Starting /dee2/code/volunteer_pipeline.sh SRR31913496
    current disk space = 268038600
    free memory = 117097300 
SRR31913496 SRAfilesize
5edcd83c5732864053f695f151b10304  SRR31913496.sra
SRR31913496.sra file validated
SRR31913496 is paired end
SRR31913496 is conventional basespace
SRR31913496 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31913496_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.45	37.0	37.0	37.0	37.0	37.0
2	36.364	37.0	37.0	37.0	37.0	37.0
3	36.538	37.0	37.0	37.0	37.0	37.0
4	36.496	37.0	37.0	37.0	37.0	37.0
5	36.5115	37.0	37.0	37.0	37.0	37.0
6	36.558	37.0	37.0	37.0	37.0	37.0
7	36.44575	37.0	37.0	37.0	37.0	37.0
8	36.5435	37.0	37.0	37.0	37.0	37.0
9	36.518	37.0	37.0	37.0	37.0	37.0
10-14	36.4785	37.0	37.0	37.0	37.0	37.0
15-19	36.4896	37.0	37.0	37.0	37.0	37.0
20-24	36.4031	37.0	37.0	37.0	37.0	37.0
25-29	36.3368	37.0	37.0	37.0	37.0	37.0
30-34	36.2548	37.0	37.0	37.0	37.0	37.0
35-39	36.2286	37.0	37.0	37.0	37.0	37.0
40-44	36.1355	37.0	37.0	37.0	37.0	37.0
45-49	36.094500000000004	37.0	37.0	37.0	37.0	37.0
50-54	36.051100000000005	37.0	37.0	37.0	37.0	37.0
55-59	36.052200000000006	37.0	37.0	37.0	37.0	37.0
60-64	35.8394	37.0	37.0	37.0	37.0	37.0
65-69	35.7105	37.0	37.0	37.0	37.0	37.0
70-74	35.477	37.0	37.0	37.0	37.0	37.0
75-79	35.5364	37.0	37.0	37.0	37.0	37.0
80-84	35.50000000000001	37.0	37.0	37.0	37.0	37.0
85-89	35.3972	37.0	37.0	37.0	37.0	37.0
90-94	35.45440000000001	37.0	37.0	37.0	37.0	37.0
95-99	35.3185	37.0	37.0	37.0	37.0	37.0
100-104	35.239	37.0	37.0	37.0	34.6	37.0
105-109	35.2188	37.0	37.0	37.0	32.2	37.0
110-114	35.053399999999996	37.0	37.0	37.0	27.4	37.0
115-119	34.8986	37.0	37.0	37.0	25.0	37.0
120-124	34.9917	37.0	37.0	37.0	25.0	37.0
125-129	34.9501	37.0	37.0	37.0	25.0	37.0
130-134	34.617200000000004	37.0	37.0	37.0	25.0	37.0
135-139	34.5783	37.0	37.0	37.0	25.0	37.0
140-144	34.700399999999995	37.0	37.0	37.0	25.0	37.0
145-149	34.7528	37.0	37.0	37.0	25.0	37.0
150	34.6315	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	5.0
24	7.0
25	6.0
26	29.0
27	40.0
28	46.0
29	63.0
30	59.0
31	69.0
32	105.0
33	128.0
34	155.0
35	395.0
36	2732.0
37	160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.175	13.900000000000002	8.924999999999999	53.0
2	28.031062124248496	17.334669338677354	36.998997995991985	17.635270541082164
3	21.2	21.8	26.85	30.15
4	23.95	30.5	18.55	27.0
5	26.924999999999997	29.65	21.425	22.0
6	21.675	30.725	24.65	22.95
7	19.53965474105579	15.486614961220916	39.979984988741556	24.993745308981737
8	21.425	16.8	30.75	31.025000000000002
9	26.5	18.35	29.225	25.924999999999997
10-14	26.002600260026004	24.307430743074306	23.457345734573458	26.232623262326232
15-19	28.599999999999998	22.935	22.79	25.674999999999997
20-24	27.860000000000003	23.59	22.400000000000002	26.150000000000002
25-29	27.985	22.52	23.405	26.090000000000003
30-34	28.48	21.3	22.48	27.74
35-39	27.18	22.905	22.295	27.62
40-44	28.345	22.384999999999998	22.98	26.290000000000003
45-49	27.41	23.080000000000002	23.244999999999997	26.265
50-54	26.534999999999997	24.175	23.330000000000002	25.96
55-59	26.76	24.38	22.634999999999998	26.224999999999998
60-64	26.76	22.415	23.59	27.235
65-69	26.3	23.645	22.835	27.22
70-74	25.985000000000003	23.575	22.21	28.23
75-79	27.685	22.365	22.46	27.49
80-84	27.235	21.985	22.689999999999998	28.09
85-89	27.42	22.900000000000002	22.325	27.355
90-94	27.35	23.080000000000002	22.634999999999998	26.935
95-99	27.02	23.625	22.650000000000002	26.705000000000002
100-104	26.484999999999996	23.974999999999998	22.975	26.565
105-109	27.435	23.235	22.085	27.245
110-114	27.21	22.445	22.665	27.68
115-119	27.389999999999997	23.29	22.255	27.065
120-124	27.655	22.555	21.75	28.04
125-129	28.28	21.91	21.365000000000002	28.444999999999997
130-134	26.540000000000003	22.16	23.315	27.985
135-139	27.575	23.535	21.3	27.589999999999996
140-144	27.115000000000002	23.630000000000003	20.945	28.310000000000002
145-149	27.089999999999996	23.150000000000002	22.175	27.584999999999997
150	27.05	24.9	21.6	26.450000000000003
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.5
30	1.0
31	0.5
32	0.0
33	1.0
34	1.0
35	7.5
36	28.5
37	27.5
38	17.0
39	22.5
40	21.0
41	22.5
42	38.5
43	41.5
44	45.0
45	65.0
46	94.0
47	135.0
48	143.5
49	146.5
50	165.5
51	176.0
52	219.5
53	289.5
54	283.5
55	263.0
56	281.0
57	242.5
58	227.0
59	208.5
60	159.5
61	154.5
62	120.5
63	71.0
64	45.0
65	28.0
66	18.5
67	16.5
68	21.5
69	30.5
70	34.5
71	25.5
72	17.0
73	13.5
74	11.0
75	9.5
76	6.0
77	1.5
78	0.5
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.2
3	0.0
4	0.0
5	0.0
6	0.0
7	0.075
8	0.0
9	0.0
10-14	0.01
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.425000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.2232746955345	36.15
2	18.718989625620207	20.75
3	7.532701849345963	12.525
4	2.661253946774921	5.8999999999999995
5	2.525935949481281	7.000000000000001
6	0.9021199819576003	3.0
7	0.6765899864682002	2.625
8	0.4510599909788002	2.0
9	0.2255299954894001	1.125
>10	1.0825439783491204	8.924999999999999
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCC	31	0.775	No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG	23	0.575	No Hit
GTTAGTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGT	23	0.575	No Hit
GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA	21	0.525	No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT	19	0.475	No Hit
CCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAGTCTGGAGAA	19	0.475	No Hit
CCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCC	17	0.42500000000000004	No Hit
CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGG	16	0.4	No Hit
CTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTT	16	0.4	No Hit
GTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGA	14	0.35000000000000003	No Hit
CGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGAT	13	0.325	No Hit
CTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCC	13	0.325	No Hit
CGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAG	13	0.325	No Hit
CTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCG	13	0.325	No Hit
GGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTT	12	0.3	No Hit
CCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA	12	0.3	No Hit
GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT	11	0.27499999999999997	No Hit
ATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTTA	11	0.27499999999999997	No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC	10	0.25	No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA	10	0.25	No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG	10	0.25	No Hit
CGGCCAGTGAGACGGTGGGGGATAAGCTTCATCGTCGAGAGGGAAACAGC	10	0.25	No Hit
CTTGAACCGATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCGG	10	0.25	No Hit
GTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCTCTGGG	10	0.25	No Hit
CAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAG	9	0.22499999999999998	No Hit
TCCCCTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAG	9	0.22499999999999998	No Hit
AGAAATTTGAATGATGCGTCGCCAGCACAGAGGCCATGCGATCCGTCGAG	9	0.22499999999999998	No Hit
AATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTT	9	0.22499999999999998	No Hit
CCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTT	9	0.22499999999999998	No Hit
CTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTT	8	0.2	No Hit
CACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTAC	8	0.2	No Hit
CGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATT	8	0.2	No Hit
GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCAC	8	0.2	No Hit
CAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAA	8	0.2	No Hit
CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGA	8	0.2	No Hit
CCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCA	8	0.2	No Hit
CGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAAT	8	0.2	No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT	8	0.2	No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC	8	0.2	No Hit
CTCCCTTCTGCCTTTGCACTCGAGGGCCAATCTCCGTCCGGCCCGAGGAA	7	0.17500000000000002	No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA	7	0.17500000000000002	No Hit
CAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTC	7	0.17500000000000002	No Hit
CCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAACCCTTGGGTTTT	7	0.17500000000000002	No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATA	7	0.17500000000000002	No Hit
CCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAACCCTTGGGTTTTC	7	0.17500000000000002	No Hit
GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA	7	0.17500000000000002	No Hit
GTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCCCGA	7	0.17500000000000002	No Hit
CTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTT	7	0.17500000000000002	No Hit
CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG	7	0.17500000000000002	No Hit
CTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCC	7	0.17500000000000002	No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT	7	0.17500000000000002	No Hit
CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGT	7	0.17500000000000002	No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC	7	0.17500000000000002	No Hit
CCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAAGCGCCAT	7	0.17500000000000002	No Hit
GAAAAGGATTGGCTCTGAGGGCTGGGCTCGGGGGTCCCAGTTCCGAACCC	6	0.15	No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGA	6	0.15	No Hit
ATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCAAC	6	0.15	No Hit
CTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCTCCAAGTGGGCCATTT	6	0.15	No Hit
CTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGA	6	0.15	No Hit
CCCCTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGC	6	0.15	No Hit
CTCCGATTCACGGAGTAAGTAAAATAACGTTAAAAGTAGTGGTATTTCAC	6	0.15	No Hit
ACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAG	6	0.15	No Hit
GTTTGTTTGATGGTAACTACTACTCGGATAACCGTAGTAATTCTAGAGCT	6	0.15	No Hit
CTCAAACTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCAC	6	0.15	No Hit
ATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAA	6	0.15	No Hit
GGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGG	6	0.15	No Hit
CTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCC	6	0.15	No Hit
CCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGC	6	0.15	No Hit
CGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCG	6	0.15	No Hit
CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGC	6	0.15	No Hit
CCTTCCTCTAAATGATAAGGTTTAGTGGACTTCTCGCGACGTCGCGGGCG	6	0.15	No Hit
CTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATT	6	0.15	No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG	6	0.15	No Hit
CGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATT	6	0.15	No Hit
CACCCGTTACCACCATGGTAGGCCACTATCCTACCATCGAAAGTTGATAG	5	0.125	No Hit
CAAGCTTGAGTACGGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAA	5	0.125	No Hit
CAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGAT	5	0.125	No Hit
CACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCAAGG	5	0.125	No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC	5	0.125	No Hit
ATTGGCTCTGAGGGCTGGGCTCGGGGGTCCCAGTTCCGAACCCGTCGGCT	5	0.125	No Hit
CGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGAT	5	0.125	No Hit
CTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAAGCGCCATC	5	0.125	No Hit
GTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTTCCGATC	5	0.125	No Hit
CTCGCTTCGGCGGGTCGATCCGGGCGGAGGACATTGTCAGGTGGGGAGTT	5	0.125	No Hit
CTTCCAGCCGCTGGACCCTACCTCCGGCTGAGCCGTTTCCAGGGTGGGCA	5	0.125	No Hit
CCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGA	5	0.125	No Hit
CCTTGCGTCCTTGAACCGATAACCATCTTTCGGCTAACCTAGCCTCCTCC	5	0.125	No Hit
CAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACT	5	0.125	No Hit
CGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCG	5	0.125	No Hit
CGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGT	5	0.125	No Hit
TGCTCTTCCAGCCGCTGGACCCTACCTCCGGCTGAGCCGTTTCCAGGGTG	5	0.125	No Hit
CTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	5	0.125	No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC	5	0.125	No Hit
GGGAGCTTGACTGCAAGACCCACCCGTCGAGCAGGGACGAAAGTCGGCCT	5	0.125	No Hit
CCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAAT	5	0.125	No Hit
TGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCG	5	0.125	No Hit
CTGTAGGTGGCTTTTTAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGG	5	0.125	No Hit
TAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCT	5	0.125	No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTT	5	0.125	No Hit
CTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCTTAATTGGCCGGGTCGT	5	0.125	No Hit
CGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAAA	5	0.125	No Hit
CTTTGCACTCGAGGGCCAATCTCCGTCCGGCCCGAGGAAACCTTTGCACG	5	0.125	No Hit
TCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCC	5	0.125	No Hit
GTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCGACGAAA	5	0.125	No Hit
GTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTA	5	0.125	No Hit
AAAAAAGCGTCGTGCTGCTAGGCGAAGCGGTGGAGTGCCGCACCCTAGAT	5	0.125	No Hit
GTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATAC	5	0.125	No Hit
GTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAAT	5	0.125	No Hit
CCCTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCT	5	0.125	No Hit
CGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCA	5	0.125	No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG	5	0.125	No Hit
GTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCA	5	0.125	No Hit
CTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTT	5	0.125	No Hit
CTTCAGCGCCGTAGCGCCTGGTACTCGAACATTGGCTCGGGGCATTTTCT	5	0.125	No Hit
CTTGGAACATACTACAGCCCCAGGTGGCGAAGAGCCGACATCGAGGTGCC	5	0.125	No Hit
GGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCC	5	0.125	No Hit
AGCCACCCTTGAAAGAGTGCGTAATAGCTCACTGATCGAGCGCTCTTGCG	5	0.125	No Hit
GGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC	5	0.125	No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGAC	5	0.125	No Hit
CCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA	5	0.125	No Hit
TGGGAGTATAGCATGGGTTACTTCAGCGCCGTAGCGCCTGGTACTCGAAC	5	0.125	No Hit
CTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCT	5	0.125	No Hit
CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC	5	0.125	No Hit
CTCCCCTACCGATGCATTTTGACATCCCACAGCTTCGGCAGATCGCTTAG	5	0.125	No Hit
TATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCAA	5	0.125	No Hit
GTCGAGTTATCATGAATCATCAGAGCAACGGGCAGAGCCCGCGTCGACCT	5	0.125	No Hit
CTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCATAG	5	0.125	No Hit
CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT	5	0.125	No Hit
CGACGAAATGCTTCGGGGAGTTGAAAATAAGCGTAGATCCGGAGATTCCC	5	0.125	No Hit
AGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0125	0.0	0.0	0.0	0.0
42-43	0.025	0.0	0.0	0.0	0.0
44-45	0.025	0.0	0.0	0.0	0.0
46-47	0.025	0.0	0.0	0.0	0.0
48-49	0.025	0.0	0.0	0.0	0.0
50-51	0.025	0.0	0.0	0.0	0.0
52-53	0.025	0.0	0.0	0.0	0.0
54-55	0.025	0.0	0.0	0.0	0.0
56-57	0.025	0.0	0.0	0.0	0.0
58-59	0.025	0.0	0.0	0.0	0.0
60-61	0.025	0.0	0.0	0.0	0.0
62-63	0.025	0.0	0.0	0.0	0.0
64-65	0.025	0.0	0.0	0.0	0.0
66-67	0.025	0.0	0.0	0.0	0.0
68-69	0.05	0.0	0.0	0.0	0.0
70-71	0.05	0.0	0.0	0.0	0.0
72-73	0.0625	0.0	0.0	0.0	0.0
74-75	0.075	0.0	0.0	0.0	0.0
76-77	0.075	0.0	0.0	0.0	0.0
78-79	0.075	0.0	0.0	0.0	0.0
80-81	0.075	0.0	0.0	0.0	0.0
82-83	0.075	0.0	0.0	0.0	0.0
84-85	0.1125	0.0	0.0	0.0	0.0
86-87	0.15	0.0	0.0	0.0	0.0
88-89	0.15	0.0	0.0	0.0	0.0
90-91	0.175	0.0	0.0	0.0	0.0
92-93	0.175	0.0	0.0	0.0	0.0
94-95	0.175	0.0	0.0	0.0	0.0
96-97	0.1875	0.0	0.0	0.0	0.0
98-99	0.23750000000000002	0.0	0.0	0.0	0.0
100-101	0.275	0.0	0.0	0.0	0.0
102-103	0.3375	0.0	0.0	0.0	0.0
104-105	0.5125	0.0	0.0	0.0	0.0
106-107	0.6375	0.0	0.0	0.0	0.0
108-109	0.7625	0.0	0.0	0.0	0.0
110-111	0.9125000000000001	0.0	0.0	0.0	0.0
112-113	1.0	0.0	0.0	0.0	0.0
114-115	1.0875	0.0	0.0	0.0	0.0
116-117	1.2875	0.0	0.0	0.0	0.0
118-119	1.4625	0.0	0.0	0.0	0.0
120-121	1.5750000000000002	0.0	0.0	0.0	0.0
122-123	1.6875	0.0	0.0	0.0	0.0
124-125	1.8	0.0	0.0	0.0	0.0
126-127	1.9375	0.0	0.0	0.0	0.0
128-129	2.1125	0.0	0.0	0.0	0.0
130-131	2.2625	0.0	0.0	0.0	0.0
132-133	2.4875	0.0	0.0	0.0	0.0
134-135	2.8	0.0	0.0	0.0	0.0
136-137	3.125	0.0	0.0	0.0	0.0
138	3.325	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTGG	10	0.006973645	144.0	8
GATGTTC	10	0.006973645	144.0	5
TGTTCTG	10	0.006973645	144.0	7
TTCTGGG	10	0.006973645	144.0	9
CTTAGAT	10	0.006973645	144.0	1
AGATGTT	10	0.006973645	144.0	4
ATGTTCT	10	0.006973645	144.0	6
TAGATGT	10	0.006973645	144.0	3
>>END_MODULE
SRR31913496 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31913496_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.0375	37.0	37.0	37.0	37.0	37.0
2	36.1655	37.0	37.0	37.0	37.0	37.0
3	36.1015	37.0	37.0	37.0	37.0	37.0
4	36.122	37.0	37.0	37.0	37.0	37.0
5	36.1785	37.0	37.0	37.0	37.0	37.0
6	36.171	37.0	37.0	37.0	37.0	37.0
7	36.08	37.0	37.0	37.0	37.0	37.0
8	36.247	37.0	37.0	37.0	37.0	37.0
9	36.095	37.0	37.0	37.0	37.0	37.0
10-14	36.1601	37.0	37.0	37.0	37.0	37.0
15-19	36.1707	37.0	37.0	37.0	37.0	37.0
20-24	36.16	37.0	37.0	37.0	37.0	37.0
25-29	36.144800000000004	37.0	37.0	37.0	37.0	37.0
30-34	36.130100000000006	37.0	37.0	37.0	37.0	37.0
35-39	36.0241	37.0	37.0	37.0	37.0	37.0
40-44	35.9342	37.0	37.0	37.0	37.0	37.0
45-49	35.989999999999995	37.0	37.0	37.0	37.0	37.0
50-54	35.8888	37.0	37.0	37.0	37.0	37.0
55-59	35.8524	37.0	37.0	37.0	37.0	37.0
60-64	35.65689999999999	37.0	37.0	37.0	37.0	37.0
65-69	35.6903	37.0	37.0	37.0	37.0	37.0
70-74	35.4534	37.0	37.0	37.0	37.0	37.0
75-79	35.56139999999999	37.0	37.0	37.0	37.0	37.0
80-84	35.3882	37.0	37.0	37.0	34.6	37.0
85-89	35.2959	37.0	37.0	37.0	29.8	37.0
90-94	35.1754	37.0	37.0	37.0	27.4	37.0
95-99	35.2493	37.0	37.0	37.0	32.2	37.0
100-104	35.26270000000001	37.0	37.0	37.0	29.8	37.0
105-109	35.083	37.0	37.0	37.0	25.0	37.0
110-114	35.175200000000004	37.0	37.0	37.0	27.4	37.0
115-119	34.7808	37.0	37.0	37.0	25.0	37.0
120-124	34.658100000000005	37.0	37.0	37.0	25.0	37.0
125-129	34.746700000000004	37.0	37.0	37.0	25.0	37.0
130-134	34.7083	37.0	37.0	37.0	25.0	37.0
135-139	34.4054	37.0	37.0	37.0	25.0	37.0
140-144	34.0214	37.0	37.0	37.0	25.0	37.0
145-149	34.3592	37.0	37.0	37.0	25.0	37.0
150	34.1215	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.0
22	2.0
23	4.0
24	10.0
25	10.0
26	11.0
27	18.0
28	23.0
29	41.0
30	60.0
31	81.0
32	114.0
33	140.0
34	323.0
35	897.0
36	2220.0
37	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.625	13.0	9.700000000000001	52.675000000000004
2	26.724999999999998	18.425	37.1	17.75
3	21.625	22.975	25.25	30.15
4	23.875	30.175	19.575	26.375
5	26.075	29.025000000000002	22.5	22.400000000000002
6	20.075000000000003	31.775	23.799999999999997	24.349999999999998
7	18.6	15.024999999999999	40.625	25.75
8	20.575	17.150000000000002	28.975	33.300000000000004
9	25.650000000000002	17.775	28.799999999999997	27.775
10-14	25.39	24.37	23.9	26.340000000000003
15-19	27.595	22.99	23.31	26.105
20-24	28.07	22.975	23.16	25.795
25-29	26.979999999999997	23.455000000000002	23.105	26.46
30-34	27.275	21.865000000000002	22.715	28.144999999999996
35-39	27.134999999999998	23.775	21.755	27.334999999999997
40-44	27.950000000000003	22.16	23.22	26.669999999999998
45-49	27.455000000000002	23.189999999999998	22.57	26.784999999999997
50-54	27.255000000000003	23.974999999999998	23.54	25.230000000000004
55-59	27.72	24.005000000000003	22.295	25.979999999999997
60-64	26.655	22.900000000000002	23.685000000000002	26.76
65-69	26.66	23.13	23.465	26.745
70-74	26.165	23.485	22.255	28.095
75-79	27.73	22.345000000000002	22.25	27.675
80-84	27.805000000000003	21.095	23.22	27.88
85-89	28.03	22.99	21.66	27.32
90-94	27.229999999999997	23.400000000000002	22.875	26.495
95-99	26.8	23.515	22.63	27.055
100-104	27.134999999999998	23.005	22.939999999999998	26.919999999999998
105-109	27.855	22.705000000000002	21.78	27.66
110-114	26.740000000000002	22.6	23.155	27.505000000000003
115-119	26.650000000000002	22.650000000000002	23.09	27.61
120-124	27.865000000000002	22.365	22.24	27.529999999999998
125-129	28.470000000000002	22.675	21.099999999999998	27.755000000000003
130-134	27.084999999999997	21.58	24.14	27.195000000000004
135-139	26.790000000000003	23.07	22.57	27.57
140-144	27.375	23.48	20.855	28.29
145-149	27.860000000000003	23.855	21.995	26.290000000000003
150	26.825	25.15	20.974999999999998	27.05
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	0.5
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.5
28	0.5
29	0.0
30	0.5
31	0.5
32	0.0
33	0.0
34	2.5
35	11.5
36	33.5
37	33.0
38	18.5
39	24.5
40	26.5
41	27.0
42	34.0
43	32.0
44	50.0
45	76.5
46	87.5
47	123.0
48	133.0
49	148.0
50	180.5
51	192.0
52	238.0
53	278.0
54	261.0
55	239.0
56	240.0
57	260.0
58	249.0
59	184.0
60	158.5
61	152.5
62	112.0
63	85.5
64	61.0
65	30.0
66	20.0
67	18.5
68	23.0
69	41.5
70	48.5
71	29.5
72	11.5
73	5.0
74	8.0
75	7.0
76	2.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.5482007996446	37.45
2	17.85872945357619	20.1
3	7.019102621057308	11.85
4	3.6428254109284763	8.200000000000001
5	1.6437139049311416	4.625
6	0.8440693025322079	2.85
7	0.7552199022656597	2.9749999999999996
8	0.3998223011994669	1.7999999999999998
9	0.2221235006663705	1.125
>10	1.0661928031985786	9.025
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCC	30	0.75	No Hit
GTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGA	27	0.675	No Hit
CCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCC	25	0.625	No Hit
GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA	23	0.575	No Hit
GTTAGTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGT	20	0.5	No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG	17	0.42500000000000004	No Hit
CCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA	16	0.4	No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT	14	0.35000000000000003	No Hit
CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGG	14	0.35000000000000003	No Hit
CTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTT	14	0.35000000000000003	No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT	14	0.35000000000000003	No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA	13	0.325	No Hit
CTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTT	13	0.325	No Hit
CGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAAA	13	0.325	No Hit
ATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAA	12	0.3	No Hit
GTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCTCTGGG	12	0.3	No Hit
ATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTTA	12	0.3	No Hit
CAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAG	11	0.27499999999999997	No Hit
CAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATTCCT	11	0.27499999999999997	No Hit
CCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCC	10	0.25	No Hit
CAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTC	10	0.25	No Hit
CCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAGTCTGGAGAA	10	0.25	No Hit
CTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCG	10	0.25	No Hit
CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG	10	0.25	No Hit
CTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAAGCGCCATC	9	0.22499999999999998	No Hit
GGATTAACGAGATTCCCACTGTCCCTGTCTACTATCCAGCGAAACCACAG	9	0.22499999999999998	No Hit
CTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC	9	0.22499999999999998	No Hit
CCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCA	9	0.22499999999999998	No Hit
CCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAAGCGCCAT	9	0.22499999999999998	No Hit
CACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTAC	8	0.2	No Hit
GCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTG	8	0.2	No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCT	8	0.2	No Hit
GTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATAC	8	0.2	No Hit
CAAACCCCGACTTATGGAAGGGACGCATTTATTAGATAAAAGGTCGACGC	8	0.2	No Hit
CTTGAACCGATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCGG	8	0.2	No Hit
CGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAA	8	0.2	No Hit
CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGC	8	0.2	No Hit
CGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAAT	8	0.2	No Hit
CCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTA	7	0.17500000000000002	No Hit
CGCATTTATTAGATAAAAGGTCGACGCGGGCTCTGCCCGTTGCTCTGATG	7	0.17500000000000002	No Hit
CTCCGATTCACGGAGTAAGTAAAATAACGTTAAAAGTAGTGGTATTTCAC	7	0.17500000000000002	No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA	7	0.17500000000000002	No Hit
GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA	7	0.17500000000000002	No Hit
GGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAAT	7	0.17500000000000002	No Hit
AATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTT	7	0.17500000000000002	No Hit
CTCAAACTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCAC	7	0.17500000000000002	No Hit
CTGGAAACGGCTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGAGGAATC	7	0.17500000000000002	No Hit
CCAAGCTTGAGTACGGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAA	7	0.17500000000000002	No Hit
GTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGA	7	0.17500000000000002	No Hit
AAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCA	7	0.17500000000000002	No Hit
GGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGC	7	0.17500000000000002	No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG	7	0.17500000000000002	No Hit
CTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCATAG	7	0.17500000000000002	No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC	7	0.17500000000000002	No Hit
CGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAG	7	0.17500000000000002	No Hit
GTGAAATGCGTAGAGATCGGAAAGAACACCAACGGCGAAAGCACTCTGCT	6	0.15	No Hit
AGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGG	6	0.15	No Hit
CGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAAC	6	0.15	No Hit
TGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCA	6	0.15	No Hit
CCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCT	6	0.15	No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGG	6	0.15	No Hit
GGAAAGAACACCAACGGCGAAAGCACTCTGCTGGGCCGACACTGACACTG	6	0.15	No Hit
CCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAACCCTTGGGTTTT	6	0.15	No Hit
GTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGG	6	0.15	No Hit
AGAAATTTGAATGATGCGTCGCCAGCACAGAGGCCATGCGATCCGTCGAG	6	0.15	No Hit
GTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCCCGA	6	0.15	No Hit
GTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAAT	6	0.15	No Hit
CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGT	6	0.15	No Hit
CCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA	6	0.15	No Hit
CGAAAGCTAGGGGAGCGAATGGGATTAGATACCCCAGTAGTCCTAGCCGT	6	0.15	No Hit
CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC	6	0.15	No Hit
CTCCCCTACCGATGCATTTTGACATCCCACAGCTTCGGCAGATCGCTTAG	6	0.15	No Hit
CGGACGTCTTCGGCGTTCGAATTGTAGTCTGGAGAAGCGTCCTCAGCGAC	6	0.15	No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTA	6	0.15	No Hit
GATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAACTCGT	5	0.125	No Hit
CTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACAC	5	0.125	No Hit
ATTGGCTCTGAGGGCTGGGCTCGGGGGTCCCAGTTCCGAACCCGTCGGCT	5	0.125	No Hit
TAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTC	5	0.125	No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGA	5	0.125	No Hit
ATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGA	5	0.125	No Hit
CGGCGTTCGAATTGTAGTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCC	5	0.125	No Hit
CCCAGGAGTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGAT	5	0.125	No Hit
TTCCCCTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAA	5	0.125	No Hit
CAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACT	5	0.125	No Hit
TGGGGCTGCATTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGC	5	0.125	No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATA	5	0.125	No Hit
CCCCTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGC	5	0.125	No Hit
GGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAA	5	0.125	No Hit
CCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCG	5	0.125	No Hit
CTCCCATTTCGCTCGCCGCTACTACGGGAATCGCTTTTGCTTTCTTTTCC	5	0.125	No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC	5	0.125	No Hit
CTTTGTTGAGCCGTCACACGGAATCGAGAGCTCCAAGTGGGCCATTTTTG	5	0.125	No Hit
CGAGTTATCATGAATCATCAGAGCAACGGGCAGAGCCCGCGTCGACCTTT	5	0.125	No Hit
CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGA	5	0.125	No Hit
TTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCA	5	0.125	No Hit
AAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGA	5	0.125	No Hit
AAGCACTCTGCTGGGCCGACACTGACACTGAGAGACGAAAGCTAGGGGAG	5	0.125	No Hit
CGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCA	5	0.125	No Hit
CGGCCAGTGAGACGGTGGGGGATAAGCTTCATCGTCGAGAGGGAAACAGC	5	0.125	No Hit
CTTGACTGCAAGACCCACCCGTCGAGCAGGGACGAAAGTCGGCCTTAGTG	5	0.125	No Hit
CAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGG	5	0.125	No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC	5	0.125	No Hit
ATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAA	5	0.125	No Hit
CTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCG	5	0.125	No Hit
GTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCA	5	0.125	No Hit
CCCTTTCGAAGTTCGCGCTTAAGCGCTATCAGACGGGTTTCCCCCGACTC	5	0.125	No Hit
TATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCAA	5	0.125	No Hit
CTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTTAACGC	5	0.125	No Hit
CTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGAC	5	0.125	No Hit
CAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAA	5	0.125	No Hit
TTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0125	0.0	0.0	0.0	0.0
42-43	0.025	0.0	0.0	0.0	0.0
44-45	0.025	0.0	0.0	0.0	0.0
46-47	0.025	0.0	0.0	0.0	0.0
48-49	0.025	0.0	0.0	0.0	0.0
50-51	0.025	0.0	0.0	0.0	0.0
52-53	0.025	0.0	0.0	0.0	0.0
54-55	0.025	0.0	0.0	0.0	0.0
56-57	0.025	0.0	0.0	0.0	0.0
58-59	0.025	0.0	0.0	0.0	0.0
60-61	0.025	0.0	0.0	0.0	0.0
62-63	0.025	0.0	0.0	0.0	0.0
64-65	0.025	0.0	0.0	0.0	0.0
66-67	0.025	0.0	0.0	0.0	0.0
68-69	0.05	0.0	0.0	0.0	0.0
70-71	0.05	0.0	0.0	0.0	0.0
72-73	0.0625	0.0	0.0	0.0	0.0
74-75	0.075	0.0	0.0	0.0	0.0
76-77	0.075	0.0	0.0	0.0	0.0
78-79	0.075	0.0	0.0	0.0	0.0
80-81	0.075	0.0	0.0	0.0	0.0
82-83	0.075	0.0	0.0	0.0	0.0
84-85	0.1125	0.0	0.0	0.0	0.0
86-87	0.15	0.0	0.0	0.0	0.0
88-89	0.15	0.0	0.0	0.0	0.0
90-91	0.175	0.0	0.0	0.0	0.0
92-93	0.175	0.0	0.0	0.0	0.0
94-95	0.175	0.0	0.0	0.0	0.0
96-97	0.1875	0.0	0.0	0.0	0.0
98-99	0.23750000000000002	0.0	0.0	0.0	0.0
100-101	0.275	0.0	0.0	0.0	0.0
102-103	0.3375	0.0	0.0	0.0	0.0
104-105	0.5125	0.0	0.0	0.0	0.0
106-107	0.6375	0.0	0.0	0.0	0.0
108-109	0.7625	0.0	0.0	0.0	0.0
110-111	0.8875	0.0	0.0	0.0	0.0
112-113	0.975	0.0	0.0	0.0	0.0
114-115	1.0625	0.0	0.0	0.0	0.0
116-117	1.2625	0.0	0.0	0.0	0.0
118-119	1.4375	0.0	0.0	0.0	0.0
120-121	1.5499999999999998	0.0	0.0	0.0	0.0
122-123	1.6875	0.0	0.0	0.0	0.0
124-125	1.8	0.0	0.0	0.0	0.0
126-127	1.95	0.0	0.0	0.0	0.0
128-129	2.1375	0.0	0.0	0.0	0.0
130-131	2.2875	0.0	0.0	0.0	0.0
132-133	2.5625	0.0	0.0	0.0	0.0
134-135	2.9375	0.0	0.0	0.0	0.0
136-137	3.25	0.0	0.0	0.0	0.0
138	3.45	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGAGA	20	1.9452218E-6	144.0	6
AACGAAC	10	0.006973645	144.0	2
TAACGAA	10	0.006973645	144.0	1
GAACGAG	10	0.006973645	144.0	5
ACGAGAT	10	0.006973645	144.0	7
ACGAGAC	10	0.006973645	144.0	7
CGAACGA	10	0.006973645	144.0	4
CGAGATT	10	0.006973645	144.0	8
CGAGACC	10	0.006973645	144.0	8
ACGAACG	10	0.006973645	144.0	3
GAGATTC	10	0.006973645	144.0	9
GAGACCT	10	0.006973645	144.0	9
>>END_MODULE
Read 2784888 spots for SRR31913496.sra
Written 2784888 spots for SRR31913496.sra
Read 2784888 spots for SRR31913496.sra
Written 2784888 spots for SRR31913496.sra
Read 2784888 spots for SRR31913496.sra
Written 2784888 spots for SRR31913496.sra
Read 2784888 spots for SRR31913496.sra
Written 2784888 spots for SRR31913496.sra
Read 2784888 spots for SRR31913496.sra
Written 2784888 spots for SRR31913496.sra
Read 2784888 spots for SRR31913496.sra
Written 2784888 spots for SRR31913496.sra
Read 2784888 spots for SRR31913496.sra
Written 2784888 spots for SRR31913496.sra
Read 2784895 spots for SRR31913496.sra
Written 2784895 spots for SRR31913496.sra
SRR ids: ['SRR31913496.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_vgo935rc
SRR31913496.sra spots: 22279111
blocks: [[1, 2784888], [2784889, 5569776], [5569777, 8354664], [8354665, 11139552], [11139553, 13924440], [13924441, 16709328], [16709329, 19494216], [19494217, 22279111]]
SRR31913496 file size 8179928
SRR31913496 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR31913496 SRR31913496_1.fastq SRR31913496_2.fastq
Input file:	SRR31913496_1.fastq
Paired file:	SRR31913496_2.fastq
trimmed:	SRR31913496-trimmed-pair1.fastq, SRR31913496-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Oct  1 14:23:28 2025 >> started

Wed Oct  1 14:30:59 2025 >> done (451.187s)
22279111 read pairs processed; of these:
      67 ( 0.00%) short read pairs filtered out after trimming by size control
     143 ( 0.00%) empty read pairs filtered out after trimming by size control
22278901 (100.00%) read pairs available; of these:
 1418321 ( 6.37%) trimmed read pairs available after processing
20860580 (93.63%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       3	  0.00%
 19	       6	  0.00%
 20	       9	  0.00%
 21	       4	  0.00%
 22	       3	  0.00%
 23	       4	  0.00%
 24	      11	  0.00%
 25	      10	  0.00%
 26	      20	  0.00%
 27	      12	  0.00%
 28	      17	  0.00%
 29	      33	  0.00%
 30	      26	  0.00%
 31	      29	  0.00%
 32	      46	  0.00%
 33	      62	  0.00%
 34	      49	  0.00%
 35	      51	  0.00%
 36	      61	  0.00%
 37	      87	  0.00%
 38	      98	  0.00%
 39	     144	  0.00%
 40	     205	  0.00%
 41	     244	  0.00%
 42	     241	  0.00%
 43	     268	  0.00%
 44	     242	  0.00%
 45	     245	  0.00%
 46	     240	  0.00%
 47	     256	  0.00%
 48	     310	  0.00%
 49	     303	  0.00%
 50	     433	  0.00%
 51	     401	  0.00%
 52	     561	  0.00%
 53	     580	  0.00%
 54	     595	  0.00%
 55	     537	  0.00%
 56	     484	  0.00%
 57	     534	  0.00%
 58	     481	  0.00%
 59	     506	  0.00%
 60	     742	  0.00%
 61	     902	  0.00%
 62	    1039	  0.00%
 63	     979	  0.00%
 64	     928	  0.00%
 65	     901	  0.00%
 66	     964	  0.00%
 67	     834	  0.00%
 68	     886	  0.00%
 69	     920	  0.00%
 70	    1266	  0.01%
 71	    1241	  0.01%
 72	    1548	  0.01%
 73	    1628	  0.01%
 74	    1655	  0.01%
 75	    1704	  0.01%
 76	    1696	  0.01%
 77	    1983	  0.01%
 78	    1613	  0.01%
 79	    1878	  0.01%
 80	    1809	  0.01%
 81	    2089	  0.01%
 82	    2551	  0.01%
 83	    2722	  0.01%
 84	    3017	  0.01%
 85	    3138	  0.01%
 86	    3012	  0.01%
 87	    3090	  0.01%
 88	    2849	  0.01%
 89	    2946	  0.01%
 90	    3259	  0.01%
 91	    4025	  0.02%
 92	    4688	  0.02%
 93	    4794	  0.02%
 94	    5449	  0.02%
 95	    6022	  0.03%
 96	    6722	  0.03%
 97	    6218	  0.03%
 98	    7046	  0.03%
 99	    6611	  0.03%
100	    6456	  0.03%
101	    8505	  0.04%
102	    7746	  0.03%
103	    8896	  0.04%
104	    9345	  0.04%
105	   10018	  0.04%
106	    9690	  0.04%
107	    9548	  0.04%
108	   10916	  0.05%
109	   10497	  0.05%
110	   11686	  0.05%
111	   11736	  0.05%
112	   13753	  0.06%
113	   12799	  0.06%
114	   13267	  0.06%
115	   16766	  0.08%
116	   16103	  0.07%
117	   16609	  0.07%
118	   16462	  0.07%
119	   18670	  0.08%
120	   18830	  0.08%
121	   19840	  0.09%
122	   22380	  0.10%
123	   19917	  0.09%
124	   21055	  0.09%
125	   22785	  0.10%
126	   24138	  0.11%
127	   24453	  0.11%
128	   27339	  0.12%
129	   26485	  0.12%
130	   27690	  0.12%
131	   29203	  0.13%
132	   28758	  0.13%
133	   34804	  0.16%
134	   34822	  0.16%
135	   39171	  0.18%
136	   40434	  0.18%
137	   43760	  0.20%
138	   39994	  0.18%
139	   40983	  0.18%
140	   39036	  0.18%
141	   39826	  0.18%
142	   44296	  0.20%
143	   41386	  0.19%
144	   48110	  0.22%
145	   53094	  0.24%
146	   47874	  0.21%
147	   52134	  0.23%
148	   57757	  0.26%
149	   52684	  0.24%
150	20860580	 93.63%
22278901 reads passed initial QC


criterion=sequence-density
sequence-density=5.15
sequence-density-rank=1
fanout-score=3.61
fanout-score-rank=17
prefix-density=5.16
prefix-fanout=3.6
sequence=GCGCTTAAGCGC


criterion=fanout-score
sequence-density=0.91
sequence-density-rank=9
fanout-score=20.44
fanout-score-rank=1
prefix-density=5.16
prefix-fanout=3.6
sequence=CGCTTAAGCGCTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCC


criterion=sequence-density
sequence-density=5.12
sequence-density-rank=1
fanout-score=3.53
fanout-score-rank=19
prefix-density=5.13
prefix-fanout=3.5
sequence=GCGCTTAAGCGC


criterion=fanout-score
sequence-density=0.12
sequence-density-rank=29
fanout-score=16.81
fanout-score-rank=1
prefix-density=1.51
prefix-fanout=1.3
sequence=AGCCCGGGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTGCTCCGTTCCGCATCCGACCAGGACGCATCGCCGGCCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAACGCCGAAGACGTCCGATTTTCAAGCTGGGCTCTTCCCGGTTCGCTCGCCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GCGCTTAAGCGC -y GCGCTTAAGCGC -o SRR31913496 SRR31913496_1.fastq SRR31913496_2.fastq
Input file:	SRR31913496_1.fastq
Paired file:	SRR31913496_2.fastq
trimmed:	SRR31913496-trimmed-pair1.fastq, SRR31913496-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GCGCTTAAGCGC
-- paired 3' end adapter sequence (-y):	GCGCTTAAGCGC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Oct  1 14:43:38 2025 >> started

Wed Oct  1 14:46:29 2025 >> done (170.985s)
14852601 read pairs processed; of these:
    3745 ( 0.03%) short read pairs filtered out after trimming by size control
     191 ( 0.00%) empty read pairs filtered out after trimming by size control
14848665 (99.97%) read pairs available; of these:
       6 ( 0.00%) trimmed read pairs available after processing
14848659 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       3	  0.00%
 19	       6	  0.00%
 20	       5	  0.00%
 21	       2	  0.00%
 22	       3	  0.00%
 23	       4	  0.00%
 24	       4	  0.00%
 25	       8	  0.00%
 26	      15	  0.00%
 27	       8	  0.00%
 28	      12	  0.00%
 29	      25	  0.00%
 30	      19	  0.00%
 31	      14	  0.00%
 32	      31	  0.00%
 33	      38	  0.00%
 34	      27	  0.00%
 35	      31	  0.00%
 36	      47	  0.00%
 37	      54	  0.00%
 38	      70	  0.00%
 39	      99	  0.00%
 40	     107	  0.00%
 41	     149	  0.00%
 42	     162	  0.00%
 43	     185	  0.00%
 44	     168	  0.00%
 45	     165	  0.00%
 46	     157	  0.00%
 47	     164	  0.00%
 48	     212	  0.00%
 49	     197	  0.00%
 50	     292	  0.00%
 51	     286	  0.00%
 52	     375	  0.00%
 53	     388	  0.00%
 54	     404	  0.00%
 55	     348	  0.00%
 56	     331	  0.00%
 57	     342	  0.00%
 58	     305	  0.00%
 59	     333	  0.00%
 60	     497	  0.00%
 61	     618	  0.00%
 62	     681	  0.00%
 63	     665	  0.00%
 64	     622	  0.00%
 65	     600	  0.00%
 66	     623	  0.00%
 67	     556	  0.00%
 68	     607	  0.00%
 69	     609	  0.00%
 70	     833	  0.01%
 71	     861	  0.01%
 72	    1008	  0.01%
 73	    1095	  0.01%
 74	    1093	  0.01%
 75	    1148	  0.01%
 76	    1155	  0.01%
 77	    1323	  0.01%
 78	    1099	  0.01%
 79	    1232	  0.01%
 80	    1253	  0.01%
 81	    1394	  0.01%
 82	    1677	  0.01%
 83	    1784	  0.01%
 84	    2065	  0.01%
 85	    2127	  0.01%
 86	    1975	  0.01%
 87	    2078	  0.01%
 88	    1874	  0.01%
 89	    1995	  0.01%
 90	    2179	  0.01%
 91	    2663	  0.02%
 92	    3150	  0.02%
 93	    3127	  0.02%
 94	    3730	  0.03%
 95	    3962	  0.03%
 96	    4441	  0.03%
 97	    4179	  0.03%
 98	    4651	  0.03%
 99	    4379	  0.03%
100	    4265	  0.03%
101	    5654	  0.04%
102	    5152	  0.03%
103	    5855	  0.04%
104	    6139	  0.04%
105	    6694	  0.05%
106	    6572	  0.04%
107	    6355	  0.04%
108	    7317	  0.05%
109	    7025	  0.05%
110	    7735	  0.05%
111	    7871	  0.05%
112	    9227	  0.06%
113	    8478	  0.06%
114	    8990	  0.06%
115	   11087	  0.07%
116	   10688	  0.07%
117	   11062	  0.07%
118	   10980	  0.07%
119	   12405	  0.08%
120	   12679	  0.09%
121	   13218	  0.09%
122	   14792	  0.10%
123	   13086	  0.09%
124	   13894	  0.09%
125	   15171	  0.10%
126	   16047	  0.11%
127	   16227	  0.11%
128	   18334	  0.12%
129	   17621	  0.12%
130	   18445	  0.12%
131	   19491	  0.13%
132	   19192	  0.13%
133	   23138	  0.16%
134	   23089	  0.16%
135	   26097	  0.18%
136	   27047	  0.18%
137	   29009	  0.20%
138	   26637	  0.18%
139	   27277	  0.18%
140	   26038	  0.18%
141	   26694	  0.18%
142	   29688	  0.20%
143	   27463	  0.18%
144	   31969	  0.22%
145	   35427	  0.24%
146	   31928	  0.22%
147	   34626	  0.23%
148	   38698	  0.26%
149	   35022	  0.24%
150	13903867	 93.64%


criterion=sequence-density
sequence-density=5.19
sequence-density-rank=1
fanout-score=3.60
fanout-score-rank=17
prefix-density=5.21
prefix-fanout=3.6
sequence=GCGCTTAAGCGC


criterion=fanout-score
sequence-density=0.12
sequence-density-rank=29
fanout-score=18.01
fanout-score-rank=1
prefix-density=1.59
prefix-fanout=1.3
sequence=AGCCCGGGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTGCTCCGTTCCGCATCCGACCAGGACGCATCGCCGGCCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAACGCCGAAGACGTCCGATTTTCAAGCTGGGCTCTTCCCGGTTCGCTCGCCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCT


criterion=sequence-density
sequence-density=5.14
sequence-density-rank=1
fanout-score=3.55
fanout-score-rank=17
prefix-density=5.16
prefix-fanout=3.5
sequence=GCGCTTAAGCGC


criterion=fanout-score
sequence-density=0.12
sequence-density-rank=28
fanout-score=16.62
fanout-score-rank=1
prefix-density=1.48
prefix-fanout=1.3
sequence=AGCCCGGGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTGCTCCGTTCCGCATCCGACCAGGACGCATCGCCGGCCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAACGCCGAAGACGTCCGATTTTCAAGCTGGGCTCTTCCCGGTTCGCTCGCCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCT
SRR31913496 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Oct 01 14:49:47
                             Started mapping on |	Oct 01 14:49:47
                                    Finished on |	Oct 01 14:53:33
       Mapping speed, Million of reads per hour |	354.82

                          Number of input reads |	22274965
                      Average input read length |	297
                                    UNIQUE READS:
                   Uniquely mapped reads number |	5532903
                        Uniquely mapped reads % |	24.84%
                          Average mapped length |	296.29
                       Number of splices: Total |	56527
            Number of splices: Annotated (sjdb) |	45430
                       Number of splices: GT/AG |	47562
                       Number of splices: GC/AG |	592
                       Number of splices: AT/AC |	323
               Number of splices: Non-canonical |	8050
                      Mismatch rate per base, % |	0.50%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.20
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.03
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	16417341
             % of reads mapped to multiple loci |	73.70%
        Number of reads mapped to too many loci |	310
             % of reads mapped to too many loci |	0.00%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.46%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	324721	324721	324721
N_multimapping	16417341	16417341	16417341
N_noFeature	4237604	4886139	4879212
N_ambiguous	9240	2145	2011
UnstrandedReadsAssigned:1286059 PositiveStrandReadsAssigned:644619 NegativeStrandReadsAssigned:651680
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR31913496 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 48,359
[index] number of k-mers: 45,567,218
[index] number of equivalence classes: 93,973
[quant] running in paired-end mode
[quant] will process pair 1: SRR31913496-trimmed-pair1.fastq
                             SRR31913496-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 22,274,965 reads, 564,682 reads pseudoaligned
[quant] estimated average fragment length: 181.07
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 725 rounds

  48360 SRR31913496.ke.tsv
  32833 SRR31913496.se.tsv
  81193 total
==> SRR31913496.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AT1G19090.1	2509	2328.93	0	0
AT1G18320.1	699	518.93	1	0.241757
AT5G11100.1	1709	1528.93	0	0
AT4G35335.1	1280	1099.93	1	0.114057
AT1G60930.1	3876	3695.93	0	0
AT1G17000.1	2351	2170.93	0	0
AT3G55270.1	3130	2949.93	3	0.127584
AT4G16150.1	3181	3000.93	5	0.209027
AT3G18150.1	1370	1189.93	0	0

==> SRR31913496.se.tsv <==
AT1G01010	0
AT1G01020	0
AT1G03987	0
AT1G01030	0
AT1G01040	7
AT1G03993	0
AT1G01046	0
AT1G01050	1
AT1G03997	0
AT1G01060	0
SRR31913496 completed mapping pipeline successfully
