Starting /dee2/code/volunteer_pipeline.sh SRR23332655
    current disk space = 1435013320
    free memory = 57321736 
SRR23332655 SRAfilesize
a8581cb0299064a5b9f35639e7e49330  SRR23332655.sra
SRR23332655.sra file validated
SRR23332655 is paired end
SRR23332655 is conventional basespace
SRR23332655 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332655_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.4795	37.0	37.0	37.0	37.0	37.0
2	36.5075	37.0	37.0	37.0	37.0	37.0
3	36.512	37.0	37.0	37.0	37.0	37.0
4	36.537	37.0	37.0	37.0	37.0	37.0
5	36.336	37.0	37.0	37.0	37.0	37.0
6	36.489	37.0	37.0	37.0	37.0	37.0
7	36.424	37.0	37.0	37.0	37.0	37.0
8	36.4775	37.0	37.0	37.0	37.0	37.0
9	36.541	37.0	37.0	37.0	37.0	37.0
10	36.57	37.0	37.0	37.0	37.0	37.0
11	36.552	37.0	37.0	37.0	37.0	37.0
12	36.4765	37.0	37.0	37.0	37.0	37.0
13	36.5055	37.0	37.0	37.0	37.0	37.0
14	36.465	37.0	37.0	37.0	37.0	37.0
15	36.515	37.0	37.0	37.0	37.0	37.0
16	36.5255	37.0	37.0	37.0	37.0	37.0
17	36.451	37.0	37.0	37.0	37.0	37.0
18	36.3735	37.0	37.0	37.0	37.0	37.0
19	36.408	37.0	37.0	37.0	37.0	37.0
20	36.4635	37.0	37.0	37.0	37.0	37.0
21	36.468	37.0	37.0	37.0	37.0	37.0
22	36.4555	37.0	37.0	37.0	37.0	37.0
23	36.3815	37.0	37.0	37.0	37.0	37.0
24	36.406	37.0	37.0	37.0	37.0	37.0
25	36.526	37.0	37.0	37.0	37.0	37.0
26	36.3495	37.0	37.0	37.0	37.0	37.0
27	36.4365	37.0	37.0	37.0	37.0	37.0
28	36.478	37.0	37.0	37.0	37.0	37.0
29	36.3585	37.0	37.0	37.0	37.0	37.0
30	36.47	37.0	37.0	37.0	37.0	37.0
31	36.562	37.0	37.0	37.0	37.0	37.0
32	36.436	37.0	37.0	37.0	37.0	37.0
33	36.476	37.0	37.0	37.0	37.0	37.0
34	36.3435	37.0	37.0	37.0	37.0	37.0
35	36.3185	37.0	37.0	37.0	37.0	37.0
36	36.424	37.0	37.0	37.0	37.0	37.0
37	36.381	37.0	37.0	37.0	37.0	37.0
38	36.4405	37.0	37.0	37.0	37.0	37.0
39	36.3255	37.0	37.0	37.0	37.0	37.0
40	36.3615	37.0	37.0	37.0	37.0	37.0
41	36.448	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	2.0
24	0.0
25	1.0
26	3.0
27	5.0
28	7.0
29	27.0
30	25.0
31	25.0
32	52.0
33	77.0
34	78.0
35	191.0
36	1213.0
37	2294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.825	19.775000000000002	46.575	8.825
2	32.675	17.525	27.650000000000002	22.15
3	33.15	20.775	24.349999999999998	21.725
4	19.2	23.925	35.85	21.025
5	34.849999999999994	22.075	21.175	21.9
6	35.949999999999996	22.425	18.475	23.150000000000002
7	21.625	33.45	19.400000000000002	25.525
8	37.625	17.25	20.3	24.825
9	24.474999999999998	14.674999999999999	21.0	39.85
10	25.2	16.025	33.650000000000006	25.124999999999996
11	38.5	17.1	20.625	23.775
12	38.9	18.099999999999998	20.25	22.75
13	25.2	18.55	20.45	35.8
14	37.0	21.175	20.150000000000002	21.675
15	38.074999999999996	21.725	19.375	20.825
16	40.525	21.125	19.525000000000002	18.825
17	24.4	34.425	20.95	20.225
18	38.3	18.75	21.95	21.0
19	24.7	19.35	36.0	19.950000000000003
20	25.05	20.05	33.775	21.125
21	39.300000000000004	21.7	18.4	20.599999999999998
22	24.0	33.975	19.625	22.400000000000002
23	23.825	33.475	19.400000000000002	23.3
24	22.35	18.85	20.9	37.9
25	25.525	17.4	20.65	36.425000000000004
26	24.975	17.825	22.55	34.65
27	38.3	19.2	20.825	21.675
28	22.725	16.625	23.425	37.225
29	37.625	18.5	24.0	19.875
30	21.475	18.475	38.9	21.15
31	22.925	19.625	23.325000000000003	34.125
32	21.0	21.2	22.525000000000002	35.275
33	34.8	24.2	20.875	20.125
34	21.575	37.175000000000004	20.825	20.424999999999997
35	22.0	35.775	21.575	20.65
36	21.925	35.65	20.8	21.625
37	23.325000000000003	21.15	33.324999999999996	22.2
38	24.175	20.175	34.175	21.475
39	22.025	22.275	19.25	36.449999999999996
40	20.1	22.55	20.075000000000003	37.275000000000006
41	20.375	22.075	35.875	21.675
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	1.0
16	1.0
17	1.0
18	0.5
19	0.0
20	0.0
21	0.5
22	1.0
23	2.5
24	4.0
25	4.0
26	5.0
27	6.0
28	11.0
29	16.0
30	16.0
31	16.0
32	16.0
33	23.5
34	31.0
35	32.5
36	34.0
37	34.0
38	62.0
39	90.0
40	126.0
41	162.0
42	162.0
43	182.5
44	203.0
45	283.5
46	364.0
47	364.0
48	370.5
49	377.0
50	404.0
51	431.0
52	466.5
53	502.0
54	502.0
55	431.0
56	360.0
57	337.0
58	314.0
59	314.0
60	589.0
61	864.0
62	496.0
63	128.0
64	128.0
65	86.5
66	45.0
67	35.5
68	26.0
69	26.0
70	19.5
71	13.0
72	9.5
73	6.0
74	3.5
75	1.0
76	1.0
77	1.5
78	2.0
79	1.0
80	0.0
81	0.0
82	0.5
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.8
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.50836120401338	46.35
2	15.259197324414716	18.25
3	3.7625418060200673	6.75
4	1.6304347826086956	3.9
5	0.5852842809364548	1.7500000000000002
6	0.2926421404682274	1.05
7	0.1254180602006689	0.525
8	0.1254180602006689	0.6
9	0.2508361204013378	1.35
>10	0.4180602006688963	5.075
>50	0.0	0.0
>100	0.0	0.0
>500	0.04180602006688963	14.399999999999999
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCT	576	14.399999999999999	No Hit
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTAC	37	0.9249999999999999	No Hit
GGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTA	35	0.8750000000000001	No Hit
GCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACA	35	0.8750000000000001	No Hit
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCT	23	0.575	TruSeq Adapter, Index 8 (97% over 37bp)
GGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAAC	18	0.44999999999999996	No Hit
AAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAA	12	0.3	No Hit
AAAAAACGCGCCCGAAGGCGCGTTGGCGATACACTCAATAT	12	0.3	No Hit
TGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	11	0.27499999999999997	No Hit
GGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAACC	10	0.25	No Hit
ACAAACGGCACGACACAGCTGTCGTACCGTTATTGCTTATT	10	0.25	No Hit
GCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACTAC	9	0.22499999999999998	No Hit
ACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACTGGG	9	0.22499999999999998	No Hit
TGCAGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATC	9	0.22499999999999998	No Hit
GTCGCCCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAAT	9	0.22499999999999998	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCC	9	0.22499999999999998	No Hit
AAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTT	9	0.22499999999999998	No Hit
GGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACAT	8	0.2	No Hit
TACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACTGG	8	0.2	No Hit
TTCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTCCT	8	0.2	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACGAGAGGACCGGGA	7	0.17500000000000002	No Hit
ATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCA	7	0.17500000000000002	No Hit
TGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACA	7	0.17500000000000002	No Hit
TTCCGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTT	6	0.15	No Hit
GTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCA	6	0.15	No Hit
GGATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGG	6	0.15	No Hit
TCATGGAGTCGAGTTGCTAGTAATCGTGGATCAGAATGCCA	6	0.15	No Hit
GTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTC	6	0.15	No Hit
TCGTACTAGGAGTGGACGCATCACTGGTGTTCGGGTTGTCA	6	0.15	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCC	6	0.15	No Hit
TAACAAACTAGCCTGATTAAGTTTTAACGCTTCAACCCCAG	5	0.125	No Hit
TCCGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTA	5	0.125	No Hit
GTCTCACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGAT	5	0.125	No Hit
AGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAA	5	0.125	No Hit
GCAACCCACTTTGTGATTCATGACTGGGGTGAAGTCGTAAC	5	0.125	No Hit
ACTAACAAACTAGCCTGATTAAGTTTTAACGCTTCAACCCC	5	0.125	No Hit
TTTATCCGTTGAGCTAACCGGTACTAATGAACCGTGTCTCC	5	0.125	No Hit
AGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGT	5	0.125	No Hit
TTTATGAGGTCGTAGTCCGGATTGGAGTCTGCAACTCGACT	5	0.125	No Hit
TCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACA	5	0.125	No Hit
GTTTTAACGCTTCAACCCCAGGCAGGGCTTCCACGCGATCT	5	0.125	No Hit
ATCAACGGCTCCTGAATCAGGAGCCGTCTTATTACTGCATA	5	0.125	No Hit
AAGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGA	5	0.125	No Hit
GGCGGTGTGTACAAGGTAACCGTAGGGGAACCTGCGGTTGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.6	0.0	0.0	0.0	0.0
5	0.65	0.0	0.0	0.0	0.0
6	0.675	0.0	0.0	0.0	0.0
7	0.7	0.0	0.0	0.0	0.0
8	0.7	0.0	0.0	0.0	0.0
9	0.7	0.0	0.0	0.0	0.0
10	0.725	0.0	0.0	0.0	0.0
11	0.725	0.0	0.0	0.0	0.0
12	0.725	0.0	0.0	0.0	0.0
13	0.75	0.0	0.0	0.0	0.0
14	0.75	0.0	0.0	0.0	0.0
15	0.775	0.0	0.0	0.0	0.0
16	0.775	0.0	0.0	0.0	0.0
17	0.8	0.0	0.0	0.0	0.0
18	0.875	0.0	0.0	0.0	0.0
19	0.9	0.0	0.0	0.0	0.0
20	0.9	0.0	0.0	0.0	0.0
21	0.975	0.0	0.0	0.0	0.0
22	1.0	0.0	0.0	0.0	0.0
23	1.125	0.0	0.0	0.0	0.0
24	1.15	0.0	0.0	0.0	0.0
25	1.3	0.0	0.0	0.0	0.0
26	1.45	0.0	0.0	0.0	0.0
27	1.55	0.0	0.0	0.0	0.0
28	1.625	0.0	0.0	0.0	0.0
29	1.725	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGAGCT	60	0.0	35.000004	4
GGTGGAG	60	0.0	35.000004	2
TGGTGGA	60	0.0	35.000004	1
GTGGAGC	60	0.0	35.000004	3
GCGGGAG	70	0.0	32.500004	12
GTTGAAC	70	0.0	32.500004	18
GCTGGCG	70	0.0	32.500004	8
ACCCGCG	70	0.0	32.500004	23
GGGAGTT	70	0.0	32.500004	14
GAGTTGA	70	0.0	32.500004	16
CTGGCGG	70	0.0	32.500004	9
CCCGCGT	70	0.0	32.500004	24
GAACCCG	70	0.0	32.500004	21
CGGGAGT	70	0.0	32.500004	13
GCGTCCG	70	0.0	32.500004	27
AGTTGAA	70	0.0	32.500004	17
TGGCGGG	70	0.0	32.500004	10
TGAACCC	70	0.0	32.500004	20
GGAGTTG	70	0.0	32.500004	15
CGCGTCC	70	0.0	32.500004	26
>>END_MODULE
SRR23332655 read2 length is 27-50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332655_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	27-50
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	19.883	11.0	11.0	37.0	11.0	37.0
2	23.382	25.0	11.0	37.0	11.0	37.0
3	25.623	25.0	11.0	37.0	11.0	37.0
4	35.7065	37.0	37.0	37.0	37.0	37.0
5	35.984	37.0	37.0	37.0	37.0	37.0
6	35.9225	37.0	37.0	37.0	37.0	37.0
7	35.789	37.0	37.0	37.0	37.0	37.0
8	35.888	37.0	37.0	37.0	37.0	37.0
9	35.44	37.0	37.0	37.0	37.0	37.0
10	35.868	37.0	37.0	37.0	37.0	37.0
11	35.8515	37.0	37.0	37.0	37.0	37.0
12	35.8835	37.0	37.0	37.0	37.0	37.0
13	35.9065	37.0	37.0	37.0	37.0	37.0
14	36.0365	37.0	37.0	37.0	37.0	37.0
15	35.8505	37.0	37.0	37.0	37.0	37.0
16	36.038	37.0	37.0	37.0	37.0	37.0
17	35.8895	37.0	37.0	37.0	37.0	37.0
18	35.997	37.0	37.0	37.0	37.0	37.0
19	36.1125	37.0	37.0	37.0	37.0	37.0
20	36.0855	37.0	37.0	37.0	37.0	37.0
21	35.90225	37.0	37.0	37.0	37.0	37.0
22	36.096	37.0	37.0	37.0	37.0	37.0
23	35.9875	37.0	37.0	37.0	37.0	37.0
24	36.037	37.0	37.0	37.0	37.0	37.0
25	36.0445	37.0	37.0	37.0	37.0	37.0
26	36.16	37.0	37.0	37.0	37.0	37.0
27	36.00275	37.0	37.0	37.0	37.0	37.0
28	35.968992248062015	37.0	37.0	37.0	37.0	37.0
29	35.96574143535884	37.0	37.0	37.0	37.0	37.0
30	35.98149537384346	37.0	37.0	37.0	37.0	37.0
31	35.99674918729682	37.0	37.0	37.0	37.0	37.0
32	35.99199799949987	37.0	37.0	37.0	37.0	37.0
33	35.771942985746435	37.0	37.0	37.0	37.0	37.0
34	36.00475118779695	37.0	37.0	37.0	37.0	37.0
35	36.040260065016255	37.0	37.0	37.0	37.0	37.0
36	36.1328164082041	37.0	37.0	37.0	37.0	37.0
37	35.99049524762381	37.0	37.0	37.0	37.0	37.0
38	35.894447223611806	37.0	37.0	37.0	37.0	37.0
39	36.04152076038019	37.0	37.0	37.0	37.0	37.0
40	35.980990495247624	37.0	37.0	37.0	37.0	37.0
41	35.70885442721361	37.0	37.0	37.0	37.0	37.0
42	35.89116837628221	37.0	37.0	37.0	37.0	37.0
43	35.855141356017015	37.0	37.0	37.0	37.0	37.0
44	35.989914271306105	37.0	37.0	37.0	37.0	37.0
45	35.759791122715406	37.0	37.0	37.0	37.0	37.0
46	35.872564507635595	37.0	37.0	37.0	37.0	37.0
47	35.975713904456896	37.0	37.0	37.0	37.0	37.0
48	35.96322336398053	37.0	37.0	37.0	37.0	37.0
49	35.912566686425606	37.0	37.0	37.0	37.0	37.0
50	35.916819347746426	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	1.0
18	3.0
19	5.0
20	3.0
21	6.0
22	9.0
23	7.0
24	15.0
25	12.0
26	13.0
27	20.0
28	21.0
29	28.0
30	43.0
31	60.0
32	92.0
33	166.0
34	429.0
35	1695.0
36	1210.0
37	160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	38.4	22.45	39.15
2	30.5	32.375	14.025000000000002	23.1
3	30.475	41.05	10.25	18.224999999999998
4	81.35	6.275	4.625	7.75
5	67.45	15.049999999999999	9.8	7.7
6	59.22500000000001	20.474999999999998	7.925	12.375
7	37.85	39.15	10.15	12.85
8	42.6	20.9	13.25	23.25
9	21.425	38.9	26.55	13.125
10	42.3	22.650000000000002	17.075000000000003	17.974999999999998
11	28.849999999999998	39.675	16.925	14.549999999999999
12	22.25	28.325	26.8	22.625
13	37.5	14.124999999999998	28.849999999999998	19.525000000000002
14	34.275	15.6	26.375	23.75
15	28.275	17.05	43.65	11.025
16	47.199999999999996	21.4	20.9	10.5
17	28.675	33.375	17.525	20.424999999999997
18	16.05	39.15	28.475	16.325
19	19.325	19.55	34.949999999999996	26.174999999999997
20	15.75	23.7	31.874999999999996	28.675
21	20.530132533133283	41.98549637409352	12.603150787696924	24.88122030507627
22	39.225	30.349999999999998	12.775	17.65
23	21.55	31.0	18.75	28.7
24	24.7	22.825	12.325	40.150000000000006
25	40.849999999999994	16.775000000000002	12.725	29.65
26	33.95	22.275	12.55	31.225
27	38.50962740685171	27.506876719179797	19.554888722180543	14.428607151787947
28	34.092046023011505	26.21310655327664	26.538269134567283	13.156578289144571
29	24.756189047261813	14.95373843460865	46.28657164291073	14.003500875218805
30	18.3591795897949	16.45822911455728	52.10105052526263	13.081540770385192
31	17.75443860965241	21.85546386596649	40.11002750687672	20.280070017504375
32	33.291645822911455	13.956978489244623	20.060030015007506	32.69134567283642
33	22.311155577788895	13.356678339169584	15.232616308154077	49.09954977488744
34	27.045283962972228	18.363772829622217	21.31598699024268	33.274956217162874
35	14.128532133033259	34.133533383345835	21.555388847211805	30.182545636409102
36	12.859644733550162	40.70552914686015	29.797348011008257	16.637478108581437
37	17.208604302151077	44.772386193096544	13.256628314157078	24.7623811905953
38	21.310655327663834	19.984992496248125	10.105052526263131	48.599299649824914
39	37.1935967983992	18.10905452726363	10.705352676338169	33.991995997999
40	23.411705852926463	20.68534267133567	18.409204602301152	37.49374687343672
41	17.10855427713857	40.54527263631816	26.988494247123562	15.35767883941971
42	21.841381035776834	29.97247935951964	28.396297222917187	19.78984238178634
43	33.19989992494371	23.942957217913435	14.260695521641232	28.596447335501622
44	38.451840645486634	14.548663640948059	18.885526979324258	28.113968734241052
45	19.68668407310705	15.535248041775457	33.81201044386423	30.966057441253263
46	29.383886255924168	18.193786203264875	32.59610321221695	19.826224328593998
47	30.717907659460902	15.719242060314919	25.113424072591407	28.449426207632772
48	28.339643050297457	25.77068685776095	25.283937263385614	20.605732828555976
49	30.418025496590573	12.985472872813519	41.65431366735844	14.942187963237474
50	32.68596555514841	13.411506046170759	27.299377061194573	26.60315133748626
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.5
6	2.0
7	1.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	1.5
18	2.0
19	1.5
20	1.0
21	1.0
22	1.0
23	1.0
24	1.0
25	3.0
26	5.0
27	5.5
28	7.5
29	10.0
30	11.5
31	17.5
32	24.0
33	35.5
34	50.0
35	55.5
36	58.5
37	61.0
38	66.0
39	71.0
40	70.5
41	81.5
42	97.5
43	102.0
44	101.0
45	109.0
46	125.5
47	149.0
48	170.5
49	181.5
50	194.0
51	330.5
52	480.0
53	640.0
54	812.5
55	822.5
56	778.5
57	676.0
58	548.5
59	387.5
60	247.0
61	154.5
62	83.0
63	62.0
64	45.0
65	37.0
66	26.5
67	22.0
68	20.0
69	13.0
70	7.5
71	6.0
72	4.5
73	2.5
74	0.5
75	0.5
76	1.0
77	1.5
78	2.0
79	2.5
80	3.5
81	4.0
82	4.0
83	2.5
84	1.0
85	1.0
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.025
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.025
28	0.025006251562890724
29	0.0
30	0.025006251562890724
31	0.0
32	0.025006251562890724
33	0.025006251562890724
34	0.05001250312578145
35	0.0
36	0.02501250625312656
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.02963841138114997
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
27	1.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	1.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	1.0
42	0.0
43	31.0
44	136.0
45	32.0
46	51.0
47	49.0
48	324.0
49	645.0
50	2729.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.599999999999994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.57718120805369	51.6
2	5.075503355704698	6.05
3	1.8456375838926176	3.3000000000000003
4	1.2583892617449663	3.0
5	1.0906040268456376	3.25
6	0.587248322147651	2.1
7	0.6291946308724832	2.625
8	0.41946308724832215	2.0
9	0.33557046979865773	1.7999999999999998
>10	2.097315436241611	20.4
>50	0.08389261744966443	3.875
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	84	2.1	No Hit
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	71	1.775	No Hit
AAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	45	1.125	No Hit
CCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	38	0.95	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	35	0.8750000000000001	No Hit
CCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	30	0.75	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	28	0.7000000000000001	No Hit
AAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	26	0.65	No Hit
CGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	25	0.625	No Hit
CAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	23	0.575	No Hit
CGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	20	0.5	No Hit
TAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	20	0.5	No Hit
CCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	19	0.475	No Hit
ATAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	19	0.475	No Hit
CAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	18	0.44999999999999996	No Hit
CACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	17	0.42500000000000004	No Hit
CGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	17	0.42500000000000004	No Hit
ACAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	17	0.42500000000000004	No Hit
CAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	17	0.42500000000000004	No Hit
CCAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	16	0.4	No Hit
AACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
ATAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	16	0.4	No Hit
CCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	16	0.4	No Hit
CGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
CTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
CGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	15	0.375	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	14	0.35000000000000003	No Hit
CCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	14	0.35000000000000003	No Hit
AAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
CCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
CGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
ACAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
ACCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
AGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
TGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	12	0.3	No Hit
AAAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	11	0.27499999999999997	No Hit
CCCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	11	0.27499999999999997	No Hit
CGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
CGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
AGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	11	0.27499999999999997	No Hit
CTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
TGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
CGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
CGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
TACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	10	0.25	No Hit
ACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
TGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
CGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
AGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
CTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTA	9	0.22499999999999998	No Hit
CGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
ATAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
CGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	9	0.22499999999999998	No Hit
ACCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
TAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
ACAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
CGCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
CGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	8	0.2	No Hit
TGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
ACCCTCGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGAG	8	0.2	Illumina Single End PCR Primer 1 (97% over 34bp)
TGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
TAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
ATAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
TCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
TTAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
AAAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAT	7	0.17500000000000002	No Hit
TTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CTCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AAAGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTA	7	0.17500000000000002	No Hit
ACCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CCCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
CCGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
AGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CCCGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	6	0.15	No Hit
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTA	6	0.15	No Hit
ACAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
ATTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
AGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	6	0.15	No Hit
AATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AAAGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCT	5	0.125	No Hit
AGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TTGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
ACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CCGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CCCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAT	5	0.125	No Hit
AGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CGTGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
ATTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
ATAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	5	0.125	No Hit
CTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CACGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CCGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TTTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.225	0.0	0.0	0.0	0.0
11	0.3	0.0	0.0	0.0	0.0
12	0.475	0.0	0.0	0.0	0.0
13	0.7	0.0	0.0	0.0	0.0
14	0.7	0.0	0.0	0.0	0.0
15	0.7	0.0	0.0	0.0	0.0
16	0.725	0.0	0.0	0.0	0.0
17	0.75	0.0	0.0	0.0	0.0
18	0.75	0.0	0.0	0.0	0.0
19	0.75	0.0	0.0	0.0	0.0
20	0.75	0.0	0.0	0.0	0.0
21	0.75	0.0	0.0	0.0	0.0
22	0.775	0.0	0.0	0.0	0.0
23	0.825	0.0	0.0	0.0	0.0
24	0.85	0.0	0.0	0.0	0.0
25	0.9	0.0	0.0	0.0	0.0
26	0.95	0.0	0.0	0.0	0.0
27	1.0	0.0	0.0	0.0	0.0
28	1.075	0.0	0.0	0.0	0.0
29	1.1	0.0	0.0	0.0	0.0
30	1.1	0.0	0.0	0.0	0.0
31	1.225	0.0	0.0	0.0	0.0
32	1.3	0.0	0.0	0.0	0.0
33	1.375	0.0	0.0	0.0	0.0
34	1.45	0.0	0.0	0.0	0.0
35	1.575	0.0	0.0	0.0	0.0
36	1.6	0.0	0.0	0.0	0.0
37	1.675	0.0	0.0	0.0	0.0
38	1.8	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGCGCT	15	0.0022840165	67.5098	44
GTGCATG	60	0.0	56.25817	44
GTAGTGC	20	0.0071402765	50.632355	44
CCTTGTT	55	1.5315891E-9	49.09804	44
AAGGGAG	30	2.2727982E-6	43.037502	2
AGGGAGA	45	3.4015102E-10	43.037502	3
CGGGAGA	20	7.7526853E-4	43.037502	3
AAAGGGA	25	4.211841E-5	43.0375	1
GGTGCAT	65	1.8189894E-11	42.039074	43
AGGTGCA	60	2.7284841E-11	40.9881	42
AAGGTGC	60	3.274181E-11	40.410797	41
CAAGGTG	60	5.0931703E-11	38.772522	40
CCAAGGT	60	5.0931703E-11	38.772522	39
GGGAGAG	45	1.8319042E-8	38.255558	4
GAGAGGA	45	1.8319042E-8	38.255558	6
AGGGGCT	35	6.563472E-6	36.889286	3
ACCTTGT	60	2.484012E-8	36.433865	43
TCGACGG	60	1.2732926E-10	35.864586	20
GAAACCC	60	1.2732926E-10	35.864586	34
TCTGGAT	60	1.2732926E-10	35.864586	13
>>END_MODULE
Read 553250 spots for SRR23332655.sra
Written 553250 spots for SRR23332655.sra
Read 553254 spots for SRR23332655.sra
Written 553254 spots for SRR23332655.sra
Read 553250 spots for SRR23332655.sra
Written 553250 spots for SRR23332655.sra
Read 553250 spots for SRR23332655.sra
Written 553250 spots for SRR23332655.sra
Read 553250 spots for SRR23332655.sra
Written 553250 spots for SRR23332655.sra
Read 553250 spots for SRR23332655.sra
Written 553250 spots for SRR23332655.sra
Read 553250 spots for SRR23332655.sra
Written 553250 spots for SRR23332655.sra
Read 553250 spots for SRR23332655.sra
Written 553250 spots for SRR23332655.sra
SRR ids: ['SRR23332655.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_sgxqljvz
SRR23332655.sra spots: 4426004
blocks: [[1, 553250], [553251, 1106500], [1106501, 1659750], [1659751, 2213000], [2213001, 2766250], [2766251, 3319500], [3319501, 3872750], [3872751, 4426004]]
SRR23332655 file size 538116
SRR23332655 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR23332655 SRR23332655_1.fastq SRR23332655_2.fastq
Input file:	SRR23332655_1.fastq
Paired file:	SRR23332655_2.fastq
trimmed:	SRR23332655-trimmed-pair1.fastq, SRR23332655-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 05:10:04 2026 >> started

Wed Mar 25 05:10:07 2026 >> done (3.135s)
4426004 read pairs processed; of these:
    912 ( 0.02%) short read pairs filtered out after trimming by size control
     23 ( 0.00%) empty read pairs filtered out after trimming by size control
4425069 (99.98%) read pairs available; of these:
  33487 ( 0.76%) trimmed read pairs available after processing
4391582 (99.24%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    342	  0.01%
 19	    494	  0.01%
 20	    634	  0.01%
 21	    587	  0.01%
 22	    661	  0.01%
 23	    673	  0.02%
 24	    787	  0.02%
 25	   1055	  0.02%
 26	    904	  0.02%
 27	    879	  0.02%
 28	    830	  0.02%
 29	   2325	  0.05%
 30	    971	  0.02%
 31	   1210	  0.03%
 32	   1193	  0.03%
 33	   1471	  0.03%
 34	   1947	  0.04%
 35	   1867	  0.04%
 36	   1586	  0.04%
 37	   1667	  0.04%
 38	   2091	  0.05%
 39	   2320	  0.05%
 40	   4838	  0.11%
 41	   5779	  0.13%
 42	 210164	  4.75%
 43	 108292	  2.45%
 44	 482757	 10.91%
 45	3586745	 81.06%
4425069 reads passed initial QC


criterion=sequence-density
sequence-density=1.37
sequence-density-rank=1
fanout-score=1.92
fanout-score-rank=20
prefix-density=1.39
prefix-fanout=1.9
sequence=GTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAAT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=32
fanout-score=30.34
fanout-score-rank=1
prefix-density=0.30
prefix-fanout=1.2
sequence=TTTTTTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACAGAATCAGGCCATTACTACCCTGTCCGCCATGGCTTCTCGCCAGCCTCCCAGCCATTGTGACCTTTGATTCAGCGTCTGATAGGGACACATTTCTTTTGAGCGTCCGGCGATGCCGGCCTGATAACCACGTTGATGTGCCCGTTCCAGGCGATCTCGTTTTTGTCTCTTCATGCCTCGTTTCCCTCATACTGGTTTCT


criterion=sequence-density
sequence-density=15.75
sequence-density-rank=1
fanout-score=20.33
fanout-score-rank=6
prefix-density=12.84
prefix-fanout=20.3
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACC


criterion=fanout-score
sequence-density=0.42
sequence-density-rank=12
fanout-score=258.61
fanout-score-rank=1
prefix-density=9.28
prefix-fanout=11.6
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTACCCTCGGC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAAT -y GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACC -o SRR23332655 SRR23332655_1.fastq SRR23332655_2.fastq
Input file:	SRR23332655_1.fastq
Paired file:	SRR23332655_2.fastq
trimmed:	SRR23332655-trimmed-pair1.fastq, SRR23332655-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAA
-- paired 3' end adapter sequence (-y):	GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 05:10:16 2026 >> started

Wed Mar 25 05:10:19 2026 >> done (3.033s)
3441720 read pairs processed; of these:
  36715 ( 1.07%) short read pairs filtered out after trimming by size control
   1329 ( 0.04%) empty read pairs filtered out after trimming by size control
3403676 (98.89%) read pairs available; of these:
   1375 ( 0.04%) trimmed read pairs available after processing
3402301 (99.96%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    274	  0.01%
 19	    375	  0.01%
 20	    478	  0.01%
 21	    469	  0.01%
 22	    516	  0.02%
 23	    529	  0.02%
 24	    636	  0.02%
 25	    822	  0.02%
 26	    698	  0.02%
 27	    672	  0.02%
 28	    649	  0.02%
 29	   1821	  0.05%
 30	    788	  0.02%
 31	    907	  0.03%
 32	    938	  0.03%
 33	   1156	  0.03%
 34	   1543	  0.05%
 35	   1462	  0.04%
 36	   1248	  0.04%
 37	   1305	  0.04%
 38	   1622	  0.05%
 39	   1797	  0.05%
 40	   3811	  0.11%
 41	   4505	  0.13%
 42	 163227	  4.80%
 43	  84153	  2.47%
 44	 364016	 10.69%
 45	2763259	 81.18%


criterion=sequence-density
sequence-density=1.37
sequence-density-rank=1
fanout-score=1.91
fanout-score-rank=23
prefix-density=1.22
prefix-fanout=1.9
sequence=GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=32
fanout-score=28.01
fanout-score-rank=1
prefix-density=0.28
prefix-fanout=1.2
sequence=TTTTTTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACAGAATCAGGCCATTACTACCCTGTCCGCCATGGCTTCTCGCCAGCCTCCCAGCCATTGTGACCTTTGATTCAGCGTCTGATAGGGACACATTTCTTTTGAGCGTCCGGCGATGCCGGCCTGATAACCACGTTGATGTGCCCGTTCCAGGCGATCTCGTTTTTGTCTCTTCATGCCTCGTTTCCCTCATACTGGTTTCT


criterion=sequence-density
sequence-density=14.90
sequence-density-rank=1
fanout-score=19.67
fanout-score-rank=6
prefix-density=12.52
prefix-fanout=19.7
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACC


criterion=fanout-score
sequence-density=0.42
sequence-density-rank=12
fanout-score=257.78
fanout-score-rank=1
prefix-density=9.40
prefix-fanout=11.5
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTACCCTCGGC
SRR23332655 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 25 05:10:37
                             Started mapping on |	Mar 25 05:10:37
                                    Finished on |	Mar 25 05:13:27
       Mapping speed, Million of reads per hour |	92.90

                          Number of input reads |	4387025
                      Average input read length |	90
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2393378
                        Uniquely mapped reads % |	54.56%
                          Average mapped length |	87.71
                       Number of splices: Total |	137119
            Number of splices: Annotated (sjdb) |	135
                       Number of splices: GT/AG |	127179
                       Number of splices: GC/AG |	2713
                       Number of splices: AT/AC |	248
               Number of splices: Non-canonical |	6979
                      Mismatch rate per base, % |	0.28%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.34
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.03
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	929770
             % of reads mapped to multiple loci |	21.19%
        Number of reads mapped to too many loci |	756924
             % of reads mapped to too many loci |	17.25%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.69%
                     % of reads unmapped: other |	0.31%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1063879	1063879	1063879
N_multimapping	929770	929770	929770
N_noFeature	79808	2175300	166400
N_ambiguous	223524	34912	64669
UnstrandedReadsAssigned:2090046 PositiveStrandReadsAssigned:183166 NegativeStrandReadsAssigned:2162309
Dataset is classified negative stranded
MeadianReadLen=41 20thPercentileLength=41 echo kmer=37
SRR23332655 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR23332655-trimmed-pair1.fastq
                             SRR23332655-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,387,025 reads, 865,682 reads pseudoaligned
[quant] estimated average fragment length: 197.79
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 57 rounds

  4323 SRR23332655.ke.tsv
  4497 SRR23332655.se.tsv
  8820 total
==> SRR23332655.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	11.8307	111	1555.51
AAC73113	2463	2266.21	148	10.8274
AAC73114	933	736.262	50	11.259
AAC73115	1287	1090.21	122	18.5529
AAC73116	297	123.582	3	4.02462
AAC73117	777	580.397	12	3.42781
AAC73118	1431	1234.21	42	5.64185
AAC73119	954	757.236	317	69.4047
AAC73120	588	392.44	47	19.8557

==> SRR23332655.se.tsv <==
b0001	196
b0002	145
b0003	44
b0004	113
b0005	3
b0006	12
b0007	43
b0008	319
b0009	58
b0010	13
SRR23332655 completed mapping pipeline successfully
