Starting /dee2/code/volunteer_pipeline.sh SRR31913495
    current disk space = 1809244316
    free memory = 61880576 
SRR31913495 SRAfilesize
622c33ceff0858856f5554f13063169c  SRR31913495.sra
SRR31913495.sra file validated
SRR31913495 is paired end
SRR31913495 is conventional basespace
SRR31913495 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31913495_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.46	37.0	37.0	37.0	37.0	37.0
2	36.173	37.0	37.0	37.0	37.0	37.0
3	36.2835	37.0	37.0	37.0	37.0	37.0
4	36.4665	37.0	37.0	37.0	37.0	37.0
5	36.4095	37.0	37.0	37.0	37.0	37.0
6	36.5085	37.0	37.0	37.0	37.0	37.0
7	36.3615	37.0	37.0	37.0	37.0	37.0
8	36.4175	37.0	37.0	37.0	37.0	37.0
9	36.3905	37.0	37.0	37.0	37.0	37.0
10-14	36.4412	37.0	37.0	37.0	37.0	37.0
15-19	36.4215	37.0	37.0	37.0	37.0	37.0
20-24	36.3562	37.0	37.0	37.0	37.0	37.0
25-29	36.2616	37.0	37.0	37.0	37.0	37.0
30-34	36.197	37.0	37.0	37.0	37.0	37.0
35-39	36.077099999999994	37.0	37.0	37.0	37.0	37.0
40-44	36.0709	37.0	37.0	37.0	37.0	37.0
45-49	35.9798	37.0	37.0	37.0	37.0	37.0
50-54	35.90840000000001	37.0	37.0	37.0	37.0	37.0
55-59	35.8673	37.0	37.0	37.0	37.0	37.0
60-64	35.741	37.0	37.0	37.0	37.0	37.0
65-69	35.470600000000005	37.0	37.0	37.0	37.0	37.0
70-74	35.4255	37.0	37.0	37.0	37.0	37.0
75-79	35.4882	37.0	37.0	37.0	37.0	37.0
80-84	35.407399999999996	37.0	37.0	37.0	37.0	37.0
85-89	35.355000000000004	37.0	37.0	37.0	37.0	37.0
90-94	35.3539	37.0	37.0	37.0	37.0	37.0
95-99	35.2963	37.0	37.0	37.0	34.6	37.0
100-104	35.1459	37.0	37.0	37.0	34.6	37.0
105-109	35.1404	37.0	37.0	37.0	29.8	37.0
110-114	34.848	37.0	37.0	37.0	25.0	37.0
115-119	34.7238	37.0	37.0	37.0	25.0	37.0
120-124	34.81849999999999	37.0	37.0	37.0	25.0	37.0
125-129	34.869	37.0	37.0	37.0	25.0	37.0
130-134	34.578500000000005	37.0	37.0	37.0	25.0	37.0
135-139	34.385999999999996	37.0	37.0	37.0	25.0	37.0
140-144	34.56439999999999	37.0	37.0	37.0	25.0	37.0
145-149	34.661500000000004	37.0	37.0	37.0	25.0	37.0
150	34.437	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.0
23	2.0
24	5.0
25	25.0
26	34.0
27	34.0
28	60.0
29	50.0
30	100.0
31	78.0
32	94.0
33	123.0
34	166.0
35	419.0
36	2662.0
37	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.7	13.675	10.274999999999999	51.349999999999994
2	28.750627195183142	17.41093828399398	37.35574510787757	16.48268941294531
3	20.424999999999997	22.575	25.474999999999998	31.525
4	24.95	30.45	19.8	24.8
5	26.224999999999998	29.549999999999997	22.525000000000002	21.7
6	22.15	31.175000000000004	25.224999999999998	21.45
7	19.459729864932466	15.282641320660332	39.694847423711856	25.56278139069535
8	20.75	17.75	30.125	31.374999999999996
9	24.75	17.349999999999998	30.325000000000003	27.575
10-14	26.1976197619762	23.932393239323932	23.402340234023402	26.467646764676466
15-19	27.415	23.615	24.0	24.97
20-24	27.91	23.380000000000003	22.99	25.72
25-29	26.919999999999998	22.585	23.885	26.61
30-34	27.74	22.035	22.220000000000002	28.005000000000003
35-39	27.544999999999998	22.685	22.895	26.875
40-44	27.955000000000002	22.770000000000003	22.725	26.55
45-49	26.655	23.474999999999998	23.09	26.779999999999998
50-54	27.245	24.33	22.89	25.535000000000004
55-59	27.18	24.065	23.23	25.525
60-64	26.39	22.325	24.57	26.715
65-69	26.38	23.145	23.715	26.76
70-74	26.950000000000003	23.43	21.84	27.779999999999998
75-79	27.63	22.439999999999998	22.925	27.005000000000003
80-84	26.905	21.9	23.01	28.185
85-89	27.345000000000002	23.294999999999998	22.08	27.279999999999998
90-94	26.86	23.46	22.695	26.985
95-99	26.85	23.835	22.325	26.99
100-104	26.884999999999998	23.665	22.900000000000002	26.55
105-109	27.375	24.025	21.654999999999998	26.945000000000004
110-114	26.919999999999998	22.24	23.095	27.744999999999997
115-119	26.619999999999997	23.855	22.735	26.790000000000003
120-124	27.175	22.715	22.61	27.500000000000004
125-129	28.015	22.555	21.625	27.805000000000003
130-134	26.740000000000002	22.155	23.935000000000002	27.169999999999998
135-139	27.77	23.285	21.240000000000002	27.705000000000002
140-144	27.400000000000002	23.9	21.13	27.57
145-149	27.1	23.43	22.125	27.345000000000002
150	28.299999999999997	24.625	20.925	26.150000000000002
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.0
23	1.0
24	0.0
25	0.0
26	0.5
27	0.5
28	0.0
29	0.0
30	0.0
31	1.5
32	2.5
33	1.0
34	1.5
35	12.0
36	32.5
37	36.5
38	29.5
39	29.5
40	28.5
41	25.0
42	33.0
43	44.0
44	64.5
45	83.0
46	90.0
47	128.0
48	137.0
49	133.5
50	142.5
51	164.5
52	224.5
53	279.0
54	270.0
55	261.5
56	261.5
57	234.5
58	247.5
59	229.5
60	180.5
61	153.0
62	117.0
63	77.0
64	42.5
65	20.5
66	15.5
67	18.0
68	21.5
69	27.5
70	31.5
71	24.5
72	12.5
73	5.5
74	7.0
75	7.0
76	5.5
77	2.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.35000000000000003
3	0.0
4	0.0
5	0.0
6	0.0
7	0.05
8	0.0
9	0.0
10-14	0.01
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.35
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.56495204882302	38.175
2	17.698343504795115	20.3
3	7.149084568439407	12.3
4	3.6181342632955533	8.3
5	1.7872711421098517	5.125
6	1.046207497820401	3.5999999999999996
7	0.7846556233653008	3.15
8	0.34873583260680036	1.6
9	0.17436791630340018	0.8999999999999999
>10	0.8282476024411508	6.550000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCC	25	0.625	No Hit
GTTAGTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGT	20	0.5	No Hit
CCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCC	19	0.475	No Hit
GTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGA	17	0.42500000000000004	No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA	17	0.42500000000000004	No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG	17	0.42500000000000004	No Hit
GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA	14	0.35000000000000003	No Hit
CGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAAA	14	0.35000000000000003	No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT	13	0.325	No Hit
CAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAG	12	0.3	No Hit
CAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAA	12	0.3	No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT	11	0.27499999999999997	No Hit
CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGG	11	0.27499999999999997	No Hit
CCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCC	10	0.25	No Hit
CGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTT	10	0.25	No Hit
CCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAGTCTGGAGAA	10	0.25	No Hit
CGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATT	10	0.25	No Hit
GTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCCCGA	10	0.25	No Hit
ATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAA	10	0.25	No Hit
CCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAACCCTTGGGTTTT	9	0.22499999999999998	No Hit
GGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTT	9	0.22499999999999998	No Hit
CTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	9	0.22499999999999998	No Hit
CTCAAACTTCCTTGGCCTAAACGGCCATAGTCCCTCTAAGAAGCCGGCCG	9	0.22499999999999998	No Hit
CTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAAGCGCCATC	8	0.2	No Hit
AGAAATTTGAATGATGCGTCGCCAGCACAGAGGCCATGCGATCCGTCGAG	8	0.2	No Hit
CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG	8	0.2	No Hit
CTTGAACCGATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCGG	8	0.2	No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTA	8	0.2	No Hit
CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGC	8	0.2	No Hit
TGGGAGTATAGCATGGGTTACTTCAGCGCCGTAGCGCCTGGTACTCGAAC	8	0.2	No Hit
ATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAAC	8	0.2	No Hit
ATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGATA	7	0.17500000000000002	No Hit
CACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTAC	7	0.17500000000000002	No Hit
CTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCG	7	0.17500000000000002	No Hit
CTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCA	7	0.17500000000000002	No Hit
CTCCGATTCACGGAGTAAGTAAAATAACGTTAAAAGTAGTGGTATTTCAC	7	0.17500000000000002	No Hit
ATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGG	7	0.17500000000000002	No Hit
CGACATTCTCGCTTCCGCTTCGTCCACCGCCGCTCGCGCGGTTGCTTCCC	7	0.17500000000000002	No Hit
CTTGAGTACGGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCG	7	0.17500000000000002	No Hit
GTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATAC	7	0.17500000000000002	No Hit
GTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGC	7	0.17500000000000002	No Hit
CGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCT	7	0.17500000000000002	No Hit
CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGT	7	0.17500000000000002	No Hit
CTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCC	7	0.17500000000000002	No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGAC	7	0.17500000000000002	No Hit
CCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAAGCGCCAT	7	0.17500000000000002	No Hit
GTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATC	7	0.17500000000000002	No Hit
CTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCATAG	7	0.17500000000000002	No Hit
CTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCG	7	0.17500000000000002	No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT	6	0.15	No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCAC	6	0.15	No Hit
CGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGAT	6	0.15	No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGA	6	0.15	No Hit
GGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAGTC	6	0.15	No Hit
CTTTAATGGGCGAACAGCCCAACCCTTGGAACATACTACAGCCCCAGGTG	6	0.15	No Hit
CTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC	6	0.15	No Hit
CAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCTCTGGGTT	6	0.15	No Hit
ATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCAACGAC	6	0.15	No Hit
CTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGAC	6	0.15	No Hit
GTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATAC	6	0.15	No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT	6	0.15	No Hit
CTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCG	6	0.15	No Hit
CTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCC	6	0.15	No Hit
CTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTT	6	0.15	No Hit
CGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCA	6	0.15	No Hit
TGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAGGGG	6	0.15	No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC	6	0.15	No Hit
CCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCG	6	0.15	No Hit
CTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTT	6	0.15	No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG	6	0.15	No Hit
CTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGTCCTACCTATT	6	0.15	No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC	6	0.15	No Hit
CGACGAAATGCTTCGGGGAGTTGAAAATAAGCGTAGATCCGGAGATTCCC	6	0.15	No Hit
CTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTT	5	0.125	No Hit
TGAAAATCGGACGTCTTCGGCGTTCGAATTGTAGTCTGGAGAAGCGTCCT	5	0.125	No Hit
CAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATG	5	0.125	No Hit
GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT	5	0.125	No Hit
CCCAAGCAGTGGGAGGAGCCCTGGGCTCTGACCGCGTGCCTGTTGAAGAA	5	0.125	No Hit
GGCAGGCAAGAGACAACCTGGCGAACTGAAACATCTTAGTAGCCAGAGGA	5	0.125	No Hit
GGCTTTTTAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGG	5	0.125	No Hit
CCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCT	5	0.125	No Hit
GTTTAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGA	5	0.125	No Hit
GAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAACTCGTTGA	5	0.125	No Hit
CGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTC	5	0.125	No Hit
TCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTG	5	0.125	No Hit
CCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA	5	0.125	No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGA	5	0.125	No Hit
CTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTT	5	0.125	No Hit
CTTCCCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCT	5	0.125	No Hit
TGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTC	5	0.125	No Hit
GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA	5	0.125	No Hit
GTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCT	5	0.125	No Hit
GGCTGCATTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGAC	5	0.125	No Hit
TATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATT	5	0.125	No Hit
CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGA	5	0.125	No Hit
CGAGAATGTCGGCTTGAGTAACGCAAACATTGGTGAGAATCCAATGCCCC	5	0.125	No Hit
CGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCA	5	0.125	No Hit
CCGGAATCGAACCCTAATTCTCCGTCACCCGTTACCACCATGGTAGGCCA	5	0.125	No Hit
CAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGG	5	0.125	No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA	5	0.125	No Hit
CTCCGCACTTGGCTACCCAGCGTTTACCGTGGGCACGATAACTGGTACAC	5	0.125	No Hit
CTTGCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGTCATTTCACAAA	5	0.125	No Hit
CCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACG	5	0.125	No Hit
CCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTT	5	0.125	No Hit
CCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGG	5	0.125	No Hit
CTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCT	5	0.125	No Hit
GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTCACACCT	5	0.125	No Hit
TACCGATGCATTTTGACATCCCACAGCTTCGGCAGATCGCTTAGCCCCGT	5	0.125	No Hit
GTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTT	5	0.125	No Hit
TATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCAA	5	0.125	No Hit
CGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGCTCTCGATT	5	0.125	No Hit
CAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGG	5	0.125	No Hit
TGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAACTCGTTG	5	0.125	No Hit
TAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTTAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.025	0.0	0.0	0.0	0.0
80-81	0.037500000000000006	0.0	0.0	0.0	0.0
82-83	0.0625	0.0	0.0	0.0	0.0
84-85	0.0875	0.0	0.0	0.0	0.0
86-87	0.1	0.0	0.0	0.0	0.0
88-89	0.1	0.0	0.0	0.0	0.0
90-91	0.1125	0.0	0.0	0.0	0.0
92-93	0.125	0.0	0.0	0.0	0.0
94-95	0.175	0.0	0.0	0.0	0.0
96-97	0.2875	0.0	0.0	0.0	0.0
98-99	0.3	0.0	0.0	0.0	0.0
100-101	0.3375	0.0	0.0	0.0	0.0
102-103	0.3625	0.0	0.0	0.0	0.0
104-105	0.375	0.0	0.0	0.0	0.0
106-107	0.5	0.0	0.0	0.0	0.0
108-109	0.625	0.0	0.0	0.0	0.0
110-111	0.7	0.0	0.0	0.0	0.0
112-113	0.7625	0.0	0.0	0.0	0.0
114-115	0.825	0.0	0.0	0.0	0.0
116-117	0.8875	0.0	0.0	0.0	0.0
118-119	0.9375	0.0	0.0	0.0	0.0
120-121	0.975	0.0	0.0	0.0	0.0
122-123	1.0	0.0	0.0	0.0	0.0
124-125	1.1	0.0	0.0	0.0	0.0
126-127	1.2125	0.0	0.0	0.0	0.0
128-129	1.35	0.0	0.0	0.0	0.0
130-131	1.4875	0.0	0.0	0.0	0.0
132-133	1.6	0.0	0.0	0.0	0.0
134-135	1.7374999999999998	0.0	0.0	0.0	0.0
136-137	1.85	0.0	0.0	0.0	0.0
138	2.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTTAC	20	3.5153638E-4	109.28164	9
CGGGTTA	20	3.5153638E-4	109.28164	8
CGGAAGC	20	3.5153638E-4	109.28164	1
CCGGGTT	20	3.5153638E-4	109.28164	7
AGCCGGG	20	3.5153638E-4	109.28164	5
GGAAGCC	25	8.537841E-4	87.42531	2
GCCGGGT	30	0.0017612103	72.85442	6
AAGCCGG	35	0.003245912	62.446655	4
GAAGCCG	35	0.00341509	61.66607	3
AGACAGC	40	0.008002726	17.985937	65-69
>>END_MODULE
SRR31913495 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31913495_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.803	37.0	37.0	37.0	37.0	37.0
2	36.022	37.0	37.0	37.0	37.0	37.0
3	36.0125	37.0	37.0	37.0	37.0	37.0
4	35.986	37.0	37.0	37.0	37.0	37.0
5	35.964	37.0	37.0	37.0	37.0	37.0
6	35.9775	37.0	37.0	37.0	37.0	37.0
7	35.9485	37.0	37.0	37.0	37.0	37.0
8	36.005	37.0	37.0	37.0	37.0	37.0
9	35.9085	37.0	37.0	37.0	37.0	37.0
10-14	36.0042	37.0	37.0	37.0	37.0	37.0
15-19	35.977	37.0	37.0	37.0	37.0	37.0
20-24	36.0216	37.0	37.0	37.0	37.0	37.0
25-29	35.991	37.0	37.0	37.0	37.0	37.0
30-34	35.9229	37.0	37.0	37.0	37.0	37.0
35-39	35.839099999999995	37.0	37.0	37.0	37.0	37.0
40-44	35.8366	37.0	37.0	37.0	37.0	37.0
45-49	35.7282	37.0	37.0	37.0	37.0	37.0
50-54	35.6579	37.0	37.0	37.0	37.0	37.0
55-59	35.6334	37.0	37.0	37.0	37.0	37.0
60-64	35.490700000000004	37.0	37.0	37.0	37.0	37.0
65-69	35.47950000000001	37.0	37.0	37.0	37.0	37.0
70-74	35.3768	37.0	37.0	37.0	37.0	37.0
75-79	35.363800000000005	37.0	37.0	37.0	37.0	37.0
80-84	35.147299999999994	37.0	37.0	37.0	27.4	37.0
85-89	35.165000000000006	37.0	37.0	37.0	25.0	37.0
90-94	34.995099999999994	37.0	37.0	37.0	25.0	37.0
95-99	34.99849999999999	37.0	37.0	37.0	25.0	37.0
100-104	35.0013	37.0	37.0	37.0	25.0	37.0
105-109	34.902100000000004	37.0	37.0	37.0	25.0	37.0
110-114	34.9417	37.0	37.0	37.0	25.0	37.0
115-119	34.5955	37.0	37.0	37.0	25.0	37.0
120-124	34.4062	37.0	37.0	37.0	25.0	37.0
125-129	34.379200000000004	37.0	37.0	37.0	25.0	37.0
130-134	34.4027	37.0	37.0	37.0	25.0	37.0
135-139	34.2348	37.0	37.0	37.0	25.0	37.0
140-144	33.741200000000006	37.0	37.0	37.0	25.0	37.0
145-149	34.10000000000001	37.0	37.0	37.0	25.0	37.0
150	33.9895	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	2.0
23	8.0
24	9.0
25	13.0
26	20.0
27	25.0
28	27.0
29	61.0
30	67.0
31	84.0
32	103.0
33	201.0
34	350.0
35	1040.0
36	1965.0
37	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.7	14.274999999999999	9.85	51.175000000000004
2	28.249999999999996	18.475	37.45	15.825
3	21.0	22.6	25.174999999999997	31.225
4	24.55	31.0	18.65	25.8
5	25.45	29.7	21.075	23.775
6	21.65	31.574999999999996	24.075	22.7
7	18.75	15.9	40.825	24.525
8	19.0	17.875	31.275	31.85
9	26.275	17.175	29.275000000000002	27.275
10-14	25.240000000000002	24.255	24.035	26.47
15-19	27.575	23.89	23.31	25.224999999999998
20-24	28.1	23.685000000000002	22.715	25.5
25-29	27.865000000000002	22.884999999999998	22.57	26.68
30-34	27.985	22.6	22.755	26.66
35-39	26.5	23.73	21.84	27.93
40-44	27.845	22.45	23.35	26.355
45-49	27.24	22.97	22.975	26.815
50-54	26.729999999999997	23.7	24.325	25.245
55-59	26.91	23.97	22.75	26.369999999999997
60-64	26.63	22.73	24.2	26.44
65-69	26.26	23.29	23.905	26.545
70-74	26.105	23.26	22.475	28.16
75-79	27.755000000000003	22.205	22.975	27.065
80-84	27.310000000000002	21.47	23.599999999999998	27.62
85-89	27.625	23.57	22.509999999999998	26.295
90-94	26.924999999999997	23.65	23.325000000000003	26.1
95-99	26.38	24.224999999999998	22.665	26.729999999999997
100-104	26.68	23.29	23.405	26.625
105-109	27.339999999999996	22.64	22.49	27.529999999999998
110-114	26.88	22.66	23.305	27.155
115-119	27.355	23.275000000000002	22.37	27.0
120-124	26.91	22.855	22.025	28.21
125-129	27.93	21.87	22.435	27.765
130-134	26.58	22.115000000000002	23.77	27.534999999999997
135-139	26.919999999999998	22.945	22.085	28.050000000000004
140-144	26.055	23.27	21.85	28.825
145-149	27.644999999999996	23.94	21.985	26.43
150	26.875	24.325	21.775	27.025
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	0.5
25	0.0
26	0.0
27	1.0
28	1.5
29	0.5
30	0.5
31	0.5
32	1.0
33	3.5
34	3.5
35	12.5
36	32.5
37	30.0
38	23.0
39	33.5
40	30.0
41	23.0
42	46.0
43	53.0
44	47.5
45	69.0
46	93.0
47	132.0
48	138.0
49	146.0
50	170.5
51	197.0
52	250.0
53	265.0
54	247.5
55	246.0
56	261.5
57	263.5
58	254.0
59	215.0
60	157.0
61	128.0
62	104.0
63	72.5
64	45.0
65	26.0
66	22.5
67	19.0
68	22.0
69	33.0
70	32.5
71	22.0
72	9.5
73	2.5
74	4.0
75	5.0
76	2.5
77	0.5
78	0.5
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.76889848812095	39.800000000000004
2	16.15550755939525	18.7
3	6.7386609071274295	11.700000000000001
4	3.3693304535637147	7.8
5	1.9870410367170626	5.75
6	0.8639308855291578	3.0
7	0.47516198704103674	1.925
8	0.4319654427645789	2.0
9	0.2591792656587473	1.35
>10	0.9503239740820735	7.9750000000000005
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGA	24	0.6	No Hit
GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA	23	0.575	No Hit
GTTAGTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGT	20	0.5	No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT	18	0.44999999999999996	No Hit
CCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCC	17	0.42500000000000004	No Hit
CGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCG	17	0.42500000000000004	No Hit
CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGG	16	0.4	No Hit
CTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTT	15	0.375	No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG	15	0.375	No Hit
GTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCTCTGGG	15	0.375	No Hit
CTCCGATTCACGGAGTAAGTAAAATAACGTTAAAAGTAGTGGTATTTCAC	14	0.35000000000000003	No Hit
CAAACCCCGACTTATGGAAGGGACGCATTTATTAGATAAAAGGTCGACGC	14	0.35000000000000003	No Hit
CTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAAGCGCCATC	12	0.3	No Hit
CTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCC	12	0.3	No Hit
CAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAA	12	0.3	No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT	12	0.3	No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGA	11	0.27499999999999997	No Hit
CCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAGTCTGGAGAA	11	0.27499999999999997	No Hit
CTTGAACCGATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCGG	11	0.27499999999999997	No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT	10	0.25	No Hit
CTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTG	10	0.25	No Hit
CGGACGTCTTCGGCGTTCGAATTGTAGTCTGGAGAAGCGTCCTCAGCGAC	10	0.25	No Hit
CCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCC	9	0.22499999999999998	No Hit
CAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAG	9	0.22499999999999998	No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA	9	0.22499999999999998	No Hit
CAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTC	9	0.22499999999999998	No Hit
GTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCCCGA	9	0.22499999999999998	No Hit
CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGC	9	0.22499999999999998	No Hit
AGAAATTTGAATGATGCGTCGCCAGCACAGAGGCCATGCGATCCGTCGAG	8	0.2	No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG	8	0.2	No Hit
ATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAA	8	0.2	No Hit
TTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCA	8	0.2	No Hit
CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGT	8	0.2	No Hit
CAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATTCCT	8	0.2	No Hit
CCCCTTTCGAAGTTCGCGCTTAAGCGCTATCAGACGGGTTTCCCCCGACT	8	0.2	No Hit
CCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAAGCGCCAT	8	0.2	No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC	8	0.2	No Hit
CTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCG	8	0.2	No Hit
CTTTATCACTGAGCGGTCATTTAGGGGCCTTAGCTGGTGATCCGGGCTGT	7	0.17500000000000002	No Hit
GGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAGTC	7	0.17500000000000002	No Hit
CAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCTCTGGGTT	7	0.17500000000000002	No Hit
CGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCC	7	0.17500000000000002	No Hit
CTGACAATGTCCTCCGCCCGGATCGACCCGCCGAAGCGAGCCTTGGGACC	7	0.17500000000000002	No Hit
CGCAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGGTTCCTCCG	7	0.17500000000000002	No Hit
CGGCCAGTGAGACGGTGGGGGATAAGCTTCATCGTCGAGAGGGAAACAGC	7	0.17500000000000002	No Hit
CACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAACT	7	0.17500000000000002	No Hit
AAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCA	7	0.17500000000000002	No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG	7	0.17500000000000002	No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT	7	0.17500000000000002	No Hit
AAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGA	6	0.15	No Hit
CTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGT	6	0.15	No Hit
CGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTT	6	0.15	No Hit
GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT	6	0.15	No Hit
CTCGCTTCGGCGGGTCGATCCGGGCGGAGGACATTGTCAGGTGGGGAGTT	6	0.15	No Hit
CTTTTATCTAATAAATGCGTCCCTTCCATAAGTCGGGGTTTGTTGCACGT	6	0.15	No Hit
GGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAGGGGC	6	0.15	No Hit
CAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACT	6	0.15	No Hit
CACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTAC	6	0.15	No Hit
GGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTT	6	0.15	No Hit
GGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGG	6	0.15	No Hit
CCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAACCCTTGGGTTTTC	6	0.15	No Hit
CGATACGCTCCTGGTCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTAC	6	0.15	No Hit
CGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAAA	6	0.15	No Hit
CGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCT	6	0.15	No Hit
CGCCCTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAG	6	0.15	No Hit
CGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAAT	6	0.15	No Hit
TGGGAGTATAGCATGGGTTACTTCAGCGCCGTAGCGCCTGGTACTCGAAC	6	0.15	No Hit
ATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTTA	6	0.15	No Hit
CGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAG	6	0.15	No Hit
AAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATG	5	0.125	No Hit
GAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAGTCT	5	0.125	No Hit
CCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCT	5	0.125	No Hit
TCCCCTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAG	5	0.125	No Hit
CGGAGATTGGCCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGAC	5	0.125	No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCT	5	0.125	No Hit
TTCCCCTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAA	5	0.125	No Hit
ATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTG	5	0.125	No Hit
CTTTAATGGGCGAACAGCCCAACCCTTGGAACATACTACAGCCCCAGGTG	5	0.125	No Hit
CTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC	5	0.125	No Hit
CGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGT	5	0.125	No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGA	5	0.125	No Hit
GTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGC	5	0.125	No Hit
CGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCACGGCCG	5	0.125	No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA	5	0.125	No Hit
CGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATT	5	0.125	No Hit
CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCATTTTGCCGACTTCC	5	0.125	No Hit
CTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCG	5	0.125	No Hit
CGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCG	5	0.125	No Hit
GTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTAACGCAAT	5	0.125	No Hit
CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG	5	0.125	No Hit
CTAATACGTGCAACAAACCCCGACTTATGGAAGGGACGCATTTATTAGAT	5	0.125	No Hit
AAAAAAGCGTCGTGCTGCTAGGCGAAGCGGTGGAGTGCCGCACCCTAGAT	5	0.125	No Hit
CTTCACTGTTCCCTGGGATTGGCTTTGGGCTTTTCCTGCGCAGCTTAGGT	5	0.125	No Hit
CCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCA	5	0.125	No Hit
GGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGG	5	0.125	No Hit
GTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCA	5	0.125	No Hit
CGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCA	5	0.125	No Hit
TGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAGGGG	5	0.125	No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC	5	0.125	No Hit
CCGACTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAAC	5	0.125	No Hit
ACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGAT	5	0.125	No Hit
CTGGGAACGGCTCTCTCGGGAGCTTTCCCCGGGCGTCGAACAGTCAGCTC	5	0.125	No Hit
CTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTT	5	0.125	No Hit
CCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA	5	0.125	No Hit
CTCCGCCCGGATCGACCCGCCGAAGCGAGCCTTGGGACCAAAAACAGGGG	5	0.125	No Hit
CTCCCCTACCGATGCATTTTGACATCCCACAGCTTCGGCAGATCGCTTAG	5	0.125	No Hit
TATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCAA	5	0.125	No Hit
GTCGAGTTATCATGAATCATCAGAGCAACGGGCAGAGCCCGCGTCGACCT	5	0.125	No Hit
CTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGTCCTACCTATT	5	0.125	No Hit
CTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCATAG	5	0.125	No Hit
CGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGCTCTCGATT	5	0.125	No Hit
ATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAAC	5	0.125	No Hit
TGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAACTCGTTG	5	0.125	No Hit
TAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTTAA	5	0.125	No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.025	0.0	0.0	0.0	0.0
80-81	0.037500000000000006	0.0	0.0	0.0	0.0
82-83	0.0625	0.0	0.0	0.0	0.0
84-85	0.0875	0.0	0.0	0.0	0.0
86-87	0.1	0.0	0.0	0.0	0.0
88-89	0.1	0.0	0.0	0.0	0.0
90-91	0.1125	0.0	0.0	0.0	0.0
92-93	0.125	0.0	0.0	0.0	0.0
94-95	0.175	0.0	0.0	0.0	0.0
96-97	0.2875	0.0	0.0	0.0	0.0
98-99	0.3	0.0	0.0	0.0	0.0
100-101	0.3375	0.0	0.0	0.0	0.0
102-103	0.3625	0.0	0.0	0.0	0.0
104-105	0.375	0.0	0.0	0.0	0.0
106-107	0.48750000000000004	0.0	0.0	0.0	0.0
108-109	0.6	0.0	0.0	0.0	0.0
110-111	0.675	0.0	0.0	0.0	0.0
112-113	0.7375	0.0	0.0	0.0	0.0
114-115	0.8	0.0	0.0	0.0	0.0
116-117	0.8625	0.0	0.0	0.0	0.0
118-119	0.925	0.0	0.0	0.0	0.0
120-121	1.0	0.0	0.0	0.0	0.0
122-123	1.025	0.0	0.0	0.0	0.0
124-125	1.15	0.0	0.0	0.0	0.0
126-127	1.2625	0.0	0.0	0.0	0.0
128-129	1.4	0.0	0.0	0.0	0.0
130-131	1.525	0.0	0.0	0.0	0.0
132-133	1.65	0.0	0.0	0.0	0.0
134-135	1.7875	0.0	0.0	0.0	0.0
136-137	1.9125	0.0	0.0	0.0	0.0
138	2.075	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGATTC	10	0.006973645	144.0	3
TTCCCTT	10	0.006973645	144.0	7
CCTCCTA	10	0.006973645	144.0	4
CAAGGAT	10	0.006973645	144.0	1
CCCTCCT	10	0.006973645	144.0	3
TCCCTTA	10	0.006973645	144.0	8
>>END_MODULE
Read 5963514 spots for SRR31913495.sra
Written 5963514 spots for SRR31913495.sra
Read 5963515 spots for SRR31913495.sra
Written 5963515 spots for SRR31913495.sra
Read 5963514 spots for SRR31913495.sra
Written 5963514 spots for SRR31913495.sra
Read 5963514 spots for SRR31913495.sra
Written 5963514 spots for SRR31913495.sra
SRR ids: ['SRR31913495.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_l8ht1yq9
SRR31913495.sra spots: 23854057
blocks: [[1, 5963514], [5963515, 11927028], [11927029, 17890542], [17890543, 23854057]]
SRR31913495 file size 8758924
SRR31913495 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 4 -o SRR31913495 SRR31913495_1.fastq SRR31913495_2.fastq
Input file:	SRR31913495_1.fastq
Paired file:	SRR31913495_2.fastq
trimmed:	SRR31913495-trimmed-pair1.fastq, SRR31913495-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	4
Wed Oct  1 12:43:30 2025 >> started

Wed Oct  1 12:44:34 2025 >> done (63.905s)
23854057 read pairs processed; of these:
      51 ( 0.00%) short read pairs filtered out after trimming by size control
      64 ( 0.00%) empty read pairs filtered out after trimming by size control
23853942 (100.00%) read pairs available; of these:
  967964 ( 4.06%) trimmed read pairs available after processing
22885978 (95.94%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       4	  0.00%
 19	       3	  0.00%
 20	       2	  0.00%
 21	       6	  0.00%
 22	       5	  0.00%
 23	      11	  0.00%
 24	       8	  0.00%
 25	       4	  0.00%
 26	       7	  0.00%
 27	      14	  0.00%
 28	       6	  0.00%
 29	      27	  0.00%
 30	      10	  0.00%
 31	      22	  0.00%
 32	      29	  0.00%
 33	      29	  0.00%
 34	      39	  0.00%
 35	      41	  0.00%
 36	      43	  0.00%
 37	      36	  0.00%
 38	      50	  0.00%
 39	      84	  0.00%
 40	     111	  0.00%
 41	     122	  0.00%
 42	     134	  0.00%
 43	     145	  0.00%
 44	     156	  0.00%
 45	     126	  0.00%
 46	     130	  0.00%
 47	     128	  0.00%
 48	     185	  0.00%
 49	     189	  0.00%
 50	     278	  0.00%
 51	     267	  0.00%
 52	     316	  0.00%
 53	     335	  0.00%
 54	     327	  0.00%
 55	     298	  0.00%
 56	     270	  0.00%
 57	     262	  0.00%
 58	     306	  0.00%
 59	     306	  0.00%
 60	     437	  0.00%
 61	     552	  0.00%
 62	     589	  0.00%
 63	     595	  0.00%
 64	     552	  0.00%
 65	     554	  0.00%
 66	     586	  0.00%
 67	     510	  0.00%
 68	     576	  0.00%
 69	     547	  0.00%
 70	     734	  0.00%
 71	     793	  0.00%
 72	     959	  0.00%
 73	    1018	  0.00%
 74	    1022	  0.00%
 75	    1017	  0.00%
 76	    1125	  0.00%
 77	    1102	  0.00%
 78	    1105	  0.00%
 79	    1198	  0.01%
 80	    1189	  0.00%
 81	    1369	  0.01%
 82	    1679	  0.01%
 83	    1800	  0.01%
 84	    1888	  0.01%
 85	    2141	  0.01%
 86	    1927	  0.01%
 87	    2118	  0.01%
 88	    1941	  0.01%
 89	    2114	  0.01%
 90	    2090	  0.01%
 91	    2766	  0.01%
 92	    2972	  0.01%
 93	    3188	  0.01%
 94	    3646	  0.02%
 95	    4386	  0.02%
 96	    4477	  0.02%
 97	    4099	  0.02%
 98	    4706	  0.02%
 99	    4390	  0.02%
100	    4536	  0.02%
101	    5558	  0.02%
102	    5391	  0.02%
103	    6043	  0.03%
104	    6359	  0.03%
105	    6667	  0.03%
106	    6697	  0.03%
107	    6790	  0.03%
108	    7496	  0.03%
109	    7296	  0.03%
110	    7796	  0.03%
111	    7757	  0.03%
112	    9274	  0.04%
113	    8691	  0.04%
114	    9085	  0.04%
115	   11323	  0.05%
116	   10682	  0.04%
117	   11094	  0.05%
118	   10818	  0.05%
119	   12249	  0.05%
120	   12165	  0.05%
121	   12808	  0.05%
122	   14608	  0.06%
123	   13196	  0.06%
124	   14017	  0.06%
125	   15150	  0.06%
126	   15918	  0.07%
127	   16344	  0.07%
128	   17606	  0.07%
129	   17665	  0.07%
130	   18503	  0.08%
131	   19955	  0.08%
132	   19720	  0.08%
133	   23424	  0.10%
134	   23919	  0.10%
135	   26002	  0.11%
136	   27916	  0.12%
137	   29466	  0.12%
138	   28471	  0.12%
139	   28355	  0.12%
140	   26817	  0.11%
141	   28570	  0.12%
142	   30707	  0.13%
143	   28565	  0.12%
144	   33794	  0.14%
145	   36638	  0.15%
146	   34363	  0.14%
147	   37033	  0.16%
148	   41081	  0.17%
149	   38258	  0.16%
150	22885978	 95.94%
23853942 reads passed initial QC


criterion=sequence-density
sequence-density=4.69
sequence-density-rank=1
fanout-score=3.40
fanout-score-rank=23
prefix-density=4.70
prefix-fanout=3.4
sequence=GCGCTTAAGCGC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=37
fanout-score=92.51
fanout-score-rank=1
prefix-density=0.55
prefix-fanout=1.0
sequence=GGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCACGGCCGGCTTCTTAGAGGGACTATGGCCGTTTAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTCACACCTTGGCCGACAGGCCCGGGTAATCTTTGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGATCCGGTGAAGTGTTCGGATCGCGGCGACGTGGGTGGTTCGCCGC


criterion=sequence-density
sequence-density=4.62
sequence-density-rank=1
fanout-score=3.32
fanout-score-rank=21
prefix-density=4.65
prefix-fanout=3.3
sequence=GCGCTTAAGCGC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=36
fanout-score=91.60
fanout-score-rank=1
prefix-density=0.54
prefix-fanout=1.0
sequence=GGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCACGGCCGGCTTCTTAGAGGGACTATGGCCGTTTAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTCACACCTTGGCCGACAGGCCCGGGTAATCTTTGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGATCCGGTGAAGTGTTCGGATCGCGGCGACGTGGGTGGTTCGCCGC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 4 -x GCGCTTAAGCGC -y GCGCTTAAGCGC -o SRR31913495 SRR31913495_1.fastq SRR31913495_2.fastq
Input file:	SRR31913495_1.fastq
Paired file:	SRR31913495_2.fastq
trimmed:	SRR31913495-trimmed-pair1.fastq, SRR31913495-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GCGCTTAAGCGC
-- paired 3' end adapter sequence (-y):	GCGCTTAAGCGC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	4
Wed Oct  1 12:45:51 2025 >> started

Wed Oct  1 12:46:15 2025 >> done (24.168s)
14312365 read pairs processed; of these:
    2533 ( 0.02%) short read pairs filtered out after trimming by size control
     138 ( 0.00%) empty read pairs filtered out after trimming by size control
14309694 (99.98%) read pairs available; of these:
       5 ( 0.00%) trimmed read pairs available after processing
14309689 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       2	  0.00%
 19	       3	  0.00%
 20	       2	  0.00%
 21	       4	  0.00%
 22	       4	  0.00%
 23	       6	  0.00%
 24	       4	  0.00%
 25	       2	  0.00%
 26	       3	  0.00%
 27	       8	  0.00%
 28	       3	  0.00%
 29	      13	  0.00%
 30	       8	  0.00%
 31	      13	  0.00%
 32	      19	  0.00%
 33	      16	  0.00%
 34	      21	  0.00%
 35	      26	  0.00%
 36	      21	  0.00%
 37	      24	  0.00%
 38	      21	  0.00%
 39	      47	  0.00%
 40	      64	  0.00%
 41	      79	  0.00%
 42	      87	  0.00%
 43	      90	  0.00%
 44	     107	  0.00%
 45	      75	  0.00%
 46	      80	  0.00%
 47	      80	  0.00%
 48	     110	  0.00%
 49	     127	  0.00%
 50	     179	  0.00%
 51	     159	  0.00%
 52	     202	  0.00%
 53	     203	  0.00%
 54	     191	  0.00%
 55	     176	  0.00%
 56	     155	  0.00%
 57	     166	  0.00%
 58	     182	  0.00%
 59	     199	  0.00%
 60	     256	  0.00%
 61	     311	  0.00%
 62	     348	  0.00%
 63	     373	  0.00%
 64	     337	  0.00%
 65	     341	  0.00%
 66	     347	  0.00%
 67	     317	  0.00%
 68	     347	  0.00%
 69	     316	  0.00%
 70	     443	  0.00%
 71	     466	  0.00%
 72	     597	  0.00%
 73	     615	  0.00%
 74	     613	  0.00%
 75	     606	  0.00%
 76	     671	  0.00%
 77	     658	  0.00%
 78	     668	  0.00%
 79	     695	  0.00%
 80	     699	  0.00%
 81	     819	  0.01%
 82	     997	  0.01%
 83	    1068	  0.01%
 84	    1120	  0.01%
 85	    1298	  0.01%
 86	    1136	  0.01%
 87	    1301	  0.01%
 88	    1185	  0.01%
 89	    1294	  0.01%
 90	    1246	  0.01%
 91	    1655	  0.01%
 92	    1738	  0.01%
 93	    1866	  0.01%
 94	    2238	  0.02%
 95	    2621	  0.02%
 96	    2687	  0.02%
 97	    2434	  0.02%
 98	    2780	  0.02%
 99	    2665	  0.02%
100	    2735	  0.02%
101	    3339	  0.02%
102	    3219	  0.02%
103	    3588	  0.03%
104	    3818	  0.03%
105	    3982	  0.03%
106	    3966	  0.03%
107	    4081	  0.03%
108	    4539	  0.03%
109	    4449	  0.03%
110	    4599	  0.03%
111	    4649	  0.03%
112	    5525	  0.04%
113	    5246	  0.04%
114	    5373	  0.04%
115	    6781	  0.05%
116	    6415	  0.04%
117	    6636	  0.05%
118	    6408	  0.04%
119	    7307	  0.05%
120	    7328	  0.05%
121	    7700	  0.05%
122	    8740	  0.06%
123	    7899	  0.06%
124	    8369	  0.06%
125	    9057	  0.06%
126	    9674	  0.07%
127	    9799	  0.07%
128	   10512	  0.07%
129	   10689	  0.07%
130	   10974	  0.08%
131	   11967	  0.08%
132	   11816	  0.08%
133	   14113	  0.10%
134	   14338	  0.10%
135	   15515	  0.11%
136	   16632	  0.12%
137	   17724	  0.12%
138	   17015	  0.12%
139	   16955	  0.12%
140	   15981	  0.11%
141	   17014	  0.12%
142	   18312	  0.13%
143	   17171	  0.12%
144	   20101	  0.14%
145	   22020	  0.15%
146	   20657	  0.14%
147	   22231	  0.16%
148	   24469	  0.17%
149	   22963	  0.16%
150	13730151	 95.95%


criterion=sequence-density
sequence-density=4.68
sequence-density-rank=1
fanout-score=3.39
fanout-score-rank=22
prefix-density=4.71
prefix-fanout=3.4
sequence=GCGCTTAAGCGC


criterion=fanout-score
sequence-density=0.60
sequence-density-rank=22
fanout-score=18.45
fanout-score-rank=1
prefix-density=4.70
prefix-fanout=2.4
sequence=GCTTAAGCGCGAACTTCGAAAGGGGATCCGGTTAAAATTCCGGAACCGGGACGTGGCGGTTGACGGCAACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCTGGAAACGGC


criterion=sequence-density
sequence-density=4.61
sequence-density-rank=1
fanout-score=3.30
fanout-score-rank=21
prefix-density=4.64
prefix-fanout=3.3
sequence=GCGCTTAAGCGC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=36
fanout-score=94.18
fanout-score-rank=1
prefix-density=0.54
prefix-fanout=1.0
sequence=GGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCACGGCCGGCTTCTTAGAGGGACTATGGCCGTTTAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTCACACCTTGGCCGACAGGCCCGGGTAATCTTTGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGATCCGGTGAAGTGTTCGGATCGCGGCGACGTGGGTGGTTCGCCGC
SRR31913495 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Oct 01 12:46:58
                             Started mapping on |	Oct 01 12:46:58
                                    Finished on |	Oct 01 12:51:22
       Mapping speed, Million of reads per hour |	325.24

                          Number of input reads |	23851271
                      Average input read length |	298
                                    UNIQUE READS:
                   Uniquely mapped reads number |	5849553
                        Uniquely mapped reads % |	24.53%
                          Average mapped length |	296.77
                       Number of splices: Total |	158898
            Number of splices: Annotated (sjdb) |	142351
                       Number of splices: GT/AG |	144147
                       Number of splices: GC/AG |	1457
                       Number of splices: AT/AC |	1103
               Number of splices: Non-canonical |	12191
                      Mismatch rate per base, % |	0.48%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.18
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	17542865
             % of reads mapped to multiple loci |	73.55%
        Number of reads mapped to too many loci |	284
             % of reads mapped to too many loci |	0.00%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.92%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	458853	458853	458853
N_multimapping	17542865	17542865	17542865
N_noFeature	4241119	5042690	5038818
N_ambiguous	18261	4670	4627
UnstrandedReadsAssigned:1590173 PositiveStrandReadsAssigned:802193 NegativeStrandReadsAssigned:806108
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR31913495 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 48,359
[index] number of k-mers: 45,567,218
[index] number of equivalence classes: 93,973
[quant] running in paired-end mode
[quant] will process pair 1: SRR31913495-trimmed-pair1.fastq
                             SRR31913495-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 23,851,271 reads, 868,897 reads pseudoaligned
[quant] estimated average fragment length: 203.721
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 883 rounds

  48360 SRR31913495.ke.tsv
  32833 SRR31913495.se.tsv
  81193 total
==> SRR31913495.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AT1G19090.1	2509	2306.28	0	0
AT1G18320.1	699	496.279	0	0
AT5G11100.1	1709	1506.28	0	0
AT4G35335.1	1280	1077.28	2	0.311439
AT1G60930.1	3876	3673.28	1	0.0456686
AT1G17000.1	2351	2148.28	0	0
AT3G55270.1	3130	2927.28	27	1.54729
AT4G16150.1	3181	2978.28	13	0.732233
AT3G18150.1	1370	1167.28	0	0

==> SRR31913495.se.tsv <==
AT1G01010	3
AT1G01020	6
AT1G03987	0
AT1G01030	1
AT1G01040	10
AT1G03993	0
AT1G01046	0
AT1G01050	3
AT1G03997	0
AT1G01060	0
SRR31913495 completed mapping pipeline successfully
