Starting /dee2/code/volunteer_pipeline.sh SRR9668919 current disk space = 3051985551360 free memory = 1475656960 SRR9668919 SRAfilesize 224b587324bafba970df2a04e05dd8b4 SRR9668919.sra SRR9668919.sra file validated SRR9668919 is paired end SRR9668919 is conventional basespace SRR9668919 read1 length is 68-76 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR9668919_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 68-76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.44325 32.0 32.0 32.0 32.0 32.0 2 31.46325 32.0 32.0 32.0 32.0 32.0 3 31.51575 32.0 32.0 32.0 32.0 32.0 4 31.56825 32.0 32.0 32.0 32.0 32.0 5 31.57575 32.0 32.0 32.0 32.0 32.0 6 34.7205 36.0 36.0 36.0 36.0 36.0 7 35.18075 36.0 36.0 36.0 36.0 36.0 8 35.21325 36.0 36.0 36.0 36.0 36.0 9 35.2595 36.0 36.0 36.0 36.0 36.0 10-11 35.143875 36.0 36.0 36.0 36.0 36.0 12-13 35.140249999999995 36.0 36.0 36.0 36.0 36.0 14-15 35.086375000000004 36.0 36.0 36.0 36.0 36.0 16-17 35.095875 36.0 36.0 36.0 36.0 36.0 18-19 35.09975 36.0 36.0 36.0 36.0 36.0 20-21 35.058875 36.0 36.0 36.0 36.0 36.0 22-23 35.04375 36.0 36.0 36.0 36.0 36.0 24-25 34.94775 36.0 36.0 36.0 36.0 36.0 26-27 35.007999999999996 36.0 36.0 36.0 36.0 36.0 28-29 34.994125 36.0 36.0 36.0 36.0 36.0 30-31 34.945625 36.0 36.0 36.0 36.0 36.0 32-33 34.9195 36.0 36.0 36.0 34.0 36.0 34-35 34.819125 36.0 36.0 36.0 32.0 36.0 36-37 34.870125 36.0 36.0 36.0 36.0 36.0 38-39 34.908 36.0 36.0 36.0 34.0 36.0 40-41 34.853375 36.0 36.0 36.0 36.0 36.0 42-43 34.778875 36.0 36.0 36.0 36.0 36.0 44-45 34.675250000000005 36.0 36.0 36.0 32.0 36.0 46-47 34.790625000000006 36.0 36.0 36.0 32.0 36.0 48-49 34.739999999999995 36.0 36.0 36.0 32.0 36.0 50-51 34.68575 36.0 36.0 36.0 32.0 36.0 52-53 34.722125 36.0 36.0 36.0 32.0 36.0 54-55 34.643 36.0 36.0 36.0 32.0 36.0 56-57 34.509125 36.0 36.0 36.0 32.0 36.0 58-59 34.653 36.0 36.0 36.0 32.0 36.0 60-61 34.376125 36.0 36.0 36.0 32.0 36.0 62-63 34.312125 36.0 36.0 36.0 32.0 36.0 64-65 34.372375 36.0 36.0 36.0 32.0 36.0 66-67 34.36150000000001 36.0 36.0 36.0 32.0 36.0 68-69 34.25853769692423 36.0 36.0 36.0 32.0 36.0 70-71 34.19732281119305 36.0 36.0 36.0 32.0 36.0 72-73 34.23318057876882 36.0 36.0 36.0 32.0 36.0 74-75 34.16068729734833 36.0 36.0 36.0 32.0 36.0 76 33.22924187725632 36.0 32.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 1.0 23 4.0 24 4.0 25 9.0 26 12.0 27 36.0 28 52.0 29 71.0 30 90.0 31 122.0 32 158.0 33 277.0 34 610.0 35 2553.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.925000000000004 13.4 6.925000000000001 36.75 2 21.5 16.125 37.35 25.025 3 19.6 19.55 25.6 35.25 4 24.55 28.050000000000004 20.974999999999998 26.424999999999997 5 21.0 34.25 21.85 22.900000000000002 6 20.481012658227847 32.91139240506329 24.430379746835442 22.17721518987342 7 15.075 22.0 43.125 19.8 8 18.075 20.25 32.25 29.425 9 19.225 20.65 31.674999999999997 28.449999999999996 10-11 22.775000000000002 28.725 22.225 26.275 12-13 22.6 22.5875 26.387500000000003 28.425 14-15 21.9625 24.95 26.174999999999997 26.9125 16-17 23.7625 24.375 26.137500000000003 25.724999999999998 18-19 22.662499999999998 25.35 26.5 25.4875 20-21 22.237499999999997 25.2875 26.400000000000002 26.075 22-23 23.474999999999998 24.125 26.325 26.075 24-25 21.5375 24.4 26.625 27.437499999999996 26-27 22.075 24.725 25.687500000000004 27.5125 28-29 22.9375 24.75 24.5625 27.750000000000004 30-31 22.875 25.0 24.9125 27.212500000000002 32-33 22.5 24.5375 26.6125 26.35 34-35 22.3 25.074999999999996 26.0 26.625 36-37 21.725 25.937500000000004 26.1 26.237500000000004 38-39 22.0 25.2125 25.2625 27.525 40-41 23.225 25.124999999999996 24.775 26.875 42-43 23.1375 23.4875 27.625 25.75 44-45 22.125 24.962500000000002 27.1125 25.8 46-47 23.925 24.587500000000002 26.150000000000002 25.337500000000002 48-49 22.412499999999998 24.3 25.937500000000004 27.35 50-51 21.875 25.074999999999996 25.874999999999996 27.175 52-53 21.925 25.525 26.8625 25.687500000000004 54-55 21.7375 26.724999999999998 25.75 25.7875 56-57 21.5625 24.1125 26.987499999999997 27.3375 58-59 21.212500000000002 24.875 26.924999999999997 26.987499999999997 60-61 22.35 24.5125 26.2625 26.875 62-63 22.175 23.8625 27.212500000000002 26.75 64-65 21.6625 23.9125 26.700000000000003 27.725 66-67 22.2125 25.362499999999997 25.412499999999998 27.0125 68-69 21.027628453556694 25.753219152394045 26.003250406300786 27.21590198774847 70-71 22.633487557834187 24.359134675503313 25.447042640990368 27.560335125672125 72-73 22.93704539754201 24.968648106345622 25.232004013042385 26.86230248306998 74-75 22.325764536028352 22.955768473552958 26.053287833048955 28.665179157369735 76 24.69314079422383 0.0 37.03971119133574 38.26714801444043 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.5 21 1.0 22 1.0 23 3.0 24 4.0 25 4.0 26 6.0 27 8.0 28 9.5 29 11.5 30 18.0 31 25.0 32 31.5 33 43.5 34 47.0 35 58.0 36 88.0 37 98.5 38 97.0 39 106.5 40 115.0 41 148.5 42 173.0 43 174.5 44 188.5 45 223.5 46 262.5 47 257.0 48 233.5 49 264.5 50 284.5 51 250.0 52 227.5 53 208.5 54 205.0 55 215.0 56 201.0 57 173.5 58 173.5 59 170.0 60 139.0 61 94.5 62 62.0 63 46.0 64 29.5 65 23.0 66 21.0 67 20.5 68 19.0 69 13.5 70 10.5 71 10.0 72 13.5 73 16.0 74 9.0 75 3.5 76 2.0 77 1.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.25 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 68 1.0 69 0.0 70 1.0 71 2.0 72 18.0 73 56.0 74 225.0 75 927.0 76 2770.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.52499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 90.09168884945282 76.14999999999999 2 6.122448979591836 10.35 3 1.8041999408459037 4.575 4 0.8281573498964804 2.8000000000000003 5 0.5915409642117717 2.5 6 0.3253475303164744 1.6500000000000001 7 0.05915409642117716 0.35000000000000003 8 0.0 0.0 9 0.0 0.0 >10 0.1774622892635315 1.625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCAT 12 0.3 No Hit CGGCAATCGGACGGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGACT 11 0.27499999999999997 No Hit GTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGC 11 0.27499999999999997 No Hit GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGGACTTCTCGCG 11 0.27499999999999997 No Hit GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTCCACC 10 0.25 No Hit GTCGAGTTATCATGAATCATCAGAGCAACGGGCAGAGCCCGCGTCGACCTTTTATCTAATAAATGCGTCCCTTCC 10 0.25 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAACCCA 7 0.17500000000000002 No Hit GTCGGCAATCGGACGGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCA 7 0.17500000000000002 No Hit GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG 6 0.15 No Hit AGAAATTTGAATGATGCGTCGCCAGCACGAAGGCCGTGCGATCCGTCGAG 6 0.15 No Hit GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG 6 0.15 No Hit GGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTCTTTCGC 6 0.15 No Hit ATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGCTATAG 6 0.15 No Hit CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA 6 0.15 No Hit GTCGGTTCGGACCTCCACTTAGTTTCACCCAAGCTTCATCCTGGTCATGGATAGATCACCCAGGTTCGGGTCCAT 6 0.15 No Hit CGATAGAACTCGCACCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATT 6 0.15 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTACAGCACTGC 6 0.15 No Hit CAACAACTTAAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGC 6 0.15 No Hit CGGACGGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAACGCACGGT 6 0.15 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 5 0.125 No Hit CGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGGACTTCTCGCGACGTCGCCGGCGGCG 5 0.125 No Hit GCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAG 5 0.125 No Hit CTTTTATCTAATAAATGCGTCCCTTCCAGAAGTCGGGGTTTGTTGCACGT 5 0.125 No Hit GCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCACCGGAGGCACG 5 0.125 No Hit CTCGCACCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT 5 0.125 No Hit CGCCAATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTG 5 0.125 No Hit ACCATCTTTCGGGTCCCGACAGGCATGCTCTCACTCGAACCCTTCTCAGA 5 0.125 No Hit GCCGACCTTGACCCCTGTTATTTTGAGGTCATATCTAGTATTCAGAGTTT 5 0.125 No Hit GTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCA 5 0.125 No Hit CCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG 5 0.125 No Hit GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCC 5 0.125 No Hit CTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACAC 5 0.125 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 5 0.125 No Hit GGCACGTGCCTCCGGGGCCAAGAGGCCCCTACTGCAGGTCGGCAATCGGA 5 0.125 No Hit CTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTT 5 0.125 No Hit CCAGAACCCAAAAACTTTGATTTCTCATAAGGTGCTGGCGGAGTCCTAAA 5 0.125 No Hit GTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCT 5 0.125 No Hit CTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAA 5 0.125 No Hit CGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 41 0.0 0.0 0.0 0.0 0.0 42 0.0 0.0 0.0 0.0 0.0 43 0.0 0.0 0.0 0.0 0.0 44 0.0 0.0 0.0 0.0 0.0 45 0.0 0.0 0.0 0.0 0.0 46 0.0 0.0 0.0 0.0 0.0 47 0.0 0.0 0.0 0.0 0.0 48 0.0 0.0 0.0 0.0 0.0 49 0.0 0.0 0.0 0.0 0.0 50 0.0 0.0 0.0 0.0 0.0 51 0.0 0.0 0.0 0.0 0.0 52 0.0 0.0 0.0 0.0 0.0 53 0.0 0.0 0.0 0.0 0.0 54 0.0 0.0 0.0 0.0 0.0 55 0.0 0.0 0.0 0.0 0.0 56 0.0 0.0 0.0 0.0 0.0 57 0.0 0.0 0.0 0.0 0.0 58 0.0 0.0 0.0 0.0 0.0 59 0.0 0.0 0.0 0.0 0.0 60 0.0 0.0 0.0 0.0 0.0 61 0.0 0.0 0.0 0.0 0.0 62 0.0 0.0 0.0 0.0 0.0 63 0.0 0.0 0.0 0.0 0.0 64 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAAGCA 20 0.006668267 52.078125 48 >>END_MODULE SRR9668919 read2 length is 35-76 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR9668919_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 35-76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.13625 32.0 32.0 32.0 32.0 32.0 2 30.90125 32.0 32.0 32.0 32.0 32.0 3 30.8915 32.0 32.0 32.0 32.0 32.0 4 30.87225 32.0 32.0 32.0 32.0 32.0 5 30.88925 32.0 32.0 32.0 32.0 32.0 6 34.2965 36.0 36.0 36.0 32.0 36.0 7 34.544 36.0 36.0 36.0 32.0 36.0 8 34.24875 36.0 36.0 36.0 32.0 36.0 9 34.53 36.0 36.0 36.0 32.0 36.0 10-11 34.31625 36.0 36.0 36.0 32.0 36.0 12-13 34.372 36.0 36.0 36.0 32.0 36.0 14-15 34.356750000000005 36.0 36.0 36.0 32.0 36.0 16-17 34.241875 36.0 36.0 36.0 32.0 36.0 18-19 34.348124999999996 36.0 36.0 36.0 32.0 36.0 20-21 34.27775 36.0 36.0 36.0 32.0 36.0 22-23 34.171875 36.0 36.0 36.0 32.0 36.0 24-25 34.265375 36.0 36.0 36.0 32.0 36.0 26-27 34.149375 36.0 36.0 36.0 32.0 36.0 28-29 34.045625 36.0 36.0 36.0 32.0 36.0 30-31 34.056125 36.0 36.0 36.0 32.0 36.0 32-33 34.127624999999995 36.0 36.0 36.0 32.0 36.0 34-35 33.945125000000004 36.0 36.0 36.0 32.0 36.0 36-37 34.07325319308791 36.0 36.0 36.0 32.0 36.0 38-39 33.953551166946966 36.0 36.0 36.0 29.5 36.0 40-41 33.93411823647294 36.0 36.0 36.0 32.0 36.0 42-43 33.727079158316634 36.0 36.0 36.0 27.0 36.0 44-45 33.79131509411723 36.0 36.0 36.0 29.5 36.0 46-47 33.79676529588767 36.0 36.0 36.0 27.0 36.0 48-49 34.02094831911691 36.0 36.0 36.0 32.0 36.0 50-51 33.907927747114904 36.0 36.0 36.0 29.5 36.0 52-53 33.73156046161566 36.0 36.0 36.0 32.0 36.0 54-55 33.69242348218766 36.0 36.0 36.0 29.5 36.0 56-57 33.63534872052183 36.0 36.0 36.0 27.0 36.0 58-59 33.47102358253889 36.0 36.0 36.0 27.0 36.0 60-61 33.6957819432002 36.0 36.0 36.0 27.0 36.0 62-63 33.70401505646173 36.0 36.0 36.0 27.0 36.0 64-65 33.478288152610446 36.0 36.0 36.0 27.0 36.0 66-67 33.41747428453457 36.0 36.0 36.0 27.0 36.0 68-69 33.49771178892202 36.0 36.0 36.0 27.0 36.0 70-71 33.59805574744797 36.0 36.0 36.0 27.0 36.0 72-73 33.530695604781634 36.0 36.0 36.0 27.0 36.0 74-75 33.54638688925884 36.0 36.0 36.0 27.0 36.0 76 32.55963650132526 36.0 32.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 2.0 15 10.0 16 11.0 17 5.0 18 11.0 19 6.0 20 13.0 21 11.0 22 10.0 23 13.0 24 18.0 25 36.0 26 56.0 27 53.0 28 80.0 29 96.0 30 102.0 31 166.0 32 177.0 33 305.0 34 669.0 35 2142.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.869346733668344 21.13065326633166 9.924623115577889 25.075376884422113 2 32.731376975169304 23.5766240280913 29.29520943064961 14.396789566089794 3 25.526052104208418 25.626252505010022 25.851703406813627 22.995991983967937 4 28.09917355371901 33.78412221387428 19.383921863260706 18.732782369146005 5 30.628600050087652 34.234911094415224 16.979714500375657 18.156774355121463 6 25.093914350112694 36.5389431505134 18.90808915602304 19.459053343350863 7 24.868519909842224 18.357124968695217 36.01302278988229 20.761332331580267 8 24.142248935637365 22.188830453293264 26.3961933383421 27.27272727272727 9 24.718256949661907 23.240671174555473 27.87377911344853 24.167292762334082 10-11 27.89882294014525 29.138492361632856 20.485850237916353 22.476834460305533 12-13 28.737791134485352 23.40345604808415 24.455296769346358 23.40345604808415 14-15 26.189879759519037 26.50300601202405 25.288076152304612 22.019038076152306 16-17 27.25222403207618 26.024307730860798 24.182433279037717 22.54103495802531 18-19 27.56763527054108 26.114729458917836 23.471943887775552 22.84569138276553 20-21 27.467434869739478 26.866232464929862 23.772545090180362 21.8937875751503 22-23 28.07171514543631 26.06569709127382 23.470411233701103 22.392176529588767 24-25 27.520982086934737 25.541776274583487 24.41438055868721 22.522861079794563 26-27 26.415330661322646 26.84118236472946 24.423847695390783 22.319639278557112 28-29 27.00501253132832 26.81704260651629 23.50877192982456 22.669172932330827 30-31 28.324134470647266 25.70245860511791 23.469643753135976 22.503763171098846 32-33 26.910548734652966 26.45953395139063 24.417439238286143 22.21247807567026 34-35 26.85220007521625 27.127992979816973 23.93130249467218 22.088504450294597 36-37 27.102686417273414 26.638212402711524 23.68817474265629 22.570926437358775 38-39 26.58036948598718 27.070503958778435 24.51929119014704 21.829835365087344 40-41 27.617010568696525 26.57272269753397 23.414695520885758 22.395571212883745 42-43 26.945353815159912 26.769075799546716 24.263409720473433 22.022160664819946 44-45 27.47668263171162 25.22056970002521 24.89286614570204 22.40988152256113 46-47 26.955535961708023 26.653230885501955 24.159214006801864 22.23201914598816 48-49 27.89850521291295 26.31578947368421 22.848888330611732 22.936816982791104 50-51 26.76463200200955 27.304697312233106 24.767646320020095 21.16302436573725 52-53 27.249874308697837 25.1131221719457 25.087983911513323 22.54901960784314 54-55 27.085167945653545 26.456158007296516 23.738835073594164 22.71983897345578 56-57 26.961339881627 25.299080720312304 25.248709230575493 22.490870167485202 58-59 27.828823159219635 26.04153555695406 24.921334172435493 21.208307111390813 60-61 26.845806127574086 26.205424409844298 24.886991461577097 22.06177800100452 62-63 27.516019600452317 24.814675210453572 24.412614650081668 23.25669053901244 64-65 27.922486472882852 26.14823203724676 23.94614319869133 21.98313829117906 66-67 27.6274386406545 25.663939584644428 24.06544996853367 22.643171806167402 68-69 27.361932318530634 25.223298528116743 24.242043024279784 23.172726129072842 70-71 27.149321266968325 25.163398692810457 25.050276520864756 22.637003519356462 72-73 28.207713637509453 26.191076380136124 23.35518023695488 22.246029745399547 74-75 27.408503767491926 22.349300322927878 25.645855758880515 24.596340150699678 76 31.174242424242426 0.0 35.60606060606061 33.21969696969697 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7.0 1 3.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 1.0 12 2.0 13 1.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 1.0 20 0.0 21 0.0 22 0.0 23 0.5 24 1.5 25 3.0 26 5.0 27 7.5 28 11.0 29 11.0 30 13.0 31 18.5 32 24.0 33 25.0 34 35.0 35 55.0 36 72.5 37 86.0 38 95.5 39 119.5 40 141.0 41 165.0 42 174.0 43 203.5 44 252.0 45 235.5 46 223.0 47 257.5 48 264.5 49 239.0 50 230.5 51 215.0 52 196.0 53 190.5 54 185.5 55 187.0 56 177.0 57 166.0 58 158.0 59 149.5 60 143.0 61 117.5 62 96.5 63 71.0 64 48.5 65 38.5 66 22.0 67 18.0 68 26.0 69 27.5 70 15.0 71 6.0 72 9.5 73 12.5 74 10.0 75 7.0 76 4.0 77 5.0 78 5.5 79 3.0 80 1.5 81 1.5 82 2.5 83 2.0 84 1.0 85 1.0 86 1.0 87 0.5 88 1.5 89 2.0 90 1.0 91 0.5 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.5 98 1.0 99 6.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5 2 0.325 3 0.2 4 0.17500000000000002 5 0.17500000000000002 6 0.17500000000000002 7 0.17500000000000002 8 0.17500000000000002 9 0.17500000000000002 10-11 0.17500000000000002 12-13 0.17500000000000002 14-15 0.2 16-17 0.2375 18-19 0.2 20-21 0.2 22-23 0.3 24-25 0.21250000000000002 26-27 0.2 28-29 0.25 30-31 0.35000000000000003 32-33 0.22499999999999998 34-35 0.2875 36-37 0.25043826696719257 38-39 0.3506574827802129 40-41 0.45090180360721444 42-43 0.5260521042084169 44-45 0.5639804486777792 46-47 0.4638916750250752 48-49 0.13798294029101857 50-51 0.1254390366281987 52-53 0.2007024586051179 54-55 0.288509784244857 56-57 0.3888610135474159 58-59 0.33868539889613647 60-61 0.08781834148789361 62-63 0.13801756587202008 64-65 0.2635542168674699 66-67 0.2761390736789256 68-69 0.17581313575285698 70-71 0.03769317753486619 72-73 0.08815010703941568 74-75 0.026903416733925208 76 0.03786444528587656 >>END_MODULE >>Sequence Length Distribution warn #Length Count 35 7.0 36 0.0 37 0.0 38 1.0 39 0.0 40 0.0 41 0.0 42 0.0 43 1.0 44 3.0 45 0.0 46 0.0 47 2.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 1.0 61 0.0 62 0.0 63 1.0 64 0.0 65 0.0 66 1.0 67 0.0 68 3.0 69 0.0 70 1.0 71 1.0 72 15.0 73 89.0 74 314.0 75 919.0 76 2641.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.225 #Duplication Level Percentage of deduplicated Percentage of total 1 90.91430209229006 79.3 2 6.534823731728288 11.4 3 1.5763829177414732 4.125 4 0.40126110633419315 1.4000000000000001 5 0.2579535683576956 1.125 6 0.08598452278589853 0.44999999999999996 7 0.028661507595299514 0.17500000000000002 8 0.028661507595299514 0.2 9 0.0 0.0 >10 0.17196904557179707 1.825 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTAGTTTTACCCTACTGATGACAGTGTCGCAATAGTAATCCAACCTAGTACGAGAGGAACCGTTGATTCGCACA 15 0.375 No Hit GTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCAAGGAAGCTGACTGGCGGGATCCCCTCG 15 0.375 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 12 0.3 No Hit GGAGTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCA 11 0.27499999999999997 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAA 10 0.25 No Hit CTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCAAGGAA 10 0.25 No Hit GTTTGTTTGATGGTATTTGCTACTCGGATAACCGTAGTAATTCTAGAGCT 8 0.2 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 7 0.17500000000000002 No Hit CTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTT 6 0.15 No Hit ACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 6 0.15 No Hit CTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCG 6 0.15 No Hit CTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACTAATTCAGACTGTGAAACTGCGAATGGCTCATTAA 5 0.125 No Hit CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT 5 0.125 No Hit CTCAGGATAGCTGGAGCTCGGTGCGAGTTCTATCGGGTAAAGCCAATGAT 5 0.125 No Hit CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAG 5 0.125 No Hit GGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTC 5 0.125 No Hit GGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAA 5 0.125 No Hit AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG 5 0.125 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAA 5 0.125 No Hit GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 41 0.0 0.0 0.0 0.0 0.0 42 0.0 0.0 0.0 0.0 0.0 43 0.0 0.0 0.0 0.0 0.0 44 0.0 0.0 0.0 0.0 0.0 45 0.0 0.0 0.0 0.0 0.0 46 0.0 0.0 0.0 0.0 0.0 47 0.0 0.0 0.0 0.0 0.0 48 0.0 0.0 0.0 0.0 0.0 49 0.0 0.0 0.0 0.0 0.0 50 0.0 0.0 0.0 0.0 0.0 51 0.0 0.0 0.0 0.0 0.0 52 0.0 0.0 0.0 0.0 0.0 53 0.0 0.0 0.0 0.0 0.0 54 0.0 0.0 0.0 0.0 0.0 55 0.0 0.0 0.0 0.0 0.0 56 0.0 0.0 0.0 0.0 0.0 57 0.0 0.0 0.0 0.0 0.0 58 0.0 0.0 0.0 0.0 0.0 59 0.0 0.0 0.0 0.0 0.0 60 0.0 0.0 0.0 0.0 0.0 61 0.0 0.0 0.0 0.0 0.0 62 0.0 0.0 0.0 0.0 0.0 63 0.0 0.0 0.0 0.0 0.0 64 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1868413 spots for SRR9668919.sra Written 1868413 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra Read 1868405 spots for SRR9668919.sra Written 1868405 spots for SRR9668919.sra SRR ids: ['SRR9668919.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_tmbivw2s SRR9668919.sra spots: 37368108 blocks: [[1, 1868405], [1868406, 3736810], [3736811, 5605215], [5605216, 7473620], [7473621, 9342025], [9342026, 11210430], [11210431, 13078835], [13078836, 14947240], [14947241, 16815645], [16815646, 18684050], [18684051, 20552455], [20552456, 22420860], [22420861, 24289265], [24289266, 26157670], [26157671, 28026075], [28026076, 29894480], [29894481, 31762885], [31762886, 33631290], [33631291, 35499695], [35499696, 37368108]] SRR9668919 file size 7097896 SRR9668919 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR9668919 SRR9668919_1.fastq SRR9668919_2.fastq Input file: SRR9668919_1.fastq Paired file: SRR9668919_2.fastq trimmed: SRR9668919-trimmed-pair1.fastq, SRR9668919-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 16:35:35 2025 >> started Wed Feb 12 16:36:25 2025 >> done (49.851s) 37368108 read pairs processed; of these: 37 ( 0.00%) short read pairs filtered out after trimming by size control 5253 ( 0.01%) empty read pairs filtered out after trimming by size control 37362818 (99.99%) read pairs available; of these: 4891 ( 0.01%) trimmed read pairs available after processing 37357927 (99.99%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 2 0.00% 20 6 0.00% 21 3 0.00% 22 4 0.00% 23 7 0.00% 24 8 0.00% 25 5 0.00% 26 10 0.00% 27 8 0.00% 28 16 0.00% 29 21 0.00% 30 13 0.00% 31 11 0.00% 32 12 0.00% 33 15 0.00% 34 19 0.00% 35 122 0.00% 36 148 0.00% 37 169 0.00% 38 184 0.00% 39 188 0.00% 40 206 0.00% 41 235 0.00% 42 261 0.00% 43 275 0.00% 44 356 0.00% 45 309 0.00% 46 268 0.00% 47 380 0.00% 48 436 0.00% 49 561 0.00% 50 640 0.00% 51 806 0.00% 52 823 0.00% 53 818 0.00% 54 963 0.00% 55 1453 0.00% 56 1344 0.00% 57 1331 0.00% 58 1511 0.00% 59 1687 0.00% 60 2049 0.01% 61 2173 0.01% 62 2364 0.01% 63 2663 0.01% 64 2854 0.01% 65 3218 0.01% 66 3593 0.01% 67 4042 0.01% 68 4113 0.01% 69 4612 0.01% 70 6345 0.02% 71 12058 0.03% 72 26935 0.07% 73 252233 0.68% 74 2696325 7.22% 75 17259477 46.19% 76 17062130 45.67% 37362818 reads passed initial QC criterion=sequence-density sequence-density=1.19 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=26 prefix-density=1.12 prefix-fanout=2.0 sequence=CCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAACCCAAAAACTTTGATTTCTCATAAGGTGCTGGCGGAGTCCTAAAAGCAACATCCGCCAATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATCCAAGAATTTCACCTCTGACTATGAAATACGAATGCCCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCACCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCGCCG criterion=fanout-score sequence-density=0.20 sequence-density-rank=14 fanout-score=12.49 fanout-score-rank=1 prefix-density=1.07 prefix-fanout=2.4 sequence=CGCATCGCCGGTAGAAGGGACGAGGCGACCGGTGCACACCTGAGGCGGACCGGCCGACCCAACCCAAAGTCCAACTACGAGCTTTTTAACTGCAACAACTTAAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAGGGATTTAGATTGTACTCATTCCAATTACCAGACTCGAAGAGCCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCTCTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGATGCGTCGCCAGCACGAAGGCCGTGCGATCCGTCGAGTTATCATGAATCATCAGAGCAACGGGCAGAGCCCGCGTCGACCTTTTATCTAATAAATGCGTC criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=3.40 fanout-score-rank=15 prefix-density=0.57 prefix-fanout=1.9 sequence=TCGTGAGACAGGTTAGTTTTACCCTACTGATGACAGTGTCGCAATAGTAATCCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTACCGTGCGTTGGATTATGACTGAACGCCTCTAAGTCAGAATCCGGGCTAGATGCGACGCGTGCGCCCGCCGTCCGATTGCCGACCTGCAGTAGGGGCCTCTTGGCCCCGGAGGCACGTGCCGTTGGCCAAGCCCTCGCGGTGAAAGAGCCGCGCGGGCCGCCTTGAAGTACAATTCCCACCGAGCGGCGGGTAGAATCCTTTGCAGACGACTTAAATACGCGACGGGGTATTGTA criterion=fanout-score sequence-density=0.02 sequence-density-rank=32 fanout-score=22.76 fanout-score-rank=1 prefix-density=0.22 prefix-fanout=2.0 sequence=GGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAAGAGACGGGGGAAGCCCGTCCGACAGCGCGTTCGCGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTCCTGAACCGGGACGTGGCGGCTGACGGCAACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCCGCCCACCCTGGAAACGACTTAGTCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCGCACGTCGCGTGGTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCTCCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGG SRR9668919 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 16:36:52 Started mapping on | Feb 12 16:36:52 Finished on | Feb 12 16:46:08 Mapping speed, Million of reads per hour | 241.92 Number of input reads | 37362818 Average input read length | 151 UNIQUE READS: Uniquely mapped reads number | 21033316 Uniquely mapped reads % | 56.29% Average mapped length | 150.16 Number of splices: Total | 7261362 Number of splices: Annotated (sjdb) | 7175089 Number of splices: GT/AG | 7126568 Number of splices: GC/AG | 112914 Number of splices: AT/AC | 5660 Number of splices: Non-canonical | 16220 Mismatch rate per base, % | 0.70% Deletion rate per base | 0.02% Deletion average length | 2.19 Insertion rate per base | 0.03% Insertion average length | 2.62 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1981609 % of reads mapped to multiple loci | 5.30% Number of reads mapped to too many loci | 11940644 % of reads mapped to too many loci | 31.96% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.81% % of reads unmapped: other | 1.63% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 14347936 14347936 14347936 N_multimapping 1981609 1981609 1981609 N_noFeature 3751083 20479295 3863182 N_ambiguous 552279 4294 106568 UnstrandedReadsAssigned:16729954 PositiveStrandReadsAssigned:549727 NegativeStrandReadsAssigned:17063566 Dataset is classified negative stranded MeadianReadLen=76 20thPercentileLength=75 echo kmer=71 SRR9668919 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR9668919-trimmed-pair1.fastq SRR9668919-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 37,362,818 reads, 25,528,489 reads pseudoaligned [quant] estimated average fragment length: 193.254 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,034 rounds 52401 SRR9668919.ke.tsv 34699 SRR9668919.se.tsv 87100 total ==> SRR9668919.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1825.75 339 4.58587 Potri.005G024800.1.v4.1 1035 842.746 72 2.11008 Potri.004G059700.1.v4.1 961 768.754 41 1.31722 Potri.007G009000.2.v4.1 1416 1223.75 0 0 Potri.003G141000.2.v4.1 2943 2750.75 319 2.86419 Potri.016G087400.1.v4.1 270 92.7251 1119.88 298.288 Potri.015G069301.1.v4.1 564 371.87 0 0 Potri.010G195200.1.v4.1 1773 1580.75 7 0.10937 Potri.012G127500.1.v4.1 977 784.754 4722 148.612 ==> SRR9668919.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 17 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 288 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 1 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 85 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 7 SRR9668919 completed mapping pipeline successfully