Starting /dee2/code/volunteer_pipeline.sh SRR8447878
    current disk space = 94037756
    free memory = 63022420 
SRR8447878 SRAfilesize
d899a0adcdba1b6f07ad9cd3914d3551  SRR8447878.sra
SRR8447878.sra file validated
SRR8447878 is single end
SRR8447878 is conventional basespace
SRR8447878 read1 length is 75 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8447878_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	75
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.4465	37.0	37.0	37.0	37.0	37.0
2	36.278	37.0	37.0	37.0	37.0	37.0
3	36.2405	37.0	37.0	37.0	37.0	37.0
4	36.433	37.0	37.0	37.0	37.0	37.0
5	36.1635	37.0	37.0	37.0	37.0	37.0
6	36.366	37.0	37.0	37.0	37.0	37.0
7	36.447	37.0	37.0	37.0	37.0	37.0
8	36.353	37.0	37.0	37.0	37.0	37.0
9	36.42	37.0	37.0	37.0	37.0	37.0
10	36.191	37.0	37.0	37.0	37.0	37.0
11	36.224	37.0	37.0	37.0	37.0	37.0
12	36.334	37.0	37.0	37.0	37.0	37.0
13	36.3475	37.0	37.0	37.0	37.0	37.0
14	36.342	37.0	37.0	37.0	37.0	37.0
15	36.3595	37.0	37.0	37.0	37.0	37.0
16	36.405	37.0	37.0	37.0	37.0	37.0
17	36.3805	37.0	37.0	37.0	37.0	37.0
18	36.26	37.0	37.0	37.0	37.0	37.0
19	36.151	37.0	37.0	37.0	37.0	37.0
20	36.2895	37.0	37.0	37.0	37.0	37.0
21	36.3535	37.0	37.0	37.0	37.0	37.0
22	36.418	37.0	37.0	37.0	37.0	37.0
23	36.1505	37.0	37.0	37.0	37.0	37.0
24	36.355	37.0	37.0	37.0	37.0	37.0
25	36.391	37.0	37.0	37.0	37.0	37.0
26	36.217	37.0	37.0	37.0	37.0	37.0
27	36.186	37.0	37.0	37.0	37.0	37.0
28	36.097	37.0	37.0	37.0	37.0	37.0
29	36.187	37.0	37.0	37.0	37.0	37.0
30	36.241	37.0	37.0	37.0	37.0	37.0
31	36.241	37.0	37.0	37.0	37.0	37.0
32	36.297	37.0	37.0	37.0	37.0	37.0
33	36.187	37.0	37.0	37.0	37.0	37.0
34	36.16	37.0	37.0	37.0	37.0	37.0
35	36.336	37.0	37.0	37.0	37.0	37.0
36	36.239	37.0	37.0	37.0	37.0	37.0
37	36.3725	37.0	37.0	37.0	37.0	37.0
38	36.174	37.0	37.0	37.0	37.0	37.0
39	36.1875	37.0	37.0	37.0	37.0	37.0
40	35.918	37.0	37.0	37.0	37.0	37.0
41	35.973	37.0	37.0	37.0	37.0	37.0
42	35.8585	37.0	37.0	37.0	37.0	37.0
43	34.1385	37.0	37.0	37.0	25.0	37.0
44	35.757	37.0	37.0	37.0	37.0	37.0
45	35.9995	37.0	37.0	37.0	37.0	37.0
46	35.756	37.0	37.0	37.0	37.0	37.0
47	35.6495	37.0	37.0	37.0	37.0	37.0
48	36.1045	37.0	37.0	37.0	37.0	37.0
49	35.978	37.0	37.0	37.0	37.0	37.0
50	35.745	37.0	37.0	37.0	37.0	37.0
51	35.9335	37.0	37.0	37.0	37.0	37.0
52	36.159	37.0	37.0	37.0	37.0	37.0
53	35.8415	37.0	37.0	37.0	37.0	37.0
54	35.584	37.0	37.0	37.0	37.0	37.0
55	35.4325	37.0	37.0	37.0	37.0	37.0
56	35.567	37.0	37.0	37.0	37.0	37.0
57	34.82	37.0	37.0	37.0	25.0	37.0
58	34.6735	37.0	37.0	37.0	25.0	37.0
59	35.6075	37.0	37.0	37.0	37.0	37.0
60	33.853	37.0	37.0	37.0	25.0	37.0
61	34.402	37.0	37.0	37.0	25.0	37.0
62	34.516	37.0	37.0	37.0	25.0	37.0
63	34.4555	37.0	37.0	37.0	25.0	37.0
64	32.062	37.0	37.0	37.0	11.0	37.0
65	30.7975	37.0	25.0	37.0	11.0	37.0
66	30.869	37.0	25.0	37.0	11.0	37.0
67	30.885	37.0	25.0	37.0	11.0	37.0
68	30.1605	37.0	25.0	37.0	11.0	37.0
69	30.974	37.0	25.0	37.0	11.0	37.0
70	32.3905	37.0	37.0	37.0	11.0	37.0
71	33.161	37.0	37.0	37.0	25.0	37.0
72	34.079	37.0	37.0	37.0	25.0	37.0
73	34.22	37.0	37.0	37.0	25.0	37.0
74	33.9505	37.0	37.0	37.0	25.0	37.0
75	34.05	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	4.0
24	1.0
25	11.0
26	11.0
27	30.0
28	34.0
29	31.0
30	56.0
31	96.0
32	177.0
33	234.0
34	233.0
35	750.0
36	2308.0
37	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	7.95	10.825	69.675	11.55
2	31.125000000000004	29.599999999999998	17.349999999999998	21.925
3	23.9	33.125	25.825	17.150000000000002
4	25.7	29.049999999999997	23.674999999999997	21.575
5	19.400000000000002	36.95	29.549999999999997	14.099999999999998
6	21.725	21.775	33.375	23.125
7	20.7	26.0	34.1	19.2
8	21.75	25.05	28.1	25.1
9	16.925	21.224999999999998	41.475	20.375
10	13.8	27.675	28.000000000000004	30.525000000000002
11	25.7	27.375	29.825000000000003	17.1
12	32.375	17.875	29.575000000000003	20.175
13	28.875	20.8	30.25	20.075000000000003
14	18.575	25.4	35.449999999999996	20.575
15	29.975	22.400000000000002	27.1	20.525
16	19.925	19.925	27.875	32.275
17	22.575	21.6	27.625	28.199999999999996
18	23.599999999999998	28.575	24.6	23.225
19	21.349999999999998	27.025	25.85	25.775
20	30.7	19.475	34.375	15.45
21	32.574999999999996	25.025	21.525	20.875
22	20.075000000000003	28.849999999999998	35.925000000000004	15.15
23	26.075	23.724999999999998	35.575	14.625
24	38.875	14.799999999999999	24.325	22.0
25	37.425000000000004	20.1	31.474999999999998	11.0
26	25.900000000000002	32.574999999999996	23.625	17.9
27	26.0	31.2	25.924999999999997	16.875
28	24.375	31.125000000000004	37.9	6.6000000000000005
29	27.325	24.0	35.425000000000004	13.25
30	14.224999999999998	22.175	40.9	22.7
31	10.6	16.6	43.075	29.725
32	17.2	18.075	33.324999999999996	31.4
33	33.650000000000006	3.925	29.725	32.7
34	50.8	2.125	24.075	23.0
35	52.6	2.325	22.225	22.85
36	44.7	3.075	25.874999999999996	26.35
37	52.400000000000006	5.55	21.75	20.3
38	39.975	15.975	12.25	31.8
39	32.6	15.8	13.025	38.574999999999996
40	27.175	39.45	10.549999999999999	22.825
41	25.424999999999997	32.875	16.225	25.474999999999998
42	35.575	27.650000000000002	4.175	32.6
43	34.599999999999994	37.55	7.75	20.1
44	22.75	50.4	3.5000000000000004	23.35
45	32.975	45.074999999999996	4.8	17.150000000000002
46	22.775000000000002	31.55	19.900000000000002	25.775
47	17.150000000000002	37.5	20.349999999999998	25.0
48	12.25	26.025	20.7	41.025
49	15.7	26.825	14.249999999999998	43.225
50	12.3	28.825	16.1	42.775
51	20.775	16.25	19.6	43.375
52	21.625	15.875	25.8	36.7
53	15.6	23.325000000000003	22.125	38.95
54	18.4	28.425	13.575000000000001	39.6
55	22.875	18.15	23.075000000000003	35.9
56	35.099999999999994	15.625	21.2	28.075
57	31.45	16.175	11.25	41.125
58	23.325000000000003	14.2	22.275	40.2
59	32.9	19.075	21.025	27.0
60	22.85	24.7	14.274999999999999	38.175
61	17.05	21.075	25.324999999999996	36.55
62	10.875	30.375000000000004	33.775	24.975
63	13.675	23.549999999999997	32.1	30.675
64	6.225	30.349999999999998	26.450000000000003	36.975
65	8.649999999999999	29.475	31.45	30.425
66	14.85	23.05	24.95	37.15
67	23.674999999999997	18.4	31.275	26.650000000000002
68	23.45	13.225000000000001	39.875	23.45
69	26.674999999999997	19.575	30.55	23.200000000000003
70	34.075	11.125	36.375	18.425
71	39.85	11.825	26.575	21.75
72	29.575000000000003	12.7	24.875	32.85
73	33.0	9.55	24.825	32.625
74	40.625	6.5	26.200000000000003	26.674999999999997
75	29.425	2.825	32.65	35.099999999999994
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.5
23	0.5
24	0.0
25	1.0
26	1.0
27	0.0
28	0.0
29	0.5
30	1.5
31	1.0
32	0.0
33	0.0
34	0.5
35	1.0
36	1.0
37	2.0
38	2.0
39	2.0
40	3.0
41	9.0
42	30.5
43	46.0
44	46.0
45	71.5
46	120.0
47	223.0
48	303.0
49	407.5
50	568.5
51	585.0
52	545.0
53	520.0
54	446.5
55	387.0
56	376.0
57	312.0
58	168.5
59	57.0
60	25.0
61	19.0
62	10.0
63	4.5
64	2.0
65	1.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.19999999999999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.02564102564102	63.9
2	4.665242165242165	6.550000000000001
3	1.103988603988604	2.325
4	0.7834757834757835	2.1999999999999997
5	0.39173789173789175	1.375
6	0.3205128205128205	1.35
7	0.3561253561253561	1.7500000000000002
8	0.14245014245014245	0.8
9	0.14245014245014245	0.8999999999999999
>10	0.9259259259259258	10.75
>50	0.10683760683760685	5.1499999999999995
>100	0.03561253561253561	2.9499999999999997
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAGGGGGGGG	118	2.9499999999999997	RNA PCR Primer, Index 11 (100% over 63bp)
TCAGTCTTTTTCTCTCTCCTATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	81	2.025	RNA PCR Primer, Index 11 (100% over 53bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCGCGTATGCCGTCTTCTGCTTGAAAAGGGGGGGG	73	1.825	RNA PCR Primer, Index 11 (98% over 63bp)
TCACTGGGCTTTGTTTATCTCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	52	1.3	RNA PCR Primer, Index 11 (100% over 53bp)
CTAAGGAAATAGTAGCCGTGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	34	0.8500000000000001	RNA PCR Primer, Index 11 (100% over 53bp)
TGAGATCATTTTGAAAGCTGATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCG	33	0.8250000000000001	RNA PCR Primer, Index 11 (100% over 52bp)
TAATACTGTCAGGTAAAGATGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCG	26	0.65	RNA PCR Primer, Index 11 (100% over 52bp)
TCAGTCTTTTTCTCTCTCCTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTC	26	0.65	RNA PCR Primer, Index 11 (100% over 54bp)
TGTGATGTGACGTAGTGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTC	25	0.625	RNA PCR Primer, Index 11 (100% over 54bp)
TGTGATGTGACGTAGTGGAACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	23	0.575	RNA PCR Primer, Index 11 (100% over 53bp)
TGACTAGATCCACACTCATTAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	22	0.5499999999999999	RNA PCR Primer, Index 11 (100% over 53bp)
TATCACAGCCATTTTGACGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCG	19	0.475	RNA PCR Primer, Index 11 (100% over 52bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAATGGGGGGG	18	0.44999999999999996	RNA PCR Primer, Index 11 (100% over 63bp)
TGGACGGAGAACTGATAAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	15	0.375	RNA PCR Primer, Index 11 (100% over 53bp)
CTAAGGAAATAGTAGCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTC	15	0.375	RNA PCR Primer, Index 11 (100% over 54bp)
TAGCACCACATGATTCGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTC	15	0.375	RNA PCR Primer, Index 11 (100% over 54bp)
TGCTTGGACTACATATGGTTGAGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC	14	0.35000000000000003	RNA PCR Primer, Index 11 (100% over 50bp)
TAATACTGTCAGGTAAAGATGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCGCGTATGCCG	14	0.35000000000000003	RNA PCR Primer, Index 11 (98% over 52bp)
TAAGGAAATAGTAGCCGTGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTC	13	0.325	RNA PCR Primer, Index 11 (100% over 54bp)
CATCACAGTCTGAGTTCTTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	13	0.325	RNA PCR Primer, Index 11 (100% over 53bp)
TATTGCACACTTCCCGGCCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	12	0.3	RNA PCR Primer, Index 11 (100% over 53bp)
TATCACAGCCATTTTGACGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	11	0.27499999999999997	RNA PCR Primer, Index 11 (100% over 53bp)
TGAGTATTACATCAGGTACTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCG	11	0.27499999999999997	RNA PCR Primer, Index 11 (100% over 52bp)
TCAGTCTTTTTCTCTCTCCTATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCGCGTATGCCGT	11	0.27499999999999997	RNA PCR Primer, Index 11 (98% over 53bp)
TCTTTGGTATTCTAGCTGTAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	10	0.25	RNA PCR Primer, Index 11 (100% over 53bp)
ATTGTACTTCATCAGGTGCTCTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	10	0.25	RNA PCR Primer, Index 11 (100% over 51bp)
TGGACGGAGAACTGATAAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCGCGTATGCCGT	10	0.25	RNA PCR Primer, Index 11 (98% over 53bp)
TCTTTGGTGATTTTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	10	0.25	RNA PCR Primer, Index 11 (100% over 53bp)
CTAAGGAAATAGTAGCCGTGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCGCGTATGCCGT	10	0.25	RNA PCR Primer, Index 11 (98% over 53bp)
AATTGCACTAGTCCCGGCCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	10	0.25	RNA PCR Primer, Index 11 (100% over 53bp)
TAGGAACTTCATACCGTGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	9	0.22499999999999998	RNA PCR Primer, Index 11 (100% over 53bp)
CATCACAGTCTGAGTTCTTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCGCGTATGCCGT	9	0.22499999999999998	RNA PCR Primer, Index 11 (98% over 53bp)
TATTGCACATTCACCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	9	0.22499999999999998	RNA PCR Primer, Index 11 (100% over 53bp)
GTGCATTGTAGTCGCATTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTC	9	0.22499999999999998	RNA PCR Primer, Index 11 (100% over 54bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAACAGGGGGGGG	8	0.2	RNA PCR Primer, Index 11 (100% over 63bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCGGCTTGAAAAGGGGGGGG	8	0.2	RNA PCR Primer, Index 11 (98% over 63bp)
CATTGCACTTGTCCCGGCCTATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	8	0.2	RNA PCR Primer, Index 11 (100% over 53bp)
TCACTGGGCTTTGTTTATCTCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCGCGTATGCCGT	8	0.2	RNA PCR Primer, Index 11 (98% over 53bp)
TGAGATCATTTTGAAAGCTGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	7	0.17500000000000002	RNA PCR Primer, Index 11 (100% over 53bp)
TAGCACCACATGATTCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTCT	7	0.17500000000000002	RNA PCR Primer, Index 11 (100% over 55bp)
TGGACGGAGAACTGATAAGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTC	7	0.17500000000000002	RNA PCR Primer, Index 11 (100% over 54bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCGCGTATGCCGTCTTCTGCTTGAAACGGGGGGGG	7	0.17500000000000002	RNA PCR Primer, Index 11 (98% over 63bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAGGGGGGGGG	7	0.17500000000000002	RNA PCR Primer, Index 11 (100% over 63bp)
ATTGTACTTCATCAGGTGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTC	7	0.17500000000000002	RNA PCR Primer, Index 11 (100% over 54bp)
TGAGATCATTTTGAAAGCTGATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCGCGTATGCCG	7	0.17500000000000002	RNA PCR Primer, Index 11 (98% over 52bp)
TAGCCTCTACTAGGCTTTGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	7	0.17500000000000002	RNA PCR Primer, Index 11 (100% over 53bp)
TATCACAGCCATTTTGACGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCGCGTATGCCG	7	0.17500000000000002	RNA PCR Primer, Index 11 (98% over 52bp)
TCACTGGGCTTTGTTTATCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTC	7	0.17500000000000002	RNA PCR Primer, Index 11 (100% over 54bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCGCGTATGCCGTCTTCTGCTTGAAAATGGGGGGG	6	0.15	RNA PCR Primer, Index 11 (98% over 63bp)
ATTGTACTTCATCAGGTGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	6	0.15	RNA PCR Primer, Index 11 (100% over 53bp)
ATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTC	6	0.15	RNA PCR Primer, Index 11 (100% over 57bp)
TGTGATGTGACGTAGTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTCT	6	0.15	RNA PCR Primer, Index 11 (100% over 55bp)
TATTGCACACTTCCCGGCCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCGCGTATGCCGT	6	0.15	RNA PCR Primer, Index 11 (98% over 53bp)
TCAGGTACTTAGTGACTCTCAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	6	0.15	RNA PCR Primer, Index 11 (100% over 53bp)
TATCACAGCCAGCTTTGAGGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	6	0.15	RNA PCR Primer, Index 11 (100% over 53bp)
TGAGTATTACATCAGGTACTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCGCGTATGCCG	6	0.15	RNA PCR Primer, Index 11 (98% over 52bp)
TGACTAGATCCACACTCATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTCT	6	0.15	RNA PCR Primer, Index 11 (100% over 55bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCGCGTATGCCGTCTTCTGCTTGAAAGGGGGGGGG	5	0.125	RNA PCR Primer, Index 11 (98% over 63bp)
TGGAAGACTAGTGATTTTGTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	5	0.125	RNA PCR Primer, Index 11 (100% over 51bp)
TGAGTATTACATCAGGTACTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	5	0.125	RNA PCR Primer, Index 11 (100% over 53bp)
TATTGCACTTGAGACGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	5	0.125	RNA PCR Primer, Index 11 (100% over 53bp)
AAATATCAGCTGGTAATTCTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCG	5	0.125	RNA PCR Primer, Index 11 (100% over 52bp)
CGTACGGTTTAAACTTCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTT	5	0.125	RNA PCR Primer, Index 11 (100% over 56bp)
TGACTAGATCCACACTCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCGCGTATGCCGTC	5	0.125	RNA PCR Primer, Index 11 (98% over 54bp)
ATTGTACTTCATCAGGTGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCGCGTATGCCGT	5	0.125	RNA PCR Primer, Index 11 (98% over 53bp)
TAAGGAAATAGTAGCCGTGATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT	5	0.125	RNA PCR Primer, Index 11 (100% over 53bp)
TGACTAGATCCACACTCATTAAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCG	5	0.125	RNA PCR Primer, Index 11 (100% over 52bp)
TAGGAACTTCATACCGTGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCGCGTATGCCGT	5	0.125	RNA PCR Primer, Index 11 (98% over 53bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	8.925	0.0	0.0	0.0
2	0.0	9.0	0.0	0.0	0.0
3	0.0	9.075	0.0	0.0	0.0
4	0.0	9.15	0.0	0.0	0.0
5	0.0	9.175	0.0	0.0	0.0
6	0.0	9.3	0.0	0.0	0.0
7	0.0	9.3	0.0	0.0	0.0
8	0.0	9.375	0.0	0.0	0.0
9	0.0	9.4	0.0	0.0	0.0
10	0.0	9.4	0.0	0.0	0.0
11	0.0	9.4	0.0	0.0	0.0
12	0.0	9.425	0.0	0.0	0.0
13	0.0	9.475	0.0	0.0	0.0
14	0.0	9.625	0.0	0.0	0.0
15	0.0	9.75	0.0	0.0	0.0
16	0.0	10.15	0.0	0.0	0.0
17	0.0	10.7	0.0	0.0	0.0
18	0.0	11.575	0.0	0.0	0.0
19	0.0	12.8	0.0	0.0	0.0
20	0.0	15.25	0.0	0.0	0.0
21	0.0	18.325	0.0	0.0	0.0
22	0.0	27.875	0.0	0.0	0.0
23	0.0	46.05	0.0	0.0	0.0
24	0.0	56.05	0.0	0.0	0.0
25	0.0	66.85	0.0	0.0	0.0
26	0.0	78.65	0.0	0.0	0.0
27	0.0	87.45	0.0	0.0	0.0
28	0.0	93.6	0.0	0.0	0.0
29	0.0	95.85	0.0	0.0	0.0
30	0.0	96.175	0.0	0.0	0.0
31	0.0	96.325	0.0	0.0	0.0
32	0.0	96.4	0.0	0.0	0.0
33	0.0	96.5	0.0	0.0	0.0
34	0.0	96.625	0.0	0.0	0.0
35	0.0	96.65	0.0	0.0	0.0
36	0.0	96.675	0.0	0.0	0.0
37	0.0	96.675	0.0	0.0	0.0
38	0.0	96.75	0.0	0.0	0.0
39	0.0	96.875	0.0	0.0	0.0
40	0.0	96.875	0.0	0.0	0.0
41	0.0	96.95	0.0	0.0	0.0
42	0.0	96.975	0.0	0.0	0.0
43	0.0	96.975	0.0	0.0	0.0
44	0.0	97.05	0.0	0.0	0.0
45	0.0	97.125	0.0	0.0	0.0
46	0.0	97.3	0.0	0.0	0.0
47	0.0	97.375	0.0	0.0	0.0
48	0.0	97.5	0.0	0.0	0.0
49	0.0	97.65	0.0	0.0	0.0
50	0.0	97.7	0.0	0.0	0.0
51	0.0	97.7	0.0	0.0	0.0
52	0.0	97.7	0.0	0.0	0.0
53	0.0	97.7	0.0	0.0	0.0
54	0.0	97.7	0.0	0.0	0.0
55	0.0	97.7	0.0	0.0	0.0
56	0.0	97.7	0.0	0.0	0.0
57	0.0	97.7	0.0	0.0	0.0
58	0.0	97.725	0.0	0.0	0.0
59	0.0	97.75	0.0	0.0	0.0
60	0.0	97.75	0.0	0.0	0.0
61	0.0	97.775	0.0	0.0	0.0
62	0.0	97.8	0.0	0.0	0.0
63	0.0	97.825	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTCTA	15	0.0021872367	69.00001	3
GAAAAGG	15	0.0021872367	69.00001	63
AAAAGGG	15	0.0021872367	69.00001	64
CTTGAAA	30	8.704228E-8	69.00001	60
TTCTGCT	40	9.822543E-11	69.0	55
TCTGCTT	40	9.822543E-11	69.0	56
TGCTTGA	40	9.822543E-11	69.0	58
CTGCTTG	40	9.822543E-11	69.0	57
TTGAAAA	25	2.56746E-6	69.0	61
CTTCTGC	40	9.822543E-11	69.0	54
TGAAAAG	20	7.5213684E-5	69.0	62
GTCTTCT	45	2.7830538E-10	61.333332	52
GCTTGAA	45	2.7830538E-10	61.333332	59
TCTTCTG	45	2.7830538E-10	61.333332	53
CGTCTTC	45	2.7830538E-10	61.333332	51
AGGGGGG	30	7.578845E-6	57.500004	67
TATTGGA	25	2.2695167E-4	55.2	20
CCGTGAT	20	0.0068371096	51.75	15
GAAATAG	20	0.0068371096	51.75	5
AGTAGCC	20	0.0068371096	51.75	10
>>END_MODULE
Rejected 2585750 READS because READLEN < 1
Read 2585750 spots for SRR8447878.sra
Written 2585750 spots for SRR8447878.sra
Rejected 2585750 READS because READLEN < 1
Read 2585750 spots for SRR8447878.sra
Written 2585750 spots for SRR8447878.sra
Rejected 2585750 READS because READLEN < 1
Read 2585750 spots for SRR8447878.sra
Written 2585750 spots for SRR8447878.sra
Rejected 2585750 READS because READLEN < 1
Read 2585750 spots for SRR8447878.sra
Written 2585750 spots for SRR8447878.sra
Rejected 2585750 READS because READLEN < 1
Read 2585750 spots for SRR8447878.sra
Written 2585750 spots for SRR8447878.sra
Rejected 2585750 READS because READLEN < 1
Read 2585750 spots for SRR8447878.sra
Written 2585750 spots for SRR8447878.sra
Rejected 2585750 READS because READLEN < 1
Read 2585750 spots for SRR8447878.sra
Written 2585750 spots for SRR8447878.sra
Rejected 2585750 READS because READLEN < 1
Read 2585750 spots for SRR8447878.sra
Written 2585750 spots for SRR8447878.sra
Rejected 2585750 READS because READLEN < 1
Read 2585750 spots for SRR8447878.sra
Written 2585750 spots for SRR8447878.sra
Rejected 2585750 READS because READLEN < 1
Read 2585750 spots for SRR8447878.sra
Written 2585750 spots for SRR8447878.sra
Rejected 2585750 READS because READLEN < 1
Read 2585750 spots for SRR8447878.sra
Written 2585750 spots for SRR8447878.sra
Rejected 2585750 READS because READLEN < 1
Read 2585750 spots for SRR8447878.sra
Written 2585750 spots for SRR8447878.sra
Rejected 2585753 READS because READLEN < 1
Read 2585753 spots for SRR8447878.sra
Written 2585753 spots for SRR8447878.sra
Rejected 2585750 READS because READLEN < 1
Read 2585750 spots for SRR8447878.sra
Written 2585750 spots for SRR8447878.sra
Rejected 2585750 READS because READLEN < 1
Read 2585750 spots for SRR8447878.sra
Written 2585750 spots for SRR8447878.sra
Rejected 2585750 READS because READLEN < 1
Read 2585750 spots for SRR8447878.sra
Written 2585750 spots for SRR8447878.sra
SRR ids: ['SRR8447878.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_cq57_r_c
SRR8447878.sra spots: 41372003
blocks: [[1, 2585750], [2585751, 5171500], [5171501, 7757250], [7757251, 10343000], [10343001, 12928750], [12928751, 15514500], [15514501, 18100250], [18100251, 20686000], [20686001, 23271750], [23271751, 25857500], [25857501, 28443250], [28443251, 31029000], [31029001, 33614750], [33614751, 36200500], [36200501, 38786250], [38786251, 41372003]]
SRR8447878 file size 7816360
SRR8447878 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 16 -o SRR8447878 SRR8447878_1.fastq
Input file:	SRR8447878_1.fastq
trimmed:	SRR8447878-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	16
Sun Mar  1 22:48:34 2020 >> started

Sun Mar  1 22:51:42 2020 >> done (188.336s)
41372003 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
41372003 (100.00%) reads available; of these:
     221 ( 0.00%) trimmed reads available after processing
41371782 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 74	     221	  0.00%
 75	41371782	100.00%
41372003 reads passed initial QC


criterion=sequence-density
sequence-density=97.41
sequence-density-rank=1
fanout-score=49.94
fanout-score-rank=2
prefix-density=89.25
prefix-fanout=49.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=3
fanout-score=52.37
fanout-score-rank=1
prefix-density=1.15
prefix-fanout=1.0
sequence=CGACGATCTGTGATGTGACGTAGTGGAAC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 16 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAA -o SRR8447878 -
Input file:	STDIN
trimmed:	SRR8447878-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	16
Sun Mar  1 22:54:53 2020 >> started

Sun Mar  1 22:59:39 2020 >> done (286.074s)
40527677 reads processed; of these:
  948478 ( 2.34%) short reads filtered out after trimming by size control
 3577048 ( 8.83%) empty reads filtered out after trimming by size control
36002151 (88.83%) reads available; of these:
35638002 (98.99%) trimmed reads available after processing
  364149 ( 1.01%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  552572	  1.53%
 19	  975441	  2.71%
 20	 1395726	  3.88%
 21	 4110417	 11.42%
 22	 7463911	 20.73%
 23	 4163074	 11.56%
 24	 4027919	 11.19%
 25	 4689140	 13.02%
 26	 4031895	 11.20%
 27	 2545626	  7.07%
 28	  949026	  2.64%
 29	  158418	  0.44%
 30	   68949	  0.19%
 31	   39666	  0.11%
 32	   38094	  0.11%
 33	   34635	  0.10%
 34	   28624	  0.08%
 35	   22930	  0.06%
 36	   18980	  0.05%
 37	   17159	  0.05%
 38	   16924	  0.05%
 39	   14949	  0.04%
 40	   12814	  0.04%
 41	   13055	  0.04%
 42	   15171	  0.04%
 43	   21170	  0.06%
 44	   28210	  0.08%
 45	   27414	  0.08%
 46	   26846	  0.07%
 47	   25247	  0.07%
 48	   22652	  0.06%
 49	   18334	  0.05%
 50	   11579	  0.03%
 51	    5493	  0.02%
 52	    2223	  0.01%
 53	    1164	  0.00%
 54	    1018	  0.00%
 55	    1103	  0.00%
 56	    1468	  0.00%
 57	    1883	  0.01%
 58	    2489	  0.01%
 59	    2782	  0.01%
 60	    3272	  0.01%
 61	    3416	  0.01%
 62	    3720	  0.01%
 63	    3573	  0.01%
 64	    3311	  0.01%
 65	    3093	  0.01%
 66	    2574	  0.01%
 67	    2483	  0.01%
 68	    2115	  0.01%
 69	    1245	  0.00%
 70	     848	  0.00%
 71	     600	  0.00%
 72	    1562	  0.00%
 73	       0	  0.00%
 74	       3	  0.00%
 75	  364146	  1.01%


criterion=sequence-density
sequence-density=1.43
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=33
prefix-density=0.04
prefix-fanout=1.0
sequence=TATCACAGCCATTTTGACGAGTTAAA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=31
fanout-score=64.01
fanout-score-rank=1
prefix-density=1.02
prefix-fanout=1.0
sequence=CCGACGATCATTGTACTTCATCAGGTGCTCTGGT
                                 Started job on |	Mar 01 23:02:24
                             Started mapping on |	Mar 01 23:02:25
                                    Finished on |	Mar 01 23:13:49
       Mapping speed, Million of reads per hour |	193.93

                          Number of input reads |	36846477
                      Average input read length |	25
                                    UNIQUE READS:
                   Uniquely mapped reads number |	14667234
                        Uniquely mapped reads % |	39.81%
                          Average mapped length |	22.32
                       Number of splices: Total |	117790
            Number of splices: Annotated (sjdb) |	12764
                       Number of splices: GT/AG |	114066
                       Number of splices: GC/AG |	2401
                       Number of splices: AT/AC |	8
               Number of splices: Non-canonical |	1315
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.20
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.01
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	7652366
             % of reads mapped to multiple loci |	20.77%
        Number of reads mapped to too many loci |	8781000
             % of reads mapped to too many loci |	23.83%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.57%
                     % of reads unmapped: other |	10.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	14526877	14526877	14526877
N_multimapping	7652366	7652366	7652366
N_noFeature	2977126	3103749	14487589
N_ambiguous	4488525	4432459	5900
UnstrandedReadsAssigned:7201583 PositiveStrandReadsAssigned:7131026 NegativeStrandReadsAssigned:173745
Dataset is classified positive stranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR8447878 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 30,651
[index] number of k-mers: 33,029,304
[index] number of equivalence classes: 113,299
[quant] running in single-end mode
[quant] will process file 1: SRR8447878-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 36,846,477 reads, 2,817,362 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,164 rounds

  30652 SRR8447878.ke.tsv
  17559 SRR8447878.se.tsv
  48211 total
==> SRR8447878.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
FBtr0082757	1844	1745	2	0.344803
FBtr0080129	599	500	0	0
FBtr0336850	943	844	2	0.712892
FBtr0113346	1241	1142	8	2.10746
FBtr0082908	740	641	0	0
FBtr0078480	1061	962	0	0
FBtr0334111	1310	1211	16	3.97477
FBtr0084333	1249	1150	0	0
FBtr0335007	714	615	0	0

==> SRR8447878.se.tsv <==
FBgn0085804	6
FBgn0267431	84
FBgn0039987	3
FBgn0058182	14
FBgn0267430	41
FBgn0266747	5
FBgn0086917	22
FBgn0010247	69
FBgn0086378	210
FBgn0263977	51
SRR8447878 completed mapping pipeline successfully
