Starting /dee2/code/volunteer_pipeline.sh SRR9668923 current disk space = 3051957276672 free memory = 1442986060 SRR9668923 SRAfilesize 46ae1ad5305ece9c392f403aaaaef332 SRR9668923.sra SRR9668923.sra file validated SRR9668923 is paired end SRR9668923 is conventional basespace SRR9668923 read1 length is 57-76 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR9668923_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 57-76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.502 32.0 32.0 32.0 32.0 32.0 2 31.4995 32.0 32.0 32.0 32.0 32.0 3 31.53925 32.0 32.0 32.0 32.0 32.0 4 31.5615 32.0 32.0 32.0 32.0 32.0 5 31.57975 32.0 32.0 32.0 32.0 32.0 6 34.6705 36.0 36.0 36.0 36.0 36.0 7 35.073 36.0 36.0 36.0 36.0 36.0 8 35.1425 36.0 36.0 36.0 36.0 36.0 9 35.13425 36.0 36.0 36.0 36.0 36.0 10-11 35.112750000000005 36.0 36.0 36.0 36.0 36.0 12-13 35.0815 36.0 36.0 36.0 36.0 36.0 14-15 35.05575 36.0 36.0 36.0 36.0 36.0 16-17 35.087875 36.0 36.0 36.0 36.0 36.0 18-19 35.12425 36.0 36.0 36.0 36.0 36.0 20-21 35.05 36.0 36.0 36.0 36.0 36.0 22-23 35.029625 36.0 36.0 36.0 36.0 36.0 24-25 34.928749999999994 36.0 36.0 36.0 34.0 36.0 26-27 34.90475 36.0 36.0 36.0 36.0 36.0 28-29 34.926375 36.0 36.0 36.0 34.0 36.0 30-31 34.901125 36.0 36.0 36.0 36.0 36.0 32-33 34.875875 36.0 36.0 36.0 32.0 36.0 34-35 34.844625 36.0 36.0 36.0 32.0 36.0 36-37 34.806375 36.0 36.0 36.0 34.0 36.0 38-39 34.7905 36.0 36.0 36.0 32.0 36.0 40-41 34.683 36.0 36.0 36.0 32.0 36.0 42-43 34.71325 36.0 36.0 36.0 34.0 36.0 44-45 34.6545 36.0 36.0 36.0 32.0 36.0 46-47 34.8035 36.0 36.0 36.0 32.0 36.0 48-49 34.653125 36.0 36.0 36.0 32.0 36.0 50-51 34.61725 36.0 36.0 36.0 32.0 36.0 52-53 34.554 36.0 36.0 36.0 32.0 36.0 54-55 34.6515 36.0 36.0 36.0 32.0 36.0 56-57 34.442375 36.0 36.0 36.0 32.0 36.0 58-59 34.58152038009503 36.0 36.0 36.0 32.0 36.0 60-61 34.42635658914729 36.0 36.0 36.0 32.0 36.0 62-63 34.21992998249563 36.0 36.0 36.0 32.0 36.0 64-65 34.270567641910475 36.0 36.0 36.0 32.0 36.0 66-67 34.344663786662196 36.0 36.0 36.0 32.0 36.0 68-69 34.0787037037037 36.0 36.0 36.0 32.0 36.0 70-71 34.03491720757053 36.0 36.0 36.0 32.0 36.0 72-73 34.201195133758866 36.0 36.0 36.0 32.0 36.0 74-75 34.14261211891847 36.0 36.0 36.0 32.0 36.0 76 33.36449275362319 36.0 32.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 1.0 24 6.0 25 13.0 26 23.0 27 42.0 28 48.0 29 71.0 30 84.0 31 130.0 32 181.0 33 258.0 34 666.0 35 2476.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.074999999999996 11.225 9.45 46.25 2 21.65 15.525 37.45 25.374999999999996 3 18.725 18.55 25.874999999999996 36.85 4 22.975 28.125 21.275 27.625 5 23.75 30.775000000000002 23.724999999999998 21.75 6 20.035505959928987 30.96626933806746 25.38676134922648 23.611463352777072 7 15.575 19.575 43.075 21.775 8 18.4 19.275000000000002 31.85 30.475 9 20.125 19.225 32.0 28.65 10-11 22.9875 28.5875 20.9 27.525 12-13 23.1125 21.5375 27.450000000000003 27.900000000000002 14-15 22.025 23.400000000000002 27.3375 27.237499999999997 16-17 23.3625 23.849999999999998 25.224999999999998 27.5625 18-19 22.162499999999998 23.8125 25.587500000000002 28.4375 20-21 22.6875 23.6125 26.674999999999997 27.025 22-23 22.4875 24.275 25.0625 28.175 24-25 23.025000000000002 24.725 24.8625 27.3875 26-27 22.662499999999998 23.5375 25.912499999999998 27.8875 28-29 22.787499999999998 23.3 25.662499999999998 28.249999999999996 30-31 21.75 23.3 26.75 28.199999999999996 32-33 22.3875 23.674999999999997 25.412499999999998 28.525 34-35 21.587500000000002 24.775 26.375 27.2625 36-37 22.3 24.9125 25.5 27.287499999999998 38-39 22.525000000000002 23.275000000000002 25.887500000000003 28.3125 40-41 22.825 24.525 24.9875 27.6625 42-43 23.7125 24.087500000000002 26.1125 26.087500000000002 44-45 22.537499999999998 23.599999999999998 27.0125 26.85 46-47 23.7875 22.475 26.674999999999997 27.0625 48-49 22.425 22.875 26.337500000000002 28.3625 50-51 21.8625 23.925 26.275 27.9375 52-53 22.7125 24.025 26.474999999999998 26.787499999999998 54-55 22.8625 24.55 25.162499999999998 27.425 56-57 20.525 24.4875 26.924999999999997 28.0625 58-59 22.20555138784696 23.48087021755439 26.731682920730183 27.581895473868467 60-61 22.268067016754188 23.36834208552138 26.294073518379594 28.069517379344838 62-63 23.80595148787197 23.568392098024507 26.431607901975497 26.19404851212803 64-65 21.880470117529384 22.31807951987997 26.70667666916729 29.094773693423353 66-67 21.898449224612307 24.72486243121561 25.65032516258129 27.726363181590795 68-69 22.32232232232232 23.723723723723726 26.526526526526528 27.427427427427425 70-71 22.98836190714554 24.164685270929795 25.140783381303965 27.706169440620698 72-73 21.76477971632986 23.43416593447973 26.170453119116356 28.630601230074053 74-75 21.950574864543412 20.60261662481829 26.483414827540635 30.963393683097664 76 26.268115942028984 0.0 37.210144927536234 36.52173913043478 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.0 19 1.5 20 2.0 21 1.0 22 1.0 23 3.5 24 3.5 25 2.0 26 6.5 27 10.5 28 11.0 29 11.0 30 17.5 31 22.5 32 25.0 33 33.5 34 40.5 35 45.5 36 57.0 37 68.5 38 71.5 39 74.0 40 84.5 41 129.0 42 171.5 43 179.0 44 178.0 45 201.5 46 224.0 47 228.0 48 231.5 49 245.0 50 257.0 51 243.5 52 237.0 53 224.5 54 217.0 55 238.5 56 242.5 57 204.5 58 171.0 59 172.5 60 177.5 61 124.0 62 73.0 63 64.5 64 43.0 65 27.0 66 24.0 67 25.0 68 21.0 69 17.0 70 16.0 71 14.5 72 21.0 73 24.5 74 14.5 75 7.5 76 3.5 77 1.0 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.425 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 57 1.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 2.0 67 1.0 68 0.0 69 0.0 70 1.0 71 2.0 72 19.0 73 70.0 74 241.0 75 903.0 76 2760.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.675 #Duplication Level Percentage of deduplicated Percentage of total 1 88.27670645852464 72.1 2 7.07070707070707 11.55 3 2.509947964493419 6.15 4 1.0101010101010102 3.3000000000000003 5 0.39791857973676154 1.625 6 0.15304560759106214 0.75 7 0.21426385062748698 1.225 8 0.12243648607284971 0.8 9 0.061218243036424855 0.44999999999999996 >10 0.18365472910927455 2.0500000000000003 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGGCAATCGGACGGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGACT 19 0.475 No Hit GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTCCACC 18 0.44999999999999996 No Hit GTCAGTATCGCTGCGGGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGC 14 0.35000000000000003 No Hit GGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTCTTTCGC 11 0.27499999999999997 No Hit GACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 10 0.25 No Hit GATAGAACTCGCACCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGA 10 0.25 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 9 0.22499999999999998 No Hit GCTTTACCCGATAGAACTCGCACCGAGCTCCAGCTATCCTGAGGGAAACT 9 0.22499999999999998 No Hit CTCCTACTCATCGGGGCCTGGCGCTTGCCCCGACGGCCGGGTATAGGTCG 8 0.2 No Hit CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCC 8 0.2 No Hit CTTGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGA 8 0.2 No Hit CCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGCTCTCGATTCCGTGGCGCGGCTCAACGAAGC 8 0.2 No Hit CGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTT 7 0.17500000000000002 No Hit CTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCAACAGGGTCTTC 7 0.17500000000000002 No Hit GTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCA 7 0.17500000000000002 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 7 0.17500000000000002 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAACCCA 7 0.17500000000000002 No Hit CCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGG 7 0.17500000000000002 No Hit CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTT 7 0.17500000000000002 No Hit CCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGC 6 0.15 No Hit CTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGAC 6 0.15 No Hit CGATAGAACTCGCACCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATT 6 0.15 No Hit GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATC 6 0.15 No Hit GTTCCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGA 6 0.15 No Hit CCACATCCGACCGGGGCGCATCGCCGGCCCCCATCCACTTCCCTCCCGAC 5 0.125 No Hit GTCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCC 5 0.125 No Hit CCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAATTCCGAAGGTCTAAA 5 0.125 No Hit CCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTCCACC 5 0.125 No Hit CTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATT 5 0.125 No Hit GTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTT 5 0.125 No Hit CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA 5 0.125 No Hit GCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTA 5 0.125 No Hit CTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACAC 5 0.125 No Hit ATCCACTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGT 5 0.125 No Hit GGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCG 5 0.125 No Hit CTCATCTTGGGGTGGGCTTACTACTTAGATGCTTTCAGCAGTTATCCGCT 5 0.125 No Hit GTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 41 0.0 0.0 0.0 0.0 0.0 42 0.0 0.0 0.0 0.0 0.0 43 0.0 0.0 0.0 0.0 0.0 44 0.0 0.0 0.0 0.0 0.0 45 0.0 0.0 0.0 0.0 0.0 46 0.0 0.0 0.0 0.0 0.0 47 0.0 0.0 0.0 0.0 0.0 48 0.0 0.0 0.0 0.0 0.0 49 0.0 0.0 0.0 0.0 0.0 50 0.0 0.0 0.0 0.0 0.0 51 0.0 0.0 0.0 0.0 0.0 52 0.0 0.0 0.0 0.0 0.0 53 0.0 0.0 0.0 0.0 0.0 54 0.0 0.0 0.0 0.0 0.0 55 0.0 0.0 0.0 0.0 0.0 56 0.0 0.0 0.0 0.0 0.0 57 0.0 0.0 0.0 0.0 0.0 58 0.0 0.0 0.0 0.0 0.0 59 0.0 0.0 0.0 0.0 0.0 60 0.0 0.0 0.0 0.0 0.0 61 0.0 0.0 0.0 0.0 0.0 62 0.0 0.0 0.0 0.0 0.0 63 0.0 0.0 0.0 0.0 0.0 64 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR9668923 read2 length is 35-76 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR9668923_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 35-76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.159 32.0 32.0 32.0 32.0 32.0 2 30.95375 32.0 32.0 32.0 32.0 32.0 3 30.92025 32.0 32.0 32.0 32.0 32.0 4 31.00175 32.0 32.0 32.0 32.0 32.0 5 30.912 32.0 32.0 32.0 32.0 32.0 6 34.25475 36.0 36.0 36.0 32.0 36.0 7 34.50575 36.0 36.0 36.0 32.0 36.0 8 34.35775 36.0 36.0 36.0 32.0 36.0 9 34.46525 36.0 36.0 36.0 32.0 36.0 10-11 34.351875 36.0 36.0 36.0 32.0 36.0 12-13 34.39075 36.0 36.0 36.0 32.0 36.0 14-15 34.355125 36.0 36.0 36.0 32.0 36.0 16-17 34.299499999999995 36.0 36.0 36.0 32.0 36.0 18-19 34.224875 36.0 36.0 36.0 32.0 36.0 20-21 34.2235 36.0 36.0 36.0 32.0 36.0 22-23 34.186375 36.0 36.0 36.0 32.0 36.0 24-25 34.21787500000001 36.0 36.0 36.0 32.0 36.0 26-27 34.1235 36.0 36.0 36.0 32.0 36.0 28-29 34.191125 36.0 36.0 36.0 32.0 36.0 30-31 34.09375 36.0 36.0 36.0 32.0 36.0 32-33 34.164125 36.0 36.0 36.0 32.0 36.0 34-35 34.005875 36.0 36.0 36.0 32.0 36.0 36-37 34.16412118177266 36.0 36.0 36.0 32.0 36.0 38-39 34.01001753067869 36.0 36.0 36.0 32.0 36.0 40-41 33.93701477585775 36.0 36.0 36.0 29.5 36.0 42-43 33.840095166541445 36.0 36.0 36.0 29.5 36.0 44-45 33.74201517861668 36.0 36.0 36.0 27.0 36.0 46-47 33.84243486973948 36.0 36.0 36.0 29.5 36.0 48-49 34.014160401002506 36.0 36.0 36.0 32.0 36.0 50-51 33.92418546365915 36.0 36.0 36.0 32.0 36.0 52-53 33.825438596491225 36.0 36.0 36.0 32.0 36.0 54-55 33.80689223057644 36.0 36.0 36.0 29.5 36.0 56-57 33.73320802005013 36.0 36.0 36.0 29.5 36.0 58-59 33.63361744798195 36.0 36.0 36.0 29.5 36.0 60-61 33.59442677923607 36.0 36.0 36.0 27.0 36.0 62-63 33.65788813644344 36.0 36.0 36.0 27.0 36.0 64-65 33.582768999247556 36.0 36.0 36.0 27.0 36.0 66-67 33.51490716849837 36.0 36.0 36.0 27.0 36.0 68-69 33.689006024096386 36.0 36.0 36.0 27.0 36.0 70-71 33.618263864095255 36.0 36.0 36.0 27.0 36.0 72-73 33.55658618620264 36.0 36.0 36.0 27.0 36.0 74-75 33.64920708741643 36.0 36.0 36.0 27.0 36.0 76 32.25382605449795 36.0 32.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 0.0 15 4.0 16 3.0 17 8.0 18 12.0 19 10.0 20 8.0 21 7.0 22 18.0 23 17.0 24 25.0 25 37.0 26 38.0 27 57.0 28 67.0 29 84.0 30 124.0 31 167.0 32 196.0 33 320.0 34 689.0 35 2102.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.18364274962368 16.93426994480682 12.042147516307075 32.83993978926242 2 32.84031085485084 22.662321383805466 29.48107295061419 15.016294810729505 3 25.945404457801153 24.217380415727526 25.920360631104433 23.916854495366895 4 28.01702553830746 32.54882323485228 18.953430145217826 20.480721081622434 5 31.372058087130693 33.475212819228844 17.47621432148222 17.676514772158235 6 24.71206810215323 35.77866800200301 19.15373059589384 20.355533299949926 7 24.2864296444667 17.501251877816728 35.678517776665 22.533800701051575 8 26.61492238357536 22.25838758137206 24.3114672008012 26.81522283425138 9 27.290936404606907 22.108162243365047 26.765147721582373 23.83575363044567 10-11 28.55533299949925 28.392588883324986 19.55433149724587 23.497746619929895 12-13 28.45518277416124 22.8592889334001 23.29744616925388 25.38808212318478 14-15 27.929394091136707 25.375563345017525 24.43665498247371 22.25838758137206 16-17 28.782565130260522 26.365230460921847 21.993987975951903 22.85821643286573 18-19 28.467701552328496 25.751126690035054 23.172258387581373 22.608913370055085 20-21 29.159884812820835 24.752723175159634 23.81369725804432 22.27369475397521 22-23 27.919799498746865 26.453634085213036 22.218045112781954 23.408521303258144 24-25 28.42118442469012 25.892074621259546 23.28784274445975 22.398898209590584 26-27 28.15473209814722 26.189283925888834 22.070605908863293 23.585378067100653 28-29 28.660904421896532 26.706751847676312 22.610547413253162 22.021796317173994 30-31 29.21418724150896 24.639679157789196 22.133099385887956 24.013034214813885 32-33 26.759328825444527 27.08489857250188 22.96518908089156 23.190583521162033 34-35 27.758987849179505 26.706751847676312 22.785920080170364 22.74834022297382 36-37 27.752696262854275 25.984449460747427 22.648607975921745 23.614246300476548 38-39 27.28413654618474 26.48092369477912 22.79116465863454 23.443775100401606 40-41 29.058217025022003 26.078209480699105 22.60782094806991 22.255752546208978 42-43 28.495909376966644 25.726872246696036 22.743864065449966 23.03335431088735 44-45 27.710539586485122 26.638930912758447 23.00806858295512 22.64246091780131 46-47 27.814152606396377 25.736590279526567 22.48803827751196 23.961218836565095 48-49 28.293601003764113 26.097867001254706 22.672521957340024 22.936010037641154 50-51 28.59473023839398 27.27728983688833 22.33375156838143 21.794228356336262 52-53 29.613356766256587 25.897564649761485 22.834546824002008 21.654531759979914 54-55 29.17661847894406 25.45568824638592 23.016970458830922 22.350722815839095 56-57 28.312570781426956 26.953567383918458 22.25997231659746 22.473889518057128 58-59 28.492323181474955 25.799144223508684 23.68487289202114 22.023659702995218 60-61 28.908406524466752 24.642409033877037 23.28732747804266 23.161856963613552 62-63 28.718463662608258 24.58892933350069 23.873478097150745 22.819128906740303 64-65 28.72059378538181 25.978110454145174 23.449490501949928 21.851805258523086 66-67 29.126824358329138 25.188726723704075 22.697533970810266 22.986914947156517 68-69 28.314691466633153 25.248209124041725 22.885509614176197 23.551589795148924 70-71 28.047708725674823 24.243565599497803 23.364720652856246 24.344005021971125 72-73 28.539042821158688 24.861460957178842 23.02267002518892 23.57682619647355 74-75 29.690005344735436 20.978086584714056 24.251737039016568 25.080171031533936 76 32.88540500186637 0.0 35.27435610302352 31.84023889511012 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6.0 1 3.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 1.0 20 1.5 21 0.5 22 1.0 23 1.0 24 1.5 25 3.5 26 3.5 27 3.5 28 7.0 29 11.5 30 11.0 31 11.5 32 14.0 33 17.0 34 25.0 35 36.0 36 47.0 37 55.0 38 70.5 39 92.0 40 112.0 41 148.5 42 168.0 43 171.5 44 187.5 45 200.5 46 208.0 47 225.5 48 245.0 49 233.0 50 212.5 51 194.0 52 197.5 53 192.0 54 168.5 55 198.5 56 223.0 57 203.5 58 176.0 59 171.0 60 204.5 61 184.0 62 137.5 63 100.0 64 52.5 65 39.5 66 36.0 67 33.0 68 30.5 69 26.0 70 16.5 71 9.5 72 16.0 73 19.0 74 10.5 75 5.5 76 6.5 77 6.5 78 3.5 79 3.0 80 4.0 81 2.5 82 1.5 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 1.0 91 2.0 92 2.0 93 1.5 94 0.5 95 0.5 96 1.0 97 0.5 98 0.5 99 10.5 100 20.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.27499999999999997 3 0.17500000000000002 4 0.15 5 0.15 6 0.15 7 0.15 8 0.15 9 0.15 10-11 0.15 12-13 0.15 14-15 0.15 16-17 0.2 18-19 0.15 20-21 0.1625 22-23 0.25 24-25 0.1625 26-27 0.15 28-29 0.21250000000000002 30-31 0.2625 32-33 0.17500000000000002 34-35 0.21250000000000002 36-37 0.17526289434151227 38-39 0.2253944402704733 40-41 0.4132231404958678 42-43 0.5133984472827448 44-45 0.6637445209768316 46-47 0.5260521042084169 48-49 0.12531328320802004 50-51 0.12531328320802004 52-53 0.17543859649122806 54-55 0.3132832080200501 56-57 0.41353383458646614 58-59 0.4011030333416896 60-61 0.07522567703109327 62-63 0.08778530223225482 64-65 0.3135189365437672 66-67 0.3010536879076769 68-69 0.13805220883534136 70-71 0.012553351744915892 72-73 0.050352467270896276 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 35 6.0 36 0.0 37 1.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 1.0 45 0.0 46 0.0 47 2.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 1.0 58 0.0 59 0.0 60 2.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 2.0 67 1.0 68 0.0 69 0.0 70 2.0 71 4.0 72 12.0 73 62.0 74 324.0 75 901.0 76 2679.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.8 #Duplication Level Percentage of deduplicated Percentage of total 1 89.16062801932367 73.825 2 7.065217391304348 11.700000000000001 3 1.9021739130434785 4.725 4 0.9057971014492754 3.0 5 0.36231884057971014 1.5 6 0.2113526570048309 1.05 7 0.15096618357487923 0.8750000000000001 8 0.06038647342995169 0.4 9 0.0 0.0 >10 0.18115942028985507 2.9250000000000003 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCAAGGAAGCTGACTGGCGGGATCCCCTCG 30 0.75 No Hit CTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCAAGGAA 23 0.575 No Hit GGAGTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCA 22 0.5499999999999999 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 19 0.475 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 12 0.3 No Hit GTTAGTTTTACCCTACTGATGACAGTGTCGCAATAGTAATCCAACCTAGTACGAGAGGAACCGTTGATTCGCACA 11 0.27499999999999997 No Hit CGGGACTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAAC 8 0.2 No Hit GTGAACTATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCC 8 0.2 No Hit CGAGAACAGAAATCTCGTGTGGAACAAAAGGGTAAAAGCTCGTTTGATTC 7 0.17500000000000002 No Hit GTCAGGCGGGACTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCCCTAGT 7 0.17500000000000002 No Hit CGCCGACCGACCTTGATCTTCTGAGAAGGGTTCGAGTGAGAGCATGCCTG 7 0.17500000000000002 No Hit GGGAAATGCCCAGCTTGAGAATCTGGCGCCTGCGGCGTCCGAATTGTAGT 7 0.17500000000000002 No Hit CGCAAGGAAGCTGACTGGCGGGATCCCCTCGAGGGTTGCACCGCCGACCGACCTTGATCTTCTGAGAAGGGTTCG 7 0.17500000000000002 No Hit CTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTT 6 0.15 No Hit GGTGAACTATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGC 6 0.15 No Hit CAACGCCCTCGACCTATTCTCAAACTTTAAATAGGTAGGACGGCGCGGCT 6 0.15 No Hit CAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTC 6 0.15 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6 0.15 No Hit CGGCGCGGCTGCTTCGTTGAGCCGCGCCACGGAATCGAGAGCTCCAAGTG 6 0.15 No Hit GGCTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCGACGAAATGCTTCGGGGAGTTGAA 6 0.15 No Hit CTTGATCTTCTGAGAAGGGTTCGAGTGAGAGCATGCCTGTCGGGACCCGA 5 0.125 No Hit CGAATACGAACCGTGAAAGCGTGGCCTATCGATCCTTTAGACCTTCGGAA 5 0.125 No Hit CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAG 5 0.125 No Hit GTTGTGGTTAGGGGTGAAATGCCACTCGAACCCAGAGCTAGCTGGTTCTC 5 0.125 No Hit GTAAGCTCCCAAGCAGTGGGAGGAGCCCGGGGCTCTGACCGCGTGCCTGT 5 0.125 No Hit AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG 5 0.125 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAA 5 0.125 No Hit GACTAATCGAACCGTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA 5 0.125 No Hit AGGAGTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGC 5 0.125 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 5 0.125 No Hit CCGAAGTTTCCCTCAGGATAGCTGGAGCTCGGTGCGAGTTCTATCGGGTA 5 0.125 No Hit AAGGAGTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 41 0.0 0.0 0.0 0.0 0.0 42 0.0 0.0 0.0 0.0 0.0 43 0.0 0.0 0.0 0.0 0.0 44 0.0 0.0 0.0 0.0 0.0 45 0.0 0.0 0.0 0.0 0.0 46 0.0 0.0 0.0 0.0 0.0 47 0.0 0.0 0.0 0.0 0.0 48 0.0 0.0 0.0 0.0 0.0 49 0.0 0.0 0.0 0.0 0.0 50 0.0 0.0 0.0 0.0 0.0 51 0.0 0.0 0.0 0.0 0.0 52 0.0 0.0 0.0 0.0 0.0 53 0.0 0.0 0.0 0.0 0.0 54 0.0 0.0 0.0 0.0 0.0 55 0.0 0.0 0.0 0.0 0.0 56 0.0 0.0 0.0 0.0 0.0 57 0.0 0.0 0.0 0.0 0.0 58 0.0 0.0 0.0 0.0 0.0 59 0.0 0.0 0.0 0.0 0.0 60 0.0 0.0 0.0 0.0 0.0 61 0.0 0.0 0.0 0.0 0.0 62 0.0 0.0 0.0 0.0 0.0 63 0.0 0.0 0.0 0.0 0.0 64 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGAGACA 20 0.006649303 52.115627 23 CGGGAAG 20 0.006649303 52.115627 57 >>END_MODULE Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra Read 1631067 spots for SRR9668923.sra Written 1631067 spots for SRR9668923.sra Read 1631055 spots for SRR9668923.sra Written 1631055 spots for SRR9668923.sra SRR ids: ['SRR9668923.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_8ha24ysx SRR9668923.sra spots: 32621112 blocks: [[1, 1631055], [1631056, 3262110], [3262111, 4893165], [4893166, 6524220], [6524221, 8155275], [8155276, 9786330], [9786331, 11417385], [11417386, 13048440], [13048441, 14679495], [14679496, 16310550], [16310551, 17941605], [17941606, 19572660], [19572661, 21203715], [21203716, 22834770], [22834771, 24465825], [24465826, 26096880], [26096881, 27727935], [27727936, 29358990], [29358991, 30990045], [30990046, 32621112]] SRR9668923 file size 6194650 SRR9668923 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR9668923 SRR9668923_1.fastq SRR9668923_2.fastq Input file: SRR9668923_1.fastq Paired file: SRR9668923_2.fastq trimmed: SRR9668923-trimmed-pair1.fastq, SRR9668923-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 16:39:16 2025 >> started Wed Feb 12 16:39:42 2025 >> done (25.354s) 32621112 read pairs processed; of these: 17 ( 0.00%) short read pairs filtered out after trimming by size control 5813 ( 0.02%) empty read pairs filtered out after trimming by size control 32615282 (99.98%) read pairs available; of these: 4231 ( 0.01%) trimmed read pairs available after processing 32611051 (99.99%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 21 1 0.00% 22 0 0.00% 23 2 0.00% 24 4 0.00% 25 3 0.00% 26 9 0.00% 27 5 0.00% 28 13 0.00% 29 6 0.00% 30 6 0.00% 31 13 0.00% 32 7 0.00% 33 8 0.00% 34 10 0.00% 35 115 0.00% 36 127 0.00% 37 148 0.00% 38 190 0.00% 39 188 0.00% 40 197 0.00% 41 267 0.00% 42 268 0.00% 43 311 0.00% 44 337 0.00% 45 340 0.00% 46 349 0.00% 47 450 0.00% 48 492 0.00% 49 623 0.00% 50 734 0.00% 51 915 0.00% 52 995 0.00% 53 981 0.00% 54 1153 0.00% 55 1496 0.00% 56 1570 0.00% 57 1461 0.00% 58 1736 0.01% 59 1848 0.01% 60 2086 0.01% 61 2118 0.01% 62 2380 0.01% 63 2656 0.01% 64 2738 0.01% 65 2858 0.01% 66 3139 0.01% 67 3459 0.01% 68 3539 0.01% 69 3937 0.01% 70 5438 0.02% 71 10608 0.03% 72 20735 0.06% 73 204777 0.63% 74 2288535 7.02% 75 14745925 45.21% 76 15292976 46.89% 32615282 reads passed initial QC criterion=sequence-density sequence-density=0.76 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=24 prefix-density=0.76 prefix-fanout=2.0 sequence=ATTACCGCGGCTGCTGGCAC criterion=fanout-score sequence-density=0.04 sequence-density-rank=30 fanout-score=9.96 fanout-score-rank=1 prefix-density=0.07 prefix-fanout=5.8 sequence=GCTCCAAGCATGGCCCACCTGGAATGGATGACTTCAAGCTCACGGTTCTTGGCAAAGGTCTCTGGGTCAGCAGAGAGGCCAGCAGTGTCCCAGCCGTAGTCACCAGGGAACTCACCAGTCAAGTAGGATGGGGGCTCACCAGAGAACGGGCCCAAGTATTTAACACGGTCTGGTCCGTACCATGGGCTCCCGGAGGGAACAGGCTTGGTGGTTTTCCTCATGGAGACACGGCCATTGCCCATGATCTCAGAGGAGGAGGGGTTGAGCTTCACC criterion=sequence-density sequence-density=0.54 sequence-density-rank=1 fanout-score=2.23 fanout-score-rank=21 prefix-density=0.57 prefix-fanout=2.1 sequence=GTGCCAGCAGCCGCGGTAAT criterion=fanout-score sequence-density=0.03 sequence-density-rank=30 fanout-score=22.18 fanout-score-rank=1 prefix-density=0.32 prefix-fanout=1.9 sequence=GGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAAGAGACGGGGGAAGCCCGTCCGACAGCGCGTTCGCGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTCCTGAACCGGGACGTGGCGGCTGACGGCAACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCCGCCCACCCTGGAAACGACTTAGTCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCGCACGTCGCGTGGTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCTCCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGG SRR9668923 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 16:40:12 Started mapping on | Feb 12 16:40:12 Finished on | Feb 12 16:45:57 Mapping speed, Million of reads per hour | 340.33 Number of input reads | 32615282 Average input read length | 151 UNIQUE READS: Uniquely mapped reads number | 17704107 Uniquely mapped reads % | 54.28% Average mapped length | 150.09 Number of splices: Total | 5594029 Number of splices: Annotated (sjdb) | 5527207 Number of splices: GT/AG | 5487245 Number of splices: GC/AG | 89730 Number of splices: AT/AC | 4363 Number of splices: Non-canonical | 12691 Mismatch rate per base, % | 0.78% Deletion rate per base | 0.01% Deletion average length | 2.20 Insertion rate per base | 0.04% Insertion average length | 2.54 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1565624 % of reads mapped to multiple loci | 4.80% Number of reads mapped to too many loci | 11973541 % of reads mapped to too many loci | 36.71% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.55% % of reads unmapped: other | 1.66% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 13345581 13345581 13345581 N_multimapping 1565624 1565624 1565624 N_noFeature 4016173 17131390 4113441 N_ambiguous 564555 3204 86171 UnstrandedReadsAssigned:13123379 PositiveStrandReadsAssigned:569513 NegativeStrandReadsAssigned:13504495 Dataset is classified negative stranded MeadianReadLen=76 20thPercentileLength=75 echo kmer=71 SRR9668923 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR9668923-trimmed-pair1.fastq SRR9668923-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 32,615,282 reads, 24,211,881 reads pseudoaligned [quant] estimated average fragment length: 213.052 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,155 rounds 52401 SRR9668923.ke.tsv 34699 SRR9668923.se.tsv 87100 total ==> SRR9668923.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1805.95 213 2.60482 Potri.005G024800.1.v4.1 1035 822.948 51 1.36868 Potri.004G059700.1.v4.1 961 748.948 15 0.442326 Potri.007G009000.2.v4.1 1416 1203.95 0 0 Potri.003G141000.2.v4.1 2943 2730.95 196 1.58506 Potri.016G087400.1.v4.1 270 80.583 857 234.877 Potri.015G069301.1.v4.1 564 352.054 0 0 Potri.010G195200.1.v4.1 1773 1560.95 4 0.0565946 Potri.012G127500.1.v4.1 977 764.948 2002 57.801 ==> SRR9668923.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 10 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 214 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 1 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 50 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 7 SRR9668923 completed mapping pipeline successfully