Starting /dee2/code/volunteer_pipeline.sh SRR9060566
    current disk space = 823599652
    free memory = 121824728 
SRR9060566 SRAfilesize
a3a42803b8c70c8e798a429d027e0a86  SRR9060566.sra
SRR9060566.sra file validated
SRR9060566 is single end
SRR9060566 is conventional basespace
SRR9060566 read1 length is 46-51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR9060566_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	46-51
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1215	32.0	32.0	32.0	32.0	32.0
2	31.5205	32.0	32.0	32.0	32.0	32.0
3	31.67175	32.0	32.0	32.0	32.0	32.0
4	31.708	32.0	32.0	32.0	32.0	32.0
5	31.69725	32.0	32.0	32.0	32.0	32.0
6	34.582	36.0	36.0	36.0	36.0	36.0
7	35.30125	36.0	36.0	36.0	36.0	36.0
8	35.357	36.0	36.0	36.0	36.0	36.0
9	35.32925	36.0	36.0	36.0	36.0	36.0
10	35.38875	36.0	36.0	36.0	36.0	36.0
11	35.3945	36.0	36.0	36.0	36.0	36.0
12	35.406	36.0	36.0	36.0	36.0	36.0
13	35.4435	36.0	36.0	36.0	36.0	36.0
14	35.40875	36.0	36.0	36.0	36.0	36.0
15	35.413	36.0	36.0	36.0	36.0	36.0
16	35.47875	36.0	36.0	36.0	36.0	36.0
17	35.493	36.0	36.0	36.0	36.0	36.0
18	35.3765	36.0	36.0	36.0	36.0	36.0
19	35.35425	36.0	36.0	36.0	36.0	36.0
20	35.4225	36.0	36.0	36.0	36.0	36.0
21	35.39325	36.0	36.0	36.0	36.0	36.0
22	35.384	36.0	36.0	36.0	36.0	36.0
23	35.36825	36.0	36.0	36.0	36.0	36.0
24	35.31625	36.0	36.0	36.0	36.0	36.0
25	35.30325	36.0	36.0	36.0	36.0	36.0
26	35.40225	36.0	36.0	36.0	36.0	36.0
27	35.38375	36.0	36.0	36.0	36.0	36.0
28	35.518	36.0	36.0	36.0	36.0	36.0
29	35.411	36.0	36.0	36.0	36.0	36.0
30	35.2785	36.0	36.0	36.0	36.0	36.0
31	35.36825	36.0	36.0	36.0	36.0	36.0
32	35.3095	36.0	36.0	36.0	36.0	36.0
33	35.258	36.0	36.0	36.0	36.0	36.0
34	35.334	36.0	36.0	36.0	36.0	36.0
35	35.32525	36.0	36.0	36.0	36.0	36.0
36	35.28875	36.0	36.0	36.0	36.0	36.0
37	35.26825	36.0	36.0	36.0	36.0	36.0
38	35.292	36.0	36.0	36.0	36.0	36.0
39	35.0235	36.0	36.0	36.0	36.0	36.0
40	35.0785	36.0	36.0	36.0	36.0	36.0
41	35.1465	36.0	36.0	36.0	36.0	36.0
42	35.18725	36.0	36.0	36.0	36.0	36.0
43	35.1645	36.0	36.0	36.0	36.0	36.0
44	35.2475	36.0	36.0	36.0	36.0	36.0
45	35.11075	36.0	36.0	36.0	36.0	36.0
46	35.3155	36.0	36.0	36.0	36.0	36.0
47	35.13279879729391	36.0	36.0	36.0	36.0	36.0
48	35.02860727728984	36.0	36.0	36.0	36.0	36.0
49	34.98311917359536	36.0	36.0	36.0	36.0	36.0
50	35.064332247557005	36.0	36.0	36.0	36.0	36.0
51	34.94942903752039	36.0	36.0	36.0	36.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	2.0
24	3.0
25	5.0
26	11.0
27	24.0
28	31.0
29	41.0
30	57.0
31	72.0
32	103.0
33	167.0
34	424.0
35	3059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.575	39.975	15.049999999999999	28.4
2	13.725000000000001	31.674999999999997	37.375	17.224999999999998
3	23.9	17.525	38.925	19.650000000000002
4	34.65	18.125	25.474999999999998	21.75
5	47.85	19.950000000000003	16.5	15.7
6	23.816832949603477	19.979534407776924	40.95676643642875	15.246866206190843
7	29.5	19.3	19.5	31.7
8	25.85	30.349999999999998	18.175	25.624999999999996
9	23.7	34.025	27.825	14.45
10	35.875	18.575	27.55	18.0
11	45.35	18.224999999999998	20.05	16.375
12	18.325	17.875	42.0	21.8
13	35.425000000000004	19.875	26.625	18.075
14	19.15	19.6	44.9	16.35
15	29.825000000000003	14.774999999999999	38.65	16.75
16	40.425	18.625	19.5	21.45
17	29.349999999999998	18.35	34.525	17.775
18	29.975	31.624999999999996	21.45	16.950000000000003
19	22.75	28.249999999999996	33.650000000000006	15.35
20	24.625	18.45	32.625	24.3
21	30.349999999999998	18.8	20.325	30.525000000000002
22	21.275	33.875	21.625	23.225
23	35.099999999999994	18.975	28.525	17.4
24	20.724999999999998	16.6	42.25	20.424999999999997
25	20.625	34.425	26.25	18.7
26	34.55	20.150000000000002	19.3	26.0
27	24.55	31.15	29.325000000000003	14.975
28	46.125	20.625	18.65	14.6
29	33.15	19.75	16.35	30.75
30	21.0	35.525	27.474999999999998	16.0
31	38.925	22.15	23.674999999999997	15.25
32	22.825	17.575	46.075	13.525
33	24.75	16.75	30.4	28.1
34	29.549999999999997	21.475	22.05	26.924999999999997
35	32.550000000000004	18.925	35.625	12.9
36	24.325	25.55	38.425	11.700000000000001
37	32.375	27.1	30.375000000000004	10.15
38	35.949999999999996	19.55	32.800000000000004	11.700000000000001
39	25.724999999999998	26.775	33.175	14.325
40	18.075	30.85	28.1	22.975
41	19.475	18.95	41.6	19.975
42	20.349999999999998	10.75	39.675	29.225
43	30.45	7.5249999999999995	29.9	32.125
44	35.875	7.9	30.475	25.75
45	39.025	8.475000000000001	22.6	29.9
46	44.425	7.3999999999999995	22.575	25.6
47	55.44976196441994	9.87221247807567	14.106740165372086	20.571285392132296
48	46.72521957340025	15.407779171894607	11.141781681304893	26.72521957340025
49	34.29075333837239	18.56890904509952	19.148400100781053	27.99193751574704
50	37.269272529858846	20.874049945711185	15.363735070575462	26.492942453854507
51	34.09461663947798	0.0	16.68026101141925	49.225122349102776
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	1.5
22	2.0
23	1.5
24	1.0
25	1.5
26	3.0
27	3.5
28	2.5
29	2.0
30	1.5
31	3.0
32	6.0
33	5.5
34	4.5
35	5.0
36	8.0
37	11.5
38	23.5
39	36.5
40	52.0
41	76.0
42	94.0
43	109.5
44	220.5
45	341.5
46	427.0
47	507.0
48	525.0
49	549.0
50	491.0
51	406.5
52	443.5
53	633.5
54	773.5
55	649.0
56	445.5
57	325.5
58	249.0
59	188.0
60	144.5
61	102.5
62	53.0
63	21.0
64	17.0
65	11.5
66	6.0
67	3.5
68	3.0
69	2.5
70	1.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.275
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
46	9.0
47	6.0
48	16.0
49	285.0
50	1232.0
51	2452.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.92970337261276	57.175
2	3.04754164973588	3.75
3	1.5034538805363673	2.775
4	0.5282405526208858	1.3
5	0.3657049979683055	1.125
6	0.16253555465258024	0.6
7	0.16253555465258024	0.7000000000000001
8	0.12190166598943519	0.6
9	0.12190166598943519	0.675
>10	0.8126777732629012	11.325000000000001
>50	0.12190166598943519	4.725
>100	0.12190166598943519	15.25
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATTGTGCTTGCTTGCGGACGCTTTCTGGTGTTGGAATTCTCGGGTGCCA	219	5.475	No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTTGGAATTCTCGGGTG	197	4.925	No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTGC	194	4.8500000000000005	No Hit
ATACTGCGATCTGTTGAGACTATCCTTTGATTGAGTGGAATTCTCGGGTGC	68	1.7000000000000002	No Hit
AAGAGAAGGTGTCTTGCTGGCGGTAGTGGAATTCTCGGGTGCCAAGGAACT	67	1.675	RNA PCR Primer, Index 1 (100% over 25bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGCC	54	1.35	No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTTTGGAATTCTCGGGT	48	1.2	No Hit
TTGCGGACGCTTTCTGGTGTGTGCTTGGACCTTGGAATTCTCGGGTGCCA	42	1.05	No Hit
CCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCC	36	0.8999999999999999	No Hit
GATTGTGCTTGCTTGCGGACGCTTTCTGGTTGGAATTCTCGGGTGCCAAGG	29	0.7250000000000001	Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCA	28	0.7000000000000001	No Hit
CGGGGTGCAATTATTAGAACAGACCAAACTGGAATTCTCGGGTGCCAAGG	27	0.675	Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCA	24	0.6	No Hit
GTGCTTGGACCTCGGTTCTAGTATCCTGATCGCTCTGGAATTCTCGGGTGC	23	0.575	No Hit
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTTGGAATTCTCGGGTGCCA	23	0.575	No Hit
TTGCGGACGCTTTCTGGTGTGTGCTTGGACCTCTGGAATTCTCGGGTGCC	23	0.575	No Hit
GATTGTGCTTGCTTGCGGACGCTTTCTGGTGTGGAATTCTCGGGTGCCA	22	0.5499999999999999	No Hit
AGTGATTGTGCTTGCTTGCGGACGCTTTCTGGTGTTGGAATTCTCGGGTGC	21	0.525	No Hit
TTGTGATCGTTGCCGGGTGTCGTTTCCTTGGAATTCTCGGGTGCCAAGGA	19	0.475	RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTTTTGGAATTCTCGGG	16	0.4	No Hit
GGCCAGTTGGTTGATGCTTGTCCGGCGCAGTTCTGGAATTCTCGGGTGCC	14	0.35000000000000003	No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG	13	0.325	Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	12	0.3	RNA PCR Primer, Index 7 (100% over 51bp)
GGCCAGTTGGTTGATGCTTGTCCGGCGCAGTGGAATTCTCGGGTGCCAAGG	12	0.3	Illumina Small RNA Adapter 2 (100% over 21bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG	11	0.27499999999999997	No Hit
ACCGCCCGTCGCTATCCGGGACTGAACTGATTCTGGAATTCTCGGGTGCC	10	0.25	No Hit
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTCTGGAATTCTCGGGTGCCA	9	0.22499999999999998	No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTTGGAATTCTCGGGT	9	0.22499999999999998	No Hit
GTGCTTGGACCTCGGTTCTAGTATCCTGATCGCTTGGAATTCTCGGGTGCC	9	0.22499999999999998	No Hit
ATTGTGCTTGCTTGCGGACGCTTTCTGGTGTTGGAATTCTCGGGTGCCA	8	0.2	No Hit
TGTGATCGTTGCCGGGTGTCGTTTCCTTGGAATTCTCGGGTGCCAAGGAAC	8	0.2	RNA PCR Primer, Index 1 (100% over 24bp)
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG	8	0.2	Illumina Small RNA Adapter 2 (100% over 21bp)
GGCCAGTTGGTTGATGCTTGTCCGGCGCAGTTCTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
GTGCTTGGACCTCGGTTCTAGTATCCTGATCGCTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
ACTTTCGGGTTGATGGCGGACTAGTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
GATTGNGCTTGCTTGCGGACGCTTTCTGGTGTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
CTTGGTCAAGGTGTTGTATTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC	6	0.15	No Hit
AAGCGGTGTTTAGCCGCACGAGATTGAGCGATAACTGGAATTCTCGGGTGC	6	0.15	No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTTTGTGGAATTCTCGGGTGCC	5	0.125	No Hit
AAGGCGGGGTGCAATTATTAGAACAGACCAAACTGGAATTCTCGGGTGCC	5	0.125	No Hit
ATTGTGCTTGCTTGCGGACGCTTTCTGGTGTGGAATTCTCGGGTGCCAAGG	5	0.125	Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGTGAAGAGTCATGAGAGGTGTAGCTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
TCTCAACCTGAACTCAGTCGTGATTACCCGCTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
CTTGGTCAAGGTGTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	5	0.125	RNA PCR Primer, Index 2 (100% over 34bp)
GCTTGCTTGCGGACGCTTTCTGGTGTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
ACCCATAAGGCCGGGGTTCGATTCCCCGCAACGGAACTGGAATTCTCGGGT	5	0.125	No Hit
GCTTGCGGACGCTTTCTGGTGTGTGCTTGGACCTCTGGAATTCTCGGGTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.375	0.0	0.0	0.0
2	0.0	0.375	0.0	0.0	0.0
3	0.0	0.375	0.0	0.0	0.0
4	0.0	0.4	0.0	0.0	0.0
5	0.0	0.45	0.0	0.0	0.0
6	0.0	0.725	0.0	0.0	0.0
7	0.0	1.325	0.0	0.0	0.0
8	0.0	2.1	0.0	0.0	0.0
9	0.0	2.925	0.0	0.0	0.0
10	0.0	3.225	0.0	0.0	0.0
11	0.0	3.3	0.0	0.0	0.0
12	0.0	3.55	0.0	0.0	0.0
13	0.0	3.75	0.0	0.0	0.0
14	0.0	3.9	0.0	0.0	0.0
15	0.0	4.25	0.0	0.0	0.0
16	0.0	4.525	0.0	0.0	0.0
17	0.0	4.775	0.0	0.0	0.0
18	0.0	5.05	0.0	0.0	0.0
19	0.0	5.3	0.0	0.0	0.0
20	0.0	5.525	0.0	0.0	0.0
21	0.0	5.675	0.0	0.0	0.0
22	0.0	6.075	0.0	0.0	0.0
23	0.0	6.4	0.0	0.0	0.0
24	0.0	6.625	0.0	0.0	0.0
25	0.0	7.125	0.0	0.0	0.0
26	0.0	8.1	0.0	0.0	0.0
27	0.0	11.4	0.0	0.0	0.0
28	0.0	13.475	0.0	0.0	0.0
29	0.0	15.7	0.0	0.0	0.0
30	0.0	19.6	0.0	0.0	0.0
31	0.0	24.85	0.0	0.0	0.0
32	0.0	31.225	0.0	0.0	0.0
33	0.0	45.2	0.0	0.0	0.0
34	0.0	54.175	0.0	0.0	0.0
35	0.0	62.05	0.0	0.0	0.0
36	0.0	77.75	0.0	0.0	0.0
37	0.0	88.875	0.0	0.0	0.0
38	0.0	93.975	0.0	0.0	0.0
39	0.0	96.425	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTGTG	60	0.0	44.911392	1
GTCCTTT	30	1.847844E-6	44.350002	31
ATTGTGC	65	0.0	41.456673	2
TTGTGCT	65	0.0	41.456673	3
GCTTGCT	65	0.0	40.93846	7
CTTGCTT	65	0.0	40.93846	8
TTGCTTG	65	0.0	40.93846	9
TGGTCAA	75	0.0	38.92321	3
TCAAGGT	75	0.0	38.92321	6
GTCAAGG	75	0.0	38.92321	5
GGTCAAG	75	0.0	38.92321	4
CTTGGTC	75	0.0	38.92321	1
TTGGTCA	75	0.0	38.92321	2
TGTTGTA	75	0.0	38.43667	12
GTAGAGC	75	0.0	38.43667	23
GTGTTGT	75	0.0	38.43667	11
TTGTATT	75	0.0	38.43667	14
AGTAGAG	75	0.0	38.43667	22
CAGTAGA	75	0.0	38.43667	21
GTATTCA	75	0.0	38.43667	16
>>END_MODULE
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464241 READS because READLEN < 1
Read 464241 spots for SRR9060566.sra
Written 464241 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
Rejected 464216 READS because READLEN < 1
Read 464216 spots for SRR9060566.sra
Written 464216 spots for SRR9060566.sra
SRR ids: ['SRR9060566.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_b0g_2usc
SRR9060566.sra spots: 29709849
blocks: [[1, 464216], [464217, 928432], [928433, 1392648], [1392649, 1856864], [1856865, 2321080], [2321081, 2785296], [2785297, 3249512], [3249513, 3713728], [3713729, 4177944], [4177945, 4642160], [4642161, 5106376], [5106377, 5570592], [5570593, 6034808], [6034809, 6499024], [6499025, 6963240], [6963241, 7427456], [7427457, 7891672], [7891673, 8355888], [8355889, 8820104], [8820105, 9284320], [9284321, 9748536], [9748537, 10212752], [10212753, 10676968], [10676969, 11141184], [11141185, 11605400], [11605401, 12069616], [12069617, 12533832], [12533833, 12998048], [12998049, 13462264], [13462265, 13926480], [13926481, 14390696], [14390697, 14854912], [14854913, 15319128], [15319129, 15783344], [15783345, 16247560], [16247561, 16711776], [16711777, 17175992], [17175993, 17640208], [17640209, 18104424], [18104425, 18568640], [18568641, 19032856], [19032857, 19497072], [19497073, 19961288], [19961289, 20425504], [20425505, 20889720], [20889721, 21353936], [21353937, 21818152], [21818153, 22282368], [22282369, 22746584], [22746585, 23210800], [23210801, 23675016], [23675017, 24139232], [24139233, 24603448], [24603449, 25067664], [25067665, 25531880], [25531881, 25996096], [25996097, 26460312], [26460313, 26924528], [26924529, 27388744], [27388745, 27852960], [27852961, 28317176], [28317177, 28781392], [28781393, 29245608], [29245609, 29709849]]
SRR9060566 file size 4186016
SRR9060566 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 64 -o SRR9060566 SRR9060566_1.fastq
Input file:	SRR9060566_1.fastq
trimmed:	SRR9060566-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Thu Feb 13 06:41:51 2020 >> started

Thu Feb 13 06:42:24 2020 >> done (32.567s)
29709849 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
29709849 (100.00%) reads available; of these:
     232 ( 0.00%) trimmed reads available after processing
29709617 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 40	       1	  0.00%
 41	       3	  0.00%
 42	       2	  0.00%
 43	       3	  0.00%
 44	       8	  0.00%
 45	     143	  0.00%
 46	   58666	  0.20%
 47	   54241	  0.18%
 48	   67046	  0.23%
 49	 2153858	  7.25%
 50	 9449423	 31.81%
 51	17926455	 60.34%
29709849 reads passed initial QC


criterion=sequence-density
sequence-density=96.57
sequence-density-rank=1
fanout-score=25.65
fanout-score-rank=1
prefix-density=97.90
prefix-fanout=25.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC


criterion=fanout-score
sequence-density=96.57
sequence-density-rank=1
fanout-score=25.65
fanout-score-rank=1
prefix-density=97.90
prefix-fanout=25.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 64 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC -o SRR9060566 -
Input file:	STDIN
trimmed:	SRR9060566-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	32
Thu Feb 13 06:43:44 2020 >> started

Thu Feb 13 06:44:33 2020 >> done (49.189s)
29097275 reads processed; of these:
 1385787 ( 4.76%) short reads filtered out after trimming by size control
   95933 ( 0.33%) empty reads filtered out after trimming by size control
27615555 (94.91%) reads available; of these:
27280948 (98.79%) trimmed reads available after processing
  334607 ( 1.21%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   73887	  0.27%
 19	   72238	  0.26%
 20	   70367	  0.25%
 21	  107261	  0.39%
 22	   77079	  0.28%
 23	   64400	  0.23%
 24	  165020	  0.60%
 25	  263871	  0.96%
 26	  858882	  3.11%
 27	  576801	  2.09%
 28	  705841	  2.56%
 29	 1110080	  4.02%
 30	 1416610	  5.13%
 31	 1940901	  7.03%
 32	 4109191	 14.88%
 33	 2930983	 10.61%
 34	 2458694	  8.90%
 35	 4328697	 15.67%
 36	 3221359	 11.67%
 37	 1569835	  5.68%
 38	  705102	  2.55%
 39	  229201	  0.83%
 40	   86614	  0.31%
 41	   65351	  0.24%
 42	   18486	  0.07%
 43	   22406	  0.08%
 44	   10892	  0.04%
 45	    6361	  0.02%
 46	    4651	  0.02%
 47	    5035	  0.02%
 48	    6994	  0.03%
 49	   23111	  0.08%
 50	  109824	  0.40%
 51	  199530	  0.72%


criterion=sequence-density
sequence-density=0.17
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=19
prefix-density=0.00
prefix-fanout=1.0
sequence=ACACAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATCACC


criterion=fanout-score
sequence-density=0.08
sequence-density-rank=10
fanout-score=25.44
fanout-score-rank=1
prefix-density=0.22
prefix-fanout=9.1
sequence=GGAATTCTCGGTGCCA
                                 Started job on |	Feb 13 06:45:28
                             Started mapping on |	Feb 13 06:45:28
                                    Finished on |	Feb 13 06:46:39
       Mapping speed, Million of reads per hour |	1431.29

                          Number of input reads |	28228129
                      Average input read length |	33
                                    UNIQUE READS:
                   Uniquely mapped reads number |	18650015
                        Uniquely mapped reads % |	66.07%
                          Average mapped length |	32.44
                       Number of splices: Total |	1183689
            Number of splices: Annotated (sjdb) |	1166585
                       Number of splices: GT/AG |	1171484
                       Number of splices: GC/AG |	5622
                       Number of splices: AT/AC |	135
               Number of splices: Non-canonical |	6448
                      Mismatch rate per base, % |	0.09%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.29
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.03
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	8406046
             % of reads mapped to multiple loci |	29.78%
        Number of reads mapped to too many loci |	665921
             % of reads mapped to too many loci |	2.36%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.79%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1172068	1172068	1172068
N_multimapping	8406046	8406046	8406046
N_noFeature	94124	100946	18542515
N_ambiguous	119916	19951	244
UnstrandedReadsAssigned:18435975 PositiveStrandReadsAssigned:18529118 NegativeStrandReadsAssigned:107256
Dataset is classified positive stranded
MeadianReadLen=33 20thPercentileLength=30 echo kmer=25
SRR9060566 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=25

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 25
[index] number of targets: 33,746
[index] number of k-mers: 28,932,940
[index] number of equivalence classes: 56,957
[quant] running in single-end mode
[quant] will process file 1: SRR9060566-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 28,228,129 reads, 12,475,529 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,340 rounds

  33747 SRR9060566.ke.tsv
  46739 SRR9060566.se.tsv
  80486 total
==> SRR9060566.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Y110A7A.10	1787	1688	461	19.4233
F27C8.1	1940	1841	55	2.12472
F07C3.7	1728	1629	31	1.35342
F52H2.2a	1739	1640	45.5248	1.97423
F52H2.2b	1840	1741	34.4752	1.40832
T13A10.10a	1734	1635	60.1103	2.61472
T13A10.10b	1598	1499	12.8897	0.611554
C55C2.5a	1840	1741	37.2009	1.51967
C55C2.5b	1606	1507	10.7988	0.509632

==> SRR9060566.se.tsv <==
WBGene00197333	0
WBGene00198386	0
WBGene00015153	7
WBGene00002061	6780
WBGene00255704	1
WBGene00235314	0
WBGene00001177	330
WBGene00169236	0
WBGene00219784	0
WBGene00015152	2
SRR9060566 completed mapping pipeline successfully
