Starting /dee2/code/volunteer_pipeline.sh SRR30508881
    current disk space = 1258791504
    free memory = 62705008 
SRR30508881 SRAfilesize
75690818472049ceae847ad1aa7d2cf4  SRR30508881.sra
SRR30508881.sra file validated
SRR30508881 is paired end
SRR30508881 is conventional basespace
SRR30508881 read1 length is 75 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR30508881_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	75
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.512	32.0	32.0	32.0	32.0	32.0
2	31.807	32.0	32.0	32.0	32.0	32.0
3	35.79425	37.0	37.0	37.0	32.0	37.0
4	36.294	37.0	37.0	37.0	37.0	37.0
5	36.6645	37.0	37.0	37.0	37.0	37.0
6	41.025	42.0	42.0	42.0	37.0	42.0
7	40.966	42.0	42.0	42.0	37.0	42.0
8	41.3705	42.0	42.0	42.0	42.0	42.0
9	41.29025	42.0	42.0	42.0	42.0	42.0
10	41.027	42.0	42.0	42.0	37.0	42.0
11	41.0435	42.0	42.0	42.0	37.0	42.0
12	41.15325	42.0	42.0	42.0	37.0	42.0
13	40.92875	42.0	42.0	42.0	37.0	42.0
14	41.136	42.0	42.0	42.0	37.0	42.0
15	41.4425	42.0	42.0	42.0	42.0	42.0
16	41.3265	42.0	42.0	42.0	42.0	42.0
17	41.3515	42.0	42.0	42.0	42.0	42.0
18	41.369	42.0	42.0	42.0	42.0	42.0
19	41.39175	42.0	42.0	42.0	42.0	42.0
20	41.358	42.0	42.0	42.0	42.0	42.0
21	41.28225	42.0	42.0	42.0	42.0	42.0
22	40.9875	42.0	42.0	42.0	37.0	42.0
23	41.01175	42.0	42.0	42.0	42.0	42.0
24	41.137	42.0	42.0	42.0	42.0	42.0
25	41.18775	42.0	42.0	42.0	42.0	42.0
26	41.18225	42.0	42.0	42.0	42.0	42.0
27	41.223	42.0	42.0	42.0	42.0	42.0
28	41.1055	42.0	42.0	42.0	42.0	42.0
29	41.279	42.0	42.0	42.0	42.0	42.0
30	41.11175	42.0	42.0	42.0	42.0	42.0
31	40.87425	42.0	42.0	42.0	37.0	42.0
32	41.02425	42.0	42.0	42.0	37.0	42.0
33	40.92825	42.0	42.0	42.0	37.0	42.0
34	41.0685	42.0	42.0	42.0	42.0	42.0
35	41.1925	42.0	42.0	42.0	42.0	42.0
36	41.148	42.0	42.0	42.0	42.0	42.0
37	41.1065	42.0	42.0	42.0	42.0	42.0
38	40.881	42.0	42.0	42.0	37.0	42.0
39	40.986	42.0	42.0	42.0	37.0	42.0
40	41.1795	42.0	42.0	42.0	42.0	42.0
41	41.124	42.0	42.0	42.0	42.0	42.0
42	40.9165	42.0	42.0	42.0	37.0	42.0
43	40.8535	42.0	42.0	42.0	37.0	42.0
44	40.86075	42.0	42.0	42.0	37.0	42.0
45	40.75275	42.0	42.0	42.0	37.0	42.0
46	40.6235	42.0	42.0	42.0	37.0	42.0
47	40.85	42.0	42.0	42.0	37.0	42.0
48	40.92925	42.0	42.0	42.0	37.0	42.0
49	40.8815	42.0	42.0	42.0	37.0	42.0
50	40.6475	42.0	42.0	42.0	37.0	42.0
51	40.50675	42.0	42.0	42.0	37.0	42.0
52	40.5605	42.0	42.0	42.0	37.0	42.0
53	40.628	42.0	42.0	42.0	37.0	42.0
54	40.6505	42.0	42.0	42.0	37.0	42.0
55	40.31525	42.0	42.0	42.0	37.0	42.0
56	40.4015	42.0	42.0	42.0	37.0	42.0
57	40.546	42.0	42.0	42.0	37.0	42.0
58	40.716	42.0	42.0	42.0	37.0	42.0
59	40.14	42.0	42.0	42.0	37.0	42.0
60	39.9685	42.0	42.0	42.0	37.0	42.0
61	39.94375	42.0	42.0	42.0	37.0	42.0
62	40.09375	42.0	42.0	42.0	37.0	42.0
63	40.59575	42.0	42.0	42.0	37.0	42.0
64	40.1195	42.0	42.0	42.0	37.0	42.0
65	39.84975	42.0	42.0	42.0	37.0	42.0
66	40.04075	42.0	42.0	42.0	37.0	42.0
67	40.0485	42.0	42.0	42.0	37.0	42.0
68	39.96525	42.0	42.0	42.0	37.0	42.0
69	40.3215	42.0	42.0	42.0	37.0	42.0
70	39.9925	42.0	42.0	42.0	37.0	42.0
71	39.897	42.0	42.0	42.0	37.0	42.0
72	40.2555	42.0	42.0	42.0	37.0	42.0
73	40.24925	42.0	42.0	42.0	37.0	42.0
74	40.05875	42.0	42.0	42.0	37.0	42.0
75	40.0285	42.0	42.0	42.0	37.0	42.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
1101	52	0.0
1101	53	0.0
1101	54	0.0
1101	55	0.0
1101	56	0.0
1101	57	0.0
1101	58	0.0
1101	59	0.0
1101	60	0.0
1101	61	0.0
1101	62	0.0
1101	63	0.0
1101	64	0.0
1101	65	0.0
1101	66	0.0
1101	67	0.0
1101	68	0.0
1101	69	0.0
1101	70	0.0
1101	71	0.0
1101	72	0.0
1101	73	0.0
1101	74	0.0
1101	75	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	1.0
25	3.0
26	1.0
27	4.0
28	8.0
29	13.0
30	15.0
31	27.0
32	27.0
33	29.0
34	51.0
35	52.0
36	76.0
37	125.0
38	132.0
39	230.0
40	596.0
41	2609.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.625	17.65	11.575000000000001	29.15
2	23.674999999999997	25.25	27.950000000000003	23.125
3	20.9	34.75	20.125	24.224999999999998
4	26.3	36.525	18.6	18.575
5	20.65	41.3	20.724999999999998	17.325
6	18.25	41.099999999999994	20.349999999999998	20.3
7	14.95	25.874999999999996	40.6	18.575
8	19.25	28.799999999999997	23.474999999999998	28.475
9	19.1	24.099999999999998	29.575000000000003	27.224999999999998
10	18.4	41.65	21.224999999999998	18.725
11	25.074999999999996	27.575	18.275	29.075
12	23.05	23.625	25.55	27.775
13	19.975	31.6	24.8	23.625
14	20.825	31.2	26.700000000000003	21.275
15	21.475	29.625	25.874999999999996	23.025000000000002
16	21.575	30.15	26.325	21.95
17	20.424999999999997	33.725	26.0	19.85
18	20.849999999999998	30.675	24.525	23.95
19	18.0	34.875	25.074999999999996	22.05
20	18.775	31.4	27.500000000000004	22.325
21	19.1	29.7	26.974999999999998	24.224999999999998
22	18.9	34.625	25.35	21.125
23	21.45545796737767	36.060225846925974	23.914680050188206	18.569636135508155
24	18.125	34.150000000000006	25.35	22.375
25	19.7	30.65	25.575	24.075
26	20.5	34.1	24.875	20.525
27	18.175	33.475	26.825	21.525
28	22.7	31.724999999999998	24.75	20.825
29	19.825	31.3	25.474999999999998	23.400000000000002
30	20.3	34.075	24.349999999999998	21.275
31	19.55	34.55	23.400000000000002	22.5
32	18.55	32.45	26.700000000000003	22.3
33	21.55	33.0	23.625	21.825
34	20.525	31.624999999999996	25.3	22.55
35	20.275000000000002	33.6	23.799999999999997	22.325
36	23.35	31.65	23.400000000000002	21.6
37	21.25	31.324999999999996	25.15	22.275
38	21.175	30.075000000000003	28.925	19.825
39	20.549999999999997	28.7	30.0	20.75
40	19.45	30.9	27.224999999999998	22.425
41	19.225	32.675	25.85	22.25
42	20.65	30.325000000000003	28.050000000000004	20.974999999999998
43	21.425	33.074999999999996	25.275	20.225
44	19.675	31.275	27.625	21.425
45	23.474999999999998	31.25	24.4	20.875
46	18.275	34.275	26.174999999999997	21.275
47	19.725	34.449999999999996	25.5	20.325
48	21.025	31.275	26.825	20.875
49	22.425	30.375000000000004	26.325	20.875
50	21.099999999999998	31.324999999999996	26.700000000000003	20.875
51	21.125	30.65	27.6	20.625
52	20.625	29.7	26.974999999999998	22.7
53	20.25	28.975	30.075000000000003	20.7
54	21.725	29.599999999999998	26.474999999999998	22.2
55	18.05	31.1	29.975	20.875
56	19.275000000000002	30.475	29.925	20.325
57	18.4	30.025000000000002	27.275	24.3
58	22.175	33.525	22.175	22.125
59	21.625	32.875	24.9	20.599999999999998
60	18.875	32.2	28.925	20.0
61	20.8	31.825	26.3	21.075
62	19.025	30.2	26.575	24.2
63	21.675	30.099999999999998	24.7	23.525
64	20.349999999999998	31.3	25.15	23.200000000000003
65	19.45	33.2	26.1	21.25
66	19.25	35.8	25.75	19.2
67	19.525000000000002	31.724999999999998	27.725	21.025
68	20.674999999999997	29.95	26.724999999999998	22.650000000000002
69	19.7	28.725	26.525	25.05
70	20.974999999999998	31.924999999999997	26.150000000000002	20.95
71	20.325	32.375	26.424999999999997	20.875
72	21.15	29.65	26.674999999999997	22.525000000000002
73	19.5	31.974999999999998	23.9	24.625
74	19.85	33.75	24.25	22.15
75	20.775	31.724999999999998	25.85	21.65
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	1.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	0.5
18	0.0
19	1.5
20	3.0
21	5.0
22	6.0
23	7.5
24	10.0
25	10.5
26	16.0
27	22.0
28	23.0
29	26.0
30	32.5
31	42.0
32	48.0
33	64.0
34	99.0
35	128.0
36	138.0
37	195.0
38	321.0
39	404.5
40	419.0
41	418.0
42	345.5
43	318.0
44	362.0
45	295.0
46	267.0
47	231.5
48	157.0
49	138.5
50	126.5
51	112.5
52	92.0
53	88.5
54	66.0
55	43.0
56	39.0
57	45.0
58	42.0
59	28.0
60	23.0
61	22.5
62	17.0
63	7.0
64	2.0
65	1.0
66	0.0
67	0.0
68	0.0
69	0.5
70	0.5
71	0.5
72	1.0
73	0.5
74	0.5
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.375
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.8
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.98203592814372	56.10000000000001
2	8.233532934131736	11.0
3	2.9940119760479043	6.0
4	1.0853293413173652	2.9000000000000004
5	1.0479041916167664	3.5000000000000004
6	0.561377245508982	2.25
7	0.2619760479041916	1.225
8	0.4491017964071856	2.4
9	0.18712574850299402	1.125
>10	1.1601796407185627	12.025
>50	0.037425149700598806	1.4749999999999999
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCCGAATACACCAGCTACACCTAACATGTGAAATGGGTGCATAAGAATGTTGTGCTCAGCCTGGAATACAATCAT	59	1.4749999999999999	No Hit
TAAGAATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACCATCAG	27	0.675	No Hit
CTCAACAGCAGCTAGGTCTAGAGGGAAGTTGTGAGCATTACGTTCATGCATAACTTCCATACCAAGGTTAGCACG	26	0.65	No Hit
GGCCAAGCCGCTAAGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAAAATCAATCGGCCAAAA	26	0.65	No Hit
GCGGTCAATAAGGTAGGGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAGTGCTAGTTATCCAG	25	0.625	No Hit
GTTGAAACTAGCATATTGGAAAATCAATCGGCCAAAATAACCGTGAGCAGCTACAATGTTGTAAGTTTCTTCTTC	23	0.575	No Hit
GTTTTTTACTAAACTAGATCTAGACTAACACTAACGAATTATCCATTTGTAGATGGAGCCTCAACAGCAGCTAGG	20	0.5	No Hit
GGTCAATAAGGTAGGGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAGTGCTAGTTATCCAGTT	19	0.475	No Hit
GTGCATAAGAATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACC	18	0.44999999999999996	No Hit
CAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAACACCAAACCATCCAA	18	0.44999999999999996	No Hit
CCGGCCAAGCCGCTAAGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAAAATCAATCGGCCAA	18	0.44999999999999996	No Hit
GGAGCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAACACCAAACCAT	16	0.4	No Hit
CAATAAGGTAGGGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAGTGCTAGTTATCCAGTTACA	16	0.4	No Hit
GGAAAATCAATCGGCCAAAATAACCGTGAGCAGCTACAATGTTGTAAGTTTCTTCTTCTTGCCCGAATCTGTAAC	16	0.4	No Hit
GTAGGGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAGTGCTAGTTATCCAGTTACAGAAGCGA	14	0.35000000000000003	No Hit
GGAGGAGCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAACACCAAAC	14	0.35000000000000003	No Hit
GCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAACACCAAACCATCCA	13	0.325	No Hit
CGGAAACTAAGTTCCCACTCACGACCCATATAACAAGCTACACCAAGTAAAAAGTGTAGAACAATTAGTTCATAA	13	0.325	No Hit
GAAAAACTTCCCTGACCAATTGGATAGATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCAACA	13	0.325	No Hit
GCAGCTACAATGTTGTAAGTTTCTTCTTCTTGCCCGAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTT	13	0.325	No Hit
GGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAGTGCTAGTTATCCAGTTACAGAAGCGACCCC	12	0.3	No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCCTGACCA	12	0.3	No Hit
GTCAATAAGGTAGGGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAGTGCTAGTTATCCAGTTA	12	0.3	No Hit
CTACAATGTTGTAAGTTTCTTCTTCTTGCCCGAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTTTCCC	12	0.3	No Hit
CAAGGAACCATGCATAGCACTAAAAAGGGAGCCGCCGAATACACCAGCTACACCTAACATGTGAAATGGGTGCAT	11	0.27499999999999997	No Hit
CGAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTTTCCCTGATCAAACTAGAAGTTACCAAGGAACCAT	11	0.27499999999999997	No Hit
GCTGAATATGCAACAGCAATCCAAGGACGCATACCCAGACGGAAACTAAGTTCCCACTCACGACCCATATAACAA	11	0.27499999999999997	No Hit
AGAATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACCATCAGAA	11	0.27499999999999997	No Hit
TGGAGGAGCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAACACCAAA	11	0.27499999999999997	No Hit
GCCAAAATAACCGTGAGCAGCTACAATGTTGTAAGTTTCTTCTTCTTGCCCGAATCTGTAACCTTCATTAGCAGA	10	0.25	No Hit
CGGCCAAGCCGCTAAGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAAAATCAATCGGCCAAA	10	0.25	No Hit
CAAGCCGCTAAGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAAAATCAATCGGCCAAAATAA	10	0.25	No Hit
GTCCAATCGGAGCGGCTTATTGGGTCATAGTGGGCTCGGAAGGCAATGGCCCGTAATCCTTCCACCGTCAAGAGC	9	0.22499999999999998	No Hit
CTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCCTGACCAAT	9	0.22499999999999998	No Hit
AAGAATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACCATCAGA	9	0.22499999999999998	No Hit
AGCAGCTACAATGTTGTAAGTTTCTTCTTCTTGCCCGAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGT	9	0.22499999999999998	No Hit
CACGAATACCATCAATATCTACTGGAGGAGCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGG	9	0.22499999999999998	No Hit
CTCGTTTTTTACTAAACTAGATCTAGACTAACACTAACGAATTATCCATTTGTAGATGGAGCCTCAACAGCAGCT	8	0.2	No Hit
GGAAACTAAGTTCCCACTCACGACCCATATAACAAGCTACACCAAGTAAAAAGTGTAGAACAATTAGTTCATAAG	8	0.2	No Hit
CAAATACCTACTACCGGCCAAGCCGCTAAGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAAA	8	0.2	No Hit
CGGTTTTCAGTGCTAGTTATCCAGTTACAGAAGCGACCCCATAGGCTTTCGCTTTCGCGTCTCTCTAAAATTGCA	8	0.2	No Hit
ACGGAAACTAAGTTCCCACTCACGACCCATATAACAAGCTACACCAAGTAAAAAGTGTAGAACAATTAGTTCATA	8	0.2	No Hit
CAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGACGCATACCCAGACGGAAACTAAGTT	8	0.2	No Hit
CAGAAGCGACCCCATAGGCTTTCGCTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAAATCCTTGGTTTATTTA	8	0.2	No Hit
GCTAGGTCTAGAGGGAAGTTGTGAGCATTACGTTCATGCATAACTTCCATACCAAGGTTAGCACGGTTAATAATA	8	0.2	No Hit
AGCAGTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGACGCATACCCAGACGGAAACTAAGTTC	8	0.2	No Hit
GTTGTATAGCCATTCATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTAGG	8	0.2	No Hit
AAAAACTTCCCTGACCAATTGGATAGATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCAACAG	8	0.2	No Hit
GCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAACACCAAACCATCCAATG	8	0.2	No Hit
CTGGAGGAGCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAACACCAA	7	0.17500000000000002	No Hit
GCCAAGCCGCTAAGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAAAATCAATCGGCCAAAAT	7	0.17500000000000002	No Hit
GCCATTCATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTAGGAATAATGG	7	0.17500000000000002	No Hit
GCTCAGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCCT	7	0.17500000000000002	No Hit
GGAGCCTCAACAGCAGCTAGGTCTAGAGGGAAGTTGTGAGCATTACGTTCATGCATAACTTCCATACCAAGGTTA	7	0.17500000000000002	No Hit
GGTTTTCAGTGCTAGTTATCCAGTTACAGAAGCGACCCCATAGGCTTTCGCTTTCGCGTCTCTCTAAAATTGCAG	7	0.17500000000000002	No Hit
GCTTATATTGCTCGTTTTTTACTAAACTAGATCTAGACTAACACTAACGAATTATCCATTTGTAGATGGAGCCTC	7	0.17500000000000002	No Hit
CTAGAGGCATACCATCAGAAAAACTTCCCTGACCAATTGGATAGATCAAGAAAACAGCAGTCGCAGCTGCAACAG	6	0.15	No Hit
GCCGCTAAGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAAAATCAATCGGCCAAAATAACCG	6	0.15	No Hit
ATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTAGGAATAATGGCACCGGA	6	0.15	No Hit
GTGCTAGTTATCCAGTTACAGAAGCGACCCCATAGGCTTTCGCTTTCGCGTCTCTCTAAAATTGCAGTCATGGTA	6	0.15	No Hit
CTCAGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCCTG	6	0.15	No Hit
ATAATATCAGCCCAAGTATTAATAACACGTCCTTGACTATCAACTACTGATTGGTTGAAATTGAAACCATTTAGG	6	0.15	No Hit
TAGCCATTCATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTAGGAATAAT	6	0.15	No Hit
CCCGAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTTTCCCTGATCAAACTAGAAGTTACCAAGGAACC	6	0.15	No Hit
GCTAGTTATCCAGTTACAGAAGCGACCCCATAGGCTTTCGCTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAA	6	0.15	No Hit
GTTACAGAAGCGACCCCATAGGCTTTCGCTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAAATCCTTGGTTTA	6	0.15	No Hit
CGAATACCATCAATATCTACTGGAGGAGCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTA	6	0.15	No Hit
CCTCAACAGCAGCTAGGTCTAGAGGGAAGTTGTGAGCATTACGTTCATGCATAACTTCCATACCAAGGTTAGCAC	6	0.15	No Hit
GCATAAGAATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACCAT	6	0.15	No Hit
AGAAGCGACCCCATAGGCTTTCGCTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAAATCCTTGGTTTATTTAA	6	0.15	No Hit
ACAGAAGCGACCCCATAGGCTTTCGCTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAAATCCTTGGTTTATTT	6	0.15	No Hit
GCCTCAACAGCAGCTAGGTCTAGAGGGAAGTTGTGAGCATTACGTTCATGCATAACTTCCATACCAAGGTTAGCA	5	0.125	No Hit
CCGTTGTATAGCCATTCATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTA	5	0.125	No Hit
CTACTACCGGCCAAGCCGCTAAGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAAAATCAATC	5	0.125	No Hit
AAACAGGTTCACGAATACCATCAATATCTACTGGAGGAGCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGG	5	0.125	No Hit
GGGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAGTGCTAGTTATCCAGTTACAGAAGCGACCC	5	0.125	No Hit
GGCATACCATCAGAAAAACTTCCCTGACCAATTGGATAGATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCT	5	0.125	No Hit
GTAAAAATGCAATCCAATAGCTGCAGAAGTAGGAATAATGGCACCGGAAATAATATTGTTTCCGTAAAGAAGAGA	5	0.125	No Hit
ACCAGCTACACCTAACATGTGAAATGGGTGCATAAGAATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTGAA	5	0.125	No Hit
AAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAAAATCAATCGGCCAAAATAACCGTGAGCAGCT	5	0.125	No Hit
CAGCTACAATGTTGTAAGTTTCTTCTTCTTGCCCGAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTTT	5	0.125	No Hit
TCAATATCTACTGGAGGAGCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATC	5	0.125	No Hit
GTAACCTTCATTAGCAGATTCATTTTCTGTGGTTTCCCTGATCAAACTAGAAGTTACCAAGGAACCATGCATAGC	5	0.125	No Hit
GTATAGCCATTCATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTAGGAAT	5	0.125	No Hit
CATTCATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTAGGAATAATGGCA	5	0.125	No Hit
GTGAAATGGGTGCATAAGAATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAG	5	0.125	No Hit
AGTTACAGAAGCGACCCCATAGGCTTTCGCTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAAATCCTTGGTTT	5	0.125	No Hit
GCCGTTGTATAGCCATTCATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGT	5	0.125	No Hit
GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGACGCATACCCAGACGGAAACTAAGTTCCCAC	5	0.125	No Hit
CTAATACCTAAAGCAGTAAACCAAATACCTACTACCGGCCAAGCCGCTAAGAAGAAATGTAAAGAACGAGAATTG	5	0.125	No Hit
TATAGCCATTCATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTAGGAATA	5	0.125	No Hit
CATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTAGGAATAATGGCACCGG	5	0.125	No Hit
GCACTAAAAAGGGAGCCGCCGAATACACCAGCTACACCTAACATGTGAAATGGGTGCATAAGAATGTTGTGCTCA	5	0.125	No Hit
CAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTAGGAATAATGGCACCGGAAA	5	0.125	No Hit
GGTAGGGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAGTGCTAGTTATCCAGTTACAGAAGCG	5	0.125	No Hit
AAGGAACCATGCATAGCACTAAAAAGGGAGCCGCCGAATACACCAGCTACACCTAACATGTGAAATGGGTGCATA	5	0.125	No Hit
GACGCATACCCAGACGGAAACTAAGTTCCCACTCACGACCCATATAACAAGCTACACCAAGTAAAAAGTGTAGAA	5	0.125	No Hit
GCTAAGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAAAATCAATCGGCCAAAATAACCGTGA	5	0.125	No Hit
ATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCCTGACCAATTGGAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.025	0.0	0.0	0.0	0.0
57	0.025	0.0	0.0	0.0	0.0
58	0.025	0.0	0.0	0.0	0.0
59	0.05	0.0	0.0	0.0	0.0
60	0.075	0.0	0.0	0.0	0.0
61	0.075	0.0	0.0	0.0	0.0
62	0.125	0.0	0.0	0.0	0.0
63	0.175	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR30508881 read2 length is 75 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR30508881_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	75
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.9865	32.0	32.0	32.0	27.0	32.0
2	31.123	32.0	32.0	32.0	32.0	32.0
3	35.859	37.0	37.0	37.0	32.0	37.0
4	36.0615	37.0	37.0	37.0	37.0	37.0
5	36.35375	37.0	37.0	37.0	37.0	37.0
6	40.23425	42.0	42.0	42.0	37.0	42.0
7	40.1485	42.0	42.0	42.0	37.0	42.0
8	40.674	42.0	42.0	42.0	37.0	42.0
9	40.748	42.0	42.0	42.0	37.0	42.0
10	40.99175	42.0	42.0	42.0	37.0	42.0
11	40.93475	42.0	42.0	42.0	37.0	42.0
12	40.893	42.0	42.0	42.0	37.0	42.0
13	40.506	42.0	42.0	42.0	37.0	42.0
14	40.4005	42.0	42.0	42.0	37.0	42.0
15	40.6725	42.0	42.0	42.0	37.0	42.0
16	40.8115	42.0	42.0	42.0	37.0	42.0
17	40.58575	42.0	42.0	42.0	37.0	42.0
18	40.311	42.0	42.0	42.0	37.0	42.0
19	40.678	42.0	42.0	42.0	37.0	42.0
20	40.401	42.0	42.0	42.0	37.0	42.0
21	40.7675	42.0	42.0	42.0	37.0	42.0
22	40.723	42.0	42.0	42.0	37.0	42.0
23	40.483	42.0	42.0	42.0	37.0	42.0
24	40.66125	42.0	42.0	42.0	37.0	42.0
25	39.89275	42.0	42.0	42.0	37.0	42.0
26	40.55525	42.0	42.0	42.0	37.0	42.0
27	40.667	42.0	42.0	42.0	37.0	42.0
28	40.50475	42.0	42.0	42.0	37.0	42.0
29	40.3605	42.0	42.0	42.0	37.0	42.0
30	40.24775	42.0	42.0	42.0	37.0	42.0
31	40.3185	42.0	42.0	42.0	37.0	42.0
32	40.4265	42.0	42.0	42.0	37.0	42.0
33	40.58775	42.0	42.0	42.0	37.0	42.0
34	40.69575	42.0	42.0	42.0	37.0	42.0
35	40.66775	42.0	42.0	42.0	37.0	42.0
36	40.46075	42.0	42.0	42.0	37.0	42.0
37	40.187	42.0	42.0	42.0	37.0	42.0
38	40.45825	42.0	42.0	42.0	37.0	42.0
39	40.23525	42.0	42.0	42.0	37.0	42.0
40	40.0105	42.0	42.0	42.0	37.0	42.0
41	40.01675	42.0	42.0	42.0	37.0	42.0
42	40.366	42.0	42.0	42.0	37.0	42.0
43	39.95675	42.0	42.0	42.0	37.0	42.0
44	40.546	42.0	42.0	42.0	37.0	42.0
45	40.3285	42.0	42.0	42.0	37.0	42.0
46	40.43725	42.0	42.0	42.0	37.0	42.0
47	40.35675	42.0	42.0	42.0	37.0	42.0
48	40.12025	42.0	42.0	42.0	37.0	42.0
49	40.2275	42.0	42.0	42.0	37.0	42.0
50	39.7695	42.0	42.0	42.0	37.0	42.0
51	40.05	42.0	42.0	42.0	37.0	42.0
52	40.134	42.0	42.0	42.0	37.0	42.0
53	40.26125	42.0	42.0	42.0	37.0	42.0
54	40.24	42.0	42.0	42.0	37.0	42.0
55	40.16825	42.0	42.0	42.0	37.0	42.0
56	40.015	42.0	42.0	42.0	37.0	42.0
57	40.411	42.0	42.0	42.0	37.0	42.0
58	39.79325	42.0	42.0	42.0	37.0	42.0
59	39.59775	42.0	42.0	42.0	32.0	42.0
60	39.7545	42.0	42.0	42.0	37.0	42.0
61	39.8175	42.0	42.0	42.0	37.0	42.0
62	40.0705	42.0	42.0	42.0	37.0	42.0
63	39.88625	42.0	42.0	42.0	37.0	42.0
64	39.99575	42.0	42.0	42.0	37.0	42.0
65	39.782	42.0	42.0	42.0	37.0	42.0
66	40.0745	42.0	42.0	42.0	37.0	42.0
67	40.1015	42.0	42.0	42.0	37.0	42.0
68	40.09175	42.0	42.0	42.0	37.0	42.0
69	40.248	42.0	42.0	42.0	37.0	42.0
70	39.6515	42.0	42.0	42.0	32.0	42.0
71	39.651	42.0	42.0	42.0	37.0	42.0
72	39.27875	42.0	42.0	42.0	32.0	42.0
73	39.601	42.0	42.0	42.0	32.0	42.0
74	39.321	42.0	42.0	42.0	32.0	42.0
75	39.74875	42.0	42.0	42.0	37.0	42.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
1101	52	0.0
1101	53	0.0
1101	54	0.0
1101	55	0.0
1101	56	0.0
1101	57	0.0
1101	58	0.0
1101	59	0.0
1101	60	0.0
1101	61	0.0
1101	62	0.0
1101	63	0.0
1101	64	0.0
1101	65	0.0
1101	66	0.0
1101	67	0.0
1101	68	0.0
1101	69	0.0
1101	70	0.0
1101	71	0.0
1101	72	0.0
1101	73	0.0
1101	74	0.0
1101	75	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	0.0
19	1.0
20	5.0
21	3.0
22	3.0
23	10.0
24	7.0
25	3.0
26	10.0
27	10.0
28	22.0
29	14.0
30	22.0
31	34.0
32	42.0
33	39.0
34	62.0
35	66.0
36	91.0
37	124.0
38	184.0
39	316.0
40	770.0
41	2160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.375	17.525	18.7	24.4
2	29.15	22.375	31.3	17.175
3	24.4	28.175	31.125000000000004	16.3
4	23.849999999999998	35.75	22.0	18.4
5	24.6	32.125	25.25	18.025
6	19.5	33.2	28.675	18.625
7	18.3	16.35	45.074999999999996	20.275000000000002
8	21.675	21.0	29.599999999999998	27.725
9	22.5	24.0	31.775	21.725
10	24.099999999999998	33.5	26.224999999999998	16.175
11	26.325	22.875	26.025	24.775
12	25.874999999999996	18.825	30.425	24.875
13	24.325	23.275000000000002	32.5	19.900000000000002
14	21.05	24.55	32.725	21.675
15	20.225	25.95	33.7	20.125
16	21.8	25.4	33.4	19.400000000000002
17	27.750000000000004	24.575	28.65	19.025
18	23.599999999999998	25.75	31.275	19.375
19	24.7	26.6	29.25	19.45
20	24.925	26.724999999999998	29.25	19.1
21	22.525000000000002	25.8	31.0	20.674999999999997
22	21.349999999999998	26.174999999999997	32.4	20.075000000000003
23	21.65	24.95	32.824999999999996	20.575
24	21.75	24.625	31.674999999999997	21.95
25	22.175	26.05	30.5	21.275
26	22.075	26.150000000000002	32.25	19.525000000000002
27	22.900000000000002	26.85	30.575000000000003	19.675
28	21.25	27.825	30.975	19.950000000000003
29	21.675	26.424999999999997	31.8	20.1
30	22.3	27.05	32.45	18.2
31	21.825	28.299999999999997	29.725	20.150000000000002
32	22.75	24.075	33.900000000000006	19.275000000000002
33	21.275	26.775	31.924999999999997	20.025000000000002
34	20.5	27.925	32.2	19.375
35	23.75	27.85	29.975	18.425
36	21.975	28.1	30.8	19.125
37	22.225	27.200000000000003	29.7	20.875
38	22.95	27.224999999999998	30.075000000000003	19.75
39	21.3	28.599999999999998	31.05	19.05
40	21.75	27.375	31.5	19.375
41	22.650000000000002	24.825	30.825000000000003	21.7
42	20.349999999999998	27.85	30.099999999999998	21.7
43	22.275	26.85	32.0	18.875
44	24.325	27.500000000000004	29.975	18.2
45	23.525	28.349999999999998	29.95	18.175
46	22.3	27.224999999999998	30.625000000000004	19.85
47	22.400000000000002	26.150000000000002	31.3	20.150000000000002
48	21.5	25.424999999999997	33.074999999999996	20.0
49	21.05	24.5	33.575	20.875
50	23.25	24.474999999999998	32.425	19.85
51	23.549999999999997	25.2	31.374999999999996	19.875
52	21.05	27.575	30.375000000000004	21.0
53	21.425	24.825	31.4	22.35
54	23.05	25.5	29.95	21.5
55	23.549999999999997	24.6	30.4	21.45
56	23.775	24.125	31.974999999999998	20.125
57	21.4	25.2	32.925	20.474999999999998
58	22.125	25.85	31.674999999999997	20.349999999999998
59	22.3	23.95	31.974999999999998	21.775
60	22.75	23.599999999999998	32.85	20.8
61	23.175	24.325	31.424999999999997	21.075
62	21.125	25.25	31.125000000000004	22.5
63	20.4	28.825	30.075000000000003	20.7
64	21.575	26.400000000000002	30.725	21.3
65	21.625	25.650000000000002	33.575	19.15
66	22.425	26.924999999999997	31.65	19.0
67	20.8	25.575	34.525	19.1
68	20.75	27.05	31.900000000000002	20.3
69	24.525	26.125	30.4	18.95
70	23.200000000000003	26.950000000000003	30.15	19.7
71	23.45	26.974999999999998	28.475	21.099999999999998
72	23.525	28.000000000000004	30.525000000000002	17.95
73	25.674999999999997	26.150000000000002	28.375	19.8
74	24.099999999999998	27.400000000000002	27.525	20.974999999999998
75	22.7	27.025	31.874999999999996	18.4
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	1.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	0.5
16	0.0
17	1.0
18	1.0
19	1.0
20	2.0
21	2.5
22	6.5
23	8.0
24	6.0
25	6.5
26	9.0
27	12.5
28	14.0
29	22.0
30	30.0
31	40.0
32	50.0
33	58.5
34	79.0
35	134.0
36	177.0
37	247.5
38	334.0
39	348.5
40	347.0
41	341.0
42	323.5
43	326.5
44	341.0
45	331.0
46	318.5
47	250.0
48	184.0
49	152.0
50	128.0
51	121.0
52	106.0
53	81.5
54	55.5
55	57.0
56	60.0
57	48.5
58	34.5
59	27.5
60	23.0
61	22.5
62	18.5
63	11.0
64	7.0
65	4.5
66	1.0
67	0.0
68	0.0
69	0.0
70	0.5
71	1.0
72	1.0
73	0.5
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.5
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.53237410071942	58.75
2	7.913669064748201	11.0
3	2.76978417266187	5.775
4	1.4028776978417266	3.9
5	0.935251798561151	3.25
6	0.5035971223021583	2.1
7	0.539568345323741	2.625
8	0.2517985611510791	1.4000000000000001
9	0.2158273381294964	1.35
>10	0.8992805755395683	8.5
>50	0.03597122302158273	1.35
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGAATTTGTGCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAGA	54	1.35	No Hit
AGAATTTGTGCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAGAC	46	1.15	No Hit
CGTGAACCTGTTTCTGGATCTCTTCTTTACGGAAACAATATTATTTCCGGTGCCATTATTCCTACTTCTGCAGCT	18	0.44999999999999996	No Hit
GCGAAAGCGAAAGCCTATGGGGTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGTCTTTACATTGGATGGT	17	0.42500000000000004	No Hit
GTTAGGTGTAGCTGGTGTATTCGGCGGCTCCCTTTTTAGTGCTATGCATGGTTCCTTGGTAACTTCTAGTTTGAT	17	0.42500000000000004	No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTTTATGATTGTATTCCAGGCTGAGCACAACA	16	0.4	No Hit
AATTTGTGCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAGACGC	15	0.375	No Hit
GTTTGATCAGGGAAACCACAGAAAATGAATCTGCTAATGAAGGTTACAGATTCGGGCAAGAAGAAGAAACTTACA	14	0.35000000000000003	No Hit
TCTACTTAGAGAATTTGTGCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTT	13	0.325	No Hit
TTTCAACCTAAATGGTTTCAATTTCAACCAATCAGTAGTTGATAGTCAAGGACGTGTTATTAATACTTGGGCTGA	13	0.325	No Hit
GTGCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAGACGCGAAAG	13	0.325	No Hit
GCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAGACGCGAAAGCG	13	0.325	No Hit
AGAAAATGAATCTGCTAATGAAGGTTACAGATTCGGGCAAGAAGAAGAAACTTACAACATTGTAGCTGCTCACGG	12	0.3	No Hit
GCTGCGACTGCTGTTTTCTTGATCTATCCAATTGGTCAGGGAAGTTTTTCTGATGGTATGCCTCTAGGAATCTCT	11	0.27499999999999997	No Hit
CTGCGACTGCTGTTTTCTTGATCTATCCAATTGGTCAGGGAAGTTTTTCTGATGGTATGCCTCTAGGAATCTCTG	11	0.27499999999999997	No Hit
GGAATCTCTGGTACTTTCAACTTTATGATTGTATTCCAGGCTGAGCACAACATTCTTATGCACCCATTTCACATG	11	0.27499999999999997	No Hit
TGATAGTCAAGGACGTGTTATTAATACTTGGGCTGATATTATTAACCGTGCTAACCTTGGTATGGAAGTTATGCA	10	0.25	No Hit
GCGAAAGCCTATGGGGTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGTCTTTACATTGGATGGTTTGGTG	10	0.25	No Hit
CAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATCTATCCAATTGGTCAGGGAAGTTTTTCTGATGGTATGC	10	0.25	No Hit
TGGCTTTCAACCTAAATGGTTTCAATTTCAACCAATCAGTAGTTGATAGTCAAGGACGTGTTATTAATACTTGGG	10	0.25	No Hit
GTTTTCTTGATCTATCCAATTGGTCAGGGAAGTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAAC	10	0.25	No Hit
GGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTG	10	0.25	No Hit
GGAAGTTATGCATGAACGTAATGCTCACAACTTCCCTCTAGACCTAGCTGCTGTTGAGGCTCCATCTACAAATGG	10	0.25	No Hit
AAGGATTTTACCATGACTGCAATTTTAGAGAGACGCGAAAGCGAAAGCCTATGGGGTCGCTTCTGTAACTGGATA	10	0.25	No Hit
TCTTGATCTATCCAATTGGTCAGGGAAGTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTTTA	10	0.25	No Hit
GGCGGCTCCCTTTTTAGTGCTATGCATGGTTCCTTGGTAACTTCTAGTTTGATCAGGGAAACCACAGAAAATGAA	10	0.25	No Hit
AGAGAATTTGTGCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAG	9	0.22499999999999998	No Hit
CAAGGATTTTACCATGACTGCAATTTTAGAGAGACGCGAAAGCGAAAGCCTATGGGGTCGCTTCTGTAACTGGAT	9	0.22499999999999998	No Hit
GCACAACATTCTTATGCACCCATTTCACATGTTAGGTGTAGCTGGTGTATTCGGCGGCTCCCTTTTTAGTGCTAT	9	0.22499999999999998	No Hit
CTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATCTATCCAATTGGTCAGG	9	0.22499999999999998	No Hit
TGAAAACCGTCTTTACATTGGATGGTTTGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTTTTTAT	9	0.22499999999999998	No Hit
CCGGTAGTAGGTATTTGGTTTACTGCTTTAGGTATTAGTACTATGGCTTTCAACCTAAATGGTTTCAATTTCAAC	9	0.22499999999999998	No Hit
TGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTTTATGATTGTATTCCAGGCTGAGCACAACATTCTTAT	8	0.2	No Hit
GTAACTTCTAGTTTGATCAGGGAAACCACAGAAAATGAATCTGCTAATGAAGGTTACAGATTCGGGCAAGAAGAA	8	0.2	No Hit
CTGGATAACTAGCACTGAAAACCGTCTTTACATTGGATGGTTTGGTGTTTTGATGATCCCTACCTTATTGACCGC	8	0.2	No Hit
GTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGTCTTTACATTGGATGGTTTGGTGTTTTGATGATCCCTA	8	0.2	No Hit
GGTACTTTCAACTTTATGATTGTATTCCAGGCTGAGCACAACATTCTTATGCACCCATTTCACATGTTAGGTGTA	8	0.2	No Hit
GAAAATGAATCTGCTAATGAAGGTTACAGATTCGGGCAAGAAGAAGAAACTTACAACATTGTAGCTGCTCACGGT	8	0.2	No Hit
GCTAGTTTCAACAATTCTCGTTCTTTACATTTCTTCTTAGCGGCTTGGCCGGTAGTAGGTATTTGGTTTACTGCT	8	0.2	No Hit
CTTTTTAGTGCTATGCATGGTTCCTTGGTAACTTCTAGTTTGATCAGGGAAACCACAGAAAATGAATCTGCTAAT	7	0.17500000000000002	No Hit
GTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCT	7	0.17500000000000002	No Hit
GCAGCTGCGACTGCTGTTTTCTTGATCTATCCAATTGGTCAGGGAAGTTTTTCTGATGGTATGCCTCTAGGAATC	7	0.17500000000000002	No Hit
TTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATCTATCCAATTGGTCAGGGAAGTTTTTCTGATGGTAT	7	0.17500000000000002	No Hit
GAATTTGTGCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAGACG	7	0.17500000000000002	No Hit
ATGGAAGTTATGCATGAACGTAATGCTCACAACTTCCCTCTAGACCTAGCTGCTGTTGAGGCTCCATCTACAAAT	7	0.17500000000000002	No Hit
TGCTAATGAAGGTTACAGATTCGGGCAAGAAGAAGAAACTTACAACATTGTAGCTGCTCACGGTTATTTTGGCCG	7	0.17500000000000002	No Hit
GGAAACCACAGAAAATGAATCTGCTAATGAAGGTTACAGATTCGGGCAAGAAGAAGAAACTTACAACATTGTAGC	7	0.17500000000000002	No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGAC	7	0.17500000000000002	No Hit
TATTAACCGTGCTAACCTTGGTATGGAAGTTATGCATGAACGTAATGCTCACAACTTCCCTCTAGACCTAGCTGC	7	0.17500000000000002	No Hit
CAACATTCTTATGCACCCATTTCACATGTTAGGTGTAGCTGGTGTATTCGGCGGCTCCCTTTTTAGTGCTATGCA	7	0.17500000000000002	No Hit
TTTACATTGGATGGTTTGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTTTTTATTATCGCATTCA	7	0.17500000000000002	No Hit
CTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAGACGCGAAAGCGAAA	7	0.17500000000000002	No Hit
GTGCTATGCATGGTTCCTTGGTAACTTCTAGTTTGATCAGGGAAACCACAGAAAATGAATCTGCTAATGAAGGTT	7	0.17500000000000002	No Hit
GCGACTGCTGTTTTCTTGATCTATCCAATTGGTCAGGGAAGTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGT	7	0.17500000000000002	No Hit
CGGCGGTCCTTATGAACTAATTGTTCTACACTTTTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGAACT	6	0.15	No Hit
TATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATCTATCCAAT	6	0.15	No Hit
GTCGTGAGTGGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAG	6	0.15	No Hit
GTGTATTCGGCGGCTCCCTTTTTAGTGCTATGCATGGTTCCTTGGTAACTTCTAGTTTGATCAGGGAAACCACAG	6	0.15	No Hit
GGATTTTACCATGACTGCAATTTTAGAGAGACGCGAAAGCGAAAGCCTATGGGGTCGCTTCTGTAACTGGATAAC	6	0.15	No Hit
GGCGGTCCTTATGAACTAATTGTTCTACACTTTTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGAACTT	6	0.15	No Hit
CGGGCAAGAAGAAGAAACTTACAACATTGTAGCTGCTCACGGTTATTTTGGCCGATTGATTTTCCAATATGCTAG	6	0.15	No Hit
GCTAATGAAGGTTACAGATTCGGGCAAGAAGAAGAAACTTACAACATTGTAGCTGCTCACGGTTATTTTGGCCGA	6	0.15	No Hit
CATGACTGCAATTTTAGAGAGACGCGAAAGCGAAAGCCTATGGGGTCGCTTCTGTAACTGGATAACTAGCACTGA	6	0.15	No Hit
GTGAACCTGTTTCTGGATCTCTTCTTTACGGAAACAATATTATTTCCGGTGCCATTATTCCTACTTCTGCAGCTA	6	0.15	No Hit
CAACATTGTAGCTGCTCACGGTTATTTTGGCCGATTGATTTTCCAATATGCTAGTTTCAACAATTCTCGTTCTTT	6	0.15	No Hit
GGAAGCTGCATCCGTTGATGAATGGCTATACAACGGCGGTCCTTATGAACTAATTGTTCTACACTTTTTACTTGG	6	0.15	No Hit
GTACTTTCAACTTTATGATTGTATTCCAGGCTGAGCACAACATTCTTATGCACCCATTTCACATGTTAGGTGTAG	6	0.15	No Hit
ATTTGGTTTACTGCTTTAGGTATTAGTACTATGGCTTTCAACCTAAATGGTTTCAATTTCAACCAATCAGTAGTT	6	0.15	No Hit
AATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAGACGCGAAAGCGAAAGCCTATGGGGTCGCTTCTGTA	5	0.125	No Hit
AAAATGAATCTGCTAATGAAGGTTACAGATTCGGGCAAGAAGAAGAAACTTACAACATTGTAGCTGCTCACGGTT	5	0.125	No Hit
TTTCAACTTTATGATTGTATTCCAGGCTGAGCACAACATTCTTATGCACCCATTTCACATGTTAGGTGTAGCTGG	5	0.125	No Hit
ACAACATTGTAGCTGCTCACGGTTATTTTGGCCGATTGATTTTCCAATATGCTAGTTTCAACAATTCTCGTTCTT	5	0.125	No Hit
GCAATTTTAGAGAGACGCGAAAGCGAAAGCCTATGGGGTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGT	5	0.125	No Hit
TCCTACTTCTGCAGCTATTGGATTGCATTTTTACCCAATCTGGGAAGCTGCATCCGTTGATGAATGGCTATACAA	5	0.125	No Hit
CGAAAGCGAAAGCCTATGGGGTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGTCTTTACATTGGATGGTT	5	0.125	No Hit
GCTGCATCCGTTGATGAATGGCTATACAACGGCGGTCCTTATGAACTAATTGTTCTACACTTTTTACTTGGTGTA	5	0.125	No Hit
TTTCAACCAATCAGTAGTTGATAGTCAAGGACGTGTTATTAATACTTGGGCTGATATTATTAACCGTGCTAACCT	5	0.125	No Hit
CTGCTAATGAAGGTTACAGATTCGGGCAAGAAGAAGAAACTTACAACATTGTAGCTGCTCACGGTTATTTTGGCC	5	0.125	No Hit
CGGCGGCTCCCTTTTTAGTGCTATGCATGGTTCCTTGGTAACTTCTAGTTTGATCAGGGAAACCACAGAAAATGA	5	0.125	No Hit
GCTATACAACGGCGGTCCTTATGAACTAATTGTTCTACACTTTTTACTTGGTGTAGCTTGTTATATGGGTCGTGA	5	0.125	No Hit
AGCACTGAAAACCGTCTTTACATTGGATGGTTTGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTT	5	0.125	No Hit
AACATTCTTATGCACCCATTTCACATGTTAGGTGTAGCTGGTGTATTCGGCGGCTCCCTTTTTAGTGCTATGCAT	5	0.125	No Hit
GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATCTATCCAATTGGTCAGGGAAGTTTTTCT	5	0.125	No Hit
GCATTTTTACCCAATCTGGGAAGCTGCATCCGTTGATGAATGGCTATACAACGGCGGTCCTTATGAACTAATTGT	5	0.125	No Hit
GCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATCTATCCAATTGG	5	0.125	No Hit
TGGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACT	5	0.125	No Hit
GGTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGTCTTTACATTGGATGGTTTGGTGTTTTGATGATCCCT	5	0.125	No Hit
GAAGAAACTTACAACATTGTAGCTGCTCACGGTTATTTTGGCCGATTGATTTTCCAATATGCTAGTTTCAACAAT	5	0.125	No Hit
GGTGTAGCTGGTGTATTCGGCGGCTCCCTTTTTAGTGCTATGCATGGTTCCTTGGTAACTTCTAGTTTGATCAGG	5	0.125	No Hit
CCTACTTCTGCAGCTATTGGATTGCATTTTTACCCAATCTGGGAAGCTGCATCCGTTGATGAATGGCTATACAAC	5	0.125	No Hit
TCTGGATCTCTTCTTTACGGAAACAATATTATTTCCGGTGCCATTATTCCTACTTCTGCAGCTATTGGATTGCAT	5	0.125	No Hit
TTCCAATATGCTAGTTTCAACAATTCTCGTTCTTTACATTTCTTCTTAGCGGCTTGGCCGGTAGTAGGTATTTGG	5	0.125	No Hit
GCTGCTCCTCCAGTAGATATTGATGGTATTCGTGAACCTGTTTCTGGATCTCTTCTTTACGGAAACAATATTATT	5	0.125	No Hit
AGAAGAAGAAACTTACAACATTGTAGCTGCTCACGGTTATTTTGGCCGATTGATTTTCCAATATGCTAGTTTCAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.025	0.0	0.0	0.0	0.0
57	0.025	0.0	0.0	0.0	0.0
58	0.025	0.0	0.0	0.0	0.0
59	0.05	0.0	0.0	0.0	0.0
60	0.075	0.0	0.0	0.0	0.0
61	0.075	0.0	0.0	0.0	0.0
62	0.125	0.0	0.0	0.0	0.0
63	0.175	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 21192978 spots for SRR30508881.sra
Written 21192978 spots for SRR30508881.sra
Read 21192978 spots for SRR30508881.sra
Written 21192978 spots for SRR30508881.sra
Read 21192978 spots for SRR30508881.sra
Written 21192978 spots for SRR30508881.sra
Read 21192982 spots for SRR30508881.sra
Written 21192982 spots for SRR30508881.sra
Read 21192978 spots for SRR30508881.sra
Written 21192978 spots for SRR30508881.sra
Read 21192978 spots for SRR30508881.sra
Written 21192978 spots for SRR30508881.sra
SRR ids: ['SRR30508881.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_nu3vqqfh
SRR30508881.sra spots: 127157872
blocks: [[1, 21192978], [21192979, 42385956], [42385957, 63578934], [63578935, 84771912], [84771913, 105964890], [105964891, 127157872]]
SRR30508881 file size 28313936
SRR30508881 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 6 -o SRR30508881 SRR30508881_1.fastq SRR30508881_2.fastq
Input file:	SRR30508881_1.fastq
Paired file:	SRR30508881_2.fastq
trimmed:	SRR30508881-trimmed-pair1.fastq, SRR30508881-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	6
Thu Oct 16 12:56:24 2025 >> started

Thu Oct 16 13:05:06 2025 >> done (522.016s)
127157872 read pairs processed; of these:
    20408 ( 0.02%) short read pairs filtered out after trimming by size control
   119563 ( 0.09%) empty read pairs filtered out after trimming by size control
127017901 (99.89%) read pairs available; of these:
   817211 ( 0.64%) trimmed read pairs available after processing
126200690 (99.36%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      130	  0.00%
 19	      136	  0.00%
 20	      161	  0.00%
 21	      183	  0.00%
 22	      217	  0.00%
 23	      238	  0.00%
 24	      237	  0.00%
 25	      360	  0.00%
 26	      264	  0.00%
 27	      302	  0.00%
 28	      346	  0.00%
 29	      344	  0.00%
 30	      458	  0.00%
 31	      421	  0.00%
 32	      573	  0.00%
 33	      535	  0.00%
 34	      633	  0.00%
 35	     1265	  0.00%
 36	     1344	  0.00%
 37	     1588	  0.00%
 38	     1766	  0.00%
 39	     1906	  0.00%
 40	     2122	  0.00%
 41	     2636	  0.00%
 42	     2943	  0.00%
 43	     3146	  0.00%
 44	     3491	  0.00%
 45	     3957	  0.00%
 46	     4394	  0.00%
 47	     5427	  0.00%
 48	     6513	  0.01%
 49	     8245	  0.01%
 50	     9274	  0.01%
 51	    11145	  0.01%
 52	    13078	  0.01%
 53	    13270	  0.01%
 54	    13319	  0.01%
 55	    14856	  0.01%
 56	    15541	  0.01%
 57	    17694	  0.01%
 58	    20727	  0.02%
 59	    22520	  0.02%
 60	    26227	  0.02%
 61	    29567	  0.02%
 62	    33961	  0.03%
 63	    35153	  0.03%
 64	    40084	  0.03%
 65	    41031	  0.03%
 66	    42220	  0.03%
 67	    46942	  0.04%
 68	    52181	  0.04%
 69	    62089	  0.05%
 70	    67443	  0.05%
 71	    75637	  0.06%
 72	    86908	  0.07%
 73	    96349	  0.08%
 74	   191346	  0.15%
 75	 94378552	 74.30%
 76	    51223	  0.04%
 77	   260487	  0.21%
 78	  2463826	  1.94%
 79	 15775629	 12.42%
 80	 12953341	 10.20%
127017901 reads passed initial QC


criterion=sequence-density
sequence-density=0.32
sequence-density-rank=1
fanout-score=4.53
fanout-score-rank=11
prefix-density=0.73
prefix-fanout=2.0
sequence=AGCGGGAGGTGATCGCTGCTCTGTGAAACCAGCCTCACGCATTGCCCTCCACTGCAAGAACCGCCTGAGCCAAGCCTTCGCTTAACACACACGGATCACTCTCAGCTTGGACTGGGCAGCCCGGAACAATGCCACAACAGATCCGACCCGTGGGCCGAGCCAGTGTTCCACCAACTTATTGTCCCCACACGGGTCAC


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=30
fanout-score=123.34
fanout-score-rank=1
prefix-density=1.85
prefix-fanout=1.0
sequence=CGGTTAATAATCCCGCTGGTACTGAAACCATTAAAAGAACACCCAATAATTTGTTAGGCACTGTACGAAGTATTTGAAATACAGGAAAGAAATACCATTCAGGTAATATTTCCAAAGGAGTTGCAAAAGGATCCGCAGGTTCACCAATCATTGATGGTTCTAAAACCGCTAAGCCTACGTTACAGGCAATGGTACCAAGAATAACTACTGGAAAAATATATAAAAGGTCGTTGGGCCATGCGGGTTCCCCGTAATAATTGTGACCCATACCTTTAGCTAATTTAGCTCGTAATACAGGATCATTCAAATCTGGTTTTTTTGTTACTCCCATAATCCATTATCTCTTCATAGAATTCCCTTCAACTTTTTATGTCAAAAAAATAATAAAATATTGTGGAGTTGAAGGGAAATATCCAAATACATGTTTTATTTTTTTAATAATCCATATTTATAGCAATAAAATACTATCGTTCCTTCACCAAGTAATAAGTAAGTACAAATACCTAGAATC


criterion=sequence-density
sequence-density=0.36
sequence-density-rank=1
fanout-score=2.11
fanout-score-rank=24
prefix-density=0.40
prefix-fanout=1.9
sequence=CACCTCCCGCTCT


criterion=fanout-score
sequence-density=0.05
sequence-density-rank=18
fanout-score=66.16
fanout-score-rank=1
prefix-density=3.19
prefix-fanout=1.0
sequence=CTCCCTTTTTTA
SRR30508881 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Oct 16 13:05:32
                             Started mapping on |	Oct 16 13:05:32
                                    Finished on |	Oct 16 13:22:28
       Mapping speed, Million of reads per hour |	450.06

                          Number of input reads |	127017901
                      Average input read length |	152
                                    UNIQUE READS:
                   Uniquely mapped reads number |	115936502
                        Uniquely mapped reads % |	91.28%
                          Average mapped length |	151.94
                       Number of splices: Total |	20430172
            Number of splices: Annotated (sjdb) |	20293500
                       Number of splices: GT/AG |	19968740
                       Number of splices: GC/AG |	195771
                       Number of splices: AT/AC |	155682
               Number of splices: Non-canonical |	109979
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.32
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.09
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2754635
             % of reads mapped to multiple loci |	2.17%
        Number of reads mapped to too many loci |	4918
             % of reads mapped to too many loci |	0.00%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.55%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	8328459	8328459	8328459
N_multimapping	2754635	2754635	2754635
N_noFeature	2693702	111294967	4740051
N_ambiguous	4711398	53685	2093237
UnstrandedReadsAssigned:108531402 PositiveStrandReadsAssigned:4587850 NegativeStrandReadsAssigned:109103214
Dataset is classified negative stranded
MeadianReadLen=75 20thPercentileLength=75 echo kmer=71
SRR30508881 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 48,359
[index] number of k-mers: 45,567,218
[index] number of equivalence classes: 93,973
[quant] running in paired-end mode
[quant] will process pair 1: SRR30508881-trimmed-pair1.fastq
                             SRR30508881-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 127,017,901 reads, 112,317,502 reads pseudoaligned
[quant] estimated average fragment length: 161.019
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,334 rounds

  48360 SRR30508881.ke.tsv
  32833 SRR30508881.se.tsv
  81193 total
==> SRR30508881.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AT1G19090.1	2509	2348.98	20	0.0566367
AT1G18320.1	699	540.242	2.73208	0.0336397
AT5G11100.1	1709	1548.98	244	1.04783
AT4G35335.1	1280	1119.98	461	2.73803
AT1G60930.1	3876	3715.98	362	0.648011
AT1G17000.1	2351	2190.98	0	0
AT3G55270.1	3130	2969.98	2638	5.90838
AT4G16150.1	3181	3020.98	2432	5.35504
AT3G18150.1	1370	1209.98	0	0

==> SRR30508881.se.tsv <==
AT1G01010	691
AT1G01020	239
AT1G03987	0
AT1G01030	672
AT1G01040	1325
AT1G03993	3
AT1G01046	8
AT1G01050	2535
AT1G03997	0
AT1G01060	6694
SRR30508881 completed mapping pipeline successfully
