Starting /dee2/code/volunteer_pipeline.sh SRR9668903 current disk space = 3051612835840 free memory = 1413633620 SRR9668903 SRAfilesize 1a1ee59efa04c8fb340d012a10a88f83 SRR9668903.sra SRR9668903.sra file validated SRR9668903 is paired end SRR9668903 is conventional basespace SRR9668903 read1 length is 35-76 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR9668903_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 35-76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.66775 32.0 32.0 32.0 32.0 32.0 2 31.6665 32.0 32.0 32.0 32.0 32.0 3 31.68275 32.0 32.0 32.0 32.0 32.0 4 31.691 32.0 32.0 32.0 32.0 32.0 5 31.70975 32.0 32.0 32.0 32.0 32.0 6 35.32625 36.0 36.0 36.0 36.0 36.0 7 35.37925 36.0 36.0 36.0 36.0 36.0 8 35.37 36.0 36.0 36.0 36.0 36.0 9 35.3945 36.0 36.0 36.0 36.0 36.0 10-11 35.2325 36.0 36.0 36.0 36.0 36.0 12-13 35.3405 36.0 36.0 36.0 36.0 36.0 14-15 35.34675 36.0 36.0 36.0 36.0 36.0 16-17 35.314 36.0 36.0 36.0 36.0 36.0 18-19 35.363625 36.0 36.0 36.0 36.0 36.0 20-21 35.260625000000005 36.0 36.0 36.0 36.0 36.0 22-23 35.262874999999994 36.0 36.0 36.0 36.0 36.0 24-25 35.16725 36.0 36.0 36.0 36.0 36.0 26-27 35.0965 36.0 36.0 36.0 36.0 36.0 28-29 35.129000000000005 36.0 36.0 36.0 36.0 36.0 30-31 35.127375 36.0 36.0 36.0 36.0 36.0 32-33 35.077875 36.0 36.0 36.0 36.0 36.0 34-35 35.111999999999995 36.0 36.0 36.0 36.0 36.0 36-37 34.9873655241431 36.0 36.0 36.0 36.0 36.0 38-39 35.044533400050035 36.0 36.0 36.0 36.0 36.0 40-41 35.11083312484363 36.0 36.0 36.0 36.0 36.0 42-43 35.1285964473355 36.0 36.0 36.0 36.0 36.0 44-45 34.89204403302477 36.0 36.0 36.0 36.0 36.0 46-47 34.89204403302477 36.0 36.0 36.0 36.0 36.0 48-49 34.98223802361281 36.0 36.0 36.0 36.0 36.0 50-51 34.91003503503504 36.0 36.0 36.0 36.0 36.0 52-53 34.77687687687688 36.0 36.0 36.0 32.0 36.0 54-55 34.667959949937426 36.0 36.0 36.0 32.0 36.0 56-57 34.74014642113357 36.0 36.0 36.0 32.0 36.0 58-59 34.61549574549653 36.0 36.0 36.0 32.0 36.0 60-61 34.6620741482966 36.0 36.0 36.0 32.0 36.0 62-63 34.63555061506863 36.0 36.0 36.0 32.0 36.0 64-65 34.64052769978694 36.0 36.0 36.0 32.0 36.0 66-67 34.63232893934584 36.0 36.0 36.0 32.0 36.0 68-69 34.430666870244764 36.0 36.0 36.0 32.0 36.0 70-71 34.336095463907405 36.0 36.0 36.0 32.0 36.0 72-73 34.29134142762352 36.0 36.0 36.0 32.0 36.0 74-75 34.209868503348666 36.0 36.0 36.0 32.0 36.0 76 33.52713178294574 36.0 32.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 0.0 20 3.0 21 2.0 22 3.0 23 4.0 24 2.0 25 13.0 26 12.0 27 31.0 28 41.0 29 52.0 30 69.0 31 81.0 32 126.0 33 191.0 34 513.0 35 2853.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.0868151113335 10.733049787340505 5.7042782086564925 34.475856892669505 2 20.915686765073804 13.660245183887914 36.82762071553665 28.596447335501622 3 19.73980485364023 18.413810357768327 26.594946209657245 35.2514385789342 4 25.74430823117338 26.319739804853644 21.51613710282712 26.419814861145856 5 23.54265699274456 31.22341756317238 22.692019014260694 22.54190642982237 6 21.215911933950462 30.09757317988491 24.668501376032022 24.0180135101326 7 16.412309231923945 18.739054290718038 42.90718038528897 21.94145609206905 8 19.189392044033024 18.21366024518389 31.423567675756818 31.17338003502627 9 19.239429572179134 19.089316987740805 32.12409306980235 29.54716037027771 10-11 24.20565424068051 26.444833625218916 22.329246935201404 27.020265198899175 12-13 23.455091318488865 21.816362271703778 27.09532149111834 27.633224918689013 14-15 23.68026019514636 23.35501626219665 26.45734300725544 26.507380535401552 16-17 23.14814814814815 23.636136136136134 26.25125125125125 26.964464464464466 18-19 24.34325744308231 23.067300475356518 25.093820365273956 27.495621716287218 20-21 22.34175631723793 24.380785589191895 25.31898924193145 27.958468851638727 22-23 24.7935951963973 23.317488116087066 25.106329747310486 26.782586940205157 24-25 22.95471603702777 23.755316487365523 24.96872654490868 28.32124093069802 26-27 23.129847385539154 22.829622216662496 25.719289467100324 28.32124093069802 28-29 23.405053790342755 23.229922441831373 24.36827620715537 28.996747560670507 30-31 23.14814814814815 22.57257257257257 26.95195195195195 27.32732732732733 32-33 22.029021766324743 21.816362271703778 26.507380535401552 29.647235426569928 34-35 23.29246935201401 23.892919689767325 26.494871153365025 26.319739804853644 36-37 22.27920940705529 24.530898173630224 25.106329747310486 28.083562672004003 38-39 23.53014761070803 22.466850137603203 25.156367275456592 28.84663497623217 40-41 23.667750813109834 23.855391543657746 24.305729296972732 28.171128346259692 42-43 23.642732049036777 23.204903677758317 26.54490868151113 26.607455591693768 44-45 21.79134350763072 23.792844633475106 26.53239929947461 27.88341255941956 46-47 23.01726294721041 22.86715036277208 26.8951713785339 27.220415311483613 48-49 21.643938446140375 23.508069560865756 24.659076692105593 30.188915300888276 50-51 20.995995995995994 24.71221221221221 26.063563563563562 28.22822822822823 52-53 22.575397321987236 24.32736828932549 26.304592666750093 26.792641721937176 54-55 23.27909887359199 25.444305381727162 24.893617021276597 26.382978723404253 56-57 20.766053323319564 23.48228814620103 27.024658905995746 28.72699962448366 58-59 21.885564041567548 22.899712032052086 26.60573431826718 28.608989608113184 60-61 23.997995991983966 24.06062124248497 24.66182364729459 27.279559118236474 62-63 21.280862263441534 22.922672014036845 27.321719513723526 28.474746208798095 64-65 20.707387432584976 22.91483757682177 27.568042142230027 28.809732848363222 66-67 22.449491780650018 24.79608482871126 25.109800476847788 27.64462291379094 68-69 21.584630838774483 24.74886991461577 25.42692114515319 28.23957810145655 70-71 21.953979630328178 23.902929712058345 26.078209480699105 28.064881176914376 72-73 23.048749684263704 24.324324324324326 25.448345541803484 27.178580449608486 74-75 22.348081023454156 22.294776119402986 24.933368869936036 30.423773987206825 76 26.245847176079735 0.0 37.098560354374314 36.65559246954596 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3.0 1 1.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.0 22 1.0 23 3.5 24 4.5 25 2.5 26 2.0 27 4.5 28 9.5 29 11.5 30 9.5 31 10.0 32 15.5 33 23.0 34 32.5 35 36.5 36 54.0 37 76.5 38 74.0 39 71.5 40 80.0 41 100.0 42 117.5 43 142.5 44 160.0 45 180.5 46 213.5 47 248.0 48 256.0 49 276.5 50 296.0 51 266.0 52 251.0 53 239.5 54 240.5 55 255.0 56 261.0 57 247.0 58 228.5 59 209.5 60 177.5 61 113.0 62 68.0 63 62.5 64 45.5 65 33.0 66 29.0 67 25.5 68 21.0 69 11.0 70 5.0 71 4.5 72 8.5 73 14.0 74 7.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 1.0 96 2.0 97 1.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.075 3 0.075 4 0.075 5 0.075 6 0.075 7 0.075 8 0.075 9 0.075 10-11 0.075 12-13 0.075 14-15 0.075 16-17 0.1 18-19 0.075 20-21 0.075 22-23 0.075 24-25 0.075 26-27 0.075 28-29 0.075 30-31 0.1 32-33 0.075 34-35 0.075 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 35 3.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 1.0 49 0.0 50 0.0 51 0.0 52 1.0 53 0.0 54 0.0 55 0.0 56 1.0 57 0.0 58 1.0 59 1.0 60 0.0 61 2.0 62 1.0 63 1.0 64 3.0 65 0.0 66 1.0 67 1.0 68 2.0 69 4.0 70 1.0 71 8.0 72 18.0 73 73.0 74 250.0 75 918.0 76 2709.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.3 #Duplication Level Percentage of deduplicated Percentage of total 1 85.1925630810093 64.14999999999999 2 8.233731739707835 12.4 3 3.0212483399734396 6.825 4 1.1952191235059761 3.5999999999999996 5 0.9960159362549801 3.75 6 0.49800796812749004 2.25 7 0.199203187250996 1.05 8 0.16600265604249667 1.0 9 0.06640106241699867 0.44999999999999996 >10 0.4316069057104913 4.5249999999999995 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGC 22 0.5499999999999999 No Hit GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTCCACC 19 0.475 No Hit GTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCAT 18 0.44999999999999996 No Hit GTCGAGTTATCATGAATCATCAGAGCAACGGGCAGAGCCCGCGTCGACCTTTTATCTAATAAATGCGTCCCTTCC 17 0.42500000000000004 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 14 0.35000000000000003 No Hit GTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCAT 13 0.325 No Hit CCAGAACCCAAAAACTTTGATTTCTCATAAGGTGCTGGCGGAGTCCTAAA 12 0.3 No Hit ATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGCTATAG 12 0.3 No Hit GTCGGCAATCGGACGGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCA 11 0.27499999999999997 No Hit GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG 11 0.27499999999999997 No Hit CGGCAATCGGACGGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGACT 11 0.27499999999999997 No Hit GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT 11 0.27499999999999997 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAACCCA 10 0.25 No Hit GTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCA 9 0.22499999999999998 No Hit CAGAAATTTGAATGATGCGTCGCCAGCACGAAGGCCGTGCGATCCGTCGA 9 0.22499999999999998 No Hit CTCTAAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAGCTAGTTAGCAGG 8 0.2 No Hit GTCAGTATCGCTGCGGGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGC 8 0.2 No Hit GTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCT 8 0.2 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 8 0.2 No Hit GCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACG 8 0.2 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTACAGCACTGC 7 0.17500000000000002 No Hit GCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGG 7 0.17500000000000002 No Hit GTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACTTC 7 0.17500000000000002 No Hit GTTTACGGCTAGGACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCT 7 0.17500000000000002 No Hit CTCAAACTTCCTTGGCCTGGAAGGCCATAGTCCCTCTAAGAAGCTGGCCG 7 0.17500000000000002 No Hit CCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCTCTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTG 7 0.17500000000000002 No Hit GCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTA 6 0.15 No Hit CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGT 6 0.15 No Hit GTCATATCTAGTATTCAGAGTTTGCCTCGATTTGGTACCGCTCTCGCGGC 6 0.15 No Hit CCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATCCA 6 0.15 No Hit GCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATC 6 0.15 No Hit GTTCCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGA 6 0.15 No Hit CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTT 6 0.15 No Hit CTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATT 6 0.15 No Hit GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGTC 6 0.15 No Hit GGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTC 6 0.15 No Hit GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGGACTTCTCGCG 6 0.15 No Hit GTTCAGTCATAATCCAACGCACGGTAGCTTCGCGCCACTGGCTTTTCAAC 6 0.15 No Hit GTCATTGCTTCTTCTCCGGGAAAAGAAGTTCACGACCCGTAGGCCTTCTACCTCCACGCGGCATTGCTCCGTCA 6 0.15 No Hit GGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTCTTTCGCC 6 0.15 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTGAAGACCAACA 6 0.15 No Hit CGATAGAACTCGCACCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATT 5 0.125 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 5 0.125 No Hit CCAGTATCCATCGTTTACGGCTAGGACTACTGGGGTATCTAATCCCATTC 5 0.125 No Hit CCGGAATCGAACCCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCT 5 0.125 No Hit GCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAG 5 0.125 No Hit CTTTTATCTAATAAATGCGTCCCTTCCAGAAGTCGGGGTTTGTTGCACGT 5 0.125 No Hit CGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGA 5 0.125 No Hit GTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAATCAAGAA 5 0.125 No Hit CTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATG 5 0.125 No Hit CACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGCTATAGAC 5 0.125 No Hit CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA 5 0.125 No Hit GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATC 5 0.125 No Hit GGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTTGGTAAGCTAT 5 0.125 No Hit GGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTCTTTCGC 5 0.125 No Hit GGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAA 5 0.125 No Hit GTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAG 5 0.125 No Hit CCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCC 5 0.125 No Hit CTCATCTTGGGGTGGGCTTACTACTTAGATGCTTTCAGCAGTTATCCGCT 5 0.125 No Hit GTTCACCATCTTTCGGGTCCCGACAGGCATGCTCTCACTCGAACCCTTCT 5 0.125 No Hit GATAGAACTCGCACCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGA 5 0.125 No Hit GCCTTTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAG 5 0.125 No Hit AAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAGCTAGTTAGCAGGCTGA 5 0.125 No Hit GCATCACAGACCTGTTATTGCCTCAAACTTCCTTGGCCTGGAAGGCCATAGTCCCTCTAAGAAGCTGGCCGCGG 5 0.125 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCATCCTCTCGGACCAGCT 5 0.125 No Hit GGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTA 5 0.125 No Hit GTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA 5 0.125 No Hit CGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTA 5 0.125 No Hit GTCCAACTACGAGCTTTTTAACTGCAACAACTTAAATATACGCTATTGGA 5 0.125 No Hit GCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGT 5 0.125 No Hit CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 41 0.0 0.0 0.0 0.0 0.0 42 0.0 0.0 0.0 0.0 0.0 43 0.0 0.0 0.0 0.0 0.0 44 0.0 0.0 0.0 0.0 0.0 45 0.0 0.0 0.0 0.0 0.0 46 0.0 0.0 0.0 0.0 0.0 47 0.0 0.0 0.0 0.0 0.0 48 0.0 0.0 0.0 0.0 0.0 49 0.0 0.0 0.0 0.0 0.0 50 0.0 0.0 0.0 0.0 0.0 51 0.0 0.0 0.0 0.0 0.0 52 0.0 0.0 0.0 0.0 0.0 53 0.0 0.0 0.0 0.0 0.0 54 0.0 0.0 0.0 0.0 0.0 55 0.0 0.0 0.0 0.0 0.0 56 0.0 0.0 0.0 0.0 0.0 57 0.0 0.0 0.0 0.0 0.0 58 0.0 0.0 0.0 0.0 0.0 59 0.0 0.0 0.0 0.0 0.0 60 0.0 0.0 0.0 0.0 0.0 61 0.0 0.0 0.0 0.0 0.0 62 0.0 0.0 0.0 0.0 0.0 63 0.0 0.0 0.0 0.0 0.0 64 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTCTTG 20 0.006555495 52.303123 54 >>END_MODULE SRR9668903 read2 length is 35-76 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR9668903_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 35-76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.2265 32.0 32.0 32.0 32.0 32.0 2 31.14025 32.0 32.0 32.0 32.0 32.0 3 31.17675 32.0 32.0 32.0 32.0 32.0 4 31.16475 32.0 32.0 32.0 32.0 32.0 5 31.169 32.0 32.0 32.0 32.0 32.0 6 34.71775 36.0 36.0 36.0 36.0 36.0 7 34.66275 36.0 36.0 36.0 32.0 36.0 8 34.55175 36.0 36.0 36.0 32.0 36.0 9 34.561 36.0 36.0 36.0 32.0 36.0 10-11 34.589375000000004 36.0 36.0 36.0 34.0 36.0 12-13 34.555125000000004 36.0 36.0 36.0 32.0 36.0 14-15 34.587375 36.0 36.0 36.0 36.0 36.0 16-17 34.474000000000004 36.0 36.0 36.0 32.0 36.0 18-19 34.471625 36.0 36.0 36.0 32.0 36.0 20-21 34.356375 36.0 36.0 36.0 32.0 36.0 22-23 34.279624999999996 36.0 36.0 36.0 32.0 36.0 24-25 34.28525 36.0 36.0 36.0 32.0 36.0 26-27 34.255250000000004 36.0 36.0 36.0 32.0 36.0 28-29 34.225875 36.0 36.0 36.0 32.0 36.0 30-31 34.1755 36.0 36.0 36.0 32.0 36.0 32-33 34.15025 36.0 36.0 36.0 32.0 36.0 34-35 34.078 36.0 36.0 36.0 32.0 36.0 36-37 34.10057543157368 36.0 36.0 36.0 32.0 36.0 38-39 34.079809857393045 36.0 36.0 36.0 32.0 36.0 40-41 33.99524643482612 36.0 36.0 36.0 32.0 36.0 42-43 34.022767075306476 36.0 36.0 36.0 32.0 36.0 44-45 33.91506129597198 36.0 36.0 36.0 29.5 36.0 46-47 33.96897673254941 36.0 36.0 36.0 32.0 36.0 48-49 33.97598026472808 36.0 36.0 36.0 32.0 36.0 50-51 33.88488488488488 36.0 36.0 36.0 32.0 36.0 52-53 33.896630078138216 36.0 36.0 36.0 29.5 36.0 54-55 33.83254067584481 36.0 36.0 36.0 32.0 36.0 56-57 33.947307600950865 36.0 36.0 36.0 32.0 36.0 58-59 33.830846100104324 36.0 36.0 36.0 32.0 36.0 60-61 33.7686623246493 36.0 36.0 36.0 27.0 36.0 62-63 33.71802804431213 36.0 36.0 36.0 27.0 36.0 64-65 33.76946721581984 36.0 36.0 36.0 27.0 36.0 66-67 33.64296463506396 36.0 36.0 36.0 27.0 36.0 68-69 33.6776420786004 36.0 36.0 36.0 27.0 36.0 70-71 33.645144713772325 36.0 36.0 36.0 27.0 36.0 72-73 33.60008681463175 36.0 36.0 36.0 27.0 36.0 74-75 33.56472652428241 36.0 36.0 36.0 27.0 36.0 76 32.948178745775444 36.0 32.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 3.0 15 25.0 16 30.0 17 27.0 18 20.0 19 24.0 20 15.0 21 18.0 22 20.0 23 19.0 24 18.0 25 17.0 26 21.0 27 30.0 28 44.0 29 56.0 30 65.0 31 88.0 32 115.0 33 203.0 34 470.0 35 2669.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.01203610832498 18.35506519558676 6.318956870611836 21.31394182547643 2 33.7171464330413 22.528160200250312 26.633291614518146 17.121401752190238 3 28.921691268451337 23.642732049036777 25.31898924193145 22.116587440580435 4 30.92319239429572 31.973980485364024 17.91343507630723 19.189392044033024 5 33.55016262196647 32.12409306980235 16.737553164873656 17.588191143357516 6 28.271203402551915 32.849637227920944 18.88916687515637 19.989992494370778 7 27.32049036777583 17.91343507630723 32.47435576682512 22.291718789091817 8 27.270452839629723 21.015761821366024 24.31823867900926 27.395546659994995 9 29.19689767325494 21.79134350763072 25.293970477858394 23.71778834125594 10-11 29.509632224168126 28.67150362772079 19.552164123092318 22.266700025018764 12-13 30.185138854140604 22.441831373530146 23.430072554415812 23.942957217913435 14-15 29.497122842131603 25.068801601200903 24.68101075806855 20.75306479859895 16-17 29.00925694270703 25.181386039529645 23.8804103077308 21.928946710032523 18-19 29.934951213410056 25.444083062296723 23.15486614961221 21.46609957468101 20-21 29.422066549912433 24.36827620715537 23.417563172379285 22.792094070552913 22-23 29.68476357267951 25.01876407305479 23.079809857393045 22.216662496872654 24-25 29.83487615711784 25.281461095821868 23.254941205904426 21.62872154115587 26-27 29.72229171878909 25.606705028771582 22.854640980735553 21.816362271703778 28-29 30.035026269702275 26.770077558168627 22.34175631723793 20.853139854891168 30-31 30.960720540405305 25.381536152114087 22.004003002251686 21.653740305228922 32-33 28.083562672004003 26.945208906680012 22.466850137603203 22.504378283712782 34-35 29.484613460095073 26.207155366524894 23.267450587940957 21.04078058543908 36-37 29.00925694270703 26.832624468351263 22.0540405303978 22.10407805854391 38-39 28.621466099574683 26.482361771328495 22.216662496872654 22.679509632224168 40-41 29.30948211158369 26.782586940205157 22.516887665749312 21.391043282461847 42-43 29.472104078058543 25.494120590442833 23.217413059794847 21.816362271703778 44-45 28.87165374030523 26.26970227670753 23.00475356517388 21.85389041781336 46-47 29.12184138103578 26.057042782086565 22.9672254190643 21.85389041781336 48-49 28.637557863130237 26.82347053671963 22.407106217940697 22.131865382209433 50-51 28.803803803803802 27.014514514514516 22.62262262262262 21.55905905905906 52-53 29.65836566136904 25.916656238268054 22.975847828807407 21.4491302715555 54-55 30.100125156445557 26.821026282853566 22.14017521902378 20.9386733416771 56-57 29.215170859932403 26.186005757917137 23.30704718988609 21.291776192264365 58-59 28.258419932390133 25.591586327782643 24.139226242644295 22.010767497182922 60-61 30.210420841683366 25.613727454909817 22.85821643286573 21.317635270541082 62-63 29.500187899285983 25.9050482274834 22.38506827007391 22.209695603156707 64-65 30.086498683715686 25.974677196941204 22.878275040742132 21.060549078600978 66-67 29.031853523952844 26.950087785302234 21.8209179834462 22.197140707298722 68-69 28.180677540777914 26.135508155583437 23.588456712672524 22.09535759096612 70-71 29.292802411757318 26.303228237658587 21.76862203240799 22.63534731817611 72-73 28.382630585273755 26.356198867212083 22.177470106985524 23.083700440528634 74-75 30.02810116419109 22.521075873143314 24.702261474642047 22.74856148802355 76 31.543372136687946 0.0 35.260983852797594 33.19564401051446 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 1.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 0.0 23 0.5 24 1.5 25 2.0 26 3.0 27 4.5 28 4.5 29 5.0 30 6.0 31 6.5 32 12.5 33 22.5 34 34.0 35 39.5 36 49.5 37 64.0 38 74.0 39 97.0 40 107.0 41 125.5 42 153.5 43 167.0 44 189.5 45 205.5 46 215.5 47 245.5 48 274.5 49 257.0 50 234.0 51 214.5 52 200.0 53 193.5 54 192.5 55 217.0 56 234.0 57 227.5 58 209.0 59 171.0 60 137.0 61 117.0 62 101.5 63 73.5 64 42.0 65 39.0 66 31.5 67 23.0 68 26.5 69 28.0 70 20.0 71 14.5 72 14.0 73 15.5 74 11.5 75 4.5 76 6.0 77 7.0 78 6.0 79 7.0 80 6.0 81 3.5 82 2.5 83 1.5 84 1.5 85 3.5 86 4.5 87 4.0 88 3.5 89 3.0 90 3.5 91 4.0 92 4.0 93 3.5 94 3.0 95 1.5 96 0.0 97 1.0 98 4.0 99 21.0 100 36.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3 2 0.125 3 0.075 4 0.075 5 0.075 6 0.075 7 0.075 8 0.075 9 0.075 10-11 0.075 12-13 0.075 14-15 0.075 16-17 0.075 18-19 0.075 20-21 0.075 22-23 0.075 24-25 0.075 26-27 0.075 28-29 0.075 30-31 0.075 32-33 0.075 34-35 0.075 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 35 3.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 1.0 49 0.0 50 0.0 51 0.0 52 1.0 53 0.0 54 0.0 55 0.0 56 1.0 57 0.0 58 1.0 59 1.0 60 0.0 61 0.0 62 1.0 63 1.0 64 3.0 65 0.0 66 0.0 67 1.0 68 2.0 69 3.0 70 1.0 71 5.0 72 5.0 73 66.0 74 335.0 75 906.0 76 2663.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.60000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 85.97328244274809 67.575 2 9.001272264631044 14.149999999999999 3 2.6399491094147582 6.225 4 1.049618320610687 3.3000000000000003 5 0.5725190839694656 2.25 6 0.34987277353689566 1.6500000000000001 7 0.09541984732824427 0.525 8 0.09541984732824427 0.6 9 0.031806615776081425 0.22499999999999998 >10 0.19083969465648853 3.5000000000000004 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 45 1.125 No Hit GTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCAAGGAAGCTGACTGGCGGGATCCCCTCG 26 0.65 No Hit ACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 23 0.575 No Hit GTTAGTTTTACCCTACTGATGACAGTGTCGCAATAGTAATCCAACCTAGTACGAGAGGAACCGTTGATTCGCACA 19 0.475 No Hit GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTA 17 0.42500000000000004 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 10 0.25 No Hit CAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTC 9 0.22499999999999998 No Hit GTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTA 8 0.2 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAA 8 0.2 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAA 8 0.2 No Hit GTTCGAGTGAGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTATGCC 7 0.17500000000000002 No Hit CTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCAAGGAA 7 0.17500000000000002 No Hit CAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACTAATTCAGACTGTGAAACTGCG 7 0.17500000000000002 No Hit GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC 6 0.15 No Hit GTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAA 6 0.15 No Hit GGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGG 6 0.15 No Hit GGAGTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCA 6 0.15 No Hit GTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATAC 6 0.15 No Hit GGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTC 6 0.15 No Hit GTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCAAGGAAGCTGACTGG 6 0.15 No Hit GTTCTATCGGGTAAAGCCAATGATTAGAGGCATCGGGGGCGCAACGCCCT 6 0.15 No Hit ATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAACTG 6 0.15 No Hit GGCTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCGACGAAATGCTTCGGGGAGTTGAA 6 0.15 No Hit GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTA 6 0.15 No Hit GTGCAACAAACCCCGACTTCTGGAAGGGACGCATTTATTAGATAAAAGGT 5 0.125 No Hit GTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGGGGGCATT 5 0.125 No Hit AGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTCTGGAAGGGAC 5 0.125 No Hit GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC 5 0.125 No Hit GGGAAACAGCCCGGATCACCAGCTAAGGCCCCTAAATGACCGCTCAGTGATAAAGGAGGTAGGGGTGCAGAGAC 5 0.125 No Hit GTCGCCTCGTCCCTTCTACCGGCGATGCGCTCCTGGCCTTAACTGGCCGG 5 0.125 No Hit AAACCCGTAAGGCGCAAGGAAGCTGACTGGCGGGATCCCCTCGAGGGTTG 5 0.125 No Hit GGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCC 5 0.125 No Hit GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACTA 5 0.125 No Hit GCTTCTTAGAGGGACTATGGCCTTCCAGGCCAAGGAAGTTTGAGGCAATA 5 0.125 No Hit GGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCA 5 0.125 No Hit GTTTGTTTGATGGTATTTGCTACTCGGATAACCGTAGTAATTCTAGAGCT 5 0.125 No Hit GCAGAATTCACCAAGTGTTGGATTGTTCACCCACCAATAGGGAACGTGAG 5 0.125 No Hit GGTAAAGCCAATGATTAGAGGCATCGGGGGCGCAACGCCCTCGACCTATT 5 0.125 No Hit GTATGAACTAATTCAGACTGTGAAACTGCGAATGGCTCATTAAATCAGTT 5 0.125 No Hit GTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTCTGGAAGGGACG 5 0.125 No Hit GTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGA 5 0.125 No Hit GGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAGGCGATGATCAGTAGCTGGTCCGAGAGGATG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 41 0.0 0.0 0.0 0.0 0.0 42 0.0 0.0 0.0 0.0 0.0 43 0.0 0.0 0.0 0.0 0.0 44 0.0 0.0 0.0 0.0 0.0 45 0.0 0.0 0.0 0.0 0.0 46 0.0 0.0 0.0 0.0 0.0 47 0.0 0.0 0.0 0.0 0.0 48 0.0 0.0 0.0 0.0 0.0 49 0.0 0.0 0.0 0.0 0.0 50 0.0 0.0 0.0 0.0 0.0 51 0.0 0.0 0.0 0.0 0.0 52 0.0 0.0 0.0 0.0 0.0 53 0.0 0.0 0.0 0.0 0.0 54 0.0 0.0 0.0 0.0 0.0 55 0.0 0.0 0.0 0.0 0.0 56 0.0 0.0 0.0 0.0 0.0 57 0.0 0.0 0.0 0.0 0.0 58 0.0 0.0 0.0 0.0 0.0 59 0.0 0.0 0.0 0.0 0.0 60 0.0 0.0 0.0 0.0 0.0 61 0.0 0.0 0.0 0.0 0.0 62 0.0 0.0 0.0 0.0 0.0 63 0.0 0.0 0.0 0.0 0.0 64 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522154 spots for SRR9668903.sra Written 1522154 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra Read 1522143 spots for SRR9668903.sra Written 1522143 spots for SRR9668903.sra SRR ids: ['SRR9668903.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ma32bbmr SRR9668903.sra spots: 30442871 blocks: [[1, 1522143], [1522144, 3044286], [3044287, 4566429], [4566430, 6088572], [6088573, 7610715], [7610716, 9132858], [9132859, 10655001], [10655002, 12177144], [12177145, 13699287], [13699288, 15221430], [15221431, 16743573], [16743574, 18265716], [18265717, 19787859], [19787860, 21310002], [21310003, 22832145], [22832146, 24354288], [24354289, 25876431], [25876432, 27398574], [27398575, 28920717], [28920718, 30442871]] SRR9668903 file size 5774719 SRR9668903 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR9668903 SRR9668903_1.fastq SRR9668903_2.fastq Input file: SRR9668903_1.fastq Paired file: SRR9668903_2.fastq trimmed: SRR9668903-trimmed-pair1.fastq, SRR9668903-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 15:26:18 2025 >> started Wed Feb 12 15:27:00 2025 >> done (41.646s) 30442871 read pairs processed; of these: 7 ( 0.00%) short read pairs filtered out after trimming by size control 38001 ( 0.12%) empty read pairs filtered out after trimming by size control 30404863 (99.88%) read pairs available; of these: 3876 ( 0.01%) trimmed read pairs available after processing 30400987 (99.99%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 2 0.00% 20 4 0.00% 21 1 0.00% 22 3 0.00% 23 2 0.00% 24 7 0.00% 25 16 0.00% 26 5 0.00% 27 17 0.00% 28 12 0.00% 29 23 0.00% 30 35 0.00% 31 25 0.00% 32 21 0.00% 33 22 0.00% 34 28 0.00% 35 302 0.00% 36 343 0.00% 37 382 0.00% 38 514 0.00% 39 650 0.00% 40 741 0.00% 41 877 0.00% 42 1058 0.00% 43 1070 0.00% 44 1251 0.00% 45 1250 0.00% 46 1334 0.00% 47 1601 0.01% 48 1872 0.01% 49 2112 0.01% 50 2386 0.01% 51 2664 0.01% 52 3099 0.01% 53 3236 0.01% 54 3390 0.01% 55 3909 0.01% 56 4109 0.01% 57 4530 0.01% 58 4766 0.02% 59 5002 0.02% 60 6076 0.02% 61 6420 0.02% 62 6731 0.02% 63 7301 0.02% 64 7303 0.02% 65 7731 0.03% 66 8410 0.03% 67 8608 0.03% 68 8949 0.03% 69 9281 0.03% 70 11274 0.04% 71 17242 0.06% 72 30312 0.10% 73 193834 0.64% 74 2119768 6.97% 75 13841208 45.52% 76 14061744 46.25% 30404863 reads passed initial QC criterion=sequence-density sequence-density=1.66 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=22 prefix-density=1.54 prefix-fanout=2.0 sequence=CCGTCAATTCCTTT criterion=fanout-score sequence-density=0.20 sequence-density-rank=15 fanout-score=12.98 fanout-score-rank=1 prefix-density=1.45 prefix-fanout=1.8 sequence=CGCATCGCCGGCCCCCATCCACTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCT criterion=sequence-density sequence-density=0.83 sequence-density-rank=1 fanout-score=2.04 fanout-score-rank=22 prefix-density=0.86 prefix-fanout=2.0 sequence=ACGTGAGCTGGGTT criterion=fanout-score sequence-density=0.08 sequence-density-rank=22 fanout-score=21.89 fanout-score-rank=1 prefix-density=0.86 prefix-fanout=2.0 sequence=CGTGAGCTGGGATTAGACCGTCG SRR9668903 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 15:27:38 Started mapping on | Feb 12 15:27:39 Finished on | Feb 12 15:34:32 Mapping speed, Million of reads per hour | 265.03 Number of input reads | 30404863 Average input read length | 150 UNIQUE READS: Uniquely mapped reads number | 12401445 Uniquely mapped reads % | 40.79% Average mapped length | 149.63 Number of splices: Total | 3317297 Number of splices: Annotated (sjdb) | 3274991 Number of splices: GT/AG | 3251312 Number of splices: GC/AG | 51681 Number of splices: AT/AC | 2840 Number of splices: Non-canonical | 11464 Mismatch rate per base, % | 0.82% Deletion rate per base | 0.01% Deletion average length | 2.28 Insertion rate per base | 0.06% Insertion average length | 3.07 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1654493 % of reads mapped to multiple loci | 5.44% Number of reads mapped to too many loci | 11918222 % of reads mapped to too many loci | 39.20% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 13.08% % of reads unmapped: other | 1.50% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 16348925 16348925 16348925 N_multimapping 1654493 1654493 1654493 N_noFeature 3840479 11853833 3929964 N_ambiguous 515502 5038 53973 UnstrandedReadsAssigned:8045464 PositiveStrandReadsAssigned:542574 NegativeStrandReadsAssigned:8417508 Dataset is classified negative stranded MeadianReadLen=76 20thPercentileLength=75 echo kmer=71 SRR9668903 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR9668903-trimmed-pair1.fastq SRR9668903-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 30,404,863 reads, 16,607,515 reads pseudoaligned [quant] estimated average fragment length: 192.716 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 962 rounds 52401 SRR9668903.ke.tsv 34699 SRR9668903.se.tsv 87100 total ==> SRR9668903.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1826.28 148 2.5719 Potri.005G024800.1.v4.1 1035 843.284 42 1.58065 Potri.004G059700.1.v4.1 961 769.284 17 0.701332 Potri.007G009000.2.v4.1 1416 1224.28 0 0 Potri.003G141000.2.v4.1 2943 2751.28 113 1.30348 Potri.016G087400.1.v4.1 270 92.1033 509.072 175.415 Potri.015G069301.1.v4.1 564 372.396 0 0 Potri.010G195200.1.v4.1 1773 1581.28 2 0.0401404 Potri.012G127500.1.v4.1 977 785.284 2417 97.6813 ==> SRR9668903.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 12 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 104 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 1 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 19 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 4 SRR9668903 completed mapping pipeline successfully