Starting /dee2/code/volunteer_pipeline.sh SRR7171060 current disk space = 2796454658048 free memory = 1559646260 SRR7171060_1.fastq is conventional basespace SRR7171060_1.fastq read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7171060_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13419171 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0 30.0 30.0 30.0 30.0 30.0 2 30.0 30.0 30.0 30.0 30.0 30.0 3 30.0 30.0 30.0 30.0 30.0 30.0 4 30.0 30.0 30.0 30.0 30.0 30.0 5 30.0 30.0 30.0 30.0 30.0 30.0 6 30.0 30.0 30.0 30.0 30.0 30.0 7 30.0 30.0 30.0 30.0 30.0 30.0 8 30.0 30.0 30.0 30.0 30.0 30.0 9 30.0 30.0 30.0 30.0 30.0 30.0 10-14 30.0 30.0 30.0 30.0 30.0 30.0 15-19 30.0 30.0 30.0 30.0 30.0 30.0 20-24 30.0 30.0 30.0 30.0 30.0 30.0 25-29 30.0 30.0 30.0 30.0 30.0 30.0 30-34 30.0 30.0 30.0 30.0 30.0 30.0 35-39 30.0 30.0 30.0 30.0 30.0 30.0 40-44 30.0 30.0 30.0 30.0 30.0 30.0 45-49 30.0 30.0 30.0 30.0 30.0 30.0 50-54 30.0 30.0 30.0 30.0 30.0 30.0 55-59 30.0 30.0 30.0 30.0 30.0 30.0 60-64 30.0 30.0 30.0 30.0 30.0 30.0 65-69 30.0 30.0 30.0 30.0 30.0 30.0 70-74 30.0 30.0 30.0 30.0 30.0 30.0 75-79 30.0 30.0 30.0 30.0 30.0 30.0 80-84 30.0 30.0 30.0 30.0 30.0 30.0 85-89 30.0 30.0 30.0 30.0 30.0 30.0 90-94 30.0 30.0 30.0 30.0 30.0 30.0 95-99 30.0 30.0 30.0 30.0 30.0 30.0 100-104 30.0 30.0 30.0 30.0 30.0 30.0 105-109 30.0 30.0 30.0 30.0 30.0 30.0 110-114 30.0 30.0 30.0 30.0 30.0 30.0 115-119 30.0 30.0 30.0 30.0 30.0 30.0 120-124 30.0 30.0 30.0 30.0 30.0 30.0 125-129 30.0 30.0 30.0 30.0 30.0 30.0 130-134 30.0 30.0 30.0 30.0 30.0 30.0 135-139 30.0 30.0 30.0 30.0 30.0 30.0 140-144 30.0 30.0 30.0 30.0 30.0 30.0 145-149 30.0 30.0 30.0 30.0 30.0 30.0 150-151 30.0 30.0 30.0 30.0 30.0 30.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 1.3419171E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.45114271283227 13.285580100316107 12.187109014498311 29.076168172353313 2 20.660713397970056 16.86656111268004 35.12759458623277 27.345130903117138 3 18.041159174437823 23.535522425342073 30.866303141975017 27.557015258245087 4 21.63917577322772 30.518703428102974 25.266642775473986 22.575478023195323 5 21.747923176476398 34.75035082271475 25.154161907617095 18.34756409319175 6 18.13711038768443 35.631043549776095 26.418407476287836 19.813438586251642 7 14.154384052487295 23.96941659063738 44.00560213443885 17.87059722243647 8 16.61500550220278 24.721191793442383 32.365404688560865 26.29839801579397 9 17.61236219435612 23.978433541088343 33.43664821023594 24.9725560543196 10-14 19.945126267487016 30.059628869771466 26.789870998737552 23.20537386400397 15-19 19.874118900489457 29.02056915438368 27.774210493330777 23.33110145179609 20-24 19.854932916496853 29.023013418638154 27.844553139683516 23.277500525181473 25-29 19.825662852049504 29.01846917369188 27.81118893260992 23.344679041648696 30-34 19.669142005866085 29.215743655103584 27.736170885667978 23.378943453362357 35-39 19.94294729532845 29.065668810688827 27.571111509049256 23.420272384933465 40-44 19.986110915495452 29.38842794387224 27.431661762116306 23.193799378516005 45-49 20.209063585224452 28.839744273323593 27.546479585065274 23.40471255638668 50-54 20.256506158241816 28.711332466066647 27.621600469954515 23.410560905737025 55-59 19.956459307359598 28.56782732703831 27.9344692753375 23.541244090264595 60-64 20.086488204077586 28.49509109020222 27.89580369756075 23.522617008159447 65-69 20.077036399803237 28.894332866014743 27.56467250326791 23.463958230914102 70-74 20.02004594769677 29.039752157566216 27.51346115195939 23.42674074277763 75-79 20.071991034319485 28.97746813122808 27.493522513425013 23.45701832102743 80-84 20.1388103631737 28.86073960902652 27.419602894992543 23.580847132807232 85-89 20.35913395842411 28.87655727764405 27.242202964698787 23.522105799233053 90-94 20.383542321653103 28.71070500554766 27.367246456580663 23.538506216218572 95-99 20.421365820586086 28.664965965483262 27.339357997599105 23.574310216331547 100-104 20.61702321253675 28.80400734143711 27.103087068493277 23.47588237753286 105-109 20.68284956661149 28.581315287336228 27.205183012713924 23.530652133338357 110-114 20.784100965769134 28.533754748374967 27.155636707123264 23.526507578732627 115-119 20.88147173920058 28.810949648081834 26.795348237234624 23.512230375482957 120-124 20.801910937717388 28.866324156685984 26.532010062320545 23.799754843276087 125-129 20.801773820454333 28.79366094969652 26.53235285547818 23.872212374370967 130-134 20.946384840017316 28.894901182792886 26.34690473800505 23.811809239184743 135-139 20.941737757123747 28.959793417939157 26.203407051001882 23.89506177393522 140-144 20.8940179687702 28.904754250467484 26.184632418798447 24.016595361963866 145-149 20.740023359118084 29.088984707028477 26.09486830445785 24.07612362939559 150-151 20.59544140245325 29.436859400629146 25.89911105536996 24.068588141547643 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10668.0 1 8393.5 2 5543.0 3 4761.0 4 4296.5 5 3863.0 6 3458.0 7 3010.0 8 2589.0 9 2257.5 10 2023.5 11 1864.0 12 1771.0 13 1697.0 14 1704.0 15 1796.5 16 1950.5 17 2321.0 18 2827.0 19 3415.0 20 4524.0 21 6053.5 22 8091.5 23 11042.5 24 15040.0 25 20004.5 26 27478.5 27 38299.5 28 51973.0 29 70192.5 30 92749.0 31 121353.0 32 155021.0 33 196054.5 34 249184.5 35 308283.5 36 364573.5 37 420605.5 38 477668.0 39 538622.0 40 601467.5 41 662967.0 42 714440.0 43 753435.0 44 784021.0 45 783960.5 46 766747.0 47 748032.5 48 710208.0 49 649041.0 50 570033.5 51 478252.5 52 400682.0 53 350469.5 54 296972.0 55 237026.0 56 185637.0 57 141838.0 58 103929.0 59 77059.5 60 55384.0 61 35810.5 62 24370.0 63 15959.0 64 9398.5 65 5916.0 66 4164.5 67 3092.0 68 2491.5 69 1759.5 70 1143.0 71 634.5 72 414.5 73 345.5 74 225.5 75 76.5 76 47.5 77 17.0 78 8.5 79 3.0 80 0.5 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1158119231061293 2 0.017437738888639247 3 0.0 4 0.0 5 0.0 6 0.002377196027981162 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 4.471215099651089E-4 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 7.750106172728554E-5 110-114 8.942430199302178E-6 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 1.3419171E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.24263375426447 #Duplication Level Percentage of deduplicated Percentage of total 1 68.80699212363174 28.37781575887504 2 15.311254024727866 12.629528841207183 3 5.8880942085725305 7.285215388642877 4 2.838324539318892 4.682399178034818 5 1.6680044575328814 3.439644847125461 6 1.0854611907033767 2.6860367005588306 7 0.7878292792837893 2.274450809847122 8 0.5806678001974099 1.9158615533148955 9 0.4177396893864269 1.5505816512586144 >10 2.3264807879054885 17.51298642859079 >50 0.17588008520731743 4.997431784632281 >100 0.10333954786901267 7.899353178172369 >500 0.006705922180380772 1.861816529430153 >1k 0.0031008794159446975 2.1217434674776934 >5k 1.0754062891056771E-4 0.295780015397511 >10k+ 1.7923438151761283E-5 0.46935386743432284 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 63494 0.47315888589541044 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0023399359021507365 0.0 0.0 0.0 0.0 2 0.002369744002815077 0.0 0.0 0.0 0.0 3 0.0023995521034794174 0.0 0.0 0.0 0.0 4 0.002466620329974184 0.0 0.0 0.0 0.0 5 0.0024815243803063543 7.452025166085148E-6 0.0 0.0 0.0 6 0.002578400707465461 7.452025166085148E-6 0.0 0.0 0.0 7 0.002623112858461972 7.452025166085148E-6 0.0 0.0 0.0 8 0.002667825009458483 7.452025166085148E-6 0.0 0.0 0.0 9 0.0026976331101228237 7.452025166085148E-6 0.0 0.0 0.0 10-11 0.0028690296889427817 1.863006291521287E-5 0.0 0.0 0.0 12-13 0.0032639870227452947 2.2356075498255445E-5 0.0 7.452025166085148E-6 0.0 14-15 0.003535985941307403 2.2356075498255445E-5 0.0 2.2356075498255445E-5 0.0 16-17 0.00364776631879868 2.9808100664340592E-5 0.0 4.098613841346831E-5 0.0 18-19 0.003707382520127361 3.726012583042574E-5 0.0 4.471215099651089E-5 0.0 20-21 0.003811710872452553 3.726012583042574E-5 0.0 4.471215099651089E-5 0.0 22-23 0.003934669287692958 3.726012583042574E-5 0.0 4.471215099651089E-5 0.0 24-25 0.004053901690350321 4.471215099651089E-5 0.0 4.471215099651089E-5 0.0 26-27 0.004176860105590725 5.216417616259604E-5 0.0 4.471215099651089E-5 0.0 28-29 0.0043519826969937265 5.216417616259604E-5 0.0 6.706822649476633E-5 0.0 30-31 0.004542009338728897 5.589018874563861E-5 0.0 7.452025166085148E-5 0.0 32-33 0.004758118068545367 5.9616201328681184E-5 0.0 7.452025166085148E-5 0.0 34-35 0.004977952810944879 6.334221391172376E-5 0.0 7.824626424389406E-5 0.0 36-37 0.005264855779839157 6.706822649476633E-5 0.0 8.569828940997921E-5 0.0 38-39 0.005711977289804266 6.706822649476633E-5 0.0 8.942430199302178E-5 0.0 40-41 0.006330495378589333 6.706822649476633E-5 0.0 1.0432835232519207E-4 0.0 42-43 0.006956465492540486 8.942430199302177E-5 0.0 1.1923240265736237E-4 0.0 44-45 0.007761284210477681 1.2295841524040494E-4 0.0 1.1923240265736237E-4 0.0 46-47 0.008711417419153537 1.4531449073866039E-4 0.0 1.2668442782344752E-4 0.0 48-49 0.010138480238458845 1.5276651590474555E-4 0.0 1.3413645298953266E-4 0.0 50-51 0.012243677347877898 1.6767056623691583E-4 0.0 1.3786246557257522E-4 0.0 52-53 0.014844434130841615 1.9747866690125642E-4 0.0 1.415884781556178E-4 0.0 54-55 0.017810340146943503 2.161087298164693E-4 0.0 1.415884781556178E-4 0.0 56-57 0.021543804755152163 2.161087298164693E-4 0.0 1.415884781556178E-4 0.0 58-59 0.026197594471372335 2.2356075498255443E-4 0.0 1.415884781556178E-4 0.0 60-61 0.032580254026124265 2.3846480531472474E-4 0.0 1.415884781556178E-4 0.0 62-63 0.040792385759150104 2.459168304808099E-4 0.0 1.415884781556178E-4 0.0 64-65 0.051076180488347604 2.459168304808099E-4 0.0 1.415884781556178E-4 0.0 66-67 0.06312983119449032 2.5336885564689504E-4 0.0 1.564925284877881E-4 0.0 68-69 0.07765755425577332 2.608208808129802E-4 0.0 1.6767056623691583E-4 0.0 70-71 0.09745385910947851 2.682729059790653E-4 0.0 1.713965788199584E-4 0.0 72-73 0.12321551010863488 2.757249311451505E-4 0.0 1.7512259140300097E-4 0.0 74-75 0.15633230994671726 2.9062898147732077E-4 0.0 1.9747866690125642E-4 0.0 76-77 0.1970650795045387 2.9062898147732077E-4 0.0 2.0865670465038415E-4 0.0 78-79 0.24483628683172753 2.9062898147732077E-4 0.0 2.1983474239951184E-4 0.0 80-81 0.30441150202199524 3.129850569755762E-4 0.0 2.459168304808099E-4 0.0 82-83 0.37969931227495346 3.278891073077465E-4 0.0 2.570948682299376E-4 0.0 84-85 0.47533860325649024 3.278891073077465E-4 0.0 2.682729059790653E-4 0.0 86-87 0.5915156755957578 3.427931576399168E-4 0.0 2.682729059790653E-4 0.0 88-89 0.7255068140945518 3.427931576399168E-4 0.0 2.682729059790653E-4 0.0 90-91 0.8785453289178593 3.427931576399168E-4 0.0 2.682729059790653E-4 0.0 92-93 1.0595624722272337 3.427931576399168E-4 0.0 2.831769563112356E-4 0.0 94-95 1.276461116711308 3.427931576399168E-4 0.0 2.831769563112356E-4 0.0 96-97 1.5281383626455018 3.4651917022295937E-4 0.0 2.9808100664340594E-4 0.0 98-99 1.799120079772439 3.5769720797208706E-4 0.0 3.129850569755762E-4 0.0 100-101 2.093355841430145 3.949573338025128E-4 0.0 3.2043708214166133E-4 0.0 102-103 2.4160695172600457 4.0240935896859796E-4 0.0 3.241630947247039E-4 0.0 104-105 2.7772691770601927 4.0240935896859796E-4 0.0 3.3534113247383167E-4 0.0 106-107 3.1750247463125705 4.2476543446685346E-4 0.0 3.427931576399168E-4 0.0 108-109 3.592710756871643 4.54573535131194E-4 0.0 3.427931576399168E-4 0.0 110-111 4.040461962963286 4.54573535131194E-4 0.0 3.427931576399168E-4 0.0 112-113 4.5068134238694775 4.54573535131194E-4 0.0 3.4651917022295937E-4 0.0 114-115 5.016036385556157 4.843816357955346E-4 0.0 3.5397119538904453E-4 0.0 116-117 5.556990070400027 5.067377112937901E-4 0.0 3.576972079720871E-4 0.0 118-119 6.118548604828122 5.216417616259603E-4 0.0 3.576972079720871E-4 0.0 120-121 6.695793652230828 5.216417616259603E-4 0.0 3.576972079720871E-4 0.0 122-123 7.291180654900366 5.365458119581306E-4 0.0 3.6142322055512965E-4 0.0 124-125 7.92149902553593 5.58901887456386E-4 0.0 3.688752457212148E-4 0.0 126-127 8.578406221964084 5.924360007037693E-4 0.0 3.726012583042574E-4 0.0 128-129 9.250142948472748 6.110660636189821E-4 0.0 3.726012583042574E-4 0.0 130-131 9.934637542065751 6.110660636189821E-4 0.0 3.763272708873E-4 0.0 132-133 10.625224166232027 6.185180887850673E-4 0.0 3.8005328347034256E-4 0.0 134-135 11.344739552093046 6.334221391172376E-4 0.0 4.0240935896859796E-4 0.0 136-137 12.093723971473349 6.334221391172376E-4 0.0 4.098613841346831E-4 0.0 138-139 12.859527611653506 6.818603026967911E-4 0.0 4.098613841346831E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCCGAT 3660 0.0 33.12815 1 CGATTCA 6990 0.0 26.863695 4 GTCGGTT 5415 0.0 24.80476 1 CCGATTC 5845 0.0 22.69916 3 CGGTTCG 4850 0.0 21.974487 3 TCGGTCC 4445 0.0 19.89848 7 TCGGTTC 5465 0.0 19.506618 2 CCCGATT 6275 0.0 18.953306 2 GTTCGGT 4610 0.0 18.557705 5 GTCCGAT 2480 0.0 17.858294 1 GGTTCGG 5170 0.0 17.80969 4 TTCGCAC 5410 0.0 17.689213 8 TTCGGTC 5225 0.0 16.928432 6 TTTTTTA 45790 0.0 16.625008 4 CGGTCCT 5540 0.0 15.834613 8 ACGTCGG 2050 0.0 15.561161 5 TATCGGA 3695 0.0 15.129971 1 TTTTTAA 29010 0.0 14.995009 5 TCGCACT 4325 0.0 14.58361 9 TCGACGT 2445 0.0 13.940356 2 >>END_MODULE SRR7171060 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7171060_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13419171 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0 30.0 30.0 30.0 30.0 30.0 2 30.0 30.0 30.0 30.0 30.0 30.0 3 30.0 30.0 30.0 30.0 30.0 30.0 4 30.0 30.0 30.0 30.0 30.0 30.0 5 30.0 30.0 30.0 30.0 30.0 30.0 6 30.0 30.0 30.0 30.0 30.0 30.0 7 30.0 30.0 30.0 30.0 30.0 30.0 8 30.0 30.0 30.0 30.0 30.0 30.0 9 30.0 30.0 30.0 30.0 30.0 30.0 10-14 30.0 30.0 30.0 30.0 30.0 30.0 15-19 30.0 30.0 30.0 30.0 30.0 30.0 20-24 30.0 30.0 30.0 30.0 30.0 30.0 25-29 30.0 30.0 30.0 30.0 30.0 30.0 30-34 30.0 30.0 30.0 30.0 30.0 30.0 35-39 30.0 30.0 30.0 30.0 30.0 30.0 40-44 30.0 30.0 30.0 30.0 30.0 30.0 45-49 30.0 30.0 30.0 30.0 30.0 30.0 50-54 30.0 30.0 30.0 30.0 30.0 30.0 55-59 30.0 30.0 30.0 30.0 30.0 30.0 60-64 30.0 30.0 30.0 30.0 30.0 30.0 65-69 30.0 30.0 30.0 30.0 30.0 30.0 70-74 30.0 30.0 30.0 30.0 30.0 30.0 75-79 30.0 30.0 30.0 30.0 30.0 30.0 80-84 30.0 30.0 30.0 30.0 30.0 30.0 85-89 30.0 30.0 30.0 30.0 30.0 30.0 90-94 30.0 30.0 30.0 30.0 30.0 30.0 95-99 30.0 30.0 30.0 30.0 30.0 30.0 100-104 30.0 30.0 30.0 30.0 30.0 30.0 105-109 30.0 30.0 30.0 30.0 30.0 30.0 110-114 30.0 30.0 30.0 30.0 30.0 30.0 115-119 30.0 30.0 30.0 30.0 30.0 30.0 120-124 30.0 30.0 30.0 30.0 30.0 30.0 125-129 30.0 30.0 30.0 30.0 30.0 30.0 130-134 30.0 30.0 30.0 30.0 30.0 30.0 135-139 30.0 30.0 30.0 30.0 30.0 30.0 140-144 30.0 30.0 30.0 30.0 30.0 30.0 145-149 30.0 30.0 30.0 30.0 30.0 30.0 150-151 30.0 30.0 30.0 30.0 30.0 30.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 1.3419171E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.86821033877577 21.187992909547095 10.911657657540843 20.03213909413629 2 26.670740179487947 24.519022172101966 30.132614976129034 18.67762267228105 3 22.027816360687098 26.779533289801176 32.15784345624832 19.0348068932634 4 25.18728489962579 33.76039875405754 22.210753349185868 18.841562997130804 5 25.004937870778193 37.0580680187414 20.935856574120905 17.001137536359497 6 20.968515153333016 37.45542046926763 22.904576684021418 18.67148769337793 7 20.26120295005404 20.694278263948878 38.6450208746549 20.399497911342184 8 20.886842329412396 25.090458337894596 27.820897297528514 26.201802035164494 9 22.728718100167995 24.988097098464216 28.322280718894266 23.960904082473526 10-14 24.21412766347113 28.4454989218431 25.855305170166183 21.48506824451959 15-19 23.91460614613147 27.89284807828371 27.402005445821548 20.790540329763274 20-24 23.83990018204936 28.225827113727213 27.299170351371522 20.635102352851906 25-29 23.82443922933893 28.291664211000057 27.27911722280696 20.60477933685405 30-34 23.685280269709946 27.92916685918178 27.71015421578286 20.675398655325413 35-39 23.517451640068117 27.84815031990149 27.720068101649165 20.914329938381236 40-44 23.81938229288044 27.864186659461453 27.700519169757076 20.61591187790103 45-49 23.591542357917923 27.820564607297804 27.73987111994679 20.84802191483748 50-54 23.583878428334845 27.605748812848436 27.90222798923144 20.908144769585274 55-59 23.90815697966556 27.383485729739192 27.8033558728341 20.90500141776115 60-64 23.571960134851242 27.386894258567825 27.94866960417294 21.092476002407995 65-69 23.545963510742496 27.49786790213524 28.03498604988392 20.92118253723834 70-74 23.61611627725341 28.00126336474753 27.531365012249093 20.851255345749962 75-79 23.4686905447242 27.981219287154346 27.648600629388277 20.90148953873318 80-84 23.57771356811034 28.033233063941697 27.62380948604926 20.765243881898705 85-89 23.782581392718384 27.97134937323009 27.598449995088252 20.647619238963273 90-94 23.86431836849514 27.913719905581623 27.650878447663708 20.571083278259525 95-99 23.746460228166985 28.01089116997582 27.70155982467771 20.541088777179485 100-104 24.158916028243997 27.856397023868123 27.562043549743088 20.422643398144796 105-109 24.178625788417392 27.87033790259118 27.662610307261343 20.28842600173008 110-114 24.358489842176414 28.093790808086215 27.433844304565003 20.113875045172364 115-119 24.723616178955005 28.226232039245662 27.18055121702458 19.869600564774757 120-124 24.885149322909818 28.172631222531948 27.125301142575104 19.816918311983134 125-129 25.05670557394638 28.166922035948723 27.109931836754424 19.666440553350473 130-134 25.465725844374532 28.045147260068067 26.991815127459734 19.49731176809767 135-139 25.630872468215987 28.016679199666715 27.050971425505168 19.301476906612134 140-144 25.85688545027109 28.111028936262134 26.911561164564514 19.12052444890227 145-149 26.300014172243497 28.143319540089536 26.66882191690653 18.887844370760437 150-151 26.583467006485247 28.217159720320367 26.55156430190943 18.647808971284956 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 469.0 1 349.5 2 244.0 3 266.0 4 262.5 5 273.5 6 318.5 7 336.5 8 353.5 9 402.0 10 450.0 11 527.0 12 607.0 13 676.0 14 746.5 15 861.0 16 1015.5 17 1318.0 18 1670.5 19 2082.0 20 2736.0 21 3550.5 22 4254.5 23 5322.5 24 7205.5 25 9711.0 26 13507.5 27 18593.5 28 25681.0 29 36423.5 30 50903.0 31 69340.5 32 91902.0 33 120616.5 34 160876.5 35 213389.0 36 273288.5 37 346101.0 38 428925.5 39 514369.0 40 603074.5 41 691506.0 42 769955.5 43 821191.5 44 851483.5 45 861029.0 46 844153.0 47 816951.5 48 775904.5 49 705506.5 50 611449.5 51 512834.5 52 434338.0 53 380156.0 54 334824.0 55 272765.0 56 199327.5 57 145914.5 58 107428.0 59 80679.5 60 58997.5 61 41208.0 62 32585.0 63 22231.5 64 11657.5 65 6243.5 66 3925.5 67 2947.0 68 2502.5 69 2021.5 70 1436.5 71 954.5 72 677.5 73 488.0 74 325.5 75 161.5 76 118.0 77 84.5 78 72.0 79 60.5 80 40.0 81 30.5 82 32.0 83 30.0 84 24.0 85 20.0 86 16.5 87 12.5 88 8.0 89 7.5 90 8.5 91 7.0 92 7.0 93 8.0 94 9.5 95 7.5 96 7.0 97 7.5 98 3.5 99 3.5 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0057902235540481595 3 0.005492142547404754 4 0.016237962836899537 5 0.018309625833071205 6 0.001311556429230986 7 2.0120467948429898E-4 8 7.452025166085148E-5 9 0.0012966523788988157 10-14 0.0011878528114739727 15-19 0.0027512876913186367 20-24 0.0037438974434411782 25-29 0.009134692448587174 30-34 0.002317579826652481 35-39 0.0056545966960254105 40-44 0.011020054815606715 45-49 0.001819784545557993 50-54 0.004644102083504264 55-59 0.006198594533149627 60-64 0.003971929413523384 65-69 0.006940816239691706 70-74 0.0077128460468981275 75-79 0.00507780994817042 80-84 0.008827669011744466 85-89 0.00240849453367872 90-94 0.0038124560749691616 95-99 0.0016916097127013287 100-104 0.00606594848519331 105-109 0.009762152967571543 110-114 0.004234240699369581 115-119 0.00961907408438271 120-124 0.010113888555410762 125-129 0.006149411167053464 130-134 0.00455616818654446 135-139 0.009397003734433371 140-144 0.008717379039286407 145-149 0.010389613486555913 150-151 0.0018331981908569464 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 1.3419171E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.160526322990336 #Duplication Level Percentage of deduplicated Percentage of total 1 67.75222864563364 28.564696192554965 2 16.110283701973756 13.584360801758136 3 6.193288107431981 7.83336858837747 4 3.0096298779747523 5.075503187712509 5 1.7323936104980884 3.651931320859246 6 1.1028313432005772 2.7897569924896035 7 0.7351855645286259 2.169706724191415 8 0.5245023019659844 1.7690634486804726 9 0.39848276154503137 1.5120218661639457 >10 2.1718112373777654 16.734907729254278 >50 0.1669316598817708 4.821130291978058 >100 0.09482914831273222 7.26427562206666 >500 0.005037225447154626 1.4307719337255154 >1k 0.002424281062186016 1.8593040886746441 >5k 8.783322877829128E-5 0.23802755030497982 >10k+ 5.269993726697477E-5 0.7011736612083034 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 68531 0.5106947366569813 Illumina Single End PCR Primer 1 (97% over 34bp) AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 14827 0.1104911771375445 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002421908178977673 7.452025166085148E-6 0.0 5.216417616259604E-5 0.0 2 0.0024442642544759284 7.452025166085148E-6 7.452025166085148E-6 5.216417616259604E-5 0.0 3 0.0024517162796420136 7.452025166085148E-6 7.452025166085148E-6 5.9616201328681184E-5 0.0 4 0.002511332480970695 7.452025166085148E-6 2.2356075498255445E-5 5.9616201328681184E-5 0.0 5 0.0025262365313028653 7.452025166085148E-6 1.0432835232519207E-4 6.706822649476633E-5 0.0 6 0.002667825009458483 7.452025166085148E-6 1.0432835232519207E-4 7.452025166085148E-5 0.0 7 0.002705085135288909 7.452025166085148E-6 1.0432835232519207E-4 7.452025166085148E-5 0.0 8 0.002727441210787164 7.452025166085148E-6 1.0432835232519207E-4 7.452025166085148E-5 0.0 9 0.0027497972862854194 7.452025166085148E-6 1.0432835232519207E-4 7.452025166085148E-5 0.0 10-11 0.002842947600861484 7.452025166085148E-6 1.0432835232519207E-4 7.824626424389406E-5 0.0 12-13 0.0031186725320066343 7.452025166085148E-6 1.0432835232519207E-4 8.197227682693663E-5 0.0 14-15 0.0033645893624874444 7.452025166085148E-6 1.0432835232519207E-4 8.197227682693663E-5 0.0 16-17 0.0034689177148126363 7.452025166085148E-6 1.0432835232519207E-4 8.197227682693663E-5 0.0 18-19 0.0035546160042226153 7.452025166085148E-6 1.0432835232519207E-4 8.197227682693663E-5 0.0 20-21 0.0036775744194630206 7.452025166085148E-6 1.0432835232519207E-4 8.197227682693663E-5 0.0 22-23 0.003804258847286468 7.452025166085148E-6 1.0432835232519207E-4 8.942430199302177E-5 0.0 24-25 0.003938395300276001 7.452025166085148E-6 1.0432835232519207E-4 1.0805436490823464E-4 0.0 26-27 0.004094887828763789 7.452025166085148E-6 1.0432835232519207E-4 1.2295841524040494E-4 0.0 28-29 0.004303544533414173 7.452025166085148E-6 1.1178037749127721E-4 1.2668442782344752E-4 0.0 30-31 0.00453083130097977 7.452025166085148E-6 1.1178037749127721E-4 1.2668442782344752E-4 0.0 32-33 0.004784200156626665 7.452025166085148E-6 1.1178037749127721E-4 1.2668442782344752E-4 0.0 34-35 0.00502266496194139 7.452025166085148E-6 1.155063900743198E-4 1.2668442782344752E-4 0.0 36-37 0.005302115905669582 7.452025166085148E-6 1.7139657881995842E-4 1.3413645298953266E-4 0.0 38-39 0.005752963428217734 7.452025166085148E-6 1.863006291521287E-4 1.415884781556178E-4 0.0 40-41 0.006352851454087588 7.452025166085148E-6 1.863006291521287E-4 1.415884781556178E-4 1.1178037749127722E-5 42-43 0.007023533719035252 7.452025166085148E-6 1.863006291521287E-4 1.415884781556178E-4 1.4904050332170296E-5 44-45 0.007854434525053746 1.4904050332170296E-5 2.1238271723342673E-4 1.415884781556178E-4 1.4904050332170296E-5 46-47 0.008826923809227859 1.4904050332170296E-5 2.27286767565597E-4 1.415884781556178E-4 1.4904050332170296E-5 48-49 0.010425383207353122 1.4904050332170296E-5 2.6082088081298016E-4 1.415884781556178E-4 1.4904050332170296E-5 50-51 0.012500772216107837 1.4904050332170296E-5 2.6082088081298016E-4 1.415884781556178E-4 1.4904050332170296E-5 52-53 0.015198405326230659 1.4904050332170296E-5 2.6082088081298016E-4 1.6021854107083067E-4 1.4904050332170296E-5 54-55 0.018127051216502123 1.4904050332170296E-5 2.869029688942782E-4 1.8257461656908611E-4 1.4904050332170296E-5 56-57 0.02174500943463646 1.4904050332170296E-5 2.9808100664340594E-4 1.863006291521287E-4 1.4904050332170296E-5 58-59 0.026409977188605764 1.4904050332170296E-5 2.9808100664340594E-4 2.0120467948429898E-4 1.4904050332170296E-5 60-61 0.03276282864269335 1.4904050332170296E-5 2.9808100664340594E-4 2.2356075498255443E-4 1.4904050332170296E-5 62-63 0.04090416613664138 1.4904050332170296E-5 2.9808100664340594E-4 2.310127801486396E-4 1.4904050332170296E-5 64-65 0.051176782828089754 1.863006291521287E-5 2.9808100664340594E-4 2.4219081789776732E-4 1.4904050332170296E-5 66-67 0.06348007637729633 2.2356075498255445E-5 2.9808100664340594E-4 2.6082088081298016E-4 1.4904050332170296E-5 68-69 0.0779966214008302 2.608208808129802E-5 3.129850569755762E-4 2.7572493114515044E-4 1.4904050332170296E-5 70-71 0.09777802220420323 2.9808100664340592E-5 3.1671106955861875E-4 2.869029688942782E-4 1.4904050332170296E-5 72-73 0.12353594719077654 2.9808100664340592E-5 3.2043708214166133E-4 2.9808100664340594E-4 1.4904050332170296E-5 74-75 0.1567198152553537 2.9808100664340592E-5 3.278891073077465E-4 2.9808100664340594E-4 1.4904050332170296E-5 76-77 0.19739296861184646 2.9808100664340592E-5 3.3906714505687425E-4 3.0925904439253364E-4 1.4904050332170296E-5 78-79 0.24480647873106318 2.9808100664340592E-5 3.576972079720871E-4 3.2043708214166133E-4 1.4904050332170296E-5 80-81 0.3041581331663483 2.9808100664340592E-5 3.576972079720871E-4 3.2043708214166133E-4 1.4904050332170296E-5 82-83 0.37917021848816146 2.9808100664340592E-5 3.576972079720871E-4 3.278891073077465E-4 1.4904050332170296E-5 84-85 0.47445181226172617 2.9808100664340592E-5 3.6142322055512965E-4 3.278891073077465E-4 1.4904050332170296E-5 86-87 0.5903606116950145 2.9808100664340592E-5 3.726012583042574E-4 3.316151198907891E-4 1.4904050332170296E-5 88-89 0.7241617235520734 2.9808100664340592E-5 3.8005328347034256E-4 3.3534113247383167E-4 1.4904050332170296E-5 90-91 0.876928239456819 2.9808100664340592E-5 3.8377929605338515E-4 3.3534113247383167E-4 1.4904050332170296E-5 92-93 1.0578261503635358 2.9808100664340592E-5 3.875053086364277E-4 3.3534113247383167E-4 1.4904050332170296E-5 94-95 1.2745533982687902 2.9808100664340592E-5 3.875053086364277E-4 3.3534113247383167E-4 1.4904050332170296E-5 96-97 1.5267373819142778 2.9808100664340592E-5 3.875053086364277E-4 3.3534113247383167E-4 1.4904050332170296E-5 98-99 1.7980283580856073 2.9808100664340592E-5 3.9123132121947026E-4 3.3534113247383167E-4 1.863006291521287E-5 100-101 2.0930316783354206 2.9808100664340592E-5 3.9495733380251285E-4 3.5024518280600195E-4 2.2356075498255445E-5 102-103 2.417068088632301 2.9808100664340592E-5 3.9495733380251285E-4 3.6514923313817223E-4 2.2356075498255445E-5 104-105 2.7796277430252587 2.9808100664340592E-5 3.9495733380251285E-4 3.6514923313817223E-4 2.2356075498255445E-5 106-107 3.17955557761355 2.9808100664340592E-5 4.0240935896859796E-4 3.726012583042574E-4 2.2356075498255445E-5 108-109 3.599145580602557 2.9808100664340592E-5 4.0613537155164054E-4 3.875053086364277E-4 2.2356075498255445E-5 110-111 4.0481450009095195 2.9808100664340592E-5 4.135873967177257E-4 3.875053086364277E-4 2.2356075498255445E-5 112-113 4.516117277289335 2.9808100664340592E-5 4.173134093007683E-4 3.875053086364277E-4 2.2356075498255445E-5 114-115 5.029002909345145 3.726012583042574E-5 4.210394218838109E-4 3.875053086364277E-4 2.2356075498255445E-5 116-117 5.573291375450838 3.726012583042574E-5 4.247654344668534E-4 3.9123132121947026E-4 2.2356075498255445E-5 118-119 6.138322553606329 3.726012583042574E-5 4.4712150996510886E-4 4.0240935896859796E-4 2.2356075498255445E-5 120-121 6.71686052737535 3.726012583042574E-5 4.4712150996510886E-4 4.098613841346831E-4 2.2356075498255445E-5 122-123 7.3140285640595835 3.726012583042574E-5 4.582995477142366E-4 4.322174596329386E-4 2.2356075498255445E-5 124-125 7.948710095429889 3.726012583042574E-5 4.620255602972792E-4 4.322174596329386E-4 2.2356075498255445E-5 126-127 8.611727952494235 3.726012583042574E-5 4.620255602972792E-4 4.322174596329386E-4 2.2356075498255445E-5 128-129 9.291017306508724 3.726012583042574E-5 4.694775854633643E-4 4.322174596329386E-4 2.2356075498255445E-5 130-131 9.985076574402399 3.726012583042574E-5 4.694775854633643E-4 4.322174596329386E-4 2.2356075498255445E-5 132-133 10.686364306707173 3.726012583042574E-5 4.694775854633643E-4 4.3966948479902374E-4 2.2356075498255445E-5 134-135 11.416618060832521 3.726012583042574E-5 4.694775854633643E-4 4.4712150996510886E-4 2.2356075498255445E-5 136-137 12.179016125511776 3.726012583042574E-5 4.7692961062944947E-4 4.54573535131194E-4 2.608208808129802E-5 138-139 12.960912414038095 3.726012583042574E-5 4.918336609616198E-4 4.918336609616198E-4 2.9808100664340592E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTCGG 5020 0.0 25.70792 145 GAACACA 14175 0.0 20.715893 2 AGAACAC 16415 0.0 18.198183 1 CGGTAAT 4175 0.0 18.060406 145 CACATTC 14625 0.0 17.598606 5 TTCATAC 15690 0.0 16.35553 9 CCGCTCG 2350 0.0 16.043798 3 ATTCATA 16505 0.0 15.503469 8 ACACATT 17515 0.0 15.192388 4 GCGACTT 3975 0.0 14.957135 1 CGACTTA 3320 0.0 14.632159 2 ACATTCA 18470 0.0 14.364014 6 AACACAT 19830 0.0 14.223241 3 CATACTC 14185 0.0 13.133731 9 AGTCGAC 5170 0.0 12.901586 5 CGGGAAC 4455 0.0 12.857338 1 CATTCAT 20220 0.0 12.726543 7 GCCGTAT 17470 0.0 12.68235 55-59 CGACCCC 4925 0.0 12.657908 8 CGCCGTA 17675 0.0 12.609091 55-59 >>END_MODULE skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7171060 SRR7171060_1.fastq SRR7171060_2.fastq Input file: SRR7171060_1.fastq Paired file: SRR7171060_2.fastq trimmed: SRR7171060-trimmed-pair1.fastq, SRR7171060-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Apr 15 04:03:35 2025 >> started Tue Apr 15 04:03:50 2025 >> done (15.548s) 13419171 read pairs processed; of these: 228 ( 0.00%) short read pairs filtered out after trimming by size control 73009 ( 0.54%) empty read pairs filtered out after trimming by size control 13345934 (99.45%) read pairs available; of these: 2555070 (19.14%) trimmed read pairs available after processing 10790864 (80.86%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 9 0.00% 19 10 0.00% 20 12 0.00% 21 14 0.00% 22 8 0.00% 23 11 0.00% 24 9 0.00% 25 11 0.00% 26 9 0.00% 27 15 0.00% 28 17 0.00% 29 15 0.00% 30 14 0.00% 31 71 0.00% 32 15 0.00% 33 21 0.00% 34 18 0.00% 35 18 0.00% 36 29 0.00% 37 34 0.00% 38 36 0.00% 39 39 0.00% 40 52 0.00% 41 39 0.00% 42 54 0.00% 43 62 0.00% 44 52 0.00% 45 79 0.00% 46 68 0.00% 47 105 0.00% 48 118 0.00% 49 158 0.00% 50 154 0.00% 51 208 0.00% 52 195 0.00% 53 217 0.00% 54 232 0.00% 55 256 0.00% 56 277 0.00% 57 325 0.00% 58 388 0.00% 59 429 0.00% 60 533 0.00% 61 551 0.00% 62 625 0.00% 63 727 0.01% 64 785 0.01% 65 844 0.01% 66 933 0.01% 67 995 0.01% 68 1111 0.01% 69 1400 0.01% 70 1585 0.01% 71 1769 0.01% 72 2043 0.02% 73 2322 0.02% 74 2515 0.02% 75 2925 0.02% 76 3007 0.02% 77 3260 0.02% 78 3792 0.03% 79 4086 0.03% 80 4641 0.03% 81 5186 0.04% 82 5922 0.04% 83 6467 0.05% 84 7733 0.06% 85 8057 0.06% 86 8663 0.06% 87 9406 0.07% 88 10036 0.08% 89 10578 0.08% 90 11615 0.09% 91 12673 0.09% 92 13595 0.10% 93 15226 0.11% 94 16628 0.12% 95 17677 0.13% 96 18556 0.14% 97 19003 0.14% 98 19502 0.15% 99 20633 0.15% 100 21881 0.16% 101 22260 0.17% 102 24111 0.18% 103 25333 0.19% 104 26632 0.20% 105 28192 0.21% 106 28811 0.22% 107 29403 0.22% 108 30124 0.23% 109 31881 0.24% 110 32238 0.24% 111 32377 0.24% 112 34292 0.26% 113 36434 0.27% 114 36714 0.28% 115 38358 0.29% 116 39516 0.30% 117 39636 0.30% 118 40247 0.30% 119 40818 0.31% 120 41466 0.31% 121 42168 0.32% 122 42995 0.32% 123 44716 0.34% 124 46199 0.35% 125 46456 0.35% 126 47158 0.35% 127 47686 0.36% 128 48213 0.36% 129 49061 0.37% 130 49215 0.37% 131 49105 0.37% 132 50597 0.38% 133 51582 0.39% 134 52432 0.39% 135 54269 0.41% 136 54269 0.41% 137 54518 0.41% 138 55029 0.41% 139 56135 0.42% 140 55892 0.42% 141 57056 0.43% 142 57325 0.43% 143 57383 0.43% 144 58889 0.44% 145 59128 0.44% 146 60327 0.45% 147 60112 0.45% 148 61403 0.46% 149 60959 0.46% 150 62561 0.47% 151 10790864 80.86% 13345934 reads passed initial QC criterion=sequence-density sequence-density=0.56 sequence-density-rank=1 fanout-score=1.92 fanout-score-rank=39 prefix-density=0.53 prefix-fanout=1.9 sequence=GTTAGGGTAAGCTTTCTTCGCCTCCTCGAGCTCAATCAGCACCTGAGATGCCTCAGTGCATCCAAACATGGGTAGTTTCCACATAGTCCAGTAGCGTCCATCATAGTACCCTGGGGACTGGTGGTGCTCGCGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTGTTGCGAAGAAGGTACTCAATTTCCTGGGCCAATTGCTCAGTAGTGAGATCTGGAAGGTAAGAAAGAGTCTCGAACTTCTTCAATCCAGTTGGAGGCCACACCTGCATGCATTGAACTCTTCCGCCATTGCTTGCAATGGAAGTAATGTCATTGTTAGCCTTTCTGGTGACCGGGAAAGCTGAGGTAGACTTGAGGCCGTTGAATGGTGCAACCATGTTGGCCTGTGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=120.17 fanout-score-rank=1 prefix-density=0.10 prefix-fanout=10.5 sequence=AAACAGAATATTTACTTTTAGCGCAAGTTTCAATTCATGAGCTCGTTACATCACAAGTTAACATTACAAGCCAATAGTCCCAGCACAGAAAAAACTCTTTTGTTGCTCACTTTCCGGGAACGAAGTTTGTGGCATATGCCCAGGCGTTGTTGTTTACTGGGTCAGAAAGGTGGTCAGCCAGGTTCTCCAGTGGTCCCTTTCCGGTCACAATGGCCTGGACAAAGAATCCGAACATTGAGAA criterion=sequence-density sequence-density=0.84 sequence-density-rank=1 fanout-score=2.16 fanout-score-rank=32 prefix-density=0.85 prefix-fanout=2.1 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCATT criterion=fanout-score sequence-density=0.02 sequence-density-rank=33 fanout-score=62.29 fanout-score-rank=1 prefix-density=0.12 prefix-fanout=8.6 sequence=CTGAGCTTGAGTGCATTCTTCTAAGTAAAAGAAATCCCTTAATTTCATCACACTTTCCTTCTTTTCCAACAGAAAATGTCTTGCTGTGGAGGAAACTGTGGCTGCGGCTCTGGATGCAAGTGCGGCAGTGGCTGCAATGGATGCAGCATGTACCCAGACTTGAGTTTCTCCGAGACCACCACAAGTCAGACAATCATTGCTGGTGT SRR7171060 testing PE reads STAR mapping to Ensembl genome Started job on | Apr 15 04:04:42 Started mapping on | Apr 15 04:04:42 Finished on | Apr 15 04:07:26 Mapping speed, Million of reads per hour | 292.96 Number of input reads | 13345934 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 11989349 Uniquely mapped reads % | 89.84% Average mapped length | 290.60 Number of splices: Total | 10184135 Number of splices: Annotated (sjdb) | 9944875 Number of splices: GT/AG | 9971971 Number of splices: GC/AG | 167160 Number of splices: AT/AC | 7841 Number of splices: Non-canonical | 37163 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 2.78 Insertion rate per base | 0.02% Insertion average length | 2.00 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 394327 % of reads mapped to multiple loci | 2.95% Number of reads mapped to too many loci | 57054 % of reads mapped to too many loci | 0.43% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.66% % of reads unmapped: other | 0.13% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 962258 962258 962258 N_multimapping 394327 394327 394327 N_noFeature 413529 11733264 496883 N_ambiguous 265929 1311 92330 UnstrandedReadsAssigned:11309891 PositiveStrandReadsAssigned:254774 NegativeStrandReadsAssigned:11400136 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR7171060 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR7171060-trimmed-pair1.fastq SRR7171060-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 13,345,934 reads, 11,508,305 reads pseudoaligned [quant] estimated average fragment length: 210.903 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,098 rounds 52401 SRR7171060.ke.tsv 34699 SRR7171060.se.tsv 87100 total ==> SRR7171060.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1808.1 467 18.6143 Potri.005G024800.1.v4.1 1035 825.097 263 22.9721 Potri.004G059700.1.v4.1 961 751.097 28 2.68666 Potri.007G009000.2.v4.1 1416 1206.1 0 0 Potri.003G141000.2.v4.1 2943 2733.1 378 9.96753 Potri.016G087400.1.v4.1 270 92.9462 710 550.525 Potri.015G069301.1.v4.1 564 355.608 0 0 Potri.010G195200.1.v4.1 1773 1563.1 32 1.47542 Potri.012G127500.1.v4.1 977 767.097 36 3.38223 ==> SRR7171060.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 370 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 399 Potri.001G212900.v4.1 51 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 14 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1 SRR7171060 completed mapping pipeline successfully