Starting /dee2/code/volunteer_pipeline.sh SRR13919969
    current disk space = 702171680
    free memory = 25630208 
SRR13919969 SRAfilesize
dc568fc9abed7e447128604bc751da22  SRR13919969.sra
SRR13919969.sra file validated
SRR13919969 is paired end
SRR13919969 is conventional basespace
SRR13919969 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13919969_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40875	32.0	32.0	32.0	32.0	32.0
2	31.52125	32.0	32.0	32.0	32.0	32.0
3	35.42	37.0	37.0	37.0	32.0	37.0
4	36.10375	37.0	37.0	37.0	32.0	37.0
5	36.38625	37.0	37.0	37.0	37.0	37.0
6	39.9125	41.0	41.0	41.0	37.0	41.0
7	39.9925	41.0	41.0	41.0	37.0	41.0
8	40.00575	41.0	41.0	41.0	37.0	41.0
9	39.99	41.0	41.0	41.0	37.0	41.0
10-14	40.033950000000004	41.0	41.0	41.0	37.0	41.0
15-19	40.07115	41.0	41.0	41.0	37.0	41.0
20-24	39.9701	41.0	41.0	41.0	37.0	41.0
25-29	39.933800000000005	41.0	41.0	41.0	37.0	41.0
30-34	39.84015	41.0	41.0	41.0	37.0	41.0
35-39	39.67825	41.0	41.0	41.0	37.0	41.0
40-44	39.80985	41.0	41.0	41.0	37.0	41.0
45-49	39.66815	41.0	41.0	41.0	37.0	41.0
50-54	39.56235	41.0	41.0	41.0	37.0	41.0
55-59	39.4053	41.0	41.0	41.0	37.0	41.0
60-64	39.39115	41.0	41.0	41.0	37.0	41.0
65-69	39.3221	41.0	41.0	41.0	37.0	41.0
70-74	39.3335	41.0	41.0	41.0	37.0	41.0
75-79	39.09845	41.0	40.2	41.0	35.0	41.0
80-84	39.33475	41.0	41.0	41.0	37.0	41.0
85-89	39.34815	41.0	41.0	41.0	37.0	41.0
90-94	39.363749999999996	41.0	41.0	41.0	37.0	41.0
95-99	39.3224	41.0	41.0	41.0	37.0	41.0
100-104	39.11655	41.0	41.0	41.0	35.0	41.0
105-109	38.9994	41.0	41.0	41.0	33.0	41.0
110-114	39.002250000000004	41.0	41.0	41.0	33.0	41.0
115-119	38.7907	41.0	41.0	41.0	32.0	41.0
120-124	38.60705	41.0	40.2	41.0	32.0	41.0
125-129	38.31425	41.0	37.8	41.0	32.0	41.0
130-134	38.23965	41.0	37.0	41.0	32.0	41.0
135-139	38.02255	41.0	37.0	41.0	32.0	41.0
140-144	37.90195	41.0	37.0	41.0	31.0	41.0
145-149	37.5281	41.0	37.0	41.0	27.0	41.0
150	35.3905	41.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1201	1	0.0
1201	2	0.0
1201	3	0.0
1201	4	0.0
1201	5	0.0
1201	6	0.0
1201	7	0.0
1201	8	0.0
1201	9	0.0
1201	10-14	0.0
1201	15-19	0.0
1201	20-24	0.0
1201	25-29	0.0
1201	30-34	0.0
1201	35-39	0.0
1201	40-44	0.0
1201	45-49	0.0
1201	50-54	0.0
1201	55-59	0.0
1201	60-64	0.0
1201	65-69	0.0
1201	70-74	0.0
1201	75-79	0.0
1201	80-84	0.0
1201	85-89	0.0
1201	90-94	0.0
1201	95-99	0.0
1201	100-104	0.0
1201	105-109	0.0
1201	110-114	0.0
1201	115-119	0.0
1201	120-124	0.0
1201	125-129	0.0
1201	130-134	0.0
1201	135-139	0.0
1201	140-144	0.0
1201	145-149	0.0
1201	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	2.0
23	3.0
24	6.0
25	10.0
26	13.0
27	14.0
28	21.0
29	28.0
30	31.0
31	46.0
32	70.0
33	78.0
34	88.0
35	105.0
36	135.0
37	187.0
38	256.0
39	484.0
40	2422.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.02678120262759	19.984840828701365	12.12733703890854	24.861040929762506
2	16.025	20.95	39.15	23.875
3	17.424999999999997	24.525	29.75	28.299999999999997
4	14.6	15.5	38.5	31.4
5	14.325	36.575	34.525	14.575
6	30.0	37.4	17.75	14.85
7	28.95	32.2	19.7	19.15
8	24.9	34.050000000000004	21.85	19.2
9	29.349999999999998	14.05	19.25	37.35
10-14	25.174999999999997	23.64	26.490000000000002	24.695
15-19	24.59	27.355	24.715	23.34
20-24	24.925	26.174999999999997	24.6	24.3
25-29	24.195	26.91	24.73	24.165
30-34	23.7	26.090000000000003	25.905	24.305
35-39	25.615	26.165	24.02	24.2
40-44	23.705000000000002	25.495	25.82	24.98
45-49	24.305	25.05	25.840000000000003	24.805
50-54	24.7823040736663	26.468821939745773	24.482033830447403	24.266840156140525
55-59	24.41	26.57	25.305	23.715
60-64	24.465688973422093	26.222533660343363	25.44671905500776	23.86505831122679
65-69	24.127412741274128	25.61256125612561	24.722472247224722	25.537553755375537
70-74	24.45	24.245	26.555	24.75
75-79	23.91217365209563	25.527658297489246	26.257877363208966	24.302290687206163
80-84	25.23392544408306	25.899424568426323	24.748561421065798	24.11808856642482
85-89	24.63985594237695	25.165066026410564	25.195078031212486	25.0
90-94	24.099999999999998	25.555	25.235000000000003	25.11
95-99	25.212521252125214	25.467546754675467	24.3974397439744	24.922492249224923
100-104	24.182418241824184	24.902490249024904	24.847484748474848	26.067606760676064
105-109	23.952395239523952	25.17751775177518	25.57255725572557	25.297529752975294
110-114	23.645	25.405	25.145	25.805
115-119	23.005	26.93	24.959999999999997	25.105
120-124	24.274854970994202	26.325265053010604	24.50490098019604	24.89497899579916
125-129	23.884553821528613	26.745698279311725	24.68487394957983	24.68487394957983
130-134	24.48479391756703	25.290116046418564	25.08003201280512	25.145058023209284
135-139	24.314725890356144	26.31552621048419	24.804921968787514	24.56482593037215
140-144	23.679471788715485	26.16046418567427	25.120048019207687	25.04001600640256
145-149	23.574144486692013	27.37142285371223	24.809885931558938	24.244546728036823
150	23.74874874874875	26.001001001001	22.872872872872875	27.37737737737738
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	2.5
9	2.0
10	1.0
11	0.5
12	0.5
13	1.0
14	1.0
15	0.5
16	0.0
17	0.0
18	0.5
19	0.5
20	0.0
21	0.5
22	1.0
23	0.5
24	1.5
25	2.5
26	1.0
27	1.5
28	4.0
29	3.5
30	4.5
31	8.5
32	8.0
33	8.0
34	12.0
35	15.5
36	13.0
37	14.5
38	27.0
39	36.0
40	48.0
41	69.5
42	82.0
43	91.0
44	124.5
45	170.5
46	234.5
47	274.0
48	332.0
49	356.0
50	308.5
51	316.0
52	299.0
53	258.0
54	213.0
55	149.0
56	122.0
57	111.0
58	103.0
59	83.0
60	40.5
61	19.5
62	13.0
63	5.0
64	1.5
65	0.5
66	0.5
67	0.0
68	0.5
69	0.5
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.05
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.09
55-59	0.0
60-64	0.105
65-69	0.01
70-74	0.0
75-79	0.03
80-84	0.075
85-89	0.04
90-94	0.0
95-99	0.01
100-104	0.01
105-109	0.01
110-114	0.0
115-119	0.0
120-124	0.02
125-129	0.04
130-134	0.04
135-139	0.04
140-144	0.04
145-149	0.06
150	0.1
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.82468924132019	42.475
2	11.315902271753107	13.200000000000001
3	6.600942991855979	11.55
4	3.557651093013288	8.3
5	1.5859408486926703	4.625
6	1.3716245177882553	4.8
7	0.9001285897985427	3.675
8	0.47149592798971285	2.1999999999999997
9	0.17145306472353192	0.8999999999999999
>10	1.2001714530647234	8.275
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCA	19	0.475	No Hit
GTCTAGCGCCAGGTCGTAACGAGTAGGAAGGTCGTGGCGGTTGCGTTGAA	18	0.44999999999999996	No Hit
GTATAGGTCTCTCGCTTAAGCGCCATCCATTTTCAGGGCTAGTTGATTCG	17	0.42500000000000004	No Hit
GTAGTTGGATGTGGGTCAGTCGATCCTAAGGTACTGGCGAACGCCTTGTA	16	0.4	No Hit
GTCTTTGAGCGACTAACCAAGTTCGAATGCGTTACCGCGCTTGTGCTCCG	14	0.35000000000000003	No Hit
GTCTTTGGCTGCTTCGAAATGTGGCGTATAGGTGTAAGTTTCCAGCAGTG	14	0.35000000000000003	No Hit
GTTCTGACGTGCAAATCGATCGATAGACTTGGGTATAGGGGCGAAAGACT	12	0.3	No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCGCT	12	0.3	No Hit
GTGTTAAAACCTTGCGGCGAAATGAAAGTAAAGGTCAGTCTCGAATTGGC	12	0.3	No Hit
GCGCTAGACAACGCACATGTCGCAACGGCGGAGTATAGGTCTCTCGCTTA	11	0.27499999999999997	No Hit
TCCAACTACTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAAGGATC	11	0.27499999999999997	No Hit
TATTAACCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGCTTTTCG	11	0.27499999999999997	No Hit
TTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACCGCGGTGCTTT	11	0.27499999999999997	No Hit
GGATACTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCAA	11	0.27499999999999997	No Hit
ATCCTAAGGTACTGGCGAACGCCTTGTATCATCGGTGGCGAAAAGCTTGC	11	0.27499999999999997	No Hit
GACCTATACTCCGCCGTTGCGACATGTGCGTTGTCTAGCGCCAGGTCGTA	11	0.27499999999999997	No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTAATGCGGATATCGGTACGATTGA	10	0.25	No Hit
GCCCTTAAAACACCAAGGGAGGCTATTAATTTGCACTCAATCGTACCGAT	10	0.25	No Hit
GTCCAAGCACACACCAGAAAGCGTCCGCAAGCAAGCACAATCACTAGTCC	10	0.25	No Hit
TTGTTACACACTCCTTAGCGGATACCGACTTCCATGGCCACCGTCCTGCT	10	0.25	No Hit
TATCAACGCAGAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCAA	10	0.25	No Hit
CCCTGGAATTGGTTCAGCCAGAGATGGGGACGTTGTTTCCGAAAAGCACC	10	0.25	No Hit
ATTCCAGGGAGTCCGTTCCTTAACTAGAGAAGAAAACTCTAGCTCGGTCT	10	0.25	No Hit
GTTTAGATGAGCGATCAGGATACTAGAACCGAGGTCCAAGCACACACCAG	10	0.25	No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAAT	10	0.25	No Hit
ACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTAATGCGGAT	10	0.25	No Hit
CTCAAAGACCGAGCTAGAGTTTTCTTCTCTAGTTAAGGAACGGACTCCCT	10	0.25	No Hit
CTCCTTAGCGGATACCGACTTCCATGGCCACCGTCCTGCTGTCAATATCA	10	0.25	No Hit
ACTTGTGACTGTGCAGAGGTTGAGCAGTTGGCAAACGACCCGAAAGATGG	9	0.22499999999999998	No Hit
GTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGG	9	0.22499999999999998	No Hit
GTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCAAGGACCATCTGTC	9	0.22499999999999998	No Hit
GGGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGGAGCACA	9	0.22499999999999998	No Hit
GTATCATCGGTGGCGAAAAGCTTGCTTTTAGTCCCCGCTTGTCGAAAGGG	8	0.2	No Hit
GTGCAGATCGTAATGGTAGTAGCAAATATTCAAGTTCGATCCTTGAAGAC	8	0.2	No Hit
ATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTAATGCGGATATCGG	8	0.2	No Hit
ATCAGTGACAGTCAACGTCCGCAAGGACCATCTGTCACCTCTGTTTTAAT	8	0.2	No Hit
CCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACC	8	0.2	No Hit
ATCGTACCGATATCCGCATTAGGTCTCCAAGGTGAACAGCCTCTAGTCGA	8	0.2	No Hit
CCAGAAAGCGTCCGCAAGCAAGCACAATCACTAGTCCGCCATCAACCCGA	8	0.2	No Hit
CCTTAGGATCGACTGACCCACATCCAACTACTGTTCACGTGGAACCCTTC	8	0.2	No Hit
GTCCTCAACCTATTCTCAAACTTTCAATGGATATGAAGTTGCAGTTTCTT	8	0.2	No Hit
CACTAGCGTAACTAAACGCTAGCCGCCACGAACCCACTACCGCCAGCAAG	8	0.2	No Hit
GTTCTGGATAGCGGCACTGTTGGTTCGCTTGCTCCTTGGAGTCGGGTTGC	8	0.2	No Hit
CACCAGAAAGCGTCCGCAAGCAAGCACAATCACTAGTCCGCCATCAACCC	7	0.17500000000000002	No Hit
GGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATG	7	0.17500000000000002	No Hit
GTTATATTCGGTAAAGCTAATGATTAGAGGCCTTGGGGACGTAATGTCCT	7	0.17500000000000002	No Hit
GAATAATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGGGAA	7	0.17500000000000002	No Hit
GTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGC	7	0.17500000000000002	No Hit
CAACTACTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAAGGATCGA	7	0.17500000000000002	No Hit
TCTCGCAAGAGAAGGTGTCTTGCTGGCGGTAGTGGGTTCGTGGCGGCTAG	7	0.17500000000000002	No Hit
TCTCTGGCTGAACCAATTCCAGGGAGTCCGTTCCTTAACTAGAGAAGAAA	7	0.17500000000000002	No Hit
AGGGAATATTAACCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGC	7	0.17500000000000002	No Hit
TAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGT	7	0.17500000000000002	No Hit
GAACTGCGCCGGACAAGCATCAACCAACTGGCCAACCGTTCCAACCACTG	7	0.17500000000000002	No Hit
GGAATAGGGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGG	7	0.17500000000000002	No Hit
CCCTAACTGAGATGCAAAGATTGTGTTCTTCGGAGCACAAGCGCGGTAAC	7	0.17500000000000002	No Hit
GTGTTGCCCATCTCGCAAGAGAAGGTGTCTTGCTGGCGGTAGTGGGTTCG	7	0.17500000000000002	No Hit
CCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAA	7	0.17500000000000002	No Hit
GAATATAACTGCCTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAA	7	0.17500000000000002	No Hit
CCTATACTCCGCCGTTGCGACATGTGCGTTGTCTAGCGCCAGGTCGTAAC	7	0.17500000000000002	No Hit
AACTAGATCCGTAACTTCGGGAAAAGGATTGGCTCCAGTGGTTGGAACGG	7	0.17500000000000002	No Hit
GCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAACTAGATC	7	0.17500000000000002	No Hit
CCTTTTATGGGGTCTCATGAGCGAGAAGTTAGGCACCTTAACTCCACGTT	7	0.17500000000000002	No Hit
GTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACC	7	0.17500000000000002	No Hit
GGCTATTAATTTGCACTCAATCGTACCGATATCCGCATTAGGTCTCCAAG	6	0.15	No Hit
CACGTTGACAGTTCACTAAAGAAACTGCAACTTCATATCCATTGAAAGTT	6	0.15	No Hit
GAATAGGGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGGA	6	0.15	No Hit
TTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGAGACAC	6	0.15	No Hit
GTTTTAACACCCTTTGACTCGCAGTAGACAAGCACTCCGCAATCCGTGTT	6	0.15	No Hit
GTCTTCAAGGATCGAACTTGAATATTTGCTACTACCATTACGATCTGCAC	6	0.15	No Hit
GAACTGGCGCTGTGGGATGAACCAAACGTGGAGTTAAGGTGCCTAACTTC	6	0.15	No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGC	6	0.15	No Hit
GTTACACACTCCTTAGCGGATACCGACTTCCATGGCCACCGTCCTGCTGT	6	0.15	No Hit
GGATGAAGCCAGAGGAAACTCTGGTGGAAGTCCGTATCGGTTCTGACGTG	6	0.15	No Hit
GCCTAAAGTGGGTGATAAACTTCATCTAAGGCTAAATATCGACTCGATTG	6	0.15	No Hit
GATATTGACAGCAGGACGGTGGCCATGGAAGTCGGTATCCGCTAAGGAGT	6	0.15	No Hit
TCGTAATGGTAGTAGCAAATATTCAAGTTCGATCCTTGAAGACTGAAGTG	6	0.15	No Hit
GTTCACCATCTTTCGGGTCGTTTGCCAACTGCTCAACCTCTGCACAGTCA	6	0.15	No Hit
CTTAAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTT	6	0.15	No Hit
CACTTGGACCTCGCATTCACGTTGACAGTTCACTAAAGAAACTGCAACTT	6	0.15	No Hit
AAACCAGAGAGGGTGCGAGCCCCGTTCTGGATAGCGGCACTGTTGGTTCG	6	0.15	No Hit
GTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTT	6	0.15	No Hit
GTCAACGTGAATGCGAGGTCCAAGTGGGCCATTTTTGGTAAGCAGAACTG	6	0.15	No Hit
CCCAAGGCCTCTAATCATTAGCTTTACCGAATATAACTGCCTGAGATCCA	6	0.15	No Hit
TCTGTCACCTCTGTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCG	6	0.15	No Hit
CCTTAGCGGATACCGACTTCCATGGCCACCGTCCTGCTGTCAATATCAAC	6	0.15	No Hit
ACGTCACACACACTAGCGTAACTAAACGCTAGCCGCCACGAACCCACTAC	6	0.15	No Hit
ATCCTGCTCAGGCATAGTTCACCATCTTTCGGGTCGTTTGCCAACTGCTC	6	0.15	No Hit
AATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGAGAC	6	0.15	No Hit
GTCAATATCAACCAACACCTTTTATGGGGTCTCATGAGCGAGAAGTTAGG	6	0.15	No Hit
GTTCTAGCCCGACAGTGTTGCCCATCTCGCAAGAGAAGGTGTCTTGCTGG	6	0.15	No Hit
GTGTTTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACCGCGGTG	6	0.15	No Hit
GTGCTTGTCTACTGCGAGTCAAAGGGTGTTAAAACCTTGCGGCGAAATGA	6	0.15	No Hit
CTCCTAGTGTATCTCGTTATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC	6	0.15	No Hit
TACTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCAAGCA	6	0.15	No Hit
CCATTACGATCTGCACTGACGGAAGCTCCAGCCGAGCCTACGCTCATAGC	6	0.15	No Hit
ATATGAAGTTGCAGTTTCTTTAGTGAACTGTCAACGTGAATGCGAGGTCC	5	0.125	No Hit
CCTTAACTCCACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAA	5	0.125	No Hit
CTTCATATCCATTGAAAGTTTGAGAATAGGTTGAGGACATTACGTCCCCA	5	0.125	No Hit
ATCTAGTAGCTGGTTCCTTCCGAAGTTTCCCTCAGGATAGCTGGATCTCA	5	0.125	No Hit
TTCCTTAACTAGAGAAGAAAACTCTAGCTCGGTCTTTGAGCGACTAACCA	5	0.125	No Hit
TTCCTATGCTACGCCGACGGCGTTGGCTGCTCGTTCTAGCCCGACAGTGT	5	0.125	No Hit
TACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTAATG	5	0.125	No Hit
GTTCATCCCACAGCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGACC	5	0.125	No Hit
GTTCCAGTACGGTTGTTTAGATGAGCGATCAGGATACTAGAACCGAGGTC	5	0.125	No Hit
ATATTAACCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGCTTTTC	5	0.125	No Hit
TCCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACC	5	0.125	No Hit
AACAAGTACCGTGAGGGAAAGTTGCAAAGGACTTTGAAGAGAGAGTTCAA	5	0.125	No Hit
CTGGCCAACCGTTCCAACCACTGGAGCCAATCCTTTTCCCGAAGTTACGG	5	0.125	No Hit
AGACAGAACTGCGCCGGACAAGCATCAACCAACTGGCCAACCGTTCCAAC	5	0.125	No Hit
CTTTCAATGGATATGAAGTTGCAGTTTCTTTAGTGAACTGTCAACGTGAA	5	0.125	No Hit
GAACTTAAGCATATCATTTAGCGGAGGAAAAGAAACTAAAAAGGATTCCC	5	0.125	No Hit
GTCTTGAAACACGGATTGCGGAGTGCTTGTCTACTGCGAGTCAAAGGGTG	5	0.125	No Hit
CATTACGTCCCCAAGGCCTCTAATCATTAGCTTTACCGAATATAACTGCC	5	0.125	No Hit
GTACATGGGTCTCAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAG	5	0.125	No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCTATCGAT	5	0.125	No Hit
GGCTATGAGCGTAGGCTCGGCTGGAGCTTCCGTCAGTGCAGATCGTAATG	5	0.125	No Hit
AGCTAATGATTAGAGGCCTTGGGGACGTAATGTCCTCAACCTATTCTCAA	5	0.125	No Hit
CGCCAGTACCTTAGGATCGACTGACCCACATCCAACTACTGTTCACGTGG	5	0.125	No Hit
AGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTAGATGG	5	0.125	No Hit
GTTTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACCGCGGTGCT	5	0.125	No Hit
ATCCCACAGCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGACCTCGC	5	0.125	No Hit
ACCCGAAAGATGGTGAACTATGCCTGAGCAGGATGAAGCCAGAGGAAACT	5	0.125	No Hit
ATGCTACGCCGACGGCGTTGGCTGCTCGTTCTAGCCCGACAGTGTTGCCC	5	0.125	No Hit
CCACAGAAACCGCGGTGCTTTTCGGAAACAACGTCCCCATCTCTGGCTGA	5	0.125	No Hit
CACAATCACTAGTCCGCCATCAACCCGAAAGTATCAAGCAGACAGAACTG	5	0.125	No Hit
ATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTCAAAG	5	0.125	No Hit
GTTGTTTAGATGAGCGATCAGGATACTAGAACCGAGGTCCAAGCACACAC	5	0.125	No Hit
GCCTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTA	5	0.125	No Hit
GTGTTCTTCGGAGTGCAGCGCACCACGGCCCTGTGCGTGTCACTTGTGAC	5	0.125	No Hit
CCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGCGATCAGGA	5	0.125	No Hit
GGATATGAAGTTGCAGTTTCTTTAGTGAACTGTCAACGTGAATGCGAGGT	5	0.125	No Hit
ACGCTAGTGTGTGTGACGTCGGTGTGAAAGTCGACGACGTTTCCGACCCG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0125	0.0
32-33	0.0	0.0	0.0	0.11249999999999999	0.0
34-35	0.0	0.0	0.0	0.2375	0.0
36-37	0.0	0.0	0.0	0.275	0.0
38-39	0.0	0.0	0.0	0.45	0.0
40-41	0.0	0.0	0.0	0.6499999999999999	0.0
42-43	0.0	0.0	0.0	0.7625	0.0
44-45	0.0	0.0	0.0	0.85	0.0
46-47	0.0	0.0	0.0	1.0125	0.0
48-49	0.0	0.0	0.0	1.1625	0.0
50-51	0.0	0.0	0.0	1.3	0.0
52-53	0.0	0.0	0.0	1.425	0.0
54-55	0.0	0.0	0.0	1.5750000000000002	0.0
56-57	0.0	0.0	0.0	1.6875	0.0
58-59	0.0	0.0	0.0	1.825	0.0
60-61	0.0	0.0	0.0	2.0	0.0
62-63	0.0	0.0	0.0	2.15	0.0
64-65	0.0	0.0	0.0	2.2875	0.0
66-67	0.0	0.0	0.0	2.375	0.0
68-69	0.0	0.0	0.0	2.5	0.0
70-71	0.0	0.0	0.0	2.675	0.0
72-73	0.0	0.0	0.0	2.8375	0.0
74-75	0.0	0.0	0.0	3.075	0.0
76-77	0.0	0.0	0.0	3.2249999999999996	0.0
78-79	0.0	0.0	0.0	3.3625	0.0
80-81	0.0	0.0	0.0	3.5	0.0
82-83	0.0	0.0	0.0	3.7125	0.0
84-85	0.0	0.0	0.0	3.8499999999999996	0.0
86-87	0.0	0.0	0.0	3.9875000000000003	0.0
88-89	0.0	0.0	0.0	4.1375	0.0
90-91	0.0	0.0	0.0	4.3625	0.0
92-93	0.0	0.0	0.0	4.6375	0.0
94-95	0.0	0.0	0.0	4.8625	0.0
96-97	0.0	0.0	0.0	5.0875	0.0
98-99	0.0	0.0	0.0	5.35	0.0
100-101	0.0	0.0	0.0	5.6625	0.0
102-103	0.0	0.0	0.0	5.762499999999999	0.0
104-105	0.0	0.0	0.0	5.9875	0.0
106-107	0.0	0.0	0.0	6.2375	0.0
108-109	0.0	0.0	0.0	6.449999999999999	0.0
110-111	0.0	0.0	0.0	6.7	0.0
112-113	0.0	0.0	0.0	6.8625	0.0
114-115	0.0	0.0	0.0	7.1	0.0
116-117	0.0	0.0	0.0	7.4	0.0
118-119	0.0	0.0	0.0	7.6375	0.0
120-121	0.0	0.0	0.0	7.8375	0.0
122-123	0.0	0.0	0.0	7.9750000000000005	0.0
124-125	0.0	0.0	0.0	8.1875	0.0
126-127	0.0	0.0	0.0	8.3875	0.0
128-129	0.0	0.0	0.0	8.6625	0.0
130-131	0.0	0.0	0.0	8.9125	0.0
132-133	0.0	0.0	0.0	9.125	0.0
134-135	0.0	0.0	0.0	9.350000000000001	0.0
136-137	0.0	0.0	0.0	9.712499999999999	0.0
138	0.0	0.0	0.0	9.95	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAAAA	105	9.154928E-5	12.318214	130-134
>>END_MODULE
SRR13919969 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13919969_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	2.0	2.0	2.0	2.0	2.0	2.0
2	31.24625	32.0	32.0	32.0	32.0	32.0
3	31.2	32.0	32.0	32.0	32.0	32.0
4	35.21875	37.0	37.0	37.0	32.0	37.0
5	35.68625	37.0	37.0	37.0	32.0	37.0
6	39.21975	41.0	41.0	41.0	37.0	41.0
7	39.46075	41.0	41.0	41.0	37.0	41.0
8	39.65175	41.0	41.0	41.0	37.0	41.0
9	39.68825	41.0	41.0	41.0	37.0	41.0
10-14	39.509100000000004	41.0	41.0	41.0	37.0	41.0
15-19	39.5655	41.0	41.0	41.0	37.0	41.0
20-24	39.519099999999995	41.0	41.0	41.0	37.0	41.0
25-29	39.491200000000006	41.0	41.0	41.0	37.0	41.0
30-34	39.4402	41.0	41.0	41.0	37.0	41.0
35-39	39.4528	41.0	41.0	41.0	37.0	41.0
40-44	39.43155	41.0	41.0	41.0	37.0	41.0
45-49	39.2937	41.0	41.0	41.0	37.0	41.0
50-54	39.231700000000004	41.0	41.0	41.0	37.0	41.0
55-59	39.301750000000006	41.0	41.0	41.0	37.0	41.0
60-64	39.12935	41.0	41.0	41.0	37.0	41.0
65-69	39.140049999999995	41.0	41.0	41.0	36.0	41.0
70-74	38.870999999999995	41.0	41.0	41.0	35.0	41.0
75-79	38.44035	41.0	40.2	41.0	32.0	41.0
80-84	39.155899999999995	41.0	41.0	41.0	37.0	41.0
85-89	39.188100000000006	41.0	41.0	41.0	37.0	41.0
90-94	39.08535	41.0	41.0	41.0	37.0	41.0
95-99	38.962	41.0	41.0	41.0	35.0	41.0
100-104	38.854200000000006	41.0	41.0	41.0	36.0	41.0
105-109	38.77445	41.0	41.0	41.0	33.0	41.0
110-114	38.635450000000006	41.0	41.0	41.0	33.0	41.0
115-119	38.52125	41.0	41.0	41.0	32.0	41.0
120-124	38.1196	41.0	39.4	41.0	32.0	41.0
125-129	37.814	41.0	38.6	41.0	29.0	41.0
130-134	37.5347	41.0	37.8	41.0	29.0	41.0
135-139	37.343999999999994	41.0	37.0	41.0	27.0	41.0
140-144	37.128699999999995	41.0	37.0	41.0	27.0	41.0
145-149	36.76925000000001	41.0	37.0	41.0	24.0	41.0
150	31.162	37.0	27.0	41.0	12.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1201	1	0.0
1201	2	0.0
1201	3	0.0
1201	4	0.0
1201	5	0.0
1201	6	0.0
1201	7	0.0
1201	8	0.0
1201	9	0.0
1201	10-14	0.0
1201	15-19	0.0
1201	20-24	0.0
1201	25-29	0.0
1201	30-34	0.0
1201	35-39	0.0
1201	40-44	0.0
1201	45-49	0.0
1201	50-54	0.0
1201	55-59	0.0
1201	60-64	0.0
1201	65-69	0.0
1201	70-74	0.0
1201	75-79	0.0
1201	80-84	0.0
1201	85-89	0.0
1201	90-94	0.0
1201	95-99	0.0
1201	100-104	0.0
1201	105-109	0.0
1201	110-114	0.0
1201	115-119	0.0
1201	120-124	0.0
1201	125-129	0.0
1201	130-134	0.0
1201	135-139	0.0
1201	140-144	0.0
1201	145-149	0.0
1201	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	5.0
15	0.0
16	6.0
17	8.0
18	11.0
19	17.0
20	12.0
21	17.0
22	7.0
23	15.0
24	14.0
25	17.0
26	23.0
27	27.0
28	19.0
29	29.0
30	31.0
31	35.0
32	41.0
33	56.0
34	84.0
35	110.0
36	136.0
37	173.0
38	247.0
39	601.0
40	2253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	NaN	NaN	NaN	NaN
2	15.65	20.7	37.724999999999994	25.924999999999997
3	17.4	23.425	31.65	27.525
4	14.499999999999998	15.325	38.824999999999996	31.35
5	13.728432108027006	37.009252313078264	34.358589647411854	14.90372593148287
6	28.95	38.525	17.875	14.649999999999999
7	27.40926157697122	33.76720901126408	21.27659574468085	17.546933667083856
8	27.025	31.474999999999998	20.849999999999998	20.65
9	28.65	15.575	19.45	36.325
10-14	24.461115278819705	23.765941485371343	27.121780445111277	24.651162790697676
15-19	24.759903961584634	26.91076430572229	24.539815926370547	23.789515806322527
20-24	25.386346586646663	26.76169042260565	24.296074018504626	23.55588897224306
25-29	23.731865932966485	27.24862431215608	24.512256128064035	24.50725362681341
30-34	24.295933169926467	25.39642839277675	25.961682757240755	24.345955680056026
35-39	25.041268570856882	26.19178630383673	24.60107048171677	24.165874643589618
40-44	23.880746335851132	26.086739032564655	25.381421639737884	24.65109299184633
45-49	24.72112450602771	25.916662498124154	25.466459906958132	23.89575308889
50-54	24.77486491895137	26.60596357814689	24.61977186311787	23.99939963978387
55-59	24.279567740644385	26.55593356013608	25.290174104462675	23.874324594756853
60-64	24.14448669201521	25.765459275565338	26.115669401640986	23.974384630778466
65-69	24.484690814488694	26.0606363818291	24.36461877126276	25.090054032419452
70-74	24.549639711769416	24.789831865492392	26.36609287429944	24.29443554843875
75-79	23.894115292233785	25.90572457966373	26.2259807846277	23.97417934347478
80-84	25.380304243394715	25.98578863090472	24.574659727782226	24.059247397918334
85-89	24.514611689351483	25.450360288230584	25.65552441953563	24.379503602882306
90-94	24.164164164164166	24.98998998998999	26.141141141141137	24.704704704704707
95-99	25.520520520520524	25.810810810810807	24.284284284284286	24.384384384384383
100-104	24.844875900720574	24.459567654123298	24.649719775820657	26.045836669335472
105-109	23.94894894894895	25.140140140140137	26.036036036036037	24.874874874874877
110-114	24.059247397918334	26.746397117694155	24.234387510008006	24.959967974379506
115-119	23.353353353353352	26.566566566566568	25.285285285285287	24.794794794794793
120-124	24.14431545236189	26.34607686148919	24.894915932746198	24.614691753402724
125-129	24.29443554843875	26.861489191353083	24.59467574059247	24.249399519615693
130-134	24.884907926341075	25.48538831064852	24.734787830264214	24.894915932746198
135-139	24.44088657627458	27.252714264271777	24.37084104668034	23.935558112773304
140-144	23.70659461623136	26.733713599519664	24.822375662964074	24.7373161212849
145-149	23.946973486743374	27.41370685342671	24.39719859929965	24.242121060530263
150	24.730913642052567	27.2090112640801	22.252816020025033	25.807259073842303
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.5
9	1.0
10	0.5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	0.5
17	0.5
18	1.0
19	0.5
20	0.0
21	1.0
22	1.0
23	1.0
24	1.5
25	2.5
26	3.5
27	2.0
28	2.0
29	2.0
30	3.5
31	8.0
32	12.0
33	13.5
34	13.0
35	13.0
36	20.0
37	32.0
38	34.0
39	43.0
40	66.5
41	95.5
42	108.0
43	116.5
44	158.0
45	213.0
46	272.5
47	336.5
48	321.0
49	331.5
50	346.0
51	279.5
52	247.5
53	236.0
54	165.5
55	108.0
56	108.0
57	101.5
58	84.5
59	42.0
60	18.0
61	12.0
62	6.0
63	4.5
64	3.0
65	1.0
66	1.5
67	1.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.5
73	0.5
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	100.0
2	0.0
3	0.0
4	0.0
5	0.025
6	0.0
7	0.125
8	0.0
9	0.0
10-14	0.025
15-19	0.04
20-24	0.025
25-29	0.05
30-34	0.045
35-39	0.045
40-44	0.045
45-49	0.045
50-54	0.06
55-59	0.06
60-64	0.06
65-69	0.06
70-74	0.08
75-79	0.08
80-84	0.08
85-89	0.08
90-94	0.1
95-99	0.1
100-104	0.08
105-109	0.1
110-114	0.08
115-119	0.1
120-124	0.08
125-129	0.08
130-134	0.08
135-139	0.065
140-144	0.06999999999999999
145-149	0.05
150	0.125
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.72500000000001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.60578969774372	43.225
2	10.898254576415496	12.8
3	6.300553426990209	11.1
4	3.4057045551298426	8.0
5	1.9157088122605364	5.625
6	1.1494252873563218	4.05
7	0.9365687526607066	3.85
8	0.4257130693912303	2.0
9	0.29799914857386123	1.575
>10	1.064282673478076	7.775
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NCATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTAATGCGGAT	20	0.5	No Hit
NTCCTAAGGTACTGGCGAACGCCTTGTATCATCGGTGGCGAAAAGCTTGC	15	0.375	No Hit
NTATAGGTCTCTCGCTTAAGCGCCATCCATTTTCAGGGCTAGTTGATTCG	15	0.375	No Hit
NGATACTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCAA	15	0.375	No Hit
NTCTAGCGCCAGGTCGTAACGAGTAGGAAGGTCGTGGCGGTTGCGTTGAA	14	0.35000000000000003	No Hit
NTATCAACGCAGAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCA	14	0.35000000000000003	No Hit
NCTAGAGGCTGTTCACCTTGGAGACCTAATGCGGATATCGGTACGATTGA	14	0.35000000000000003	No Hit
NTCTAGTAGCTGGTTCCTTCCGAAGTTTCCCTCAGGATAGCTGGATCTCA	13	0.325	No Hit
NTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCGCT	12	0.3	No Hit
NGTCAGAACCGATACGGACTTCCACCAGAGTTTCCTCTGGCTTCATCCTG	12	0.3	No Hit
NCTGTCACCTCTGTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCG	12	0.3	No Hit
NCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAACTAGATC	12	0.3	No Hit
NTAGTTGGATGTGGGTCAGTCGATCCTAAGGTACTGGCGAACGCCTTGTA	12	0.3	No Hit
NCTTTTATGGGGTCTCATGAGCGAGAAGTTAGGCACCTTAACTCCACGTT	12	0.3	No Hit
NTGCAGAGGTTGAGCAGTTGGCAAACGACCCGAAAGATGGTGAACTATGC	12	0.3	No Hit
NTATTAACCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGCTTTTC	12	0.3	No Hit
NACCAGAAAGCGTCCGCAAGCAAGCACAATCACTAGTCCGCCATCAACCC	11	0.27499999999999997	No Hit
NCCAACTACTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAAGGATC	11	0.27499999999999997	No Hit
NTCCTTAGCGGATACCGACTTCCATGGCCACCGTCCTGCTGTCAATATCA	11	0.27499999999999997	No Hit
NCTTAGCGGATACCGACTTCCATGGCCACCGTCCTGCTGTCAATATCAAC	11	0.27499999999999997	No Hit
NACCTATACTCCGCCGTTGCGACATGTGCGTTGTCTAGCGCCAGGTCGTA	11	0.27499999999999997	No Hit
NTCTTTGAGCGACTAACCAAGTTCGAATGCGTTACCGCGCTTGTGCTCCG	10	0.25	No Hit
NGGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGGAGCACA	10	0.25	No Hit
NTACATGGGCAGAAATCAGTGACAGTCAACGTCCGCAAGGACCATCTGTC	10	0.25	No Hit
NTCTTTGGCTGCTTCGAAATGTGGCGTATAGGTGTAAGTTTCCAGCAGTG	10	0.25	No Hit
NTTCCAACCACTGGAGCCAATCCTTTTCCCGAAGTTACGGATCTAGTTTG	9	0.22499999999999998	No Hit
NTCCAAGCACACACCAGAAAGCGTCCGCAAGCAAGCACAATCACTAGTCC	9	0.22499999999999998	No Hit
NTTTCTGTGGTGTCTCGTGCTCTTTGAACGGCCCTTAAAACACCAAGGGA	9	0.22499999999999998	No Hit
NATAAAAGGTGTTGGTTGATATTGACAGCAGGACGGTGGCCATGGAAGTC	9	0.22499999999999998	No Hit
NCTCTGCACAGTCACAAGTGACACGCACAGGGCCGTGGTGCGCTGCACTC	9	0.22499999999999998	No Hit
NGTTGATATTGACAGCAGGACGGTGGCCATGGAAGTCGGTATCCGCTAAG	9	0.22499999999999998	No Hit
NAATAATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGGGAA	9	0.22499999999999998	No Hit
NACTTGGACCTCGCATTCACGTTGACAGTTCACTAAAGAAACTGCAACTT	8	0.2	No Hit
NCTATACCCAAGTCTATCGATCGATTTGCACGTCAGAACCGATACGGACT	8	0.2	No Hit
NCTTGTGACTGTGCAGAGGTTGAGCAGTTGGCAAACGACCCGAAAGATGG	8	0.2	No Hit
NCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGCGATCAGGA	8	0.2	No Hit
NCCCAAGTCTATCGATCGATTTGCACGTCAGAACCGATACGGACTTCCAC	8	0.2	No Hit
NCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAAT	8	0.2	No Hit
NCGTAATGGTAGTAGCAAATATTCAAGTTCGATCCTTGAAGACTGAAGTG	8	0.2	No Hit
NCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGCTTTTCGCCACCG	8	0.2	No Hit
NTACTTGTTCGCTATCGCAATCGAGTCGATATTTAGCCTTAGATGAAGTT	8	0.2	No Hit
NGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGGAGCACAA	8	0.2	No Hit
NTTATATTCGGTAAAGCTAATGATTAGAGGCCTTGGGGACGTAATGTCCT	7	0.17500000000000002	No Hit
NCTTAGGATCGACTGACCCACATCCAACTACTGTTCACGTGGAACCCTTC	7	0.17500000000000002	No Hit
NCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAA	7	0.17500000000000002	No Hit
NTGTTGCCCATCTCGCAAGAGAAGGTGTCTTGCTGGCGGTAGTGGGTTCG	7	0.17500000000000002	No Hit
NCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACC	7	0.17500000000000002	No Hit
NTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACC	7	0.17500000000000002	No Hit
NCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCT	7	0.17500000000000002	No Hit
NATTAACCCTATTCCCTTTCGACAAGCGGGGACTAAAAGCAAGCTTTTCG	7	0.17500000000000002	No Hit
NACTAGTCCGCCATCAACCCGAAAGTATCAAGCAGACAGAACTGCGCCGG	7	0.17500000000000002	No Hit
NTACATGGGTCTCAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAG	7	0.17500000000000002	No Hit
NTTCGGAGCACAAGCGCGGTAACGCATTCGAACTTGGTTAGTCGCTCAAA	7	0.17500000000000002	No Hit
NCGCTAGTGTGTGTGACGTCGGTGTGAAAGTCGACGACGTTTCCGACCCG	7	0.17500000000000002	No Hit
NTCATAGCCTTCAACGCAACCGCCACGACCTTCCTACTCGTTACGACCTG	7	0.17500000000000002	No Hit
NTTCTGGATAGCGGCACTGTTGGTTCGCTTGCTCCTTGGAGTCGGGTTGC	7	0.17500000000000002	No Hit
NTCCTATGCTACGCCGACGGCGTTGGCTGCTCGTTCTAGCCCGACAGTGT	7	0.17500000000000002	No Hit
NTTCAAGAGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGTTGATGTT	7	0.17500000000000002	No Hit
NTTAAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTT	7	0.17500000000000002	No Hit
NCACACACTAGCGTAACTAAACGCTAGCCGCCACGAACCCACTACCGCCA	7	0.17500000000000002	No Hit
NACTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCAAGCA	7	0.17500000000000002	No Hit
NGAATAGGGTTAATATTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGG	7	0.17500000000000002	No Hit
NTGGTGTCTCGTGCTCTTTGAACGGCCCTTAAAACACCAAGGGAGGCTAT	7	0.17500000000000002	No Hit
NTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGC	7	0.17500000000000002	No Hit
NCTTAACTCCACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAA	6	0.15	No Hit
NGTATCAACGCAGAGTACATGGGCAGAAATCAGTGACAGTCAACGTCCGC	6	0.15	No Hit
NAGTATAGGTCTCTCGCTTAAGCGCCATCCATTTTCAGGGCTAGTTGATT	6	0.15	No Hit
NCGTCACACACACTAGCGTAACTAAACGCTAGCCGCCACGAACCCACTAC	6	0.15	No Hit
NTGTTAAAACCTTGCGGCGAAATGAAAGTAAAGGTCAGTCTCGAATTGGC	6	0.15	No Hit
NTCAATATCAACCAACACCTTTTATGGGGTCTCATGAGCGAGAAGTTAGG	6	0.15	No Hit
NCTCTGTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAG	6	0.15	No Hit
NTTGTTTAGATGAGCGATCAGGATACTAGAACCGAGGTCCAAGCACACAC	6	0.15	No Hit
NTTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTAATGCGGATATCGG	6	0.15	No Hit
NCATAGGAAACGACACCCGGCAACGATCACAACTAAGCGACCAGTCACCA	6	0.15	No Hit
NCCCGAAAGTATCAAGCAGACAGAACTGCGCCGGACAAGCATCAACCAAC	6	0.15	No Hit
NCTTGGAGACCTAATGCGGATATCGGTACGATTGAGTGCAAATTAATAGC	6	0.15	No Hit
NTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGG	6	0.15	No Hit
NTCAACGTGAATGCGAGGTCCAAGTGGGCCATTTTTGGTAAGCAGAACTG	6	0.15	No Hit
NCCTTGTATCATCGGTGGCGAAAAGCTTGCTTTTAGTCCCCGCTTGTCGA	6	0.15	No Hit
NTCAAAGACCGAGCTAGAGTTTTCTTCTCTAGTTAAGGAACGGACTCCCT	6	0.15	No Hit
NTATGAGCGTAGGCTCGGCTGGAGCTTCCGTCAGTGCAGATCGTAATGGT	6	0.15	No Hit
NTTCAAAGAGCACGAGACACCACAGAAACCGCGGTGCTTTTCGGAAACAA	6	0.15	No Hit
NGCCAGTACCTTAGGATCGACTGACCCACATCCAACTACTGTTCACGTGG	6	0.15	No Hit
NTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTCAAAGAGCACGAGACAC	6	0.15	No Hit
NTCAACGTCCGCAAGGACCATCTGTCACCTCTGTTTTAATTAGACAGTCG	6	0.15	No Hit
NCCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACC	6	0.15	No Hit
NTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTAATGCGGATATC	6	0.15	No Hit
NTTCCAGGGAGTCCGTTCCTTAACTAGAGAAGAAAACTCTAGCTCGGTCT	6	0.15	No Hit
NTCCTTACGATTGAGGCCATAAACCAGAGAGGGTGCGAGCCCCGTTCTGG	6	0.15	No Hit
NGATAGCTGGATCTCAGGCAGTTATATTCGGTAAAGCTAATGATTAGAGG	6	0.15	No Hit
NAGTTAAGGTGCCTAACTTCTCGCTCATGAGACCCCATAAAAGGTGTTGG	6	0.15	No Hit
NCGTTGAAGGCTATGAGCGTAGGCTCGGCTGGAGCTTCCGTCAGTGCAGA	5	0.125	No Hit
NTTGAGGACATTACGTCCCCAAGGCCTCTAATCATTAGCTTTACCGAATA	5	0.125	No Hit
NTTCTGACGTGCAAATCGATCGATAGACTTGGGTATAGGGGCGAAAGACT	5	0.125	No Hit
NTTTCAATGGATATGAAGTTGCAGTTTCTTTAGTGAACTGTCAACGTGAA	5	0.125	No Hit
NTCAGTGACAGTCAACGTCCGCAAGGACCATCTGTCACCTCTGTTTTAAT	5	0.125	No Hit
NTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAAGGATCGAACTTGAAT	5	0.125	No Hit
NATATGAAGTTGCAGTTTCTTTAGTGAACTGTCAACGTGAATGCGAGGTC	5	0.125	No Hit
NTATCATCGGTGGCGAAAAGCTTGCTTTTAGTCCCCGCTTGTCGAAAGGG	5	0.125	No Hit
NCTTAGTTGTGATCGTTGCCGGGTGTCGTTTCCTATGCTACGCCGACGGC	5	0.125	No Hit
NGACATTACGTCCCCAAGGCCTCTAATCATTAGCTTTACCGAATATAACT	5	0.125	No Hit
NCCTTAGTAACGGCGAGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCA	5	0.125	No Hit
NCCTAAAGTGGGTGATAAACTTCATCTAAGGCTAAATATCGACTCGATTG	5	0.125	No Hit
NACTATGCCTGAGCAGGATGAAGCCAGAGGAAACTCTGGTGGAAGTCCGT	5	0.125	No Hit
NCTAGTAGCTGGTTCCTTCCGAAGTTTCCCTCAGGATAGCTGGATCTCAG	5	0.125	No Hit
NTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACCGCGGTGCTTT	5	0.125	No Hit
NTTTTAAGGGCCGTTCAAAGAGCACGAGACACCACAGAAACCGCGGTGCT	5	0.125	No Hit
NTCACAAGTGACACGCACAGGGCCGTGGTGCGCTGCACTCCGAAGAACAC	5	0.125	No Hit
NAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGT	5	0.125	No Hit
NTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCG	5	0.125	No Hit
NGTGTGTGTGACGTCGGTGTGAAAGTCGACGACGTTTCCGACCCGTCTTG	5	0.125	No Hit
NACTTGGTTAGTCGCTCAAAGACCGAGCTAGAGTTTTCTTCTCTAGTTAA	5	0.125	No Hit
NTCCTAGTGTATCTCGTTATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC	5	0.125	No Hit
NACTAGATCCGTAACTTCGGGAAAAGGATTGGCTCCAGTGGTTGGAACGG	5	0.125	No Hit
NTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCAAGCACA	5	0.125	No Hit
NTGATACAAGGCGTTCGCCAGTACCTTAGGATCGACTGACCCACATCCAA	5	0.125	No Hit
NATGCAAAGATTGTGTTCTTCGGAGCACAAGCGCGGTAACGCATTCGAAC	5	0.125	No Hit
NCCTAACTGAGATGCAAAGATTGTGTTCTTCGGAGCACAAGCGCGGTAAC	5	0.125	No Hit
NTCGAAAGGGAATAGGGTTAATATTCCCTAACTGAGATGCAAAGATTGTG	5	0.125	No Hit
NCCTATTCTCAAACTTTCAATGGATATGAAGTTGCAGTTTCTTTAGTGAA	5	0.125	No Hit
NAGTTGATGTTGCTTGGAGACAAGCTTGGTGACTGGTCGCTTAGTTGTGA	5	0.125	No Hit
NACTAAAAGCAAGCTTTTCGCCACCGATGATACAAGGCGTTCGCCAGTAC	5	0.125	No Hit
NATCCAACTACTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAAGGA	5	0.125	No Hit
NTCCCTAACTGAGATGCAAAGATTGTGTTCTTCGGAGCACAAGCGCGGTA	5	0.125	No Hit
NCCCTTAAAACACCAAGGGAGGCTATTAATTTGCACTCAATCGTACCGAT	5	0.125	No Hit
NTCTTGAACTCTCTCTTCAAAGTCCTTTGCAACTTTCCCTCACGGTACTT	5	0.125	No Hit
NGTTAAGGAACGGACTCCCTGGAATTGGTTCAGCCAGAGATGGGGACGTT	5	0.125	No Hit
NCATAAAAGGTGTTGGTTGATATTGACAGCAGGACGGTGGCCATGGAAGT	5	0.125	No Hit
NCAGGACGGTGGCCATGGAAGTCGGTATCCGCTAAGGAGTGTGTAACAAC	5	0.125	No Hit
NAGCTAGAGTTTTCTTCTCTAGTTAAGGAACGGACTCCCTGGAATTGGTT	5	0.125	No Hit
NCAGAGAGGGTGCGAGCCCCGTTCTGGATAGCGGCACTGTTGGTTCGCTT	5	0.125	No Hit
NCTCTGGCTGAACCAATTCCAGGGAGTCCGTTCCTTAACTAGAGAAGAAA	5	0.125	No Hit
NCAGAAAGCGTCCGCAAGCAAGCACAATCACTAGTCCGCCATCAACCCGA	5	0.125	No Hit
NAACTGGCGCTGTGGGATGAACCAAACGTGGAGTTAAGGTGCCTAACTTC	5	0.125	No Hit
NACAAGTACCGTGAGGGAAAGTTGCAAAGGACTTTGAAGAGAGAGTTCAA	5	0.125	No Hit
NGGCCGTGGTGCGCTGCACTCCGAAGAACACAGATCCCACGTCGGCCAAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0125	0.0
32-33	0.0	0.0	0.0	0.11249999999999999	0.0
34-35	0.0	0.0	0.0	0.2375	0.0
36-37	0.0	0.0	0.0	0.275	0.0
38-39	0.0	0.0	0.0	0.45	0.0
40-41	0.0	0.0	0.0	0.675	0.0
42-43	0.0	0.0	0.0	0.7875	0.0
44-45	0.0	0.0	0.0	0.875	0.0
46-47	0.0	0.0	0.0	1.0375	0.0
48-49	0.0	0.0	0.0	1.1875	0.0
50-51	0.0	0.0	0.0	1.3375	0.0
52-53	0.0	0.0	0.0	1.475	0.0
54-55	0.0	0.0	0.0	1.625	0.0
56-57	0.0	0.0	0.0	1.7374999999999998	0.0
58-59	0.0	0.0	0.0	1.875	0.0
60-61	0.0	0.0	0.0	2.05	0.0
62-63	0.0	0.0	0.0	2.2	0.0
64-65	0.0	0.0	0.0	2.3375	0.0
66-67	0.0	0.0	0.0	2.45	0.0
68-69	0.0	0.0	0.0	2.575	0.0
70-71	0.0	0.0	0.0	2.75	0.0
72-73	0.0	0.0	0.0	2.9125	0.0
74-75	0.0	0.0	0.0	3.15	0.0
76-77	0.0	0.0	0.0	3.325	0.0
78-79	0.0	0.0	0.0	3.4625000000000004	0.0
80-81	0.0	0.0	0.0	3.5999999999999996	0.0
82-83	0.0	0.0	0.0	3.8125	0.0
84-85	0.0	0.0	0.0	3.95	0.0
86-87	0.0	0.0	0.0	4.0875	0.0
88-89	0.0	0.0	0.0	4.2375	0.0
90-91	0.0	0.0	0.0	4.4625	0.0
92-93	0.0	0.0	0.0	4.7625	0.0
94-95	0.0	0.0	0.0	5.0	0.0
96-97	0.0	0.0	0.0	5.2375	0.0
98-99	0.0	0.0	0.0	5.5	0.0
100-101	0.0	0.0	0.0	5.8375	0.0
102-103	0.0	0.0	0.0	5.9375	0.0
104-105	0.0	0.0	0.0	6.1625	0.0
106-107	0.0	0.0	0.0	6.4125	0.0
108-109	0.0	0.0	0.0	6.625	0.0
110-111	0.0	0.0	0.0	6.875	0.0
112-113	0.0	0.0	0.0	7.0375	0.0
114-115	0.0	0.0	0.0	7.275	0.0
116-117	0.0	0.0	0.0	7.575	0.0
118-119	0.0	0.0	0.0	7.8125	0.0
120-121	0.0	0.0	0.0	8.0125	0.0
122-123	0.0	0.0	0.0	8.149999999999999	0.0
124-125	0.0	0.0	0.0	8.3625	0.0
126-127	0.0	0.0	0.0	8.5625	0.0
128-129	0.0	0.0	0.0	8.8375	0.0
130-131	0.0	0.0	0.0	9.0875	0.0
132-133	0.0	0.0	0.0	9.3	0.0
134-135	0.0	0.0	0.0	9.524999999999999	0.0
136-137	0.0	0.0	0.0	9.8875	0.0
138	0.0	0.0	0.0	10.125	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAAAA	80	3.6472738E-7	17.875	135-139
>>END_MODULE
Read 1363357 spots for SRR13919969.sra
Written 1363357 spots for SRR13919969.sra
Read 1363357 spots for SRR13919969.sra
Written 1363357 spots for SRR13919969.sra
Read 1363357 spots for SRR13919969.sra
Written 1363357 spots for SRR13919969.sra
Read 1363357 spots for SRR13919969.sra
Written 1363357 spots for SRR13919969.sra
Read 1363357 spots for SRR13919969.sra
Written 1363357 spots for SRR13919969.sra
Read 1363357 spots for SRR13919969.sra
Written 1363357 spots for SRR13919969.sra
Read 1363357 spots for SRR13919969.sra
Written 1363357 spots for SRR13919969.sra
Read 1363357 spots for SRR13919969.sra
Written 1363357 spots for SRR13919969.sra
Read 1363357 spots for SRR13919969.sra
Written 1363357 spots for SRR13919969.sra
Read 1363359 spots for SRR13919969.sra
Written 1363359 spots for SRR13919969.sra
Read 1363357 spots for SRR13919969.sra
Written 1363357 spots for SRR13919969.sra
Read 1363357 spots for SRR13919969.sra
Written 1363357 spots for SRR13919969.sra
Read 1363357 spots for SRR13919969.sra
Written 1363357 spots for SRR13919969.sra
Read 1363357 spots for SRR13919969.sra
Written 1363357 spots for SRR13919969.sra
Read 1363357 spots for SRR13919969.sra
Written 1363357 spots for SRR13919969.sra
Read 1363357 spots for SRR13919969.sra
Written 1363357 spots for SRR13919969.sra
SRR ids: ['SRR13919969.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_4r_io563
SRR13919969.sra spots: 21813714
blocks: [[1, 1363357], [1363358, 2726714], [2726715, 4090071], [4090072, 5453428], [5453429, 6816785], [6816786, 8180142], [8180143, 9543499], [9543500, 10906856], [10906857, 12270213], [12270214, 13633570], [13633571, 14996927], [14996928, 16360284], [16360285, 17723641], [17723642, 19086998], [19086999, 20450355], [20450356, 21813714]]
SRR13919969 file size 8010732
SRR13919969 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 16 -o SRR13919969 SRR13919969_1.fastq SRR13919969_2.fastq
Input file:	SRR13919969_1.fastq
Paired file:	SRR13919969_2.fastq
trimmed:	SRR13919969-trimmed-pair1.fastq, SRR13919969-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	16
Wed Dec  1 22:09:21 2021 >> started

Wed Dec  1 22:11:52 2021 >> done (151.530s)
21813714 read pairs processed; of these:
   33963 ( 0.16%) short read pairs filtered out after trimming by size control
   10674 ( 0.05%) empty read pairs filtered out after trimming by size control
21769077 (99.80%) read pairs available; of these:
 1254913 ( 5.76%) trimmed read pairs available after processing
20514164 (94.24%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 82	       5	  0.00%
 83	      81	  0.00%
 84	    2789	  0.01%
 85	    3716	  0.02%
 86	    2759	  0.01%
 87	    4855	  0.02%
 88	    4386	  0.02%
 89	    1605	  0.01%
 90	    2988	  0.01%
 91	    3130	  0.01%
 92	    2692	  0.01%
 93	    3791	  0.02%
 94	    1333	  0.01%
 95	    2775	  0.01%
 96	    1410	  0.01%
 97	    2963	  0.01%
 98	    1657	  0.01%
 99	    2635	  0.01%
100	    1951	  0.01%
101	    1405	  0.01%
102	     120	  0.00%
103	     647	  0.00%
104	     942	  0.00%
105	     930	  0.00%
106	     488	  0.00%
107	     641	  0.00%
108	       5	  0.00%
109	     616	  0.00%
110	      72	  0.00%
111	    1027	  0.00%
112	     152	  0.00%
113	       0	  0.00%
114	       0	  0.00%
115	      14	  0.00%
116	       0	  0.00%
117	      11	  0.00%
118	      12	  0.00%
119	     996	  0.00%
120	      48	  0.00%
121	      75	  0.00%
122	      64	  0.00%
123	       0	  0.00%
124	     309	  0.00%
125	      14	  0.00%
126	     102	  0.00%
127	      14	  0.00%
128	      32	  0.00%
129	     534	  0.00%
130	      41	  0.00%
131	     156	  0.00%
132	      31	  0.00%
133	     936	  0.00%
134	      48	  0.00%
135	    2110	  0.01%
136	    1580	  0.01%
137	    1829	  0.01%
138	     961	  0.00%
139	      16	  0.00%
140	    1037	  0.00%
141	     150	  0.00%
142	     765	  0.00%
143	     203	  0.00%
144	     530	  0.00%
145	    1567	  0.01%
146	    6586	  0.03%
147	   20020	  0.09%
148	   89278	  0.41%
149	 1070278	  4.92%
150	20514164	 94.24%
21769077 reads passed initial QC


criterion=sequence-density
sequence-density=8.49
sequence-density-rank=1
fanout-score=45.83
fanout-score-rank=1
prefix-density=8.90
prefix-fanout=43.7
sequence=CTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA


criterion=fanout-score
sequence-density=8.49
sequence-density-rank=1
fanout-score=45.83
fanout-score-rank=1
prefix-density=8.90
prefix-fanout=43.7
sequence=CTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA


criterion=sequence-density
sequence-density=8.62
sequence-density-rank=1
fanout-score=45.86
fanout-score-rank=1
prefix-density=9.03
prefix-fanout=43.8
sequence=CTGTCTCTTATACACATCTGACGCTGCCGACGATCGCATAAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAA


criterion=fanout-score
sequence-density=8.62
sequence-density-rank=1
fanout-score=45.86
fanout-score-rank=1
prefix-density=9.03
prefix-fanout=43.8
sequence=CTGTCTCTTATACACATCTGACGCTGCCGACGATCGCATAAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 818: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 819: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 16 -x CTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA -y CTGTCTCTTATACACATCTGACGCTGCCGACGATCGCATAAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAA -o SRR13919969 SRR13919969_1.fastq SRR13919969_2.fastq
Input file:	SRR13919969_1.fastq
Paired file:	SRR13919969_2.fastq
trimmed:	SRR13919969-trimmed-pair1.fastq, SRR13919969-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCT
-- paired 3' end adapter sequence (-y):	CTGTCTCTTATACACATCTGACGCTGCCGACGATCGCATAAGTGTAGATCTCGGTGGTCGCCGT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	16
Wed Dec  1 22:22:52 2021 >> started

Wed Dec  1 22:25:00 2021 >> done (127.936s)
16931504 read pairs processed; of these:
      47 ( 0.00%) short read pairs filtered out after trimming by size control
       1 ( 0.00%) empty read pairs filtered out after trimming by size control
16931456 (100.00%) read pairs available; of these:
 1711825 (10.11%) trimmed read pairs available after processing
15219631 (89.89%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      10	  0.00%
 19	       8	  0.00%
 20	       8	  0.00%
 21	      16	  0.00%
 22	      24	  0.00%
 23	      36	  0.00%
 24	      27	  0.00%
 25	      41	  0.00%
 26	      64	  0.00%
 27	     362	  0.00%
 28	     878	  0.01%
 29	    1763	  0.01%
 30	    2810	  0.02%
 31	    3500	  0.02%
 32	    2564	  0.02%
 33	    2905	  0.02%
 34	    3356	  0.02%
 35	    3707	  0.02%
 36	    7000	  0.04%
 37	    7855	  0.05%
 38	    8420	  0.05%
 39	   10277	  0.06%
 40	    7844	  0.05%
 41	    8738	  0.05%
 42	    7370	  0.04%
 43	    8049	  0.05%
 44	    8427	  0.05%
 45	    8429	  0.05%
 46	    9247	  0.05%
 47	    9253	  0.05%
 48	    9237	  0.05%
 49	    9737	  0.06%
 50	    9277	  0.05%
 51	    9479	  0.06%
 52	    8171	  0.05%
 53	    9828	  0.06%
 54	    9107	  0.05%
 55	   10454	  0.06%
 56	   10518	  0.06%
 57	    9203	  0.05%
 58	   10550	  0.06%
 59	   10469	  0.06%
 60	   10831	  0.06%
 61	   10771	  0.06%
 62	    9958	  0.06%
 63	   10590	  0.06%
 64	   10228	  0.06%
 65	   10239	  0.06%
 66	   11839	  0.07%
 67	   10552	  0.06%
 68	   12011	  0.07%
 69	   10361	  0.06%
 70	   11858	  0.07%
 71	   10453	  0.06%
 72	   11214	  0.07%
 73	   10691	  0.06%
 74	   11188	  0.07%
 75	   10434	  0.06%
 76	   11863	  0.07%
 77	   11688	  0.07%
 78	   14645	  0.09%
 79	   10754	  0.06%
 80	   14517	  0.09%
 81	   11628	  0.07%
 82	   13358	  0.08%
 83	   13029	  0.08%
 84	   13411	  0.08%
 85	   15605	  0.09%
 86	   14373	  0.08%
 87	   15733	  0.09%
 88	   16268	  0.10%
 89	   14460	  0.09%
 90	   17362	  0.10%
 91	   18004	  0.11%
 92	   16575	  0.10%
 93	   18005	  0.11%
 94	   14168	  0.08%
 95	   15626	  0.09%
 96	   14557	  0.09%
 97	   16714	  0.10%
 98	   15116	  0.09%
 99	   18407	  0.11%
100	   16145	  0.10%
101	   16895	  0.10%
102	   15428	  0.09%
103	   14730	  0.09%
104	   16734	  0.10%
105	   16761	  0.10%
106	   14514	  0.09%
107	   16804	  0.10%
108	   14331	  0.08%
109	   17412	  0.10%
110	   18131	  0.11%
111	   17852	  0.11%
112	   18833	  0.11%
113	   16053	  0.09%
114	   17907	  0.11%
115	   17834	  0.11%
116	   17977	  0.11%
117	   17187	  0.10%
118	   17397	  0.10%
119	   19315	  0.11%
120	   18953	  0.11%
121	   21294	  0.13%
122	   20316	  0.12%
123	   18781	  0.11%
124	   19669	  0.12%
125	   16400	  0.10%
126	   19292	  0.11%
127	   18295	  0.11%
128	   20522	  0.12%
129	   22356	  0.13%
130	   21597	  0.13%
131	   22106	  0.13%
132	   19059	  0.11%
133	   20815	  0.12%
134	   21019	  0.12%
135	   24646	  0.15%
136	   23100	  0.14%
137	   24485	  0.14%
138	   21589	  0.13%
139	   20975	  0.12%
140	   24584	  0.15%
141	   22383	  0.13%
142	   25019	  0.15%
143	   22660	  0.13%
144	   22477	  0.13%
145	   27497	  0.16%
146	   28909	  0.17%
147	   39178	  0.23%
148	   89045	  0.53%
149	  797990	  4.71%
150	14310103	 84.52%


criterion=sequence-density
sequence-density=4.52
sequence-density-rank=1
fanout-score=2.05
fanout-score-rank=33
prefix-density=4.53
prefix-fanout=2.0
sequence=CTCCGAAGAACACA


criterion=fanout-score
sequence-density=0.08
sequence-density-rank=31
fanout-score=32.30
fanout-score-rank=1
prefix-density=2.66
prefix-fanout=1.0
sequence=GTACATGGGGAA


criterion=sequence-density
sequence-density=4.21
sequence-density-rank=1
fanout-score=2.07
fanout-score-rank=33
prefix-density=4.25
prefix-fanout=2.1
sequence=CTCCGAAGAACACA


criterion=fanout-score
sequence-density=0.08
sequence-density-rank=31
fanout-score=34.30
fanout-score-rank=1
prefix-density=2.63
prefix-fanout=1.0
sequence=GTACATGGGGAA
SRR13919969 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Dec 01 22:32:49
                             Started mapping on |	Dec 01 22:32:49
                                    Finished on |	Dec 01 22:46:08
       Mapping speed, Million of reads per hour |	98.08

                          Number of input reads |	21769029
                      Average input read length |	288
                                    UNIQUE READS:
                   Uniquely mapped reads number |	17820348
                        Uniquely mapped reads % |	81.86%
                          Average mapped length |	291.68
                       Number of splices: Total |	2299839
            Number of splices: Annotated (sjdb) |	2215298
                       Number of splices: GT/AG |	2272414
                       Number of splices: GC/AG |	10597
                       Number of splices: AT/AC |	527
               Number of splices: Non-canonical |	16301
                      Mismatch rate per base, % |	0.37%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.41
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.16
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1413835
             % of reads mapped to multiple loci |	6.49%
        Number of reads mapped to too many loci |	10806
             % of reads mapped to too many loci |	0.05%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	11.55%
                     % of reads unmapped: other |	0.05%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2538385	2538385	2538385
N_multimapping	1413835	1413835	1413835
N_noFeature	194363	9047833	8824252
N_ambiguous	281038	101212	99275
UnstrandedReadsAssigned:17344947 PositiveStrandReadsAssigned:8671303 NegativeStrandReadsAssigned:8896821
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR13919969 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 33,746
[index] number of k-mers: 28,927,460
[index] number of equivalence classes: 54,013
[quant] running in paired-end mode
[quant] will process pair 1: SRR13919969-trimmed-pair1.fastq
                             SRR13919969-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 21,769,029 reads, 2,856,080 reads pseudoaligned
[quant] estimated average fragment length: 299.919
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,145 rounds

  33747 SRR13919969.ke.tsv
  46739 SRR13919969.se.tsv
  80486 total
==> SRR13919969.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Y110A7A.10	1787	1488.08	283	49.5616
F27C8.1	1940	1641.08	37	5.87566
F07C3.7	1728	1429.08	23	4.19427
F52H2.2a	1739	1440.08	80.9671	14.6523
F52H2.2b	1840	1541.08	61.0329	10.321
T13A10.10a	1734	1435.08	1	0.181597
T13A10.10b	1598	1299.08	0	0
C55C2.5a	1840	1541.08	90.447	15.2952
C55C2.5b	1606	1307.08	5.37721	1.07211

==> SRR13919969.se.tsv <==
WBGene00197333	0
WBGene00198386	0
WBGene00015153	0
WBGene00002061	92
WBGene00255704	8
WBGene00235314	34
WBGene00001177	772
WBGene00169236	0
WBGene00219784	0
WBGene00015152	4
SRR13919969 completed mapping pipeline successfully
