Starting /dee2/code/volunteer_pipeline.sh SRR23332743
    current disk space = 1462926668
    free memory = 58822616 
SRR23332743 SRAfilesize
57ee896b76ba82bb30ef889be951edbb  SRR23332743.sra
SRR23332743.sra file validated
SRR23332743 is paired end
SRR23332743 is conventional basespace
SRR23332743 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332743_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.4325	37.0	37.0	37.0	37.0	37.0
2	36.4775	37.0	37.0	37.0	37.0	37.0
3	36.504	37.0	37.0	37.0	37.0	37.0
4	36.4375	37.0	37.0	37.0	37.0	37.0
5	36.444	37.0	37.0	37.0	37.0	37.0
6	36.1605	37.0	37.0	37.0	37.0	37.0
7	36.333	37.0	37.0	37.0	37.0	37.0
8	36.311	37.0	37.0	37.0	37.0	37.0
9	36.528	37.0	37.0	37.0	37.0	37.0
10	36.385	37.0	37.0	37.0	37.0	37.0
11	36.3975	37.0	37.0	37.0	37.0	37.0
12	36.3765	37.0	37.0	37.0	37.0	37.0
13	36.39	37.0	37.0	37.0	37.0	37.0
14	36.3555	37.0	37.0	37.0	37.0	37.0
15	36.4655	37.0	37.0	37.0	37.0	37.0
16	36.5205	37.0	37.0	37.0	37.0	37.0
17	36.275	37.0	37.0	37.0	37.0	37.0
18	36.384	37.0	37.0	37.0	37.0	37.0
19	36.286	37.0	37.0	37.0	37.0	37.0
20	36.3365	37.0	37.0	37.0	37.0	37.0
21	36.4545	37.0	37.0	37.0	37.0	37.0
22	36.17	37.0	37.0	37.0	37.0	37.0
23	36.276	37.0	37.0	37.0	37.0	37.0
24	36.2005	37.0	37.0	37.0	37.0	37.0
25	36.472	37.0	37.0	37.0	37.0	37.0
26	36.1485	37.0	37.0	37.0	37.0	37.0
27	36.3825	37.0	37.0	37.0	37.0	37.0
28	36.362	37.0	37.0	37.0	37.0	37.0
29	36.376	37.0	37.0	37.0	37.0	37.0
30	36.3545	37.0	37.0	37.0	37.0	37.0
31	36.1665	37.0	37.0	37.0	37.0	37.0
32	36.2505	37.0	37.0	37.0	37.0	37.0
33	36.363	37.0	37.0	37.0	37.0	37.0
34	36.3695	37.0	37.0	37.0	37.0	37.0
35	36.2865	37.0	37.0	37.0	37.0	37.0
36	36.076	37.0	37.0	37.0	37.0	37.0
37	36.182	37.0	37.0	37.0	37.0	37.0
38	36.2075	37.0	37.0	37.0	37.0	37.0
39	36.235	37.0	37.0	37.0	37.0	37.0
40	36.273	37.0	37.0	37.0	37.0	37.0
41	36.075	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	3.0
25	4.0
26	4.0
27	10.0
28	11.0
29	23.0
30	29.0
31	29.0
32	53.0
33	83.0
34	122.0
35	276.0
36	1348.0
37	2005.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.25	21.2	43.925	9.625
2	28.525	19.675	30.4	21.4
3	29.725	21.775	27.0	21.5
4	21.875	23.0	34.025	21.099999999999998
5	30.65	24.675	23.425	21.25
6	33.0	23.075000000000003	23.125	20.8
7	22.650000000000002	31.874999999999996	21.3	24.175
8	33.425	18.099999999999998	22.675	25.8
9	25.55	16.975	22.025	35.449999999999996
10	25.474999999999998	17.75	31.25	25.525
11	37.05	18.099999999999998	20.05	24.8
12	35.85	17.8	22.400000000000002	23.95
13	25.15	20.025000000000002	20.9	33.925
14	36.625	21.275	20.25	21.85
15	34.75	21.9	21.95	21.4
16	36.775000000000006	23.474999999999998	21.224999999999998	18.525
17	24.4	32.0	22.925	20.674999999999997
18	33.4	21.475	24.825	20.3
19	25.874999999999996	21.25	34.025	18.85
20	24.9	20.275000000000002	32.375	22.45
21	36.575	21.8	20.575	21.05
22	24.75	33.4	20.599999999999998	21.25
23	24.55	31.85	19.1	24.5
24	24.099999999999998	21.9	19.45	34.55
25	27.575	20.525	20.525	31.374999999999996
26	25.05	19.900000000000002	22.325	32.725
27	35.175	20.7	21.425	22.7
28	24.95	20.1	21.8	33.15
29	34.475	19.975	22.35	23.200000000000003
30	24.025	21.6	32.5	21.875
31	23.974999999999998	22.15	22.225	31.65
32	24.875	23.0	21.175	30.95
33	33.75	22.725	20.9	22.625
34	22.25	34.150000000000006	21.975	21.625
35	22.825	31.1	24.15	21.925
36	25.474999999999998	31.0	21.9	21.625
37	22.3	21.625	32.225	23.849999999999998
38	23.474999999999998	21.025	30.049999999999997	25.45
39	23.175	20.925	23.225	32.675
40	24.725	21.775	20.5	33.0
41	23.575	21.075	30.725	24.625
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	1.0
20	1.0
21	1.5
22	2.0
23	5.0
24	8.0
25	8.0
26	9.0
27	10.0
28	14.5
29	19.0
30	24.5
31	30.0
32	30.0
33	29.5
34	29.0
35	39.5
36	50.0
37	50.0
38	79.5
39	109.0
40	133.0
41	157.0
42	157.0
43	198.0
44	239.0
45	318.5
46	398.0
47	398.0
48	392.5
49	387.0
50	404.5
51	422.0
52	471.0
53	520.0
54	520.0
55	457.5
56	395.0
57	356.0
58	317.0
59	317.0
60	499.5
61	682.0
62	398.0
63	114.0
64	114.0
65	90.0
66	66.0
67	45.0
68	24.0
69	24.0
70	20.0
71	16.0
72	9.0
73	2.0
74	2.0
75	2.0
76	2.0
77	1.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.5
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.42744656917885	53.625
2	13.385826771653544	17.849999999999998
3	3.4120734908136483	6.825
4	1.2373453318335208	3.3000000000000003
5	0.6749156355455568	2.25
6	0.26246719160104987	1.05
7	0.14998125234345708	0.7000000000000001
8	0.11248593925759282	0.6
9	0.03749531308586427	0.22499999999999998
>10	0.26246719160104987	3.4750000000000005
>50	0.0	0.0
>100	0.03749531308586427	10.100000000000001
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCT	404	10.100000000000001	No Hit
GGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTA	40	1.0	No Hit
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTAC	36	0.8999999999999999	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCC	17	0.42500000000000004	No Hit
AGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGT	13	0.325	No Hit
GGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACAT	12	0.3	No Hit
GACTCTCAAGGAACGTTGAAGACGACGACGTTGATAGGCCG	11	0.27499999999999997	No Hit
AAAAAATGGCGCACAATGTGCGCCATTTTTCACTTCACAGG	10	0.25	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACGAGAGGACCGGGA	9	0.22499999999999998	No Hit
AAAAAATCCCCGCCAAATGGCAGGGATCTTAGATTCTGTGC	8	0.2	No Hit
TGCAGACTCCAATGAAGTCGGAATCGCTAGTAATCGTGGAT	8	0.2	No Hit
GTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTC	8	0.2	No Hit
TAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGA	7	0.17500000000000002	No Hit
AAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGAG	7	0.17500000000000002	No Hit
GGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAAC	7	0.17500000000000002	No Hit
AAAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGA	7	0.17500000000000002	No Hit
TTTCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTCC	6	0.15	No Hit
TTTATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGA	6	0.15	No Hit
TATGAACTCTTGGGGCTGAAGTAGGTCCCAAGGGTATGGCT	6	0.15	No Hit
TCTCGTACTAGGAGTGGACGCATCACTGGTGTTCGGGTTGT	6	0.15	No Hit
AAGTTTCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAG	6	0.15	No Hit
ATTCCGACTTCATGACTGGGGTGAAGTCGTAACAAGGTAAC	6	0.15	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCC	6	0.15	No Hit
TCAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCG	5	0.125	No Hit
TTCCGGATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGAC	5	0.125	No Hit
TCAGTTCTCCCTGACTCCTTGAGAGTCCTGAAGGAACGTTG	5	0.125	No Hit
GCTTGGTCAGCGCATTGCGCAGGTTACGCGTTACCGTGCTG	5	0.125	No Hit
TTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGTCG	5	0.125	No Hit
GTCTTCCGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAG	5	0.125	No Hit
TGAACTCTTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGT	5	0.125	No Hit
TTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGTCGTA	5	0.125	No Hit
GGATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGG	5	0.125	No Hit
ACAAAAGGGGTGAAACCACCCCTTCGTTAAAACTGTTCACT	5	0.125	No Hit
TACTAGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGC	5	0.125	No Hit
AAGAGAGTGGCTAACATCCTTATAGCCACTCTGTAGTATTA	5	0.125	No Hit
TCTCAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGT	5	0.125	No Hit
ATGATGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAGGCT	5	0.125	No Hit
TTACTAGCGATTCCCGGGCCTTGTACACACCGCCCGTCACA	5	0.125	No Hit
TTCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAG	5	0.125	No Hit
AAGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGA	5	0.125	No Hit
TGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.025	0.0	0.0	0.0	0.0
4	0.15	0.0	0.0	0.0	0.0
5	0.15	0.0	0.0	0.0	0.0
6	0.15	0.0	0.0	0.0	0.0
7	0.15	0.0	0.0	0.0	0.0
8	0.175	0.0	0.0	0.0	0.0
9	0.175	0.0	0.0	0.0	0.0
10	0.175	0.0	0.0	0.0	0.0
11	0.225	0.0	0.0	0.0	0.0
12	0.225	0.0	0.0	0.0	0.0
13	0.225	0.0	0.0	0.0	0.0
14	0.25	0.0	0.0	0.0	0.0
15	0.4	0.0	0.0	0.0	0.0
16	0.425	0.0	0.0	0.0	0.0
17	0.45	0.0	0.0	0.0	0.0
18	0.45	0.0	0.0	0.0	0.0
19	0.475	0.0	0.0	0.0	0.0
20	0.55	0.0	0.0	0.0	0.0
21	0.575	0.0	0.0	0.0	0.0
22	0.625	0.0	0.0	0.0	0.0
23	0.625	0.0	0.0	0.0	0.0
24	0.625	0.0	0.0	0.0	0.0
25	0.65	0.0	0.0	0.0	0.0
26	0.675	0.0	0.0	0.0	0.0
27	0.7	0.0	0.0	0.0	0.0
28	0.725	0.0	0.0	0.0	0.0
29	0.75	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR23332743 read2 length is 39-50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332743_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	39-50
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.309	11.0	11.0	37.0	11.0	37.0
2	21.7525	11.0	11.0	37.0	11.0	37.0
3	24.627	25.0	11.0	37.0	11.0	37.0
4	36.055	37.0	37.0	37.0	37.0	37.0
5	36.136	37.0	37.0	37.0	37.0	37.0
6	36.1015	37.0	37.0	37.0	37.0	37.0
7	35.8455	37.0	37.0	37.0	37.0	37.0
8	35.8765	37.0	37.0	37.0	37.0	37.0
9	35.851	37.0	37.0	37.0	37.0	37.0
10	35.906	37.0	37.0	37.0	37.0	37.0
11	35.922	37.0	37.0	37.0	37.0	37.0
12	35.935	37.0	37.0	37.0	37.0	37.0
13	36.0685	37.0	37.0	37.0	37.0	37.0
14	35.9955	37.0	37.0	37.0	37.0	37.0
15	35.777	37.0	37.0	37.0	37.0	37.0
16	35.966	37.0	37.0	37.0	37.0	37.0
17	36.1115	37.0	37.0	37.0	37.0	37.0
18	36.1635	37.0	37.0	37.0	37.0	37.0
19	36.0645	37.0	37.0	37.0	37.0	37.0
20	36.076	37.0	37.0	37.0	37.0	37.0
21	36.0315	37.0	37.0	37.0	37.0	37.0
22	36.225	37.0	37.0	37.0	37.0	37.0
23	36.1215	37.0	37.0	37.0	37.0	37.0
24	36.2115	37.0	37.0	37.0	37.0	37.0
25	36.013	37.0	37.0	37.0	37.0	37.0
26	36.085	37.0	37.0	37.0	37.0	37.0
27	36.025	37.0	37.0	37.0	37.0	37.0
28	36.014	37.0	37.0	37.0	37.0	37.0
29	36.2025	37.0	37.0	37.0	37.0	37.0
30	36.0345	37.0	37.0	37.0	37.0	37.0
31	36.0725	37.0	37.0	37.0	37.0	37.0
32	35.9555	37.0	37.0	37.0	37.0	37.0
33	35.969	37.0	37.0	37.0	37.0	37.0
34	36.0895	37.0	37.0	37.0	37.0	37.0
35	36.086	37.0	37.0	37.0	37.0	37.0
36	36.0645	37.0	37.0	37.0	37.0	37.0
37	36.026	37.0	37.0	37.0	37.0	37.0
38	36.095	37.0	37.0	37.0	37.0	37.0
39	36.0005	37.0	37.0	37.0	37.0	37.0
40	36.09804902451226	37.0	37.0	37.0	37.0	37.0
41	35.89194597298649	37.0	37.0	37.0	37.0	37.0
42	36.087587587587585	37.0	37.0	37.0	37.0	37.0
43	35.83654568210263	37.0	37.0	37.0	37.0	37.0
44	35.994972347913524	37.0	37.0	37.0	37.0	37.0
45	35.91991679667186	37.0	37.0	37.0	37.0	37.0
46	35.901858152316144	37.0	37.0	37.0	37.0	37.0
47	35.89978791092259	37.0	37.0	37.0	37.0	37.0
48	35.98445873526259	37.0	37.0	37.0	37.0	37.0
49	35.97205060311857	37.0	37.0	37.0	37.0	37.0
50	35.771517455928105	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	1.0
16	2.0
17	1.0
18	0.0
19	5.0
20	1.0
21	5.0
22	6.0
23	6.0
24	11.0
25	17.0
26	5.0
27	15.0
28	29.0
29	32.0
30	44.0
31	72.0
32	93.0
33	150.0
34	390.0
35	1762.0
36	1175.0
37	176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.55	29.025000000000002	28.425
2	31.4	41.75	11.1	15.75
3	25.15	44.474999999999994	15.85	14.524999999999999
4	79.725	7.249999999999999	5.075	7.95
5	64.525	17.325	10.8	7.35
6	55.50000000000001	19.525000000000002	12.15	12.825000000000001
7	35.125	38.875	12.975	13.025
8	43.575	19.650000000000002	13.950000000000001	22.825
9	19.400000000000002	40.525	26.325	13.750000000000002
10	38.675	22.3	17.775	21.25
11	29.125	38.2	17.474999999999998	15.2
12	23.474999999999998	25.8	25.1	25.624999999999996
13	37.65	15.525	26.6	20.225
14	36.05	15.975	25.5	22.475
15	28.449999999999996	18.9	41.4	11.25
16	44.85	19.675	23.875	11.600000000000001
17	25.674999999999997	33.875	18.475	21.975
18	15.625	39.15	26.650000000000002	18.575
19	19.025	19.950000000000003	34.725	26.3
20	16.875	22.475	32.275	28.375
21	18.099999999999998	41.525	15.5	24.875
22	40.1	27.950000000000003	14.799999999999999	17.150000000000002
23	23.400000000000002	28.675	21.25	26.674999999999997
24	22.1	24.6	14.7	38.6
25	36.0	18.65	14.85	30.5
26	31.740870435217612	20.66033016508254	15.157578789394696	32.44122061030515
27	37.475	24.45	20.974999999999998	17.1
28	34.226339509263894	25.488232348522782	25.162744116174263	15.12268402603906
29	24.625	16.7	43.575	15.1
30	20.180270405608415	16.825237856785176	48.37255883825738	14.621932899349025
31	19.7	23.325000000000003	38.15	18.825
32	34.426639959939905	14.071106659989985	19.90485728592889	31.59739609414121
33	22.475	14.025000000000002	16.175	47.325
34	27.04056084126189	16.42463695543315	24.211316975463195	32.323485227841765
35	14.725	30.75	22.975	31.55
36	14.089089089089088	38.41341341341341	29.504504504504503	17.992992992992992
37	17.7	44.375	14.35	23.575
38	21.4	20.349999999999998	12.525	45.725
39	37.35	17.2	14.424999999999999	31.025000000000002
40	24.262131065532767	19.334667333666832	19.85992996498249	36.54327163581791
41	18.434217108554275	39.74487243621811	26.513256628314156	15.307653826913455
42	20.045045045045047	27.227227227227228	31.906906906906908	20.82082082082082
43	31.08886107634543	22.052565707133915	18.1476846057572	28.71088861076345
44	37.631975867269986	15.309200603318251	19.331322272498745	27.727501256913023
45	19.006760270410815	16.770670826833072	32.579303172126885	31.643265730629228
46	28.448050248626018	20.308819680711856	33.10651661868621	18.136613451975922
47	31.511936339522546	15.941644562334217	27.16180371352785	25.384615384615383
48	27.331189710610932	24.839228295819936	25.214362272240088	22.615219721329048
49	31.01620029455081	13.667157584683357	38.46833578792342	16.848306332842416
50	28.309713100587626	15.036294503975112	28.65537504320774	27.99861735222952
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	2.0
26	4.0
27	5.0
28	8.0
29	16.5
30	22.0
31	26.0
32	34.5
33	42.5
34	48.0
35	47.5
36	46.0
37	49.0
38	56.5
39	76.0
40	89.0
41	102.0
42	128.0
43	153.0
44	171.0
45	182.0
46	192.5
47	194.5
48	189.5
49	200.0
50	223.0
51	382.0
52	548.0
53	704.5
54	852.5
55	791.5
56	693.5
57	547.5
58	400.5
59	285.0
60	184.5
61	113.5
62	57.0
63	55.0
64	48.5
65	35.0
66	26.5
67	21.0
68	13.0
69	9.0
70	7.0
71	4.0
72	2.5
73	3.0
74	2.5
75	1.0
76	0.0
77	1.5
78	3.0
79	2.0
80	1.0
81	1.0
82	1.0
83	1.5
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.05
27	0.0
28	0.15
29	0.0
30	0.15
31	0.0
32	0.15
33	0.0
34	0.15
35	0.0
36	0.1
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.05302226935312832
48	0.0
49	0.11768167107972932
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
39	2.0
40	0.0
41	2.0
42	1.0
43	17.0
44	132.0
45	25.0
46	49.0
47	40.0
48	333.0
49	506.0
50	2893.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.84600540331918	57.550000000000004
2	4.245465071401004	5.5
3	1.736781165573138	3.375
4	0.8490930142802007	2.1999999999999997
5	0.8876881512929371	2.875
6	0.8104978772674644	3.15
7	0.5403319181783096	2.45
8	0.23157082207641835	1.2
9	0.42454650714010034	2.475
>10	1.3122346584330373	13.625000000000002
>50	0.11578541103820918	5.6000000000000005
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	84	2.1	No Hit
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	76	1.9	No Hit
AAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	64	1.6	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	40	1.0	No Hit
AAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	34	0.8500000000000001	No Hit
CAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	32	0.8	No Hit
TAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	29	0.7250000000000001	No Hit
ATAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	21	0.525	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	19	0.475	No Hit
AGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	19	0.475	No Hit
CAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	19	0.475	No Hit
TGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	18	0.44999999999999996	No Hit
CGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	17	0.42500000000000004	No Hit
AATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	17	0.42500000000000004	No Hit
AACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	17	0.42500000000000004	No Hit
CAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	16	0.4	No Hit
TAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	16	0.4	No Hit
TGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	15	0.375	No Hit
ACAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
AATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	15	0.375	No Hit
TGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
CCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	12	0.3	No Hit
TGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	12	0.3	No Hit
AATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
CAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	11	0.27499999999999997	No Hit
CATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
AGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	11	0.27499999999999997	No Hit
AGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
CGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	11	0.27499999999999997	No Hit
CACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
AGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
AATGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	10	0.25	No Hit
CGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
TATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
AGAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	10	0.25	No Hit
AAAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	9	0.22499999999999998	No Hit
TTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
ATAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
CACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
TGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
CATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
TATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	9	0.22499999999999998	No Hit
TCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
AAAGGGTTAAACGAGGTACAGTTCTGTTTATGTGTGGCATTTTCAGTAAA	9	0.22499999999999998	No Hit
CGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
AGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
TCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
TGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
AACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
CCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
AAAGTGTCTTGCGAGGATAAGTGCATTATGAATATCTTACATATATGTGT	7	0.17500000000000002	No Hit
TCTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
ATAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
TTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTA	6	0.15	No Hit
AGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AAAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGT	6	0.15	No Hit
CGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TGGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
CGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
CCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
ATAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
AGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TCCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
ATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CTTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCT	5	0.125	No Hit
CGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CCGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
AGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	5	0.125	No Hit
AGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TAGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CGAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CGCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TCAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
ACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.025	0.0	0.0	0.0	0.0
11	0.025	0.0	0.0	0.0	0.0
12	0.075	0.0	0.0	0.0	0.0
13	0.125	0.0	0.0	0.0	0.0
14	0.125	0.0	0.0	0.0	0.0
15	0.125	0.0	0.0	0.0	0.0
16	0.175	0.0	0.0	0.0	0.0
17	0.175	0.0	0.0	0.0	0.0
18	0.2	0.0	0.0	0.0	0.0
19	0.225	0.0	0.0	0.0	0.0
20	0.225	0.0	0.0	0.0	0.0
21	0.3	0.0	0.0	0.0	0.0
22	0.35	0.0	0.0	0.0	0.0
23	0.4	0.0	0.0	0.0	0.0
24	0.45	0.0	0.0	0.0	0.0
25	0.45	0.0	0.0	0.0	0.0
26	0.475	0.0	0.0	0.0	0.0
27	0.475	0.0	0.0	0.0	0.0
28	0.475	0.0	0.0	0.0	0.0
29	0.55	0.0	0.0	0.0	0.0
30	0.575	0.0	0.0	0.0	0.0
31	0.6	0.0	0.0	0.0	0.0
32	0.6	0.0	0.0	0.0	0.0
33	0.6	0.0	0.0	0.0	0.0
34	0.65	0.0	0.0	0.0	0.0
35	0.675	0.0	0.0	0.0	0.0
36	0.675	0.0	0.0	0.0	0.0
37	0.7	0.0	0.0	0.0	0.0
38	0.725	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCATG	25	5.16215E-6	60.54386	44
GGTGCAT	25	1.163396E-5	53.092308	43
AGGTGCA	25	1.8353334E-5	49.300003	42
CAAGGTG	25	2.5802652E-5	46.635136	40
CCAAGGT	25	2.8011593E-5	46.013332	39
AACCCAA	25	3.847666E-5	43.683544	36
CCCAAGG	25	3.847666E-5	43.683544	38
ACCCAAG	25	3.847666E-5	43.683544	37
AAACCCA	25	4.154483E-5	43.1375	35
TCGACGG	25	4.154483E-5	43.1375	20
GAAACCC	25	4.154483E-5	43.1375	34
TCTGGAT	25	4.154483E-5	43.1375	13
TGATTCT	25	4.154483E-5	43.1375	9
GACGGGA	25	4.154483E-5	43.1375	22
GGATTTG	25	4.154483E-5	43.1375	26
TTCGACG	25	4.154483E-5	43.1375	19
TTCTGGA	25	4.154483E-5	43.1375	12
TGCGAAA	25	4.154483E-5	43.1375	31
GGATTCG	25	4.154483E-5	43.1375	16
GCGAAAC	25	4.154483E-5	43.1375	32
>>END_MODULE
Read 434790 spots for SRR23332743.sra
Written 434790 spots for SRR23332743.sra
Read 434797 spots for SRR23332743.sra
Written 434797 spots for SRR23332743.sra
Read 434790 spots for SRR23332743.sra
Written 434790 spots for SRR23332743.sra
Read 434790 spots for SRR23332743.sra
Written 434790 spots for SRR23332743.sra
Read 434790 spots for SRR23332743.sra
Written 434790 spots for SRR23332743.sra
Read 434790 spots for SRR23332743.sra
Written 434790 spots for SRR23332743.sra
Read 434790 spots for SRR23332743.sra
Written 434790 spots for SRR23332743.sra
Read 434790 spots for SRR23332743.sra
Written 434790 spots for SRR23332743.sra
SRR ids: ['SRR23332743.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_1lhkkkxe
SRR23332743.sra spots: 3478327
blocks: [[1, 434790], [434791, 869580], [869581, 1304370], [1304371, 1739160], [1739161, 2173950], [2173951, 2608740], [2608741, 3043530], [3043531, 3478327]]
SRR23332743 file size 422432
SRR23332743 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR23332743 SRR23332743_1.fastq SRR23332743_2.fastq
Input file:	SRR23332743_1.fastq
Paired file:	SRR23332743_2.fastq
trimmed:	SRR23332743-trimmed-pair1.fastq, SRR23332743-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 03:03:59 2026 >> started

Wed Mar 25 03:04:01 2026 >> done (1.938s)
3478327 read pairs processed; of these:
    852 ( 0.02%) short read pairs filtered out after trimming by size control
     36 ( 0.00%) empty read pairs filtered out after trimming by size control
3477439 (99.97%) read pairs available; of these:
  20920 ( 0.60%) trimmed read pairs available after processing
3456519 (99.40%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    125	  0.00%
 19	    245	  0.01%
 20	    450	  0.01%
 21	    256	  0.01%
 22	    242	  0.01%
 23	    274	  0.01%
 24	    336	  0.01%
 25	    496	  0.01%
 26	    414	  0.01%
 27	    450	  0.01%
 28	    346	  0.01%
 29	    634	  0.02%
 30	    472	  0.01%
 31	    567	  0.02%
 32	    699	  0.02%
 33	    800	  0.02%
 34	   1090	  0.03%
 35	   1053	  0.03%
 36	   1222	  0.04%
 37	   1160	  0.03%
 38	   1758	  0.05%
 39	   2738	  0.08%
 40	   2751	  0.08%
 41	   3508	  0.10%
 42	 121040	  3.48%
 43	  64124	  1.84%
 44	 289097	  8.31%
 45	2981092	 85.73%
3477439 reads passed initial QC


criterion=sequence-density
sequence-density=1.16
sequence-density-rank=1
fanout-score=2.51
fanout-score-rank=19
prefix-density=1.17
prefix-fanout=2.5
sequence=ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG


criterion=fanout-score
sequence-density=0.10
sequence-density-rank=31
fanout-score=7.37
fanout-score-rank=1
prefix-density=0.72
prefix-fanout=1.0
sequence=TAGCGATTCCGACTTCATG


criterion=sequence-density
sequence-density=13.07
sequence-density-rank=1
fanout-score=21.20
fanout-score-rank=4
prefix-density=10.15
prefix-fanout=21.2
sequence=GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGGACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCTCACGGTT


criterion=fanout-score
sequence-density=0.71
sequence-density-rank=12
fanout-score=156.23
fanout-score-rank=1
prefix-density=10.22
prefix-fanout=10.8
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTAGAGATTGT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG -y GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGGACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCTCACGGTT -o SRR23332743 SRR23332743_1.fastq SRR23332743_2.fastq
Input file:	SRR23332743_1.fastq
Paired file:	SRR23332743_2.fastq
trimmed:	SRR23332743-trimmed-pair1.fastq, SRR23332743-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG
-- paired 3' end adapter sequence (-y):	GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 03:04:09 2026 >> started

Wed Mar 25 03:04:10 2026 >> done (1.653s)
2608079 read pairs processed; of these:
  19664 ( 0.75%) short read pairs filtered out after trimming by size control
   3111 ( 0.12%) empty read pairs filtered out after trimming by size control
2585304 (99.13%) read pairs available; of these:
    760 ( 0.03%) trimmed read pairs available after processing
2584544 (99.97%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     93	  0.00%
 19	    190	  0.01%
 20	    354	  0.01%
 21	    199	  0.01%
 22	    190	  0.01%
 23	    209	  0.01%
 24	    257	  0.01%
 25	    375	  0.01%
 26	    314	  0.01%
 27	    324	  0.01%
 28	    267	  0.01%
 29	    468	  0.02%
 30	    332	  0.01%
 31	    422	  0.02%
 32	    537	  0.02%
 33	    607	  0.02%
 34	    828	  0.03%
 35	    782	  0.03%
 36	    930	  0.04%
 37	    864	  0.03%
 38	   1345	  0.05%
 39	   2039	  0.08%
 40	   2050	  0.08%
 41	   2630	  0.10%
 42	  90552	  3.50%
 43	  48109	  1.86%
 44	 206164	  7.97%
 45	2223873	 86.02%


criterion=sequence-density
sequence-density=1.08
sequence-density-rank=1
fanout-score=2.61
fanout-score-rank=18
prefix-density=1.12
prefix-fanout=2.5
sequence=ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG


criterion=fanout-score
sequence-density=0.10
sequence-density-rank=30
fanout-score=7.02
fanout-score-rank=1
prefix-density=0.69
prefix-fanout=1.0
sequence=TAGCGATTCCGACTTCATG


criterion=sequence-density
sequence-density=12.49
sequence-density-rank=1
fanout-score=21.99
fanout-score-rank=4
prefix-density=9.94
prefix-fanout=22.0
sequence=GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGGACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCTCACGGTT


criterion=fanout-score
sequence-density=0.72
sequence-density-rank=12
fanout-score=156.42
fanout-score-rank=1
prefix-density=10.26
prefix-fanout=10.9
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTAGAGATTGT
SRR23332743 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 25 03:04:26
                             Started mapping on |	Mar 25 03:04:26
                                    Finished on |	Mar 25 03:06:05
       Mapping speed, Million of reads per hour |	125.62

                          Number of input reads |	3454664
                      Average input read length |	90
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1999672
                        Uniquely mapped reads % |	57.88%
                          Average mapped length |	87.88
                       Number of splices: Total |	100837
            Number of splices: Annotated (sjdb) |	409
                       Number of splices: GT/AG |	92333
                       Number of splices: GC/AG |	2164
                       Number of splices: AT/AC |	241
               Number of splices: Non-canonical |	6099
                      Mismatch rate per base, % |	0.26%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.40
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	662655
             % of reads mapped to multiple loci |	19.18%
        Number of reads mapped to too many loci |	606634
             % of reads mapped to too many loci |	17.56%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.06%
                     % of reads unmapped: other |	0.32%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	792337	792337	792337
N_multimapping	662655	662655	662655
N_noFeature	53749	1854766	116646
N_ambiguous	281043	24540	180735
UnstrandedReadsAssigned:1664880 PositiveStrandReadsAssigned:120366 NegativeStrandReadsAssigned:1702291
Dataset is classified negative stranded
MeadianReadLen=41 20thPercentileLength=41 echo kmer=37
SRR23332743 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR23332743-trimmed-pair1.fastq
                             SRR23332743-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,454,664 reads, 971,735 reads pseudoaligned
[quant] estimated average fragment length: 225.442
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 52 rounds

  4323 SRR23332743.ke.tsv
  4497 SRR23332743.se.tsv
  8820 total
==> SRR23332743.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	13.1864	58	1081.22
AAC73113	2463	2238.56	136	14.9342
AAC73114	933	708.604	75	26.0177
AAC73115	1287	1062.56	144	33.3136
AAC73116	297	108.092	29	65.9503
AAC73117	777	552.855	49	21.787
AAC73118	1431	1206.56	28	5.70455
AAC73119	954	729.587	1976	665.766
AAC73120	588	365.749	166	111.567

==> SRR23332743.se.tsv <==
b0001	91
b0002	135
b0003	58
b0004	135
b0005	29
b0006	56
b0007	29
b0008	1988
b0009	207
b0010	16
SRR23332743 completed mapping pipeline successfully
