Starting /dee2/code/volunteer_pipeline.sh SRR31913494
    current disk space = 1809374052
    free memory = 61637672 
SRR31913494 SRAfilesize
f1bd0c66c1acb0f60cabc37e7880ba2e  SRR31913494.sra
SRR31913494.sra file validated
SRR31913494 is paired end
SRR31913494 is conventional basespace
SRR31913494 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31913494_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.4505	37.0	37.0	37.0	37.0	37.0
2	36.29225	37.0	37.0	37.0	37.0	37.0
3	36.4485	37.0	37.0	37.0	37.0	37.0
4	36.4625	37.0	37.0	37.0	37.0	37.0
5	36.453	37.0	37.0	37.0	37.0	37.0
6	36.477	37.0	37.0	37.0	37.0	37.0
7	36.418	37.0	37.0	37.0	37.0	37.0
8	36.562	37.0	37.0	37.0	37.0	37.0
9	36.448	37.0	37.0	37.0	37.0	37.0
10-14	36.500750000000004	37.0	37.0	37.0	37.0	37.0
15-19	36.488	37.0	37.0	37.0	37.0	37.0
20-24	36.4322	37.0	37.0	37.0	37.0	37.0
25-29	36.367000000000004	37.0	37.0	37.0	37.0	37.0
30-34	36.3194	37.0	37.0	37.0	37.0	37.0
35-39	36.194399999999995	37.0	37.0	37.0	37.0	37.0
40-44	36.1875	37.0	37.0	37.0	37.0	37.0
45-49	36.094100000000005	37.0	37.0	37.0	37.0	37.0
50-54	35.9743	37.0	37.0	37.0	37.0	37.0
55-59	36.0364	37.0	37.0	37.0	37.0	37.0
60-64	35.887	37.0	37.0	37.0	37.0	37.0
65-69	35.77290000000001	37.0	37.0	37.0	37.0	37.0
70-74	35.5451	37.0	37.0	37.0	37.0	37.0
75-79	35.5486	37.0	37.0	37.0	37.0	37.0
80-84	35.60549999999999	37.0	37.0	37.0	37.0	37.0
85-89	35.415499999999994	37.0	37.0	37.0	37.0	37.0
90-94	35.45140000000001	37.0	37.0	37.0	37.0	37.0
95-99	35.3013	37.0	37.0	37.0	37.0	37.0
100-104	35.2454	37.0	37.0	37.0	34.6	37.0
105-109	35.326	37.0	37.0	37.0	37.0	37.0
110-114	35.0392	37.0	37.0	37.0	27.4	37.0
115-119	34.85889999999999	37.0	37.0	37.0	25.0	37.0
120-124	35.0042	37.0	37.0	37.0	25.0	37.0
125-129	34.92719999999999	37.0	37.0	37.0	25.0	37.0
130-134	34.568799999999996	37.0	37.0	37.0	25.0	37.0
135-139	34.487700000000004	37.0	37.0	37.0	25.0	37.0
140-144	34.743700000000004	37.0	37.0	37.0	25.0	37.0
145-149	34.8283	37.0	37.0	37.0	25.0	37.0
150	34.4655	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	3.0
24	4.0
25	4.0
26	22.0
27	35.0
28	55.0
29	59.0
30	77.0
31	83.0
32	97.0
33	127.0
34	167.0
35	379.0
36	2709.0
37	178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.674999999999997	13.725000000000001	9.55	53.05
2	28.750313047833707	17.856248434760833	36.83946907087403	16.55396944653143
3	21.775	21.725	26.125	30.375000000000004
4	23.9	30.925000000000004	19.75	25.424999999999997
5	25.7	28.775000000000002	21.75	23.775
6	22.675	31.65	23.575	22.1
7	18.793793793793796	15.44044044044044	40.81581581581582	24.94994994994995
8	19.975	18.0	28.749999999999996	33.275
9	26.400000000000002	16.725	29.475	27.400000000000002
10-14	25.416270813540677	24.681234061703087	23.266163308165407	26.63633181659083
15-19	27.72	23.369999999999997	23.27	25.64
20-24	27.944999999999997	23.45	22.775000000000002	25.83
25-29	27.21	23.31	22.865	26.615
30-34	27.43	21.97	23.06	27.54
35-39	27.055	23.49	21.855	27.6
40-44	28.28	22.755	23.095	25.869999999999997
45-49	26.35	23.29	23.79	26.57
50-54	26.595000000000002	23.97	23.415	26.02
55-59	27.705000000000002	23.95	22.79	25.555
60-64	27.415	22.0	24.75	25.835
65-69	26.735	23.59	23.135	26.540000000000003
70-74	26.5	23.155	22.415	27.93
75-79	28.194999999999997	22.43	22.215	27.16
80-84	27.439999999999998	21.815	23.41	27.334999999999997
85-89	28.33	23.24	22.37	26.06
90-94	27.305	23.49	23.175	26.029999999999998
95-99	27.115000000000002	23.87	22.285	26.729999999999997
100-104	26.75	23.925	22.62	26.705000000000002
105-109	27.41	22.85	22.48	27.26
110-114	27.310000000000002	22.075	23.04	27.575
115-119	26.900000000000002	23.494999999999997	22.375	27.229999999999997
120-124	26.950000000000003	23.095	21.55	28.405
125-129	28.310000000000002	22.105	21.675	27.91
130-134	26.384999999999998	22.175	23.89	27.55
135-139	27.49	24.14	21.245	27.125
140-144	26.71	23.990000000000002	20.990000000000002	28.310000000000002
145-149	27.24	23.225	21.97	27.565
150	28.849999999999998	25.374999999999996	19.8	25.974999999999998
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	1.5
32	2.0
33	1.0
34	0.5
35	9.0
36	33.0
37	29.5
38	16.0
39	22.0
40	26.5
41	24.5
42	35.5
43	40.0
44	49.5
45	81.5
46	99.0
47	140.0
48	152.5
49	152.5
50	170.0
51	191.0
52	224.5
53	253.5
54	259.0
55	267.0
56	270.5
57	260.5
58	260.0
59	201.0
60	158.5
61	145.0
62	96.0
63	67.0
64	50.5
65	29.0
66	25.0
67	24.5
68	18.5
69	18.5
70	25.0
71	23.0
72	16.0
73	12.0
74	10.0
75	6.5
76	2.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.17500000000000002
3	0.0
4	0.0
5	0.0
6	0.0
7	0.1
8	0.0
9	0.0
10-14	0.005
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.4
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.85540069686411	38.375
2	18.118466898954704	20.8
3	7.447735191637631	12.825000000000001
4	2.8745644599303137	6.6000000000000005
5	1.4372822299651569	4.125
6	1.0017421602787455	3.45
7	0.6968641114982579	2.8000000000000003
8	0.3048780487804878	1.4000000000000001
9	0.17421602787456447	0.8999999999999999
>10	1.088850174216028	8.725
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCC	26	0.65	No Hit
GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA	22	0.5499999999999999	No Hit
GTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGA	21	0.525	No Hit
CTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCC	20	0.5	No Hit
GTTAGTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGT	19	0.475	No Hit
CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGG	16	0.4	No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT	15	0.375	No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA	14	0.35000000000000003	No Hit
CAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTC	14	0.35000000000000003	No Hit
CCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAGTCTGGAGAA	13	0.325	No Hit
CTCCGATTCACGGAGTAAGTAAAATAACGTTAAAAGTAGTGGTATTTCAC	13	0.325	No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG	13	0.325	No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC	13	0.325	No Hit
CCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCC	12	0.3	No Hit
CGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAAA	12	0.3	No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC	11	0.27499999999999997	No Hit
CGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCA	11	0.27499999999999997	No Hit
CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGC	11	0.27499999999999997	No Hit
ACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGAT	11	0.27499999999999997	No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGAC	11	0.27499999999999997	No Hit
CTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCG	11	0.27499999999999997	No Hit
CTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	10	0.25	No Hit
CGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATT	10	0.25	No Hit
ATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAA	10	0.25	No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG	10	0.25	No Hit
CTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAAGCGCCATC	9	0.22499999999999998	No Hit
CGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTT	9	0.22499999999999998	No Hit
CTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTT	9	0.22499999999999998	No Hit
CGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAG	9	0.22499999999999998	No Hit
CCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA	8	0.2	No Hit
AGAAATTTGAATGATGCGTCGCCAGCACAGAGGCCATGCGATCCGTCGAG	8	0.2	No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT	8	0.2	No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC	8	0.2	No Hit
GTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTAT	8	0.2	No Hit
CGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAACTCG	8	0.2	No Hit
ATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAAC	8	0.2	No Hit
CTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTT	7	0.17500000000000002	No Hit
CTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCG	7	0.17500000000000002	No Hit
CTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC	7	0.17500000000000002	No Hit
CGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCC	7	0.17500000000000002	No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA	7	0.17500000000000002	No Hit
GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA	7	0.17500000000000002	No Hit
TTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCA	7	0.17500000000000002	No Hit
CTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCC	7	0.17500000000000002	No Hit
CAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATTCCT	7	0.17500000000000002	No Hit
CCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTT	7	0.17500000000000002	No Hit
CCCCTACCGATGCATTTTGACATCCCACAGCTTCGGCAGATCGCTTAGCC	7	0.17500000000000002	No Hit
CCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAAGCGCCAT	7	0.17500000000000002	No Hit
ATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTTA	7	0.17500000000000002	No Hit
CGGACGTCTTCGGCGTTCGAATTGTAGTCTGGAGAAGCGTCCTCAGCGAC	7	0.17500000000000002	No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT	7	0.17500000000000002	No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTA	7	0.17500000000000002	No Hit
CACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTAC	6	0.15	No Hit
CTCGCTTCGGCGGGTCGATCCGGGCGGAGGACATTGTCAGGTGGGGAGTT	6	0.15	No Hit
CGGAGATTCCCGAATAGGTTAACCTTTTGAACTGCTGCTGAATCCATGGG	6	0.15	No Hit
CTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTT	6	0.15	No Hit
GTTAGGGGTGAAATGCCACTCGAACCCAGAGCTAGCTGGTTCTCCCCGAA	6	0.15	No Hit
GGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTT	6	0.15	No Hit
CTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTT	6	0.15	No Hit
GTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCC	6	0.15	No Hit
ATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCAACGAC	6	0.15	No Hit
CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCATTTTGCCGACTTCC	6	0.15	No Hit
CGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCG	6	0.15	No Hit
CAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAA	6	0.15	No Hit
GTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCGACGAAA	6	0.15	No Hit
CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGT	6	0.15	No Hit
CTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCA	6	0.15	No Hit
CTCCGTCACCCGTTACCACCATGGTAGGCCACTATCCTACCATCGAAAGT	6	0.15	No Hit
CTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTG	6	0.15	No Hit
GTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCTCTGGG	6	0.15	No Hit
TGGGAGTATAGCATGGGTTACTTCAGCGCCGTAGCGCCTGGTACTCGAAC	6	0.15	No Hit
CTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCT	6	0.15	No Hit
AATCAACTAGCCCCGAAAATGGATGGCGCTTAAGCGCGCGACCTATACCC	6	0.15	No Hit
TATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCAA	6	0.15	No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAG	6	0.15	No Hit
CTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACAC	5	0.125	No Hit
AGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGG	5	0.125	No Hit
CGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGTAAT	5	0.125	No Hit
CGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGCGCGGCGGT	5	0.125	No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGA	5	0.125	No Hit
CAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAG	5	0.125	No Hit
CGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTT	5	0.125	No Hit
CTTGCAGTCAAGCTCCCTTCTGCCTTTGCACTCGAGGGCCAATCTCCGTC	5	0.125	No Hit
TCCCCTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAG	5	0.125	No Hit
CAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACT	5	0.125	No Hit
CGAAGCGGAAGCGAGAATGTCGGCTTGAGTAACGCAAACATTGGTGAGAA	5	0.125	No Hit
GTTAAAATTCCGGAACCGGGACGTGGCGGTTGACGGCAACGTTAGGGAGT	5	0.125	No Hit
AAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGC	5	0.125	No Hit
GTGAAATGCCACTCGAACCCAGAGCTAGCTGGTTCTCCCCGAAATGCGTT	5	0.125	No Hit
TGACAATGTCCTCCGCCCGGATCGACCCGCCGAAGCGAGCCTTGGGACCA	5	0.125	No Hit
CTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCTG	5	0.125	No Hit
AATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAA	5	0.125	No Hit
CTTTTCTGACACCTCTAGCTTCAAATTCCGAAGGTCTAAAGGATCGATAG	5	0.125	No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG	5	0.125	No Hit
TGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCGACGAAAT	5	0.125	No Hit
CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGGAC	5	0.125	No Hit
GTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATAC	5	0.125	No Hit
CAGGCCTCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCG	5	0.125	No Hit
CCCTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCT	5	0.125	No Hit
TAAAAAGCTCGTAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTTGG	5	0.125	No Hit
CGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCA	5	0.125	No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTA	5	0.125	No Hit
GTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTC	5	0.125	No Hit
CTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAG	5	0.125	No Hit
ATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAA	5	0.125	No Hit
CTCAAACTTCCTTGGCCTAAACGGCCATAGTCCCTCTAAGAAGCCGGCCG	5	0.125	No Hit
CTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCATAG	5	0.125	No Hit
TAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTTAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.05	0.0	0.0	0.0	0.0
66-67	0.075	0.0	0.0	0.0	0.0
68-69	0.075	0.0	0.0	0.0	0.0
70-71	0.075	0.0	0.0	0.0	0.0
72-73	0.075	0.0	0.0	0.0	0.0
74-75	0.075	0.0	0.0	0.0	0.0
76-77	0.0875	0.0	0.0	0.0	0.0
78-79	0.1	0.0	0.0	0.0	0.0
80-81	0.1	0.0	0.0	0.0	0.0
82-83	0.1375	0.0	0.0	0.0	0.0
84-85	0.175	0.0	0.0	0.0	0.0
86-87	0.2	0.0	0.0	0.0	0.0
88-89	0.2	0.0	0.0	0.0	0.0
90-91	0.2	0.0	0.0	0.0	0.0
92-93	0.21250000000000002	0.0	0.0	0.0	0.0
94-95	0.25	0.0	0.0	0.0	0.0
96-97	0.275	0.0	0.0	0.0	0.0
98-99	0.30000000000000004	0.0	0.0	0.0	0.0
100-101	0.3875	0.0	0.0	0.0	0.0
102-103	0.4375	0.0	0.0	0.0	0.0
104-105	0.475	0.0	0.0	0.0	0.0
106-107	0.5625	0.0	0.0	0.0	0.0
108-109	0.65	0.0	0.0	0.0	0.0
110-111	0.7124999999999999	0.0	0.0	0.0	0.0
112-113	0.7625	0.0	0.0	0.0	0.0
114-115	0.8875	0.0	0.0	0.0	0.0
116-117	0.9875	0.0	0.0	0.0	0.0
118-119	1.15	0.0	0.0	0.0	0.0
120-121	1.3625	0.0	0.0	0.0	0.0
122-123	1.475	0.0	0.0	0.0	0.0
124-125	1.6	0.0	0.0	0.0	0.0
126-127	1.6749999999999998	0.0	0.0	0.0	0.0
128-129	1.75	0.0	0.0	0.0	0.0
130-131	1.95	0.0	0.0	0.0	0.0
132-133	2.1375	0.0	0.0	0.0	0.0
134-135	2.35	0.0	0.0	0.0	0.0
136-137	2.575	0.0	0.0	0.0	0.0
138	2.775	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCAAA	10	0.006973645	144.0	6
GCCTTCA	10	0.006973645	144.0	4
AGCCAAT	10	0.006973645	144.0	6
CCTTCAA	10	0.006973645	144.0	5
CAAAGTT	10	0.006973645	144.0	9
CGGCCTT	10	0.006973645	144.0	2
>>END_MODULE
SRR31913494 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31913494_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.093	37.0	37.0	37.0	37.0	37.0
2	36.185	37.0	37.0	37.0	37.0	37.0
3	36.3815	37.0	37.0	37.0	37.0	37.0
4	36.297	37.0	37.0	37.0	37.0	37.0
5	36.2585	37.0	37.0	37.0	37.0	37.0
6	36.305	37.0	37.0	37.0	37.0	37.0
7	36.1315	37.0	37.0	37.0	37.0	37.0
8	36.3315	37.0	37.0	37.0	37.0	37.0
9	36.269	37.0	37.0	37.0	37.0	37.0
10-14	36.2909	37.0	37.0	37.0	37.0	37.0
15-19	36.267700000000005	37.0	37.0	37.0	37.0	37.0
20-24	36.2685	37.0	37.0	37.0	37.0	37.0
25-29	36.269	37.0	37.0	37.0	37.0	37.0
30-34	36.234300000000005	37.0	37.0	37.0	37.0	37.0
35-39	36.14640000000001	37.0	37.0	37.0	37.0	37.0
40-44	36.060900000000004	37.0	37.0	37.0	37.0	37.0
45-49	36.0771	37.0	37.0	37.0	37.0	37.0
50-54	36.0289	37.0	37.0	37.0	37.0	37.0
55-59	35.9716	37.0	37.0	37.0	37.0	37.0
60-64	35.8617	37.0	37.0	37.0	37.0	37.0
65-69	35.819500000000005	37.0	37.0	37.0	37.0	37.0
70-74	35.6813	37.0	37.0	37.0	37.0	37.0
75-79	35.7432	37.0	37.0	37.0	37.0	37.0
80-84	35.607	37.0	37.0	37.0	37.0	37.0
85-89	35.5138	37.0	37.0	37.0	37.0	37.0
90-94	35.334500000000006	37.0	37.0	37.0	34.6	37.0
95-99	35.4356	37.0	37.0	37.0	37.0	37.0
100-104	35.4148	37.0	37.0	37.0	34.6	37.0
105-109	35.283	37.0	37.0	37.0	32.2	37.0
110-114	35.4184	37.0	37.0	37.0	34.6	37.0
115-119	34.985899999999994	37.0	37.0	37.0	25.0	37.0
120-124	34.8651	37.0	37.0	37.0	25.0	37.0
125-129	34.9368	37.0	37.0	37.0	27.4	37.0
130-134	34.93920000000001	37.0	37.0	37.0	25.0	37.0
135-139	34.6853	37.0	37.0	37.0	25.0	37.0
140-144	34.2324	37.0	37.0	37.0	25.0	37.0
145-149	34.57899999999999	37.0	37.0	37.0	25.0	37.0
150	34.4705	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.0
22	1.0
23	1.0
24	5.0
25	6.0
26	11.0
27	18.0
28	28.0
29	29.0
30	50.0
31	76.0
32	72.0
33	121.0
34	300.0
35	847.0
36	2377.0
37	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.5	13.625000000000002	11.1	52.775000000000006
2	28.525	17.150000000000002	37.175000000000004	17.150000000000002
3	19.625	21.675	27.525	31.175000000000004
4	24.825	30.099999999999998	20.1	24.975
5	27.200000000000003	30.15	20.8	21.85
6	19.625	32.0	25.05	23.325000000000003
7	20.025000000000002	14.625	39.25	26.1
8	20.775	18.0	29.599999999999998	31.624999999999996
9	25.1	17.224999999999998	29.375	28.299999999999997
10-14	24.86	24.895	23.275000000000002	26.97
15-19	27.935	23.69	23.225	25.15
20-24	27.11	23.955000000000002	22.945	25.990000000000002
25-29	26.889999999999997	23.32	22.84	26.950000000000003
30-34	27.405	22.425	22.634999999999998	27.534999999999997
35-39	27.77	22.58	22.215	27.435
40-44	27.825	22.88	23.285	26.009999999999998
45-49	26.540000000000003	23.165	23.494999999999997	26.8
50-54	26.96	23.445	23.93	25.665
55-59	26.650000000000002	23.474999999999998	23.345	26.529999999999998
60-64	27.395000000000003	22.035	23.555	27.015
65-69	27.01	23.064999999999998	22.91	27.015
70-74	26.275	23.3	22.32	28.105000000000004
75-79	27.894999999999996	21.735	23.165	27.205000000000002
80-84	27.315	21.634999999999998	23.23	27.82
85-89	27.675	22.795	22.48	27.05
90-94	27.284999999999997	22.925	23.465	26.325
95-99	26.935	23.155	22.97	26.939999999999998
100-104	26.91	23.044999999999998	23.45	26.595000000000002
105-109	27.79	22.32	22.485	27.405
110-114	27.36	22.23	22.6	27.810000000000002
115-119	26.96	22.985	22.91	27.145000000000003
120-124	27.075	22.61	22.735	27.58
125-129	28.225	21.89	22.05	27.834999999999997
130-134	25.64	22.255	24.465	27.639999999999997
135-139	27.229999999999997	23.025000000000002	22.215	27.529999999999998
140-144	26.810000000000002	23.435	21.535	28.22
145-149	27.325	23.5	22.165000000000003	27.01
150	28.375	23.200000000000003	21.3	27.125
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	0.5
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.5
33	0.5
34	2.0
35	13.5
36	35.0
37	32.0
38	22.0
39	26.0
40	25.5
41	21.5
42	37.5
43	51.0
44	60.5
45	76.0
46	83.5
47	124.5
48	132.0
49	129.0
50	166.0
51	185.0
52	245.0
53	284.5
54	254.0
55	264.0
56	272.5
57	260.0
58	238.5
59	196.5
60	162.0
61	139.5
62	104.5
63	75.5
64	51.0
65	23.5
66	22.5
67	28.0
68	26.0
69	36.0
70	40.5
71	24.5
72	12.0
73	5.0
74	5.0
75	3.0
76	1.5
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.75
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.48484848484848	39.550000000000004
2	16.83982683982684	19.45
3	6.58008658008658	11.4
4	3.116883116883117	7.199999999999999
5	1.0822510822510822	3.125
6	1.2554112554112553	4.35
7	0.9090909090909091	3.675
8	0.38961038961038963	1.7999999999999998
9	0.2597402597402597	1.35
>10	1.0822510822510822	8.1
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTAGTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGT	22	0.5499999999999999	No Hit
CTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCC	20	0.5	No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG	20	0.5	No Hit
CCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCC	18	0.44999999999999996	No Hit
GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA	14	0.35000000000000003	No Hit
CCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAGTCTGGAGAA	14	0.35000000000000003	No Hit
CTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC	13	0.325	No Hit
CTTGAACCGATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCGG	13	0.325	No Hit
CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGG	13	0.325	No Hit
ATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAAC	13	0.325	No Hit
GTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGA	12	0.3	No Hit
CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGT	12	0.3	No Hit
CTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTT	12	0.3	No Hit
CTTTATCACTGAGCGGTCATTTAGGGGCCTTAGCTGGTGATCCGGGCTGT	11	0.27499999999999997	No Hit
CAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAG	11	0.27499999999999997	No Hit
CTTTAATGGGCGAACAGCCCAACCCTTGGAACATACTACAGCCCCAGGTG	11	0.27499999999999997	No Hit
CCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA	11	0.27499999999999997	No Hit
CTCCGATTCACGGAGTAAGTAAAATAACGTTAAAAGTAGTGGTATTTCAC	11	0.27499999999999997	No Hit
AGAAATTTGAATGATGCGTCGCCAGCACAGAGGCCATGCGATCCGTCGAG	11	0.27499999999999997	No Hit
ATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAA	11	0.27499999999999997	No Hit
CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGC	11	0.27499999999999997	No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC	10	0.25	No Hit
CAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAA	10	0.25	No Hit
CTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTG	10	0.25	No Hit
CGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAG	10	0.25	No Hit
CCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCC	9	0.22499999999999998	No Hit
CTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAAGCGCCATC	9	0.22499999999999998	No Hit
CAAACCCCGACTTATGGAAGGGACGCATTTATTAGATAAAAGGTCGACGC	9	0.22499999999999998	No Hit
ATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTTA	9	0.22499999999999998	No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT	9	0.22499999999999998	No Hit
CTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCG	9	0.22499999999999998	No Hit
CCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGA	8	0.2	No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT	8	0.2	No Hit
GGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTT	8	0.2	No Hit
CGGGCTGTTTCCCTCTCGACGATGAAGCTTATCCCCCACCGTCTCACTGG	8	0.2	No Hit
GTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCCCGA	8	0.2	No Hit
CTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGG	8	0.2	No Hit
GGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGA	8	0.2	No Hit
CTCAAACTTCCTTGGCCTAAACGGCCATAGTCCCTCTAAGAAGCCGGCCG	8	0.2	No Hit
CTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCATAG	8	0.2	No Hit
CTCCCTTCTGCCTTTGCACTCGAGGGCCAATCTCCGTCCGGCCCGAGGAA	7	0.17500000000000002	No Hit
CTTGCAGTCAAGCTCCCTTCTGCCTTTGCACTCGAGGGCCAATCTCCGTC	7	0.17500000000000002	No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA	7	0.17500000000000002	No Hit
CAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTC	7	0.17500000000000002	No Hit
CTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCG	7	0.17500000000000002	No Hit
CTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	7	0.17500000000000002	No Hit
ATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCAACGAC	7	0.17500000000000002	No Hit
CGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATT	7	0.17500000000000002	No Hit
GTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTA	7	0.17500000000000002	No Hit
CGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCG	7	0.17500000000000002	No Hit
CTTTTCTGACACCTCTAGCTTCAAATTCCGAAGGTCTAAAGGATCGATAG	7	0.17500000000000002	No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG	7	0.17500000000000002	No Hit
TGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCGACGAAAT	7	0.17500000000000002	No Hit
CGGCCAGTGAGACGGTGGGGGATAAGCTTCATCGTCGAGAGGGAAACAGC	7	0.17500000000000002	No Hit
CGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCT	7	0.17500000000000002	No Hit
CGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCA	7	0.17500000000000002	No Hit
TGGGAGTATAGCATGGGTTACTTCAGCGCCGTAGCGCCTGGTACTCGAAC	7	0.17500000000000002	No Hit
CCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAAGCGCCAT	7	0.17500000000000002	No Hit
GTCGAGTTATCATGAATCATCAGAGCAACGGGCAGAGCCCGCGTCGACCT	7	0.17500000000000002	No Hit
CTTCGGCGTTCGAATTGTAGTCTGGAGAAGCGTCCTCAGCGACGGACCGG	7	0.17500000000000002	No Hit
TGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAACTCGTTG	7	0.17500000000000002	No Hit
ATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGGAAGCCGGGTTA	6	0.15	No Hit
GATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAACTCGT	6	0.15	No Hit
AAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGA	6	0.15	No Hit
CCTGGTCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAA	6	0.15	No Hit
CCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAACCCTTGGGTTTT	6	0.15	No Hit
GGGAGCTTGACTGCAAGACCCACCCGTCGAGCAGGGACGAAAGTCGGCCT	6	0.15	No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGA	6	0.15	No Hit
GTTTCCCTCTCGACGATGAAGCTTATCCCCCACCGTCTCACTGGCCGACC	6	0.15	No Hit
GTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCT	6	0.15	No Hit
AATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTT	6	0.15	No Hit
TTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCA	6	0.15	No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT	6	0.15	No Hit
CTGCATTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAG	6	0.15	No Hit
CTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCC	6	0.15	No Hit
CGCCCTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAG	6	0.15	No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC	6	0.15	No Hit
GTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCTCTGGG	6	0.15	No Hit
CGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAAT	6	0.15	No Hit
ACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGAT	6	0.15	No Hit
CCCCTTTCGAAGTTCGCGCTTAAGCGCTATCAGACGGGTTTCCCCCGACT	6	0.15	No Hit
CTCCCCTACCGATGCATTTTGACATCCCACAGCTTCGGCAGATCGCTTAG	6	0.15	No Hit
GTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTAT	6	0.15	No Hit
TATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCAA	6	0.15	No Hit
GTTCCGAACCCGTCGGCTGTCAGCGGACTGCTCGAGCTGCTTCCGCGGCG	6	0.15	No Hit
CTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTTAACGC	6	0.15	No Hit
CCCGGATCGACCCGCCGAAGCGAGCCTTGGGACCAAAAACAGGGGTTGTA	6	0.15	No Hit
GGATAAGCTTCATCGTCGAGAGGGAAACAGCCCGGATCACCAGCTAAGGC	6	0.15	No Hit
CAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAA	6	0.15	No Hit
TAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCGACGAAATGCTTCGGG	6	0.15	No Hit
TAGAAACTGTCTACCTGAGACTGTCCCTTGGCCCGTAGGTCCTGACACAA	5	0.125	No Hit
CGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAAC	5	0.125	No Hit
TGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCA	5	0.125	No Hit
GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT	5	0.125	No Hit
GTTCATCTTCGGCGCAAGAGCGCTCGATCAGTGAGCTATTACGCACTCTT	5	0.125	No Hit
GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA	5	0.125	No Hit
GTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACGAGAACAGAAAT	5	0.125	No Hit
CCCCATGCTACTCGGGTCAGAGCATAAGCTAGTGATGCTTTCGGCTACTG	5	0.125	No Hit
TGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCTCGGGG	5	0.125	No Hit
CTCTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGC	5	0.125	No Hit
GTGAAATGCCACTCGAACCCAGAGCTAGCTGGTTCTCCCCGAAATGCGTT	5	0.125	No Hit
CTGTAGGTGGCTTTTTAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGG	5	0.125	No Hit
CGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAAA	5	0.125	No Hit
CTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTA	5	0.125	No Hit
CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG	5	0.125	No Hit
GGGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAGT	5	0.125	No Hit
GTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAAT	5	0.125	No Hit
ATTAAATCAGTTATAGTTTGTTTGATGGTAACTACTACTCGGATAACCGT	5	0.125	No Hit
TACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCG	5	0.125	No Hit
CGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCG	5	0.125	No Hit
CTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCG	5	0.125	No Hit
CTCACGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGAACATACTAC	5	0.125	No Hit
CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT	5	0.125	No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTA	5	0.125	No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.05	0.0	0.0	0.0	0.0
66-67	0.075	0.0	0.0	0.0	0.0
68-69	0.075	0.0	0.0	0.0	0.0
70-71	0.075	0.0	0.0	0.0	0.0
72-73	0.075	0.0	0.0	0.0	0.0
74-75	0.0875	0.0	0.0	0.0	0.0
76-77	0.1125	0.0	0.0	0.0	0.0
78-79	0.125	0.0	0.0	0.0	0.0
80-81	0.125	0.0	0.0	0.0	0.0
82-83	0.16249999999999998	0.0	0.0	0.0	0.0
84-85	0.2	0.0	0.0	0.0	0.0
86-87	0.225	0.0	0.0	0.0	0.0
88-89	0.225	0.0	0.0	0.0	0.0
90-91	0.225	0.0	0.0	0.0	0.0
92-93	0.2375	0.0	0.0	0.0	0.0
94-95	0.275	0.0	0.0	0.0	0.0
96-97	0.3125	0.0	0.0	0.0	0.0
98-99	0.35	0.0	0.0	0.0	0.0
100-101	0.44999999999999996	0.0	0.0	0.0	0.0
102-103	0.5125	0.0	0.0	0.0	0.0
104-105	0.55	0.0	0.0	0.0	0.0
106-107	0.6375	0.0	0.0	0.0	0.0
108-109	0.725	0.0	0.0	0.0	0.0
110-111	0.7875000000000001	0.0	0.0	0.0	0.0
112-113	0.8375	0.0	0.0	0.0	0.0
114-115	0.9624999999999999	0.0	0.0	0.0	0.0
116-117	1.0375	0.0	0.0	0.0	0.0
118-119	1.2	0.0	0.0	0.0	0.0
120-121	1.3875	0.0	0.0	0.0	0.0
122-123	1.5	0.0	0.0	0.0	0.0
124-125	1.6375000000000002	0.0	0.0	0.0	0.0
126-127	1.75	0.0	0.0	0.0	0.0
128-129	1.9	0.0	0.0	0.0	0.0
130-131	2.1125	0.0	0.0	0.0	0.0
132-133	2.3125	0.0	0.0	0.0	0.0
134-135	2.5125	0.0	0.0	0.0	0.0
136-137	2.7249999999999996	0.0	0.0	0.0	0.0
138	2.925	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTAT	10	0.006973645	144.0	1
TAGCCTT	35	0.0034045284	61.714283	9
GTATTTA	35	0.0034045284	61.714283	4
TTTAGCC	35	0.0034045284	61.714283	7
TATTTAG	35	0.0034045284	61.714283	5
TTAGCCT	35	0.0034045284	61.714283	8
ATTTAGC	35	0.0034045284	61.714283	6
>>END_MODULE
Read 6499246 spots for SRR31913494.sra
Written 6499246 spots for SRR31913494.sra
Read 6499248 spots for SRR31913494.sra
Written 6499248 spots for SRR31913494.sra
Read 6499246 spots for SRR31913494.sra
Written 6499246 spots for SRR31913494.sra
Read 6499246 spots for SRR31913494.sra
Written 6499246 spots for SRR31913494.sra
SRR ids: ['SRR31913494.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_cfsvmuno
SRR31913494.sra spots: 25996986
blocks: [[1, 6499246], [6499247, 12998492], [12998493, 19497738], [19497739, 25996986]]
SRR31913494 file size 9546824
SRR31913494 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 4 -o SRR31913494 SRR31913494_1.fastq SRR31913494_2.fastq
Input file:	SRR31913494_1.fastq
Paired file:	SRR31913494_2.fastq
trimmed:	SRR31913494-trimmed-pair1.fastq, SRR31913494-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	4
Wed Oct  1 11:58:01 2025 >> started

Wed Oct  1 11:59:11 2025 >> done (69.975s)
25996986 read pairs processed; of these:
      78 ( 0.00%) short read pairs filtered out after trimming by size control
      77 ( 0.00%) empty read pairs filtered out after trimming by size control
25996831 (100.00%) read pairs available; of these:
 1072307 ( 4.12%) trimmed read pairs available after processing
24924524 (95.88%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 19	       3	  0.00%
 20	       3	  0.00%
 21	       2	  0.00%
 22	       8	  0.00%
 23	       5	  0.00%
 24	      13	  0.00%
 25	       8	  0.00%
 26	      23	  0.00%
 27	      15	  0.00%
 28	      16	  0.00%
 29	      28	  0.00%
 30	      22	  0.00%
 31	      35	  0.00%
 32	      36	  0.00%
 33	      44	  0.00%
 34	      30	  0.00%
 35	      34	  0.00%
 36	      50	  0.00%
 37	      43	  0.00%
 38	      49	  0.00%
 39	      86	  0.00%
 40	     119	  0.00%
 41	     145	  0.00%
 42	     150	  0.00%
 43	     165	  0.00%
 44	     143	  0.00%
 45	     151	  0.00%
 46	     139	  0.00%
 47	     152	  0.00%
 48	     151	  0.00%
 49	     170	  0.00%
 50	     263	  0.00%
 51	     265	  0.00%
 52	     343	  0.00%
 53	     331	  0.00%
 54	     365	  0.00%
 55	     357	  0.00%
 56	     292	  0.00%
 57	     285	  0.00%
 58	     294	  0.00%
 59	     310	  0.00%
 60	     450	  0.00%
 61	     513	  0.00%
 62	     641	  0.00%
 63	     602	  0.00%
 64	     618	  0.00%
 65	     582	  0.00%
 66	     653	  0.00%
 67	     600	  0.00%
 68	     589	  0.00%
 69	     598	  0.00%
 70	     790	  0.00%
 71	     839	  0.00%
 72	    1045	  0.00%
 73	    1134	  0.00%
 74	    1105	  0.00%
 75	    1226	  0.00%
 76	    1185	  0.00%
 77	    1243	  0.00%
 78	    1109	  0.00%
 79	    1229	  0.00%
 80	    1216	  0.00%
 81	    1481	  0.01%
 82	    1892	  0.01%
 83	    1941	  0.01%
 84	    2110	  0.01%
 85	    2287	  0.01%
 86	    2214	  0.01%
 87	    2216	  0.01%
 88	    2104	  0.01%
 89	    2208	  0.01%
 90	    2373	  0.01%
 91	    3056	  0.01%
 92	    3310	  0.01%
 93	    3483	  0.01%
 94	    3957	  0.02%
 95	    4699	  0.02%
 96	    4953	  0.02%
 97	    4699	  0.02%
 98	    5070	  0.02%
 99	    4935	  0.02%
100	    4869	  0.02%
101	    6273	  0.02%
102	    5853	  0.02%
103	    6522	  0.03%
104	    6817	  0.03%
105	    7701	  0.03%
106	    7608	  0.03%
107	    7294	  0.03%
108	    8318	  0.03%
109	    8108	  0.03%
110	    8394	  0.03%
111	    8725	  0.03%
112	   10073	  0.04%
113	    9700	  0.04%
114	    9978	  0.04%
115	   12851	  0.05%
116	   11986	  0.05%
117	   12185	  0.05%
118	   12288	  0.05%
119	   13717	  0.05%
120	   13659	  0.05%
121	   14125	  0.05%
122	   16218	  0.06%
123	   14733	  0.06%
124	   16075	  0.06%
125	   17725	  0.07%
126	   17981	  0.07%
127	   18147	  0.07%
128	   20135	  0.08%
129	   19849	  0.08%
130	   20642	  0.08%
131	   21981	  0.08%
132	   21405	  0.08%
133	   26308	  0.10%
134	   26594	  0.10%
135	   29149	  0.11%
136	   30426	  0.12%
137	   33307	  0.13%
138	   31421	  0.12%
139	   31296	  0.12%
140	   29632	  0.11%
141	   30803	  0.12%
142	   33661	  0.13%
143	   31605	  0.12%
144	   37201	  0.14%
145	   40724	  0.16%
146	   37359	  0.14%
147	   41198	  0.16%
148	   45280	  0.17%
149	   42310	  0.16%
150	24924524	 95.88%
25996831 reads passed initial QC


criterion=sequence-density
sequence-density=4.67
sequence-density-rank=1
fanout-score=3.23
fanout-score-rank=20
prefix-density=4.67
prefix-fanout=3.2
sequence=GCGCTTAAGCGC


criterion=fanout-score
sequence-density=0.11
sequence-density-rank=29
fanout-score=16.00
fanout-score-rank=1
prefix-density=1.34
prefix-fanout=1.4
sequence=AGCCCGGGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTGCTCCGTTCCGCATCCGACCAGGACGCATCGCCGGCCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAACGCCGAAGACGTCCGATTTTCAAGCTGGGCTCTTCCCGGTTCGCTCGCCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCT


criterion=sequence-density
sequence-density=4.67
sequence-density-rank=1
fanout-score=3.17
fanout-score-rank=19
prefix-density=4.68
prefix-fanout=3.2
sequence=GCGCTTAAGCGC


criterion=fanout-score
sequence-density=0.11
sequence-density-rank=29
fanout-score=15.18
fanout-score-rank=1
prefix-density=1.24
prefix-fanout=1.4
sequence=AGCCCGGGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTGCTCCGTTCCGCATCCGACCAGGACGCATCGCCGGCCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAACGCCGAAGACGTCCGATTTTCAAGCTGGGCTCTTCCCGGTTCGCTCGCCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 4 -x GCGCTTAAGCGC -y GCGCTTAAGCGC -o SRR31913494 SRR31913494_1.fastq SRR31913494_2.fastq
Input file:	SRR31913494_1.fastq
Paired file:	SRR31913494_2.fastq
trimmed:	SRR31913494-trimmed-pair1.fastq, SRR31913494-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GCGCTTAAGCGC
-- paired 3' end adapter sequence (-y):	GCGCTTAAGCGC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	4
Wed Oct  1 12:00:34 2025 >> started

Wed Oct  1 12:01:01 2025 >> done (27.210s)
15598099 read pairs processed; of these:
    3048 ( 0.02%) short read pairs filtered out after trimming by size control
     163 ( 0.00%) empty read pairs filtered out after trimming by size control
15594888 (99.98%) read pairs available; of these:
       4 ( 0.00%) trimmed read pairs available after processing
15594884 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 19	       2	  0.00%
 20	       2	  0.00%
 21	       1	  0.00%
 22	       5	  0.00%
 23	       3	  0.00%
 24	       8	  0.00%
 25	       4	  0.00%
 26	      17	  0.00%
 27	      11	  0.00%
 28	       8	  0.00%
 29	      14	  0.00%
 30	      16	  0.00%
 31	      15	  0.00%
 32	      20	  0.00%
 33	      27	  0.00%
 34	      20	  0.00%
 35	      20	  0.00%
 36	      32	  0.00%
 37	      30	  0.00%
 38	      23	  0.00%
 39	      55	  0.00%
 40	      71	  0.00%
 41	      87	  0.00%
 42	      93	  0.00%
 43	     106	  0.00%
 44	      78	  0.00%
 45	      82	  0.00%
 46	      82	  0.00%
 47	      89	  0.00%
 48	      75	  0.00%
 49	      98	  0.00%
 50	     165	  0.00%
 51	     157	  0.00%
 52	     210	  0.00%
 53	     216	  0.00%
 54	     218	  0.00%
 55	     197	  0.00%
 56	     179	  0.00%
 57	     179	  0.00%
 58	     172	  0.00%
 59	     184	  0.00%
 60	     259	  0.00%
 61	     313	  0.00%
 62	     370	  0.00%
 63	     359	  0.00%
 64	     372	  0.00%
 65	     318	  0.00%
 66	     371	  0.00%
 67	     364	  0.00%
 68	     360	  0.00%
 69	     362	  0.00%
 70	     475	  0.00%
 71	     506	  0.00%
 72	     606	  0.00%
 73	     655	  0.00%
 74	     674	  0.00%
 75	     695	  0.00%
 76	     713	  0.00%
 77	     719	  0.00%
 78	     664	  0.00%
 79	     757	  0.00%
 80	     700	  0.00%
 81	     926	  0.01%
 82	    1157	  0.01%
 83	    1172	  0.01%
 84	    1235	  0.01%
 85	    1382	  0.01%
 86	    1315	  0.01%
 87	    1334	  0.01%
 88	    1303	  0.01%
 89	    1358	  0.01%
 90	    1443	  0.01%
 91	    1817	  0.01%
 92	    2005	  0.01%
 93	    2064	  0.01%
 94	    2392	  0.02%
 95	    2808	  0.02%
 96	    2953	  0.02%
 97	    2806	  0.02%
 98	    3046	  0.02%
 99	    2926	  0.02%
100	    2914	  0.02%
101	    3801	  0.02%
102	    3477	  0.02%
103	    3919	  0.03%
104	    4111	  0.03%
105	    4541	  0.03%
106	    4520	  0.03%
107	    4380	  0.03%
108	    4987	  0.03%
109	    4815	  0.03%
110	    5141	  0.03%
111	    5210	  0.03%
112	    6072	  0.04%
113	    5838	  0.04%
114	    6009	  0.04%
115	    7648	  0.05%
116	    7249	  0.05%
117	    7315	  0.05%
118	    7409	  0.05%
119	    8216	  0.05%
120	    8232	  0.05%
121	    8496	  0.05%
122	    9802	  0.06%
123	    8771	  0.06%
124	    9670	  0.06%
125	   10657	  0.07%
126	   10702	  0.07%
127	   10955	  0.07%
128	   11920	  0.08%
129	   12005	  0.08%
130	   12342	  0.08%
131	   13126	  0.08%
132	   12816	  0.08%
133	   15823	  0.10%
134	   15793	  0.10%
135	   17459	  0.11%
136	   18216	  0.12%
137	   19954	  0.13%
138	   18840	  0.12%
139	   18823	  0.12%
140	   17888	  0.11%
141	   18466	  0.12%
142	   20243	  0.13%
143	   18884	  0.12%
144	   22357	  0.14%
145	   24401	  0.16%
146	   22347	  0.14%
147	   24838	  0.16%
148	   27226	  0.17%
149	   25242	  0.16%
150	14951897	 95.88%


criterion=sequence-density
sequence-density=4.62
sequence-density-rank=1
fanout-score=3.23
fanout-score-rank=20
prefix-density=4.64
prefix-fanout=3.2
sequence=GCGCTTAAGCGC


criterion=fanout-score
sequence-density=0.11
sequence-density-rank=29
fanout-score=15.95
fanout-score-rank=1
prefix-density=1.31
prefix-fanout=1.4
sequence=AGCCCGGGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTGCTCCGTTCCGCATCCGACCAGGACGCATCGCCGGCCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAACGCCGAAGACGTCCGATTTTCAAGCTGGGCTCTTCCCGGTTCGCTCGCCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCT


criterion=sequence-density
sequence-density=4.63
sequence-density-rank=1
fanout-score=3.16
fanout-score-rank=20
prefix-density=4.65
prefix-fanout=3.1
sequence=GCGCTTAAGCGC


criterion=fanout-score
sequence-density=0.11
sequence-density-rank=29
fanout-score=15.52
fanout-score-rank=1
prefix-density=1.23
prefix-fanout=1.4
sequence=AGCCCGGGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTGCTCCGTTCCGCATCCGACCAGGACGCATCGCCGGCCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAACGCCGAAGACGTCCGATTTTCAAGCTGGGCTCTTCCCGGTTCGCTCGCCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCT
SRR31913494 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Oct 01 12:01:36
                             Started mapping on |	Oct 01 12:01:36
                                    Finished on |	Oct 01 12:05:47
       Mapping speed, Million of reads per hour |	372.82

                          Number of input reads |	25993620
                      Average input read length |	298
                                    UNIQUE READS:
                   Uniquely mapped reads number |	6707955
                        Uniquely mapped reads % |	25.81%
                          Average mapped length |	297.24
                       Number of splices: Total |	213156
            Number of splices: Annotated (sjdb) |	197427
                       Number of splices: GT/AG |	197669
                       Number of splices: GC/AG |	1894
                       Number of splices: AT/AC |	1669
               Number of splices: Non-canonical |	11924
                      Mismatch rate per base, % |	0.44%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.20
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	18903507
             % of reads mapped to multiple loci |	72.72%
        Number of reads mapped to too many loci |	289
             % of reads mapped to too many loci |	0.00%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.47%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	382158	382158	382158
N_multimapping	18903507	18903507	18903507
N_noFeature	4850229	5772778	5773427
N_ambiguous	22893	5694	5464
UnstrandedReadsAssigned:1834833 PositiveStrandReadsAssigned:929483 NegativeStrandReadsAssigned:929064
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR31913494 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 48,359
[index] number of k-mers: 45,567,218
[index] number of equivalence classes: 93,973
[quant] running in paired-end mode
[quant] will process pair 1: SRR31913494-trimmed-pair1.fastq
                             SRR31913494-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 25,993,620 reads, 1,093,367 reads pseudoaligned
[quant] estimated average fragment length: 206.576
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 813 rounds

  48360 SRR31913494.ke.tsv
  32833 SRR31913494.se.tsv
  81193 total
==> SRR31913494.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AT1G19090.1	2509	2303.42	0	0
AT1G18320.1	699	493.424	0	0
AT5G11100.1	1709	1503.42	3	0.255918
AT4G35335.1	1280	1074.42	0	0
AT1G60930.1	3876	3670.42	1	0.0349418
AT1G17000.1	2351	2145.42	0	0
AT3G55270.1	3130	2924.42	26	1.14024
AT4G16150.1	3181	2975.42	28	1.2069
AT3G18150.1	1370	1164.42	0	0

==> SRR31913494.se.tsv <==
AT1G01010	3
AT1G01020	2
AT1G03987	0
AT1G01030	2
AT1G01040	22
AT1G03993	0
AT1G01046	0
AT1G01050	2
AT1G03997	0
AT1G01060	0
SRR31913494 completed mapping pipeline successfully
