Starting /dee2/code/volunteer_pipeline.sh SRR13919973
    current disk space = 720836460
    free memory = 29554492 
SRR13919973 SRAfilesize
2fad2f4ad3e85035fe1aa87799bab80a  SRR13919973.sra
SRR13919973.sra file validated
SRR13919973 is paired end
SRR13919973 is conventional basespace
SRR13919973 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13919973_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3425	32.0	32.0	32.0	32.0	32.0
2	31.62625	32.0	32.0	32.0	32.0	32.0
3	35.60875	37.0	37.0	37.0	32.0	37.0
4	36.26625	37.0	37.0	37.0	37.0	37.0
5	36.49875	37.0	37.0	37.0	37.0	37.0
6	40.059	41.0	41.0	41.0	37.0	41.0
7	40.21075	41.0	41.0	41.0	37.0	41.0
8	40.07875	41.0	41.0	41.0	37.0	41.0
9	40.16925	41.0	41.0	41.0	37.0	41.0
10-14	40.17620000000001	41.0	41.0	41.0	37.0	41.0
15-19	40.18749999999999	41.0	41.0	41.0	38.6	41.0
20-24	40.08194999999999	41.0	41.0	41.0	37.0	41.0
25-29	40.06085	41.0	41.0	41.0	37.0	41.0
30-34	39.92665	41.0	41.0	41.0	37.0	41.0
35-39	39.823750000000004	41.0	41.0	41.0	37.0	41.0
40-44	39.8861	41.0	41.0	41.0	37.0	41.0
45-49	39.80995	41.0	41.0	41.0	37.0	41.0
50-54	39.737049999999996	41.0	41.0	41.0	37.0	41.0
55-59	39.59595	41.0	41.0	41.0	37.0	41.0
60-64	39.55745	41.0	41.0	41.0	37.0	41.0
65-69	39.559000000000005	41.0	41.0	41.0	37.0	41.0
70-74	39.56505	41.0	41.0	41.0	37.0	41.0
75-79	39.27245	41.0	40.2	41.0	36.0	41.0
80-84	39.5422	41.0	41.0	41.0	37.0	41.0
85-89	39.50940000000001	41.0	41.0	41.0	37.0	41.0
90-94	39.5349	41.0	41.0	41.0	37.0	41.0
95-99	39.6409	41.0	41.0	41.0	37.0	41.0
100-104	39.4045	41.0	41.0	41.0	37.0	41.0
105-109	39.288599999999995	41.0	41.0	41.0	37.0	41.0
110-114	39.2456	41.0	41.0	41.0	37.0	41.0
115-119	39.048449999999995	41.0	41.0	41.0	35.0	41.0
120-124	38.88605	41.0	41.0	41.0	32.0	41.0
125-129	38.597100000000005	41.0	41.0	41.0	33.0	41.0
130-134	38.64014999999999	41.0	41.0	41.0	32.0	41.0
135-139	38.4187	41.0	40.2	41.0	32.0	41.0
140-144	38.23205	41.0	38.6	41.0	32.0	41.0
145-149	37.852	41.0	37.0	41.0	30.0	41.0
150	35.7195	41.0	37.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1201	1	0.0
1201	2	0.0
1201	3	0.0
1201	4	0.0
1201	5	0.0
1201	6	0.0
1201	7	0.0
1201	8	0.0
1201	9	0.0
1201	10-14	0.0
1201	15-19	0.0
1201	20-24	0.0
1201	25-29	0.0
1201	30-34	0.0
1201	35-39	0.0
1201	40-44	0.0
1201	45-49	0.0
1201	50-54	0.0
1201	55-59	0.0
1201	60-64	0.0
1201	65-69	0.0
1201	70-74	0.0
1201	75-79	0.0
1201	80-84	0.0
1201	85-89	0.0
1201	90-94	0.0
1201	95-99	0.0
1201	100-104	0.0
1201	105-109	0.0
1201	110-114	0.0
1201	115-119	0.0
1201	120-124	0.0
1201	125-129	0.0
1201	130-134	0.0
1201	135-139	0.0
1201	140-144	0.0
1201	145-149	0.0
1201	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	4.0
25	6.0
26	15.0
27	16.0
28	15.0
29	25.0
30	30.0
31	32.0
32	49.0
33	56.0
34	97.0
35	96.0
36	144.0
37	163.0
38	225.0
39	466.0
40	2560.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.8936170212766	20.66869300911854	13.728470111448834	25.70921985815603
2	15.925	22.075	38.1	23.9
3	17.0	25.025	30.575000000000003	27.400000000000002
4	13.725000000000001	17.525	37.15	31.6
5	14.374999999999998	37.974999999999994	32.875	14.774999999999999
6	27.125	39.825	17.849999999999998	15.2
7	27.950000000000003	32.95	21.725	17.375
8	25.874999999999996	33.5	22.525000000000002	18.099999999999998
9	26.825	14.325	20.45	38.4
10-14	24.97	24.08	26.669999999999998	24.279999999999998
15-19	24.03	28.075	24.310000000000002	23.585
20-24	24.895	26.275	25.085	23.745
25-29	23.935000000000002	27.544999999999998	24.610000000000003	23.91
30-34	23.445	25.455	26.369999999999997	24.73
35-39	24.959999999999997	25.919999999999998	25.055	24.065
40-44	23.865	25.840000000000003	25.275	25.019999999999996
45-49	24.335	26.085	25.22	24.36
50-54	24.433211550973425	26.90055552775136	24.858615684900656	23.807617236374558
55-59	23.685000000000002	26.565	25.71	24.04
60-64	24.534441329595513	25.96615939126952	25.685822987585098	23.81357629154986
65-69	23.73237323732373	25.54755475547555	25.567556755675568	25.152515251525152
70-74	23.98	24.97	26.505000000000003	24.545
75-79	23.517934864175295	25.899244584521487	26.464555505528043	24.118265045775175
80-84	25.212734007408148	26.51917108819702	23.75613174491941	24.51196315947542
85-89	24.284427542033626	25.160128102481988	26.130904723779025	24.424539631705365
90-94	23.52	25.61	26.43	24.44
95-99	25.047504750475046	24.76247624762476	25.28752875287529	24.902490249024904
100-104	23.981199059953	25.646282314115705	25.056252812640633	25.316265813290666
105-109	23.726186309315466	24.956247812390618	25.876293814690737	25.441272063603183
110-114	23.705000000000002	25.900000000000002	25.485000000000003	24.91
115-119	22.91	26.889999999999997	25.624999999999996	24.575
120-124	23.414365746298518	26.27551020408163	25.765306122448976	24.544817927170868
125-129	23.133506805444355	26.396116893514808	25.625500400320256	24.844875900720574
130-134	23.338670936749402	25.09507606084868	25.840672538030425	25.725580464371493
135-139	23.693955164131307	27.031625300240194	24.989991993594877	24.284427542033626
140-144	22.983386709367494	26.5162129703763	25.430344275420335	25.07005604483587
145-149	23.42310772927513	27.23267921505807	24.539447336804166	24.804765718862633
150	23.74749498997996	25.926853707414832	23.146292585170343	27.17935871743487
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	0.5
6	0.5
7	0.5
8	0.5
9	0.0
10	0.5
11	1.0
12	0.5
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	0.5
23	0.5
24	0.5
25	1.0
26	2.0
27	2.0
28	3.0
29	6.0
30	7.5
31	9.0
32	9.5
33	9.5
34	18.0
35	27.5
36	28.0
37	30.0
38	38.5
39	47.5
40	62.5
41	76.5
42	87.0
43	109.0
44	143.5
45	175.0
46	228.5
47	284.0
48	329.5
49	338.0
50	315.5
51	311.0
52	281.0
53	243.0
54	213.0
55	148.0
56	96.5
57	87.5
58	81.0
59	65.5
60	39.5
61	20.0
62	9.0
63	4.0
64	1.0
65	0.0
66	1.5
67	2.0
68	1.0
69	0.5
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.3
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.095
55-59	0.0
60-64	0.12
65-69	0.01
70-74	0.0
75-79	0.055
80-84	0.11
85-89	0.08
90-94	0.0
95-99	0.01
100-104	0.005
105-109	0.005
110-114	0.0
115-119	0.0
120-124	0.04
125-129	0.08
130-134	0.08
135-139	0.08
140-144	0.08
145-149	0.12
150	0.2
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.3
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.36115569823436	46.949999999999996
2	12.118780096308186	15.1
3	5.056179775280898	9.45
4	2.568218298555377	6.4
5	1.2841091492776886	4.0
6	1.1637239165329054	4.35
7	0.8426966292134831	3.675
8	0.521669341894061	2.6
9	0.2808988764044944	1.575
>10	0.8025682182985553	5.8999999999999995
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTAATGCGGAT	20	0.5	No Hit
TCCAACTACTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAAGGATC	16	0.4	No Hit
CACCAGAAAGCGTCCGCAAGCAAGCACAATCACTAGTCCGCCATCAACCC	14	0.35000000000000003	No Hit
GTATAGGTCTCTCGCTTAAGCGCCATCCATTTTCAGGGCTAGTTGATTCG	13	0.325	No Hit
ATCCTAAGGTACTGGCGAACGCCTTGTATCATCGGTGGCGAAAAGCTTGC	13	0.325	No Hit
GAATAATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGGGAA	12	0.3	No Hit
GTAGTTGGATGTGGGTCAGTCGATCCTAAGGTACTGGCGAACGCCTTGTA	12	0.3	No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTAATGCGGATATCGGTACGATTGA	11	0.27499999999999997	No Hit
GTCTTTGGCTGCTTCGAAATGTGGCGTATAGGTGTAAGTTTCCAGCAGTG	11	0.27499999999999997	No Hit
GTCTAGCGCCAGGTCGTAACGAGTAGGAAGGTCGTGGCGGTTGCGTTGAA	11	0.27499999999999997	No Hit
CCTTGGAGACCTAATGCGGATATCGGTACGATTGAGTGCAAATTAATAGC	11	0.27499999999999997	No Hit
GACCTATACTCCGCCGTTGCGACATGTGCGTTGTCTAGCGCCAGGTCGTA	11	0.27499999999999997	No Hit
CTCCTTAGCGGATACCGACTTCCATGGCCACCGTCCTGCTGTCAATATCA	11	0.27499999999999997	No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCGCT	10	0.25	No Hit
GTTCCAGTACGGTTGTTTAGATGAGCGATCAGGATACTAGAACCGAGGTC	10	0.25	No Hit
TCGTAATGGTAGTAGCAAATATTCAAGTTCGATCCTTGAAGACTGAAGTG	10	0.25	No Hit
GGATACTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCAA	10	0.25	No Hit
CAATCGTACCGATATCCGCATTAGGTCTCCAAGGTGAACAGCCTCTAGTC	10	0.25	No Hit
GGGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGGAGCACA	10	0.25	No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCA	10	0.25	No Hit
GTCTTTGAGCGACTAACCAAGTTCGAATGCGTTACCGCGCTTGTGCTCCG	9	0.22499999999999998	No Hit
TTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGAGACAC	9	0.22499999999999998	No Hit
GTGCAGATCGTAATGGTAGTAGCAAATATTCAAGTTCGATCCTTGAAGAC	9	0.22499999999999998	No Hit
GTTATATTCGGTAAAGCTAATGATTAGAGGCCTTGGGGACGTAATGTCCT	9	0.22499999999999998	No Hit
TATCAACGCAGAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCAA	9	0.22499999999999998	No Hit
TAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGT	9	0.22499999999999998	No Hit
GTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCAAGGACCATCTGTC	9	0.22499999999999998	No Hit
CATGAGACCCCATAAAAGGTGTTGGTTGATATTGACAGCAGGACGGTGGC	8	0.2	No Hit
GTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAAGGATCGAACTTGAAT	8	0.2	No Hit
CACGTTGACAGTTCACTAAAGAAACTGCAACTTCATATCCATTGAAAGTT	8	0.2	No Hit
ACCCGAAAGTATCAAGCAGACAGAACTGCGCCGGACAAGCATCAACCAAC	8	0.2	No Hit
GGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATG	8	0.2	No Hit
ATAGAATAATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGG	8	0.2	No Hit
TACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTAATG	8	0.2	No Hit
ATCAGTGACAGTCAACGTCCGCAAGGACCATCTGTCACCTCTGTTTTAAT	8	0.2	No Hit
GTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGC	8	0.2	No Hit
GTTTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACCGCGGTGCT	8	0.2	No Hit
GTTTAGATGAGCGATCAGGATACTAGAACCGAGGTCCAAGCACACACCAG	8	0.2	No Hit
GGTTGGAACGGTTGGCCAGTTGGTTGATGCTTGTCCGGCGCAGTTCTGTC	8	0.2	No Hit
TACTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCAAGCA	8	0.2	No Hit
CATTAGGTCTCCAAGGTGAACAGCCTCTAGTCGATAGAATAATGTAGGTA	7	0.17500000000000002	No Hit
CCATTAGGACTGACAGATTGAAAGCTCTTTCTCGATTTGGTGGTTGGTGG	7	0.17500000000000002	No Hit
GAATAGGGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGGA	7	0.17500000000000002	No Hit
GTATCATCGGTGGCGAAAAGCTTGCTTTTAGTCCCCGCTTGTCGAAAGGG	7	0.17500000000000002	No Hit
TTCCTATGCTACGCCGACGGCGTTGGCTGCTCGTTCTAGCCCGACAGTGT	7	0.17500000000000002	No Hit
ATATTAACCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGCTTTTC	7	0.17500000000000002	No Hit
TCCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACC	7	0.17500000000000002	No Hit
ACGCAGAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCAAGGACC	7	0.17500000000000002	No Hit
GCCCTTAAAACACCAAGGGAGGCTATTAATTTGCACTCAATCGTACCGAT	7	0.17500000000000002	No Hit
CTTCTCCACTTCAGTCTTCAAGGATCGAACTTGAATATTTGCTACTACCA	7	0.17500000000000002	No Hit
GAACTGCGCCGGACAAGCATCAACCAACTGGCCAACCGTTCCAACCACTG	7	0.17500000000000002	No Hit
GGAATAGGGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGG	7	0.17500000000000002	No Hit
GTCCTCAACCTATTCTCAAACTTTCAATGGATATGAAGTTGCAGTTTCTT	7	0.17500000000000002	No Hit
ATTCCAGGGAGTCCGTTCCTTAACTAGAGAAGAAAACTCTAGCTCGGTCT	7	0.17500000000000002	No Hit
CCACAGAAACCGCGGTGCTTTTCGGAAACAACGTCCCCATCTCTGGCTGA	7	0.17500000000000002	No Hit
AATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGAGAC	7	0.17500000000000002	No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAAT	7	0.17500000000000002	No Hit
ATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGGGAAAAGGA	7	0.17500000000000002	No Hit
GCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAACTAGATC	7	0.17500000000000002	No Hit
CCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGCGATCAGGA	7	0.17500000000000002	No Hit
CTCAAAGACCGAGCTAGAGTTTTCTTCTCTAGTTAAGGAACGGACTCCCT	7	0.17500000000000002	No Hit
ATACTCCGCCGTTGCGACATGTGCGTTGTCTAGCGCCAGGTCGTAACGAG	6	0.15	No Hit
GTTCTGACGTGCAAATCGATCGATAGACTTGGGTATAGGGGCGAAAGACT	6	0.15	No Hit
CCTTAACTCCACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAA	6	0.15	No Hit
GATCTAGTTTGCCGACTTCCCTTACCTACATTATTCTATCGACTAGAGGC	6	0.15	No Hit
GAAGAACACAATCTTTGCATCTCAGTTAGGGAATATTAACCCTATTCCCT	6	0.15	No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGC	6	0.15	No Hit
GGGAATAGGGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCG	6	0.15	No Hit
GGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGGAGCACAA	6	0.15	No Hit
CCTAATGCGGATATCGGTACGATTGAGTGCAAATTAATAGCCTCCCTTGG	6	0.15	No Hit
TATTAACCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGCTTTTCG	6	0.15	No Hit
GGTTGATATTGACAGCAGGACGGTGGCCATGGAAGTCGGTATCCGCTAAG	6	0.15	No Hit
GTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGG	6	0.15	No Hit
TCTCTGGCTGAACCAATTCCAGGGAGTCCGTTCCTTAACTAGAGAAGAAA	6	0.15	No Hit
CCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGCTTTTCGCCACCG	6	0.15	No Hit
GAACACAATCTTTGCATCTCAGTTAGGGAATATTAACCCTATTCCCTTTC	6	0.15	No Hit
CTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCAAGCACA	6	0.15	No Hit
AACCAAGTTCGAATGCGTTACCGCGCTTGTGCTCCGAAGAACACAATCTT	6	0.15	No Hit
GTACTGGCGAACGCCTTGTATCATCGGTGGCGAAAAGCTTGCTTTTAGTC	6	0.15	No Hit
CCCTGGAATTGGTTCAGCCAGAGATGGGGACGTTGTTTCCGAAAAGCACC	6	0.15	No Hit
CCAGAAAGCGTCCGCAAGCAAGCACAATCACTAGTCCGCCATCAACCCGA	6	0.15	No Hit
GAATATAACTGCCTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAA	6	0.15	No Hit
CCTTAGGATCGACTGACCCACATCCAACTACTGTTCACGTGGAACCCTTC	6	0.15	No Hit
GCCAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGGTTTCTGTGGTGT	6	0.15	No Hit
GTCTCGAATTGGCCGACGTGGGATCTGTGTTCTTCGGAGTGCAGCGCACC	6	0.15	No Hit
ACCCAAGTCTATCGATCGATTTGCACGTCAGAACCGATACGGACTTCCAC	6	0.15	No Hit
GTTCAAAGAGCACGAGACACCACAGAAACCGCGGTGCTTTTCGGAAACAA	6	0.15	No Hit
CCTTTTATGGGGTCTCATGAGCGAGAAGTTAGGCACCTTAACTCCACGTT	6	0.15	No Hit
GGATATGAAGTTGCAGTTTCTTTAGTGAACTGTCAACGTGAATGCGAGGT	6	0.15	No Hit
ATTCGAGACTGACCTTTACTTTCATTTCGCCGCAAGGTTTTAACACCCTT	6	0.15	No Hit
CTCTAGCTCGGTCTTTGAGCGACTAACCAAGTTCGAATGCGTTACCGCGC	5	0.125	No Hit
AGCAAGCACAATCACTAGTCCGCCATCAACCCGAAAGTATCAAGCAGACA	5	0.125	No Hit
CACTAGTCCGCCATCAACCCGAAAGTATCAAGCAGACAGAACTGCGCCGG	5	0.125	No Hit
CAACCGTTCCAACCACTGGAGCCAATCCTTTTCCCGAAGTTACGGATCTA	5	0.125	No Hit
GTTCCAACCACTGGAGCCAATCCTTTTCCCGAAGTTACGGATCTAGTTTG	5	0.125	No Hit
CACCAAGGGAGGCTATTAATTTGCACTCAATCGTACCGATATCCGCATTA	5	0.125	No Hit
ACTAAACGCTAGCCGCCACGAACCCACTACCGCCAGCAAGACACCTTCTC	5	0.125	No Hit
CTTAAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTT	5	0.125	No Hit
AGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGGGAAAAGGATTGG	5	0.125	No Hit
CGCAAGGACCATCTGTCACCTCTGTTTTAATTAGACAGTCGGATTCCCTG	5	0.125	No Hit
AGACAGAACTGCGCCGGACAAGCATCAACCAACTGGCCAACCGTTCCAAC	5	0.125	No Hit
CCCCTATACCCAAGTCTATCGATCGATTTGCACGTCAGAACCGATACGGA	5	0.125	No Hit
TTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACCGCGGTGCTTT	5	0.125	No Hit
AAACCAGAGAGGGTGCGAGCCCCGTTCTGGATAGCGGCACTGTTGGTTCG	5	0.125	No Hit
GTGCAGAGGTTGAGCAGTTGGCAAACGACCCGAAAGATGGTGAACTATGC	5	0.125	No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCTATCGAT	5	0.125	No Hit
CCCCATCTCTGGCTGAACCAATTCCAGGGAGTCCGTTCCTTAACTAGAGA	5	0.125	No Hit
GTTCAAGAGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGTTGATGTT	5	0.125	No Hit
GCACAGGGCCGTGGTGCGCTGCACTCCGAAGAACACAGATCCCACGTCGG	5	0.125	No Hit
TCTGTCACCTCTGTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCG	5	0.125	No Hit
CAGTGACAGTCAACGTCCGCAAGGACCATCTGTCACCTCTGTTTTAATTA	5	0.125	No Hit
CCCTAACTGAGATGCAAAGATTGTGTTCTTCGGAGCACAAGCGCGGTAAC	5	0.125	No Hit
CTAGTATCCTGATCGCTCATCTAAACAACCGTACTGGAACCGGTACGGAC	5	0.125	No Hit
CCTCTGCACAGTCACAAGTGACACGCACAGGGCCGTGGTGCGCTGCACTC	5	0.125	No Hit
GACAGAACTGCGCCGGACAAGCATCAACCAACTGGCCAACCGTTCCAACC	5	0.125	No Hit
CAAGCAGACAGAACTGCGCCGGACAAGCATCAACCAACTGGCCAACCGTT	5	0.125	No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCG	5	0.125	No Hit
GTTGTTTAGATGAGCGATCAGGATACTAGAACCGAGGTCCAAGCACACAC	5	0.125	No Hit
CTAAAAAGGATTCCCTTAGTAACGGCGAGTGAAACGGGAAGAGCCCAGCG	5	0.125	No Hit
AGACACCACAGAAACCGCGGTGCTTTTCGGAAACAACGTCCCCATCTCTG	5	0.125	No Hit
CATAAAAGGTGTTGGTTGATATTGACAGCAGGACGGTGGCCATGGAAGTC	5	0.125	No Hit
AGCGTAACTAAACGCTAGCCGCCACGAACCCACTACCGCCAGCAAGACAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0125	0.0
30-31	0.0	0.0	0.0	0.025	0.0
32-33	0.0	0.0	0.0	0.05	0.0
34-35	0.0	0.0	0.0	0.0875	0.0
36-37	0.0	0.0	0.0	0.1125	0.0
38-39	0.0	0.0	0.0	0.26249999999999996	0.0
40-41	0.0	0.0	0.0	0.4	0.0
42-43	0.0	0.0	0.0	0.575	0.0
44-45	0.0	0.0	0.0	0.675	0.0
46-47	0.0	0.0	0.0	0.8375	0.0
48-49	0.0	0.0	0.0	1.0	0.0
50-51	0.0	0.0	0.0	1.1625	0.0
52-53	0.0	0.0	0.0	1.3	0.0
54-55	0.0	0.0	0.0	1.3625	0.0
56-57	0.0	0.0	0.0	1.4625	0.0
58-59	0.0	0.0	0.0	1.5875	0.0
60-61	0.0	0.0	0.0	1.725	0.0
62-63	0.0	0.0	0.0	1.9375	0.0
64-65	0.0	0.0	0.0	2.0	0.0
66-67	0.0	0.0	0.0	2.1875	0.0
68-69	0.0	0.0	0.0	2.3625	0.0
70-71	0.0	0.0	0.0	2.575	0.0
72-73	0.0	0.0	0.0	2.7375	0.0
74-75	0.0	0.0	0.0	2.875	0.0
76-77	0.0	0.0	0.0	2.9749999999999996	0.0
78-79	0.0	0.0	0.0	3.175	0.0
80-81	0.0	0.0	0.0	3.3375	0.0
82-83	0.0	0.0	0.0	3.4375	0.0
84-85	0.0	0.0	0.0	3.5625	0.0
86-87	0.0	0.0	0.0	3.725	0.0
88-89	0.0	0.0	0.0	3.9125	0.0
90-91	0.0	0.0	0.0	4.1375	0.0
92-93	0.0	0.0	0.0	4.387499999999999	0.0
94-95	0.0	0.0	0.0	4.6375	0.0
96-97	0.0	0.0	0.0	4.675000000000001	0.0
98-99	0.0	0.0	0.0	4.9375	0.0
100-101	0.0	0.0	0.0	5.237500000000001	0.0
102-103	0.0	0.0	0.0	5.4125	0.0
104-105	0.0	0.0	0.0	5.5375	0.0
106-107	0.0	0.0	0.0	5.825	0.0
108-109	0.0	0.0	0.0	6.1	0.0
110-111	0.0	0.0	0.0	6.262499999999999	0.0
112-113	0.0	0.0	0.0	6.5625	0.0
114-115	0.0	0.0	0.0	6.825	0.0
116-117	0.0	0.0	0.0	7.125	0.0
118-119	0.0	0.0	0.0	7.4375	0.0
120-121	0.0	0.0	0.0	7.875	0.0
122-123	0.0	0.0	0.0	8.325	0.0
124-125	0.0	0.0	0.0	8.575	0.0
126-127	0.0	0.0	0.0	8.875	0.0
128-129	0.0	0.0	0.0	9.1375	0.0
130-131	0.0	0.0	0.0	9.3875	0.0
132-133	0.0	0.0	0.0	9.837499999999999	0.0
134-135	0.0	0.0	0.0	10.225000000000001	0.0
136-137	0.0	0.0	0.0	10.425	0.0
138	0.0	0.0	0.0	10.6	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCAG	10	0.006991776	143.875	6
GCTGCTT	10	0.006991776	143.875	8
CTGCTTC	10	0.006991776	143.875	9
CTTTGGC	10	0.006991776	143.875	3
TCTTTGG	10	0.006991776	143.875	2
AAAAAAA	190	0.0023531285	7.572368	130-134
>>END_MODULE
SRR13919973 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13919973_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	2.0	2.0	2.0	2.0	2.0	2.0
2	31.46375	32.0	32.0	32.0	32.0	32.0
3	31.53125	32.0	32.0	32.0	32.0	32.0
4	35.65125	37.0	37.0	37.0	32.0	37.0
5	36.1825	37.0	37.0	37.0	37.0	37.0
6	39.826	41.0	41.0	41.0	37.0	41.0
7	39.835	41.0	41.0	41.0	37.0	41.0
8	40.091	41.0	41.0	41.0	41.0	41.0
9	40.068	41.0	41.0	41.0	41.0	41.0
10-14	39.9199	41.0	41.0	41.0	38.6	41.0
15-19	40.05165	41.0	41.0	41.0	41.0	41.0
20-24	40.01604999999999	41.0	41.0	41.0	40.2	41.0
25-29	39.9931	41.0	41.0	41.0	41.0	41.0
30-34	39.954499999999996	41.0	41.0	41.0	39.4	41.0
35-39	39.90835	41.0	41.0	41.0	37.8	41.0
40-44	39.887299999999996	41.0	41.0	41.0	38.6	41.0
45-49	39.78275	41.0	41.0	41.0	37.0	41.0
50-54	39.7547	41.0	41.0	41.0	37.0	41.0
55-59	39.818799999999996	41.0	41.0	41.0	37.0	41.0
60-64	39.74015	41.0	41.0	41.0	37.0	41.0
65-69	39.665800000000004	41.0	41.0	41.0	37.0	41.0
70-74	39.5237	41.0	41.0	41.0	37.0	41.0
75-79	39.1322	41.0	40.2	41.0	36.0	41.0
80-84	39.7789	41.0	41.0	41.0	37.0	41.0
85-89	39.7309	41.0	41.0	41.0	37.0	41.0
90-94	39.67395	41.0	41.0	41.0	37.0	41.0
95-99	39.5878	41.0	41.0	41.0	37.0	41.0
100-104	39.501	41.0	41.0	41.0	37.0	41.0
105-109	39.39375	41.0	41.0	41.0	37.0	41.0
110-114	39.31365	41.0	41.0	41.0	37.0	41.0
115-119	39.275999999999996	41.0	41.0	41.0	37.0	41.0
120-124	38.9949	41.0	41.0	41.0	36.0	41.0
125-129	38.637299999999996	41.0	41.0	41.0	32.0	41.0
130-134	38.43755	41.0	41.0	41.0	32.0	41.0
135-139	38.40990000000001	41.0	41.0	41.0	32.0	41.0
140-144	38.13765	41.0	40.2	41.0	31.0	41.0
145-149	37.78165	41.0	37.8	41.0	28.0	41.0
150	31.9305	37.0	27.0	41.0	12.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1201	1	0.0
1201	2	0.0
1201	3	0.0
1201	4	0.0
1201	5	0.0
1201	6	0.0
1201	7	0.0
1201	8	0.0
1201	9	0.0
1201	10-14	0.0
1201	15-19	0.0
1201	20-24	0.0
1201	25-29	0.0
1201	30-34	0.0
1201	35-39	0.0
1201	40-44	0.0
1201	45-49	0.0
1201	50-54	0.0
1201	55-59	0.0
1201	60-64	0.0
1201	65-69	0.0
1201	70-74	0.0
1201	75-79	0.0
1201	80-84	0.0
1201	85-89	0.0
1201	90-94	0.0
1201	95-99	0.0
1201	100-104	0.0
1201	105-109	0.0
1201	110-114	0.0
1201	115-119	0.0
1201	120-124	0.0
1201	125-129	0.0
1201	130-134	0.0
1201	135-139	0.0
1201	140-144	0.0
1201	145-149	0.0
1201	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	10.0
15	1.0
16	1.0
17	9.0
18	4.0
19	4.0
20	6.0
21	8.0
22	5.0
23	8.0
24	8.0
25	8.0
26	13.0
27	12.0
28	15.0
29	19.0
30	21.0
31	20.0
32	33.0
33	52.0
34	64.0
35	62.0
36	109.0
37	133.0
38	248.0
39	591.0
40	2532.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	NaN	NaN	NaN	NaN
2	15.7	20.275000000000002	39.65	24.375
3	15.975	26.85	31.075000000000003	26.1
4	13.55	17.724999999999998	38.224999999999994	30.5
5	13.678419604901226	37.43435858964741	33.908477119279816	14.978744686171543
6	29.725	38.775	16.975	14.524999999999999
7	27.593984962406015	32.75689223057644	22.080200501253135	17.56892230576441
8	24.7	33.425	22.625	19.25
9	28.549999999999997	14.424999999999999	19.775000000000002	37.25
10-14	25.12256128064032	23.461730865432717	27.808904452226113	23.60680340170085
15-19	23.76688344172086	27.943971985993	25.362681340670335	22.926463231615806
20-24	25.067533766883443	26.21310655327664	25.62281140570285	23.096548274137067
25-29	23.7987987987988	27.017017017017015	24.824824824824827	24.35935935935936
30-34	24.682213992593336	25.537984185767193	26.023421078971076	23.7563807426684
35-39	24.63216895205685	26.20858772895606	24.997497747973178	24.161745571013913
40-44	24.22179961965769	25.808227404664198	25.29776799119207	24.672204984486036
45-49	25.112601341207085	25.74817335602042	25.3528175357822	23.78640776699029
50-54	24.679615538646377	27.002402883460153	25.0200240288346	23.29795754905887
55-59	23.848618342010415	27.127553063676412	25.145174209050865	23.878654385262315
60-64	24.504405286343612	26.401682018422107	25.851021225470568	23.242891469763716
65-69	24.429315178213855	26.06127352823388	24.61453744493392	24.894873848618342
70-74	24.263822115384613	24.814703525641026	27.223557692307693	23.697916666666664
75-79	23.537660256410255	26.171875	26.717748397435898	23.572716346153847
80-84	25.125200320512818	25.87139423076923	25.015024038461537	23.98838141025641
85-89	24.64443108974359	26.026642628205128	25.5859375	23.742988782051285
90-94	23.847695390781563	26.182364729458918	25.470941883767534	24.498997995991985
95-99	25.44589178356713	24.96993987975952	24.934869739478955	24.649298597194388
100-104	23.873197115384613	25.5859375	25.615985576923077	24.924879807692307
105-109	24.714428857715433	24.96492985971944	25.586172344689377	24.73446893787575
110-114	23.763020833333336	26.231971153846157	25.23036858974359	24.774639423076923
115-119	23.532064128256515	27.149298597194388	25.24048096192385	24.07815631262525
120-124	24.674479166666664	26.392227564102566	24.704527243589745	24.228766025641026
125-129	24.093549679487182	26.637620192307693	25.270432692307693	23.998397435897438
130-134	24.444110576923077	25.856370192307693	25.455729166666668	24.243790064102562
135-139	23.5756483428457	27.821167517773105	24.677080204265543	23.92610393511565
140-144	23.48788303625075	26.286801522130983	26.04145804125776	24.183857400360502
145-149	23.97897897897898	27.52752752752753	24.234234234234233	24.25925925925926
150	24.06015037593985	26.265664160401002	24.43609022556391	25.238095238095237
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	0.5
19	0.0
20	0.0
21	0.5
22	1.0
23	2.0
24	2.5
25	3.5
26	5.5
27	7.0
28	9.5
29	7.5
30	6.0
31	10.0
32	17.0
33	21.5
34	23.0
35	26.5
36	30.0
37	46.0
38	51.5
39	44.5
40	57.5
41	75.5
42	97.5
43	123.5
44	174.5
45	237.0
46	264.5
47	332.0
48	348.5
49	324.5
50	318.0
51	279.0
52	252.5
53	230.5
54	160.0
55	89.0
56	78.5
57	86.5
58	73.0
59	37.0
60	20.0
61	10.5
62	5.0
63	1.5
64	1.0
65	1.0
66	2.0
67	2.5
68	1.0
69	0.5
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	100.0
2	0.0
3	0.0
4	0.0
5	0.025
6	0.0
7	0.25
8	0.0
9	0.0
10-14	0.05
15-19	0.05
20-24	0.05
25-29	0.1
30-34	0.09
35-39	0.09
40-44	0.09
45-49	0.09
50-54	0.12
55-59	0.12
60-64	0.12
65-69	0.12
70-74	0.16
75-79	0.16
80-84	0.16
85-89	0.16
90-94	0.2
95-99	0.2
100-104	0.16
105-109	0.2
110-114	0.16
115-119	0.2
120-124	0.16
125-129	0.16
130-134	0.16
135-139	0.13
140-144	0.13999999999999999
145-149	0.1
150	0.25
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.9
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.58982511923689	48.175000000000004
2	11.327503974562799	14.249999999999998
3	4.769475357710652	9.0
4	2.5039745627980925	6.3
5	1.2718600953895072	4.0
6	1.232114467408585	4.65
7	0.834658187599364	3.675
8	0.3577106518282989	1.7999999999999998
9	0.3179650238473768	1.7999999999999998
>10	0.7949125596184419	6.35
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NCATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTAATGCGGAT	17	0.42500000000000004	No Hit
NTATCAACGCAGAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCA	16	0.4	No Hit
NCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAAT	15	0.375	No Hit
NACCTATACTCCGCCGTTGCGACATGTGCGTTGTCTAGCGCCAGGTCGTA	15	0.375	No Hit
NCCAACTACTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAAGGATC	14	0.35000000000000003	No Hit
NTATAGGTCTCTCGCTTAAGCGCCATCCATTTTCAGGGCTAGTTGATTCG	14	0.35000000000000003	No Hit
NGATACTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCAA	14	0.35000000000000003	No Hit
NACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTAATG	13	0.325	No Hit
NGGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGGAGCACA	13	0.325	No Hit
NTCAAAGACCGAGCTAGAGTTTTCTTCTCTAGTTAAGGAACGGACTCCCT	13	0.325	No Hit
NCTAGAGGCTGTTCACCTTGGAGACCTAATGCGGATATCGGTACGATTGA	13	0.325	No Hit
NTAGTTGGATGTGGGTCAGTCGATCCTAAGGTACTGGCGAACGCCTTGTA	12	0.3	No Hit
NCCTAACTGAGATGCAAAGATTGTGTTCTTCGGAGCACAAGCGCGGTAAC	12	0.3	No Hit
NTCCTAAGGTACTGGCGAACGCCTTGTATCATCGGTGGCGAAAAGCTTGC	11	0.27499999999999997	No Hit
NTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGG	11	0.27499999999999997	No Hit
NTATTAACCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGCTTTTC	11	0.27499999999999997	No Hit
NTCTTTGAGCGACTAACCAAGTTCGAATGCGTTACCGCGCTTGTGCTCCG	10	0.25	No Hit
NTCCTTAGCGGATACCGACTTCCATGGCCACCGTCCTGCTGTCAATATCA	10	0.25	No Hit
NAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGT	10	0.25	No Hit
NTCCTCAACCTATTCTCAAACTTTCAATGGATATGAAGTTGCAGTTTCTT	10	0.25	No Hit
NCTGTCACCTCTGTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCG	9	0.22499999999999998	No Hit
NTCTAGCGCCAGGTCGTAACGAGTAGGAAGGTCGTGGCGGTTGCGTTGAA	9	0.22499999999999998	No Hit
NATTTGCACTCAATCGTACCGATATCCGCATTAGGTCTCCAAGGTGAACA	9	0.22499999999999998	No Hit
NATTAACCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGCTTTTCG	9	0.22499999999999998	No Hit
NTTAAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTT	9	0.22499999999999998	No Hit
NCCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACC	9	0.22499999999999998	No Hit
NTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCAAGGACCATCTGTC	9	0.22499999999999998	No Hit
NAATAATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGGGAA	9	0.22499999999999998	No Hit
NACCAGAAAGCGTCCGCAAGCAAGCACAATCACTAGTCCGCCATCAACCC	8	0.2	No Hit
NTTATATTCGGTAAAGCTAATGATTAGAGGCCTTGGGGACGTAATGTCCT	8	0.2	No Hit
NTTTAGATGAGCGATCAGGATACTAGAACCGAGGTCCAAGCACACACCAG	8	0.2	No Hit
NCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACC	8	0.2	No Hit
NTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACCGCGGTGCTTT	8	0.2	No Hit
NATCAACGCAGAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCAA	8	0.2	No Hit
NCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCT	8	0.2	No Hit
NTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTAATGCGGATATC	8	0.2	No Hit
NTTCCAGTACGGTTGTTTAGATGAGCGATCAGGATACTAGAACCGAGGTC	8	0.2	No Hit
NTTCCAACCACTGGAGCCAATCCTTTTCCCGAAGTTACGGATCTAGTTTG	7	0.17500000000000002	No Hit
NTTTCTGTGGTGTCTCGTGCTCTTTGAACGGCCCTTAAAACACCAAGGGA	7	0.17500000000000002	No Hit
NCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAA	7	0.17500000000000002	No Hit
NCTTGTGACTGTGCAGAGGTTGAGCAGTTGGCAAACGACCCGAAAGATGG	7	0.17500000000000002	No Hit
NCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAACTAGATC	7	0.17500000000000002	No Hit
NACCATCTGTCACCTCTGTTTTAATTAGACAGTCGGATTCCCTGAGTCCG	7	0.17500000000000002	No Hit
NTTTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACCGCGGTGCT	7	0.17500000000000002	No Hit
NCACAGAAACCGCGGTGCTTTTCGGAAACAACGTCCCCATCTCTGGCTGA	7	0.17500000000000002	No Hit
NCTCTGCACAGTCACAAGTGACACGCACAGGGCCGTGGTGCGCTGCACTC	7	0.17500000000000002	No Hit
NGGTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCG	7	0.17500000000000002	No Hit
NCGTAATGGTAGTAGCAAATATTCAAGTTCGATCCTTGAAGACTGAAGTG	7	0.17500000000000002	No Hit
NCATTACGATCTGCACTGACGGAAGCTCCAGCCGAGCCTACGCTCATAGC	7	0.17500000000000002	No Hit
NTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGAGACAC	7	0.17500000000000002	No Hit
NTCCTATGCTACGCCGACGGCGTTGGCTGCTCGTTCTAGCCCGACAGTGT	7	0.17500000000000002	No Hit
NCATAAAAGGTGTTGGTTGATATTGACAGCAGGACGGTGGCCATGGAAGT	7	0.17500000000000002	No Hit
NACTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCAAGCA	7	0.17500000000000002	No Hit
NGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGGAGCACAA	7	0.17500000000000002	No Hit
NCTCTGGCTGAACCAATTCCAGGGAGTCCGTTCCTTAACTAGAGAAGAAA	7	0.17500000000000002	No Hit
NTTCCAGGGAGTCCGTTCCTTAACTAGAGAAGAAAACTCTAGCTCGGTCT	7	0.17500000000000002	No Hit
NTGGTGTCTCGTGCTCTTTGAACGGCCCTTAAAACACCAAGGGAGGCTAT	7	0.17500000000000002	No Hit
NTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGC	7	0.17500000000000002	No Hit
NTGTTCTTCGGAGCACAAGCGCGGTAACGCATTCGAACTTGGTTAGTCGC	6	0.15	No Hit
NGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGGGAAAAGGATTGGC	6	0.15	No Hit
NACCAAGGGAGGCTATTAATTTGCACTCAATCGTACCGATATCCGCATTA	6	0.15	No Hit
NTTCTGACGTGCAAATCGATCGATAGACTTGGGTATAGGGGCGAAAGACT	6	0.15	No Hit
NATATGAAGTTGCAGTTTCTTTAGTGAACTGTCAACGTGAATGCGAGGTC	6	0.15	No Hit
NTATCATCGGTGGCGAAAAGCTTGCTTTTAGTCCCCGCTTGTCGAAAGGG	6	0.15	No Hit
NTATTAATTTGCACTCAATCGTACCGATATCCGCATTAGGTCTCCAAGGT	6	0.15	No Hit
NCTTAACTAGAGAAGAAAACTCTAGCTCGGTCTTTGAGCGACTAACCAAG	6	0.15	No Hit
NAATATAACTGCCTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAA	6	0.15	No Hit
NCTATACTCCGCCGTTGCGACATGTGCGTTGTCTAGCGCCAGGTCGTAAC	6	0.15	No Hit
NCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGCGATCAGGA	6	0.15	No Hit
NATAAAAGGTGTTGGTTGATATTGACAGCAGGACGGTGGCCATGGAAGTC	6	0.15	No Hit
NGTGTGTGTGACGTCGGTGTGAAAGTCGACGACGTTTCCGACCCGTCTTG	6	0.15	No Hit
NTAGAATAATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGG	6	0.15	No Hit
NGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCTTCC	6	0.15	No Hit
NTTGTTTAGATGAGCGATCAGGATACTAGAACCGAGGTCCAAGCACACAC	6	0.15	No Hit
NTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCAAGCACA	6	0.15	No Hit
NTTAAGGAACGGACTCCCTGGAATTGGTTCAGCCAGAGATGGGGACGTTG	6	0.15	No Hit
NGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGGGAAAAGGATTGG	6	0.15	No Hit
NCTTAGCGGATACCGACTTCCATGGCCACCGTCCTGCTGTCAATATCAAC	6	0.15	No Hit
NCTTTTATGGGGTCTCATGAGCGAGAAGTTAGGCACCTTAACTCCACGTT	6	0.15	No Hit
NCGCTAGTGTGTGTGACGTCGGTGTGAAAGTCGACGACGTTTCCGACCCG	6	0.15	No Hit
NTGCAGAGGTTGAGCAGTTGGCAAACGACCCGAAAGATGGTGAACTATGC	6	0.15	No Hit
NTACTGGCGAACGCCTTGTATCATCGGTGGCGAAAAGCTTGCTTTTAGTC	6	0.15	No Hit
NTGCTTGTCTACTGCGAGTCAAAGGGTGTTAAAACCTTGCGGCGAAATGA	6	0.15	No Hit
NTACAAGGCGTTCGCCAGTACCTTAGGATCGACTGACCCACATCCAACTA	6	0.15	No Hit
NCAGAAAGCGTCCGCAAGCAAGCACAATCACTAGTCCGCCATCAACCCGA	6	0.15	No Hit
NTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCTTCCGA	6	0.15	No Hit
NAATCGTACCGATATCCGCATTAGGTCTCCAAGGTGAACAGCCTCTAGTC	6	0.15	No Hit
NGAATAGGGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGG	6	0.15	No Hit
NTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTA	6	0.15	No Hit
NCTTAACTCCACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAA	5	0.125	No Hit
NCACGGCCCTGTGCGTGTCACTTGTGACTGTGCAGAGGTTGAGCAGTTGG	5	0.125	No Hit
NGTTACGACCTGGCGCTAGACAACGCACATGTCGCAACGGCGGAGTATAG	5	0.125	No Hit
NTCCAGAACGGGGCTCGCACCCTCTCTGGTTTATGGCCTCAATCGTAAGG	5	0.125	No Hit
NCTTAGGATCGACTGACCCACATCCAACTACTGTTCACGTGGAACCCTTC	5	0.125	No Hit
NCTAATGCGGATATCGGTACGATTGAGTGCAAATTAATAGCCTCCCTTGG	5	0.125	No Hit
NGTCAGTGCAGATCGTAATGGTAGTAGCAAATATTCAAGTTCGATCCTTG	5	0.125	No Hit
NTGTTACACACTCCTTAGCGGATACCGACTTCCATGGCCACCGTCCTGCT	5	0.125	No Hit
NAAGTGGAGAAGGGTTCCACGTGAACAGTAGTTGGATGTGGGTCAGTCGA	5	0.125	No Hit
NCCCAAGTCTATCGATCGATTTGCACGTCAGAACCGATACGGACTTCCAC	5	0.125	No Hit
NACTAGATCCGTAACTTCGGGAAAAGGATTGGCTCCAGTGGTTGGAACGG	5	0.125	No Hit
NATTACGTCCCCAAGGCCTCTAATCATTAGCTTTACCGAATATAACTGCC	5	0.125	No Hit
NATGAGACCCCATAAAAGGTGTTGGTTGATATTGACAGCAGGACGGTGGC	5	0.125	No Hit
NTCTTGAAACACGGATTGCGGAGTGCTTGTCTACTGCGAGTCAAAGGGTG	5	0.125	No Hit
NTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTT	5	0.125	No Hit
NATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGCGATC	5	0.125	No Hit
NAATGGTAGTAGCAAATATTCAAGTTCGATCCTTGAAGACTGAAGTGGAG	5	0.125	No Hit
NTACCGATATCCGCATTAGGTCTCCAAGGTGAACAGCCTCTAGTCGATAG	5	0.125	No Hit
NTCAACGTGAATGCGAGGTCCAAGTGGGCCATTTTTGGTAAGCAGAACTG	5	0.125	No Hit
NCCTTGTATCATCGGTGGCGAAAAGCTTGCTTTTAGTCCCCGCTTGTCGA	5	0.125	No Hit
NTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCTATCGAT	5	0.125	No Hit
NGCCAGTACCTTAGGATCGACTGACCCACATCCAACTACTGTTCACGTGG	5	0.125	No Hit
NGTTAAGGAACGGACTCCCTGGAATTGGTTCAGCCAGAGATGGGGACGTT	5	0.125	No Hit
NATTAGAACAGACCAAACGTTTTCGGACGTTGTTTGTTGACTCTGAATAA	5	0.125	No Hit
NTGGAGAAGGGTTCCACGTGAACAGTAGTTGGATGTGGGTCAGTCGATCC	5	0.125	No Hit
NATTGTGTTCTTCGGAGCACAAGCGCGGTAACGCATTCGAACTTGGTTAG	5	0.125	No Hit
NTTTGCATCTCAGTTAGGGAATATTAACCCTATTCCCTTTCGACAAGCGG	5	0.125	No Hit
NGATAGAATAATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTC	5	0.125	No Hit
NTACTCCGCCGTTGCGACATGTGCGTTGTCTAGCGCCAGGTCGTAACGAG	5	0.125	No Hit
NTCTTTGGCTGCTTCGAAATGTGGCGTATAGGTGTAAGTTTCCAGCAGTG	5	0.125	No Hit
NTCTTCAAGGATCGAACTTGAATATTTGCTACTACCATTACGATCTGCAC	5	0.125	No Hit
NGATAGCTGGATCTCAGGCAGTTATATTCGGTAAAGCTAATGATTAGAGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0125	0.0
30-31	0.0	0.0	0.0	0.025	0.0
32-33	0.0	0.0	0.0	0.05	0.0
34-35	0.0	0.0	0.0	0.0875	0.0
36-37	0.0	0.0	0.0	0.1125	0.0
38-39	0.0	0.0	0.0	0.26249999999999996	0.0
40-41	0.0	0.0	0.0	0.4	0.0
42-43	0.0	0.0	0.0	0.575	0.0
44-45	0.0	0.0	0.0	0.675	0.0
46-47	0.0	0.0	0.0	0.8375	0.0
48-49	0.0	0.0	0.0	1.0	0.0
50-51	0.0	0.0	0.0	1.1625	0.0
52-53	0.0	0.0	0.0	1.3125	0.0
54-55	0.0	0.0	0.0	1.3875	0.0
56-57	0.0	0.0	0.0	1.4874999999999998	0.0
58-59	0.0	0.0	0.0	1.6124999999999998	0.0
60-61	0.0	0.0	0.0	1.75	0.0
62-63	0.0	0.0	0.0	1.9625	0.0
64-65	0.0	0.0	0.0	2.025	0.0
66-67	0.0	0.0	0.0	2.2125	0.0
68-69	0.0	0.0	0.0	2.3875	0.0
70-71	0.0	0.0	0.0	2.6	0.0
72-73	0.0	0.0	0.0	2.7625	0.0
74-75	0.0	0.0	0.0	2.9	0.0
76-77	0.0	0.0	0.0	3.0	0.0
78-79	0.0	0.0	0.0	3.2	0.0
80-81	0.0	0.0	0.0	3.3625	0.0
82-83	0.0	0.0	0.0	3.4625	0.0
84-85	0.0	0.0	0.0	3.5875	0.0
86-87	0.0	0.0	0.0	3.75	0.0
88-89	0.0	0.0	0.0	3.9375	0.0
90-91	0.0	0.0	0.0	4.1625	0.0
92-93	0.0	0.0	0.0	4.4125	0.0
94-95	0.0	0.0	0.0	4.6875	0.0
96-97	0.0	0.0	0.0	4.725	0.0
98-99	0.0	0.0	0.0	4.987500000000001	0.0
100-101	0.0	0.0	0.0	5.2875	0.0
102-103	0.0	0.0	0.0	5.4625	0.0
104-105	0.0	0.0	0.0	5.5875	0.0
106-107	0.0	0.0	0.0	5.875	0.0
108-109	0.0	0.0	0.0	6.15	0.0
110-111	0.0	0.0	0.0	6.3125	0.0
112-113	0.0	0.0	0.0	6.6125	0.0
114-115	0.0	0.0	0.0	6.875	0.0
116-117	0.0	0.0	0.0	7.175000000000001	0.0
118-119	0.0	0.0	0.0	7.4875	0.0
120-121	0.0	0.0	0.0	7.925	0.0
122-123	0.0	0.0	0.0	8.3875	0.0
124-125	0.0	0.0	0.0	8.649999999999999	0.0
126-127	0.0	0.0	0.0	8.95	0.0
128-129	0.0	0.0	0.0	9.2375	0.0
130-131	0.0	0.0	0.0	9.5	0.0
132-133	0.0	0.0	0.0	9.962499999999999	0.0
134-135	0.0	0.0	0.0	10.350000000000001	0.0
136-137	0.0	0.0	0.0	10.55	0.0
138	0.0	0.0	0.0	10.725	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGG	10	0.0071204724	142.99998	2
AGTACAT	30	0.001565453	23.833334	8
AGAGTAC	30	0.001565453	23.833334	6
GAGTACA	30	0.001565453	23.833334	7
ACATGGG	35	0.0038330643	20.42857	3
AAAAAAA	175	0.0011096502	8.171429	135-139
>>END_MODULE
Read 1428272 spots for SRR13919973.sra
Written 1428272 spots for SRR13919973.sra
Read 1428272 spots for SRR13919973.sra
Written 1428272 spots for SRR13919973.sra
Read 1428272 spots for SRR13919973.sra
Written 1428272 spots for SRR13919973.sra
Read 1428272 spots for SRR13919973.sra
Written 1428272 spots for SRR13919973.sra
Read 1428272 spots for SRR13919973.sra
Written 1428272 spots for SRR13919973.sra
Read 1428272 spots for SRR13919973.sra
Written 1428272 spots for SRR13919973.sra
Read 1428272 spots for SRR13919973.sra
Written 1428272 spots for SRR13919973.sra
Read 1428272 spots for SRR13919973.sra
Written 1428272 spots for SRR13919973.sra
Read 1428272 spots for SRR13919973.sra
Written 1428272 spots for SRR13919973.sra
Read 1428272 spots for SRR13919973.sra
Written 1428272 spots for SRR13919973.sra
Read 1428272 spots for SRR13919973.sra
Written 1428272 spots for SRR13919973.sra
Read 1428272 spots for SRR13919973.sra
Written 1428272 spots for SRR13919973.sra
Read 1428272 spots for SRR13919973.sra
Written 1428272 spots for SRR13919973.sra
Read 1428272 spots for SRR13919973.sra
Written 1428272 spots for SRR13919973.sra
Read 1428272 spots for SRR13919973.sra
Written 1428272 spots for SRR13919973.sra
Read 1428272 spots for SRR13919973.sra
Written 1428272 spots for SRR13919973.sra
SRR ids: ['SRR13919973.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_jjv8kmaf
SRR13919973.sra spots: 22852352
blocks: [[1, 1428272], [1428273, 2856544], [2856545, 4284816], [4284817, 5713088], [5713089, 7141360], [7141361, 8569632], [8569633, 9997904], [9997905, 11426176], [11426177, 12854448], [12854449, 14282720], [14282721, 15710992], [15710993, 17139264], [17139265, 18567536], [18567537, 19995808], [19995809, 21424080], [21424081, 22852352]]
SRR13919973 file size 8392684
SRR13919973 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 16 -o SRR13919973 SRR13919973_1.fastq SRR13919973_2.fastq
Input file:	SRR13919973_1.fastq
Paired file:	SRR13919973_2.fastq
trimmed:	SRR13919973-trimmed-pair1.fastq, SRR13919973-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	16
Thu Dec  2 02:07:26 2021 >> started

Thu Dec  2 02:10:02 2021 >> done (155.802s)
22852352 read pairs processed; of these:
   35487 ( 0.16%) short read pairs filtered out after trimming by size control
   11312 ( 0.05%) empty read pairs filtered out after trimming by size control
22805553 (99.80%) read pairs available; of these:
 1052380 ( 4.61%) trimmed read pairs available after processing
21753173 (95.39%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 81	       1	  0.00%
 82	       7	  0.00%
 83	      90	  0.00%
 84	    2884	  0.01%
 85	    3923	  0.02%
 86	    2840	  0.01%
 87	    5110	  0.02%
 88	    4604	  0.02%
 89	    1680	  0.01%
 90	    3229	  0.01%
 91	    3117	  0.01%
 92	    2811	  0.01%
 93	    4117	  0.02%
 94	    1362	  0.01%
 95	    2940	  0.01%
 96	    1613	  0.01%
 97	    3005	  0.01%
 98	    1787	  0.01%
 99	    2737	  0.01%
100	    2064	  0.01%
101	    1360	  0.01%
102	     117	  0.00%
103	     738	  0.00%
104	    1043	  0.00%
105	    1004	  0.00%
106	     488	  0.00%
107	     715	  0.00%
108	       8	  0.00%
109	     618	  0.00%
110	      68	  0.00%
111	    1009	  0.00%
112	     204	  0.00%
113	       0	  0.00%
114	       0	  0.00%
115	      22	  0.00%
116	       0	  0.00%
117	       9	  0.00%
118	       9	  0.00%
119	    1067	  0.00%
120	      55	  0.00%
121	      66	  0.00%
122	      67	  0.00%
123	       0	  0.00%
124	     322	  0.00%
125	       7	  0.00%
126	     112	  0.00%
127	      16	  0.00%
128	      31	  0.00%
129	     538	  0.00%
130	      63	  0.00%
131	     153	  0.00%
132	      33	  0.00%
133	     917	  0.00%
134	      51	  0.00%
135	    2379	  0.01%
136	    1753	  0.01%
137	    1942	  0.01%
138	     993	  0.00%
139	      19	  0.00%
140	    1051	  0.00%
141	     126	  0.00%
142	     796	  0.00%
143	     204	  0.00%
144	     514	  0.00%
145	    1381	  0.01%
146	    5868	  0.03%
147	   16327	  0.07%
148	   68764	  0.30%
149	  889432	  3.90%
150	21753173	 95.39%
22805553 reads passed initial QC


criterion=sequence-density
sequence-density=9.81
sequence-density-rank=1
fanout-score=45.74
fanout-score-rank=1
prefix-density=10.34
prefix-fanout=43.4
sequence=CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA


criterion=fanout-score
sequence-density=9.81
sequence-density-rank=1
fanout-score=45.74
fanout-score-rank=1
prefix-density=10.34
prefix-fanout=43.4
sequence=CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA


criterion=sequence-density
sequence-density=9.93
sequence-density-rank=1
fanout-score=45.79
fanout-score-rank=1
prefix-density=10.45
prefix-fanout=43.5
sequence=CTGTCTCTTATACACATCTGACGCTGCCGACGAAGCTAGAAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAA


criterion=fanout-score
sequence-density=9.93
sequence-density-rank=1
fanout-score=45.79
fanout-score-rank=1
prefix-density=10.45
prefix-fanout=43.5
sequence=CTGTCTCTTATACACATCTGACGCTGCCGACGAAGCTAGAAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 818: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 819: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 16 -x CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA -y CTGTCTCTTATACACATCTGACGCTGCCGACGAAGCTAGAAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAA -o SRR13919973 SRR13919973_1.fastq SRR13919973_2.fastq
Input file:	SRR13919973_1.fastq
Paired file:	SRR13919973_2.fastq
trimmed:	SRR13919973-trimmed-pair1.fastq, SRR13919973-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCT
-- paired 3' end adapter sequence (-y):	CTGTCTCTTATACACATCTGACGCTGCCGACGAAGCTAGAAGTGTAGATCTCGGTGGTCGCCGT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	16
Thu Dec  2 02:21:01 2021 >> started

Thu Dec  2 02:23:40 2021 >> done (159.007s)
18244443 read pairs processed; of these:
      50 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
18244393 (100.00%) read pairs available; of these:
 2183538 (11.97%) trimmed read pairs available after processing
16060855 (88.03%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       7	  0.00%
 19	      17	  0.00%
 20	      20	  0.00%
 21	      13	  0.00%
 22	      29	  0.00%
 23	      34	  0.00%
 24	      32	  0.00%
 25	      38	  0.00%
 26	      77	  0.00%
 27	     335	  0.00%
 28	     646	  0.00%
 29	    1299	  0.01%
 30	    2933	  0.02%
 31	    3681	  0.02%
 32	    2950	  0.02%
 33	    3245	  0.02%
 34	    3687	  0.02%
 35	    4172	  0.02%
 36	    7297	  0.04%
 37	    8663	  0.05%
 38	    9322	  0.05%
 39	   10943	  0.06%
 40	    8828	  0.05%
 41	    9496	  0.05%
 42	    8543	  0.05%
 43	    9235	  0.05%
 44	    9513	  0.05%
 45	    9760	  0.05%
 46	   10440	  0.06%
 47	   10410	  0.06%
 48	   10510	  0.06%
 49	   11011	  0.06%
 50	   10716	  0.06%
 51	   10924	  0.06%
 52	    9469	  0.05%
 53	   11052	  0.06%
 54	   10481	  0.06%
 55	   11846	  0.06%
 56	   11916	  0.07%
 57	   10564	  0.06%
 58	   12483	  0.07%
 59	   11852	  0.06%
 60	   12721	  0.07%
 61	   12711	  0.07%
 62	   11377	  0.06%
 63	   12666	  0.07%
 64	   12173	  0.07%
 65	   12085	  0.07%
 66	   13276	  0.07%
 67	   12308	  0.07%
 68	   14137	  0.08%
 69	   12104	  0.07%
 70	   13746	  0.08%
 71	   12392	  0.07%
 72	   13406	  0.07%
 73	   12914	  0.07%
 74	   13298	  0.07%
 75	   12552	  0.07%
 76	   13869	  0.08%
 77	   14373	  0.08%
 78	   17694	  0.10%
 79	   13444	  0.07%
 80	   17373	  0.10%
 81	   14352	  0.08%
 82	   16103	  0.09%
 83	   15992	  0.09%
 84	   15937	  0.09%
 85	   18469	  0.10%
 86	   17294	  0.09%
 87	   19171	  0.11%
 88	   19503	  0.11%
 89	   17736	  0.10%
 90	   21611	  0.12%
 91	   21031	  0.12%
 92	   20342	  0.11%
 93	   22011	  0.12%
 94	   17565	  0.10%
 95	   19646	  0.11%
 96	   18661	  0.10%
 97	   20549	  0.11%
 98	   18771	  0.10%
 99	   22483	  0.12%
100	   20033	  0.11%
101	   20696	  0.11%
102	   19825	  0.11%
103	   19289	  0.11%
104	   20963	  0.11%
105	   21592	  0.12%
106	   18543	  0.10%
107	   22017	  0.12%
108	   18665	  0.10%
109	   23000	  0.13%
110	   22977	  0.13%
111	   23201	  0.13%
112	   23935	  0.13%
113	   20918	  0.11%
114	   23557	  0.13%
115	   23054	  0.13%
116	   24295	  0.13%
117	   23506	  0.13%
118	   22776	  0.12%
119	   25484	  0.14%
120	   25715	  0.14%
121	   28572	  0.16%
122	   26373	  0.14%
123	   25076	  0.14%
124	   25592	  0.14%
125	   22931	  0.13%
126	   26349	  0.14%
127	   25048	  0.14%
128	   27923	  0.15%
129	   30082	  0.16%
130	   28816	  0.16%
131	   29841	  0.16%
132	   25418	  0.14%
133	   27491	  0.15%
134	   28673	  0.16%
135	   31616	  0.17%
136	   31037	  0.17%
137	   32773	  0.18%
138	   29755	  0.16%
139	   28987	  0.16%
140	   33227	  0.18%
141	   31018	  0.17%
142	   33251	  0.18%
143	   30603	  0.17%
144	   30852	  0.17%
145	   37455	  0.21%
146	   38484	  0.21%
147	   47445	  0.26%
148	   84309	  0.46%
149	  678159	  3.72%
150	15296861	 83.84%


criterion=sequence-density
sequence-density=1.37
sequence-density-rank=1
fanout-score=2.52
fanout-score-rank=30
prefix-density=3.45
prefix-fanout=1.0
sequence=GAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCAAGGACCATCTGTCACCTCTGTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGCGATCAGGATACTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCAAGCACAATCACTAGTCCGCCATCAACCCGAAAGTATCAAGCAGACAGAACTGCGCCGGACAAGCATCAACCAACTGGCCAACCGTTCCAACCACTGGAGCCAATCCTTTTCCCGAAGTTACGGATCTAGTTTGCCGACTTCCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTAATGCGGATATCGGTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACCGCGGTGCTTTTCGGAAACAACGTCCCCATCTCTGGCTGAACCAATTCCAGGGAGT


criterion=fanout-score
sequence-density=0.11
sequence-density-rank=28
fanout-score=37.34
fanout-score-rank=1
prefix-density=3.75
prefix-fanout=1.1
sequence=AAAGCTTGCTTGATCTTGACTTTCAGTACGAGTACAGACCGCGAAAGCGTGGCCTATCGATCCTTTTAATCCTGATTGTTTCAGGTAAGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTCCATAGCGACGTTGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGCGAAGCAGAATTCGCCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGTTGACTTGTTATTGCGAAAGTAATCCTGCTTAGTACGAGAGGAACAGCGGGTTCAAACATTTGGTTCATAGACTTGATCGACAGATCAATGGTCTGAAGCTACCATTTGAGAGATTATAACTGAACGCCTCTAAGTTAGAATCTCGCCTTGTCAAGGCGAAAATTTCTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGGCAACACGAGAGCTTATGCC


criterion=sequence-density
sequence-density=1.34
sequence-density-rank=1
fanout-score=2.57
fanout-score-rank=31
prefix-density=3.45
prefix-fanout=1.0
sequence=GAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCAAGGACCATCTGTCACCTCTGTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGCGATCAGGATACTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCAAGCACAATCACTAGTCCGCCATCAACCCGAAAGTATCAAGCAGACAGAACTGCGCCGGACAAGCATCAACCAACTGGCCAACCGTTCCAACCACTGGAGCCAATCCTTTTCCCGAAGTTACGGATCTAGTTTGCCGACTTCCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTAATGCGGATATCGGTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACCGCGGTGCTTTTCGGAAACAACGTCCCCATCTCTGGCTGAACCAATTCCAGGGAGT


criterion=fanout-score
sequence-density=0.11
sequence-density-rank=27
fanout-score=35.99
fanout-score-rank=1
prefix-density=3.72
prefix-fanout=1.1
sequence=AAAGCTTGCTTGATCTTGACTTTCAGTACGAGTACAGACCGCGAAAGCGTGGCCTATCGATCCTTTTAATCCTGATTGTTTCAGGTAAGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTCCATAGCGACGTTGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGCGAAGCAGAATTCGCCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGTTGACTTGTTATTGCGAAAGTAATCCTGCTTAGTACGAGAGGAACAGCGGGTTCAAACATTTGGTTCATAGACTTGATCGACAGATCAATGGTCTGAAGCTACCATTTGAGAGATTATAACTGAACGCCTCTAAGTTAGAATCTCGCCTTGTCAAGGCGAAAATTTCTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGGCAACACGAGAGCTTATGCC
SRR13919973 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Dec 02 02:28:24
                             Started mapping on |	Dec 02 02:28:24
                                    Finished on |	Dec 02 02:46:26
       Mapping speed, Million of reads per hour |	75.88

                          Number of input reads |	22805503
                      Average input read length |	290
                                    UNIQUE READS:
                   Uniquely mapped reads number |	19982703
                        Uniquely mapped reads % |	87.62%
                          Average mapped length |	287.84
                       Number of splices: Total |	3161988
            Number of splices: Annotated (sjdb) |	3025505
                       Number of splices: GT/AG |	3121656
                       Number of splices: GC/AG |	15864
                       Number of splices: AT/AC |	827
               Number of splices: Non-canonical |	23641
                      Mismatch rate per base, % |	0.28%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.41
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.21
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1773327
             % of reads mapped to multiple loci |	7.78%
        Number of reads mapped to too many loci |	36320
             % of reads mapped to too many loci |	0.16%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.30%
                     % of reads unmapped: other |	0.14%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1051324	1051324	1051324
N_multimapping	1773327	1773327	1773327
N_noFeature	592008	10298395	10102484
N_ambiguous	218248	28757	28923
UnstrandedReadsAssigned:19172447 PositiveStrandReadsAssigned:9655551 NegativeStrandReadsAssigned:9851296
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR13919973 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 33,746
[index] number of k-mers: 28,927,460
[index] number of equivalence classes: 54,013
[quant] running in paired-end mode
[quant] will process pair 1: SRR13919973-trimmed-pair1.fastq
                             SRR13919973-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 22,805,503 reads, 4,109,953 reads pseudoaligned
[quant] estimated average fragment length: 278.669
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,079 rounds

  33747 SRR13919973.ke.tsv
  46739 SRR13919973.se.tsv
  80486 total
==> SRR13919973.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Y110A7A.10	1787	1509.33	464	59.8344
F27C8.1	1940	1662.33	115	13.4647
F07C3.7	1728	1450.33	142	19.0563
F52H2.2a	1739	1461.33	116.657	15.5374
F52H2.2b	1840	1562.33	77.3431	9.63532
T13A10.10a	1734	1456.33	0	0
T13A10.10b	1598	1320.33	2	0.294825
C55C2.5a	1840	1562.33	131.334	16.3614
C55C2.5b	1606	1328.33	13.3445	1.95531

==> SRR13919973.se.tsv <==
WBGene00197333	0
WBGene00198386	0
WBGene00015153	0
WBGene00002061	82
WBGene00255704	30
WBGene00235314	87
WBGene00001177	1735
WBGene00169236	0
WBGene00219784	0
WBGene00015152	1
SRR13919973 completed mapping pipeline successfully
