Starting /dee2/code/volunteer_pipeline.sh SRR23332606
    current disk space = 1440077380
    free memory = 57414580 
SRR23332606 SRAfilesize
5e26af7b6731cb35499cc73407ca7c35  SRR23332606.sra
SRR23332606.sra file validated
SRR23332606 is paired end
SRR23332606 is conventional basespace
SRR23332606 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332606_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.387	37.0	37.0	37.0	37.0	37.0
2	36.4015	37.0	37.0	37.0	37.0	37.0
3	36.523	37.0	37.0	37.0	37.0	37.0
4	36.289	37.0	37.0	37.0	37.0	37.0
5	36.389	37.0	37.0	37.0	37.0	37.0
6	36.3425	37.0	37.0	37.0	37.0	37.0
7	36.2605	37.0	37.0	37.0	37.0	37.0
8	36.1345	37.0	37.0	37.0	37.0	37.0
9	36.471	37.0	37.0	37.0	37.0	37.0
10	36.4385	37.0	37.0	37.0	37.0	37.0
11	36.408	37.0	37.0	37.0	37.0	37.0
12	36.3885	37.0	37.0	37.0	37.0	37.0
13	36.383	37.0	37.0	37.0	37.0	37.0
14	36.366	37.0	37.0	37.0	37.0	37.0
15	36.3215	37.0	37.0	37.0	37.0	37.0
16	36.4635	37.0	37.0	37.0	37.0	37.0
17	36.2205	37.0	37.0	37.0	37.0	37.0
18	36.2145	37.0	37.0	37.0	37.0	37.0
19	36.2795	37.0	37.0	37.0	37.0	37.0
20	36.304	37.0	37.0	37.0	37.0	37.0
21	36.3455	37.0	37.0	37.0	37.0	37.0
22	36.2925	37.0	37.0	37.0	37.0	37.0
23	36.283	37.0	37.0	37.0	37.0	37.0
24	36.343	37.0	37.0	37.0	37.0	37.0
25	36.4215	37.0	37.0	37.0	37.0	37.0
26	36.23	37.0	37.0	37.0	37.0	37.0
27	36.319	37.0	37.0	37.0	37.0	37.0
28	36.3145	37.0	37.0	37.0	37.0	37.0
29	36.2955	37.0	37.0	37.0	37.0	37.0
30	36.305	37.0	37.0	37.0	37.0	37.0
31	36.323	37.0	37.0	37.0	37.0	37.0
32	36.3425	37.0	37.0	37.0	37.0	37.0
33	36.4085	37.0	37.0	37.0	37.0	37.0
34	36.2075	37.0	37.0	37.0	37.0	37.0
35	36.2615	37.0	37.0	37.0	37.0	37.0
36	36.098	37.0	37.0	37.0	37.0	37.0
37	36.1055	37.0	37.0	37.0	37.0	37.0
38	36.3225	37.0	37.0	37.0	37.0	37.0
39	36.059	37.0	37.0	37.0	37.0	37.0
40	36.2565	37.0	37.0	37.0	37.0	37.0
41	36.193	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	0.0
25	3.0
26	7.0
27	13.0
28	13.0
29	14.0
30	34.0
31	37.0
32	53.0
33	85.0
34	117.0
35	281.0
36	1345.0
37	1997.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.400000000000002	16.55	53.6	7.449999999999999
2	37.55	15.875	28.225	18.35
3	37.525	18.0	27.075	17.4
4	18.275	21.05	41.949999999999996	18.725
5	36.449999999999996	21.05	24.2	18.3
6	38.2	20.7	20.8	20.3
7	19.05	39.725	19.3	21.925
8	42.699999999999996	17.549999999999997	18.75	21.0
9	22.925	13.750000000000002	19.35	43.974999999999994
10	22.725	13.850000000000001	40.125	23.3
11	41.675000000000004	17.0	18.45	22.875
12	42.449999999999996	16.35	20.75	20.45
13	22.5	17.775	17.974999999999998	41.75
14	41.825	19.425	18.35	20.4
15	42.325	16.975	21.224999999999998	19.475
16	44.525	17.424999999999997	20.424999999999997	17.625
17	22.425	39.825	19.925	17.825
18	42.95	18.975	21.275	16.8
19	22.125	20.225	39.925	17.724999999999998
20	21.575	19.725	38.3	20.4
21	42.075	18.85	18.55	20.525
22	22.425	37.55	19.825	20.200000000000003
23	22.15	38.324999999999996	19.400000000000002	20.125
24	21.6	19.3	18.9	40.2
25	24.05	16.825000000000003	19.425	39.7
26	21.275	17.325	22.825	38.574999999999996
27	42.449999999999996	17.474999999999998	20.775	19.3
28	20.849999999999998	17.325	21.25	40.575
29	41.975	17.1	21.3	19.625
30	20.075000000000003	18.35	43.375	18.2
31	20.200000000000003	18.625	21.45	39.725
32	20.925	20.025000000000002	20.275000000000002	38.775
33	40.150000000000006	20.875	21.95	17.025000000000002
34	21.099999999999998	38.975	19.025	20.9
35	19.525000000000002	40.2	21.3	18.975
36	19.55	39.025	20.825	20.599999999999998
37	20.3	20.349999999999998	39.800000000000004	19.55
38	22.825	18.7	39.050000000000004	19.425
39	19.525000000000002	20.45	20.325	39.7
40	20.075000000000003	20.525	18.7	40.699999999999996
41	21.4	19.475	39.45	19.675
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	3.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	2.0
20	2.0
21	2.0
22	2.0
23	2.5
24	3.0
25	3.0
26	3.0
27	3.0
28	15.5
29	28.0
30	25.5
31	23.0
32	23.0
33	29.0
34	35.0
35	40.5
36	46.0
37	46.0
38	71.0
39	96.0
40	160.0
41	224.0
42	224.0
43	242.0
44	260.0
45	266.5
46	273.0
47	273.0
48	334.5
49	396.0
50	399.5
51	403.0
52	417.5
53	432.0
54	432.0
55	369.0
56	306.0
57	290.5
58	275.0
59	275.0
60	650.5
61	1026.0
62	559.0
63	92.0
64	92.0
65	63.0
66	34.0
67	25.0
68	16.0
69	16.0
70	12.5
71	9.0
72	8.0
73	7.0
74	3.5
75	0.0
76	0.0
77	0.5
78	1.0
79	1.0
80	1.0
81	1.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.7
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.25673249551167	42.475
2	16.202872531418315	18.05
3	4.174147217235189	6.9750000000000005
4	1.2118491921005385	2.7
5	0.718132854578097	2.0
6	0.4488330341113106	1.5
7	0.2244165170556553	0.8750000000000001
8	0.08976660682226212	0.4
9	0.13464991023339318	0.675
>10	0.49371633752244165	4.55
>50	0.0	0.0
>100	0.0	0.0
>500	0.04488330341113106	19.8
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCT	792	19.8	No Hit
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTAC	27	0.675	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCC	24	0.6	No Hit
GGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAAC	22	0.5499999999999999	No Hit
GGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTA	21	0.525	No Hit
AAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGAG	19	0.475	No Hit
AGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGT	14	0.35000000000000003	No Hit
TTCCTACATCCTCGGTACTACATGCTTAGTCAGTCTTTACA	13	0.325	No Hit
GGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACAT	11	0.27499999999999997	No Hit
GCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACA	11	0.27499999999999997	No Hit
TCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGACT	10	0.25	No Hit
AAAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGA	10	0.25	No Hit
GGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAACC	9	0.22499999999999998	No Hit
TTTCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTCC	9	0.22499999999999998	No Hit
TTTGAGTTCCCGGGCCTTGTACACACCGCCCGTCACACCAT	9	0.22499999999999998	No Hit
TTTATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGA	8	0.2	No Hit
TACATCCTCGGTACTACATGCTTAGTCAGTCTTTACATTCG	8	0.2	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACGAGAGGACCGGGA	7	0.17500000000000002	No Hit
AGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAA	7	0.17500000000000002	No Hit
TTTTTTGCGTACTGTCAGAGCACTGATTTCTCTGACAGTAC	7	0.17500000000000002	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.17500000000000002	No Hit
AAAAATGGCGCACAATGTGCGCCATTTTTCACTTCACAGGT	7	0.17500000000000002	No Hit
GCTACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACT	6	0.15	No Hit
TTGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCC	6	0.15	No Hit
TACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACTGG	6	0.15	No Hit
TCCTACATCCTCGGTACTACATGCTTAGTCAGTCTTTACAT	6	0.15	No Hit
CTTCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAA	6	0.15	No Hit
TTTTTTTGCGTACTGTCAGAGCACTGATTTCTCTGACAGTA	6	0.15	No Hit
TGAGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGG	6	0.15	No Hit
TTCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAG	6	0.15	No Hit
GGCGGTGTGTACAAGGTAACCGTAGGGGAACCTGCGGTTGG	6	0.15	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCC	6	0.15	No Hit
GGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTC	5	0.125	No Hit
GCGGGAGTTGAACCCGCGTCCGAAATTCCTACATCCTCGGT	5	0.125	No Hit
TCCTCGGTACTACATGCTTAGTCAGTCTTTACATTCGCTTG	5	0.125	No Hit
GGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAA	5	0.125	No Hit
TACTTCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTG	5	0.125	No Hit
AAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTG	5	0.125	No Hit
TTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACGTTGAAGA	5	0.125	No Hit
TGCTCTCGCGAGGTCGGAATCGCTAGTAATCGTGGATCAGA	5	0.125	No Hit
TTTCTTTTCCTCGGGGCAGGGTGAGTCGACCCCTAAGGCGA	5	0.125	No Hit
TTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCAC	5	0.125	No Hit
TTATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGAA	5	0.125	No Hit
ACAAACGGCACGACACAGCTGTCGTACCGTTATTGCTTATT	5	0.125	No Hit
AAGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGA	5	0.125	No Hit
TGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACA	5	0.125	No Hit
AGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCCA	5	0.125	No Hit
ATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.025	0.0	0.0	0.0	0.0
5	0.025	0.0	0.0	0.0	0.0
6	0.025	0.0	0.0	0.0	0.0
7	0.025	0.0	0.0	0.0	0.0
8	0.025	0.0	0.0	0.0	0.0
9	0.025	0.0	0.0	0.0	0.0
10	0.025	0.0	0.0	0.0	0.0
11	0.025	0.0	0.0	0.0	0.0
12	0.025	0.0	0.0	0.0	0.0
13	0.025	0.0	0.0	0.0	0.0
14	0.025	0.0	0.0	0.0	0.0
15	0.025	0.0	0.0	0.0	0.0
16	0.025	0.0	0.0	0.0	0.0
17	0.025	0.0	0.0	0.0	0.0
18	0.05	0.0	0.0	0.0	0.0
19	0.075	0.0	0.0	0.0	0.0
20	0.125	0.0	0.0	0.0	0.0
21	0.175	0.0	0.0	0.0	0.0
22	0.175	0.0	0.0	0.0	0.0
23	0.175	0.0	0.0	0.0	0.0
24	0.2	0.0	0.0	0.0	0.0
25	0.2	0.0	0.0	0.0	0.0
26	0.2	0.0	0.0	0.0	0.0
27	0.325	0.0	0.0	0.0	0.0
28	0.375	0.0	0.0	0.0	0.0
29	0.425	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGAGCT	90	0.0	35.000004	4
GGTGGAG	90	0.0	35.000004	2
TGGTGGA	90	0.0	35.000004	1
GTGGAGC	90	0.0	35.000004	3
GCTGGCG	100	0.0	31.499998	8
GAGCTGG	100	0.0	31.499998	6
TGGCGGG	100	0.0	31.499998	10
AGCTGGC	100	0.0	31.499998	7
GGAGCTG	100	0.0	31.499998	5
GGCGGGA	100	0.0	31.499998	11
GCGGGAG	105	0.0	30.000002	12
GTTGAAC	105	0.0	30.000002	18
GAAATTC	105	0.0	30.000002	33
AAATTCC	105	0.0	30.000002	34
AATTCCT	105	0.0	30.000002	35
GTCCGAA	105	0.0	30.000002	29
AACCCGC	105	0.0	30.000002	22
ACCCGCG	105	0.0	30.000002	23
GGGAGTT	105	0.0	30.000002	14
GAGTTGA	105	0.0	30.000002	16
>>END_MODULE
SRR23332606 read2 length is 42-50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332606_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	42-50
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.7465	11.0	11.0	37.0	11.0	37.0
2	22.2345	25.0	11.0	37.0	11.0	37.0
3	24.751	25.0	11.0	37.0	11.0	37.0
4	36.022	37.0	37.0	37.0	37.0	37.0
5	36.1695	37.0	37.0	37.0	37.0	37.0
6	36.095	37.0	37.0	37.0	37.0	37.0
7	35.7135	37.0	37.0	37.0	37.0	37.0
8	35.881	37.0	37.0	37.0	37.0	37.0
9	35.8355	37.0	37.0	37.0	37.0	37.0
10	36.0175	37.0	37.0	37.0	37.0	37.0
11	35.962	37.0	37.0	37.0	37.0	37.0
12	35.9025	37.0	37.0	37.0	37.0	37.0
13	35.9915	37.0	37.0	37.0	37.0	37.0
14	36.1385	37.0	37.0	37.0	37.0	37.0
15	35.8005	37.0	37.0	37.0	37.0	37.0
16	36.095	37.0	37.0	37.0	37.0	37.0
17	36.079	37.0	37.0	37.0	37.0	37.0
18	36.1065	37.0	37.0	37.0	37.0	37.0
19	36.065	37.0	37.0	37.0	37.0	37.0
20	36.0365	37.0	37.0	37.0	37.0	37.0
21	36.036	37.0	37.0	37.0	37.0	37.0
22	36.1425	37.0	37.0	37.0	37.0	37.0
23	36.108	37.0	37.0	37.0	37.0	37.0
24	36.084	37.0	37.0	37.0	37.0	37.0
25	36.0475	37.0	37.0	37.0	37.0	37.0
26	35.9845	37.0	37.0	37.0	37.0	37.0
27	35.93125	37.0	37.0	37.0	37.0	37.0
28	35.8645	37.0	37.0	37.0	37.0	37.0
29	36.1265	37.0	37.0	37.0	37.0	37.0
30	35.9415	37.0	37.0	37.0	37.0	37.0
31	35.915	37.0	37.0	37.0	37.0	37.0
32	35.92625	37.0	37.0	37.0	37.0	37.0
33	36.0425	37.0	37.0	37.0	37.0	37.0
34	36.26475	37.0	37.0	37.0	37.0	37.0
35	36.0935	37.0	37.0	37.0	37.0	37.0
36	36.042	37.0	37.0	37.0	37.0	37.0
37	36.0725	37.0	37.0	37.0	37.0	37.0
38	35.9155	37.0	37.0	37.0	37.0	37.0
39	36.0145	37.0	37.0	37.0	37.0	37.0
40	36.0065	37.0	37.0	37.0	37.0	37.0
41	35.7275	37.0	37.0	37.0	37.0	37.0
42	35.8895	37.0	37.0	37.0	37.0	37.0
43	35.75925925925926	37.0	37.0	37.0	37.0	37.0
44	35.956675062972295	37.0	37.0	37.0	37.0	37.0
45	35.86732570239334	37.0	37.0	37.0	37.0	37.0
46	35.93712181004999	37.0	37.0	37.0	37.0	37.0
47	36.00161030595813	37.0	37.0	37.0	37.0	37.0
48	36.11105047748977	37.0	37.0	37.0	37.0	37.0
49	35.92580354460799	37.0	37.0	37.0	37.0	37.0
50	35.72791770131252	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	3.0
20	2.0
21	6.0
22	12.0
23	10.0
24	10.0
25	14.0
26	13.0
27	15.0
28	28.0
29	23.0
30	38.0
31	60.0
32	90.0
33	166.0
34	393.0
35	1769.0
36	1153.0
37	192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	39.125	29.575000000000003	31.3
2	30.599999999999998	40.699999999999996	13.15	15.55
3	27.625	40.525	16.7	15.15
4	79.10000000000001	7.6	4.675	8.625
5	65.14999999999999	16.725	10.375	7.75
6	57.325	20.45	10.6	11.625
7	39.65	36.4	10.875	13.075000000000001
8	38.275	20.974999999999998	13.475000000000001	27.275
9	19.7	37.45	29.299999999999997	13.55
10	42.025	24.15	17.5	16.325
11	28.449999999999996	41.425	16.175	13.950000000000001
12	22.375	27.0	29.875	20.75
13	34.4	15.275	31.525	18.8
14	32.074999999999996	17.299999999999997	23.575	27.05
15	26.8	19.55	42.699999999999996	10.95
16	44.775	22.775000000000002	20.65	11.799999999999999
17	29.65	32.175	18.775	19.400000000000002
18	17.1	39.125	26.275	17.5
19	18.6	20.3	35.4	25.7
20	15.8	23.9	32.85	27.450000000000003
21	19.475	39.45	13.225000000000001	27.85
22	38.1	28.999999999999996	14.099999999999998	18.8
23	21.65	34.125	17.125	27.1
24	22.95	23.200000000000003	12.875	40.975
25	42.075	17.95	12.775	27.200000000000003
26	35.075	21.875	13.05	30.0
27	39.23480870217554	27.081770442610654	18.179544886221557	15.503875968992247
28	31.615807903951975	29.914957478739368	25.062531265632813	13.406703351675839
29	24.575	16.650000000000002	44.275	14.499999999999998
30	19.23461730865433	17.483741870935468	49.44972486243122	13.83191595797899
31	19.15	21.275	39.975	19.6
32	35.57668251188391	14.886164623467602	18.413810357768327	31.123342506880157
33	22.05	14.774999999999999	15.375	47.8
34	29.246935201401055	17.538153615211407	21.866399799849887	31.34851138353765
35	14.249999999999998	36.075	21.825	27.85
36	13.856928464232116	40.920460230115054	27.5887943971986	17.63381690845423
37	17.299999999999997	45.800000000000004	13.175	23.724999999999998
38	20.75	18.675	12.049999999999999	48.525
39	32.95	17.75	12.025	37.275000000000006
40	22.875	21.0	18.65	37.475
41	18.525	39.925	26.700000000000003	14.85
42	20.825	34.5	26.0	18.675
43	34.63463463463464	23.523523523523522	16.116116116116117	25.725725725725724
44	39.37027707808564	15.08816120906801	18.564231738035264	26.97732997481108
45	19.458896982310094	16.207075962539022	34.46930280957336	29.864724245577523
46	32.96500920810313	17.67955801104972	30.14996053670087	19.205472244146275
47	28.931830381105744	15.727321524422974	25.738056897477186	29.602791196994094
48	26.193724420190996	29.16780354706685	25.2387448840382	19.399727148703956
49	26.420198376916144	13.55575593627893	43.52269311692215	16.501352569882776
50	35.29620432777581	14.686058886129832	26.78254700248315	23.23518978361121
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	3.5
6	6.0
7	3.5
8	1.0
9	1.0
10	1.0
11	1.0
12	1.0
13	0.5
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	1.0
20	1.0
21	2.0
22	3.0
23	2.0
24	1.0
25	2.0
26	4.0
27	8.0
28	11.0
29	14.5
30	20.5
31	32.0
32	38.0
33	34.0
34	41.0
35	64.5
36	82.5
37	80.0
38	75.5
39	83.0
40	99.5
41	116.5
42	119.5
43	115.0
44	114.0
45	127.5
46	149.0
47	165.0
48	179.5
49	197.5
50	209.0
51	389.5
52	591.5
53	729.5
54	857.0
55	800.0
56	710.0
57	567.0
58	411.0
59	278.5
60	165.5
61	111.0
62	67.5
63	56.0
64	46.5
65	30.0
66	15.0
67	13.0
68	11.5
69	8.0
70	5.0
71	2.5
72	2.0
73	3.0
74	2.0
75	1.0
76	1.0
77	1.5
78	2.0
79	3.0
80	4.0
81	2.5
82	1.0
83	1.5
84	2.0
85	2.0
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	2.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.025
28	0.05
29	0.0
30	0.05
31	0.0
32	0.075
33	0.0
34	0.075
35	0.0
36	0.05
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.06007810153199159
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
42	4.0
43	26.0
44	126.0
45	43.0
46	75.0
47	61.0
48	336.0
49	510.0
50	2819.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.824999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.62435336251492	55.05
2	4.854755272582571	6.1
3	2.0692399522483087	3.9
4	1.15399920413848	2.9000000000000004
5	0.9152407481098289	2.875
6	0.47751691205730207	1.7999999999999998
7	0.2785515320334262	1.225
8	0.4377238360525269	2.1999999999999997
9	0.5173099880620772	2.9250000000000003
>10	1.591723040191007	16.525000000000002
>50	0.03979307600477517	1.425
>100	0.03979307600477517	3.075
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	123	3.075	No Hit
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	57	1.425	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	36	0.8999999999999999	No Hit
CAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	36	0.8999999999999999	No Hit
AAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	36	0.8999999999999999	No Hit
AAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	34	0.8500000000000001	No Hit
TAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	31	0.775	No Hit
AATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	27	0.675	No Hit
AACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	25	0.625	No Hit
CGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	21	0.525	No Hit
TAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	19	0.475	No Hit
CACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	18	0.44999999999999996	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	18	0.44999999999999996	No Hit
ACAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	18	0.44999999999999996	No Hit
CGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
CGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	16	0.4	No Hit
CAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	15	0.375	No Hit
TATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
CCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
CGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
CCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
CGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
TACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
CAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	13	0.325	No Hit
TGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
CGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
TATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
ACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
CCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
CGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
CTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
TAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
CAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	11	0.27499999999999997	No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
TGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	10	0.25	No Hit
TGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
AACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
CGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
CCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
AGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
CCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CCCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
AGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
TACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
AGACACGGTACTAATGAACCGTGAGGCTTAACCTTACTCGGTAC	9	0.22499999999999998	No Hit
CTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
AGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
AGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
AAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
ATAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
AAAGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTG	8	0.2	No Hit
CGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
TGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TATGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
CATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
TGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TTTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
AATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTA	7	0.17500000000000002	No Hit
TTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCT	7	0.17500000000000002	No Hit
TAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
ACTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
ACAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
AAAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAT	6	0.15	No Hit
CCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
AAAGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	6	0.15	No Hit
TTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
AAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TTGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
AACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TTCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TCGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TTTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AAAGGGCAATGATAAAAGGTGGCAAAAATGAATGTTTCCAGTAGAACTGT	5	0.125	No Hit
ATTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AAAGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCC	5	0.125	No Hit
ATAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
ACGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TTTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.025	0.0	0.0	0.0	0.0
11	0.025	0.0	0.0	0.0	0.0
12	0.025	0.0	0.0	0.0	0.0
13	0.025	0.0	0.0	0.0	0.0
14	0.025	0.0	0.0	0.0	0.0
15	0.025	0.0	0.0	0.0	0.0
16	0.025	0.0	0.0	0.0	0.0
17	0.025	0.0	0.0	0.0	0.0
18	0.025	0.0	0.0	0.0	0.0
19	0.025	0.0	0.0	0.0	0.0
20	0.025	0.0	0.0	0.0	0.0
21	0.025	0.0	0.0	0.0	0.0
22	0.05	0.0	0.0	0.0	0.0
23	0.075	0.0	0.0	0.0	0.0
24	0.075	0.0	0.0	0.0	0.0
25	0.075	0.0	0.0	0.0	0.0
26	0.1	0.0	0.0	0.0	0.0
27	0.125	0.0	0.0	0.0	0.0
28	0.125	0.0	0.0	0.0	0.0
29	0.2	0.0	0.0	0.0	0.0
30	0.275	0.0	0.0	0.0	0.0
31	0.275	0.0	0.0	0.0	0.0
32	0.3	0.0	0.0	0.0	0.0
33	0.35	0.0	0.0	0.0	0.0
34	0.35	0.0	0.0	0.0	0.0
35	0.375	0.0	0.0	0.0	0.0
36	0.425	0.0	0.0	0.0	0.0
37	0.475	0.0	0.0	0.0	0.0
38	0.475	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGGGAG	30	2.263725E-6	43.0625	2
AAAGGGA	25	4.1974152E-5	43.0625	1
CCTTGTT	60	1.1837801E-7	41.86632	44
GTGCATG	90	1.3569661E-9	35.885418	44
AGGGAGA	45	8.71838E-7	33.493057	3
ACCTTGT	65	6.7180736E-8	32.615383	43
TACCTTG	65	1.1749398E-7	30.72464	42
GAGAGGA	50	1.9880408E-6	30.143751	6
TTACCTT	65	1.5335718E-7	29.859154	41
CGGGAGA	30	0.0056614713	28.708332	3
GTTACCT	65	2.5514055E-7	28.266665	40
GGAGAGG	55	4.1830826E-6	27.403408	5
GGGAGAG	55	4.1830826E-6	27.403408	4
CCAACCG	65	4.6316563E-7	26.5	19
AACCGCA	65	4.6316563E-7	26.5	21
ATCCAAC	65	4.6316563E-7	26.5	17
CAACCGC	65	4.6316563E-7	26.5	20
ACCGCAG	65	4.6316563E-7	26.5	22
CCGCAGG	65	4.6316563E-7	26.5	23
GATCCAA	65	4.6316563E-7	26.5	16
>>END_MODULE
Read 33528 spots for SRR23332606.sra
Written 33528 spots for SRR23332606.sra
Read 33528 spots for SRR23332606.sra
Written 33528 spots for SRR23332606.sra
Read 33528 spots for SRR23332606.sra
Written 33528 spots for SRR23332606.sra
Read 33528 spots for SRR23332606.sra
Written 33528 spots for SRR23332606.sra
Read 33528 spots for SRR23332606.sra
Written 33528 spots for SRR23332606.sra
Read 33528 spots for SRR23332606.sra
Written 33528 spots for SRR23332606.sra
Read 33528 spots for SRR23332606.sra
Written 33528 spots for SRR23332606.sra
Read 33528 spots for SRR23332606.sra
Written 33528 spots for SRR23332606.sra
SRR ids: ['SRR23332606.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_n0znzwi7
SRR23332606.sra spots: 268224
blocks: [[1, 33528], [33529, 67056], [67057, 100584], [100585, 134112], [134113, 167640], [167641, 201168], [201169, 234696], [234697, 268224]]
SRR23332606 file size 32004
SRR23332606 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR23332606 SRR23332606_1.fastq SRR23332606_2.fastq
Input file:	SRR23332606_1.fastq
Paired file:	SRR23332606_2.fastq
trimmed:	SRR23332606-trimmed-pair1.fastq, SRR23332606-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 06:06:12 2026 >> started

Wed Mar 25 06:06:12 2026 >> done (0.237s)
268224 read pairs processed; of these:
    16 ( 0.01%) short read pairs filtered out after trimming by size control
     0 ( 0.00%) empty read pairs filtered out after trimming by size control
268208 (99.99%) read pairs available; of these:
  1673 ( 0.62%) trimmed read pairs available after processing
266535 (99.38%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     7	  0.00%
 19	    13	  0.00%
 20	    30	  0.01%
 21	    28	  0.01%
 22	     8	  0.00%
 23	    12	  0.00%
 24	    13	  0.00%
 25	    32	  0.01%
 26	    27	  0.01%
 27	    34	  0.01%
 28	    16	  0.01%
 29	    32	  0.01%
 30	    19	  0.01%
 31	    40	  0.01%
 32	    32	  0.01%
 33	    50	  0.02%
 34	    78	  0.03%
 35	    99	  0.04%
 36	   109	  0.04%
 37	    75	  0.03%
 38	    97	  0.04%
 39	   115	  0.04%
 40	   296	  0.11%
 41	   325	  0.12%
 42	  9588	  3.57%
 43	  5796	  2.16%
 44	 23741	  8.85%
 45	227496	 84.82%
268208 reads passed initial QC


criterion=sequence-density
sequence-density=1.10
sequence-density-rank=1
fanout-score=2.47
fanout-score-rank=13
prefix-density=1.12
prefix-fanout=2.4
sequence=ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG


criterion=fanout-score
sequence-density=0.10
sequence-density-rank=28
fanout-score=6.48
fanout-score-rank=1
prefix-density=0.64
prefix-fanout=1.0
sequence=TAGCGATTCCGACTTCATG


criterion=sequence-density
sequence-density=19.05
sequence-density-rank=1
fanout-score=15.76
fanout-score-rank=4
prefix-density=16.36
prefix-fanout=15.8
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTC


criterion=fanout-score
sequence-density=0.63
sequence-density-rank=15
fanout-score=114.63
fanout-score-rank=1
prefix-density=7.25
prefix-fanout=9.9
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTACTCGGTAC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG -y GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTC -o SRR23332606 SRR23332606_1.fastq SRR23332606_2.fastq
Input file:	SRR23332606_1.fastq
Paired file:	SRR23332606_2.fastq
trimmed:	SRR23332606-trimmed-pair1.fastq, SRR23332606-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG
-- paired 3' end adapter sequence (-y):	GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 06:06:14 2026 >> started

Wed Mar 25 06:06:14 2026 >> done (0.219s)
219443 read pairs processed; of these:
  2183 ( 0.99%) short read pairs filtered out after trimming by size control
    75 ( 0.03%) empty read pairs filtered out after trimming by size control
217185 (98.97%) read pairs available; of these:
    50 ( 0.02%) trimmed read pairs available after processing
217135 (99.98%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     6	  0.00%
 19	    10	  0.00%
 20	    30	  0.01%
 21	    23	  0.01%
 22	     8	  0.00%
 23	    10	  0.00%
 24	    11	  0.01%
 25	    24	  0.01%
 26	    22	  0.01%
 27	    27	  0.01%
 28	    11	  0.01%
 29	    24	  0.01%
 30	    16	  0.01%
 31	    30	  0.01%
 32	    27	  0.01%
 33	    37	  0.02%
 34	    62	  0.03%
 35	    87	  0.04%
 36	    79	  0.04%
 37	    63	  0.03%
 38	    80	  0.04%
 39	    92	  0.04%
 40	   240	  0.11%
 41	   272	  0.13%
 42	  7786	  3.58%
 43	  4725	  2.18%
 44	 18807	  8.66%
 45	184576	 84.99%


criterion=sequence-density
sequence-density=1.09
sequence-density-rank=1
fanout-score=1.94
fanout-score-rank=26
prefix-density=0.94
prefix-fanout=1.9
sequence=GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGA


criterion=fanout-score
sequence-density=0.10
sequence-density-rank=28
fanout-score=6.48
fanout-score-rank=1
prefix-density=0.65
prefix-fanout=1.0
sequence=TAGCGATTCCGACTTCATG


criterion=sequence-density
sequence-density=18.46
sequence-density-rank=1
fanout-score=15.46
fanout-score-rank=4
prefix-density=16.11
prefix-fanout=15.5
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTC


criterion=fanout-score
sequence-density=0.63
sequence-density-rank=15
fanout-score=114.63
fanout-score-rank=1
prefix-density=7.31
prefix-fanout=9.9
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTACTCGGTAC
SRR23332606 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 25 06:06:27
                             Started mapping on |	Mar 25 06:06:27
                                    Finished on |	Mar 25 06:07:08
       Mapping speed, Million of reads per hour |	23.35

                          Number of input reads |	265950
                      Average input read length |	90
                                    UNIQUE READS:
                   Uniquely mapped reads number |	164169
                        Uniquely mapped reads % |	61.73%
                          Average mapped length |	87.74
                       Number of splices: Total |	7092
            Number of splices: Annotated (sjdb) |	14
                       Number of splices: GT/AG |	6455
                       Number of splices: GC/AG |	162
                       Number of splices: AT/AC |	16
               Number of splices: Non-canonical |	459
                      Mismatch rate per base, % |	0.26%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.34
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	46215
             % of reads mapped to multiple loci |	17.38%
        Number of reads mapped to too many loci |	42109
             % of reads mapped to too many loci |	15.83%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.68%
                     % of reads unmapped: other |	0.38%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	55566	55566	55566
N_multimapping	46215	46215	46215
N_noFeature	4912	151699	10150
N_ambiguous	13309	1768	4872
UnstrandedReadsAssigned:145948 PositiveStrandReadsAssigned:10702 NegativeStrandReadsAssigned:149147
Dataset is classified negative stranded
MeadianReadLen=41 20thPercentileLength=41 echo kmer=37
SRR23332606 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR23332606-trimmed-pair1.fastq
                             SRR23332606-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 265,950 reads, 56,251 reads pseudoaligned
[quant] estimated average fragment length: 194.196
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 72 rounds

  4323 SRR23332606.ke.tsv
  4497 SRR23332606.se.tsv
  8820 total
==> SRR23332606.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	10.9745	5	1436.39
AAC73113	2463	2269.8	10	13.8899
AAC73114	933	739.804	1	4.26158
AAC73115	1287	1093.8	11	31.7059
AAC73116	297	125.636	3	75.2827
AAC73117	777	583.859	2	10.7996
AAC73118	1431	1237.8	1	2.54704
AAC73119	954	760.804	36	149.182
AAC73120	588	395.867	1	7.96413

==> SRR23332606.se.tsv <==
b0001	21
b0002	9
b0003	1
b0004	11
b0005	3
b0006	3
b0007	1
b0008	36
b0009	1
b0010	0
SRR23332606 completed mapping pipeline successfully
