Starting /dee2/code/volunteer_pipeline.sh SRR13919965
    current disk space = 685274760
    free memory = 28183108 
SRR13919965 SRAfilesize
7d1fff0d0bda08ee771b8f66c292841a  SRR13919965.sra
SRR13919965.sra file validated
SRR13919965 is paired end
SRR13919965 is conventional basespace
SRR13919965 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13919965_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33625	32.0	32.0	32.0	32.0	32.0
2	31.5675	32.0	32.0	32.0	32.0	32.0
3	35.34625	37.0	37.0	37.0	32.0	37.0
4	36.02375	37.0	37.0	37.0	32.0	37.0
5	36.35875	37.0	37.0	37.0	37.0	37.0
6	39.956	41.0	41.0	41.0	37.0	41.0
7	40.10525	41.0	41.0	41.0	37.0	41.0
8	39.9475	41.0	41.0	41.0	37.0	41.0
9	40.0055	41.0	41.0	41.0	37.0	41.0
10-14	39.9936	41.0	41.0	41.0	37.0	41.0
15-19	40.07684999999999	41.0	41.0	41.0	37.0	41.0
20-24	39.926300000000005	41.0	41.0	41.0	37.0	41.0
25-29	39.92675	41.0	41.0	41.0	37.0	41.0
30-34	39.828199999999995	41.0	41.0	41.0	37.0	41.0
35-39	39.686699999999995	41.0	41.0	41.0	37.0	41.0
40-44	39.74765	41.0	41.0	41.0	37.0	41.0
45-49	39.694	41.0	41.0	41.0	37.0	41.0
50-54	39.59435	41.0	41.0	41.0	37.0	41.0
55-59	39.474650000000004	41.0	41.0	41.0	37.0	41.0
60-64	39.31235	41.0	41.0	41.0	37.0	41.0
65-69	39.3815	41.0	41.0	41.0	37.0	41.0
70-74	39.41435	41.0	41.0	41.0	37.0	41.0
75-79	39.09875	41.0	40.2	41.0	35.0	41.0
80-84	39.35725	41.0	41.0	41.0	37.0	41.0
85-89	39.4269	41.0	41.0	41.0	37.0	41.0
90-94	39.425200000000004	41.0	41.0	41.0	37.0	41.0
95-99	39.472699999999996	41.0	41.0	41.0	37.0	41.0
100-104	39.17115	41.0	41.0	41.0	36.0	41.0
105-109	39.1314	41.0	41.0	41.0	37.0	41.0
110-114	39.1077	41.0	41.0	41.0	36.0	41.0
115-119	38.86165	41.0	41.0	41.0	33.0	41.0
120-124	38.68035	41.0	41.0	41.0	32.0	41.0
125-129	38.470949999999995	41.0	38.6	41.0	32.0	41.0
130-134	38.4486	41.0	39.4	41.0	32.0	41.0
135-139	38.2222	41.0	37.8	41.0	32.0	41.0
140-144	38.040800000000004	41.0	37.8	41.0	32.0	41.0
145-149	37.76535	41.0	37.0	41.0	29.0	41.0
150	35.62375	41.0	37.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1201	1	0.0
1201	2	0.0
1201	3	0.0
1201	4	0.0
1201	5	0.0
1201	6	0.0
1201	7	0.0
1201	8	0.0
1201	9	0.0
1201	10-14	0.0
1201	15-19	0.0
1201	20-24	0.0
1201	25-29	0.0
1201	30-34	0.0
1201	35-39	0.0
1201	40-44	0.0
1201	45-49	0.0
1201	50-54	0.0
1201	55-59	0.0
1201	60-64	0.0
1201	65-69	0.0
1201	70-74	0.0
1201	75-79	0.0
1201	80-84	0.0
1201	85-89	0.0
1201	90-94	0.0
1201	95-99	0.0
1201	100-104	0.0
1201	105-109	0.0
1201	110-114	0.0
1201	115-119	0.0
1201	120-124	0.0
1201	125-129	0.0
1201	130-134	0.0
1201	135-139	0.0
1201	140-144	0.0
1201	145-149	0.0
1201	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	2.0
24	4.0
25	8.0
26	18.0
27	13.0
28	18.0
29	25.0
30	34.0
31	38.0
32	62.0
33	67.0
34	76.0
35	109.0
36	141.0
37	221.0
38	261.0
39	480.0
40	2422.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.49178255372946	20.40455120101138	12.187104930467763	25.9165613147914
2	14.774999999999999	20.025000000000002	38.65	26.55
3	17.25	24.349999999999998	30.325000000000003	28.075
4	13.25	16.1	39.574999999999996	31.075000000000003
5	13.8	37.4	33.300000000000004	15.5
6	31.0	37.875	17.599999999999998	13.525
7	28.549999999999997	33.2	20.775	17.474999999999998
8	25.6	33.15	22.125	19.125
9	28.199999999999996	13.8	20.849999999999998	37.15
10-14	25.435000000000002	23.165	27.474999999999998	23.925
15-19	23.87	27.74	24.735	23.655
20-24	24.795	26.045	25.205	23.955000000000002
25-29	24.23	27.1	24.85	23.82
30-34	24.67	25.064999999999998	26.119999999999997	24.145
35-39	25.47	26.56	23.95	24.02
40-44	23.799999999999997	25.415	25.435000000000002	25.35
45-49	24.21	25.66	25.990000000000002	24.14
50-54	24.727254529076166	25.953358022219998	25.01251126013412	24.306876188569714
55-59	23.794999999999998	26.700000000000003	25.580000000000002	23.925
60-64	24.59844883662747	25.809357017763325	26.039529647235426	23.55266449837378
65-69	24.056202810140505	26.00630031501575	25.336266813340668	24.601230061503074
70-74	24.5	24.415	27.185	23.9
75-79	23.605901475368842	25.501375343835956	26.756689172293076	24.136034008502126
80-84	25.48264479343803	25.897769330799242	23.857157147144143	24.762428728618584
85-89	23.927392739273927	25.95259525952595	25.57255725572557	24.547454745474546
90-94	23.865	25.53	25.53	25.074999999999996
95-99	25.04625231261563	24.751237561878096	25.02125106255313	25.18125906295315
100-104	24.07120356017801	24.8112405620281	25.20126006300315	25.91629581479074
105-109	23.70618530926546	25.386269313465675	26.021301065053255	24.88624431221561
110-114	23.9	26.045	24.975	25.080000000000002
115-119	23.28	26.169999999999998	25.615	24.935
120-124	24.17741774177418	26.74767476747675	24.547454745474546	24.527452745274527
125-129	22.994598919783957	26.54530906181236	25.875175035007004	24.584916983396678
130-134	23.848577286592988	25.70885632844927	25.20378056708506	25.23878581787268
135-139	23.789757951590317	26.455291058211643	25.12002400480096	24.63492698539708
140-144	23.409681936387276	25.89517903580716	25.985197039407883	24.70994198839768
145-149	23.565891472868216	26.866716679169794	25.046261565391347	24.521130282570645
150	23.380845211302827	25.6064016004001	23.1807951987997	27.831957989497376
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.5
11	1.0
12	0.5
13	0.0
14	0.5
15	0.5
16	0.0
17	0.0
18	0.5
19	0.5
20	0.5
21	0.5
22	0.5
23	1.5
24	1.0
25	1.0
26	1.0
27	2.0
28	5.5
29	8.0
30	7.5
31	9.0
32	12.0
33	15.5
34	24.0
35	24.5
36	22.0
37	27.0
38	44.0
39	46.5
40	52.0
41	80.5
42	88.5
43	98.5
44	113.0
45	155.5
46	220.0
47	262.5
48	309.0
49	311.0
50	313.5
51	322.5
52	289.5
53	259.5
54	211.0
55	156.5
56	135.5
57	108.5
58	89.5
59	81.5
60	48.0
61	22.5
62	8.5
63	2.5
64	1.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.125
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.09
55-59	0.0
60-64	0.075
65-69	0.005
70-74	0.0
75-79	0.025
80-84	0.03
85-89	0.01
90-94	0.0
95-99	0.005
100-104	0.005
105-109	0.005
110-114	0.0
115-119	0.0
120-124	0.01
125-129	0.02
130-134	0.015
135-139	0.02
140-144	0.02
145-149	0.025
150	0.025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.599999999999994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.23154362416108	43.05
2	13.926174496644295	16.6
3	5.704697986577181	10.2
4	2.6006711409395975	6.2
5	1.761744966442953	5.25
6	1.1325503355704698	4.05
7	0.6291946308724832	2.625
8	0.5453020134228188	2.6
9	0.587248322147651	3.15
>10	0.8808724832214765	6.275
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATAGGTCTCTCGCTTAAGCGCCATCCATTTTCAGGGCTAGTTGATTCG	19	0.475	No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTAATGCGGATATCGGTACGATTGA	15	0.375	No Hit
TCCAACTACTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAAGGATC	15	0.375	No Hit
CCCTAACTGAGATGCAAAGATTGTGTTCTTCGGAGCACAAGCGCGGTAAC	14	0.35000000000000003	No Hit
GTCTTTGAGCGACTAACCAAGTTCGAATGCGTTACCGCGCTTGTGCTCCG	13	0.325	No Hit
GTCTAGCGCCAGGTCGTAACGAGTAGGAAGGTCGTGGCGGTTGCGTTGAA	13	0.325	No Hit
TATTAACCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGCTTTTCG	13	0.325	No Hit
CCAGAAAGCGTCCGCAAGCAAGCACAATCACTAGTCCGCCATCAACCCGA	13	0.325	No Hit
GACCTATACTCCGCCGTTGCGACATGTGCGTTGTCTAGCGCCAGGTCGTA	12	0.3	No Hit
GTCTTTGGCTGCTTCGAAATGTGGCGTATAGGTGTAAGTTTCCAGCAGTG	11	0.27499999999999997	No Hit
GTGCAGAGGTTGAGCAGTTGGCAAACGACCCGAAAGATGGTGAACTATGC	11	0.27499999999999997	No Hit
ATCCTAAGGTACTGGCGAACGCCTTGTATCATCGGTGGCGAAAAGCTTGC	11	0.27499999999999997	No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCA	11	0.27499999999999997	No Hit
GCGCTAGACAACGCACATGTCGCAACGGCGGAGTATAGGTCTCTCGCTTA	10	0.25	No Hit
CCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACC	10	0.25	No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCTATCGAT	10	0.25	No Hit
GTAGTTGGATGTGGGTCAGTCGATCCTAAGGTACTGGCGAACGCCTTGTA	10	0.25	No Hit
GTCACAAGTGACACGCACAGGGCCGTGGTGCGCTGCACTCCGAAGAACAC	10	0.25	No Hit
ACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTAATGCGGAT	10	0.25	No Hit
GGGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGGAGCACA	10	0.25	No Hit
CATAAAAGGTGTTGGTTGATATTGACAGCAGGACGGTGGCCATGGAAGTC	10	0.25	No Hit
ATCTAGTAGCTGGTTCCTTCCGAAGTTTCCCTCAGGATAGCTGGATCTCA	9	0.22499999999999998	No Hit
TTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGAGACAC	9	0.22499999999999998	No Hit
TTCCTATGCTACGCCGACGGCGTTGGCTGCTCGTTCTAGCCCGACAGTGT	9	0.22499999999999998	No Hit
CAGTAGTTGGATGTGGGTCAGTCGATCCTAAGGTACTGGCGAACGCCTTG	9	0.22499999999999998	No Hit
TTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACCGCGGTGCTTT	9	0.22499999999999998	No Hit
GTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGG	9	0.22499999999999998	No Hit
CCCTTAGTAACGGCGAGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCA	9	0.22499999999999998	No Hit
CAATCGTACCGATATCCGCATTAGGTCTCCAAGGTGAACAGCCTCTAGTC	9	0.22499999999999998	No Hit
GTTTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACCGCGGTGCT	9	0.22499999999999998	No Hit
CCTTAGGATCGACTGACCCACATCCAACTACTGTTCACGTGGAACCCTTC	9	0.22499999999999998	No Hit
GCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAACTAGATC	9	0.22499999999999998	No Hit
CCTTTTATGGGGTCTCATGAGCGAGAAGTTAGGCACCTTAACTCCACGTT	9	0.22499999999999998	No Hit
CACTAGCGTAACTAAACGCTAGCCGCCACGAACCCACTACCGCCAGCAAG	9	0.22499999999999998	No Hit
CTCCTTAGCGGATACCGACTTCCATGGCCACCGTCCTGCTGTCAATATCA	9	0.22499999999999998	No Hit
CACGTTGACAGTTCACTAAAGAAACTGCAACTTCATATCCATTGAAAGTT	8	0.2	No Hit
ACTTGTGACTGTGCAGAGGTTGAGCAGTTGGCAAACGACCCGAAAGATGG	8	0.2	No Hit
CCACGGCCCTGTGCGTGTCACTTGTGACTGTGCAGAGGTTGAGCAGTTGG	8	0.2	No Hit
CTCTTGAACTCTCTCTTCAAAGTCCTTTGCAACTTTCCCTCACGGTACTT	8	0.2	No Hit
ATATTAACCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGCTTTTC	8	0.2	No Hit
CCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGCTTTTCGCCACCG	8	0.2	No Hit
CAGTTGATGTTGCTTGGAGACAAGCTTGGTGACTGGTCGCTTAGTTGTGA	8	0.2	No Hit
GAATATAACTGCCTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAA	8	0.2	No Hit
GTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCAAGGACCATCTGTC	8	0.2	No Hit
AATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGAGAC	8	0.2	No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAAT	8	0.2	No Hit
GTTCAAAGAGCACGAGACACCACAGAAACCGCGGTGCTTTTCGGAAACAA	8	0.2	No Hit
GTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACC	8	0.2	No Hit
CATGAGACCCCATAAAAGGTGTTGGTTGATATTGACAGCAGGACGGTGGC	7	0.17500000000000002	No Hit
GAAGTGGAGAAGGGTTCCACGTGAACAGTAGTTGGATGTGGGTCAGTCGA	7	0.17500000000000002	No Hit
ATCCAGAACGGGGCTCGCACCCTCTCTGGTTTATGGCCTCAATCGTAAGG	7	0.17500000000000002	No Hit
CCTATACCCAAGTCTATCGATCGATTTGCACGTCAGAACCGATACGGACT	7	0.17500000000000002	No Hit
CGATGATACAAGGCGTTCGCCAGTACCTTAGGATCGACTGACCCACATCC	7	0.17500000000000002	No Hit
TTGTTACACACTCCTTAGCGGATACCGACTTCCATGGCCACCGTCCTGCT	7	0.17500000000000002	No Hit
TCTCTGGCTGAACCAATTCCAGGGAGTCCGTTCCTTAACTAGAGAAGAAA	7	0.17500000000000002	No Hit
GGCTATGAGCGTAGGCTCGGCTGGAGCTTCCGTCAGTGCAGATCGTAATG	7	0.17500000000000002	No Hit
GTAATGTCCTCAACCTATTCTCAAACTTTCAATGGATATGAAGTTGCAGT	7	0.17500000000000002	No Hit
GTCCTCAACCTATTCTCAAACTTTCAATGGATATGAAGTTGCAGTTTCTT	7	0.17500000000000002	No Hit
ATTCCAGGGAGTCCGTTCCTTAACTAGAGAAGAAAACTCTAGCTCGGTCT	7	0.17500000000000002	No Hit
CAGCAGGACGGTGGCCATGGAAGTCGGTATCCGCTAAGGAGTGTGTAACA	7	0.17500000000000002	No Hit
GTGTTCTTCGGAGTGCAGCGCACCACGGCCCTGTGCGTGTCACTTGTGAC	7	0.17500000000000002	No Hit
CCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGCGATCAGGA	7	0.17500000000000002	No Hit
ACGCTAGTGTGTGTGACGTCGGTGTGAAAGTCGACGACGTTTCCGACCCG	7	0.17500000000000002	No Hit
CTCTAGCTCGGTCTTTGAGCGACTAACCAAGTTCGAATGCGTTACCGCGC	6	0.15	No Hit
GTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGCGATCAGGATACTAG	6	0.15	No Hit
GAGTATAGGTCTCTCGCTTAAGCGCCATCCATTTTCAGGGCTAGTTGATT	6	0.15	No Hit
GTATCATCGGTGGCGAAAAGCTTGCTTTTAGTCCCCGCTTGTCGAAAGGG	6	0.15	No Hit
GTTACACACTCCTTAGCGGATACCGACTTCCATGGCCACCGTCCTGCTGT	6	0.15	No Hit
GTTATATTCGGTAAAGCTAATGATTAGAGGCCTTGGGGACGTAATGTCCT	6	0.15	No Hit
CCTTACGATTGAGGCCATAAACCAGAGAGGGTGCGAGCCCCGTTCTGGAT	6	0.15	No Hit
GCCTAAAGTGGGTGATAAACTTCATCTAAGGCTAAATATCGACTCGATTG	6	0.15	No Hit
TCCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACC	6	0.15	No Hit
GTCCTGCTGTCAATATCAACCAACACCTTTTATGGGGTCTCATGAGCGAG	6	0.15	No Hit
ACTAAACGCTAGCCGCCACGAACCCACTACCGCCAGCAAGACACCTTCTC	6	0.15	No Hit
GAATAATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGGGAA	6	0.15	No Hit
AACAAGTACCGTGAGGGAAAGTTGCAAAGGACTTTGAAGAGAGAGTTCAA	6	0.15	No Hit
ATCTCTGGCTGAACCAATTCCAGGGAGTCCGTTCCTTAACTAGAGAAGAA	6	0.15	No Hit
AAACCAGAGAGGGTGCGAGCCCCGTTCTGGATAGCGGCACTGTTGGTTCG	6	0.15	No Hit
GTCAACGTGAATGCGAGGTCCAAGTGGGCCATTTTTGGTAAGCAGAACTG	6	0.15	No Hit
GGATACTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCAA	6	0.15	No Hit
CCTTAGCGGATACCGACTTCCATGGCCACCGTCCTGCTGTCAATATCAAC	6	0.15	No Hit
GGTTGAGCAGTTGGCAAACGACCCGAAAGATGGTGAACTATGCCTGAGCA	6	0.15	No Hit
ATACAAGGCGTTCGCCAGTACCTTAGGATCGACTGACCCACATCCAACTA	6	0.15	No Hit
GGACATTACGTCCCCAAGGCCTCTAATCATTAGCTTTACCGAATATAACT	6	0.15	No Hit
CCTATACTCCGCCGTTGCGACATGTGCGTTGTCTAGCGCCAGGTCGTAAC	6	0.15	No Hit
CCTCTGCACAGTCACAAGTGACACGCACAGGGCCGTGGTGCGCTGCACTC	6	0.15	No Hit
CTATTAATTTGCACTCAATCGTACCGATATCCGCATTAGGTCTCCAAGGT	6	0.15	No Hit
GTTTCTGTGGTGTCTCGTGCTCTTTGAACGGCCCTTAAAACACCAAGGGA	6	0.15	No Hit
TACTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCAAGCA	6	0.15	No Hit
CCATTACGATCTGCACTGACGGAAGCTCCAGCCGAGCCTACGCTCATAGC	6	0.15	No Hit
GGCTATTAATTTGCACTCAATCGTACCGATATCCGCATTAGGTCTCCAAG	5	0.125	No Hit
GTTCTGACGTGCAAATCGATCGATAGACTTGGGTATAGGGGCGAAAGACT	5	0.125	No Hit
GACGTAATGTCCTCAACCTATTCTCAAACTTTCAATGGATATGAAGTTGC	5	0.125	No Hit
ACTTTAGGCTGCACTTTCAAGCAACCCGACTCCAAGGAGCAAGCGAACCA	5	0.125	No Hit
GTCACCAAGCTTGTCTCCAAGCAACATCAACTGTCTCCGGTTCCACTCCA	5	0.125	No Hit
CACCAGAAAGCGTCCGCAAGCAAGCACAATCACTAGTCCGCCATCAACCC	5	0.125	No Hit
GAACTGGCGCTGTGGGATGAACCAAACGTGGAGTTAAGGTGCCTAACTTC	5	0.125	No Hit
CACCAAGGGAGGCTATTAATTTGCACTCAATCGTACCGATATCCGCATTA	5	0.125	No Hit
ATATAACTGCCTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACC	5	0.125	No Hit
GTCGTATGTCCGAAGTCCTTACGATTGAGGCCATAAACCAGAGAGGGTGC	5	0.125	No Hit
GTTCACCTTGGAGACCTAATGCGGATATCGGTACGATTGAGTGCAAATTA	5	0.125	No Hit
AGTTGGATGTGGGTCAGTCGATCCTAAGGTACTGGCGAACGCCTTGTATC	5	0.125	No Hit
GCCCTGTGCGTGTCACTTGTGACTGTGCAGAGGTTGAGCAGTTGGCAAAC	5	0.125	No Hit
GGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGGAGCACAA	5	0.125	No Hit
CCTAATGCGGATATCGGTACGATTGAGTGCAAATTAATAGCCTCCCTTGG	5	0.125	No Hit
CACCAAGCTTGTCTCCAAGCAACATCAACTGTCTCCGGTTCCACTCCAGC	5	0.125	No Hit
CTTAAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTT	5	0.125	No Hit
CACTTGGACCTCGCATTCACGTTGACAGTTCACTAAAGAAACTGCAACTT	5	0.125	No Hit
AGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGGGAAAAGGATTGG	5	0.125	No Hit
TATCAACGCAGAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCAA	5	0.125	No Hit
GAACACAATCTTTGCATCTCAGTTAGGGAATATTAACCCTATTCCCTTTC	5	0.125	No Hit
GTTCAAGAGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGTTGATGTT	5	0.125	No Hit
TCTGTCACCTCTGTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCG	5	0.125	No Hit
CTCATAGCCTTCAACGCAACCGCCACGACCTTCCTACTCGTTACGACCTG	5	0.125	No Hit
GTGTTGCCCATCTCGCAAGAGAAGGTGTCTTGCTGGCGGTAGTGGGTTCG	5	0.125	No Hit
CCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAA	5	0.125	No Hit
GACTAAAAGCAAGCTTTTCGCCACCGATGATACAAGGCGTTCGCCAGTAC	5	0.125	No Hit
ACATGTGCGTTGTCTAGCGCCAGGTCGTAACGAGTAGGAAGGTCGTGGCG	5	0.125	No Hit
AGTGTGTGTGACGTCGGTGTGAAAGTCGACGACGTTTCCGACCCGTCTTG	5	0.125	No Hit
CAGTTATATTCGGTAAAGCTAATGATTAGAGGCCTTGGGGACGTAATGTC	5	0.125	No Hit
GTCGTAACGAGTAGGAAGGTCGTGGCGGTTGCGTTGAAGGCTATGAGCGT	5	0.125	No Hit
CACAATCACTAGTCCGCCATCAACCCGAAAGTATCAAGCAGACAGAACTG	5	0.125	No Hit
CCTCTGTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAG	5	0.125	No Hit
CCTTAACTAGAGAAGAAAACTCTAGCTCGGTCTTTGAGCGACTAACCAAG	5	0.125	No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCG	5	0.125	No Hit
AGTTACGCTAGTGTGTGTGACGTCGGTGTGAAAGTCGACGACGTTTCCGA	5	0.125	No Hit
GCATAGGAAACGACACCCGGCAACGATCACAACTAAGCGACCAGTCACCA	5	0.125	No Hit
GTGCTTGTCTACTGCGAGTCAAAGGGTGTTAAAACCTTGCGGCGAAATGA	5	0.125	No Hit
ATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGGGAAAAGGA	5	0.125	No Hit
GCTCAGGCATAGTTCACCATCTTTCGGGTCGTTTGCCAACTGCTCAACCT	5	0.125	No Hit
GTCTTGTAACACGGATTGCGGAGTGCTTGTCTACTGCGAGTCAAAGGGTG	5	0.125	No Hit
GTTCTGGATAGCGGCACTGTTGGTTCGCTTGCTCCTTGGAGTCGGGTTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0125	0.0
32-33	0.0	0.0	0.0	0.05	0.0
34-35	0.0	0.0	0.0	0.075	0.0
36-37	0.0	0.0	0.0	0.1125	0.0
38-39	0.0	0.0	0.0	0.2875	0.0
40-41	0.0	0.0	0.0	0.35	0.0
42-43	0.0	0.0	0.0	0.4625	0.0
44-45	0.0	0.0	0.0	0.5375000000000001	0.0
46-47	0.0	0.0	0.0	0.6125	0.0
48-49	0.0	0.0	0.0	0.825	0.0
50-51	0.0	0.0	0.0	1.05	0.0
52-53	0.0	0.0	0.0	1.1875	0.0
54-55	0.0	0.0	0.0	1.225	0.0
56-57	0.0	0.0	0.0	1.3875000000000002	0.0
58-59	0.0	0.0	0.0	1.6	0.0
60-61	0.0	0.0	0.0	1.8	0.0
62-63	0.0	0.0	0.0	1.975	0.0
64-65	0.0	0.0	0.0	2.0875000000000004	0.0
66-67	0.0	0.0	0.0	2.2375	0.0
68-69	0.0	0.0	0.0	2.425	0.0
70-71	0.0	0.0	0.0	2.5875000000000004	0.0
72-73	0.0	0.0	0.0	2.7375	0.0
74-75	0.0	0.0	0.0	2.8875	0.0
76-77	0.0	0.0	0.0	3.1875	0.0
78-79	0.0	0.0	0.0	3.5	0.0
80-81	0.0	0.0	0.0	3.6875	0.0
82-83	0.0	0.0	0.0	3.9375	0.0
84-85	0.0	0.0	0.0	4.125	0.0
86-87	0.0	0.0	0.0	4.324999999999999	0.0
88-89	0.0	0.0	0.0	4.4125	0.0
90-91	0.0	0.0	0.0	4.5625	0.0
92-93	0.0	0.0	0.0	4.65	0.0
94-95	0.0	0.0	0.0	4.875	0.0
96-97	0.0	0.0	0.0	5.0	0.0
98-99	0.0	0.0	0.0	5.225	0.0
100-101	0.0	0.0	0.0	5.4875	0.0
102-103	0.0	0.0	0.0	5.65	0.0
104-105	0.0	0.0	0.0	5.7875	0.0
106-107	0.0	0.0	0.0	6.0875	0.0
108-109	0.0	0.0	0.0	6.3375	0.0
110-111	0.0	0.0	0.0	6.5875	0.0
112-113	0.0	0.0	0.0	6.987500000000001	0.0
114-115	0.0	0.0	0.0	7.35	0.0
116-117	0.0	0.0	0.0	7.6375	0.0
118-119	0.0	0.0	0.0	7.9875	0.0
120-121	0.0	0.0	0.0	8.274999999999999	0.0
122-123	0.0	0.0	0.0	8.4875	0.0
124-125	0.0	0.0	0.0	8.625	0.0
126-127	0.0	0.0	0.0	8.850000000000001	0.0
128-129	0.0	0.0	0.0	9.1375	0.0
130-131	0.0	0.0	0.0	9.45	0.0
132-133	0.0	0.0	0.0	9.8125	0.0
134-135	0.0	0.0	0.0	10.2375	0.0
136-137	0.0	0.0	0.0	10.8125	0.0
138	0.0	0.0	0.0	11.15	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACACG	25	8.956223E-4	86.399994	9
GTCACAA	30	0.0018473949	72.0	1
AGTGACA	30	0.0018473949	72.0	7
GTGACAC	30	0.0018473949	72.0	8
ACAAGTG	30	0.0018473949	72.0	4
AAGTGAC	30	0.0018473949	72.0	6
CAAGTGA	30	0.0018473949	72.0	5
CACAAGT	35	0.0034045284	61.714283	3
TCACAAG	35	0.0034045284	61.714283	2
AAAAAAA	30	0.0015031899	23.999998	110-114
>>END_MODULE
SRR13919965 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13919965_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	2.0	2.0	2.0	2.0	2.0	2.0
2	31.26625	32.0	32.0	32.0	32.0	32.0
3	31.3675	32.0	32.0	32.0	32.0	32.0
4	35.26375	37.0	37.0	37.0	32.0	37.0
5	35.9025	37.0	37.0	37.0	32.0	37.0
6	39.1955	41.0	41.0	41.0	37.0	41.0
7	39.453	41.0	41.0	41.0	37.0	41.0
8	39.7165	41.0	41.0	41.0	37.0	41.0
9	39.6765	41.0	41.0	41.0	37.0	41.0
10-14	39.542100000000005	41.0	41.0	41.0	37.0	41.0
15-19	39.6582	41.0	41.0	41.0	37.0	41.0
20-24	39.6182	41.0	41.0	41.0	37.0	41.0
25-29	39.595800000000004	41.0	41.0	41.0	37.0	41.0
30-34	39.4925	41.0	41.0	41.0	37.0	41.0
35-39	39.506350000000005	41.0	41.0	41.0	37.0	41.0
40-44	39.5053	41.0	41.0	41.0	37.0	41.0
45-49	39.38315	41.0	41.0	41.0	37.0	41.0
50-54	39.3772	41.0	41.0	41.0	37.0	41.0
55-59	39.322950000000006	41.0	41.0	41.0	37.0	41.0
60-64	39.299899999999994	41.0	41.0	41.0	37.0	41.0
65-69	39.22285000000001	41.0	41.0	41.0	37.0	41.0
70-74	39.049499999999995	41.0	41.0	41.0	36.0	41.0
75-79	38.675599999999996	41.0	40.2	41.0	33.0	41.0
80-84	39.29045000000001	41.0	41.0	41.0	37.0	41.0
85-89	39.2668	41.0	41.0	41.0	37.0	41.0
90-94	39.245549999999994	41.0	41.0	41.0	37.0	41.0
95-99	39.0928	41.0	41.0	41.0	37.0	41.0
100-104	39.052499999999995	41.0	41.0	41.0	37.0	41.0
105-109	38.9614	41.0	41.0	41.0	37.0	41.0
110-114	38.833600000000004	41.0	41.0	41.0	36.0	41.0
115-119	38.685750000000006	41.0	41.0	41.0	35.0	41.0
120-124	38.376599999999996	41.0	41.0	41.0	32.0	41.0
125-129	38.00615	41.0	40.2	41.0	31.0	41.0
130-134	37.64445	41.0	37.8	41.0	30.0	41.0
135-139	37.770849999999996	41.0	40.2	41.0	28.0	41.0
140-144	37.400999999999996	41.0	37.8	41.0	27.0	41.0
145-149	36.991	41.0	37.0	41.0	27.0	41.0
150	31.28575	37.0	27.0	41.0	12.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1201	1	0.0
1201	2	0.0
1201	3	0.0
1201	4	0.0
1201	5	0.0
1201	6	0.0
1201	7	0.0
1201	8	0.0
1201	9	0.0
1201	10-14	0.0
1201	15-19	0.0
1201	20-24	0.0
1201	25-29	0.0
1201	30-34	0.0
1201	35-39	0.0
1201	40-44	0.0
1201	45-49	0.0
1201	50-54	0.0
1201	55-59	0.0
1201	60-64	0.0
1201	65-69	0.0
1201	70-74	0.0
1201	75-79	0.0
1201	80-84	0.0
1201	85-89	0.0
1201	90-94	0.0
1201	95-99	0.0
1201	100-104	0.0
1201	105-109	0.0
1201	110-114	0.0
1201	115-119	0.0
1201	120-124	0.0
1201	125-129	0.0
1201	130-134	0.0
1201	135-139	0.0
1201	140-144	0.0
1201	145-149	0.0
1201	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	4.0
16	7.0
17	10.0
18	8.0
19	10.0
20	10.0
21	15.0
22	11.0
23	17.0
24	13.0
25	21.0
26	24.0
27	20.0
28	22.0
29	30.0
30	33.0
31	18.0
32	32.0
33	65.0
34	69.0
35	102.0
36	136.0
37	141.0
38	268.0
39	553.0
40	2358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	NaN	NaN	NaN	NaN
2	15.375	22.225	38.3	24.099999999999998
3	16.625	25.124999999999996	30.775000000000002	27.474999999999998
4	14.099999999999998	15.125	38.15	32.625
5	13.900000000000002	36.65	33.75	15.7
6	29.75	37.4	18.175	14.674999999999999
7	28.714357178589296	32.266133066533264	21.635817908954476	17.38369184592296
8	26.400000000000002	33.050000000000004	20.849999999999998	19.7
9	28.499999999999996	14.774999999999999	20.65	36.075
10-14	24.977497749774976	23.632363236323634	27.032703270327037	24.357435743574356
15-19	24.159831966393277	27.145429085817163	24.71994398879776	23.9747949589918
20-24	25.112511251125113	26.307630763076308	24.372437243724374	24.207420742074206
25-29	23.819763952790556	26.585317063412685	25.090018003600722	24.50490098019604
30-34	24.35487097419484	25.340068013602718	25.79015803160632	24.51490298059612
35-39	24.88497699539908	26.295259051810362	24.749949989998	24.06981396279256
40-44	24.379875975195038	25.465093018603717	25.070014002800562	25.08501700340068
45-49	24.184836967393476	25.68513702740548	25.670134026805364	24.459891978395678
50-54	24.932479743923178	26.96809042712814	24.872461738521558	23.22696809042713
55-59	24.202260678203462	26.232869860958285	25.437631289386815	24.127238171451435
60-64	23.937181154346305	26.05781734520356	26.072821846553968	23.932179653896167
65-69	24.197259177753324	25.84775432629789	25.157547264179254	24.79743923176953
70-74	24.034613845538217	25.560224089635852	26.57062825130052	23.83453381352541
75-79	23.754501800720288	25.740296118447382	26.405562224889955	24.099639855942375
80-84	25.345138055222087	26.08543417366947	24.284713885554222	24.284713885554222
85-89	24.55613903475869	25.68642160540135	26.196549137284318	23.56089022255564
90-94	23.496748374187092	25.907953976988495	25.237618809404704	25.357678839419712
95-99	25.834041914670138	25.29885459910969	24.70864802680938	24.158455459410792
100-104	24.094637855142057	24.724889955982395	25.310124049619848	25.8703481392557
105-109	24.224689875950382	24.829931972789115	25.685274109643856	25.26010404161665
110-114	24.221055263815956	25.98149537384346	24.926231557889473	24.871217804451113
115-119	23.407022106631988	26.247874362308693	25.75772731819546	24.58737621286386
120-124	24.44477791116447	26.295518207282914	24.384753901560625	24.874949979991996
125-129	24.559823929571827	26.460584233693478	24.789915966386555	24.18967587034814
130-134	24.804921968787514	25.605242096838737	24.88995598239296	24.69987995198079
135-139	23.8371511453436	26.22286686005802	25.0925277583275	24.84745423627088
140-144	23.58707612283685	26.84305291587476	24.9074722416725	24.662398719615886
145-149	23.584716943388678	27.725545109021805	24.75495099019804	23.93478695739148
150	23.336668334167083	27.263631815907953	23.111555777888945	26.28814407203602
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	0.5
21	1.0
22	1.0
23	0.5
24	1.0
25	1.0
26	4.0
27	6.0
28	5.5
29	6.5
30	8.0
31	12.5
32	17.0
33	18.0
34	21.0
35	24.0
36	24.5
37	42.0
38	48.0
39	42.0
40	57.0
41	80.0
42	104.5
43	121.5
44	157.5
45	199.0
46	247.0
47	324.5
48	340.5
49	333.5
50	323.0
51	277.0
52	249.5
53	209.0
54	143.0
55	107.5
56	110.5
57	118.0
58	94.5
59	49.5
60	32.5
61	20.5
62	6.5
63	3.5
64	2.0
65	1.0
66	0.0
67	0.5
68	0.5
69	0.5
70	0.5
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	100.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.05
8	0.0
9	0.0
10-14	0.01
15-19	0.02
20-24	0.01
25-29	0.02
30-34	0.02
35-39	0.02
40-44	0.02
45-49	0.02
50-54	0.03
55-59	0.03
60-64	0.03
65-69	0.03
70-74	0.04
75-79	0.04
80-84	0.04
85-89	0.025
90-94	0.05
95-99	0.034999999999999996
100-104	0.04
105-109	0.04
110-114	0.025
115-119	0.03
120-124	0.04
125-129	0.04
130-134	0.04
135-139	0.03
140-144	0.03
145-149	0.02
150	0.05
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.84787018255578	46.125
2	11.561866125760648	14.249999999999998
3	6.247464503042596	11.55
4	2.4746450304259633	6.1
5	1.5821501014198782	4.875
6	1.0953346855983772	4.05
7	0.6896551724137931	2.9749999999999996
8	0.486815415821501	2.4
9	0.2028397565922921	1.125
>10	0.8113590263691683	6.550000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NTATCAACGCAGAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCA	21	0.525	No Hit
NTAGTTGGATGTGGGTCAGTCGATCCTAAGGTACTGGCGAACGCCTTGTA	18	0.44999999999999996	No Hit
NACCTATACTCCGCCGTTGCGACATGTGCGTTGTCTAGCGCCAGGTCGTA	17	0.42500000000000004	No Hit
NTCCTAAGGTACTGGCGAACGCCTTGTATCATCGGTGGCGAAAAGCTTGC	15	0.375	No Hit
NCCAACTACTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAAGGATC	15	0.375	No Hit
NCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAAT	15	0.375	No Hit
NCTTTTATGGGGTCTCATGAGCGAGAAGTTAGGCACCTTAACTCCACGTT	15	0.375	No Hit
NCTTAGGATCGACTGACCCACATCCAACTACTGTTCACGTGGAACCCTTC	14	0.35000000000000003	No Hit
NTATAGGTCTCTCGCTTAAGCGCCATCCATTTTCAGGGCTAGTTGATTCG	14	0.35000000000000003	No Hit
NCTGTCACCTCTGTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCG	12	0.3	No Hit
NTCACAAGTGACACGCACAGGGCCGTGGTGCGCTGCACTCCGAAGAACAC	12	0.3	No Hit
NTCTAGCGCCAGGTCGTAACGAGTAGGAAGGTCGTGGCGGTTGCGTTGAA	11	0.27499999999999997	No Hit
NCCTAAAGTGGGTGATAAACTTCATCTAAGGCTAAATATCGACTCGATTG	11	0.27499999999999997	No Hit
NTGCAGAGGTTGAGCAGTTGGCAAACGACCCGAAAGATGGTGAACTATGC	11	0.27499999999999997	No Hit
NTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCTATCGAT	11	0.27499999999999997	No Hit
NTTTTAACACCCTTTGACTCGCAGTAGACAAGCACTCCGCAATCCGTGTT	10	0.25	No Hit
NACTAGCGTAACTAAACGCTAGCCGCCACGAACCCACTACCGCCAGCAAG	10	0.25	No Hit
NTCTTTGAGCGACTAACCAAGTTCGAATGCGTTACCGCGCTTGTGCTCCG	10	0.25	No Hit
NGATACTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCAA	10	0.25	No Hit
NAATCGTACCGATATCCGCATTAGGTCTCCAAGGTGAACAGCCTCTAGTC	10	0.25	No Hit
NTATCATCGGTGGCGAAAAGCTTGCTTTTAGTCCCCGCTTGTCGAAAGGG	9	0.22499999999999998	No Hit
NCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAA	9	0.22499999999999998	No Hit
NTCCTTAGCGGATACCGACTTCCATGGCCACCGTCCTGCTGTCAATATCA	9	0.22499999999999998	No Hit
NTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGAACAGTAGTTGGATGTG	9	0.22499999999999998	No Hit
NTATTAACCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGCTTTTC	9	0.22499999999999998	No Hit
NCTTAACTCCACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAA	8	0.2	No Hit
NTTATATTCGGTAAAGCTAATGATTAGAGGCCTTGGGGACGTAATGTCCT	8	0.2	No Hit
NCACAGAAACCGCGGTGCTTTTCGGAAACAACGTCCCCATCTCTGGCTGA	8	0.2	No Hit
NATAAAAGGTGTTGGTTGATATTGACAGCAGGACGGTGGCCATGGAAGTC	8	0.2	No Hit
NATCAACGCAGAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCAA	8	0.2	No Hit
NTACATGGGTCTCAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAG	8	0.2	No Hit
NACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTAATG	8	0.2	No Hit
NCGCAGAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCAAGGACC	8	0.2	No Hit
NCTAGAGGCTGTTCACCTTGGAGACCTAATGCGGATATCGGTACGATTGA	8	0.2	No Hit
NCCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACC	8	0.2	No Hit
NTTCTCCACTTCAGTCTTCAAGGATCGAACTTGAATATTTGCTACTACCA	8	0.2	No Hit
NAATAATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGGGAA	8	0.2	No Hit
NACCAGAAAGCGTCCGCAAGCAAGCACAATCACTAGTCCGCCATCAACCC	7	0.17500000000000002	No Hit
NAGTATAGGTCTCTCGCTTAAGCGCCATCCATTTTCAGGGCTAGTTGATT	7	0.17500000000000002	No Hit
NCATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTAATGCGGAT	7	0.17500000000000002	No Hit
NTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACCGCGGTGCTTT	7	0.17500000000000002	No Hit
NTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACC	7	0.17500000000000002	No Hit
NAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGT	7	0.17500000000000002	No Hit
NGGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGGAGCACA	7	0.17500000000000002	No Hit
NTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTT	7	0.17500000000000002	No Hit
NTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGG	7	0.17500000000000002	No Hit
NTCTTGAACTCTCTCTTCAAAGTCCTTTGCAACTTTCCCTCACGGTACTT	7	0.17500000000000002	No Hit
NTCCTATGCTACGCCGACGGCGTTGGCTGCTCGTTCTAGCCCGACAGTGT	7	0.17500000000000002	No Hit
NCCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGCTTTTCGCCACC	7	0.17500000000000002	No Hit
NACTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCAAGCA	7	0.17500000000000002	No Hit
NCTCTGGCTGAACCAATTCCAGGGAGTCCGTTCCTTAACTAGAGAAGAAA	7	0.17500000000000002	No Hit
NCAGAAAGCGTCCGCAAGCAAGCACAATCACTAGTCCGCCATCAACCCGA	7	0.17500000000000002	No Hit
NTTCCAGGGAGTCCGTTCCTTAACTAGAGAAGAAAACTCTAGCTCGGTCT	7	0.17500000000000002	No Hit
NACAAGTACCGTGAGGGAAAGTTGCAAAGGACTTTGAAGAGAGAGTTCAA	7	0.17500000000000002	No Hit
NTTCGAGACTGACCTTTACTTTCATTTCGCCGCAAGGTTTTAACACCCTT	6	0.15	No Hit
NCGCTAGACAACGCACATGTCGCAACGGCGGAGTATAGGTCTCTCGCTTA	6	0.15	No Hit
NACCAAGGGAGGCTATTAATTTGCACTCAATCGTACCGATATCCGCATTA	6	0.15	No Hit
NCACGGCCCTGTGCGTGTCACTTGTGACTGTGCAGAGGTTGAGCAGTTGG	6	0.15	No Hit
NTATCAACCAACACCTTTTATGGGGTCTCATGAGCGAGAAGTTAGGCACC	6	0.15	No Hit
NTTCTGACGTGCAAATCGATCGATAGACTTGGGTATAGGGGCGAAAGACT	6	0.15	No Hit
NTTCCAACCACTGGAGCCAATCCTTTTCCCGAAGTTACGGATCTAGTTTG	6	0.15	No Hit
NCTTGTGACTGTGCAGAGGTTGAGCAGTTGGCAAACGACCCGAAAGATGG	6	0.15	No Hit
NTCTAGTAGCTGGTTCCTTCCGAAGTTTCCCTCAGGATAGCTGGATCTCA	6	0.15	No Hit
NTGTTGCCCATCTCGCAAGAGAAGGTGTCTTGCTGGCGGTAGTGGGTTCG	6	0.15	No Hit
NCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACC	6	0.15	No Hit
NACTATGCCTGAGCAGGATGAAGCCAGAGGAAACTCTGGTGGAAGTCCGT	6	0.15	No Hit
NTGTTAAAACCTTGCGGCGAAATGAAAGTAAAGGTCAGTCTCGAATTGGC	6	0.15	No Hit
NACCAAGTTCGAATGCGTTACCGCGCTTGTGCTCCGAAGAACACAATCTT	6	0.15	No Hit
NAGTAGACAAGCACTCCGCAATCCGTGTTTCAAGACGGGTCGGAAACGTC	6	0.15	No Hit
NGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCTTCC	6	0.15	No Hit
NTCTCGAATTGGCCGACGTGGGATCTGTGTTCTTCGGAGTGCAGCGCACC	6	0.15	No Hit
NATTAACCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGCTTTTCG	6	0.15	No Hit
NCTTAGCGGATACCGACTTCCATGGCCACCGTCCTGCTGTCAATATCAAC	6	0.15	No Hit
NCGCTAGTGTGTGTGACGTCGGTGTGAAAGTCGACGACGTTTCCGACCCG	6	0.15	No Hit
NGACAAGCATCAACCAACTGGCCAACCGTTCCAACCACTGGAGCCAATCC	6	0.15	No Hit
NTCATAGCCTTCAACGCAACCGCCACGACCTTCCTACTCGTTACGACCTG	6	0.15	No Hit
NTTCTGGATAGCGGCACTGTTGGTTCGCTTGCTCCTTGGAGTCGGGTTGC	6	0.15	No Hit
NTGCTTGTCTACTGCGAGTCAAAGGGTGTTAAAACCTTGCGGCGAAATGA	6	0.15	No Hit
NTCAAAGACCGAGCTAGAGTTTTCTTCTCTAGTTAAGGAACGGACTCCCT	6	0.15	No Hit
NGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGGAGCACAA	6	0.15	No Hit
NTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGC	6	0.15	No Hit
NTTCTGTCTGCTTGATACTTTCGGGTTGATGGCGGACTAGTGATTGTGCT	5	0.125	No Hit
NTACCCAAGTCTATCGATCGATTTGCACGTCAGAACCGATACGGACTTCC	5	0.125	No Hit
NGATGTGGGTCAGTCGATCCTAAGGTACTGGCGAACGCCTTGTATCATCG	5	0.125	No Hit
NGATGAAGCCAGAGGAAACTCTGGTGGAAGTCCGTATCGGTTCTGACGTG	5	0.125	No Hit
NCTCAGGCATAGTTCACCATCTTTCGGGTCGTTTGCCAACTGCTCAACCT	5	0.125	No Hit
NTCTCAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAGCATATCAT	5	0.125	No Hit
NACGTTGACAGTTCACTAAAGAAACTGCAACTTCATATCCATTGAAAGTT	5	0.125	No Hit
NCTATACCCAAGTCTATCGATCGATTTGCACGTCAGAACCGATACGGACT	5	0.125	No Hit
NTTTAGATGAGCGATCAGGATACTAGAACCGAGGTCCAAGCACACACCAG	5	0.125	No Hit
NTCGATCCTAAGGTACTGGCGAACGCCTTGTATCATCGGTGGCGAAAAGC	5	0.125	No Hit
NTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAAGGATCGAACTTGAAT	5	0.125	No Hit
NATATGAAGTTGCAGTTTCTTTAGTGAACTGTCAACGTGAATGCGAGGTC	5	0.125	No Hit
NAATATAACTGCCTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAA	5	0.125	No Hit
NGACACCACAGAAACCGCGGTGCTTTTCGGAAACAACGTCCCCATCTCTG	5	0.125	No Hit
NAGTTAGGGAATATTAACCCTATTCCCTTTCGACAAGCGGGGACTAAAAG	5	0.125	No Hit
NACCATCTGTCACCTCTGTTTTAATTAGACAGTCGGATTCCCTGAGTCCG	5	0.125	No Hit
NCTCTGTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAG	5	0.125	No Hit
NGTGTTGGTTGATATTGACAGCAGGACGGTGGCCATGGAAGTCGGTATCC	5	0.125	No Hit
NGCTATGAGCGTAGGCTCGGCTGGAGCTTCCGTCAGTGCAGATCGTAATG	5	0.125	No Hit
NATGAGACCCCATAAAAGGTGTTGGTTGATATTGACAGCAGGACGGTGGC	5	0.125	No Hit
NATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGAGAC	5	0.125	No Hit
NCGTAATGGTAGTAGCAAATATTCAAGTTCGATCCTTGAAGACTGAAGTG	5	0.125	No Hit
NCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGCTTTTCGCCACCG	5	0.125	No Hit
NTACTGGCGAACGCCTTGTATCATCGGTGGCGAAAAGCTTGCTTTTAGTC	5	0.125	No Hit
NTACTTGTTCGCTATCGCAATCGAGTCGATATTTAGCCTTAGATGAAGTT	5	0.125	No Hit
NCATTACGATCTGCACTGACGGAAGCTCCAGCCGAGCCTACGCTCATAGC	5	0.125	No Hit
NATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGGGAAAAGG	5	0.125	No Hit
NGCAAGCACAATCACTAGTCCGCCATCAACCCGAAAGTATCAAGCAGACA	5	0.125	No Hit
NTACCATTACGATCTGCACTGACGGAAGCTCCAGCCGAGCCTACGCTCAT	5	0.125	No Hit
NAACTAAGCGACCAGTCACCAAGCTTGTCTCCAAGCAACATCAACTGTCT	5	0.125	No Hit
NTTCAAGAGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGTTGATGTT	5	0.125	No Hit
NTTAAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTT	5	0.125	No Hit
NGCTAATGATTAGAGGCCTTGGGGACGTAATGTCCTCAACCTATTCTCAA	5	0.125	No Hit
NTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCAAGGACCATCTGTC	5	0.125	No Hit
NAACTTAAGCATATCATTTAGCGGAGGAAAAGAAACTAAAAAGGATTCCC	5	0.125	No Hit
NTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCTTCCGA	5	0.125	No Hit
NTCTTTGGCTGCTTCGAAATGTGGCGTATAGGTGTAAGTTTCCAGCAGTG	5	0.125	No Hit
NGAATAGGGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGG	5	0.125	No Hit
NAAGTGACACGCACAGGGCCGTGGTGCGCTGCACTCCGAAGAACACAGAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0125	0.0
32-33	0.0	0.0	0.0	0.05	0.0
34-35	0.0	0.0	0.0	0.075	0.0
36-37	0.0	0.0	0.0	0.1125	0.0
38-39	0.0	0.0	0.0	0.2875	0.0
40-41	0.0	0.0	0.0	0.35	0.0
42-43	0.0	0.0	0.0	0.4625	0.0
44-45	0.0	0.0	0.0	0.5375000000000001	0.0
46-47	0.0	0.0	0.0	0.6125	0.0
48-49	0.0	0.0	0.0	0.825	0.0
50-51	0.0	0.0	0.0	1.05	0.0
52-53	0.0	0.0	0.0	1.1875	0.0
54-55	0.0	0.0	0.0	1.225	0.0
56-57	0.0	0.0	0.0	1.3875000000000002	0.0
58-59	0.0	0.0	0.0	1.6	0.0
60-61	0.0	0.0	0.0	1.8125	0.0
62-63	0.0	0.0	0.0	2.0	0.0
64-65	0.0	0.0	0.0	2.1125	0.0
66-67	0.0	0.0	0.0	2.2625	0.0
68-69	0.0	0.0	0.0	2.45	0.0
70-71	0.0	0.0	0.0	2.6125	0.0
72-73	0.0	0.0	0.0	2.7625	0.0
74-75	0.0	0.0	0.0	2.9125	0.0
76-77	0.0	0.0	0.0	3.2125000000000004	0.0
78-79	0.0	0.0	0.0	3.525	0.0
80-81	0.0	0.0	0.0	3.7125	0.0
82-83	0.0	0.0	0.0	3.9625000000000004	0.0
84-85	0.0	0.0	0.0	4.15	0.0
86-87	0.0	0.0	0.0	4.35	0.0
88-89	0.0	0.0	0.0	4.4375	0.0
90-91	0.0	0.0	0.0	4.5875	0.0
92-93	0.0	0.0	0.0	4.675000000000001	0.0
94-95	0.0	0.0	0.0	4.9	0.0
96-97	0.0	0.0	0.0	5.025	0.0
98-99	0.0	0.0	0.0	5.25	0.0
100-101	0.0	0.0	0.0	5.525	0.0
102-103	0.0	0.0	0.0	5.7	0.0
104-105	0.0	0.0	0.0	5.8375	0.0
106-107	0.0	0.0	0.0	6.1375	0.0
108-109	0.0	0.0	0.0	6.387499999999999	0.0
110-111	0.0	0.0	0.0	6.6375	0.0
112-113	0.0	0.0	0.0	7.0375	0.0
114-115	0.0	0.0	0.0	7.4	0.0
116-117	0.0	0.0	0.0	7.6875	0.0
118-119	0.0	0.0	0.0	8.0375	0.0
120-121	0.0	0.0	0.0	8.325	0.0
122-123	0.0	0.0	0.0	8.5375	0.0
124-125	0.0	0.0	0.0	8.675	0.0
126-127	0.0	0.0	0.0	8.899999999999999	0.0
128-129	0.0	0.0	0.0	9.2125	0.0
130-131	0.0	0.0	0.0	9.524999999999999	0.0
132-133	0.0	0.0	0.0	9.8875	0.0
134-135	0.0	0.0	0.0	10.3125	0.0
136-137	0.0	0.0	0.0	10.8875	0.0
138	0.0	0.0	0.0	11.225	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 1262538 spots for SRR13919965.sra
Written 1262538 spots for SRR13919965.sra
Read 1262538 spots for SRR13919965.sra
Written 1262538 spots for SRR13919965.sra
Read 1262538 spots for SRR13919965.sra
Written 1262538 spots for SRR13919965.sra
Read 1262538 spots for SRR13919965.sra
Written 1262538 spots for SRR13919965.sra
Read 1262538 spots for SRR13919965.sra
Written 1262538 spots for SRR13919965.sra
Read 1262538 spots for SRR13919965.sra
Written 1262538 spots for SRR13919965.sra
Read 1262538 spots for SRR13919965.sra
Written 1262538 spots for SRR13919965.sra
Read 1262538 spots for SRR13919965.sra
Written 1262538 spots for SRR13919965.sra
Read 1262538 spots for SRR13919965.sra
Written 1262538 spots for SRR13919965.sra
Read 1262538 spots for SRR13919965.sra
Written 1262538 spots for SRR13919965.sra
Read 1262538 spots for SRR13919965.sra
Written 1262538 spots for SRR13919965.sra
Read 1262538 spots for SRR13919965.sra
Written 1262538 spots for SRR13919965.sra
Read 1262538 spots for SRR13919965.sra
Written 1262538 spots for SRR13919965.sra
Read 1262550 spots for SRR13919965.sra
Written 1262550 spots for SRR13919965.sra
Read 1262538 spots for SRR13919965.sra
Written 1262538 spots for SRR13919965.sra
Read 1262538 spots for SRR13919965.sra
Written 1262538 spots for SRR13919965.sra
SRR ids: ['SRR13919965.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_4fytj_oa
SRR13919965.sra spots: 20200620
blocks: [[1, 1262538], [1262539, 2525076], [2525077, 3787614], [3787615, 5050152], [5050153, 6312690], [6312691, 7575228], [7575229, 8837766], [8837767, 10100304], [10100305, 11362842], [11362843, 12625380], [12625381, 13887918], [13887919, 15150456], [15150457, 16412994], [16412995, 17675532], [17675533, 18938070], [18938071, 20200620]]
SRR13919965 file size 7417552
SRR13919965 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 16 -o SRR13919965 SRR13919965_1.fastq SRR13919965_2.fastq
Input file:	SRR13919965_1.fastq
Paired file:	SRR13919965_2.fastq
trimmed:	SRR13919965-trimmed-pair1.fastq, SRR13919965-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	16
Wed Dec  1 17:49:43 2021 >> started

Wed Dec  1 17:54:44 2021 >> done (301.505s)
20200620 read pairs processed; of these:
   31964 ( 0.16%) short read pairs filtered out after trimming by size control
    9968 ( 0.05%) empty read pairs filtered out after trimming by size control
20158688 (99.79%) read pairs available; of these:
 1113403 ( 5.52%) trimmed read pairs available after processing
19045285 (94.48%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 81	       1	  0.00%
 82	       6	  0.00%
 83	      94	  0.00%
 84	    2562	  0.01%
 85	    3321	  0.02%
 86	    2627	  0.01%
 87	    4561	  0.02%
 88	    3961	  0.02%
 89	    1469	  0.01%
 90	    2833	  0.01%
 91	    2735	  0.01%
 92	    2455	  0.01%
 93	    3553	  0.02%
 94	    1222	  0.01%
 95	    2526	  0.01%
 96	    1344	  0.01%
 97	    2777	  0.01%
 98	    1524	  0.01%
 99	    2434	  0.01%
100	    1841	  0.01%
101	    1235	  0.01%
102	     118	  0.00%
103	     624	  0.00%
104	     907	  0.00%
105	     861	  0.00%
106	     423	  0.00%
107	     688	  0.00%
108	       4	  0.00%
109	     545	  0.00%
110	      59	  0.00%
111	    1016	  0.01%
112	     180	  0.00%
113	       0	  0.00%
114	       0	  0.00%
115	       7	  0.00%
116	       0	  0.00%
117	       8	  0.00%
118	       9	  0.00%
119	     944	  0.00%
120	      42	  0.00%
121	      68	  0.00%
122	      53	  0.00%
123	       0	  0.00%
124	     338	  0.00%
125	      13	  0.00%
126	     114	  0.00%
127	       8	  0.00%
128	      26	  0.00%
129	     485	  0.00%
130	      36	  0.00%
131	     130	  0.00%
132	      22	  0.00%
133	     873	  0.00%
134	      43	  0.00%
135	    2049	  0.01%
136	    1553	  0.01%
137	    1612	  0.01%
138	     866	  0.00%
139	      16	  0.00%
140	     931	  0.00%
141	     124	  0.00%
142	     733	  0.00%
143	     146	  0.00%
144	     511	  0.00%
145	    1463	  0.01%
146	    5937	  0.03%
147	   17982	  0.09%
148	   78639	  0.39%
149	  947116	  4.70%
150	19045285	 94.48%
20158688 reads passed initial QC


criterion=sequence-density
sequence-density=10.15
sequence-density-rank=1
fanout-score=45.87
fanout-score-rank=1
prefix-density=10.66
prefix-fanout=43.6
sequence=CTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA


criterion=fanout-score
sequence-density=10.15
sequence-density-rank=1
fanout-score=45.87
fanout-score-rank=1
prefix-density=10.66
prefix-fanout=43.6
sequence=CTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA


criterion=sequence-density
sequence-density=10.26
sequence-density-rank=1
fanout-score=46.25
fanout-score-rank=1
prefix-density=10.78
prefix-fanout=44.0
sequence=CTGTCTCTTATACACATCTGACGCTGCCGACGAATAGCCTTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAA


criterion=fanout-score
sequence-density=10.26
sequence-density-rank=1
fanout-score=46.25
fanout-score-rank=1
prefix-density=10.78
prefix-fanout=44.0
sequence=CTGTCTCTTATACACATCTGACGCTGCCGACGAATAGCCTTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 818: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 819: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 16 -x CTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA -y CTGTCTCTTATACACATCTGACGCTGCCGACGAATAGCCTTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAA -o SRR13919965 SRR13919965_1.fastq SRR13919965_2.fastq
Input file:	SRR13919965_1.fastq
Paired file:	SRR13919965_2.fastq
trimmed:	SRR13919965-trimmed-pair1.fastq, SRR13919965-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT
-- paired 3' end adapter sequence (-y):	CTGTCTCTTATACACATCTGACGCTGCCGACGAATAGCCTTGTGTAGATCTCGGTGGTCGCCGT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	16
Wed Dec  1 18:08:52 2021 >> started

Wed Dec  1 18:11:44 2021 >> done (172.195s)
16493472 read pairs processed; of these:
      50 ( 0.00%) short read pairs filtered out after trimming by size control
       2 ( 0.00%) empty read pairs filtered out after trimming by size control
16493420 (100.00%) read pairs available; of these:
 2001526 (12.14%) trimmed read pairs available after processing
14491894 (87.86%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       6	  0.00%
 19	      16	  0.00%
 20	      18	  0.00%
 21	      11	  0.00%
 22	      24	  0.00%
 23	      29	  0.00%
 24	      23	  0.00%
 25	      28	  0.00%
 26	      78	  0.00%
 27	     408	  0.00%
 28	     913	  0.01%
 29	    1717	  0.01%
 30	    3011	  0.02%
 31	    3617	  0.02%
 32	    2851	  0.02%
 33	    3202	  0.02%
 34	    3638	  0.02%
 35	    4050	  0.02%
 36	    7559	  0.05%
 37	    8369	  0.05%
 38	    9374	  0.06%
 39	   11212	  0.07%
 40	    8602	  0.05%
 41	    9619	  0.06%
 42	    8161	  0.05%
 43	    8971	  0.05%
 44	    9429	  0.06%
 45	    9516	  0.06%
 46	   10393	  0.06%
 47	   10002	  0.06%
 48	   10640	  0.06%
 49	   10760	  0.07%
 50	   10314	  0.06%
 51	   10344	  0.06%
 52	    9205	  0.06%
 53	   10994	  0.07%
 54	   10217	  0.06%
 55	   11777	  0.07%
 56	   12090	  0.07%
 57	   10500	  0.06%
 58	   11998	  0.07%
 59	   11814	  0.07%
 60	   12156	  0.07%
 61	   12113	  0.07%
 62	   10877	  0.07%
 63	   12102	  0.07%
 64	   11576	  0.07%
 65	   11776	  0.07%
 66	   13194	  0.08%
 67	   12105	  0.07%
 68	   13647	  0.08%
 69	   11574	  0.07%
 70	   13367	  0.08%
 71	   12295	  0.07%
 72	   12862	  0.08%
 73	   12106	  0.07%
 74	   12700	  0.08%
 75	   11840	  0.07%
 76	   13639	  0.08%
 77	   13464	  0.08%
 78	   16790	  0.10%
 79	   12320	  0.07%
 80	   16577	  0.10%
 81	   13266	  0.08%
 82	   15218	  0.09%
 83	   14860	  0.09%
 84	   15447	  0.09%
 85	   17562	  0.11%
 86	   16482	  0.10%
 87	   17663	  0.11%
 88	   18340	  0.11%
 89	   16674	  0.10%
 90	   19697	  0.12%
 91	   19960	  0.12%
 92	   19273	  0.12%
 93	   20986	  0.13%
 94	   16570	  0.10%
 95	   17923	  0.11%
 96	   16874	  0.10%
 97	   19131	  0.12%
 98	   17612	  0.11%
 99	   21046	  0.13%
100	   18647	  0.11%
101	   19273	  0.12%
102	   17818	  0.11%
103	   17277	  0.10%
104	   19571	  0.12%
105	   19618	  0.12%
106	   16714	  0.10%
107	   19855	  0.12%
108	   16825	  0.10%
109	   20133	  0.12%
110	   21700	  0.13%
111	   20786	  0.13%
112	   22193	  0.13%
113	   19132	  0.12%
114	   21275	  0.13%
115	   21408	  0.13%
116	   21447	  0.13%
117	   20224	  0.12%
118	   20583	  0.12%
119	   22801	  0.14%
120	   22698	  0.14%
121	   25565	  0.16%
122	   23982	  0.15%
123	   22712	  0.14%
124	   23050	  0.14%
125	   19915	  0.12%
126	   22720	  0.14%
127	   22005	  0.13%
128	   25061	  0.15%
129	   26589	  0.16%
130	   25438	  0.15%
131	   26527	  0.16%
132	   23286	  0.14%
133	   25190	  0.15%
134	   25429	  0.15%
135	   29043	  0.18%
136	   27240	  0.17%
137	   28445	  0.17%
138	   26157	  0.16%
139	   25482	  0.15%
140	   29100	  0.18%
141	   26911	  0.16%
142	   29691	  0.18%
143	   27175	  0.16%
144	   27105	  0.16%
145	   32720	  0.20%
146	   33595	  0.20%
147	   42712	  0.26%
148	   87878	  0.53%
149	  734054	  4.45%
150	13659501	 82.82%


criterion=sequence-density
sequence-density=4.32
sequence-density-rank=1
fanout-score=2.06
fanout-score-rank=31
prefix-density=4.34
prefix-fanout=2.0
sequence=CTCCGAAGAACACA


criterion=fanout-score
sequence-density=0.05
sequence-density-rank=34
fanout-score=55.51
fanout-score-rank=1
prefix-density=2.68
prefix-fanout=1.0
sequence=GTACATGGGTAGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGCGTTACTTACCACGAATTGCAGACGCTTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTAACTCAATGCCTTAGGCTTCTCTTCGGAGAGTCTTCGGCTTGTCGGGCAACATTAGTGAGCTGAGATTCGCGTCTCGGCATACTGATCTTGTCCTAGTCCAGAAGCATCACAAGTCAAGACAGAGCTTCACCGTTCTGTCTTGTAATGTGACGCGCTACTCTTATATCGGAGGAGCTGCCAAATGGCGTCCGACTTGACTCGGCTGATCATCAAGACGTGTTTAACTGCGTGTCTTATGATGTGAAGCTACTGCATCTATGTCAGAATGTGTCGTCGCTCCTTTCGAATACTGGGATTCGTCTAGTCTCGTGTGTGTGTTGATATCGAATTAATTTTCGATTGATGCGG


criterion=sequence-density
sequence-density=4.05
sequence-density-rank=1
fanout-score=2.07
fanout-score-rank=31
prefix-density=4.08
prefix-fanout=2.1
sequence=CTCCGAAGAACACA


criterion=fanout-score
sequence-density=0.05
sequence-density-rank=33
fanout-score=55.09
fanout-score-rank=1
prefix-density=2.66
prefix-fanout=1.0
sequence=GTACATGGGTAGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGCGTTACTTACCACGAATTGCAGACGCTTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTAACTCAATGCCTTAGGCTTCTCTTCGGAGAGTCTTCGGCTTGTCGGGCAACATTAGTGAGCTGAGATTCGCGTCTCGGCATACTGATCTTGTCCTAGTCCAGAAGCATCACAAGTCAAGACAGAGCTTCACCGTTCTGTCTTGTAATGTGACGCGCTACTCTTATATCGGAGGAGCTGCCAAATGGCGTCCGACTTGACTCGGCTGATCATCAAGACGTGTTTAACTGCGTGTCTTATGATGTGAAGCTACTGCATCTATGTCAGAATGTGTCGTCGCTCCTTTCGAATACTGGGATTCGTCTAGTCTCGTGTGTGTGTTGATATCGAATTAATTTTCGATTGATGCGG
SRR13919965 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Dec 01 18:18:39
                             Started mapping on |	Dec 01 18:18:40
                                    Finished on |	Dec 01 18:27:26
       Mapping speed, Million of reads per hour |	137.97

                          Number of input reads |	20158636
                      Average input read length |	290
                                    UNIQUE READS:
                   Uniquely mapped reads number |	17630514
                        Uniquely mapped reads % |	87.46%
                          Average mapped length |	287.26
                       Number of splices: Total |	2234708
            Number of splices: Annotated (sjdb) |	2135421
                       Number of splices: GT/AG |	2204527
                       Number of splices: GC/AG |	11243
                       Number of splices: AT/AC |	599
               Number of splices: Non-canonical |	18339
                      Mismatch rate per base, % |	0.34%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.41
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.15
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1539775
             % of reads mapped to multiple loci |	7.64%
        Number of reads mapped to too many loci |	19955
             % of reads mapped to too many loci |	0.10%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.72%
                     % of reads unmapped: other |	0.08%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	990105	990105	990105
N_multimapping	1539775	1539775	1539775
N_noFeature	470507	9062806	8923132
N_ambiguous	157587	26329	25917
UnstrandedReadsAssigned:17002420 PositiveStrandReadsAssigned:8541379 NegativeStrandReadsAssigned:8681465
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR13919965 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 33,746
[index] number of k-mers: 28,927,460
[index] number of equivalence classes: 54,013
[quant] running in paired-end mode
[quant] will process pair 1: SRR13919965-trimmed-pair1.fastq
                             SRR13919965-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 20,158,636 reads, 2,836,094 reads pseudoaligned
[quant] estimated average fragment length: 289.092
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,074 rounds

  33747 SRR13919965.ke.tsv
  46739 SRR13919965.se.tsv
  80486 total
==> SRR13919965.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Y110A7A.10	1787	1498.91	292.913	52.2384
F27C8.1	1940	1651.91	97	15.6968
F07C3.7	1728	1439.91	87	16.1514
F52H2.2a	1739	1450.91	80.9037	14.9058
F52H2.2b	1840	1551.91	46.0963	7.94009
T13A10.10a	1734	1445.91	0	0
T13A10.10b	1598	1309.91	3	0.612218
C55C2.5a	1840	1551.91	53.468	9.20987
C55C2.5b	1606	1317.91	13.7068	2.7802

==> SRR13919965.se.tsv <==
WBGene00197333	0
WBGene00198386	0
WBGene00015153	0
WBGene00002061	50
WBGene00255704	22
WBGene00235314	94
WBGene00001177	982
WBGene00169236	0
WBGene00219784	1
WBGene00015152	2
SRR13919965 completed mapping pipeline successfully
