Starting /dee2/code/volunteer_pipeline.sh SRR7172127 current disk space = 3085229170688 free memory = 1489411512 SRR7172127 SRAfilesize f7f3cdc86bedb6c2b18456abe24d57f3 SRR7172127.sra SRR7172127.sra file validated SRR7172127 is paired end SRR7172127 is conventional basespace SRR7172127 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7172127_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.29825 32.0 18.0 33.0 18.0 33.0 2 27.3245 29.0 25.0 31.0 18.0 33.0 3 30.605 31.0 29.0 33.0 27.0 33.0 4 31.6235 33.0 31.0 33.0 29.0 33.0 5 32.383 33.0 33.0 33.0 32.0 33.0 6 36.95425 38.0 37.0 38.0 35.0 38.0 7 37.44 38.0 38.0 38.0 37.0 38.0 8 37.49775 38.0 38.0 38.0 38.0 38.0 9 37.63675 38.0 38.0 38.0 38.0 38.0 10-14 37.53975 38.0 38.0 38.0 38.0 38.0 15-19 37.560500000000005 38.0 38.0 38.0 38.0 38.0 20-24 37.5696 38.0 38.0 38.0 38.0 38.0 25-29 37.4272 38.0 38.0 38.0 38.0 38.0 30-34 37.31 38.0 38.0 38.0 37.2 38.0 35-39 37.3739 38.0 38.0 38.0 37.8 38.0 40-44 37.226749999999996 38.0 38.0 38.0 37.0 38.0 45-49 37.24405 38.0 38.0 38.0 37.0 38.0 50-54 37.0822 38.0 38.0 38.0 36.6 38.0 55-59 37.1694 38.0 38.0 38.0 37.0 38.0 60-64 37.201499999999996 38.0 38.0 38.0 37.0 38.0 65-69 37.148199999999996 38.0 38.0 38.0 37.0 38.0 70-74 37.0484 38.0 38.0 38.0 36.2 38.0 75-79 36.99035 38.0 38.0 38.0 36.0 38.0 80-84 36.88495 38.0 38.0 38.0 36.0 38.0 85-89 36.783699999999996 38.0 38.0 38.0 36.0 38.0 90-94 36.72195000000001 38.0 38.0 38.0 35.6 38.0 95-99 36.6263 38.0 38.0 38.0 35.0 38.0 100-104 36.432100000000005 38.0 38.0 38.0 34.0 38.0 105-109 36.3721 38.0 38.0 38.0 34.2 38.0 110-114 36.1195 38.0 38.0 38.0 34.0 38.0 115-119 35.73135 38.0 37.2 38.0 32.0 38.0 120-124 36.01135000000001 38.0 38.0 38.0 33.4 38.0 125-129 35.5815 38.0 36.8 38.0 31.4 38.0 130-134 35.557 38.0 36.4 38.0 31.2 38.0 135-139 35.3778 38.0 36.6 38.0 31.0 38.0 140-144 34.93769999999999 38.0 36.0 38.0 30.0 38.0 145-149 34.4486 38.0 36.0 38.0 28.2 38.0 150-151 30.050125 35.5 28.0 38.0 13.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 1.0 6 0.0 7 5.0 8 2.0 9 1.0 10 2.0 11 4.0 12 0.0 13 3.0 14 1.0 15 5.0 16 6.0 17 3.0 18 0.0 19 6.0 20 3.0 21 6.0 22 3.0 23 3.0 24 9.0 25 7.0 26 9.0 27 13.0 28 21.0 29 33.0 30 35.0 31 45.0 32 52.0 33 96.0 34 137.0 35 231.0 36 617.0 37 2640.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.449999999999996 14.274999999999999 18.675 31.6 2 23.474999999999998 18.675 40.775 17.075000000000003 3 20.7 28.325 29.675 21.3 4 22.7 34.849999999999994 24.275 18.175 5 22.95 32.7 28.849999999999998 15.5 6 16.25 36.25 29.049999999999997 18.45 7 13.725000000000001 21.65 45.675 18.95 8 18.525 24.05 30.25 27.175 9 19.75 20.8 35.125 24.325 10-14 20.83562672004003 30.297723292469353 26.36477358018514 22.50187640730548 15-19 20.935000000000002 28.68 28.425 21.959999999999997 20-24 20.5 28.87 28.895 21.735 25-29 21.12605630281514 28.381419070953545 28.61143057152858 21.881094054702736 30-34 20.95209520952095 29.38293829382938 27.642764276427645 22.022202220222024 35-39 22.484496899379877 29.010802160432085 27.430486097219443 21.074214842968594 40-44 21.988298244736708 29.23438515777367 26.829024353653047 21.948292243836576 45-49 22.188328249237387 29.324398659798973 27.604140621093165 20.883132469870482 50-54 20.84 29.494999999999997 27.295 22.37 55-59 20.169999999999998 30.930000000000003 26.765 22.134999999999998 60-64 20.715 28.685 28.360000000000003 22.24 65-69 21.325 29.48 27.134999999999998 22.06 70-74 22.23111155557778 28.781439071953596 27.301365068253414 21.68608430421521 75-79 20.945 30.220000000000002 26.72 22.115000000000002 80-84 22.664532906581318 28.030606121224245 27.565513102620525 21.739347869573912 85-89 21.473589435774308 28.061224489795915 29.056622649059623 21.408563425370147 90-94 20.8702175543886 28.637159289822456 29.08227056764191 21.410352588147035 95-99 21.30606530326516 28.496424821241064 28.71643582179109 21.481074053702685 100-104 21.27638291487446 27.998399519855955 27.598279483845158 23.126938081424427 105-109 20.95104755237762 28.356417820891046 27.51137556877844 23.181159057952897 110-114 20.984494957097695 29.038085202468768 27.226654623914897 22.750765216518644 115-119 21.326990485728594 28.793189784677015 26.73510265398097 23.144717075613418 120-124 22.09831474721208 29.189378406761012 27.124068610291545 21.58823823573536 125-129 22.261696272204155 29.15186389792344 26.755066299724795 21.83137353014761 130-134 22.051102555127756 29.42147107355368 26.88134406720336 21.646082304115204 135-139 21.44607230361518 29.736486824341217 26.756337816890845 22.06110305515276 140-144 22.275568892223056 29.342335583895974 25.291322830707674 23.090772693173292 145-149 22.03771319961987 29.475316360726257 25.288851097884258 23.19811934176962 150-151 23.0625 27.3375 25.687500000000004 23.9125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.0 2 1.5 3 1.5 4 1.5 5 0.5 6 0.5 7 1.5 8 1.5 9 1.0 10 0.5 11 1.0 12 1.5 13 0.5 14 0.5 15 1.0 16 0.5 17 0.0 18 0.5 19 2.5 20 3.0 21 2.0 22 3.0 23 2.5 24 2.5 25 4.0 26 5.5 27 9.5 28 10.5 29 20.0 30 37.0 31 49.5 32 59.5 33 101.0 34 148.5 35 182.0 36 215.0 37 221.0 38 236.0 39 217.5 40 164.0 41 132.5 42 114.5 43 115.5 44 127.5 45 136.0 46 143.0 47 140.5 48 146.0 49 151.5 50 138.5 51 125.0 52 135.5 53 142.5 54 108.5 55 82.0 56 66.0 57 59.0 58 57.5 59 48.0 60 36.0 61 25.0 62 19.0 63 13.0 64 9.0 65 6.0 66 3.0 67 1.5 68 1.0 69 1.0 70 0.5 71 0.0 72 0.0 73 1.0 74 1.0 75 0.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.075 15-19 0.0 20-24 0.0 25-29 0.005 30-34 0.01 35-39 0.02 40-44 0.015 45-49 0.015 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.005 75-79 0.0 80-84 0.02 85-89 0.04 90-94 0.025 95-99 0.005 100-104 0.03 105-109 0.005 110-114 0.35500000000000004 115-119 0.15 120-124 0.015 125-129 0.075 130-134 0.005 135-139 0.005 140-144 0.025 145-149 0.034999999999999996 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.175 #Duplication Level Percentage of deduplicated Percentage of total 1 85.88998975059788 62.849999999999994 2 7.174581482746841 10.5 3 2.801503245644004 6.15 4 1.7423983600956612 5.1 5 0.6149641270925863 2.25 6 0.47830543218312266 2.1 7 0.23915271609156133 1.225 8 0.17082336863682954 1.0 9 0.20498804236419543 1.35 >10 0.6832934745473181 7.475 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 27 0.675 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 22 0.5499999999999999 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 20 0.5 No Hit GCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAA 18 0.44999999999999996 No Hit GTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTA 18 0.44999999999999996 No Hit AAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAA 17 0.42500000000000004 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 16 0.4 No Hit GATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATG 15 0.375 No Hit TGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATA 14 0.35000000000000003 No Hit GTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATAC 14 0.35000000000000003 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 14 0.35000000000000003 No Hit CTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGA 13 0.325 No Hit TGGCACTCCAATGATTCCATATAACGGCCATAATGGAGCTATAGAATACA 13 0.325 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 12 0.3 No Hit ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC 12 0.3 No Hit CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC 12 0.3 No Hit GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT 11 0.27499999999999997 No Hit CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT 11 0.27499999999999997 No Hit TGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATA 10 0.25 No Hit TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG 10 0.25 No Hit TCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTC 9 0.22499999999999998 No Hit GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT 9 0.22499999999999998 No Hit CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT 9 0.22499999999999998 No Hit CTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTT 9 0.22499999999999998 No Hit ATTTTTTCCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATT 9 0.22499999999999998 No Hit TGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCA 9 0.22499999999999998 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 8 0.2 No Hit CGGCGCTCTTCCTAGTTGGTACAGAACTGAGTGTCATGTGTCCAAAGTTA 8 0.2 No Hit GCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG 8 0.2 No Hit GTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACC 8 0.2 No Hit CTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCAC 8 0.2 No Hit ATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGAC 7 0.17500000000000002 No Hit GCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGC 7 0.17500000000000002 No Hit TTTTTTTTTGGATTTTTTCCGCTTTGATATTCTCTGCATCCTATTTAGGG 7 0.17500000000000002 No Hit AGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATA 7 0.17500000000000002 No Hit CGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGAG 7 0.17500000000000002 No Hit GGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTA 7 0.17500000000000002 No Hit GGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAATATGAC 7 0.17500000000000002 No Hit CGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAAT 6 0.15 No Hit ATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGAT 6 0.15 No Hit GGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGG 6 0.15 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 6 0.15 No Hit CTGGAACCCAAAGGTTCGTTTTTTTCTTGGTACCTATTCCTCCAGGAATT 6 0.15 No Hit GGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAG 6 0.15 No Hit GTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTAGAAT 6 0.15 No Hit GGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTA 6 0.15 No Hit AAAATTCTTAACATTATTTTTAGAAATCCCATACTGCCACCGAATATTCA 6 0.15 No Hit CTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTT 6 0.15 No Hit CGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTT 6 0.15 No Hit CGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAAT 6 0.15 No Hit GTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGATACA 6 0.15 No Hit TCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCT 6 0.15 No Hit CTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTG 5 0.125 No Hit CGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCT 5 0.125 No Hit GGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGA 5 0.125 No Hit TTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAA 5 0.125 No Hit CACGAAACTAAGCGATTGAACTCTTGCCTGGTTACTGTATGCCCCTGTGT 5 0.125 No Hit CCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTA 5 0.125 No Hit AAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAAC 5 0.125 No Hit CAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAA 5 0.125 No Hit TTTTTTTTGGATTTTTTCCGCTTTGATATTCTCTGCATCCTATTTAGGGC 5 0.125 No Hit TTTTTTTGGATTTTTTCCGCTTTGATATTCTCTGCATCCTATTTAGGGCT 5 0.125 No Hit TTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAAC 5 0.125 No Hit GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT 5 0.125 No Hit TCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGT 5 0.125 No Hit CGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGT 5 0.125 No Hit CCGGGATCGGGCAAAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCA 5 0.125 No Hit GACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGC 5 0.125 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 5 0.125 No Hit TTTTTTTAAGGGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.037500000000000006 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.0625 0.0 0.0 0.0 0.0 86-87 0.1 0.0 0.0 0.0 0.0 88-89 0.175 0.0 0.0 0.0 0.0 90-91 0.375 0.0 0.0 0.0 0.0 92-93 0.4625 0.0 0.0 0.0 0.0 94-95 0.55 0.0 0.0 0.0 0.0 96-97 0.775 0.0 0.0 0.0 0.0 98-99 0.975 0.0 0.0 0.0 0.0 100-101 1.1625 0.0 0.0 0.0 0.0 102-103 1.3375 0.0 0.0 0.0 0.0 104-105 1.4625 0.0 0.0 0.0 0.0 106-107 1.75 0.0 0.0 0.0 0.0 108-109 2.0875 0.0 0.0 0.0 0.0 110-111 2.6 0.0 0.0 0.0 0.0 112-113 2.8875 0.0 0.0 0.0 0.0 114-115 3.3125 0.0 0.0 0.0 0.0 116-117 3.9125 0.0 0.0 0.0 0.0 118-119 4.35 0.0 0.0 0.0 0.0 120-121 4.825 0.0 0.0 0.0 0.0 122-123 5.4 0.0 0.0 0.0 0.0 124-125 5.85 0.0 0.0 0.0 0.0 126-127 6.5 0.0 0.0 0.0 0.0 128-129 7.137499999999999 0.0 0.0 0.0 0.0 130-131 7.7875 0.0 0.0 0.0 0.0 132-133 8.4625 0.0 0.0 0.0 0.0 134-135 9.462499999999999 0.0 0.0 0.0 0.0 136-137 10.4125 0.0 0.0 0.0 0.0 138-139 11.0375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGGAG 25 8.7132835E-4 87.0 145 CTACCAA 80 0.0018040554 36.25 8 >>END_MODULE SRR7172127 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7172127_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.09975 33.0 33.0 34.0 33.0 34.0 2 33.168 34.0 33.0 34.0 33.0 34.0 3 33.247 34.0 33.0 34.0 33.0 34.0 4 33.12575 34.0 33.0 34.0 33.0 34.0 5 33.09625 34.0 33.0 34.0 33.0 34.0 6 37.2745 38.0 38.0 38.0 37.0 38.0 7 37.2925 38.0 38.0 38.0 38.0 38.0 8 37.37825 38.0 38.0 38.0 38.0 38.0 9 37.3305 38.0 38.0 38.0 38.0 38.0 10-14 37.282650000000004 38.0 38.0 38.0 37.6 38.0 15-19 37.28304999999999 38.0 38.0 38.0 37.4 38.0 20-24 37.178250000000006 38.0 38.0 38.0 37.2 38.0 25-29 37.206900000000005 38.0 38.0 38.0 37.0 38.0 30-34 37.24055 38.0 38.0 38.0 37.4 38.0 35-39 37.19675 38.0 38.0 38.0 37.0 38.0 40-44 37.117650000000005 38.0 38.0 38.0 36.8 38.0 45-49 37.140100000000004 38.0 38.0 38.0 37.0 38.0 50-54 37.1719 38.0 38.0 38.0 37.0 38.0 55-59 37.148900000000005 38.0 38.0 38.0 37.0 38.0 60-64 37.10845 38.0 38.0 38.0 37.0 38.0 65-69 37.053399999999996 38.0 38.0 38.0 36.8 38.0 70-74 37.057900000000004 38.0 38.0 38.0 37.0 38.0 75-79 36.9893 38.0 38.0 38.0 36.4 38.0 80-84 36.86765 38.0 38.0 38.0 36.0 38.0 85-89 36.7529 38.0 38.0 38.0 35.8 38.0 90-94 36.5536 38.0 38.0 38.0 35.0 38.0 95-99 36.54105 38.0 38.0 38.0 35.0 38.0 100-104 36.29885 38.0 38.0 38.0 34.0 38.0 105-109 36.35955 38.0 38.0 38.0 34.0 38.0 110-114 36.1182 38.0 38.0 38.0 33.8 38.0 115-119 36.08155 38.0 38.0 38.0 33.6 38.0 120-124 35.8391 38.0 37.6 38.0 32.4 38.0 125-129 35.585 38.0 37.0 38.0 31.0 38.0 130-134 35.2684 38.0 36.2 38.0 30.0 38.0 135-139 34.87815 38.0 36.0 38.0 28.6 38.0 140-144 34.3942 38.0 35.8 38.0 26.8 38.0 145-149 33.15675 38.0 33.4 38.0 17.2 38.0 150-151 27.095375 33.0 17.5 37.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 1.0 4 2.0 5 3.0 6 0.0 7 1.0 8 1.0 9 2.0 10 1.0 11 3.0 12 4.0 13 2.0 14 1.0 15 0.0 16 2.0 17 3.0 18 7.0 19 3.0 20 3.0 21 5.0 22 7.0 23 12.0 24 4.0 25 13.0 26 20.0 27 20.0 28 26.0 29 31.0 30 38.0 31 53.0 32 63.0 33 83.0 34 131.0 35 206.0 36 554.0 37 2688.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.458364591147784 13.178294573643413 18.829707426856714 34.53363340835209 2 26.331582895723933 19.654913728432106 34.45861465366342 19.554888722180543 3 22.330582645661416 24.981245311327832 28.80720180045011 23.88097024256064 4 26.038019009504755 34.06703351675838 19.984992496248125 19.909954977488745 5 24.7935951963973 34.95121341005754 21.065799349512133 19.189392044033024 6 19.424280350438046 38.1476846057572 22.57822277847309 19.849812265331664 7 18.792282635930842 17.91530944625407 41.24279629165623 22.049611626158857 8 20.92092092092092 22.12212212212212 27.002002002002 29.954954954954953 9 21.952440550688358 22.57822277847309 28.88610763454318 26.583229036295368 10-14 23.217056203393224 28.10169661178119 25.213953255592813 23.46729392923277 15-19 24.21300235223462 27.22085981682599 27.82643511335769 20.739702717581704 20-24 24.514611689351483 27.321857485988794 26.095876701361085 22.067654123298638 25-29 25.431357839459867 27.001750437609402 26.676669167291823 20.89022255563891 30-34 23.25 26.66 28.000000000000004 22.09 35-39 23.36 26.729999999999997 28.605000000000004 21.305 40-44 24.67 27.265 26.99 21.075 45-49 22.444488897779554 25.365073014602917 30.61612322464493 21.574314862972592 50-54 21.16 26.275 29.53 23.035 55-59 22.82 26.69 29.439999999999998 21.05 60-64 21.795 27.63 29.515 21.060000000000002 65-69 20.955 29.01 28.02 22.015 70-74 21.305 27.950000000000003 28.53 22.215 75-79 21.349999999999998 28.24 28.935 21.475 80-84 22.33 27.93 28.89 20.849999999999998 85-89 21.634999999999998 29.555 28.675 20.135 90-94 21.75 28.9 28.725 20.625 95-99 21.86 29.89 27.560000000000002 20.69 100-104 21.029999999999998 29.2 27.139999999999997 22.63 105-109 21.64 29.675 27.644999999999996 21.04 110-114 21.21 30.055 26.845000000000002 21.89 115-119 22.66 30.91 27.215 19.215 120-124 22.46 29.035 26.915 21.59 125-129 22.62 29.415000000000003 28.595 19.37 130-134 23.400000000000002 27.889999999999997 27.63 21.08 135-139 23.035 28.95 27.925 20.09 140-144 24.905 28.01 28.345 18.740000000000002 145-149 24.474999999999998 27.57 27.66 20.294999999999998 150-151 27.425 26.0375 26.5875 19.950000000000003 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.0 22 1.5 23 2.5 24 1.5 25 0.5 26 1.5 27 7.0 28 9.5 29 9.5 30 23.0 31 45.5 32 55.5 33 59.5 34 78.0 35 99.5 36 135.5 37 195.5 38 281.5 39 276.0 40 202.0 41 151.0 42 120.5 43 135.5 44 134.5 45 127.0 46 132.5 47 147.5 48 166.0 49 158.5 50 127.5 51 124.5 52 150.0 53 164.5 54 144.0 55 112.0 56 96.5 57 75.0 58 66.0 59 60.5 60 38.5 61 22.5 62 18.5 63 15.0 64 9.0 65 4.5 66 2.0 67 1.5 68 1.0 69 2.0 70 1.0 71 0.0 72 0.0 73 0.5 74 1.5 75 1.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.025 3 0.025 4 0.05 5 0.075 6 0.125 7 0.22499999999999998 8 0.1 9 0.125 10-14 0.095 15-19 0.095 20-24 0.08 25-29 0.025 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.02 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.55 #Duplication Level Percentage of deduplicated Percentage of total 1 85.98881900768693 61.525 2 7.092941998602376 10.15 3 3.0747728860936405 6.6000000000000005 4 1.4675052410901468 4.2 5 0.7686932215234101 2.75 6 0.2445842068483578 1.05 7 0.3494060097833683 1.7500000000000002 8 0.1397624039133473 0.8 9 0.1397624039133473 0.8999999999999999 >10 0.6638714185883997 7.6 >50 0.06988120195667365 2.675 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCT 54 1.35 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 53 1.325 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 29 0.7250000000000001 No Hit TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC 27 0.675 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 22 0.5499999999999999 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 22 0.5499999999999999 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 20 0.5 No Hit CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA 16 0.4 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 15 0.375 No Hit TTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTACTATGTT 15 0.375 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 15 0.375 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 14 0.35000000000000003 No Hit TGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTT 14 0.35000000000000003 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 14 0.35000000000000003 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 13 0.325 No Hit GTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATAT 13 0.325 No Hit CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT 12 0.3 No Hit TTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGG 12 0.3 No Hit CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT 11 0.27499999999999997 No Hit CGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCA 10 0.25 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 10 0.25 No Hit GGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTA 9 0.22499999999999998 No Hit CTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTT 9 0.22499999999999998 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 9 0.22499999999999998 No Hit ATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTACTATGT 9 0.22499999999999998 No Hit GGCGTTTGTATCTGCCATTATAAAGAAGTTTCCTCCAGCAACTCCTTTCT 8 0.2 No Hit AAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGGA 8 0.2 No Hit CTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGC 8 0.2 No Hit GCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTTCTTAAA 8 0.2 No Hit CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA 7 0.17500000000000002 No Hit CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT 7 0.17500000000000002 No Hit GTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTGGAAAAGTC 7 0.17500000000000002 No Hit TATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCA 7 0.17500000000000002 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 7 0.17500000000000002 No Hit ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC 7 0.17500000000000002 No Hit CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC 7 0.17500000000000002 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 7 0.17500000000000002 No Hit TCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCG 7 0.17500000000000002 No Hit GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG 7 0.17500000000000002 No Hit CAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATA 6 0.15 No Hit TAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAA 6 0.15 No Hit GGAGAGGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATT 6 0.15 No Hit GTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAA 6 0.15 No Hit CCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGT 6 0.15 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 6 0.15 No Hit AGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT 6 0.15 No Hit ACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATC 5 0.125 No Hit CTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTA 5 0.125 No Hit TTTGGAGAGGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGC 5 0.125 No Hit CTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCGAAT 5 0.125 No Hit GTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCC 5 0.125 No Hit TTTTGCTGGTTTTTTGCGACGTTGGTGTTGTATTCTATAGCTCCATTATG 5 0.125 No Hit GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG 5 0.125 No Hit GTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGG 5 0.125 No Hit CTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCA 5 0.125 No Hit TAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTT 5 0.125 No Hit GTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTTCTAA 5 0.125 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 5 0.125 No Hit GTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCA 5 0.125 No Hit TTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTACTATGTTG 5 0.125 No Hit CTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTA 5 0.125 No Hit GGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCAAC 5 0.125 No Hit CACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTAC 5 0.125 No Hit TCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTA 5 0.125 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGAATCCGTGTAGATCT 5 0.125 Illumina Single End PCR Primer 1 (96% over 32bp) CGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAGACGAAT 5 0.125 No Hit CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG 5 0.125 No Hit CCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.037500000000000006 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.0625 0.0 0.0 0.0 0.0 86-87 0.1 0.0 0.0 0.0 0.0 88-89 0.175 0.0 0.0 0.0 0.0 90-91 0.375 0.0 0.0 0.0 0.0 92-93 0.4625 0.0 0.0 0.0 0.0 94-95 0.55 0.0 0.0 0.0 0.0 96-97 0.7625 0.0 0.0 0.0 0.0 98-99 0.95 0.0 0.0 0.0 0.0 100-101 1.15 0.0 0.0 0.0 0.0 102-103 1.3375 0.0 0.0 0.0 0.0 104-105 1.475 0.0 0.0 0.0 0.0 106-107 1.775 0.0 0.0 0.0 0.0 108-109 2.125 0.0 0.0 0.0 0.0 110-111 2.65 0.0 0.0 0.0 0.0 112-113 2.95 0.0 0.0 0.0 0.0 114-115 3.35 0.0 0.0 0.0 0.0 116-117 3.9375 0.0 0.0 0.0 0.0 118-119 4.3625 0.0 0.0 0.0 0.0 120-121 4.85 0.0 0.0 0.0 0.0 122-123 5.475 0.0 0.0 0.0 0.0 124-125 5.925 0.0 0.0 0.0 0.0 126-127 6.625 0.0 0.0 0.0 0.0 128-129 7.262499999999999 0.0 0.0 0.0 0.0 130-131 7.925 0.0 0.0 0.0 0.0 132-133 8.6125 0.0 0.0 0.0 0.0 134-135 9.575 0.0 0.0 0.0 0.0 136-137 10.5 0.0 0.0 0.0 0.0 138-139 11.1625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCCGC 10 0.006830828 145.0 7 ACCGCCG 10 0.006830828 145.0 6 GCCCTAA 35 0.0033124194 62.14286 7 CCTAACT 35 0.0033124194 62.14286 9 CTGAAAG 35 0.0033124194 62.14286 1 AAAGCCC 35 0.0033124194 62.14286 4 AAGCCCT 35 0.0033124194 62.14286 5 GAAAGCC 35 0.0033124194 62.14286 3 CCCTAAC 35 0.0033124194 62.14286 8 AGCCCTA 35 0.0033124194 62.14286 6 TGAAAGC 45 0.008957279 48.333332 2 TAACTTA 40 9.990927E-6 25.375 10-14 AACTTAA 40 2.9585467E-4 21.75 10-14 CTAACTT 40 2.9585467E-4 21.75 10-14 GAGGTAT 50 5.60876E-5 20.3 25-29 ACTTAAT 45 6.5511256E-4 19.333332 10-14 GACGGGA 55 1.1668232E-4 18.454546 20-24 TAATGGA 55 1.1668232E-4 18.454546 15-19 CGGGAGG 50 0.0013298223 17.4 20-24 AATGGAC 50 0.0013298223 17.4 15-19 >>END_MODULE Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448944 spots for SRR7172127.sra Written 448944 spots for SRR7172127.sra Read 448945 spots for SRR7172127.sra Written 448945 spots for SRR7172127.sra SRR ids: ['SRR7172127.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_t2wye1rw SRR7172127.sra spots: 8978881 blocks: [[1, 448944], [448945, 897888], [897889, 1346832], [1346833, 1795776], [1795777, 2244720], [2244721, 2693664], [2693665, 3142608], [3142609, 3591552], [3591553, 4040496], [4040497, 4489440], [4489441, 4938384], [4938385, 5387328], [5387329, 5836272], [5836273, 6285216], [6285217, 6734160], [6734161, 7183104], [7183105, 7632048], [7632049, 8080992], [8080993, 8529936], [8529937, 8978881]] SRR7172127 file size 3022942 SRR7172127 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7172127 SRR7172127_1.fastq SRR7172127_2.fastq Input file: SRR7172127_1.fastq Paired file: SRR7172127_2.fastq trimmed: SRR7172127-trimmed-pair1.fastq, SRR7172127-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Feb 14 06:29:43 2025 >> started Fri Feb 14 06:29:52 2025 >> done (9.385s) 8978881 read pairs processed; of these: 10074 ( 0.11%) short read pairs filtered out after trimming by size control 14469 ( 0.16%) empty read pairs filtered out after trimming by size control 8954338 (99.73%) read pairs available; of these: 5127764 (57.27%) trimmed read pairs available after processing 3826574 (42.73%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 8 0.00% 20 5 0.00% 21 7 0.00% 22 11 0.00% 23 10 0.00% 24 11 0.00% 25 8 0.00% 26 10 0.00% 27 8 0.00% 28 12 0.00% 29 10 0.00% 30 5 0.00% 31 16 0.00% 32 8 0.00% 33 7 0.00% 34 8 0.00% 35 3 0.00% 36 6 0.00% 37 3 0.00% 38 7 0.00% 39 14 0.00% 40 6 0.00% 41 9 0.00% 42 5 0.00% 43 12 0.00% 44 15 0.00% 45 13 0.00% 46 19 0.00% 47 20 0.00% 48 21 0.00% 49 25 0.00% 50 40 0.00% 51 34 0.00% 52 53 0.00% 53 36 0.00% 54 69 0.00% 55 71 0.00% 56 79 0.00% 57 68 0.00% 58 61 0.00% 59 110 0.00% 60 112 0.00% 61 120 0.00% 62 126 0.00% 63 146 0.00% 64 167 0.00% 65 201 0.00% 66 179 0.00% 67 220 0.00% 68 279 0.00% 69 313 0.00% 70 325 0.00% 71 403 0.00% 72 519 0.01% 73 526 0.01% 74 595 0.01% 75 715 0.01% 76 998 0.01% 77 1133 0.01% 78 1052 0.01% 79 1224 0.01% 80 1335 0.01% 81 1550 0.02% 82 1867 0.02% 83 2171 0.02% 84 3218 0.04% 85 3928 0.04% 86 5175 0.06% 87 6234 0.07% 88 6544 0.07% 89 6120 0.07% 90 6051 0.07% 91 5979 0.07% 92 6922 0.08% 93 6823 0.08% 94 7212 0.08% 95 7866 0.09% 96 8731 0.10% 97 8375 0.09% 98 9265 0.10% 99 9777 0.11% 100 10775 0.12% 101 11281 0.13% 102 12053 0.13% 103 12769 0.14% 104 13377 0.15% 105 14654 0.16% 106 16320 0.18% 107 18050 0.20% 108 19042 0.21% 109 18343 0.20% 110 18502 0.21% 111 19845 0.22% 112 20912 0.23% 113 26542 0.30% 114 24209 0.27% 115 26447 0.30% 116 27044 0.30% 117 26181 0.29% 118 27017 0.30% 119 27559 0.31% 120 29041 0.32% 121 30440 0.34% 122 31870 0.36% 123 32406 0.36% 124 32440 0.36% 125 35867 0.40% 126 36593 0.41% 127 36861 0.41% 128 37290 0.42% 129 39334 0.44% 130 40897 0.46% 131 41898 0.47% 132 43937 0.49% 133 44852 0.50% 134 46781 0.52% 135 47525 0.53% 136 53229 0.59% 137 51798 0.58% 138 55653 0.62% 139 60340 0.67% 140 65678 0.73% 141 67086 0.75% 142 71829 0.80% 143 74799 0.84% 144 83543 0.93% 145 94859 1.06% 146 119714 1.34% 147 150779 1.68% 148 225748 2.52% 149 441246 4.93% 150 2387070 26.66% 151 3826574 42.73% 8954338 reads passed initial QC criterion=sequence-density sequence-density=0.54 sequence-density-rank=1 fanout-score=22.43 fanout-score-rank=5 prefix-density=6.32 prefix-fanout=1.9 sequence=TTCGTTTTTTTTCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=143.65 fanout-score-rank=1 prefix-density=0.76 prefix-fanout=1.0 sequence=TTAATAGCCCCTAAGATAAACTATAATTGTTAAAATCTTAATGGAGGGAAACTATGGAAATAAATGGAGTATATATTGAAGATACATTTGCAGAAGCATTCCCAATATGGGTTTCAAGAGTTTTAATAACAGCAGCTACAAAGAAGTGGGCTAAGATTGCAGCTACAGAGGCAACAGGTTTTGGTTGTTCAGTTATAATGTGTCCAGCAGAAGCAGGAATTGAGAAATATGTCCCTCCATCAAAAACACCAGATGGAAGACCAGGATTTATAATACAGATATGCCACCCTAAAAAGTCAGAGTTAGAGCATCAAATGTTAGAGAGATTGGGGCAGTGTGTCTTAACATGTCCAACAACTGCTATTTTTGATGCTGTGGGAGACATGGCTGATGAGCAGTTAAAGGTTGGATTTAAGTTGAAGTTTTTCGGAGACGGTTATGAGAAGAAAGATGAATTATATGGAAGAAAAGTTTATAAAATCCCAATCATGGGAGGGGAATTTATAACTGA criterion=sequence-density sequence-density=1.13 sequence-density-rank=1 fanout-score=4.72 fanout-score-rank=8 prefix-density=5.25 prefix-fanout=1.0 sequence=CAAGAAAAAAAACGAA criterion=fanout-score sequence-density=0.10 sequence-density-rank=20 fanout-score=97.55 fanout-score-rank=1 prefix-density=10.16 prefix-fanout=1.0 sequence=GGTTTTCAAAAA SRR7172127 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 14 06:31:27 Started mapping on | Feb 14 06:31:27 Finished on | Feb 14 06:43:21 Mapping speed, Million of reads per hour | 45.15 Number of input reads | 8954338 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 2922849 Uniquely mapped reads % | 32.64% Average mapped length | 287.77 Number of splices: Total | 1632386 Number of splices: Annotated (sjdb) | 1578461 Number of splices: GT/AG | 1598456 Number of splices: GC/AG | 20360 Number of splices: AT/AC | 1380 Number of splices: Non-canonical | 12190 Mismatch rate per base, % | 0.44% Deletion rate per base | 0.07% Deletion average length | 2.50 Insertion rate per base | 0.04% Insertion average length | 2.25 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 82269 % of reads mapped to multiple loci | 0.92% Number of reads mapped to too many loci | 66286 % of reads mapped to too many loci | 0.74% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 65.45% % of reads unmapped: other | 0.25% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 5953236 5953236 5953236 N_multimapping 82269 82269 82269 N_noFeature 110310 2866079 128884 N_ambiguous 54515 339 16187 UnstrandedReadsAssigned:2758024 PositiveStrandReadsAssigned:56431 NegativeStrandReadsAssigned:2777778 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=148 echo kmer=143 SRR7172127 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR7172127-trimmed-pair1.fastq SRR7172127-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 8,954,338 reads, 2,832,434 reads pseudoaligned [quant] estimated average fragment length: 196.646 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,057 rounds 52401 SRR7172127.ke.tsv 34699 SRR7172127.se.tsv 87100 total ==> SRR7172127.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1822.35 189 33.2521 Potri.005G024800.1.v4.1 1035 839.354 14 5.34777 Potri.004G059700.1.v4.1 961 765.354 20 8.37833 Potri.007G009000.2.v4.1 1416 1220.35 0 0 Potri.003G141000.2.v4.1 2943 2747.35 105 12.2536 Potri.016G087400.1.v4.1 270 95.7533 176 589.316 Potri.015G069301.1.v4.1 564 368.956 0 0 Potri.010G195200.1.v4.1 1773 1577.35 119 24.1884 Potri.012G127500.1.v4.1 977 781.354 2199 902.333 ==> SRR7172127.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 46 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 598 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 152 SRR7172127 completed mapping pipeline successfully