Starting /dee2/code/volunteer_pipeline.sh SRR30509053
    current disk space = 1568336704
    free memory = 55873024 
SRR30509053 SRAfilesize
547f95343d9c280fe1fccd9e2bb8340e  SRR30509053.sra
SRR30509053.sra file validated
SRR30509053 is paired end
SRR30509053 is conventional basespace
SRR30509053 read1 length is 75 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR30509053_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	75
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.302	32.0	32.0	32.0	32.0	32.0
2	31.713	32.0	32.0	32.0	32.0	32.0
3	35.88925	37.0	37.0	37.0	32.0	37.0
4	36.56025	37.0	37.0	37.0	37.0	37.0
5	36.655	37.0	37.0	37.0	37.0	37.0
6	40.99325	42.0	42.0	42.0	37.0	42.0
7	41.0295	42.0	42.0	42.0	37.0	42.0
8	41.18825	42.0	42.0	42.0	37.0	42.0
9	41.20925	42.0	42.0	42.0	37.0	42.0
10	41.25075	42.0	42.0	42.0	42.0	42.0
11	41.2905	42.0	42.0	42.0	42.0	42.0
12	41.1665	42.0	42.0	42.0	42.0	42.0
13	40.98125	42.0	42.0	42.0	37.0	42.0
14	41.204	42.0	42.0	42.0	37.0	42.0
15	41.297	42.0	42.0	42.0	42.0	42.0
16	41.294	42.0	42.0	42.0	42.0	42.0
17	41.3295	42.0	42.0	42.0	42.0	42.0
18	41.29325	42.0	42.0	42.0	42.0	42.0
19	40.82475	42.0	42.0	42.0	37.0	42.0
20	41.12525	42.0	42.0	42.0	37.0	42.0
21	41.01675	42.0	42.0	42.0	37.0	42.0
22	41.0495	42.0	42.0	42.0	37.0	42.0
23	41.083	42.0	42.0	42.0	37.0	42.0
24	40.87325	42.0	42.0	42.0	37.0	42.0
25	41.06075	42.0	42.0	42.0	37.0	42.0
26	40.733	42.0	42.0	42.0	37.0	42.0
27	40.58325	42.0	42.0	42.0	37.0	42.0
28	40.51875	42.0	42.0	42.0	37.0	42.0
29	40.742	42.0	42.0	42.0	37.0	42.0
30	40.64475	42.0	42.0	42.0	37.0	42.0
31	40.7865	42.0	42.0	42.0	37.0	42.0
32	40.7085	42.0	42.0	42.0	37.0	42.0
33	40.521	42.0	42.0	42.0	37.0	42.0
34	40.652	42.0	42.0	42.0	37.0	42.0
35	40.75125	42.0	42.0	42.0	37.0	42.0
36	40.2595	42.0	42.0	42.0	37.0	42.0
37	40.3975	42.0	42.0	42.0	37.0	42.0
38	40.2825	42.0	42.0	42.0	37.0	42.0
39	40.659	42.0	42.0	42.0	37.0	42.0
40	40.67625	42.0	42.0	42.0	37.0	42.0
41	40.66925	42.0	42.0	42.0	37.0	42.0
42	40.3395	42.0	42.0	42.0	37.0	42.0
43	40.6235	42.0	42.0	42.0	37.0	42.0
44	40.3515	42.0	42.0	42.0	37.0	42.0
45	40.45925	42.0	42.0	42.0	37.0	42.0
46	40.6335	42.0	42.0	42.0	37.0	42.0
47	40.77025	42.0	42.0	42.0	37.0	42.0
48	40.55425	42.0	42.0	42.0	37.0	42.0
49	40.59925	42.0	42.0	42.0	37.0	42.0
50	39.642	42.0	42.0	42.0	32.0	42.0
51	40.39775	42.0	42.0	42.0	37.0	42.0
52	40.66675	42.0	42.0	42.0	37.0	42.0
53	40.8	42.0	42.0	42.0	37.0	42.0
54	40.58475	42.0	42.0	42.0	37.0	42.0
55	40.554	42.0	42.0	42.0	37.0	42.0
56	40.86075	42.0	42.0	42.0	37.0	42.0
57	40.69925	42.0	42.0	42.0	37.0	42.0
58	40.6305	42.0	42.0	42.0	37.0	42.0
59	40.6705	42.0	42.0	42.0	37.0	42.0
60	40.7055	42.0	42.0	42.0	37.0	42.0
61	40.549	42.0	42.0	42.0	37.0	42.0
62	40.36225	42.0	42.0	42.0	37.0	42.0
63	40.391	42.0	42.0	42.0	37.0	42.0
64	40.072	42.0	42.0	42.0	37.0	42.0
65	39.951	42.0	42.0	42.0	37.0	42.0
66	40.19525	42.0	42.0	42.0	37.0	42.0
67	39.67	42.0	42.0	42.0	32.0	42.0
68	40.2145	42.0	42.0	42.0	37.0	42.0
69	40.39675	42.0	42.0	42.0	37.0	42.0
70	40.198	42.0	42.0	42.0	37.0	42.0
71	40.007	42.0	42.0	42.0	37.0	42.0
72	40.27	42.0	42.0	42.0	37.0	42.0
73	39.4225	42.0	42.0	42.0	32.0	42.0
74	39.892	42.0	42.0	42.0	37.0	42.0
75	40.38125	42.0	42.0	42.0	37.0	42.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
1101	52	0.0
1101	53	0.0
1101	54	0.0
1101	55	0.0
1101	56	0.0
1101	57	0.0
1101	58	0.0
1101	59	0.0
1101	60	0.0
1101	61	0.0
1101	62	0.0
1101	63	0.0
1101	64	0.0
1101	65	0.0
1101	66	0.0
1101	67	0.0
1101	68	0.0
1101	69	0.0
1101	70	0.0
1101	71	0.0
1101	72	0.0
1101	73	0.0
1101	74	0.0
1101	75	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	0.0
25	2.0
26	4.0
27	4.0
28	6.0
29	9.0
30	24.0
31	25.0
32	35.0
33	47.0
34	68.0
35	72.0
36	97.0
37	108.0
38	151.0
39	234.0
40	565.0
41	2548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.15	19.05	11.600000000000001	36.199999999999996
2	23.125	28.199999999999996	30.599999999999998	18.075
3	19.55	36.199999999999996	20.724999999999998	23.525
4	22.85	37.675	19.05	20.424999999999997
5	20.525	40.325	22.225	16.925
6	17.724999999999998	41.375	21.0	19.900000000000002
7	14.975	22.475	41.425	21.125
8	19.975	27.125	24.675	28.225
9	19.55	26.3	27.450000000000003	26.700000000000003
10	18.6	43.55	19.675	18.175
11	24.85	29.525000000000002	19.0	26.625
12	23.150000000000002	23.225	24.925	28.7
13	19.35	30.5	25.624999999999996	24.525
14	19.5	31.974999999999998	26.724999999999998	21.8
15	20.65	30.95	27.075	21.325
16	19.7	32.35	23.95	24.0
17	20.974999999999998	33.525	26.075	19.425
18	19.925	30.75	26.1	23.225
19	18.873168266801414	33.021728145528044	24.70944921677615	23.39565437089439
20	19.3	32.375	25.224999999999998	23.1
21	18.4	30.85	27.675	23.075000000000003
22	18.65	34.8	24.55	22.0
23	20.925	34.675	24.825	19.575
24	18.625	33.550000000000004	25.1	22.725
25	20.599999999999998	31.0	25.525	22.875
26	18.95	33.25	26.474999999999998	21.325
27	19.05	33.175	25.05	22.725
28	21.4	34.125	23.625	20.849999999999998
29	19.175	32.05	25.874999999999996	22.900000000000002
30	21.425	32.75	24.15	21.675
31	20.424999999999997	33.1	24.55	21.925
32	20.75	32.2	24.975	22.075
33	20.75	33.300000000000004	24.6	21.349999999999998
34	20.0	31.175000000000004	26.875	21.95
35	20.025000000000002	32.425	25.55	22.0
36	21.05	31.525	24.625	22.8
37	22.05	30.475	26.375	21.099999999999998
38	20.05	31.5	26.8	21.65
39	20.549999999999997	30.525000000000002	27.125	21.8
40	20.349999999999998	32.15	26.974999999999998	20.525
41	18.9	33.85	26.375	20.875
42	19.1	32.675	27.125	21.099999999999998
43	20.200000000000003	33.375	24.075	22.35
44	19.75	32.175	26.825	21.25
45	21.55	31.674999999999997	26.825	19.950000000000003
46	17.075000000000003	34.225	26.1	22.6
47	19.875	34.375	24.95	20.8
48	21.475	32.1	24.575	21.85
49	21.125	31.175000000000004	27.35	20.349999999999998
50	20.5	32.05	26.5	20.95
51	20.9	30.4	27.1	21.6
52	20.724999999999998	30.099999999999998	26.75	22.425
53	20.724999999999998	32.25	26.950000000000003	20.075000000000003
54	20.549999999999997	29.925	26.950000000000003	22.575
55	18.475	31.874999999999996	28.625	21.025
56	20.625	30.225	28.000000000000004	21.15
57	20.175	31.474999999999998	24.65	23.7
58	21.25	33.5	24.625	20.625
59	21.8	32.425	25.124999999999996	20.65
60	18.975	32.175	28.050000000000004	20.8
61	21.475	32.225	26.025	20.275000000000002
62	19.625	28.349999999999998	27.375	24.65
63	21.0	29.849999999999998	26.1	23.05
64	20.075000000000003	32.324999999999996	25.525	22.075
65	19.5	34.025	24.95	21.525
66	20.075000000000003	35.3	26.1	18.525
67	19.225	31.525	27.975	21.275
68	21.575	30.5	23.724999999999998	24.2
69	20.825	30.425	24.925	23.825
70	21.5	31.05	25.775	21.675
71	20.474999999999998	31.1	26.75	21.675
72	21.55	29.9	26.05	22.5
73	20.4	32.824999999999996	23.674999999999997	23.1
74	19.55	33.650000000000006	24.675	22.125
75	20.0	32.525	26.5	20.974999999999998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	1.5
16	1.0
17	1.0
18	0.5
19	1.0
20	2.0
21	2.0
22	3.5
23	8.0
24	11.0
25	11.0
26	15.5
27	19.5
28	19.0
29	26.0
30	38.5
31	51.5
32	59.0
33	74.5
34	115.0
35	136.5
36	133.0
37	218.0
38	353.5
39	383.5
40	363.0
41	366.5
42	321.0
43	315.0
44	358.0
45	307.0
46	283.0
47	245.5
48	181.0
49	154.5
50	116.5
51	94.5
52	84.0
53	73.0
54	59.0
55	58.5
56	61.0
57	52.0
58	34.0
59	21.0
60	17.0
61	15.0
62	11.0
63	5.5
64	2.0
65	1.5
66	2.0
67	2.0
68	1.0
69	0.5
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.05
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.2
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.01759530791789	57.3
2	8.431085043988269	11.5
3	2.7126099706744866	5.55
4	1.3929618768328444	3.8
5	0.9530791788856305	3.25
6	0.43988269794721413	1.7999999999999998
7	0.32991202346041054	1.575
8	0.21994134897360706	1.2
9	0.4032258064516129	2.475
>10	1.0997067448680353	11.55
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCGAATACACCAGCTACACCTAACATGTGAAATGGGTGCATAAGAATGTTGTGCTCAGCCTGGAATACAATCAT	35	0.8750000000000001	No Hit
CAAGGAACCATGCATAGCACTAAAAAGGGAGCCGCCGAATACACCAGCTACACCTAACATGTGAAATGGGTGCAT	29	0.7250000000000001	No Hit
GGCCAAGCCGCTAAGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAAAATCAATCGGCCAAAA	24	0.6	No Hit
GTTTTTTACTAAACTAGATCTAGACTAACACTAACGAATTATCCATTTGTAGATGGAGCCTCAACAGCAGCTAGG	23	0.575	No Hit
CGGAAACTAAGTTCCCACTCACGACCCATATAACAAGCTACACCAAGTAAAAAGTGTAGAACAATTAGTTCATAA	23	0.575	No Hit
CAATAAGGTAGGGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAGTGCTAGTTATCCAGTTACA	23	0.575	No Hit
GCGGTCAATAAGGTAGGGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAGTGCTAGTTATCCAG	18	0.44999999999999996	No Hit
GAAAAACTTCCCTGACCAATTGGATAGATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCAACA	18	0.44999999999999996	No Hit
CCGGCCAAGCCGCTAAGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAAAATCAATCGGCCAA	16	0.4	No Hit
GTTGAAACTAGCATATTGGAAAATCAATCGGCCAAAATAACCGTGAGCAGCTACAATGTTGTAAGTTTCTTCTTC	16	0.4	No Hit
GGTCAATAAGGTAGGGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAGTGCTAGTTATCCAGTT	16	0.4	No Hit
GCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAACACCAAACCATCCA	15	0.375	No Hit
CATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTAGGAATAATGGCACCGG	15	0.375	No Hit
GTCAATAAGGTAGGGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAGTGCTAGTTATCCAGTTA	14	0.35000000000000003	No Hit
CTACAATGTTGTAAGTTTCTTCTTCTTGCCCGAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTTTCCC	14	0.35000000000000003	No Hit
CAATGTTGTAAGTTTCTTCTTCTTGCCCGAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTTTCCCTGA	12	0.3	No Hit
CGAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTTTCCCTGATCAAACTAGAAGTTACCAAGGAACCAT	12	0.3	No Hit
GTCCAATCGGAGCGGCTTATTGGGTCATAGTGGGCTCGGAAGGCAATGGCCCGTAATCCTTCCACCGTCAAGAGC	12	0.3	No Hit
CTCAACAGCAGCTAGGTCTAGAGGGAAGTTGTGAGCATTACGTTCATGCATAACTTCCATACCAAGGTTAGCACG	12	0.3	No Hit
CAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAACACCAAACCATCCAA	12	0.3	No Hit
AGCAGCTACAATGTTGTAAGTTTCTTCTTCTTGCCCGAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGT	12	0.3	No Hit
GGAGCCTCAACAGCAGCTAGGTCTAGAGGGAAGTTGTGAGCATTACGTTCATGCATAACTTCCATACCAAGGTTA	11	0.27499999999999997	No Hit
CTAGAGGCATACCATCAGAAAAACTTCCCTGACCAATTGGATAGATCAAGAAAACAGCAGTCGCAGCTGCAACAG	10	0.25	No Hit
CTCGTTTTTTACTAAACTAGATCTAGACTAACACTAACGAATTATCCATTTGTAGATGGAGCCTCAACAGCAGCT	10	0.25	No Hit
CGGCCAAGCCGCTAAGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAAAATCAATCGGCCAAA	10	0.25	No Hit
TAAGAATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACCATCAG	10	0.25	No Hit
CAAGCCGCTAAGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAAAATCAATCGGCCAAAATAA	10	0.25	No Hit
GGAGGAGCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAACACCAAAC	10	0.25	No Hit
CCTCAACAGCAGCTAGGTCTAGAGGGAAGTTGTGAGCATTACGTTCATGCATAACTTCCATACCAAGGTTAGCAC	10	0.25	No Hit
CCACTCACGACCCATATAACAAGCTACACCAAGTAAAAAGTGTAGAACAATTAGTTCATAAGGACCGCCGTTGTA	10	0.25	No Hit
GCCTCAACAGCAGCTAGGTCTAGAGGGAAGTTGTGAGCATTACGTTCATGCATAACTTCCATACCAAGGTTAGCA	9	0.22499999999999998	No Hit
GGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAGTGCTAGTTATCCAGTTACAGAAGCGACCCC	9	0.22499999999999998	No Hit
GTGCATAAGAATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACC	9	0.22499999999999998	No Hit
AAGAATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACCATCAGA	9	0.22499999999999998	No Hit
CAGAAGCGACCCCATAGGCTTTCGCTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAAATCCTTGGTTTATTTA	9	0.22499999999999998	No Hit
GAAAGTTTTAGTATAAGCAGGAGGGATTCGCAGATCCTCTAGACGTAGAGCAGCCAGGGCTTTGAACCCAAATAC	9	0.22499999999999998	No Hit
CGCATACCCAGACGGAAACTAAGTTCCCACTCACGACCCATATAACAAGCTACACCAAGTAAAAAGTGTAGAACA	9	0.22499999999999998	No Hit
AGAATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACCATCAGAA	9	0.22499999999999998	No Hit
GGAAAATCAATCGGCCAAAATAACCGTGAGCAGCTACAATGTTGTAAGTTTCTTCTTCTTGCCCGAATCTGTAAC	9	0.22499999999999998	No Hit
TGGAGGAGCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAACACCAAA	9	0.22499999999999998	No Hit
CACGAATACCATCAATATCTACTGGAGGAGCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGG	9	0.22499999999999998	No Hit
CAGTTACAGAAGCGACCCCATAGGCTTTCGCTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAAATCCTTGGTT	8	0.2	No Hit
CTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCCTGACCAAT	8	0.2	No Hit
ATAATATCAGCCCAAGTATTAATAACACGTCCTTGACTATCAACTACTGATTGGTTGAAATTGAAACCATTTAGG	8	0.2	No Hit
TAGCCATTCATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTAGGAATAAT	8	0.2	No Hit
CATTCATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTAGGAATAATGGCA	8	0.2	No Hit
AGAAGCGACCCCATAGGCTTTCGCTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAAATCCTTGGTTTATTTAA	8	0.2	No Hit
GCCAAGCCGCTAAGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAAAATCAATCGGCCAAAAT	7	0.17500000000000002	No Hit
ATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTAGGAATAATGGCACCGGA	7	0.17500000000000002	No Hit
CTTGACTATCAACTACTGATTGGTTGAAATTGAAACCATTTAGGTTGAAAGCCATAGTACTAATACCTAAAGCAG	7	0.17500000000000002	No Hit
GGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCCTGACCAATTG	7	0.17500000000000002	No Hit
GGGAAGTTGTGAGCATTACGTTCATGCATAACTTCCATACCAAGGTTAGCACGGTTAATAATATCAGCCCAAGTA	7	0.17500000000000002	No Hit
GGAGCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAACACCAAACCAT	7	0.17500000000000002	No Hit
AAAAAGGGAGCCGCCGAATACACCAGCTACACCTAACATGTGAAATGGGTGCATAAGAATGTTGTGCTCAGCCTG	7	0.17500000000000002	No Hit
AAAAACTTCCCTGACCAATTGGATAGATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCAACAG	7	0.17500000000000002	No Hit
ACAGAAGCGACCCCATAGGCTTTCGCTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAAATCCTTGGTTTATTT	7	0.17500000000000002	No Hit
CTGGAGGAGCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAACACCAA	6	0.15	No Hit
GGGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAGTGCTAGTTATCCAGTTACAGAAGCGACCC	6	0.15	No Hit
GGCATACCATCAGAAAAACTTCCCTGACCAATTGGATAGATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCT	6	0.15	No Hit
ATTACGTTCATGCATAACTTCCATACCAAGGTTAGCACGGTTAATAATATCAGCCCAAGTATTAATAACACGTCC	6	0.15	No Hit
GTAAGTTTCTTCTTCTTGCCCGAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTTTCCCTGATCAAACT	6	0.15	No Hit
CTCAGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCCTG	6	0.15	No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAAATCAATCGGCCAAAATAACCGTGAGCAGCTACAATGTTGTAAGTT	6	0.15	No Hit
GTGAAATGGGTGCATAAGAATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAG	6	0.15	No Hit
GCCGTTGTATAGCCATTCATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGT	6	0.15	No Hit
AGCAGTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGACGCATACCCAGACGGAAACTAAGTTC	6	0.15	No Hit
GTTGTATAGCCATTCATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTAGG	6	0.15	No Hit
TAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAG	6	0.15	No Hit
CCGTTGTATAGCCATTCATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTA	5	0.125	No Hit
GCCATTCATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTAGGAATAATGG	5	0.125	No Hit
CAGCTACAATGTTGTAAGTTTCTTCTTCTTGCCCGAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTTT	5	0.125	No Hit
GGTGCATAAGAATGTTGTGCTCAGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATAC	5	0.125	No Hit
TAAAAATGCAATCCAATAGCTGCAGAAGTAGGAATAATGGCACCGGAAATAATATTGTTTCCGTAAAGAAGAGAT	5	0.125	No Hit
AGGAGCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAACACCAAACCA	5	0.125	No Hit
ATTCATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTAGGAATAATGGCAC	5	0.125	No Hit
CGGTCAATAAGGTAGGGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAGTGCTAGTTATCCAGT	5	0.125	No Hit
CGGTTTTCAGTGCTAGTTATCCAGTTACAGAAGCGACCCCATAGGCTTTCGCTTTCGCGTCTCTCTAAAATTGCA	5	0.125	No Hit
GAGCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAACACCAAACCATC	5	0.125	No Hit
CAAAGATCTCGGTCAAAGCAGGCATATGCCAAACGTGAATACCCCCTGAAGCCACAGGCAGAACACCAGGTAGTG	5	0.125	No Hit
ATAGGCTTTCGCTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAAATCCTTGGTTTATTTAATAATCAGGGACT	5	0.125	No Hit
CTTATATTGCTCGTTTTTTACTAAACTAGATCTAGACTAACACTAACGAATTATCCATTTGTAGATGGAGCCTCA	5	0.125	No Hit
AATAAGGTAGGGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAGTGCTAGTTATCCAGTTACAG	5	0.125	No Hit
CGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGACGCATACCCAGACGGAAACTAAGTTCCCACTC	5	0.125	No Hit
GTTACAGAAGCGACCCCATAGGCTTTCGCTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAAATCCTTGGTTTA	5	0.125	No Hit
CTAATACCTAAAGCAGTAAACCAAATACCTACTACCGGCCAAGCCGCTAAGAAGAAATGTAAAGAACGAGAATTG	5	0.125	No Hit
TATAGCCATTCATCAACGGATGCAGCTTCCCAGATTGGGTAAAAATGCAATCCAATAGCTGCAGAAGTAGGAATA	5	0.125	No Hit
GCACTAAAAAGGGAGCCGCCGAATACACCAGCTACACCTAACATGTGAAATGGGTGCATAAGAATGTTGTGCTCA	5	0.125	No Hit
GGTAGGGATCATCAAAACACCAAACCATCCAATGTAAAGACGGTTTTCAGTGCTAGTTATCCAGTTACAGAAGCG	5	0.125	No Hit
CCAGTTACAGAAGCGACCCCATAGGCTTTCGCTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAAATCCTTGGT	5	0.125	No Hit
CGAATACCATCAATATCTACTGGAGGAGCAGCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTA	5	0.125	No Hit
CTTCTTCTTGCCCGAATCTGTAACCTTCATTAGCAGATTCATTTTCTGTGGTTTCCCTGATCAAACTAGAAGTTA	5	0.125	No Hit
ATCAGCCCAAGTATTAATAACACGTCCTTGACTATCAACTACTGATTGGTTGAAATTGAAACCATTTAGGTTGAA	5	0.125	No Hit
GCAATGAATGCGATAATAAAAACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAACACCAAACCATCCAATG	5	0.125	No Hit
CAGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCCTGAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.025	0.0	0.0	0.0	0.0
43	0.025	0.0	0.0	0.0	0.0
44	0.025	0.0	0.0	0.0	0.0
45	0.025	0.0	0.0	0.0	0.0
46	0.025	0.0	0.0	0.0	0.0
47	0.025	0.0	0.0	0.0	0.0
48	0.025	0.0	0.0	0.0	0.0
49	0.025	0.0	0.0	0.0	0.0
50	0.025	0.0	0.0	0.0	0.0
51	0.075	0.0	0.0	0.0	0.0
52	0.075	0.0	0.0	0.0	0.0
53	0.075	0.0	0.0	0.0	0.0
54	0.075	0.0	0.0	0.0	0.0
55	0.075	0.0	0.0	0.0	0.0
56	0.1	0.0	0.0	0.0	0.0
57	0.175	0.0	0.0	0.0	0.0
58	0.175	0.0	0.0	0.0	0.0
59	0.175	0.0	0.0	0.0	0.0
60	0.225	0.0	0.0	0.0	0.0
61	0.25	0.0	0.0	0.0	0.0
62	0.275	0.0	0.0	0.0	0.0
63	0.3	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR30509053 read2 length is 75 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR30509053_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	75
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.94925	32.0	32.0	32.0	27.0	32.0
2	31.4435	32.0	32.0	32.0	32.0	32.0
3	34.8425	37.0	37.0	37.0	32.0	37.0
4	35.62975	37.0	37.0	37.0	32.0	37.0
5	35.82475	37.0	37.0	37.0	32.0	37.0
6	39.73475	42.0	37.0	42.0	37.0	42.0
7	39.918	42.0	42.0	42.0	37.0	42.0
8	39.96375	42.0	42.0	42.0	37.0	42.0
9	39.61875	42.0	42.0	42.0	32.0	42.0
10	39.38525	42.0	42.0	42.0	32.0	42.0
11	40.24475	42.0	42.0	42.0	37.0	42.0
12	40.12275	42.0	42.0	42.0	37.0	42.0
13	40.15825	42.0	42.0	42.0	37.0	42.0
14	40.13425	42.0	42.0	42.0	37.0	42.0
15	39.94475	42.0	42.0	42.0	37.0	42.0
16	39.88425	42.0	42.0	42.0	37.0	42.0
17	39.87725	42.0	42.0	42.0	37.0	42.0
18	39.799	42.0	42.0	42.0	37.0	42.0
19	39.58775	42.0	42.0	42.0	32.0	42.0
20	39.845	42.0	42.0	42.0	37.0	42.0
21	39.6855	42.0	42.0	42.0	32.0	42.0
22	39.54925	42.0	42.0	42.0	32.0	42.0
23	39.52875	42.0	42.0	42.0	32.0	42.0
24	39.34825	42.0	42.0	42.0	32.0	42.0
25	39.677	42.0	42.0	42.0	32.0	42.0
26	39.60425	42.0	42.0	42.0	32.0	42.0
27	39.39375	42.0	42.0	42.0	32.0	42.0
28	39.5435	42.0	42.0	42.0	32.0	42.0
29	39.4115	42.0	42.0	42.0	32.0	42.0
30	39.5565	42.0	42.0	42.0	32.0	42.0
31	39.526	42.0	42.0	42.0	32.0	42.0
32	39.27425	42.0	42.0	42.0	32.0	42.0
33	39.54	42.0	42.0	42.0	32.0	42.0
34	39.04825	42.0	37.0	42.0	32.0	42.0
35	39.1855	42.0	42.0	42.0	32.0	42.0
36	39.1515	42.0	37.0	42.0	32.0	42.0
37	39.225	42.0	42.0	42.0	32.0	42.0
38	39.046	42.0	37.0	42.0	32.0	42.0
39	39.0845	42.0	37.0	42.0	32.0	42.0
40	39.06375	42.0	37.0	42.0	32.0	42.0
41	38.61225	42.0	37.0	42.0	32.0	42.0
42	38.38275	42.0	37.0	42.0	32.0	42.0
43	39.08575	42.0	37.0	42.0	32.0	42.0
44	38.86075	42.0	37.0	42.0	32.0	42.0
45	39.1665	42.0	37.0	42.0	32.0	42.0
46	38.8035	42.0	37.0	42.0	32.0	42.0
47	38.48775	42.0	37.0	42.0	32.0	42.0
48	39.00625	42.0	37.0	42.0	32.0	42.0
49	39.19125	42.0	37.0	42.0	32.0	42.0
50	38.5355	42.0	37.0	42.0	32.0	42.0
51	38.438	42.0	37.0	42.0	32.0	42.0
52	38.92325	42.0	37.0	42.0	32.0	42.0
53	38.01375	42.0	37.0	42.0	27.0	42.0
54	38.90625	42.0	37.0	42.0	32.0	42.0
55	39.1325	42.0	37.0	42.0	32.0	42.0
56	39.094	42.0	37.0	42.0	32.0	42.0
57	38.921	42.0	37.0	42.0	32.0	42.0
58	39.202	42.0	42.0	42.0	32.0	42.0
59	39.07225	42.0	37.0	42.0	32.0	42.0
60	39.001	42.0	37.0	42.0	32.0	42.0
61	37.685	42.0	37.0	42.0	27.0	42.0
62	37.86575	42.0	37.0	42.0	27.0	42.0
63	37.97	42.0	37.0	42.0	27.0	42.0
64	38.07925	42.0	37.0	42.0	32.0	42.0
65	38.3365	42.0	37.0	42.0	32.0	42.0
66	38.547	42.0	37.0	42.0	32.0	42.0
67	38.17225	42.0	37.0	42.0	32.0	42.0
68	38.547	42.0	37.0	42.0	32.0	42.0
69	38.73175	42.0	37.0	42.0	32.0	42.0
70	38.22475	42.0	37.0	42.0	32.0	42.0
71	38.1295	42.0	37.0	42.0	27.0	42.0
72	38.4835	42.0	37.0	42.0	32.0	42.0
73	37.7465	42.0	37.0	42.0	27.0	42.0
74	38.11	42.0	37.0	42.0	32.0	42.0
75	37.86675	42.0	37.0	42.0	27.0	42.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
1101	52	0.0
1101	53	0.0
1101	54	0.0
1101	55	0.0
1101	56	0.0
1101	57	0.0
1101	58	0.0
1101	59	0.0
1101	60	0.0
1101	61	0.0
1101	62	0.0
1101	63	0.0
1101	64	0.0
1101	65	0.0
1101	66	0.0
1101	67	0.0
1101	68	0.0
1101	69	0.0
1101	70	0.0
1101	71	0.0
1101	72	0.0
1101	73	0.0
1101	74	0.0
1101	75	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	0.0
18	6.0
19	3.0
20	6.0
21	6.0
22	7.0
23	10.0
24	17.0
25	19.0
26	22.0
27	30.0
28	30.0
29	38.0
30	53.0
31	45.0
32	62.0
33	84.0
34	91.0
35	110.0
36	150.0
37	195.0
38	280.0
39	487.0
40	1045.0
41	1202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.725	16.5	19.55	29.225
2	27.35	21.75	34.949999999999996	15.950000000000001
3	22.0	26.3	34.150000000000006	17.549999999999997
4	26.85	33.4	23.35	16.400000000000002
5	24.099999999999998	34.775	24.275	16.85
6	18.175	34.75	28.7	18.375
7	18.3	15.775	45.074999999999996	20.849999999999998
8	20.200000000000003	21.275	30.15	28.375
9	22.075	22.5	33.425	22.0
10	22.125	34.1	27.35	16.425
11	27.224999999999998	23.125	24.875	24.775
12	26.400000000000002	19.175	29.099999999999998	25.324999999999996
13	23.325000000000003	24.125	31.724999999999998	20.825
14	22.5	24.65	33.45	19.400000000000002
15	21.375	26.525	32.6	19.5
16	23.05	24.925	31.95	20.075000000000003
17	27.224999999999998	24.675	27.825	20.275000000000002
18	21.975	27.525	31.55	18.95
19	25.525	25.825	29.2	19.45
20	24.925	26.1	29.549999999999997	19.425
21	23.599999999999998	25.55	32.125	18.725
22	21.8	25.674999999999997	31.85	20.674999999999997
23	20.724999999999998	26.650000000000002	33.025	19.6
24	23.1	25.1	31.574999999999996	20.225
25	23.225	23.724999999999998	30.375000000000004	22.675
26	21.675	26.174999999999997	32.175	19.975
27	20.674999999999997	28.9	31.525	18.9
28	20.875	27.775	30.25	21.099999999999998
29	20.474999999999998	27.825	31.424999999999997	20.275000000000002
30	21.325	27.35	31.125000000000004	20.200000000000003
31	22.95	27.6	30.425	19.025
32	24.15	24.0	32.525	19.325
33	21.65	26.05	32.45	19.85
34	20.775	28.9	30.55	19.775000000000002
35	22.55	29.375	28.449999999999996	19.625
36	20.65	26.85	31.025000000000002	21.475
37	22.45	26.400000000000002	28.349999999999998	22.8
38	21.625	29.475	30.325000000000003	18.575
39	20.65	27.500000000000004	32.45	19.400000000000002
40	21.925	27.6	30.825000000000003	19.650000000000002
41	22.85	25.974999999999998	29.175	22.0
42	20.825	28.799999999999997	30.875000000000004	19.5
43	22.1	25.900000000000002	32.05	19.950000000000003
44	22.975	27.1	31.275	18.65
45	23.549999999999997	26.400000000000002	31.45	18.6
46	21.65	27.150000000000002	30.475	20.724999999999998
47	21.224999999999998	24.349999999999998	33.275	21.15
48	22.225	25.224999999999998	32.15	20.4
49	23.3	23.849999999999998	33.0	19.85
50	22.1	25.674999999999997	32.775	19.45
51	21.25	25.4	32.375	20.974999999999998
52	21.6	25.35	29.625	23.425
53	21.3	26.85	28.999999999999996	22.85
54	21.5	25.174999999999997	31.35	21.975
55	22.225	26.85	30.55	20.375
56	23.775	25.324999999999996	32.025	18.875
57	21.875	26.025	31.225	20.875
58	21.95	26.450000000000003	30.65	20.95
59	20.849999999999998	25.45	32.35	21.349999999999998
60	23.125	23.65	31.324999999999996	21.9
61	21.5	25.1	30.599999999999998	22.8
62	22.325	25.95	31.0	20.724999999999998
63	20.775	26.424999999999997	32.074999999999996	20.724999999999998
64	20.225	29.15	32.425	18.2
65	21.55	24.725	33.650000000000006	20.075000000000003
66	21.85	28.275	29.75	20.125
67	20.5	24.875	33.975	20.65
68	21.8	26.8	30.375000000000004	21.025
69	23.549999999999997	26.55	29.599999999999998	20.3
70	22.525000000000002	26.950000000000003	31.025000000000002	19.5
71	23.175	26.950000000000003	30.349999999999998	19.525000000000002
72	22.85	26.674999999999997	30.625000000000004	19.85
73	24.425	26.35	30.25	18.975
74	23.925	26.025	29.049999999999997	21.0
75	20.875	26.450000000000003	32.725	19.950000000000003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	0.5
18	3.0
19	3.5
20	1.0
21	4.0
22	7.5
23	9.5
24	11.0
25	12.5
26	15.5
27	19.0
28	21.0
29	21.0
30	26.0
31	35.5
32	40.0
33	63.0
34	90.0
35	143.5
36	193.0
37	252.5
38	337.0
39	344.5
40	327.0
41	336.5
42	308.5
43	293.0
44	315.0
45	321.5
46	326.0
47	242.5
48	161.0
49	152.0
50	134.0
51	117.0
52	109.0
53	88.5
54	70.0
55	59.0
56	46.0
57	42.5
58	34.0
59	28.0
60	27.0
61	21.5
62	15.0
63	9.5
64	5.0
65	5.0
66	3.5
67	1.5
68	1.0
69	0.5
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.38812785388129	60.775
2	7.6923076923076925	10.95
3	2.212855637513172	4.725
4	1.36986301369863	3.9
5	0.8429926238145415	3.0
6	0.7376185458377239	3.15
7	0.5619950825430278	2.8000000000000003
8	0.2809975412715139	1.6
9	0.17562346329469616	1.125
>10	0.7376185458377239	7.9750000000000005
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTGAACCTGTTTCTGGATCTCTTCTTTACGGAAACAATATTATTTCCGGTGCCATTATTCCTACTTCTGCAGCT	36	0.8999999999999999	No Hit
AGAATTTGTGCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAGAC	33	0.8250000000000001	No Hit
GAGAATTTGTGCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAGA	28	0.7000000000000001	No Hit
GTTTGATCAGGGAAACCACAGAAAATGAATCTGCTAATGAAGGTTACAGATTCGGGCAAGAAGAAGAAACTTACA	21	0.525	No Hit
GCGAAAGCGAAAGCCTATGGGGTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGTCTTTACATTGGATGGT	19	0.475	No Hit
GAATTTGTGCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAGACG	14	0.35000000000000003	No Hit
GCTGCGACTGCTGTTTTCTTGATCTATCCAATTGGTCAGGGAAGTTTTTCTGATGGTATGCCTCTAGGAATCTCT	13	0.325	No Hit
CATGACTGCAATTTTAGAGAGACGCGAAAGCGAAAGCCTATGGGGTCGCTTCTGTAACTGGATAACTAGCACTGA	13	0.325	No Hit
TTTAGAGAGACGCGAAAGCGAAAGCCTATGGGGTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGTCTTTA	13	0.325	No Hit
TATTAACCGTGCTAACCTTGGTATGGAAGTTATGCATGAACGTAATGCTCACAACTTCCCTCTAGACCTAGCTGC	12	0.3	No Hit
GGTACTTTCAACTTTATGATTGTATTCCAGGCTGAGCACAACATTCTTATGCACCCATTTCACATGTTAGGTGTA	12	0.3	No Hit
TCTACTTAGAGAATTTGTGCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTT	11	0.27499999999999997	No Hit
TGCTAATGAAGGTTACAGATTCGGGCAAGAAGAAGAAACTTACAACATTGTAGCTGCTCACGGTTATTTTGGCCG	11	0.27499999999999997	No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTTTATGATTGTATTCCAGGCTGAGCACAACA	11	0.27499999999999997	No Hit
GTTAGGTGTAGCTGGTGTATTCGGCGGCTCCCTTTTTAGTGCTATGCATGGTTCCTTGGTAACTTCTAGTTTGAT	11	0.27499999999999997	No Hit
TGGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACT	11	0.27499999999999997	No Hit
TGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTTTATGATTGTATTCCAGGCTGAGCACAACATTCTTAT	10	0.25	No Hit
GCGAAAGCCTATGGGGTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGTCTTTACATTGGATGGTTTGGTG	10	0.25	No Hit
CTTGATTTTACCAAAGATGATGAGAATGTGAACTCCCAACCATTTATGCGTTGGAGAGACCGTTTCTTATTTTGT	10	0.25	No Hit
GCTGCATCCGTTGATGAATGGCTATACAACGGCGGTCCTTATGAACTAATTGTTCTACACTTTTTACTTGGTGTA	10	0.25	No Hit
GAAACTTACAACATTGTAGCTGCTCACGGTTATTTTGGCCGATTGATTTTCCAATATGCTAGTTTCAACAATTCT	10	0.25	No Hit
CAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATCTATCCAATTGGTCAGGGAAGTTTTTCTGATGGTATGC	9	0.22499999999999998	No Hit
CTGGATAACTAGCACTGAAAACCGTCTTTACATTGGATGGTTTGGTGTTTTGATGATCCCTACCTTATTGACCGC	9	0.22499999999999998	No Hit
CTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAGACGCGAAAGCGAAA	9	0.22499999999999998	No Hit
AAGGATTTTACCATGACTGCAATTTTAGAGAGACGCGAAAGCGAAAGCCTATGGGGTCGCTTCTGTAACTGGATA	9	0.22499999999999998	No Hit
TTTGGTTTACTGCTTTAGGTATTAGTACTATGGCTTTCAACCTAAATGGTTTCAATTTCAACCAATCAGTAGTTG	9	0.22499999999999998	No Hit
AGAGAATTTGTGCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAG	8	0.2	No Hit
GTAACTTCTAGTTTGATCAGGGAAACCACAGAAAATGAATCTGCTAATGAAGGTTACAGATTCGGGCAAGAAGAA	8	0.2	No Hit
TTTCAACCAATCAGTAGTTGATAGTCAAGGACGTGTTATTAATACTTGGGCTGATATTATTAACCGTGCTAACCT	8	0.2	No Hit
GTGCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAGACGCGAAAG	8	0.2	No Hit
CAACATTCTTATGCACCCATTTCACATGTTAGGTGTAGCTGGTGTATTCGGCGGCTCCCTTTTTAGTGCTATGCA	8	0.2	No Hit
CAATTATCTACTTAGAGAATTTGTGCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTG	8	0.2	No Hit
CACATGTTAGGTGTAGCTGGTGTATTCGGCGGCTCCCTTTTTAGTGCTATGCATGGTTCCTTGGTAACTTCTAGT	8	0.2	No Hit
GGCGGCTCCCTTTTTAGTGCTATGCATGGTTCCTTGGTAACTTCTAGTTTGATCAGGGAAACCACAGAAAATGAA	8	0.2	No Hit
CGGCGGTCCTTATGAACTAATTGTTCTACACTTTTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGAACT	7	0.17500000000000002	No Hit
CTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATATTCAG	7	0.17500000000000002	No Hit
CAAGGATTTTACCATGACTGCAATTTTAGAGAGACGCGAAAGCGAAAGCCTATGGGGTCGCTTCTGTAACTGGAT	7	0.17500000000000002	No Hit
TTTCAACCTAAATGGTTTCAATTTCAACCAATCAGTAGTTGATAGTCAAGGACGTGTTATTAATACTTGGGCTGA	7	0.17500000000000002	No Hit
CTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTTTATGATTGTATTCCAGGCTGAGCACAACATTCTTA	7	0.17500000000000002	No Hit
GGAAACCACAGAAAATGAATCTGCTAATGAAGGTTACAGATTCGGGCAAGAAGAAGAAACTTACAACATTGTAGC	7	0.17500000000000002	No Hit
GTTTACTGCTTTAGGTATTAGTACTATGGCTTTCAACCTAAATGGTTTCAATTTCAACCAATCAGTAGTTGATAG	7	0.17500000000000002	No Hit
TTTACATTGGATGGTTTGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTTTTTATTATCGCATTCA	7	0.17500000000000002	No Hit
GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATCTATCCAATTGGTCAGGGAAGTTTTTCT	7	0.17500000000000002	No Hit
CTGGTACTTTCAACTTTATGATTGTATTCCAGGCTGAGCACAACATTCTTATGCACCCATTTCACATGTTAGGTG	7	0.17500000000000002	No Hit
CAACATTGTAGCTGCTCACGGTTATTTTGGCCGATTGATTTTCCAATATGCTAGTTTCAACAATTCTCGTTCTTT	7	0.17500000000000002	No Hit
AGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATCTATCCAATTGGTCAGGGAAGTTTTTCTGATGGTATGCC	7	0.17500000000000002	No Hit
GGTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGTCTTTACATTGGATGGTTTGGTGTTTTGATGATCCCT	7	0.17500000000000002	No Hit
CCTACTTCTGCAGCTATTGGATTGCATTTTTACCCAATCTGGGAAGCTGCATCCGTTGATGAATGGCTATACAAC	7	0.17500000000000002	No Hit
CCGGTAGTAGGTATTTGGTTTACTGCTTTAGGTATTAGTACTATGGCTTTCAACCTAAATGGTTTCAATTTCAAC	7	0.17500000000000002	No Hit
AATTTGTGCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAGACGC	7	0.17500000000000002	No Hit
GCAATTTTAGAGAGACGCGAAAGCGAAAGCCTATGGGGTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGT	6	0.15	No Hit
GTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCT	6	0.15	No Hit
TGGCTTTCAACCTAAATGGTTTCAATTTCAACCAATCAGTAGTTGATAGTCAAGGACGTGTTATTAATACTTGGG	6	0.15	No Hit
GTTTTCTTGATCTATCCAATTGGTCAGGGAAGTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAAC	6	0.15	No Hit
GCTATACAACGGCGGTCCTTATGAACTAATTGTTCTACACTTTTTACTTGGTGTAGCTTGTTATATGGGTCGTGA	6	0.15	No Hit
CTGCAATTTTAGAGAGACGCGAAAGCGAAAGCCTATGGGGTCGCTTCTGTAACTGGATAACTAGCACTGAAAACC	6	0.15	No Hit
GTTCTACACTTTTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTCTGGGTATGCGT	6	0.15	No Hit
CTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATCTATCCAATTGGTCAGG	6	0.15	No Hit
GTGAACCTGTTTCTGGATCTCTTCTTTACGGAAACAATATTATTTCCGGTGCCATTATTCCTACTTCTGCAGCTA	6	0.15	No Hit
AACATTCTTATGCACCCATTTCACATGTTAGGTGTAGCTGGTGTATTCGGCGGCTCCCTTTTTAGTGCTATGCAT	6	0.15	No Hit
CGAAAGCCTATGGGGTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGTCTTTACATTGGATGGTTTGGTGT	6	0.15	No Hit
GGAAGTTATGCATGAACGTAATGCTCACAACTTCCCTCTAGACCTAGCTGCTGTTGAGGCTCCATCTACAAATGG	6	0.15	No Hit
TGGAAGTTATGCATGAACGTAATGCTCACAACTTCCCTCTAGACCTAGCTGCTGTTGAGGCTCCATCTACAAATG	6	0.15	No Hit
GGCAAGAAGAAGAAACTTACAACATTGTAGCTGCTCACGGTTATTTTGGCCGATTGATTTTCCAATATGCTAGTT	6	0.15	No Hit
GGAAGCTGCATCCGTTGATGAATGGCTATACAACGGCGGTCCTTATGAACTAATTGTTCTACACTTTTTACTTGG	6	0.15	No Hit
GGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGC	6	0.15	No Hit
GTACTTTCAACTTTATGATTGTATTCCAGGCTGAGCACAACATTCTTATGCACCCATTTCACATGTTAGGTGTAG	6	0.15	No Hit
GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATATTCA	6	0.15	No Hit
TCTGGATCTCTTCTTTACGGAAACAATATTATTTCCGGTGCCATTATTCCTACTTCTGCAGCTATTGGATTGCAT	6	0.15	No Hit
GTCAAGGACGTGTTATTAATACTTGGGCTGATATTATTAACCGTGCTAACCTTGGTATGGAAGTTATGCATGAAC	6	0.15	No Hit
CTTAGAGAATTTGTGCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTTTAGA	6	0.15	No Hit
AATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAGACGCGAAAGCGAAAGCCTATGGGGTCGCTTCTGTA	5	0.125	No Hit
ACAACATTGTAGCTGCTCACGGTTATTTTGGCCGATTGATTTTCCAATATGCTAGTTTCAACAATTCTCGTTCTT	5	0.125	No Hit
CTGGGAAGCTGCATCCGTTGATGAATGGCTATACAACGGCGGTCCTTATGAACTAATTGTTCTACACTTTTTACT	5	0.125	No Hit
TCCTACTTCTGCAGCTATTGGATTGCATTTTTACCCAATCTGGGAAGCTGCATCCGTTGATGAATGGCTATACAA	5	0.125	No Hit
CAACAATTCTCGTTCTTTACATTTCTTCTTAGCGGCTTGGCCGGTAGTAGGTATTTGGTTTACTGCTTTAGGTAT	5	0.125	No Hit
ATGGAAGTTATGCATGAACGTAATGCTCACAACTTCCCTCTAGACCTAGCTGCTGTTGAGGCTCCATCTACAAAT	5	0.125	No Hit
GGCCGATTGATTTTCCAATATGCTAGTTTCAACAATTCTCGTTCTTTACATTTCTTCTTAGCGGCTTGGCCGGTA	5	0.125	No Hit
CCTGTTTCTGGATCTCTTCTTTACGGAAACAATATTATTTCCGGTGCCATTATTCCTACTTCTGCAGCTATTGGA	5	0.125	No Hit
CCAGGCTGAGCACAACATTCTTATGCACCCATTTCACATGTTAGGTGTAGCTGGTGTATTCGGCGGCTCCCTTTT	5	0.125	No Hit
AAATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAGACGCGAAAGCGAAAGCCTATGGGGTCGCTTCTGT	5	0.125	No Hit
TGCGCTTGGGAGTCCCTGATTATTAAATAAACCAAGGATTTTACCATGACTGCAATTTTAGAGAGACGCGAAAGC	5	0.125	No Hit
ATCGAAGCCGCGGTATCTTTTTCACTCAAGATTGGGTCTCACTACCTGGTGTTCTGCCTGTGGCTTCAGGGGGTA	5	0.125	No Hit
CTGCATCCGTTGATGAATGGCTATACAACGGCGGTCCTTATGAACTAATTGTTCTACACTTTTTACTTGGTGTAG	5	0.125	No Hit
GGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTG	5	0.125	No Hit
GTCGCTTCTGTAACTGGATAACTAGCACTGAAAACCGTCTTTACATTGGATGGTTTGGTGTTTTGATGATCCCTA	5	0.125	No Hit
ATTCGGGCAAGAAGAAGAAACTTACAACATTGTAGCTGCTCACGGTTATTTTGGCCGATTGATTTTCCAATATGC	5	0.125	No Hit
TGAATGGCTATACAACGGCGGTCCTTATGAACTAATTGTTCTACACTTTTTACTTGGTGTAGCTTGTTATATGGG	5	0.125	No Hit
CAATCTGGGAAGCTGCATCCGTTGATGAATGGCTATACAACGGCGGTCCTTATGAACTAATTGTTCTACACTTTT	5	0.125	No Hit
CTGGATCTCTTCTTTACGGAAACAATATTATTTCCGGTGCCATTATTCCTACTTCTGCAGCTATTGGATTGCATT	5	0.125	No Hit
CAAGGACGTGTTATTAATACTTGGGCTGATATTATTAACCGTGCTAACCTTGGTATGGAAGTTATGCATGAACGT	5	0.125	No Hit
TCTAGTTTGATCAGGGAAACCACAGAAAATGAATCTGCTAATGAAGGTTACAGATTCGGGCAAGAAGAAGAAACT	5	0.125	No Hit
CTCTGGTACTTTCAACTTTATGATTGTATTCCAGGCTGAGCACAACATTCTTATGCACCCATTTCACATGTTAGG	5	0.125	No Hit
GTTGATAGTCAAGGACGTGTTATTAATACTTGGGCTGATATTATTAACCGTGCTAACCTTGGTATGGAAGTTATG	5	0.125	No Hit
GCTGCTCCTCCAGTAGATATTGATGGTATTCGTGAACCTGTTTCTGGATCTCTTCTTTACGGAAACAATATTATT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.05	0.0	0.0	0.0	0.0
52	0.05	0.0	0.0	0.0	0.0
53	0.05	0.0	0.0	0.0	0.0
54	0.05	0.0	0.0	0.0	0.0
55	0.05	0.0	0.0	0.0	0.0
56	0.075	0.0	0.0	0.0	0.0
57	0.15	0.0	0.0	0.0	0.0
58	0.15	0.0	0.0	0.0	0.0
59	0.15	0.0	0.0	0.0	0.0
60	0.2	0.0	0.0	0.0	0.0
61	0.225	0.0	0.0	0.0	0.0
62	0.275	0.0	0.0	0.0	0.0
63	0.3	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 45426391 spots for SRR30509053.sra
Written 45426391 spots for SRR30509053.sra
Read 45426391 spots for SRR30509053.sra
Written 45426391 spots for SRR30509053.sra
Read 45426392 spots for SRR30509053.sra
Written 45426392 spots for SRR30509053.sra
Read 45426391 spots for SRR30509053.sra
Written 45426391 spots for SRR30509053.sra
SRR ids: ['SRR30509053.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_kn__o0ds
SRR30509053.sra spots: 181705565
blocks: [[1, 45426391], [45426392, 90852782], [90852783, 136279173], [136279174, 181705565]]
SRR30509053 file size 40091716
SRR30509053 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 4 -o SRR30509053 SRR30509053_1.fastq SRR30509053_2.fastq
Input file:	SRR30509053_1.fastq
Paired file:	SRR30509053_2.fastq
trimmed:	SRR30509053-trimmed-pair1.fastq, SRR30509053-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	4
Wed Oct 15 13:47:37 2025 >> started

Wed Oct 15 14:03:30 2025 >> done (952.759s)
181705565 read pairs processed; of these:
    17491 ( 0.01%) short read pairs filtered out after trimming by size control
    27027 ( 0.01%) empty read pairs filtered out after trimming by size control
181661047 (99.98%) read pairs available; of these:
  2011334 ( 1.11%) trimmed read pairs available after processing
179649713 (98.89%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      199	  0.00%
 19	      195	  0.00%
 20	      171	  0.00%
 21	      154	  0.00%
 22	      165	  0.00%
 23	      181	  0.00%
 24	      266	  0.00%
 25	      291	  0.00%
 26	      232	  0.00%
 27	      272	  0.00%
 28	      354	  0.00%
 29	      345	  0.00%
 30	      439	  0.00%
 31	      524	  0.00%
 32	      628	  0.00%
 33	      669	  0.00%
 34	      791	  0.00%
 35	     1035	  0.00%
 36	     1144	  0.00%
 37	     1387	  0.00%
 38	     1561	  0.00%
 39	     1940	  0.00%
 40	     2395	  0.00%
 41	     3141	  0.00%
 42	     3813	  0.00%
 43	     4228	  0.00%
 44	     4801	  0.00%
 45	     5340	  0.00%
 46	     6735	  0.00%
 47	     8554	  0.00%
 48	     9708	  0.01%
 49	    13124	  0.01%
 50	    13751	  0.01%
 51	    16630	  0.01%
 52	    21132	  0.01%
 53	    19288	  0.01%
 54	    21837	  0.01%
 55	    21816	  0.01%
 56	    22982	  0.01%
 57	    28795	  0.02%
 58	    35747	  0.02%
 59	    37860	  0.02%
 60	    44069	  0.02%
 61	    50201	  0.03%
 62	    58834	  0.03%
 63	    60734	  0.03%
 64	    69417	  0.04%
 65	    72744	  0.04%
 66	    76064	  0.04%
 67	    82122	  0.05%
 68	    92530	  0.05%
 69	   109677	  0.06%
 70	   119109	  0.07%
 71	   133173	  0.07%
 72	   154286	  0.08%
 73	   183853	  0.10%
 74	   389901	  0.21%
 75	179649713	 98.89%
181661047 reads passed initial QC


criterion=sequence-density
sequence-density=0.34
sequence-density-rank=1
fanout-score=3.08
fanout-score-rank=17
prefix-density=0.33
prefix-fanout=3.1
sequence=CGCTGCTCTGTGAAACCAGCCTCACGC


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=31
fanout-score=86.25
fanout-score-rank=1
prefix-density=1.74
prefix-fanout=1.0
sequence=CGGTTAATAATCCCGCTGGTACTGAAACCATTAAAAGAACACCCAATAATTTGTTAGGCACTGTACGAAGTATTTGAAATACAGGAAAGAAATACCATTCAGGTAATATTTCCAAAGGAGTTGCAAAAGGATCCGCAGGTTCACCAATCATTGATGGTTCTAAAACCGCTAAGCCTACGTTACAGGCAATGGTACCAAGAATAACTACTGGAAAAATATATAAAAGGTCGTTGGGCCATGCGGGTTCCCCGTAATAATTGTGACCCATACCTTTAGCTAATTTAGCTCGTAATACAGGATCATTCAAATCTGGTTTTTTTGTTACTCCCATAATCCATTATCTCTTCATAGAATTCCCTTCAACTTTTTATGTCAAAAAAATAATAAAATATTGTGGAGTTGAAGGGAAATATCCAAATACATGTTTTATTTTTTTAATAATCCATATTTATAGCAATAAAATACTATCGTTCCTTCACCAAGTAATAAGTAAGTACAAATACCTAGAATC


criterion=sequence-density
sequence-density=0.55
sequence-density-rank=1
fanout-score=1.95
fanout-score-rank=35
prefix-density=0.52
prefix-fanout=2.0
sequence=TTGGGTTCAAAGC


criterion=fanout-score
sequence-density=0.06
sequence-density-rank=22
fanout-score=51.55
fanout-score-rank=1
prefix-density=3.02
prefix-fanout=1.0
sequence=CTCCCTTTTTTA
SRR30509053 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Oct 15 14:03:55
                             Started mapping on |	Oct 15 14:03:55
                                    Finished on |	Oct 15 14:39:56
       Mapping speed, Million of reads per hour |	302.63

                          Number of input reads |	181661047
                      Average input read length |	149
                                    UNIQUE READS:
                   Uniquely mapped reads number |	172967583
                        Uniquely mapped reads % |	95.21%
                          Average mapped length |	149.66
                       Number of splices: Total |	27829711
            Number of splices: Annotated (sjdb) |	27653165
                       Number of splices: GT/AG |	27176450
                       Number of splices: GC/AG |	276966
                       Number of splices: AT/AC |	263123
               Number of splices: Non-canonical |	113172
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.34
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.09
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	3585719
             % of reads mapped to multiple loci |	1.97%
        Number of reads mapped to too many loci |	3675
             % of reads mapped to too many loci |	0.00%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.81%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	5113211	5113211	5113211
N_multimapping	3585719	3585719	3585719
N_noFeature	4282202	167435796	6222106
N_ambiguous	5124187	94785	1522674
UnstrandedReadsAssigned:163561194 PositiveStrandReadsAssigned:5437002 NegativeStrandReadsAssigned:165222803
Dataset is classified negative stranded
MeadianReadLen=75 20thPercentileLength=75 echo kmer=71
SRR30509053 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 48,359
[index] number of k-mers: 45,567,218
[index] number of equivalence classes: 93,973
[quant] running in paired-end mode
[quant] will process pair 1: SRR30509053-trimmed-pair1.fastq
                             SRR30509053-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 181,661,047 reads, 167,230,867 reads pseudoaligned
[quant] estimated average fragment length: 159.135
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,310 rounds

  48360 SRR30509053.ke.tsv
  32833 SRR30509053.se.tsv
  81193 total
==> SRR30509053.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AT1G19090.1	2509	2350.87	0	0
AT1G18320.1	699	541.322	47.3083	0.360786
AT5G11100.1	1709	1550.87	130	0.346049
AT4G35335.1	1280	1121.87	1560	5.74053
AT1G60930.1	3876	3717.87	510	0.566297
AT1G17000.1	2351	2192.87	0	0
AT3G55270.1	3130	2971.87	2581	3.58531
AT4G16150.1	3181	3022.87	4252	5.80687
AT3G18150.1	1370	1211.87	0	0

==> SRR30509053.se.tsv <==
AT1G01010	471
AT1G01020	602
AT1G03987	0
AT1G01030	3
AT1G01040	1358
AT1G03993	1
AT1G01046	7
AT1G01050	3718
AT1G03997	1
AT1G01060	9612
SRR30509053 completed mapping pipeline successfully
