Starting /dee2/code/volunteer_pipeline.sh SRR23332585
    current disk space = 1439536164
    free memory = 57231000 
SRR23332585 SRAfilesize
39d09266fb750013eb95e8be4aea2aa8  SRR23332585.sra
SRR23332585.sra file validated
SRR23332585 is paired end
SRR23332585 is conventional basespace
SRR23332585 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332585_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.5945	37.0	37.0	37.0	37.0	37.0
2	36.498	37.0	37.0	37.0	37.0	37.0
3	36.444	37.0	37.0	37.0	37.0	37.0
4	36.3875	37.0	37.0	37.0	37.0	37.0
5	36.3865	37.0	37.0	37.0	37.0	37.0
6	36.326	37.0	37.0	37.0	37.0	37.0
7	36.374	37.0	37.0	37.0	37.0	37.0
8	36.4255	37.0	37.0	37.0	37.0	37.0
9	36.492	37.0	37.0	37.0	37.0	37.0
10	36.4585	37.0	37.0	37.0	37.0	37.0
11	36.46	37.0	37.0	37.0	37.0	37.0
12	36.4235	37.0	37.0	37.0	37.0	37.0
13	36.4555	37.0	37.0	37.0	37.0	37.0
14	36.4675	37.0	37.0	37.0	37.0	37.0
15	36.515	37.0	37.0	37.0	37.0	37.0
16	36.3235	37.0	37.0	37.0	37.0	37.0
17	36.339	37.0	37.0	37.0	37.0	37.0
18	36.405	37.0	37.0	37.0	37.0	37.0
19	36.3185	37.0	37.0	37.0	37.0	37.0
20	36.2865	37.0	37.0	37.0	37.0	37.0
21	36.445	37.0	37.0	37.0	37.0	37.0
22	36.371	37.0	37.0	37.0	37.0	37.0
23	36.384	37.0	37.0	37.0	37.0	37.0
24	36.3635	37.0	37.0	37.0	37.0	37.0
25	36.396	37.0	37.0	37.0	37.0	37.0
26	36.3135	37.0	37.0	37.0	37.0	37.0
27	36.3245	37.0	37.0	37.0	37.0	37.0
28	36.3915	37.0	37.0	37.0	37.0	37.0
29	36.328	37.0	37.0	37.0	37.0	37.0
30	36.4	37.0	37.0	37.0	37.0	37.0
31	36.4125	37.0	37.0	37.0	37.0	37.0
32	36.3935	37.0	37.0	37.0	37.0	37.0
33	36.3695	37.0	37.0	37.0	37.0	37.0
34	36.309	37.0	37.0	37.0	37.0	37.0
35	36.3265	37.0	37.0	37.0	37.0	37.0
36	36.2195	37.0	37.0	37.0	37.0	37.0
37	36.273	37.0	37.0	37.0	37.0	37.0
38	36.3005	37.0	37.0	37.0	37.0	37.0
39	36.1675	37.0	37.0	37.0	37.0	37.0
40	36.287	37.0	37.0	37.0	37.0	37.0
41	36.349	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	2.0
26	7.0
27	13.0
28	15.0
29	15.0
30	33.0
31	33.0
32	40.0
33	69.0
34	107.0
35	214.0
36	1402.0
37	2050.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.25	19.925	46.9	9.925
2	32.125	17.925	29.275000000000002	20.674999999999997
3	33.925	21.25	26.150000000000002	18.675
4	19.725	24.65	35.5	20.125
5	33.175	24.6	22.3	19.925
6	34.075	23.599999999999998	21.25	21.075
7	21.45	36.425000000000004	19.5	22.625
8	37.275000000000006	18.775	18.95	25.0
9	25.5	15.35	20.875	38.275
10	26.325	15.4	31.825	26.450000000000003
11	37.625	16.475	19.15	26.75
12	38.6	17.025000000000002	21.15	23.225
13	25.45	18.075	18.775	37.7
14	37.375	21.349999999999998	20.25	21.025
15	39.675	20.424999999999997	20.5	19.400000000000002
16	39.4	22.275	20.424999999999997	17.9
17	25.074999999999996	35.725	20.75	18.45
18	36.55	20.225	23.325000000000003	19.900000000000002
19	23.75	19.1	36.9	20.25
20	25.724999999999998	19.400000000000002	34.625	20.25
21	38.574999999999996	21.3	17.525	22.6
22	24.075	34.300000000000004	20.375	21.25
23	24.775	33.7	19.400000000000002	22.125
24	24.2	19.875	18.575	37.35
25	23.225	19.925	20.025000000000002	36.825
26	24.9	17.8	21.775	35.525
27	38.550000000000004	19.15	19.575	22.725
28	24.25	18.925	20.849999999999998	35.975
29	37.75	17.675	21.45	23.125
30	21.375	18.825	38.824999999999996	20.974999999999998
31	21.275	20.375	23.025000000000002	35.325
32	23.125	20.875	21.65	34.35
33	37.0	21.725	20.474999999999998	20.8
34	20.625	36.1	20.525	22.75
35	21.925	35.575	21.675	20.825
36	21.575	36.35	21.075	21.0
37	20.974999999999998	21.7	34.300000000000004	23.025000000000002
38	24.3	20.325	33.925	21.45
39	22.0	20.7	20.349999999999998	36.95
40	21.675	21.675	21.099999999999998	35.55
41	21.05	21.325	34.8	22.825
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	1.0
20	1.0
21	0.5
22	0.0
23	1.5
24	3.0
25	3.0
26	3.5
27	4.0
28	7.5
29	11.0
30	15.5
31	20.0
32	20.0
33	25.5
34	31.0
35	38.0
36	45.0
37	45.0
38	63.5
39	82.0
40	119.0
41	156.0
42	156.0
43	194.5
44	233.0
45	278.5
46	324.0
47	324.0
48	366.0
49	408.0
50	443.5
51	479.0
52	483.0
53	487.0
54	487.0
55	451.0
56	415.0
57	355.0
58	295.0
59	295.0
60	552.5
61	810.0
62	456.0
63	102.0
64	102.0
65	76.5
66	51.0
67	39.0
68	27.0
69	27.0
70	17.5
71	8.0
72	6.0
73	4.0
74	3.5
75	3.0
76	3.0
77	2.0
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.88381742738589	46.925
2	15.020746887966805	18.099999999999998
3	3.4439834024896268	6.225
4	1.4522821576763485	3.5000000000000004
5	0.7883817427385892	2.375
6	0.4564315352697096	1.6500000000000001
7	0.2074688796680498	0.8750000000000001
8	0.08298755186721991	0.4
9	0.16597510373443983	0.8999999999999999
>10	0.4564315352697096	5.050000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.04149377593360996	14.000000000000002
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCT	560	14.000000000000002	No Hit
GGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTA	36	0.8999999999999999	No Hit
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTAC	28	0.7000000000000001	No Hit
AAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGAG	28	0.7000000000000001	No Hit
GGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAAC	17	0.42500000000000004	No Hit
AAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTT	17	0.42500000000000004	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCC	16	0.4	No Hit
AGGAAGTAGGCAGCTGACAGAGATCGGAAGAGCACACGTCT	15	0.375	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACA	13	0.325	No Hit
GGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAA	12	0.3	No Hit
GGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTC	10	0.25	No Hit
TTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACT	10	0.25	No Hit
AAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTC	9	0.22499999999999998	No Hit
GTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTA	9	0.22499999999999998	No Hit
AAAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGA	9	0.22499999999999998	No Hit
TTATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGAA	9	0.22499999999999998	No Hit
AAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGAGG	8	0.2	No Hit
AAAAAACGCGCCCGAAGGCGCGTTGGCGATACACTCAATAT	8	0.2	No Hit
GGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	7	0.17500000000000002	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACGAGAGGACCGGGA	7	0.17500000000000002	No Hit
TGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	7	0.17500000000000002	No Hit
CTTCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAA	7	0.17500000000000002	No Hit
TTCTGATCCACGGTGAATACGTTCCCGGGCCTTGTACACAC	7	0.17500000000000002	No Hit
TCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTCCTG	6	0.15	No Hit
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAGTTCCT	6	0.15	No Hit
AAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGT	6	0.15	No Hit
AAAAAAATGGCGCACAATGTGCGCCATTTTTCACTTCACAG	6	0.15	No Hit
AGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAA	6	0.15	No Hit
TAAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGAA	6	0.15	No Hit
AGGTTAAGCCTCACGGTTCATTAGTACGAGAGGACCGGGAT	6	0.15	No Hit
AAAATGCGCCACCGTGTTAGGGTGGCGCGTGCCCTGCTTTT	6	0.15	No Hit
TCTCGTACTAGGAGTGGACGCATCACTGGTGTTCGGGTTGT	6	0.15	No Hit
GTTGCGGGACTTAACCTTCGGGAGGGCGCTTACCACTTTGT	6	0.15	No Hit
TTCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTCCT	6	0.15	No Hit
TGAGGTCCGCTTGGAGTCTGCAACTCGACTCCATGAAGTCG	5	0.125	No Hit
TCCACGCGATCTCTTTTGGGTTTGACCTCTCTTGATCCCCG	5	0.125	No Hit
AAGCACCATCATTTACTAAAACAAGTAAATAAACGAGAGAT	5	0.125	No Hit
GGCAGGGCTTCCACGCGATCTCTTTTGGGTTTGACCTCTCT	5	0.125	No Hit
GGTGTGACGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGA	5	0.125	No Hit
ACAGTGATCCCTGTGAATATTACATCTGCTATACCGATTAA	5	0.125	No Hit
AGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGT	5	0.125	No Hit
TTTATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGA	5	0.125	No Hit
TACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACTGG	5	0.125	No Hit
ATTCACCGTGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTA	5	0.125	No Hit
TCCTACATCCTCGGTACTACATGCTTAGTCAGTCTTTACAT	5	0.125	No Hit
AAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCG	5	0.125	No Hit
TCTCAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGT	5	0.125	No Hit
TTACATTCGCTTGCCAGCTGCGGACGGACACGCCACTAACA	5	0.125	No Hit
TATCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGA	5	0.125	No Hit
AAGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGA	5	0.125	No Hit
AAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGC	5	0.125	No Hit
TGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACA	5	0.125	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.05	0.0	0.0	0.0	0.0
12	0.05	0.0	0.0	0.0	0.0
13	0.05	0.0	0.0	0.0	0.0
14	0.05	0.0	0.0	0.0	0.0
15	0.05	0.0	0.0	0.0	0.0
16	0.05	0.0	0.0	0.0	0.0
17	0.05	0.0	0.0	0.0	0.0
18	0.075	0.0	0.0	0.0	0.0
19	0.075	0.0	0.0	0.0	0.0
20	0.125	0.0	0.0	0.0	0.0
21	0.55	0.0	0.0	0.0	0.0
22	0.6	0.0	0.0	0.0	0.0
23	0.6	0.0	0.0	0.0	0.0
24	0.625	0.0	0.0	0.0	0.0
25	0.625	0.0	0.0	0.0	0.0
26	0.65	0.0	0.0	0.0	0.0
27	0.675	0.0	0.0	0.0	0.0
28	0.7	0.0	0.0	0.0	0.0
29	0.75	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTGGA	60	0.0	35.000004	1
GGTGGAG	70	5.456968E-12	30.000002	2
GTGGAGC	70	5.456968E-12	30.000002	3
GCGGGAG	75	1.0913936E-11	28.0	12
GTTGAAC	75	1.0913936E-11	28.0	18
GAAATTC	75	1.0913936E-11	28.0	33
AAATTCC	75	1.0913936E-11	28.0	34
AATTCCT	75	1.0913936E-11	28.0	35
GTCCGAA	75	1.0913936E-11	28.0	29
GCTGGCG	75	1.0913936E-11	28.0	8
GAGCTGG	75	1.0913936E-11	28.0	6
AACCCGC	75	1.0913936E-11	28.0	22
ACCCGCG	75	1.0913936E-11	28.0	23
TGGAGCT	75	1.0913936E-11	28.0	4
GGGAGTT	75	1.0913936E-11	28.0	14
GAGTTGA	75	1.0913936E-11	28.0	16
CCCGCGT	75	1.0913936E-11	28.0	24
GAACCCG	75	1.0913936E-11	28.0	21
CGGGAGT	75	1.0913936E-11	28.0	13
GCGTCCG	75	1.0913936E-11	28.0	27
>>END_MODULE
SRR23332585 read2 length is 41-50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332585_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41-50
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	19.2845	11.0	11.0	25.0	11.0	37.0
2	22.018	11.0	11.0	37.0	11.0	37.0
3	25.0295	25.0	11.0	37.0	11.0	37.0
4	35.627	37.0	37.0	37.0	37.0	37.0
5	35.947	37.0	37.0	37.0	37.0	37.0
6	35.8345	37.0	37.0	37.0	37.0	37.0
7	35.839	37.0	37.0	37.0	37.0	37.0
8	35.9845	37.0	37.0	37.0	37.0	37.0
9	35.803	37.0	37.0	37.0	37.0	37.0
10	35.8535	37.0	37.0	37.0	37.0	37.0
11	35.869	37.0	37.0	37.0	37.0	37.0
12	35.948	37.0	37.0	37.0	37.0	37.0
13	36.05	37.0	37.0	37.0	37.0	37.0
14	36.0895	37.0	37.0	37.0	37.0	37.0
15	35.8125	37.0	37.0	37.0	37.0	37.0
16	36.055	37.0	37.0	37.0	37.0	37.0
17	36.051	37.0	37.0	37.0	37.0	37.0
18	36.1005	37.0	37.0	37.0	37.0	37.0
19	36.0635	37.0	37.0	37.0	37.0	37.0
20	35.993	37.0	37.0	37.0	37.0	37.0
21	35.88525	37.0	37.0	37.0	37.0	37.0
22	36.078	37.0	37.0	37.0	37.0	37.0
23	36.056	37.0	37.0	37.0	37.0	37.0
24	36.161	37.0	37.0	37.0	37.0	37.0
25	36.048	37.0	37.0	37.0	37.0	37.0
26	36.1695	37.0	37.0	37.0	37.0	37.0
27	35.99575	37.0	37.0	37.0	37.0	37.0
28	36.06825	37.0	37.0	37.0	37.0	37.0
29	35.9785	37.0	37.0	37.0	37.0	37.0
30	36.02675	37.0	37.0	37.0	37.0	37.0
31	36.0905	37.0	37.0	37.0	37.0	37.0
32	35.82275	37.0	37.0	37.0	37.0	37.0
33	35.8495	37.0	37.0	37.0	37.0	37.0
34	35.99675	37.0	37.0	37.0	37.0	37.0
35	36.116	37.0	37.0	37.0	37.0	37.0
36	36.20425	37.0	37.0	37.0	37.0	37.0
37	36.067	37.0	37.0	37.0	37.0	37.0
38	36.096	37.0	37.0	37.0	37.0	37.0
39	36.0465	37.0	37.0	37.0	37.0	37.0
40	36.011	37.0	37.0	37.0	37.0	37.0
41	35.875	37.0	37.0	37.0	37.0	37.0
42	35.90545272636318	37.0	37.0	37.0	37.0	37.0
43	35.74142678347935	37.0	37.0	37.0	37.0	37.0
44	35.912024199647085	37.0	37.0	37.0	37.0	37.0
45	35.81540847983454	37.0	37.0	37.0	37.0	37.0
46	36.04060385216033	37.0	37.0	37.0	37.0	37.0
47	35.96813273637082	37.0	37.0	37.0	37.0	37.0
48	36.06082337317397	37.0	37.0	37.0	37.0	37.0
49	35.92481417953116	37.0	37.0	37.0	37.0	37.0
50	35.7160669754186	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	1.0
20	4.0
21	3.0
22	6.0
23	10.0
24	12.0
25	12.0
26	15.0
27	25.0
28	26.0
29	38.0
30	44.0
31	53.0
32	82.0
33	147.0
34	478.0
35	1835.0
36	1066.0
37	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	39.425	25.2	35.375
2	25.5	35.025	15.65	23.825
3	29.725	40.775	10.549999999999999	18.95
4	81.575	6.4	5.2	6.825
5	67.30000000000001	14.05	10.325	8.325000000000001
6	56.525000000000006	21.325	10.125	12.025
7	39.35	37.1	10.375	13.175
8	39.900000000000006	21.4	13.225000000000001	25.474999999999998
9	20.9	38.15	27.3	13.65
10	42.15	24.925	15.125	17.8
11	28.425	41.699999999999996	15.5	14.374999999999998
12	23.200000000000003	26.6	26.55	23.65
13	38.574999999999996	13.225000000000001	29.325000000000003	18.875
14	34.425	16.425	24.6	24.55
15	28.525	15.875	43.575	12.025
16	46.725	19.925	22.25	11.1
17	28.225	33.95	17.175	20.65
18	16.55	37.075	28.575	17.8
19	17.8	17.45	36.275	28.475
20	14.95	23.150000000000002	33.324999999999996	28.575
21	19.02377972465582	40.85106382978723	12.991239048811012	27.133917396745932
22	37.925	29.525000000000002	15.375	17.175
23	20.474999999999998	32.45	18.25	28.825
24	22.675	22.45	12.925	41.949999999999996
25	41.9	17.175	11.799999999999999	29.125
26	34.125	21.8	13.025	31.05
27	39.94993742177722	26.958698372966204	18.923654568210264	14.167709637046308
28	35.76971214017522	25.782227784730914	24.80600750938673	13.642052565707132
29	24.9	15.725	44.9	14.475
30	18.29787234042553	15.869837296620776	52.816020025031285	13.016270337922403
31	19.2	21.224999999999998	39.75	19.825
32	33.76720901126408	14.918648310387987	17.922403003754695	33.39173967459324
33	20.36018009004502	13.931965982991496	15.832916458229116	49.874937468734366
34	26.045579764588027	17.981467568244426	22.414224893563738	33.558727773603806
35	14.274999999999999	34.55	22.35	28.825
36	14.367959949937422	40.550688360450565	27.30913642052566	17.77221526908636
37	16.075	47.375	11.85	24.7
38	22.25	17.549999999999997	11.700000000000001	48.5
39	35.699999999999996	16.35	13.200000000000001	34.75
40	23.9	21.55	18.5	36.05
41	17.45	40.300000000000004	28.499999999999996	13.750000000000002
42	20.785392696348172	31.840920460230116	28.489244622311155	18.884442221110557
43	33.04130162703379	24.6558197747184	15.74468085106383	26.558197747183982
44	36.80362994706327	14.923115704562642	19.964708847995965	28.30854550037812
45	17.580144777662877	15.434332988624613	34.4364012409514	32.54912099276112
46	30.86933888599688	17.230609057782406	32.196772514315455	19.703279541905257
47	27.758756913352645	16.038978140637344	26.415591256254938	29.786673689755073
48	27.99468791500664	26.905710491367863	24.780876494023904	20.318725099601593
49	27.16862296020613	12.854279988548525	42.399083882049815	17.578013169195533
50	31.635197719985754	14.35696473102957	29.533309583184895	24.474527965799787
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	1.0
25	2.0
26	3.0
27	2.5
28	2.5
29	7.0
30	11.5
31	18.5
32	28.0
33	37.5
34	46.0
35	42.5
36	38.5
37	46.5
38	57.0
39	77.5
40	98.0
41	103.0
42	103.0
43	116.5
44	135.0
45	151.5
46	170.0
47	183.0
48	187.0
49	194.5
50	221.0
51	361.5
52	495.5
53	637.5
54	802.0
55	811.0
56	770.5
57	658.5
58	518.0
59	346.0
60	192.5
61	118.0
62	66.0
63	50.5
64	39.0
65	34.0
66	27.0
67	19.5
68	13.0
69	8.5
70	5.5
71	6.0
72	5.5
73	3.0
74	2.5
75	2.5
76	1.5
77	1.0
78	1.0
79	1.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	1.0
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.125
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.125
28	0.125
29	0.0
30	0.125
31	0.0
32	0.125
33	0.05
34	0.17500000000000002
35	0.0
36	0.125
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.1429388221841052
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
41	2.0
42	3.0
43	28.0
44	99.0
45	26.0
46	45.0
47	32.0
48	267.0
49	691.0
50	2807.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.41344195519348	53.65
2	3.8696537678207736	4.75
3	2.158859470468432	3.975
4	1.7515274949083504	4.3
5	0.8961303462321792	2.75
6	0.8146639511201629	3.0
7	0.4480651731160896	1.925
8	0.4480651731160896	2.1999999999999997
9	0.1629327902240326	0.8999999999999999
>10	1.9551934826883912	18.825
>50	0.0814663951120163	3.7249999999999996
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	81	2.025	No Hit
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	68	1.7000000000000002	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	34	0.8500000000000001	No Hit
AAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	34	0.8500000000000001	No Hit
ACAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	32	0.8	No Hit
AAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	25	0.625	No Hit
CAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	24	0.6	No Hit
CCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	24	0.6	No Hit
CGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	23	0.575	No Hit
ATAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	22	0.5499999999999999	No Hit
AACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	21	0.525	No Hit
CCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	20	0.5	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	19	0.475	No Hit
TAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	18	0.44999999999999996	No Hit
CCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	17	0.42500000000000004	No Hit
CTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
CGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	16	0.4	No Hit
CGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
CACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
CGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
CAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
AGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	15	0.375	No Hit
TTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	14	0.35000000000000003	No Hit
CGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
CAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	14	0.35000000000000003	No Hit
CCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
AATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
TGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
ATAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
CCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
CAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	13	0.325	No Hit
CTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
AAAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	12	0.3	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	12	0.3	No Hit
AAAGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	12	0.3	No Hit
CCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
ACAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
ACAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	12	0.3	No Hit
CGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	12	0.3	No Hit
TCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
AATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
ACCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
ACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
TGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
TAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
CGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
TGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
TCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
AATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
CCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
AGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
TACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
TAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
CTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
AAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
AAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
TCCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
TTCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TCGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CCCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
ATAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
ATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TTGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
AAAGGGAGAGTCCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCA	6	0.15	No Hit
TGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
ACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
AGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
ACCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
AGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
ATAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CTGTCAGCTGCCTACTTCCTAAATATTCAAGATCGGAAGAGCGTCGTGTA	6	0.15	Illumina Single End PCR Primer 1 (100% over 21bp)
TCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AACGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TTGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTA	5	0.125	No Hit
ATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TTCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
AAACGAATGGCGTAATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGA	5	0.125	No Hit
CACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TGCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CTGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
AGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CTCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGT	5	0.125	No Hit
ACTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CCCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TTTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.025	0.0	0.0	0.0	0.0
18	0.025	0.0	0.0	0.0	0.0
19	0.05	0.0	0.0	0.0	0.0
20	0.05	0.0	0.0	0.0	0.0
21	0.05	0.0	0.0	0.0	0.0
22	0.075	0.0	0.0	0.0	0.0
23	0.1	0.0	0.0	0.0	0.0
24	0.1	0.0	0.0	0.0	0.0
25	0.1	0.0	0.0	0.0	0.0
26	0.1	0.0	0.0	0.0	0.0
27	0.125	0.0	0.0	0.0	0.0
28	0.125	0.0	0.0	0.0	0.0
29	0.15	0.0	0.0	0.0	0.0
30	0.575	0.0	0.0	0.0	0.0
31	0.625	0.0	0.0	0.0	0.0
32	0.625	0.0	0.0	0.0	0.0
33	0.7	0.0	0.0	0.0	0.0
34	0.725	0.0	0.0	0.0	0.0
35	0.75	0.0	0.0	0.0	0.0
36	0.75	0.0	0.0	0.0	0.0
37	0.75	0.0	0.0	0.0	0.0
38	0.775	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTGCGC	30	9.587129E-6	54.355343	44
GTAGTGC	30	9.587129E-6	54.355343	44
CGCTGCG	30	3.7216865E-5	43.64899	43
CGTAGTG	30	3.7216865E-5	43.64899	43
AAGGGAG	35	1.17905074E-7	43.2125	2
AAAGGGA	30	2.210143E-6	43.2125	1
GTGCATG	70	3.237801E-10	41.931263	44
AGGGAGA	70	0.0	40.125893	3
ACCGTAG	30	6.900701E-5	39.46347	41
CCGTAGT	30	6.900701E-5	39.46347	42
ATCGCTG	30	6.900701E-5	39.46347	41
CCTTGTT	75	6.384653E-10	39.13585	44
GGAGACA	45	1.7673301E-8	38.41111	5
GGGAGAC	45	1.7673301E-8	38.41111	4
CACCGTA	30	8.822835E-5	37.905704	40
GCACCGT	30	8.822835E-5	37.905704	39
GCATCGC	30	8.822835E-5	37.905704	39
CATCGCT	30	8.822835E-5	37.905704	40
ACACGGT	40	3.368441E-7	37.81094	9
GACACGG	40	3.368441E-7	37.81094	8
>>END_MODULE
Read 265928 spots for SRR23332585.sra
Written 265928 spots for SRR23332585.sra
Read 265924 spots for SRR23332585.sra
Written 265924 spots for SRR23332585.sra
Read 265924 spots for SRR23332585.sra
Written 265924 spots for SRR23332585.sra
Read 265924 spots for SRR23332585.sra
Written 265924 spots for SRR23332585.sra
Read 265924 spots for SRR23332585.sra
Written 265924 spots for SRR23332585.sra
Read 265924 spots for SRR23332585.sra
Written 265924 spots for SRR23332585.sra
Read 265924 spots for SRR23332585.sra
Written 265924 spots for SRR23332585.sra
Read 265924 spots for SRR23332585.sra
Written 265924 spots for SRR23332585.sra
SRR ids: ['SRR23332585.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_4unx_8t5
SRR23332585.sra spots: 2127396
blocks: [[1, 265924], [265925, 531848], [531849, 797772], [797773, 1063696], [1063697, 1329620], [1329621, 1595544], [1595545, 1861468], [1861469, 2127396]]
SRR23332585 file size 257524
SRR23332585 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR23332585 SRR23332585_1.fastq SRR23332585_2.fastq
Input file:	SRR23332585_1.fastq
Paired file:	SRR23332585_2.fastq
trimmed:	SRR23332585-trimmed-pair1.fastq, SRR23332585-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 06:59:10 2026 >> started

Wed Mar 25 06:59:12 2026 >> done (1.755s)
2127396 read pairs processed; of these:
    374 ( 0.02%) short read pairs filtered out after trimming by size control
      0 ( 0.00%) empty read pairs filtered out after trimming by size control
2127022 (99.98%) read pairs available; of these:
  20032 ( 0.94%) trimmed read pairs available after processing
2106990 (99.06%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    142	  0.01%
 19	    444	  0.02%
 20	   8981	  0.42%
 21	     89	  0.00%
 22	    115	  0.01%
 23	    173	  0.01%
 24	    150	  0.01%
 25	    164	  0.01%
 26	    145	  0.01%
 27	    236	  0.01%
 28	    187	  0.01%
 29	    395	  0.02%
 30	    246	  0.01%
 31	    312	  0.01%
 32	    320	  0.02%
 33	    394	  0.02%
 34	    602	  0.03%
 35	    632	  0.03%
 36	    752	  0.04%
 37	    645	  0.03%
 38	    733	  0.03%
 39	    838	  0.04%
 40	   2086	  0.10%
 41	   2028	  0.10%
 42	  75926	  3.57%
 43	  43432	  2.04%
 44	 198075	  9.31%
 45	1788780	 84.10%
2127022 reads passed initial QC


criterion=sequence-density
sequence-density=1.43
sequence-density-rank=1
fanout-score=2.49
fanout-score-rank=13
prefix-density=1.37
prefix-fanout=2.5
sequence=ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=34
fanout-score=7.43
fanout-score-rank=1
prefix-density=0.09
prefix-fanout=1.9
sequence=GAACCCTTGGTCTTCCGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTAC


criterion=sequence-density
sequence-density=16.26
sequence-density-rank=1
fanout-score=19.36
fanout-score-rank=7
prefix-density=13.51
prefix-fanout=19.4
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACC


criterion=fanout-score
sequence-density=0.56
sequence-density-rank=12
fanout-score=187.72
fanout-score-rank=1
prefix-density=9.24
prefix-fanout=11.4
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTAAATATTCA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG -y GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACC -o SRR23332585 SRR23332585_1.fastq SRR23332585_2.fastq
Input file:	SRR23332585_1.fastq
Paired file:	SRR23332585_2.fastq
trimmed:	SRR23332585-trimmed-pair1.fastq, SRR23332585-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG
-- paired 3' end adapter sequence (-y):	GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 06:59:18 2026 >> started

Wed Mar 25 06:59:19 2026 >> done (1.550s)
1701618 read pairs processed; of these:
  17611 ( 1.03%) short read pairs filtered out after trimming by size control
   1066 ( 0.06%) empty read pairs filtered out after trimming by size control
1682941 (98.90%) read pairs available; of these:
    426 ( 0.03%) trimmed read pairs available after processing
1682515 (99.97%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    108	  0.01%
 19	    368	  0.02%
 20	   7141	  0.42%
 21	     78	  0.00%
 22	     94	  0.01%
 23	    145	  0.01%
 24	    120	  0.01%
 25	    132	  0.01%
 26	    121	  0.01%
 27	    187	  0.01%
 28	    159	  0.01%
 29	    314	  0.02%
 30	    195	  0.01%
 31	    247	  0.01%
 32	    260	  0.02%
 33	    311	  0.02%
 34	    486	  0.03%
 35	    506	  0.03%
 36	    606	  0.04%
 37	    503	  0.03%
 38	    587	  0.03%
 39	    681	  0.04%
 40	   1682	  0.10%
 41	   1614	  0.10%
 42	  60292	  3.58%
 43	  34716	  2.06%
 44	 153079	  9.10%
 45	1418209	 84.27%


criterion=sequence-density
sequence-density=1.38
sequence-density-rank=1
fanout-score=2.49
fanout-score-rank=13
prefix-density=1.39
prefix-fanout=2.5
sequence=ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=34
fanout-score=7.31
fanout-score-rank=1
prefix-density=0.09
prefix-fanout=1.9
sequence=GAACCCTTGGTCTTCCGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTAC


criterion=sequence-density
sequence-density=15.60
sequence-density-rank=1
fanout-score=19.00
fanout-score-rank=7
prefix-density=13.25
prefix-fanout=19.0
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACC


criterion=fanout-score
sequence-density=0.57
sequence-density-rank=12
fanout-score=187.03
fanout-score-rank=1
prefix-density=9.33
prefix-fanout=11.4
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTAAATATTCA
SRR23332585 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 25 06:59:35
                             Started mapping on |	Mar 25 06:59:35
                                    Finished on |	Mar 25 07:01:15
       Mapping speed, Million of reads per hour |	75.90

                          Number of input reads |	2108345
                      Average input read length |	90
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1251502
                        Uniquely mapped reads % |	59.36%
                          Average mapped length |	87.84
                       Number of splices: Total |	64896
            Number of splices: Annotated (sjdb) |	744
                       Number of splices: GT/AG |	58540
                       Number of splices: GC/AG |	1308
                       Number of splices: AT/AC |	224
               Number of splices: Non-canonical |	4824
                      Mismatch rate per base, % |	0.27%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.29
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	384184
             % of reads mapped to multiple loci |	18.22%
        Number of reads mapped to too many loci |	352334
             % of reads mapped to too many loci |	16.71%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.24%
                     % of reads unmapped: other |	0.46%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	472659	472659	472659
N_multimapping	384184	384184	384184
N_noFeature	33191	1146076	73593
N_ambiguous	133559	16739	56440
UnstrandedReadsAssigned:1084752 PositiveStrandReadsAssigned:88687 NegativeStrandReadsAssigned:1121469
Dataset is classified negative stranded
MeadianReadLen=41 20thPercentileLength=41 echo kmer=37
SRR23332585 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR23332585-trimmed-pair1.fastq
                             SRR23332585-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,108,345 reads, 482,259 reads pseudoaligned
[quant] estimated average fragment length: 225.632
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 387 rounds

  4323 SRR23332585.ke.tsv
  4497 SRR23332585.se.tsv
  8820 total
==> SRR23332585.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	10.5714	50	2352.28
AAC73113	2463	2238.37	83	18.4417
AAC73114	933	708.368	40	28.0837
AAC73115	1287	1062.37	53	24.8116
AAC73116	297	110.373	8	36.0478
AAC73117	777	552.559	26	23.4017
AAC73118	1431	1206.37	20	8.24524
AAC73119	954	729.368	703	479.36
AAC73120	588	365.811	41	55.7417

==> SRR23332585.se.tsv <==
b0001	76
b0002	76
b0003	33
b0004	50
b0005	8
b0006	28
b0007	19
b0008	702
b0009	52
b0010	5
SRR23332585 completed mapping pipeline successfully
