0.0 0.0 0.0 0.0 74-75 0.2875 0.0 0.0 0.0 0.0 76-77 0.35 0.0 0.0 0.0 0.0 78-79 0.3875 0.0 0.0 0.0 0.0 80-81 0.4625 0.0 0.0 0.0 0.0 82-83 0.5125 0.0 0.0 0.0 0.0 84-85 0.7250000000000001 0.0 0.0 0.0 0.0 86-87 0.925 0.0 0.0 0.0 0.0 88-89 1.1875 0.0 0.0 0.0 0.0 90-91 1.45 0.0 0.0 0.0 0.0 92-93 1.7125 0.0 0.0 0.0 0.0 94-95 2.0250000000000004 0.0 0.0 0.0 0.0 96-97 2.45 0.0 0.0 0.0 0.0 98-99 2.7249999999999996 0.0 0.0 0.0 0.0 100-101 3.1375 0.0 0.0 0.0 0.0 102-103 3.4875 0.0 0.0 0.0 0.0 104-105 3.85 0.0 0.0 0.0 0.0 106-107 4.425000000000001 0.0 0.0 0.0 0.0 108-109 4.987500000000001 0.0 0.0 0.0 0.0 110-111 5.8375 0.0 0.0 0.0 0.0 112-113 6.5 0.0 0.0 0.0 0.0 114-115 6.975 0.0 0.0 0.0 0.0 116-117 7.675000000000001 0.0 0.0 0.0 0.0 118-119 8.6 0.0 0.0 0.0 0.0 120-121 9.4375 0.0 0.0 0.0 0.0 122-123 10.024999999999999 0.0 0.0 0.0 0.0 124-125 10.825 0.0 0.0 0.0 0.0 126-127 11.524999999999999 0.0 0.0 0.0 0.0 128-129 12.537500000000001 0.0 0.0 0.0 0.0 130-131 13.5125 0.0 0.0 0.0 0.0 132-133 14.7125 0.0 0.0 0.0 0.0 134-135 15.575 0.0 0.0 0.0 0.0 136-137 16.4625 0.0 0.0 0.0 0.0 138-139 17.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACTCAA 10 0.006830828 145.0 5 ACTCAAA 10 0.006830828 145.0 6 GAACTCA 10 0.006830828 145.0 4 CGGGAAC 10 0.006830828 145.0 1 ATCACCT 10 0.006830828 145.0 6 GGAACTC 10 0.006830828 145.0 3 GTCGCCG 40 0.0076550315 36.25 145 AAGAGCG 125 9.7948E-6 34.8 7 GAAGAGC 130 1.2818533E-5 33.461536 6 GAGCGTC 120 3.3870232E-4 30.208334 9 AGAGCGT 120 3.3870232E-4 30.208334 8 GTATCAT 25 4.977651E-4 29.0 60-64 CCGTATC 25 4.977651E-4 29.0 60-64 TCGCCGT 25 4.977651E-4 29.0 55-59 ATTAAAA 25 4.977651E-4 29.0 65-69 CGTATCA 25 4.977651E-4 29.0 60-64 ATCATTA 25 4.977651E-4 29.0 60-64 GCCGTAT 25 4.977651E-4 29.0 55-59 TCATTAA 25 4.977651E-4 29.0 65-69 CGCCGTA 25 4.977651E-4 29.0 55-59 >>END_MODULE Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758302 spots for SRR7171051.sra Written 758302 spots for SRR7171051.sra Read 758309 spots for SRR7171051.sra Written 758309 spots for SRR7171051.sra SRR ids: ['SRR7171051.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_6xklht2e SRR7171051.sra spots: 15166047 blocks: [[1, 758302], [758303, 1516604], [1516605, 2274906], [2274907, 3033208], [3033209, 3791510], [3791511, 4549812], [4549813, 5308114], [5308115, 6066416], [6066417, 6824718], [6824719, 7583020], [7583021, 8341322], [8341323, 9099624], [9099625, 9857926], [9857927, 10616228], [10616229, 11374530], [11374531, 12132832], [12132833, 12891134], [12891135, 13649436], [13649437, 14407738], [14407739, 15166047]] SRR7171051 file size 5117575 SRR7171051 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7171051 SRR7171051_1.fastq SRR7171051_2.fastq Input file: SRR7171051_1.fastq Paired file: SRR7171051_2.fastq trimmed: SRR7171051-trimmed-pair1.fastq, SRR7171051-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Thu Feb 13 21:57:01 2025 >> started Thu Feb 13 21:57:18 2025 >> done (17.390s) 15166047 read pairs processed; of these: 24380 ( 0.16%) short read pairs filtered out after trimming by size control 387151 ( 2.55%) empty read pairs filtered out after trimming by size control 14754516 (97.29%) read pairs available; of these: 9815783 (66.53%) trimmed read pairs available after processing 4938733 (33.47%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 18 0.00% 19 30 0.00% 20 29 0.00% 21 37 0.00% 22 31 0.00% 23 29 0.00% 24 33 0.00% 25 34 0.00% 26 36 0.00% 27 41 0.00% 28 41 0.00% 29 38 0.00% 30 52 0.00% 31 63 0.00% 32 52 0.00% 33 38 0.00% 34 50 0.00% 35 61 0.00% 36 65 0.00% 37 84 0.00% 38 81 0.00% 39 92 0.00% 40 120 0.00% 41 122 0.00% 42 134 0.00% 43 155 0.00% 44 158 0.00% 45 217 0.00% 46 266 0.00% 47 318 0.00% 48 308 0.00% 49 420 0.00% 50 485 0.00% 51 496 0.00% 52 519 0.00% 53 491 0.00% 54 533 0.00% 55 575 0.00% 56 637 0.00% 57 717 0.00% 58 848 0.01% 59 1000 0.01% 60 1084 0.01% 61 1202 0.01% 62 1432 0.01% 63 1616 0.01% 64 1684 0.01% 65 1871 0.01% 66 1920 0.01% 67 2116 0.01% 68 2282 0.02% 69 2483 0.02% 70 3072 0.02% 71 3537 0.02% 72 4341 0.03% 73 4922 0.03% 74 6146 0.04% 75 9888 0.07% 76 21203 0.14% 77 28093 0.19% 78 14891 0.10% 79 10661 0.07% 80 10185 0.07% 81 10625 0.07% 82 11793 0.08% 83 13757 0.09% 84 17214 0.12% 85 17261 0.12% 86 19229 0.13% 87 20366 0.14% 88 23827 0.16% 89 23734 0.16% 90 24829 0.17% 91 27236 0.18% 92 26935 0.18% 93 32827 0.22% 94 33986 0.23% 95 37171 0.25% 96 36815 0.25% 97 36507 0.25% 98 37336 0.25% 99 39240 0.27% 100 45190 0.31% 101 41539 0.28% 102 44311 0.30% 103 46506 0.32% 104 50671 0.34% 105 57326 0.39% 106 54408 0.37% 107 52469 0.36% 108 55783 0.38% 109 63999 0.43% 110 65015 0.44% 111 59550 0.40% 112 62213 0.42% 113 73187 0.50% 114 67759 0.46% 115 73929 0.50% 116 75180 0.51% 117 70108 0.48% 118 73178 0.50% 119 72709 0.49% 120 75965 0.51% 121 73799 0.50% 122 77484 0.53% 123 82667 0.56% 124 83447 0.57% 125 82914 0.56% 126 82291 0.56% 127 84216 0.57% 128 86442 0.59% 129 86893 0.59% 130 90053 0.61% 131 90558 0.61% 132 93699 0.64% 133 98349 0.67% 134 104703 0.71% 135 110304 0.75% 136 110163 0.75% 137 118273 0.80% 138 120947 0.82% 139 125355 0.85% 140 127505 0.86% 141 141725 0.96% 142 145021 0.98% 143 158454 1.07% 144 177025 1.20% 145 204731 1.39% 146 242402 1.64% 147 310612 2.11% 148 457017 3.10% 149 848096 5.75% 150 3284772 22.26% 151 4938733 33.47% 14754516 reads passed initial QC criterion=sequence-density sequence-density=0.58 sequence-density-rank=1 fanout-score=2.62 fanout-score-rank=38 prefix-density=1.49 prefix-fanout=1.0 sequence=CACTCGTCAGCGAAACAGCAAGCTGTTTCCTGTTACC criterion=fanout-score sequence-density=0.02 sequence-density-rank=43 fanout-score=200.97 fanout-score-rank=1 prefix-density=0.21 prefix-fanout=14.6 sequence=AAAAGGAAAAGCAACGATCTTTTTGCCAGAGCCCAGGTACAATTTGAACAAAGCAACCCTAACAGATAGCTAGGGACTCATCAAATCTTGGAACCTAGACACCCTTCGGCTTGGAGGCGATAAAACTGATGCACTGCACTTGACGAGTGTTGTCGAATCCAATGATACGGATAAAGGAGTTAGGGTAAGCTTTCTTCGCCTCCTCGAGCTCAATCAGCACCTGAGATGCCTCAGTGCATCCAAACATGGGTAGTTTCCACATAGTCCAGTAGCGTCCATCATAGTACCCTGGGGACTGGTGGTGCTCGCGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTGTTGCGAAGAAGGTACTCAATTTCCTGGGCCAATTGCTCAGTAGTGAGATCTGGAAGGTAAGAAAGAGTCTCGAACTTCTTCAATCCAGTTGGAGGCCACACCTGCATGCATTGAACTCTTCCGCCATTGCTTGCAATGGAAGTAATGTCATT criterion=sequence-density sequence-density=2.43 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=37 prefix-density=2.47 prefix-fanout=2.0 sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG criterion=fanout-score sequence-density=0.11 sequence-density-rank=31 fanout-score=13.79 fanout-score-rank=1 prefix-density=0.31 prefix-fanout=4.7 sequence=GAAGGCTGTACCGACCCAAATGCAGCAAACTTTGATCCAACAGCTAGGAGTGATGATGGAAGCTGCTCTTACTGAATCCTTTTCTAGCTTATTCGGTGTGCAAGGAGCAGCACTTTCTAAGTCATGATGTGAGATAACCAAATCCTATTGTTCCATTTTCTTAAATGGATATTGCTATCCTCTGAT SRR7171051 testing PE reads STAR mapping to Ensembl genome Unpaired reads removal Started job on | Feb 13 22:06:36 Started mapping on | Feb 13 22:06:36 Finished on | Feb 13 22:10:33 Mapping speed, Million of reads per hour | 223.91 Number of input reads | 14740940 Average input read length | 262 UNIQUE READS: Uniquely mapped reads number | 7621010 Uniquely mapped reads % | 51.70% Average mapped length | 269.30 Number of splices: Total | 5093658 Number of splices: Annotated (sjdb) | 4947761 Number of splices: GT/AG | 4965690 Number of splices: GC/AG | 89557 Number of splices: AT/AC | 3914 Number of splices: Non-canonical | 34497 Mismatch rate per base, % | 0.47% Deletion rate per base | 0.04% Deletion average length | 2.69 Insertion rate per base | 0.03% Insertion average length | 2.00 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 268960 % of reads mapped to multiple loci | 1.82% Number of reads mapped to too many loci | 37882 % of reads mapped to too many loci | 0.26% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 45.88% % of reads unmapped: other | 0.34% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 6868547 6868547 6868547 N_multimapping 268960 268960 268960 N_noFeature 230350 7447276 273249 N_ambiguous 313165 2162 181024 UnstrandedReadsAssigned:7077495 PositiveStrandReadsAssigned:171572 NegativeStrandReadsAssigned:7166737 Dataset is classified negative stranded MeadianReadLen=131 20thPercentileLength=114 echo kmer=109 SRR7171051 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR7171051-trimmed-pair1.fastq SRR7171051-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 14,740,940 reads, 9,747,176 reads pseudoaligned [quant] estimated average fragment length: 175.663 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,084 rounds 52401 SRR7171051.ke.tsv 34699 SRR7171051.se.tsv 87100 total ==> SRR7171051.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1843.34 188 9.40281 Potri.005G024800.1.v4.1 1035 860.337 94 10.0731 Potri.004G059700.1.v4.1 961 786.342 4 0.468979 Potri.007G009000.2.v4.1 1416 1241.34 0 0 Potri.003G141000.2.v4.1 2943 2768.34 310.641 10.3453 Potri.016G087400.1.v4.1 270 115.374 377.447 301.615 Potri.015G069301.1.v4.1 564 389.997 0 0 Potri.010G195200.1.v4.1 1773 1598.34 17 0.980585 Potri.012G127500.1.v4.1 977 802.337 10 1.14907 ==> SRR7171051.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 203 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 281 Potri.001G212900.v4.1 29 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 3 SRR7171051 completed mapping pipeline successfully