Starting /dee2/code/volunteer_pipeline.sh SRR7171423 current disk space = 2796564570112 free memory = 1561180288 SRR7171423_1.fastq is conventional basespace SRR7171423_1.fastq read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7171423_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15823439 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0 30.0 30.0 30.0 30.0 30.0 2 30.0 30.0 30.0 30.0 30.0 30.0 3 30.0 30.0 30.0 30.0 30.0 30.0 4 30.0 30.0 30.0 30.0 30.0 30.0 5 30.0 30.0 30.0 30.0 30.0 30.0 6 30.0 30.0 30.0 30.0 30.0 30.0 7 30.0 30.0 30.0 30.0 30.0 30.0 8 30.0 30.0 30.0 30.0 30.0 30.0 9 30.0 30.0 30.0 30.0 30.0 30.0 10-14 30.0 30.0 30.0 30.0 30.0 30.0 15-19 30.0 30.0 30.0 30.0 30.0 30.0 20-24 30.0 30.0 30.0 30.0 30.0 30.0 25-29 30.0 30.0 30.0 30.0 30.0 30.0 30-34 30.0 30.0 30.0 30.0 30.0 30.0 35-39 30.0 30.0 30.0 30.0 30.0 30.0 40-44 30.0 30.0 30.0 30.0 30.0 30.0 45-49 30.0 30.0 30.0 30.0 30.0 30.0 50-54 30.0 30.0 30.0 30.0 30.0 30.0 55-59 30.0 30.0 30.0 30.0 30.0 30.0 60-64 30.0 30.0 30.0 30.0 30.0 30.0 65-69 30.0 30.0 30.0 30.0 30.0 30.0 70-74 30.0 30.0 30.0 30.0 30.0 30.0 75-79 30.0 30.0 30.0 30.0 30.0 30.0 80-84 30.0 30.0 30.0 30.0 30.0 30.0 85-89 30.0 30.0 30.0 30.0 30.0 30.0 90-94 30.0 30.0 30.0 30.0 30.0 30.0 95-99 30.0 30.0 30.0 30.0 30.0 30.0 100-104 30.0 30.0 30.0 30.0 30.0 30.0 105-109 30.0 30.0 30.0 30.0 30.0 30.0 110-114 30.0 30.0 30.0 30.0 30.0 30.0 115-119 30.0 30.0 30.0 30.0 30.0 30.0 120-124 30.0 30.0 30.0 30.0 30.0 30.0 125-129 30.0 30.0 30.0 30.0 30.0 30.0 130-134 30.0 30.0 30.0 30.0 30.0 30.0 135-139 30.0 30.0 30.0 30.0 30.0 30.0 140-144 30.0 30.0 30.0 30.0 30.0 30.0 145-149 30.0 30.0 30.0 30.0 30.0 30.0 150-151 30.0 30.0 30.0 30.0 30.0 30.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 1.5823439E7 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.8230106799073 10.641508755899212 10.071731060625472 38.46374950356802 2 22.74178336937644 14.261574298925822 33.64456443434927 29.352077897348465 3 20.313732052811023 19.809113556161844 25.003610150739043 34.8735442402881 4 22.890561274322224 27.52773907113365 23.096780668222628 26.484918986321492 5 22.580862478756988 31.414011833963528 24.86918298860317 21.13594269867631 6 19.606812400262676 34.96417561315211 25.061739107408954 20.367272879176266 7 14.63032783202185 26.012221489904945 41.2944114108191 18.0630392672541 8 18.1860529812767 25.592925785601977 30.99350273982792 25.2275184932934 9 17.461697169622862 24.688798686556062 33.83481302642238 24.01469111739869 10-14 19.959137833438103 29.611662799723877 27.066085950089615 23.36311341674841 15-19 19.977793702114948 28.385102631608717 27.730031379398625 23.907072286877714 20-24 20.020397588665777 28.542992455685518 27.692916817892748 23.743693137755958 25-29 19.984254784061324 28.697076113273788 27.564673155316882 23.75399594734801 30-34 19.98724757706429 28.642714481292153 27.5736638298357 23.79637411180786 35-39 20.084431678040666 28.48156907764126 27.539769379469035 23.89422986484904 40-44 20.20461460830221 28.601344047072264 27.4971638013347 23.696877543290825 45-49 20.213378857095503 28.389013936482225 27.462609219032064 23.934997987390208 50-54 20.18819285549231 28.470933143921773 27.489062157191118 23.8518118433948 55-59 20.194769291302606 28.47992019939534 27.44453465520359 23.880775854098466 60-64 20.193566013051903 28.395441724141 27.497299417655036 23.913692845152056 65-69 20.221918888807927 28.325242066531807 27.530158267112476 23.92268077754779 70-74 20.34029897040713 28.33718637269686 27.534188996462778 23.788325660433234 75-79 20.39549051252386 28.181791581463422 27.488229328656054 23.934488577356667 80-84 20.37742790757033 28.25394403430791 27.48302014775583 23.88560791036593 85-89 20.4249683121785 28.23726730486461 27.527393366793085 23.810371016163803 90-94 20.45464958660377 28.236017467505008 27.42547179535372 23.883861150537502 95-99 20.489164207603668 28.112574011250018 27.53222355772345 23.866038223422862 100-104 20.606018704277876 28.232731203374943 27.400269941319333 23.76098015102785 105-109 20.65345213515216 28.08533720135048 27.469401563086254 23.791809100411104 110-114 20.674589133247203 28.120366249081506 27.413518641554468 23.791525976116823 115-119 20.787751638566053 28.183379099827793 27.34865916315663 23.68021009844952 120-124 20.802002649360862 28.17149672710212 27.176142935805547 23.85035768773147 125-129 20.90243340907119 28.059853486969548 27.18177129510216 23.8559418088571 130-134 21.035305915483985 28.117982443639466 27.09084415846644 23.755867482410114 135-139 20.990302170388382 28.146976448221977 26.973331069684015 23.889390311705625 140-144 21.1124357985644 28.069626330913273 26.8917028719231 23.92623499859923 145-149 21.16745561892346 28.202033930285115 26.751513066077216 23.87899738471421 150-151 21.117305298463286 28.122423718690193 26.60455500281007 24.155715980036447 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 158.0 1 124.0 2 91.5 3 103.0 4 129.0 5 160.5 6 190.5 7 224.5 8 241.5 9 238.0 10 240.5 11 254.0 12 278.5 13 330.0 14 407.5 15 477.5 16 576.5 17 739.0 18 942.0 19 1230.5 20 1628.0 21 2189.5 22 3095.5 23 4402.0 24 6265.0 25 9009.0 26 13082.5 27 19281.5 28 27653.0 29 39675.0 30 56544.0 31 79421.5 32 108640.5 33 145734.0 34 193480.5 35 254744.5 36 329926.0 37 412778.0 38 505008.5 39 611036.5 40 724824.0 41 837763.5 42 940418.0 43 1021498.0 44 1071623.5 45 1088655.0 46 1071536.0 47 1023265.5 48 947192.0 49 842488.0 50 718332.5 51 600061.0 52 497513.0 53 399537.5 54 304859.5 55 225757.5 56 170125.0 57 128682.5 58 93721.5 59 67521.5 60 50193.5 61 38774.0 62 30759.5 63 22888.5 64 16395.0 65 12767.0 66 10379.5 67 9121.0 68 7404.0 69 5312.0 70 3671.5 71 2036.5 72 1796.5 73 2415.0 74 1820.5 75 733.5 76 504.5 77 197.0 78 119.5 79 88.0 80 27.5 81 2.5 82 2.0 83 1.5 84 0.5 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11471589709417783 2 0.0016178531101867298 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.001390342516566721 30-34 0.0055436747978742165 35-39 0.019124793289246416 40-44 0.012069436991541473 45-49 0.007562199342380629 50-54 7.444652202343625E-4 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 5.81415961473356E-5 85-89 6.193343937433576E-5 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 1.402981993990055E-4 140-144 0.0 145-149 3.66544845276681E-5 150-151 1.2639477423333826E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 1.5823439E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.045957942669794 #Duplication Level Percentage of deduplicated Percentage of total 1 68.95499135903059 32.44053623414112 2 15.47372250190547 14.559521960823762 3 5.787053216769744 8.16772386744424 4 2.884027389464462 5.4272732508101145 5 1.6639006052580358 3.9139898947876186 6 1.0988229278401236 3.1017106349764645 7 0.7720040788286456 2.54237699968994 8 0.5465093404856415 2.056884435821096 9 0.4282903642177093 1.8134397416013373 >10 2.2338714364848706 18.673090017337778 >50 0.11413934514680224 3.6131094219496167 >100 0.041521932219267886 3.269597911241387 >500 9.705945355881923E-4 0.3054415705613653 >1k 1.7490781290833567E-4 0.1153040588140873 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.84763419633368E-5 2.5278954846667656E-5 0.0 6.319738711666914E-6 0.0 2 8.84763419633368E-5 6.951712582833605E-5 0.0 6.319738711666914E-6 0.0 3 1.0743555809833754E-4 7.583686454000297E-5 0.0 6.319738711666914E-6 0.0 4 1.5799346779167285E-4 7.583686454000297E-5 0.0 1.895921613500074E-5 0.0 5 1.5799346779167285E-4 7.583686454000297E-5 0.0 2.5278954846667656E-5 0.0 6 2.0855137748500817E-4 7.583686454000297E-5 0.0 5.055790969333531E-5 0.0 7 2.21190854908342E-4 9.479608067500371E-5 0.0 5.687764840500223E-5 0.0 8 2.3383033233167583E-4 9.479608067500371E-5 0.0 9.479608067500371E-5 0.0 9 2.4015007104334274E-4 9.479608067500371E-5 0.0 1.7063294521500668E-4 0.0 10-11 2.6858889524584387E-4 9.479608067500371E-5 0.0 1.8959216135000742E-4 0.0 12-13 2.970277194483449E-4 9.795595003083717E-5 0.0 2.4015007104334271E-4 0.0 14-15 3.159869355833457E-4 1.0111581938667063E-4 0.0 2.591092871783435E-4 0.0 16-17 3.3178628236251297E-4 1.0743555809833754E-4 0.0 2.7490863395751076E-4 6.319738711666914E-6 18-19 3.349461517183464E-4 1.2639477423333827E-4 0.0 2.9070798073667803E-4 6.319738711666914E-6 20-21 3.570652372091806E-4 1.327145129450052E-4 0.0 2.9386785009251153E-4 6.319738711666914E-6 22-23 3.791843227000148E-4 1.3587438230083863E-4 0.0 2.97027719448345E-4 6.319738711666914E-6 24-25 4.0130340819084906E-4 1.548335984358394E-4 0.0 3.001875888041784E-4 6.319738711666914E-6 26-27 4.202626243258498E-4 1.6431320650333976E-4 0.0 3.1282706622751225E-4 6.319738711666914E-6 28-29 4.2974223239335016E-4 1.769526839266736E-4 0.0 3.254665436508461E-4 6.319738711666914E-6 30-31 4.3606197110501705E-4 1.769526839266736E-4 0.0 3.4126589043001335E-4 6.319738711666914E-6 32-33 4.392218404608505E-4 1.8011255328250706E-4 0.0 3.728645839883479E-4 6.319738711666914E-6 34-35 4.7398040337501854E-4 1.8959216135000742E-4 0.0 3.9182380012334867E-4 6.319738711666914E-6 36-37 4.8661988079835237E-4 2.0223163877334125E-4 0.0 4.1078301625834944E-4 6.319738711666914E-6 38-39 4.960994888658527E-4 2.0855137748500817E-4 0.0 4.1710275497001633E-4 6.319738711666914E-6 40-41 5.308580517800207E-4 2.1487111619667508E-4 0.0 4.1710275497001633E-4 6.319738711666914E-6 42-43 5.687764840500223E-4 2.3699020168750927E-4 0.0 4.234224936816832E-4 6.319738711666914E-6 44-45 6.288140018108579E-4 2.7490863395751076E-4 0.0 4.234224936816832E-4 6.319738711666914E-6 46-47 6.856916502158601E-4 2.875481113808446E-4 0.0 4.360619711050171E-4 6.319738711666914E-6 48-49 7.646883841116966E-4 2.9386785009251153E-4 0.0 4.487014485283509E-4 6.319738711666914E-6 50-51 8.816035502775345E-4 3.033474581600119E-4 0.0 4.6766066466335165E-4 6.319738711666914E-6 52-53 0.001058556234204208 3.286264130066795E-4 0.0 4.834600114425189E-4 6.319738711666914E-6 54-55 0.0012765872197567165 3.728645839883479E-4 0.0 5.055790969333531E-4 6.319738711666914E-6 56-57 0.0016336524569658972 3.855040614116818E-4 0.0 5.371777904916877E-4 6.319738711666914E-6 58-59 0.002101313121629249 4.1078301625834944E-4 0.0 5.529771372708549E-4 6.319738711666914E-6 60-61 0.002629011304053436 4.929396195100193E-4 0.0 5.592968759825219E-4 6.319738711666914E-6 62-63 0.0032673049139317943 5.371777904916877E-4 0.0 5.877357001850231E-4 6.319738711666914E-6 64-65 0.004268983499731 5.434975292033546E-4 0.0 6.509330873016922E-4 6.319738711666914E-6 66-67 0.005706724056635223 5.498172679150215E-4 0.0 7.078107357066944E-4 6.319738711666914E-6 68-69 0.007571046976576963 5.529771372708549E-4 0.0 7.33089690553362E-4 1.2639477423333828E-5 70-71 0.00991882990796122 5.592968759825219E-4 0.0 7.457291679766958E-4 1.895921613500074E-5 72-73 0.013461043455850526 5.750962227616891E-4 0.0 7.552087760441962E-4 2.5278954846667656E-5 74-75 0.018235606052514883 5.87735700185023E-4 0.0 7.773278615350303E-4 2.5278954846667656E-5 76-77 0.0246880592771268 6.035350469641902E-4 0.0 8.215660325166988E-4 2.5278954846667656E-5 78-79 0.03291635907971712 6.130146550316906E-4 0.0 8.78443680921701E-4 2.5278954846667656E-5 80-81 0.04327757069749502 6.509330873016922E-4 0.0 9.037226357683686E-4 2.5278954846667656E-5 82-83 0.057746612477856424 6.667324340808594E-4 0.0 9.669200228850379E-4 2.5278954846667656E-5 84-85 0.07675006678383883 6.951712582833605E-4 0.0 9.763996309525382E-4 2.5278954846667656E-5 86-87 0.1015013234480823 7.488890373325293E-4 0.0 9.921989777317056E-4 2.5278954846667656E-5 88-89 0.13107454074932762 7.583686454000297E-4 0.0 0.0010427568874250407 2.5278954846667656E-5 90-91 0.166566193354049 7.74167992179197E-4 0.0 0.0010680358422717085 2.5278954846667656E-5 92-93 0.2100491555596732 7.962870776700311E-4 0.0 0.0011122740132533768 2.5278954846667656E-5 94-95 0.26610523793215873 8.247259018725323E-4 0.0 0.0011154338826092104 2.5278954846667656E-5 96-97 0.33435209627944973 8.278857712283657E-4 0.0 0.0011185937519650438 2.5278954846667656E-5 98-99 0.4141830356852262 8.37365379295866E-4 0.0 0.0011249134906767106 2.5278954846667656E-5 100-101 0.5044257446184739 8.689640728542006E-4 0.0 0.0011501924455233784 2.5278954846667656E-5 102-103 0.6097599895951822 9.258417212592029E-4 0.0 0.0011628319229467121 2.5278954846667656E-5 104-105 0.7331781668953254 0.001030117410001707 0.0 0.0011691516616583791 2.5278954846667656E-5 106-107 0.8780771360764243 0.0010648759729158749 0.0 0.001181791139081713 2.5278954846667656E-5 108-109 1.0398908859193 0.0011122740132533768 0.0 0.001181791139081713 2.5278954846667656E-5 110-111 1.216644498076556 0.0011249134906767106 0.0 0.00118811087779338 2.5278954846667656E-5 112-113 1.4121993329010212 0.0011375529681000446 0.0 0.0011944306165050467 2.5278954846667656E-5 114-115 1.6334755042819706 0.0011596720535908787 0.0 0.0011975904858608803 2.5278954846667656E-5 116-117 1.8809248735372885 0.0011881108777933797 0.0 0.0012102299632842138 2.5278954846667656E-5 118-119 2.1502879367753116 0.0012070700939283805 0.0 0.0012133898326400475 2.5278954846667656E-5 120-121 2.4344455083373466 0.0012197095713517144 0.0 0.001216549701995881 2.5278954846667656E-5 122-123 2.7392243873155513 0.0012355089181308816 0.0 0.0012323490487750482 2.5278954846667656E-5 124-125 3.0764898831410794 0.0012765872197567165 0.0 0.0012671076116892162 2.5278954846667656E-5 126-127 3.4411008883719907 0.0013176655213825517 0.0 0.001349264214940886 2.5278954846667656E-5 128-129 3.8311362024399376 0.001355583953652553 0.0 0.0013587438230083866 2.5278954846667656E-5 130-131 4.237498561469476 0.0013650635617200534 0.0 0.0013650635617200534 2.843882420250111E-5 132-133 4.67091572192366 0.0013682234310758868 0.0 0.0013777030391433872 3.159869355833457E-5 134-135 5.133893460201667 0.0013777030391433872 0.0 0.0014156214714133887 3.159869355833457E-5 136-137 5.631177900075957 0.001390342516566721 0.0 0.001431420818192556 3.159869355833457E-5 138-139 6.156721051599465 0.0014187813407692221 0.0 0.001437740556904223 3.159869355833457E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCGGG 2215 0.0 12.780007 1 GTCGGCT 2560 0.0 12.758887 1 GTCGCGT 1145 1.8189894E-12 12.678409 1 GTCGTAT 2325 0.0 11.550984 1 GTCCCGT 2830 0.0 10.772167 1 GTCGTCT 4885 0.0 10.698137 1 GCCCGTT 4370 0.0 10.630134 1 GTCGGTT 4040 0.0 10.600122 1 GCCGTAT 2885 0.0 10.566806 1 CGTATGC 10620 0.0 10.444445 145 GTCCGTT 3020 0.0 10.334791 1 GCCCGCT 2130 0.0 10.223083 1 GTCCGGT 3820 0.0 10.070539 1 GCCCCGT 1960 0.0 9.998801 1 TATGCCG 8160 0.0 9.950532 145 GTCCGAT 3650 0.0 9.942999 1 ATGCCGT 9000 0.0 9.827334 145 GCCTTAT 11170 0.0 9.682183 1 TCGTATG 12105 0.0 9.642275 145 GTCACGT 33375 0.0 9.514178 145 >>END_MODULE SRR7171423 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7171423_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15823439 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0 30.0 30.0 30.0 30.0 30.0 2 30.0 30.0 30.0 30.0 30.0 30.0 3 30.0 30.0 30.0 30.0 30.0 30.0 4 30.0 30.0 30.0 30.0 30.0 30.0 5 30.0 30.0 30.0 30.0 30.0 30.0 6 30.0 30.0 30.0 30.0 30.0 30.0 7 30.0 30.0 30.0 30.0 30.0 30.0 8 30.0 30.0 30.0 30.0 30.0 30.0 9 30.0 30.0 30.0 30.0 30.0 30.0 10-14 30.0 30.0 30.0 30.0 30.0 30.0 15-19 30.0 30.0 30.0 30.0 30.0 30.0 20-24 30.0 30.0 30.0 30.0 30.0 30.0 25-29 30.0 30.0 30.0 30.0 30.0 30.0 30-34 30.0 30.0 30.0 30.0 30.0 30.0 35-39 30.0 30.0 30.0 30.0 30.0 30.0 40-44 30.0 30.0 30.0 30.0 30.0 30.0 45-49 30.0 30.0 30.0 30.0 30.0 30.0 50-54 30.0 30.0 30.0 30.0 30.0 30.0 55-59 30.0 30.0 30.0 30.0 30.0 30.0 60-64 30.0 30.0 30.0 30.0 30.0 30.0 65-69 30.0 30.0 30.0 30.0 30.0 30.0 70-74 30.0 30.0 30.0 30.0 30.0 30.0 75-79 30.0 30.0 30.0 30.0 30.0 30.0 80-84 30.0 30.0 30.0 30.0 30.0 30.0 85-89 30.0 30.0 30.0 30.0 30.0 30.0 90-94 30.0 30.0 30.0 30.0 30.0 30.0 95-99 30.0 30.0 30.0 30.0 30.0 30.0 100-104 30.0 30.0 30.0 30.0 30.0 30.0 105-109 30.0 30.0 30.0 30.0 30.0 30.0 110-114 30.0 30.0 30.0 30.0 30.0 30.0 115-119 30.0 30.0 30.0 30.0 30.0 30.0 120-124 30.0 30.0 30.0 30.0 30.0 30.0 125-129 30.0 30.0 30.0 30.0 30.0 30.0 130-134 30.0 30.0 30.0 30.0 30.0 30.0 135-139 30.0 30.0 30.0 30.0 30.0 30.0 140-144 30.0 30.0 30.0 30.0 30.0 30.0 145-149 30.0 30.0 30.0 30.0 30.0 30.0 150-151 30.0 30.0 30.0 30.0 30.0 30.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 1.5823439E7 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.74276066336109 19.463513149258773 15.462553771451306 28.33117241592883 2 25.991189706077588 25.948267144756798 30.544018627493042 17.516524521672572 3 20.62856322582589 28.567441125679988 30.423521170298873 20.380474478195254 4 23.629106256809333 33.71983869198551 23.416594030631256 19.234461020573907 5 24.396241428994234 35.33767794259911 22.58414288929639 17.681937739110268 6 20.76862078835183 37.364474878141216 23.525429151679862 18.341475181827093 7 20.200119026092388 21.279075426884113 38.49021294252741 20.030592604496093 8 22.19570475199043 25.21279030644753 27.570437737799008 25.02106720376303 9 21.958241103004056 25.261284276609334 29.736035056914318 23.044439563472295 10-14 23.579272923109144 28.689858048412276 26.214626859963918 21.51624216851466 15-19 23.270596944353624 27.955898594905126 27.621280320238345 21.152224140502902 20-24 23.2032600893793 28.186488575567193 27.49590308489654 21.114348250156976 25-29 23.380738017639416 28.13708587938909 27.45599151526959 21.026184587701902 30-34 23.164509778399907 28.106685750798 27.712994602817588 21.015809867984505 35-39 23.242589875525162 28.016681201163735 27.65649890046971 21.084230022841393 40-44 23.41575308456868 27.93326273112197 27.673627364264387 20.97735682004496 45-49 23.36597390880384 27.80767552220111 27.849564767012513 20.976785801982533 50-54 23.38528006969453 27.929767313468368 27.775064198994087 20.909888417843014 55-59 23.589549720948657 27.765819353201053 27.804231637675556 20.840399288174726 60-64 23.46931096899964 27.785247595340095 27.914543272419102 20.83089816324116 65-69 23.64358047192029 27.77904231561798 27.81762935104227 20.759747861419452 70-74 23.747123617219053 27.80477030712934 27.677169795771274 20.77093627988034 75-79 23.641356447365542 27.706377591552283 27.895102919305092 20.757163041777083 80-84 23.736827789238156 27.769891065108677 27.75307075027389 20.74021039537928 85-89 23.823754894984685 27.69418966988554 27.771766920868483 20.710288514261293 90-94 23.78045763965055 27.778180405987403 27.80549066057616 20.635871293785893 95-99 23.828196544927742 27.79041121465702 27.75674375803202 20.62464848238322 100-104 24.04444771955622 27.727680614251998 27.660868165143455 20.56700350104833 105-109 23.951021955884578 27.65246635465039 27.84379226170257 20.552719427762458 110-114 24.059020403088535 27.757206544710566 27.6355481850954 20.5482248671055 115-119 24.29431866863166 27.703938980546493 27.571538145755735 20.43020420506611 120-124 24.311314574432455 27.741646775966437 27.565914250743617 20.38112439885749 125-129 24.429020672569923 27.870270722932432 27.3919378615467 20.30877074295094 130-134 24.736269146764002 27.77681653732716 27.31319408452388 20.173720231384962 135-139 24.792975108798203 27.761274982901764 27.339899959877904 20.10584994842213 140-144 25.023828593486513 27.814208621846547 27.171552728214078 19.990410056452866 145-149 25.356511919279328 27.74758095853774 27.015003655350363 19.88090346683257 150-151 25.478979598209527 27.535199531840476 27.1113780967699 19.874442773180096 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 134.0 1 244.5 2 479.5 3 769.5 4 1006.0 5 1094.0 6 1086.0 7 1061.5 8 1042.0 9 969.0 10 910.0 11 953.0 12 990.5 13 1018.0 14 1059.0 15 1093.0 16 1162.0 17 1228.5 18 1341.5 19 1540.5 20 1795.0 21 2112.5 22 2640.5 23 3409.0 24 4364.5 25 5791.5 26 8056.0 27 11305.0 28 15996.0 29 23434.0 30 34379.0 31 50201.0 32 74452.0 33 109843.0 34 157123.0 35 218437.5 36 299033.5 37 399728.5 38 516979.5 39 646129.0 40 781185.5 41 909877.5 42 1019893.5 43 1097057.5 44 1135303.5 45 1138259.0 46 1102077.0 47 1033597.5 48 942894.5 49 825200.5 50 695507.0 51 578276.0 52 474482.0 53 376299.5 54 282720.0 55 207380.0 56 155865.5 57 117724.5 58 85179.0 59 61628.0 60 46773.5 61 35321.0 62 28513.0 63 23543.5 64 15922.0 65 9676.5 66 7304.5 67 6353.0 68 5413.0 69 4640.5 70 3783.0 71 2743.0 72 2034.5 73 1803.5 74 1349.0 75 763.0 76 590.5 77 658.0 78 638.5 79 307.0 80 121.0 81 72.0 82 56.5 83 40.5 84 33.0 85 36.5 86 30.5 87 24.5 88 24.0 89 21.5 90 18.0 91 15.0 92 16.5 93 14.0 94 10.5 95 10.5 96 6.0 97 4.0 98 7.5 99 6.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004322701278780169 2 0.1299401476505834 3 0.15073208801196755 4 0.15387299815166602 5 0.153209425586941 6 0.13224685228034183 7 0.14877928875006247 8 0.12917545926647173 9 0.15880871408547786 10-14 0.1844162953451522 15-19 0.18455532959680887 20-24 0.14621347483312572 25-29 0.10369174488554607 30-34 0.057594306774905256 35-39 0.04742079139686385 40-44 0.08919426428098216 45-49 0.14059522711845385 50-54 0.18200215515729545 55-59 0.19322348321373123 60-64 0.17802703950765697 65-69 0.12440658443464787 70-74 0.04947344253041327 75-79 0.012179400445124475 80-84 0.005720627481800891 85-89 0.04227146829459766 90-94 0.102315305794145 95-99 0.15672825610159713 100-104 0.2083529376894618 105-109 0.20194725053131624 110-114 0.21057242992499922 115-119 0.1754877684933092 120-124 0.1263315768462216 125-129 0.08258634548406323 130-134 0.07390302449423289 135-139 0.1212125884897714 140-144 0.1910343257240098 145-149 0.2078372470105898 150-151 0.2253492429806188 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 1.5823439E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.48456026966023 #Duplication Level Percentage of deduplicated Percentage of total 1 68.49300972571582 32.52360448371177 2 15.784705059428209 14.990595574664589 3 5.964036425094888 8.495989412336016 4 2.9229204492350456 5.551743689404955 5 1.7421566345041377 4.136277085515007 6 1.1014064053045494 3.137987930044422 7 0.7466809102935661 2.481907028092772 8 0.5739428419303129 2.180273877518401 9 0.4108113671246191 1.7556477409521438 >10 2.116936953917772 17.544606004335115 >50 0.10362374415017878 3.3206836285995656 >100 0.03796408324478546 3.043333075622482 >500 0.0013397365590527655 0.4194109520371284 >1k 4.656634970162485E-4 0.4179395171656275 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.84763419633368E-5 0.0 0.0 6.319738711666914E-6 0.0 2 8.84763419633368E-5 0.0 6.319738711666914E-6 4.42381709816684E-5 0.0 3 8.84763419633368E-5 0.0 6.319738711666914E-6 4.42381709816684E-5 0.0 4 9.479608067500371E-5 0.0 6.319738711666914E-6 5.687764840500223E-5 0.0 5 9.479608067500371E-5 0.0 6.319738711666914E-6 7.583686454000297E-5 0.0 6 9.479608067500371E-5 0.0 6.319738711666914E-6 1.0111581938667063E-4 0.0 7 1.0111581938667063E-4 0.0 6.319738711666914E-6 1.0111581938667063E-4 0.0 8 1.1375529681000446E-4 0.0 6.319738711666914E-6 1.0111581938667063E-4 0.0 9 1.1375529681000446E-4 0.0 6.319738711666914E-6 1.0111581938667063E-4 0.0 10-11 1.1375529681000446E-4 0.0 6.319738711666914E-6 1.1375529681000446E-4 0.0 12-13 1.1691516616583791E-4 0.0 1.2639477423333828E-5 1.1375529681000446E-4 0.0 14-15 1.2639477423333827E-4 0.0 1.2639477423333828E-5 1.1375529681000446E-4 0.0 16-17 1.3587438230083863E-4 0.0 1.2639477423333828E-5 1.2639477423333827E-4 0.0 18-19 1.4219412101250557E-4 0.0 1.2639477423333828E-5 1.2639477423333827E-4 0.0 20-21 1.5167372908000593E-4 0.0 1.2639477423333828E-5 1.2955464358917174E-4 0.0 22-23 1.5799346779167285E-4 0.0 1.2639477423333828E-5 1.4535399036833902E-4 0.0 24-25 1.611533371475063E-4 0.0 1.2639477423333828E-5 1.6747307585917323E-4 0.0 26-27 1.7063294521500668E-4 0.0 1.2639477423333828E-5 1.8011255328250706E-4 0.0 28-29 1.7063294521500668E-4 0.0 1.2639477423333828E-5 1.927520307058409E-4 0.0 30-31 1.769526839266736E-4 0.0 1.2639477423333828E-5 2.275105936200089E-4 0.0 32-33 1.8011255328250706E-4 0.0 1.2639477423333828E-5 2.7174876460167726E-4 0.0 34-35 2.0223163877334125E-4 0.0 1.2639477423333828E-5 3.001875888041784E-4 0.0 36-37 2.0223163877334125E-4 0.0 1.2639477423333828E-5 3.1282706622751225E-4 0.0 38-39 2.117112468408416E-4 6.319738711666914E-6 1.2639477423333828E-5 3.159869355833457E-4 0.0 40-41 2.4015007104334271E-4 1.5799346779167284E-5 1.2639477423333828E-5 3.223066742950126E-4 0.0 42-43 2.717487646016773E-4 2.843882420250111E-5 1.2639477423333828E-5 3.444257597858468E-4 0.0 44-45 3.286264130066795E-4 3.159869355833457E-5 1.2639477423333828E-5 3.570652372091806E-4 0.0 46-47 3.886639307675152E-4 3.159869355833457E-5 1.2639477423333828E-5 3.6022510656501407E-4 0.0 48-49 4.550211872400178E-4 3.159869355833457E-5 1.2639477423333828E-5 3.6970471463251446E-4 0.0 50-51 5.529771372708549E-4 3.4758562914168025E-5 1.2639477423333828E-5 3.9182380012334867E-4 0.0 52-53 7.172903437741946E-4 3.791843227000148E-5 1.2639477423333828E-5 4.4554157917251744E-4 0.0 54-55 9.068825051242021E-4 4.42381709816684E-5 1.5799346779167284E-5 5.024192275775196E-4 0.0 56-57 0.0012513082649100488 4.42381709816684E-5 1.895921613500074E-5 5.276981824241874E-4 0.0 58-59 0.0017252886682850674 4.42381709816684E-5 1.895921613500074E-5 5.940554388966898E-4 0.0 60-61 0.0022593065894209218 5.055790969333531E-5 1.895921613500074E-5 6.98331127639194E-4 0.0 62-63 0.0028912804605876127 5.055790969333531E-5 1.895921613500074E-5 7.236100824858616E-4 0.0 64-65 0.0038708399608959852 5.055790969333531E-5 1.895921613500074E-5 7.425692986208624E-4 0.0 66-67 0.005314900256511875 5.055790969333531E-5 1.895921613500074E-5 7.552087760441962E-4 0.0 68-69 0.007223461347435282 5.055790969333531E-5 1.895921613500074E-5 7.646883841116966E-4 0.0 70-71 0.009609162711089542 5.055790969333531E-5 1.895921613500074E-5 8.405252486516996E-4 0.0 72-73 0.013154536128334681 5.055790969333531E-5 1.895921613500074E-5 8.816035502775345E-4 0.0 74-75 0.017944898071778202 5.055790969333531E-5 1.895921613500074E-5 9.037226357683688E-4 0.0 76-77 0.024381551949610954 5.055790969333531E-5 1.895921613500074E-5 9.195219825475359E-4 0.0 78-79 0.03259089253606628 5.055790969333531E-5 1.895921613500074E-5 9.290015906150363E-4 0.0 80-81 0.04302794101838418 5.055790969333531E-5 1.895921613500074E-5 9.448009373942037E-4 0.0 82-83 0.05768341509073975 5.055790969333531E-5 1.895921613500074E-5 9.732397615967048E-4 0.0 84-85 0.076936499075833 5.055790969333531E-5 1.895921613500074E-5 9.858792390200386E-4 0.0 86-87 0.10184890907722398 5.055790969333531E-5 2.5278954846667653E-5 9.95358847087539E-4 0.0 88-89 0.13167807579629182 5.055790969333531E-5 3.159869355833457E-5 0.0010111581938667062 0.0 90-91 0.1674888752059524 5.055790969333531E-5 3.159869355833457E-5 0.0010143180632225398 0.0 92-93 0.21164804945372495 5.055790969333531E-5 3.159869355833457E-5 0.00102379767129004 0.0 94-95 0.2683677043909355 5.055790969333531E-5 3.159869355833457E-5 0.0010680358422717085 0.0 96-97 0.33750248602721566 5.055790969333531E-5 3.159869355833457E-5 0.0010680358422717085 0.0 98-99 0.41813287238001806 5.055790969333531E-5 3.159869355833457E-5 0.001099634535830043 0.0 100-101 0.5092919434264574 5.687764840500223E-5 3.159869355833457E-5 0.0011217536213208772 0.0 102-103 0.6155994281647624 5.687764840500223E-5 3.159869355833457E-5 0.0011754714003700461 0.0 104-105 0.7393715108327589 6.003751776083568E-5 3.159869355833457E-5 0.0011881108777933797 0.0 106-107 0.883872336475023 6.319738711666914E-5 3.159869355833457E-5 0.0012102299632842138 0.0 108-109 1.045638688277561 6.319738711666914E-5 3.159869355833457E-5 0.0012418286568425486 3.159869355833457E-6 110-111 1.2227051275010443 6.319738711666914E-5 3.159869355833457E-5 0.0012576280036217158 6.319738711666914E-6 112-113 1.4180134925157546 6.319738711666914E-5 3.159869355833457E-5 0.0012607878729775494 6.319738711666914E-6 114-115 1.638986316438544 6.951712582833605E-5 3.159869355833457E-5 0.0012702674810450496 6.319738711666914E-6 116-117 1.8868685877956115 7.583686454000297E-5 3.159869355833457E-5 0.0012765872197567165 6.319738711666914E-6 118-119 2.1571164144532675 7.583686454000297E-5 3.159869355833457E-5 0.0012987063052475507 6.319738711666914E-6 120-121 2.4431383089352448 7.583686454000297E-5 3.159869355833457E-5 0.0013176655213825517 6.319738711666914E-6 122-123 2.749746752270477 7.583686454000297E-5 3.159869355833457E-5 0.0013334648681617189 6.319738711666914E-6 124-125 3.0869616901863113 7.583686454000297E-5 3.159869355833457E-5 0.0013366247375175523 6.319738711666914E-6 126-127 3.451455780251057 7.583686454000297E-5 3.159869355833457E-5 0.0013429444762292192 6.319738711666914E-6 128-129 3.8416775266109977 7.583686454000297E-5 3.159869355833457E-5 0.0013524240842967194 6.319738711666914E-6 130-131 4.24765754144848 7.583686454000297E-5 3.159869355833457E-5 0.0013777030391433874 1.2639477423333828E-5 132-133 4.680547003720241 7.583686454000297E-5 3.159869355833457E-5 0.0013871826472108876 1.2639477423333828E-5 134-135 5.142978084599688 7.583686454000297E-5 3.159869355833457E-5 0.001402981993990055 1.2639477423333828E-5 136-137 5.6432454411458846 7.583686454000297E-5 3.159869355833457E-5 0.0014156214714133887 1.2639477423333828E-5 138-139 6.171619835612221 7.583686454000297E-5 3.4758562914168025E-5 0.0014251010794808891 1.2639477423333828E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTCGG 3320 0.0 21.198402 145 GCGGGAC 2185 0.0 13.276726 2 TACCCGC 2530 0.0 11.754484 9 CCGTGTA 16235 0.0 10.770459 145 GTTTAAT 15390 0.0 10.461503 1 GTTAGGC 7080 0.0 9.9361515 1 AGTAGTC 4905 0.0 9.020509 7 GTAGTCA 5415 0.0 8.97463 8 GTCTGAC 7200 0.0 8.662547 1 CGGGACT 3295 0.0 8.5845 3 TTAGGCT 8295 0.0 8.568251 2 GTGTAGA 19080 0.0 8.480002 145 ATCTCGG 11375 0.0 8.419611 145 GTAGCGC 1295 1.5633796E-6 8.401276 6 ATCCGTG 23535 0.0 8.354579 145 CTATAGC 9945 0.0 8.168709 9 AATCCGT 22395 0.0 7.969912 145 CGTGTAG 80600 0.0 7.9306793 145 CTCGGTG 10120 0.0 7.886452 145 CTACCCG 3990 0.0 7.816921 8 >>END_MODULE skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7171423 SRR7171423_1.fastq SRR7171423_2.fastq Input file: SRR7171423_1.fastq Paired file: SRR7171423_2.fastq trimmed: SRR7171423-trimmed-pair1.fastq, SRR7171423-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Apr 15 04:02:09 2025 >> started Tue Apr 15 04:02:29 2025 >> done (19.310s) 15823439 read pairs processed; of these: 13 ( 0.00%) short read pairs filtered out after trimming by size control 956 ( 0.01%) empty read pairs filtered out after trimming by size control 15822470 (99.99%) read pairs available; of these: 1637534 (10.35%) trimmed read pairs available after processing 14184936 (89.65%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 0 0.00% 20 1 0.00% 21 0 0.00% 22 0 0.00% 23 0 0.00% 24 1 0.00% 25 2 0.00% 26 0 0.00% 27 0 0.00% 28 0 0.00% 29 0 0.00% 30 0 0.00% 31 0 0.00% 32 1 0.00% 33 3 0.00% 34 0 0.00% 35 0 0.00% 36 0 0.00% 37 0 0.00% 38 3 0.00% 39 1 0.00% 40 5 0.00% 41 1 0.00% 42 4 0.00% 43 4 0.00% 44 6 0.00% 45 3 0.00% 46 7 0.00% 47 3 0.00% 48 8 0.00% 49 7 0.00% 50 12 0.00% 51 12 0.00% 52 18 0.00% 53 12 0.00% 54 20 0.00% 55 32 0.00% 56 31 0.00% 57 42 0.00% 58 37 0.00% 59 45 0.00% 60 44 0.00% 61 48 0.00% 62 63 0.00% 63 72 0.00% 64 113 0.00% 65 114 0.00% 66 126 0.00% 67 157 0.00% 68 168 0.00% 69 178 0.00% 70 242 0.00% 71 288 0.00% 72 323 0.00% 73 382 0.00% 74 458 0.00% 75 513 0.00% 76 586 0.00% 77 663 0.00% 78 749 0.00% 79 804 0.01% 80 1008 0.01% 81 1183 0.01% 82 1348 0.01% 83 1520 0.01% 84 1820 0.01% 85 2031 0.01% 86 2166 0.01% 87 2415 0.02% 88 2641 0.02% 89 2908 0.02% 90 3204 0.02% 91 3522 0.02% 92 4087 0.03% 93 4625 0.03% 94 5108 0.03% 95 5660 0.04% 96 6030 0.04% 97 6580 0.04% 98 6994 0.04% 99 7370 0.05% 100 7974 0.05% 101 8699 0.05% 102 9270 0.06% 103 10088 0.06% 104 10870 0.07% 105 11938 0.08% 106 12425 0.08% 107 13231 0.08% 108 13928 0.09% 109 14296 0.09% 110 15103 0.10% 111 15796 0.10% 112 16763 0.11% 113 18033 0.11% 114 19103 0.12% 115 20143 0.13% 116 21104 0.13% 117 21967 0.14% 118 22684 0.14% 119 23100 0.15% 120 24052 0.15% 121 24842 0.16% 122 26093 0.16% 123 27599 0.17% 124 28792 0.18% 125 29807 0.19% 126 30817 0.19% 127 32242 0.20% 128 32829 0.21% 129 33171 0.21% 130 34381 0.22% 131 35526 0.22% 132 36839 0.23% 133 37842 0.24% 134 39407 0.25% 135 40885 0.26% 136 42475 0.27% 137 43045 0.27% 138 43615 0.28% 139 44118 0.28% 140 44949 0.28% 141 45929 0.29% 142 47325 0.30% 143 48590 0.31% 144 50152 0.32% 145 52040 0.33% 146 52967 0.33% 147 54906 0.35% 148 55391 0.35% 149 55619 0.35% 150 56141 0.35% 151 14184936 89.65% 15822470 reads passed initial QC criterion=sequence-density sequence-density=0.16 sequence-density-rank=1 fanout-score=6.73 fanout-score-rank=13 prefix-density=0.30 prefix-fanout=3.7 sequence=TCCTTGTCCTGGATCTT criterion=fanout-score sequence-density=0.02 sequence-density-rank=30 fanout-score=90.35 fanout-score-rank=1 prefix-density=0.15 prefix-fanout=11.9 sequence=CTCCTTCCTCTAAATGATAAGGTTCAGTGGACTTCTCGCGACGTCGCCGGCGGCGAACCGCCCACGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGCTATAGACTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCTTGGCCTGGAAGGCCATAGTCCCTCTAAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCAT criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=2.68 fanout-score-rank=36 prefix-density=0.24 prefix-fanout=2.5 sequence=ATGTACCCTGACTT criterion=fanout-score sequence-density=0.03 sequence-density-rank=34 fanout-score=77.13 fanout-score-rank=1 prefix-density=0.23 prefix-fanout=10.3 sequence=TGGTGATGCAGTGCCTTGGTGCCATATGCGGTGCTGGTGTGGTGAAAGGATTTTACGGGAAAACAAACTACGAGTTGCATAATGGTGGTGCCAATATGGTCGCTCATGGTTACACCAAAGGTGATGGCCTTGGTGCTGAGATTGTTGG SRR7171423 testing PE reads STAR mapping to Ensembl genome Started job on | Apr 15 04:03:46 Started mapping on | Apr 15 04:03:52 Finished on | Apr 15 04:05:58 Mapping speed, Million of reads per hour | 452.07 Number of input reads | 15822470 Average input read length | 297 UNIQUE READS: Uniquely mapped reads number | 14694659 Uniquely mapped reads % | 92.87% Average mapped length | 296.44 Number of splices: Total | 14397333 Number of splices: Annotated (sjdb) | 14167815 Number of splices: GT/AG | 14180991 Number of splices: GC/AG | 171386 Number of splices: AT/AC | 10448 Number of splices: Non-canonical | 34508 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 2.36 Insertion rate per base | 0.02% Insertion average length | 2.59 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 381524 % of reads mapped to multiple loci | 2.41% Number of reads mapped to too many loci | 170171 % of reads mapped to too many loci | 1.08% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.38% % of reads unmapped: other | 0.27% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 746287 746287 746287 N_multimapping 381524 381524 381524 N_noFeature 313533 14573045 363814 N_ambiguous 136907 1142 64728 UnstrandedReadsAssigned:14244219 PositiveStrandReadsAssigned:120472 NegativeStrandReadsAssigned:14266117 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR7171423 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR7171423-trimmed-pair1.fastq SRR7171423-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,822,470 reads, 14,385,706 reads pseudoaligned [quant] estimated average fragment length: 231.876 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,147 rounds 52401 SRR7171423.ke.tsv 34699 SRR7171423.se.tsv 87100 total ==> SRR7171423.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1787.12 964 35.2402 Potri.005G024800.1.v4.1 1035 804.124 615 49.9653 Potri.004G059700.1.v4.1 961 730.124 12 1.07374 Potri.007G009000.2.v4.1 1416 1185.12 0 0 Potri.003G141000.2.v4.1 2943 2712.12 628.15 15.1311 Potri.016G087400.1.v4.1 270 80.4712 1145.76 930.184 Potri.015G069301.1.v4.1 564 335.964 0 0 Potri.010G195200.1.v4.1 1773 1542.12 145 6.14278 Potri.012G127500.1.v4.1 977 746.124 2210 193.507 ==> SRR7171423.se.tsv <== Potri.001G166300.v4.1 2 Potri.001G448400.v4.1 16 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 338 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 4 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 1 Potri.001G452600.v4.1 36 SRR7171423 completed mapping pipeline successfully