Starting /dee2/code/volunteer_pipeline.sh SRR9060564
    current disk space = 832372660
    free memory = 113744188 
SRR9060564 SRAfilesize
84f369f198e74874535b47be0155af3c  SRR9060564.sra
SRR9060564.sra file validated
SRR9060564 is single end
SRR9060564 is conventional basespace
SRR9060564 read1 length is 48-51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR9060564_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	48-51
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.98325	32.0	32.0	32.0	21.0	32.0
2	30.84875	32.0	32.0	32.0	32.0	32.0
3	31.57175	32.0	32.0	32.0	32.0	32.0
4	31.609	32.0	32.0	32.0	32.0	32.0
5	31.50275	32.0	32.0	32.0	32.0	32.0
6	33.21625	36.0	36.0	36.0	32.0	36.0
7	35.00825	36.0	36.0	36.0	36.0	36.0
8	34.96975	36.0	36.0	36.0	36.0	36.0
9	35.06875	36.0	36.0	36.0	36.0	36.0
10	34.97575	36.0	36.0	36.0	36.0	36.0
11	35.08475	36.0	36.0	36.0	36.0	36.0
12	35.06575	36.0	36.0	36.0	36.0	36.0
13	35.12575	36.0	36.0	36.0	36.0	36.0
14	35.09875	36.0	36.0	36.0	36.0	36.0
15	35.08875	36.0	36.0	36.0	36.0	36.0
16	35.30225	36.0	36.0	36.0	36.0	36.0
17	35.10875	36.0	36.0	36.0	36.0	36.0
18	35.02475	36.0	36.0	36.0	36.0	36.0
19	34.87375	36.0	36.0	36.0	36.0	36.0
20	35.15	36.0	36.0	36.0	36.0	36.0
21	34.988	36.0	36.0	36.0	36.0	36.0
22	35.003	36.0	36.0	36.0	36.0	36.0
23	35.027	36.0	36.0	36.0	36.0	36.0
24	34.843	36.0	36.0	36.0	36.0	36.0
25	34.971	36.0	36.0	36.0	36.0	36.0
26	35.02425	36.0	36.0	36.0	36.0	36.0
27	34.89475	36.0	36.0	36.0	36.0	36.0
28	35.079	36.0	36.0	36.0	36.0	36.0
29	34.929	36.0	36.0	36.0	36.0	36.0
30	35.03725	36.0	36.0	36.0	36.0	36.0
31	34.98175	36.0	36.0	36.0	36.0	36.0
32	34.89725	36.0	36.0	36.0	36.0	36.0
33	34.90375	36.0	36.0	36.0	36.0	36.0
34	34.70325	36.0	36.0	36.0	32.0	36.0
35	34.98075	36.0	36.0	36.0	36.0	36.0
36	34.726	36.0	36.0	36.0	36.0	36.0
37	34.63025	36.0	36.0	36.0	32.0	36.0
38	34.88575	36.0	36.0	36.0	36.0	36.0
39	34.71025	36.0	36.0	36.0	36.0	36.0
40	34.848	36.0	36.0	36.0	36.0	36.0
41	34.80925	36.0	36.0	36.0	36.0	36.0
42	34.91325	36.0	36.0	36.0	36.0	36.0
43	34.97475	36.0	36.0	36.0	36.0	36.0
44	35.04475	36.0	36.0	36.0	36.0	36.0
45	35.047	36.0	36.0	36.0	36.0	36.0
46	34.85575	36.0	36.0	36.0	36.0	36.0
47	34.905	36.0	36.0	36.0	36.0	36.0
48	34.774	36.0	36.0	36.0	36.0	36.0
49	34.71792948237059	36.0	36.0	36.0	36.0	36.0
50	34.73879989139289	36.0	36.0	36.0	36.0	36.0
51	34.47229972100438	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	6.0
23	11.0
24	21.0
25	33.0
26	28.0
27	34.0
28	52.0
29	53.0
30	65.0
31	97.0
32	107.0
33	185.0
34	584.0
35	2723.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.025000000000002	53.425	7.324999999999999	16.225
2	29.549999999999997	33.5	24.2	12.75
3	30.95	17.150000000000002	30.7	21.2
4	37.425000000000004	18.65	20.75	23.175
5	39.475	24.224999999999998	16.875	19.425
6	29.751191106405507	25.727898358920065	29.77766013763896	14.743250397035467
7	25.6	24.474999999999998	27.875	22.05
8	31.825	27.85	20.925	19.400000000000002
9	26.8	21.65	25.8	25.75
10	31.974999999999998	21.224999999999998	31.574999999999996	15.225
11	33.1	27.675	24.325	14.899999999999999
12	25.874999999999996	25.374999999999996	27.224999999999998	21.525
13	27.575	26.075	25.724999999999998	20.625
14	28.625	19.55	27.975	23.849999999999998
15	26.424999999999997	25.124999999999996	26.825	21.625
16	33.425	23.175	19.575	23.825
17	36.65	18.175	27.6	17.575
18	27.025	26.700000000000003	25.724999999999998	20.549999999999997
19	26.0	28.4	24.975	20.625
20	30.825000000000003	21.375	27.150000000000002	20.65
21	30.7	25.074999999999996	23.575	20.65
22	27.375	30.3	23.5	18.825
23	30.175	27.700000000000003	27.750000000000004	14.374999999999998
24	23.275000000000002	28.9	26.450000000000003	21.375
25	22.825	33.4	26.1	17.675
26	31.125000000000004	23.599999999999998	23.724999999999998	21.55
27	22.075	25.1	29.575000000000003	23.25
28	32.7	19.875	27.925	19.5
29	35.5	19.05	23.25	22.2
30	34.2	23.825	26.974999999999998	15.0
31	30.625000000000004	30.599999999999998	27.025	11.75
32	24.575	28.075	35.8	11.55
33	25.924999999999997	24.425	35.15	14.499999999999998
34	23.225	23.5	31.0	22.275
35	24.725	18.325	35.3	21.65
36	22.55	20.45	34.699999999999996	22.3
37	31.674999999999997	19.6	25.25	23.474999999999998
38	36.775000000000006	17.2	27.700000000000003	18.325
39	36.375	20.275000000000002	24.825	18.525
40	33.85	19.85	27.125	19.175
41	32.25	15.1	33.324999999999996	19.325
42	31.4	14.499999999999998	26.1	28.000000000000004
43	29.275000000000002	13.8	23.549999999999997	33.375
44	30.475	17.675	19.85	32.0
45	29.5	24.6	17.375	28.525
46	35.975	24.0	14.85	25.174999999999997
47	44.05	22.15	13.825000000000001	19.975
48	36.449999999999996	26.775	11.85	24.925
49	31.757939484871216	31.50787696924231	14.278569642410602	22.455613903475868
50	28.753733369535706	27.233233776812384	13.657344556068423	30.355688297583495
51	24.192905540055797	0.0	24.033479473893983	51.77361498605022
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	1.5
18	2.0
19	3.0
20	4.0
21	6.0
22	8.0
23	11.0
24	14.0
25	10.5
26	10.0
27	14.5
28	15.0
29	12.5
30	11.0
31	11.0
32	11.0
33	10.5
34	10.5
35	11.0
36	15.5
37	18.0
38	13.5
39	13.0
40	32.0
41	57.5
42	97.5
43	132.5
44	212.5
45	307.0
46	334.0
47	370.0
48	445.0
49	483.5
50	476.5
51	499.0
52	553.5
53	642.5
54	644.0
55	560.0
56	502.5
57	464.0
58	381.0
59	252.5
60	148.0
61	81.0
62	41.5
63	19.0
64	10.5
65	4.5
66	2.5
67	1.0
68	0.5
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	5.55
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
48	1.0
49	316.0
50	1174.0
51	2509.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.449999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.38699690402477	36.525
2	10.68111455108359	10.35
3	4.024767801857585	5.8500000000000005
4	2.683178534571723	5.2
5	1.6511867905056758	4.0
6	1.0319917440660475	3.0
7	0.46439628482972134	1.575
8	0.5675954592363261	2.1999999999999997
9	0.46439628482972134	2.025
>10	2.8379772961816303	23.549999999999997
>50	0.20639834881320948	5.7250000000000005
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGGAATCCGACTGTCTAATTAAAACTGGAATTCTCGGGTGCCAAGGAACT	69	1.725	RNA PCR Primer, Index 1 (100% over 25bp)
GATTGTGCTTGCTTGCGGACGCTTTCTGGTGTTGGAATTCTCGGGTGCCA	56	1.4000000000000001	No Hit
AACGAGCGAGACTCTAGCCTGCTAAATTGGAATTCTCGGGTGCCAAGGAAC	53	1.325	RNA PCR Primer, Index 1 (100% over 24bp)
AGTAACGGCGAGTGAAACGGGAAGAGCCCTGGAATTCTCGGGTGCCAAGG	51	1.275	Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGGTTTGAACCGGGTAAAAGTCGTAACTGGAATTCTCGGGTGCCAAGG	41	1.0250000000000001	Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCAGAACTGGCGCTGTGGGATGAACCTGGAATTCTCGGGTGCCAAGGA	38	0.95	RNA PCR Primer, Index 1 (100% over 22bp)
AAGCGGTGTTTAGCCGCACGAGATTGAGCGATAACTGGAATTCTCGGGTGC	38	0.95	No Hit
AAGCGGTGTTTAGCCGCACGAGATTGAGCGATTGGAATTCTCGGGTGCCA	37	0.9249999999999999	No Hit
AACGGCGAGTGAAACGGGAAGAGCCCTGGAATTCTCGGGTGCCAAGGAACT	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 25bp)
GAGCAGAACTGGCGCTGTGGGATGAACCTGGAATTCTCGGGTGCCAAGGA	31	0.775	RNA PCR Primer, Index 1 (100% over 22bp)
ACGGGAAAACTCACCCGGTCCGGACACCATTTGGAATTCTCGGGTGCCA	31	0.775	No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTTGGAATTCTCGGGTG	26	0.65	No Hit
AAACGACCCGAAAGATGGTGAACTATGCCTGAGCTGGAATTCTCGGGTGCC	26	0.65	No Hit
AGCTCACGTTGATTACGTCCCTGCCCTTTGTACACTGGAATTCTCGGGTGC	21	0.525	No Hit
ATTGTGAAGAGTCATGAGAGGTGTAGCTGGAATTCTCGGGTGCCAAGGAAC	21	0.525	RNA PCR Primer, Index 1 (100% over 24bp)
GAGCGGTGTTTAGCCGCACGAGATTGAGCGATAACTGGAATTCTCGGGTGC	21	0.525	No Hit
AGATGGTAGTTTATTGGACTACCATGGTTGTTACGGGTTGGAATTCTCGGG	21	0.525	No Hit
AATGTAGGTAAGGGAAGTCGGCAAACTTGGAATTCTCGGGTGCCAAGGAAC	20	0.5	RNA PCR Primer, Index 1 (100% over 24bp)
CGTGCTCTTTGAACGGCCCTTAAAACACCTGGAATTCTCGGGTGCCAAGG	20	0.5	Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTGC	19	0.475	No Hit
GAACGACCCGAAAGATGGTGAACTATGCCTGAGCTGGAATTCTCGGGTGCC	18	0.44999999999999996	No Hit
CTGTGGTGTCTCGTGCTCTTTGAACGGCCCTTAAAACTGGAATTCTCGGGT	17	0.42500000000000004	No Hit
CAGGGAATCCGACTGTCTAATTAAAACTGGAATTCTCGGGTGCCAAGGAAC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 24bp)
CTCGTGCTCTTTGAACGGCCCTTAAAACTGGAATTCTCGGGTGCCAAGGA	16	0.4	RNA PCR Primer, Index 1 (100% over 22bp)
AGAACTGATTTAATGGTTAAGAGGGACTGGAATTCTCGGGTGCCAAGGAAC	16	0.4	RNA PCR Primer, Index 1 (100% over 24bp)
CTCGTGCTCTTTGAACGGCCCTTAAAACACCTGGAATTCTCGGGTGCCA	15	0.375	No Hit
ACCCTACTGTTGACTTGTTATTGCGAAAGTTGGAATTCTCGGGTGCCAAGG	15	0.375	Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGCC	15	0.375	No Hit
AAGGAACGGACTCCCTGGAATTGGTTCTGGAATTCTCGGGTGCCAAGGAAC	15	0.375	RNA PCR Primer, Index 1 (100% over 24bp)
TACCTGATTGATTCTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 33bp)
ATATCATTTAGCGGAGGAAAAGAAACTTGGAATTCTCGGGTGCCAAGGAAC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 24bp)
ATTGCGAAGCAGAATTCGCCAAGCGTTGGATTGTTCTGGAATTCTCGGGTG	14	0.35000000000000003	No Hit
AACCGTACTGGAACCGGTACGGACTCAGGGAATCCGACTGTCTTGGAATTC	14	0.35000000000000003	No Hit
AGCACCAACTGGGCCTCCAGTTGGTACGTCTGGTTCTGGAATTCTCGGGTG	14	0.35000000000000003	No Hit
CGTGCTCTTTGAACGGCCCTTAAAACTGGAATTCTCGGGTGCCAAGGAACT	13	0.325	RNA PCR Primer, Index 1 (100% over 25bp)
AGAACTGGCGCTGTGGGATGAACCTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
AAAGGGTGTTAAAACCTTGCGGCGAAATGAAAGTTGGAATTCTCGGGTGCC	13	0.325	No Hit
AGCTCACGTTGATTACGTCCCTGCCCTTTGTACTGGAATTCTCGGGTGCC	13	0.325	No Hit
AAAGGTCAGTCTCGAATTGGCCGACGTGGGATTGGAATTCTCGGGTGCCA	13	0.325	No Hit
ATTGGTTTGAACCGGGTAAAAGTCGTAACAAGGTTGGAATTCTCGGGTGCC	13	0.325	No Hit
AAGAGAAGGTGTCTTGCTGGCGGTAGTGGAATTCTCGGGTGCCAAGGAACT	12	0.3	RNA PCR Primer, Index 1 (100% over 25bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTTTGGAATTCTCGGGT	12	0.3	No Hit
CGTGATTACCCGCTGAACTTAAGCATTGGAATTCTCGGGTGCCAAGGAACT	12	0.3	RNA PCR Primer, Index 1 (100% over 25bp)
AGAATAATGTAGGTAAGGGAAGTCGGCAAACTTGGAATTCTCGGGTGCCA	12	0.3	No Hit
AATGGTTAAGAGGGACAAACCGGGGGCATTCGTTGGAATTCTCGGGTGCC	12	0.3	No Hit
ATTTAGCGGAGGAAAAGAAACTTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
GAGCGGTGTTTAGCCGCACGAGATTGAGCGATTGGAATTCTCGGGTGCCA	11	0.27499999999999997	No Hit
GGGGAATCCGACTGTCTAATTAAAACTGGAATTCTCGGGTGCCAAGGAACT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 25bp)
AAAGAAATTGACGGAAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 31bp)
TTGTGATCGTTGCCGGGTGTCGTTTCCTTGGAATTCTCGGGTGCCAAGGA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 22bp)
AGTATCCTGATCGCTCATCTAAACTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
GCGCGAGAGGTGAAATTCGTGGACCGTTGGAATTCTCGGGTGCCAAGGAAC	10	0.25	RNA PCR Primer, Index 1 (100% over 24bp)
GTGCGTGTCACTTGTGACTGTGCAGAGGTTGAGCTGGAATTCTCGGGTGCC	10	0.25	No Hit
GGAACTGGCGCTGTGGGATGAACCTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
ACGGACTCAGGGAATCCGACTGTCTAATTAAAACTGGAATTCTCGGGTGCC	10	0.25	No Hit
ATCCTGATCGCTCATCTAAACTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
GGCACCAACTGGGCCTCCAGTTGGTACGTCTGGTTCTGGAATTCTCGGGTG	10	0.25	No Hit
AGCGGCACTGTTGGTTCGCTTGCTCCTTGGAGTTGGAATTCTCGGGTGCC	10	0.25	No Hit
CCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCC	10	0.25	No Hit
ATCATTTAGCGGAGGAAAAGAAACTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
GTTCCGATAACGAGCGAGACTCTAGCCTGCTAAATTGGAATTCTCGGGTGC	9	0.22499999999999998	No Hit
GCGTGTCACTTGTGACTGTGCAGAGGTTGAGCTGGAATTCTCGGGTGCCA	9	0.22499999999999998	No Hit
GACGGCGAGTGAAACGGGAAGAGCCCTGGAATTCTCGGGTGCCAAGGAACT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 25bp)
ATACTGCGATCTGTTGAGACTATCCTTTGATTGAGTGGAATTCTCGGGTGC	9	0.22499999999999998	No Hit
AATGGTTAAGAGGGACAAACCGGGGGCTGGAATTCTCGGGTGCCAAGGAAC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 24bp)
ATGAGATAGTGACTAAAAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 31bp)
AGCGCCAGGTCGTAACGAGTAGGAAGGTTGGAATTCTCGGGTGCCAAGGA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 22bp)
ATTGTGAAGAGTCATGAGAGGTGTAGCATTGGAATTCTCGGGTGCCAAGG	9	0.22499999999999998	Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCATATCATTTAGCGGAGGAAAAGAAACTTGGAATTCTCGGGTGCCA	8	0.2	No Hit
AGCAGGACGGTGGCCATGGAAGTCGGTTGGAATTCTCGGGTGCCAAGGAAC	8	0.2	RNA PCR Primer, Index 1 (100% over 24bp)
AATGTCGGGGCGCTAAATCACTTGCATTGGAATTCTCGGGTGCCAAGGAAC	8	0.2	RNA PCR Primer, Index 1 (100% over 24bp)
AGACTTGGGTATAGGGGCGAAAGACTTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
AGTTGTGATCGTTGCCGGGTGTCGTTTCCTTGGAATTCTCGGGTGCCAAGG	8	0.2	Illumina Small RNA Adapter 2 (100% over 21bp)
AGATGTCCGGGGCTGCACGCGTGCTACTGGAATTCTCGGGTGCCAAGGAAC	8	0.2	RNA PCR Primer, Index 1 (100% over 24bp)
TTGCGGACGCTTTCTGGTGTGTGCTTGGACCTCTGGAATTCTCGGGTGCC	8	0.2	No Hit
ATCCGGGACTGAACTGATTCGAGAAGAGTTGGAATTCTCGGGTGCCAAGG	8	0.2	Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGATTACCCGCTGAACTTAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CGTGATTACCCGCTGAACTTAAGCATATCTGGAATTCTCGGGTGCCAAGG	8	0.2	Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTGGATCGGAGACGGCCTGGGAATCTGGAATTCTCGGGTGCCAAGGAAC	8	0.2	RNA PCR Primer, Index 1 (100% over 24bp)
GGTAACGGCGAGTGAAACGGGAAGAGCCCTGGAATTCTCGGGTGCCAAGG	7	0.17500000000000002	Illumina Small RNA Adapter 2 (100% over 21bp)
GATTGNGCTTGCTTGCGGACGCTTTCTGGTGTTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
AAAGCTGAAACTTAAAGAAATTGACGGAAGGGCTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
GGTTCTAGTATCCTGATCGCTCATCTAAACTGGAATTCTCGGGTGCCAAGG	7	0.17500000000000002	Illumina Small RNA Adapter 2 (100% over 21bp)
AGATGGTAGTTTATTGGACTACCATGGTTGTTACGGGTAACTGGAATTCTC	7	0.17500000000000002	No Hit
AGGTAAGGGAAGTCGGCAAACTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
ATTCCGATAACGAGCGAGACTCTAGCCTGCTAAATTGGAATTCTCGGGTGC	7	0.17500000000000002	No Hit
CGAGAAGAGTGGGGACTGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
ATGAGACCCCATAAAAGGTGTTGGTTGATATTGACTGGAATTCTCGGGTGC	7	0.17500000000000002	No Hit
AGTGATTGTGCTTGCTTGCGGACGCTTTCTGGTGTTGGAATTCTCGGGTGC	6	0.15	No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGACTGGAATTCTCG	6	0.15	No Hit
GTTGCGAAGCAGAATTCGCCAAGCGTTGGATTGTTCTGGAATTCTCGGGTG	6	0.15	No Hit
GTTGGTTTGAACCGGGTAAAAGTCGTAACTGGAATTCTCGGGTGCCAAGG	6	0.15	Illumina Small RNA Adapter 2 (100% over 21bp)
AGTAGTTGGATGTGGGTCAGTCGATCCTAAGGTTGGAATTCTCGGGTGCC	6	0.15	No Hit
AGTTAAGGAACGGACTCCCTGGAATTGGTTCTGGAATTCTCGGGTGCCA	6	0.15	No Hit
CGGGGTGCAATTATTAGAACAGACCAAACTGGAATTCTCGGGTGCCAAGG	6	0.15	Illumina Small RNA Adapter 2 (100% over 21bp)
TACCTGATTGATTCTGTCAGCGCGATATGCTCTGGAATTCTCGGGTGCCA	6	0.15	No Hit
AGCGGAGGAAAAGAAACTAAAAAGGATTCCCTTAGTTGGAATTCTCGGGTG	6	0.15	No Hit
TTGCGGACGCTTTCTGGTGTGTGCTTGGACCTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
AGTTGGCAAACGACCCGAAAGATGGTGAACTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
TCTCAACCTGAACTCAGTCGTGATTACCCGCTGAACTTTGGAATTCTCGGG	6	0.15	No Hit
GAGCANAACTGGCGCTGTGGGATGAACCTGGAATTCTCGGGTGCCAAGGA	6	0.15	RNA PCR Primer, Index 1 (100% over 22bp)
AAGAGGGACAAACCGGGGGCATTCGTTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TCTCAACCTGAACTCAGTCGTGATTACCCGCTGGAATTCTCGGGTGCCA	6	0.15	No Hit
GGTCGTGATTACCCGCTGAACTTAAGCTGGAATTCTCGGGTGCCAAGGAAC	6	0.15	RNA PCR Primer, Index 1 (100% over 24bp)
AAGAGGTGTCAGAAAAGTTACCACAGGGATTGGAATTCTCGGGTGCCAAGG	6	0.15	Illumina Small RNA Adapter 2 (100% over 21bp)
GTGTCACTTGTGACTGTGCAGAGGTTGAGCTGGAATTCTCGGGTGCCAAGG	6	0.15	Illumina Small RNA Adapter 2 (100% over 21bp)
CGCTATCCGGGACTGAACTGATTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ATGCTACGCCGACGGCGTTGGCTGCTCGTTCTAGCCCGACTGGAATTCTCG	6	0.15	No Hit
GGCCAGTTGGTTGATGCTTGTCCGGCGCAGTTCTGGAATTCTCGGGTGCC	5	0.125	No Hit
GATGTAGGTAAGGGAAGTCGGCAAACTTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
AAGAGAACGTGAAATCGCTGGAGTGGAACCGGAGACTGGAATTCTCGGGTG	5	0.125	No Hit
AGTTCTGACCGTAAACGATGCCATCTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
AACGCCAGATCACTCTGGTTCAATGTCGGGGCGCTTGGAATTCTCGGGTGC	5	0.125	No Hit
AGACTTGGGTATAGGGGCGAAAGACTAATTGGAATTCTCGGGTGCCAAGG	5	0.125	Illumina Small RNA Adapter 2 (100% over 21bp)
ATTTGAGAGATTATAACTGAACGCCTCTAAGTTAGAATTGGAATTCTCGGG	5	0.125	No Hit
GTGCTTGGACCTCGGTTCTAGTATCCTGATCGCTCTGGAATTCTCGGGTGC	5	0.125	No Hit
CAGTCGTGATTACCCGCTGAACTTAAGCTGGAATTCTCGGGTGCCAAGGA	5	0.125	RNA PCR Primer, Index 1 (100% over 22bp)
AGGGANTCCGACTGTCTAATTAAAACTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
ATGCTACGCCGACGGCGTTGGCTGCTCGTTCTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
ATTTTTATCGCATTGGTTTGAACCGGGTTGGAATTCTCGGGTGCCAAGGA	5	0.125	RNA PCR Primer, Index 1 (100% over 22bp)
AATTGTTGACGCGCATGAATGGATTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
GACGAGCGAGACTCTAGCCTGCTAAATTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
AAATCGATCGATAGACTTGGGTATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACGGGAAAACTCACCCGGTCCGGACACCTGGAATTCTCGGGTGCCAAGGA	5	0.125	RNA PCR Primer, Index 1 (100% over 22bp)
ATCGGTAAACCGTTGAAATTCTTCCATGTCCGGGATTGGAATTCTCGGGTG	5	0.125	No Hit
AAGGAACGGACTCCCTGGAATTGGTTCAGCCAGAGATTGGAATTCTCGGGT	5	0.125	No Hit
ATGCCGAAAGGTATCGGTAAACCGTTGAAATTCTTCCTGGAATTCTCGGGT	5	0.125	No Hit
ACTGTTGACTTGTTATTGCGAAAGTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
ATCGCATTGGTTTGAACCGGGTAAAAGTCGTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
CGAGAAGAGTGGGGACTGTCGCTTCGAGGTTTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
ATAGCACCAACTGGGCCTCCAGTTGGTACGTCTGGTTCTGGAATTCTCGGG	5	0.125	No Hit
ACTTGTGACTGTGCAGAGGTTGAGCTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
ATGGAAGTCGGTATCCGCTAAGGAGTGTGTAACTGGAATTCTCGGGTGCC	5	0.125	No Hit
AAATGATTCTTCGAGTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG	5	0.125	Illumina Small RNA Adapter 2 (100% over 21bp)
CCGAAAGATGGTGAACTATGCCTGAGCTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
GGCAGGACGGTGGCCATGGAAGTCGGTTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
AGTTTGACATTGTGAAGAGTCATGAGAGGTGTAGCTGGAATTCTCGGGTGC	5	0.125	No Hit
AAATCGATCGATAGACTTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ATTGGTTTGAACCGGGTAAAAGTCGTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.1	0.0	0.0	0.0
2	0.0	0.125	0.0	0.0	0.0
3	0.0	0.125	0.0	0.0	0.0
4	0.0	0.15	0.0	0.0	0.0
5	0.0	0.175	0.0	0.0	0.0
6	0.0	0.5	0.0	0.0	0.0
7	0.0	1.275	0.0	0.0	0.0
8	0.0	1.525	0.0	0.0	0.0
9	0.0	1.8	0.0	0.0	0.0
10	0.0	2.15	0.0	0.0	0.0
11	0.0	2.475	0.0	0.0	0.0
12	0.0	3.0	0.0	0.0	0.0
13	0.0	3.4	0.0	0.0	0.0
14	0.0	3.8	0.0	0.0	0.0
15	0.0	4.5	0.0	0.0	0.0
16	0.0	4.95	0.0	0.0	0.0
17	0.0	5.7	0.0	0.0	0.0
18	0.0	6.8	0.0	0.0	0.0
19	0.0	8.2	0.0	0.0	0.0
20	0.0	9.125	0.0	0.0	0.0
21	0.0	10.75	0.0	0.0	0.0
22	0.0	12.1	0.0	0.0	0.0
23	0.0	13.775	0.0	0.0	0.0
24	0.0	14.925	0.0	0.0	0.0
25	0.0	17.5	0.0	0.0	0.0
26	0.0	19.875	0.0	0.0	0.0
27	0.0	27.5	0.0	0.0	0.0
28	0.0	36.45	0.0	0.0	0.0
29	0.0	42.375	0.0	0.0	0.0
30	0.0	49.05	0.0	0.0	0.0
31	0.0	53.4	0.0	0.0	0.0
32	0.0	58.75	0.0	0.0	0.0
33	0.0	65.5	0.0	0.0	0.0
34	0.0	70.325	0.0	0.0	0.0
35	0.0	75.85	0.0	0.0	0.0
36	0.0	82.425	0.0	0.0	0.0
37	0.0	87.35	0.0	0.0	0.0
38	0.0	89.8	0.0	0.0	0.0
39	0.0	92.55	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAAAAC	25	0.0020355622	35.37	20
>>END_MODULE
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637596 READS because READLEN < 1
Read 1637596 spots for SRR9060564.sra
Written 1637596 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
Rejected 1637554 READS because READLEN < 1
Read 1637554 spots for SRR9060564.sra
Written 1637554 spots for SRR9060564.sra
SRR ids: ['SRR9060564.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_f67kohbv
SRR9060564.sra spots: 104803498
blocks: [[1, 1637554], [1637555, 3275108], [3275109, 4912662], [4912663, 6550216], [6550217, 8187770], [8187771, 9825324], [9825325, 11462878], [11462879, 13100432], [13100433, 14737986], [14737987, 16375540], [16375541, 18013094], [18013095, 19650648], [19650649, 21288202], [21288203, 22925756], [22925757, 24563310], [24563311, 26200864], [26200865, 27838418], [27838419, 29475972], [29475973, 31113526], [31113527, 32751080], [32751081, 34388634], [34388635, 36026188], [36026189, 37663742], [37663743, 39301296], [39301297, 40938850], [40938851, 42576404], [42576405, 44213958], [44213959, 45851512], [45851513, 47489066], [47489067, 49126620], [49126621, 50764174], [50764175, 52401728], [52401729, 54039282], [54039283, 55676836], [55676837, 57314390], [57314391, 58951944], [58951945, 60589498], [60589499, 62227052], [62227053, 63864606], [63864607, 65502160], [65502161, 67139714], [67139715, 68777268], [68777269, 70414822], [70414823, 72052376], [72052377, 73689930], [73689931, 75327484], [75327485, 76965038], [76965039, 78602592], [78602593, 80240146], [80240147, 81877700], [81877701, 83515254], [83515255, 85152808], [85152809, 86790362], [86790363, 88427916], [88427917, 90065470], [90065471, 91703024], [91703025, 93340578], [93340579, 94978132], [94978133, 96615686], [96615687, 98253240], [98253241, 99890794], [99890795, 101528348], [101528349, 103165902], [103165903, 104803498]]
SRR9060564 file size 14837744
SRR9060564 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 64 -o SRR9060564 SRR9060564_1.fastq
Input file:	SRR9060564_1.fastq
trimmed:	SRR9060564-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Thu Feb 13 06:41:10 2020 >> started

Thu Feb 13 06:43:03 2020 >> done (112.592s)
104803498 reads processed; of these:
        0 ( 0.00%) short reads filtered out after trimming by size control
        0 ( 0.00%) empty reads filtered out after trimming by size control
104803498 (100.00%) reads available; of these:
      848 ( 0.00%) trimmed reads available after processing
104802650 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 39	        1	  0.00%
 40	        3	  0.00%
 41	        8	  0.00%
 42	        0	  0.00%
 43	        0	  0.00%
 44	        9	  0.00%
 45	      930	  0.00%
 46	     1461	  0.00%
 47	     1540	  0.00%
 48	    34536	  0.03%
 49	  8669774	  8.27%
 50	 29964181	 28.59%
 51	 66131055	 63.10%
104803498 reads passed initial QC


criterion=sequence-density
sequence-density=93.30
sequence-density-rank=1
fanout-score=23.04
fanout-score-rank=1
prefix-density=95.81
prefix-fanout=22.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC


criterion=fanout-score
sequence-density=93.30
sequence-density-rank=1
fanout-score=23.04
fanout-score-rank=1
prefix-density=95.81
prefix-fanout=22.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 64 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC -o SRR9060564 -
Input file:	STDIN
trimmed:	SRR9060564-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	32
Thu Feb 13 06:49:04 2020 >> started

Thu Feb 13 06:52:16 2020 >> done (192.066s)
102573636 reads processed; of these:
  7622305 ( 7.43%) short reads filtered out after trimming by size control
   133706 ( 0.13%) empty reads filtered out after trimming by size control
 94817625 (92.44%) reads available; of these:
 93127370 (98.22%) trimmed reads available after processing
  1690255 ( 1.78%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 1641977	  1.73%
 19	 1006660	  1.06%
 20	 1554533	  1.64%
 21	 1710539	  1.80%
 22	 1690967	  1.78%
 23	 1383738	  1.46%
 24	 2900973	  3.06%
 25	 2658841	  2.80%
 26	 7492551	  7.90%
 27	 9233953	  9.74%
 28	 6558737	  6.92%
 29	 6709226	  7.08%
 30	 4341466	  4.58%
 31	 6007584	  6.34%
 32	 7101979	  7.49%
 33	 4512146	  4.76%
 34	 5761244	  6.08%
 35	 7775905	  8.20%
 36	 4867417	  5.13%
 37	 2114787	  2.23%
 38	 2847010	  3.00%
 39	 1137094	  1.20%
 40	  841917	  0.89%
 41	  539154	  0.57%
 42	   89683	  0.09%
 43	  455670	  0.48%
 44	   95109	  0.10%
 45	   24600	  0.03%
 46	   25658	  0.03%
 47	   22188	  0.02%
 48	   45926	  0.05%
 49	   83688	  0.09%
 50	  764582	  0.81%
 51	  820123	  0.86%


criterion=sequence-density
sequence-density=0.82
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=21
prefix-density=0.00
prefix-fanout=1.0
sequence=AGATGGTAGTTTATTGGACTACCATGGTTGTTACGGGTAACGGAGAATAAGGGTTCGACTCCGGAGAGGGAGCCTTAGAAACGGCTACCACGTCCAAGGAAGGCAGCAGGCGCGAAACTTATCCACTGTTGAGTATGAGATAGTGACTAAAAATATAAAGACTCATCCTT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=23
fanout-score=169.57
fanout-score-rank=1
prefix-density=3.44
prefix-fanout=1.0
sequence=CTAATTAAAACAGAGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGATTTCTGCCCAGTGCTCTGAATGTTAAATCGTAGTAATTCGAGTAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCC
                                 Started job on |	Feb 13 06:53:37
                             Started mapping on |	Feb 13 06:53:37
                                    Finished on |	Feb 13 06:56:49
       Mapping speed, Million of reads per hour |	1819.64

                          Number of input reads |	97047487
                      Average input read length |	30
                                    UNIQUE READS:
                   Uniquely mapped reads number |	55615820
                        Uniquely mapped reads % |	57.31%
                          Average mapped length |	29.62
                       Number of splices: Total |	500478
            Number of splices: Annotated (sjdb) |	413671
                       Number of splices: GT/AG |	438948
                       Number of splices: GC/AG |	2617
                       Number of splices: AT/AC |	568
               Number of splices: Non-canonical |	58345
                      Mismatch rate per base, % |	0.08%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.28
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.07
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	35665120
             % of reads mapped to multiple loci |	36.75%
        Number of reads mapped to too many loci |	2691034
             % of reads mapped to too many loci |	2.77%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.17%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	5766547	5766547	5766547
N_multimapping	35665120	35665120	35665120
N_noFeature	43353	53394	55544851
N_ambiguous	103589	51783	934
UnstrandedReadsAssigned:55468878 PositiveStrandReadsAssigned:55510643 NegativeStrandReadsAssigned:70035
Dataset is classified positive stranded
MeadianReadLen=30 20thPercentileLength=26 echo kmer=21
SRR9060564 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=21

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 21
[index] number of targets: 33,746
[index] number of k-mers: 28,900,170
[index] number of equivalence classes: 64,378
[quant] running in single-end mode
[quant] will process file 1: SRR9060564-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 97,047,487 reads, 9,952,490 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,284 rounds

  33747 SRR9060564.ke.tsv
  46739 SRR9060564.se.tsv
  80486 total
==> SRR9060564.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Y110A7A.10	1787	1688	115	5.67425
F27C8.1	1940	1841	26	1.17626
F07C3.7	1728	1629	22	1.12482
F52H2.2a	1739	1640	14	0.710996
F52H2.2b	1840	1741	0	0
T13A10.10a	1734	1635	21	1.06976
T13A10.10b	1598	1499	9.65392e-08	5.36396e-09
C55C2.5a	1840	1741	15.4012	0.736781
C55C2.5b	1606	1507	0	0

==> SRR9060564.se.tsv <==
WBGene00197333	0
WBGene00198386	0
WBGene00015153	6
WBGene00002061	2061
WBGene00255704	1
WBGene00235314	0
WBGene00001177	68
WBGene00169236	0
WBGene00219784	0
WBGene00015152	0
SRR9060564 completed mapping pipeline successfully
