Starting /dee2/code/volunteer_pipeline.sh SRR23332739
    current disk space = 1463426272
    free memory = 58992504 
SRR23332739 SRAfilesize
ff5f77a533366acfcdf90e83b2f43b34  SRR23332739.sra
SRR23332739.sra file validated
SRR23332739 is paired end
SRR23332739 is conventional basespace
SRR23332739 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332739_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.5205	37.0	37.0	37.0	37.0	37.0
2	36.537	37.0	37.0	37.0	37.0	37.0
3	36.573	37.0	37.0	37.0	37.0	37.0
4	36.416	37.0	37.0	37.0	37.0	37.0
5	36.47	37.0	37.0	37.0	37.0	37.0
6	36.5325	37.0	37.0	37.0	37.0	37.0
7	36.458	37.0	37.0	37.0	37.0	37.0
8	36.4175	37.0	37.0	37.0	37.0	37.0
9	36.4525	37.0	37.0	37.0	37.0	37.0
10	36.473	37.0	37.0	37.0	37.0	37.0
11	36.4995	37.0	37.0	37.0	37.0	37.0
12	36.498	37.0	37.0	37.0	37.0	37.0
13	36.455	37.0	37.0	37.0	37.0	37.0
14	36.4245	37.0	37.0	37.0	37.0	37.0
15	36.5095	37.0	37.0	37.0	37.0	37.0
16	36.5495	37.0	37.0	37.0	37.0	37.0
17	36.37	37.0	37.0	37.0	37.0	37.0
18	36.3905	37.0	37.0	37.0	37.0	37.0
19	36.3855	37.0	37.0	37.0	37.0	37.0
20	36.4265	37.0	37.0	37.0	37.0	37.0
21	36.4285	37.0	37.0	37.0	37.0	37.0
22	36.367	37.0	37.0	37.0	37.0	37.0
23	36.336	37.0	37.0	37.0	37.0	37.0
24	36.4485	37.0	37.0	37.0	37.0	37.0
25	36.371	37.0	37.0	37.0	37.0	37.0
26	36.3375	37.0	37.0	37.0	37.0	37.0
27	36.4835	37.0	37.0	37.0	37.0	37.0
28	36.374	37.0	37.0	37.0	37.0	37.0
29	36.322	37.0	37.0	37.0	37.0	37.0
30	36.41	37.0	37.0	37.0	37.0	37.0
31	36.505	37.0	37.0	37.0	37.0	37.0
32	36.4495	37.0	37.0	37.0	37.0	37.0
33	36.4685	37.0	37.0	37.0	37.0	37.0
34	36.3775	37.0	37.0	37.0	37.0	37.0
35	36.415	37.0	37.0	37.0	37.0	37.0
36	36.2655	37.0	37.0	37.0	37.0	37.0
37	36.443	37.0	37.0	37.0	37.0	37.0
38	36.36	37.0	37.0	37.0	37.0	37.0
39	36.3095	37.0	37.0	37.0	37.0	37.0
40	36.28	37.0	37.0	37.0	37.0	37.0
41	36.289	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	3.0
26	3.0
27	9.0
28	12.0
29	11.0
30	27.0
31	36.0
32	36.0
33	74.0
34	109.0
35	216.0
36	1283.0
37	2180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.650000000000002	15.45	50.575	7.324999999999999
2	26.35	16.400000000000002	34.525	22.725
3	27.575	20.875	30.725	20.825
4	20.75	23.5	34.925	20.825
5	29.875	24.45	23.425	22.25
6	30.5	23.325000000000003	22.7	23.474999999999998
7	21.425	29.975	21.775	26.825
8	30.325000000000003	18.725	23.125	27.825
9	25.05	15.725	23.3	35.925000000000004
10	26.25	18.099999999999998	30.15	25.5
11	31.624999999999996	18.725	20.5	29.15
12	33.550000000000004	19.075	22.175	25.2
13	26.650000000000002	19.025	21.349999999999998	32.975
14	33.85	20.599999999999998	22.35	23.200000000000003
15	35.425000000000004	21.2	21.95	21.425
16	36.35	20.849999999999998	23.225	19.575
17	24.575	29.125	25.275	21.025
18	33.275	21.075	25.7	19.950000000000003
19	26.5	19.525000000000002	34.075	19.900000000000002
20	24.325	22.8	30.175	22.7
21	35.425000000000004	21.425	21.125	22.025
22	24.075	30.925000000000004	21.725	23.275000000000002
23	25.35	30.875000000000004	20.875	22.900000000000002
24	26.650000000000002	19.925	21.075	32.35
25	28.549999999999997	19.55	20.4	31.5
26	25.674999999999997	19.875	22.650000000000002	31.8
27	35.65	20.25	21.5	22.6
28	25.474999999999998	18.875	22.075	33.575
29	35.85	18.6	22.3	23.25
30	23.200000000000003	19.075	34.1	23.625
31	24.125	20.3	24.275	31.3
32	25.224999999999998	22.225	22.375	30.175
33	34.0	21.6	23.875	20.525
34	23.225	31.175000000000004	23.025000000000002	22.575
35	21.975	30.525000000000002	24.65	22.85
36	22.325	31.874999999999996	23.225	22.575
37	25.7	22.5	28.875	22.925
38	25.624999999999996	21.525	29.525000000000002	23.325000000000003
39	24.15	22.875	21.125	31.85
40	23.375	23.875	19.1	33.650000000000006
41	25.0	21.475	28.15	25.374999999999996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	1.0
20	1.0
21	0.5
22	0.0
23	0.0
24	0.0
25	0.0
26	2.0
27	4.0
28	5.5
29	7.0
30	6.5
31	6.0
32	6.0
33	15.5
34	25.0
35	33.5
36	42.0
37	42.0
38	74.0
39	106.0
40	135.5
41	165.0
42	165.0
43	222.0
44	279.0
45	318.0
46	357.0
47	357.0
48	399.0
49	441.0
50	477.5
51	514.0
52	497.0
53	480.0
54	480.0
55	435.5
56	391.0
57	356.0
58	321.0
59	321.0
60	492.5
61	664.0
62	384.0
63	104.0
64	104.0
65	80.0
66	56.0
67	37.5
68	19.0
69	19.0
70	14.5
71	10.0
72	6.0
73	2.0
74	2.5
75	3.0
76	3.0
77	1.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.425000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.42224191404519	40.300000000000004
2	17.634027470093045	19.900000000000002
3	4.918032786885246	8.325000000000001
4	2.4368630926007975	5.5
5	0.7975188303057155	2.25
6	0.7089056269384139	2.4
7	0.48737261852015945	1.925
8	0.5759858218874613	2.6
9	0.2215330084182543	1.125
>10	0.7532122286220646	6.65
>50	0.0	0.0
>100	0.044306601683650866	9.025
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCT	361	9.025	No Hit
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTAC	48	1.2	No Hit
AAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGAG	23	0.575	No Hit
GGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAACC	19	0.475	No Hit
TTTCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTCC	18	0.44999999999999996	No Hit
GGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAAC	18	0.44999999999999996	No Hit
TTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGTCG	15	0.375	No Hit
TTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGA	15	0.375	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCC	13	0.325	No Hit
TTTATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGA	13	0.325	No Hit
GGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTC	11	0.27499999999999997	No Hit
TCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGT	11	0.27499999999999997	No Hit
GTTTCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTC	11	0.27499999999999997	No Hit
AAAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGA	11	0.27499999999999997	No Hit
AAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTC	10	0.25	No Hit
GGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTA	10	0.25	No Hit
TTGCCAGCTGCGGACGGACACGCCACTAACAAACTAGCCTG	10	0.25	No Hit
TTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGTCGT	10	0.25	No Hit
TCCGGATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACG	9	0.22499999999999998	No Hit
TTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGTCGTA	9	0.22499999999999998	No Hit
TTTTTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAG	9	0.22499999999999998	No Hit
TTATCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTG	9	0.22499999999999998	No Hit
TCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGACT	9	0.22499999999999998	No Hit
GCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACTAC	8	0.2	No Hit
TACTTCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTG	8	0.2	No Hit
GAGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGG	8	0.2	No Hit
TTTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAG	8	0.2	No Hit
AAAAAACGCGCCCGAAGGCGCGTTGGCGATACACTCAATAT	8	0.2	No Hit
GACTCTCAAGGAACGTTGAAGACGACGACGTTGATAGGCCG	8	0.2	No Hit
GCGAGGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCAC	8	0.2	No Hit
GGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACAT	8	0.2	No Hit
TTTACATTCGCTTGCCAGCTGCGGACGGACACGCCACTAAC	8	0.2	No Hit
GCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACA	8	0.2	No Hit
TCTCGTACTAGGAGTGGACGCATCACTGGTGTTCGGGTTGT	8	0.2	No Hit
TTCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAG	8	0.2	No Hit
TGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACA	8	0.2	No Hit
TAACAAACTAGCCTGATTAAGTTTTAACGCTTCAACCCCAG	7	0.17500000000000002	No Hit
GCAGGGCTTCCACGCGATCTCTTTTGGGTTTGACCTCTCTT	7	0.17500000000000002	No Hit
TCTGATCCACGGTGAATACGTTCCCGGGCCTTGTACACACC	7	0.17500000000000002	No Hit
GCCACTAACAAACTAGCCTGATTAAGTTTTAACGCTTCAAC	7	0.17500000000000002	No Hit
ACTAACAAACTAGCCTGATTAAGTTTTAACGCTTCAACCCC	7	0.17500000000000002	No Hit
TTTGAGTTCCCGGGCCTTGTACACACCGCCCGTCACACCAT	7	0.17500000000000002	No Hit
CTACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACTG	7	0.17500000000000002	No Hit
GTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTC	7	0.17500000000000002	No Hit
TTCTGATCCACGGTGAATACGTTCCCGGGCCTTGTACACAC	7	0.17500000000000002	No Hit
TTCCTACATCCTCGGTACTACATGCTTAGTCAGTCTTTACA	7	0.17500000000000002	No Hit
TATCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGA	7	0.17500000000000002	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACGAGAGGACCGGGA	6	0.15	No Hit
GAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAACCC	6	0.15	No Hit
GGTTAAGCTACCTACTTTGTGATTCATGACTGGGGTGAAGT	6	0.15	No Hit
TAGCCTGATTAAGTTTTAACGCTTCAACCCCAGGCAGGGCT	6	0.15	No Hit
TTAACGTTGGACAGGGTGAGTCGACCCCTAAGGCGAGGCCG	6	0.15	No Hit
GGTTAAGCTACCTACTTCTTTTGCAACCCACTTTGTGATTC	6	0.15	No Hit
TTGCAGACTCCAATGAAGTCGGAATCGCTAGTAATCGTGGA	6	0.15	No Hit
AGCTACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGA	6	0.15	No Hit
TCAGTCTTTACATTCGCTTGCCAGCTGCGGACGGACACGCC	6	0.15	No Hit
TACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACTGG	6	0.15	No Hit
AGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACG	6	0.15	No Hit
ACTTCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGA	6	0.15	No Hit
CTACTTCTTTTGCAACCCACTTTGTGATTCATGACTGGGGT	6	0.15	No Hit
AGATGCTTTCAGCACTTATCTCTTCCGCATTTAGCTACCGA	6	0.15	No Hit
TTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCAC	6	0.15	No Hit
TTGGGTTTGACCTCTCTTGATCCCCGTCCTAAGAGCGGAGG	6	0.15	No Hit
GACACGCCACTAACAAACTAGCCTGATTAAGTTTTAACGCT	5	0.125	No Hit
ACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACTGGG	5	0.125	No Hit
AAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTT	5	0.125	No Hit
GCGGAGGCTAGGGAGAGAGGGCTCTAAGCAGGTTATTAAGC	5	0.125	No Hit
AGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAA	5	0.125	No Hit
TCTTCCGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGG	5	0.125	No Hit
TCTTCGCGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGC	5	0.125	No Hit
TCTCGCGAGGTCGGAATCGCTAGTAATCGTGGATCAGAATG	5	0.125	No Hit
GATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGC	5	0.125	No Hit
GTCAGGGAGAACGTTGAAGACGACGACGTTGATAGGCCGGG	5	0.125	No Hit
GAACCCTTGGTGTTACTGCGAAGGGGGGACGGAGAAGGCTA	5	0.125	No Hit
TCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGACC	5	0.125	No Hit
TTACTAGCGATTCCCGGGCCTTGTACACACCGCCCGTCACA	5	0.125	No Hit
CTTATCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCT	5	0.125	No Hit
TTATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGAA	5	0.125	No Hit
GTTAAGCTACCTACTTCTTTTGCAACCCACTTTGTGATTCA	5	0.125	No Hit
CCACTAACAAACTAGCCTGATTAAGTTTTAACGCTTCAACC	5	0.125	No Hit
TGCGGACGGACACGCCACTAACAAACTAGCCTGATTAAGTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.125	0.0	0.0	0.0	0.0
17	0.125	0.0	0.0	0.0	0.0
18	0.125	0.0	0.0	0.0	0.0
19	0.125	0.0	0.0	0.0	0.0
20	0.125	0.0	0.0	0.0	0.0
21	0.225	0.0	0.0	0.0	0.0
22	0.25	0.0	0.0	0.0	0.0
23	0.25	0.0	0.0	0.0	0.0
24	0.25	0.0	0.0	0.0	0.0
25	0.25	0.0	0.0	0.0	0.0
26	0.275	0.0	0.0	0.0	0.0
27	0.3	0.0	0.0	0.0	0.0
28	0.3	0.0	0.0	0.0	0.0
29	0.325	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR23332739 read2 length is 40-50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332739_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	40-50
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	19.8355	11.0	11.0	37.0	11.0	37.0
2	22.12725	11.0	11.0	37.0	11.0	37.0
3	24.4815	25.0	11.0	37.0	11.0	37.0
4	36.0555	37.0	37.0	37.0	37.0	37.0
5	36.2875	37.0	37.0	37.0	37.0	37.0
6	36.111	37.0	37.0	37.0	37.0	37.0
7	35.655	37.0	37.0	37.0	37.0	37.0
8	36.1545	37.0	37.0	37.0	37.0	37.0
9	35.685	37.0	37.0	37.0	37.0	37.0
10	36.0755	37.0	37.0	37.0	37.0	37.0
11	36.0095	37.0	37.0	37.0	37.0	37.0
12	35.934	37.0	37.0	37.0	37.0	37.0
13	36.134	37.0	37.0	37.0	37.0	37.0
14	36.051	37.0	37.0	37.0	37.0	37.0
15	35.806	37.0	37.0	37.0	37.0	37.0
16	36.11025	37.0	37.0	37.0	37.0	37.0
17	36.184	37.0	37.0	37.0	37.0	37.0
18	36.261	37.0	37.0	37.0	37.0	37.0
19	36.1795	37.0	37.0	37.0	37.0	37.0
20	36.0785	37.0	37.0	37.0	37.0	37.0
21	36.01875	37.0	37.0	37.0	37.0	37.0
22	36.2065	37.0	37.0	37.0	37.0	37.0
23	36.192	37.0	37.0	37.0	37.0	37.0
24	36.0785	37.0	37.0	37.0	37.0	37.0
25	36.0845	37.0	37.0	37.0	37.0	37.0
26	36.11	37.0	37.0	37.0	37.0	37.0
27	36.13925	37.0	37.0	37.0	37.0	37.0
28	36.07825	37.0	37.0	37.0	37.0	37.0
29	36.1715	37.0	37.0	37.0	37.0	37.0
30	36.07475	37.0	37.0	37.0	37.0	37.0
31	36.0825	37.0	37.0	37.0	37.0	37.0
32	36.05275	37.0	37.0	37.0	37.0	37.0
33	36.191	37.0	37.0	37.0	37.0	37.0
34	36.17525	37.0	37.0	37.0	37.0	37.0
35	36.1165	37.0	37.0	37.0	37.0	37.0
36	36.21125	37.0	37.0	37.0	37.0	37.0
37	36.1405	37.0	37.0	37.0	37.0	37.0
38	36.1455	37.0	37.0	37.0	37.0	37.0
39	36.1505	37.0	37.0	37.0	37.0	37.0
40	36.24275	37.0	37.0	37.0	37.0	37.0
41	35.90945472736368	37.0	37.0	37.0	37.0	37.0
42	36.09982486865149	37.0	37.0	37.0	37.0	37.0
43	35.8740295517155	37.0	37.0	37.0	37.0	37.0
44	36.07025938050869	37.0	37.0	37.0	37.0	37.0
45	35.8878652276915	37.0	37.0	37.0	37.0	37.0
46	35.88380749800585	37.0	37.0	37.0	37.0	37.0
47	36.04914070891515	37.0	37.0	37.0	37.0	37.0
48	36.10178668110449	37.0	37.0	37.0	37.0	37.0
49	36.065534703604406	37.0	37.0	37.0	37.0	37.0
50	35.830240549828176	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	1.0
17	0.0
18	0.0
19	5.0
20	2.0
21	6.0
22	11.0
23	3.0
24	9.0
25	10.0
26	14.0
27	26.0
28	31.0
29	32.0
30	26.0
31	46.0
32	72.0
33	134.0
34	393.0
35	1863.0
36	1137.0
37	177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.69634817408704	29.41470735367684	27.888944472236116
2	34.10852713178294	36.384096024006	15.053763440860216	14.453613403350838
3	27.675	42.175000000000004	15.65	14.499999999999998
4	84.89999999999999	5.75	3.225	6.125
5	71.375	15.2	8.575000000000001	4.8500000000000005
6	64.1	18.875	6.675000000000001	10.35
7	37.475	45.925	7.35	9.25
8	49.575	19.45	10.975	20.0
9	19.35	45.0	24.4	11.25
10	43.7	22.1	15.475	18.725
11	32.475	38.7	15.049999999999999	13.775
12	24.099999999999998	28.325	23.1	24.474999999999998
13	44.0	11.35	26.125	18.525
14	40.1	13.375	25.3	21.224999999999998
15	27.400000000000002	15.299999999999999	47.325	9.975000000000001
16	47.18679669917479	20.180045011252815	23.055763940985248	9.577394348587147
17	23.025000000000002	36.425000000000004	17.05	23.5
18	14.575	40.150000000000006	29.375	15.9
19	18.6	16.875	34.325	30.2
20	13.200000000000001	21.025	32.0	33.775
21	16.204051012753187	46.48662165541385	12.203050762690673	25.10627656914228
22	39.300000000000004	31.574999999999996	12.75	16.375
23	21.7	27.85	20.3	30.15
24	22.2	22.425	11.225	44.15
25	41.125	15.4	11.55	31.924999999999997
26	30.975	20.5	11.55	36.975
27	39.8849712428107	26.6816704176044	19.179794948737182	14.253563390847713
28	39.259814953738434	21.80545136284071	26.38159539884971	12.55313828457114
29	25.75	12.85	46.400000000000006	15.0
30	20.280070017504375	12.253063265816454	54.53863465866466	12.928232058014505
31	15.25	21.975	43.2	19.575
32	32.05801450362591	11.527881970492624	20.880220055013755	35.533883470867714
33	20.349999999999998	11.125	12.9	55.625
34	22.366775081310983	16.937703277458095	23.14235676757568	37.55316487365524
35	11.325000000000001	31.15	22.525000000000002	35.0
36	12.028007001750437	37.65941485371343	32.23305826456614	18.079519879969993
37	15.024999999999999	48.949999999999996	11.75	24.275
38	21.825	16.55	9.325	52.300000000000004
39	41.8	14.7	11.3	32.2
40	25.131282820705174	19.179794948737182	18.079519879969993	37.609402350587644
41	15.182591295647823	39.09454727363682	31.040520260130066	14.682341170585293
42	19.46459844883663	25.769326995246434	33.700275206404804	21.065799349512133
43	27.723516153268218	26.045579764588027	14.775857751064361	31.45504633107939
44	37.67313019390582	12.792747418786199	19.415764291110552	30.118358096197433
45	16.482359136387572	13.217482885729332	32.78041074249605	37.519747235387044
46	27.944695559691574	18.053709119914917	37.19755384206328	16.80404147833023
47	31.80230996508192	11.925866236905721	29.59978511952726	26.672038678485094
48	30.265295073091497	22.414726583649163	25.040606388738496	22.279371954520844
49	30.512514898688913	9.922526817640048	43.89153754469607	15.673420738974968
50	25.15463917525773	13.608247422680412	32.85223367697594	28.38487972508591
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	0.0
7	1.5
8	3.0
9	1.5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	0.5
22	0.0
23	0.0
24	0.0
25	0.0
26	0.5
27	4.0
28	6.5
29	9.0
30	14.0
31	17.0
32	18.0
33	25.0
34	35.0
35	37.5
36	36.0
37	38.5
38	42.5
39	49.5
40	59.0
41	73.0
42	93.0
43	99.0
44	93.5
45	106.5
46	123.0
47	130.0
48	137.0
49	158.0
50	184.0
51	330.5
52	494.5
53	752.5
54	1011.0
55	997.5
56	902.0
57	673.5
58	473.5
59	321.0
60	187.0
61	122.5
62	62.0
63	48.0
64	42.0
65	34.0
66	21.5
67	15.0
68	10.5
69	6.5
70	4.0
71	3.0
72	1.5
73	0.5
74	0.5
75	1.5
76	2.0
77	2.5
78	3.0
79	2.0
80	1.5
81	1.5
82	0.5
83	0.0
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.025
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.025
17	0.0
18	0.0
19	0.0
20	0.0
21	0.025
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.025
28	0.025
29	0.0
30	0.025
31	0.0
32	0.025
33	0.0
34	0.075
35	0.0
36	0.025
37	0.0
38	0.0
39	0.0
40	0.025
41	0.0
42	0.0
43	0.0
44	0.0
45	0.026322716504343247
46	0.0
47	0.02685284640171858
48	0.0
49	0.02978850163836759
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
40	2.0
41	1.0
42	4.0
43	22.0
44	172.0
45	38.0
46	37.0
47	30.0
48	337.0
49	447.0
50	2910.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.48235294117647	44.35
2	5.5058823529411764	5.8500000000000005
3	2.776470588235294	4.425
4	1.5058823529411764	3.2
5	1.3647058823529412	3.6249999999999996
6	0.6588235294117647	2.1
7	0.8941176470588236	3.325
8	0.5176470588235295	2.1999999999999997
9	0.5647058823529412	2.7
>10	2.541176470588235	20.775
>50	0.1411764705882353	4.925
>100	0.047058823529411764	2.5250000000000004
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	101	2.5250000000000004	No Hit
AAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	76	1.9	No Hit
AAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	66	1.6500000000000001	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	55	1.375	No Hit
AAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	40	1.0	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	32	0.8	No Hit
TGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	32	0.8	No Hit
CAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	28	0.7000000000000001	No Hit
CAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	24	0.6	No Hit
CGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	23	0.575	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	20	0.5	No Hit
AATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	20	0.5	No Hit
AAAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	19	0.475	No Hit
TGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	19	0.475	No Hit
TGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	19	0.475	No Hit
AGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	19	0.475	No Hit
CAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	18	0.44999999999999996	No Hit
CGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	18	0.44999999999999996	No Hit
CAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	18	0.44999999999999996	No Hit
CGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	17	0.42500000000000004	No Hit
ATAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	17	0.42500000000000004	No Hit
TTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	16	0.4	No Hit
AGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	16	0.4	No Hit
TGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	15	0.375	No Hit
AGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	15	0.375	No Hit
TTTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	15	0.375	No Hit
TGTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	14	0.35000000000000003	No Hit
AATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	14	0.35000000000000003	No Hit
AGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
CGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
CGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
AGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	13	0.325	No Hit
TAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
ATAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
TGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
ATAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	12	0.3	No Hit
AATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
AACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	12	0.3	No Hit
TGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
TAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	12	0.3	No Hit
CCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
TATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
CACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
CACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
TACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
ACCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
ACAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
CGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
AAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
AACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
TCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
AACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
ATTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
TAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
AAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
AAAGGGAGAGTCCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCA	9	0.22499999999999998	No Hit
AAAGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	9	0.22499999999999998	No Hit
AGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
CTAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
CGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
CATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
TAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
CCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CGCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
ACAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
TCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
CCCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
TTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
AAAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGT	8	0.2	No Hit
ACCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
CACGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
TGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
AGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
CGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
CGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
ATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TTGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TTTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
ACTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
ATCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CTCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
AGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
CCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
ACAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
AACGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
CTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TTCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTA	6	0.15	No Hit
CGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AAAGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCT	6	0.15	No Hit
TCCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
CCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTA	6	0.15	No Hit
AGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
ATAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TTTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CTTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TCGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
AGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCT	5	0.125	No Hit
CCTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
ATCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CCAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
AAAGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGCC	5	0.125	No Hit
TTGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
ACAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
ACCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TGAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CGAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	5	0.125	No Hit
TCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TTGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.025	0.0	0.0	0.0	0.0
23	0.075	0.0	0.0	0.0	0.0
24	0.1	0.0	0.0	0.0	0.0
25	0.15	0.0	0.0	0.0	0.0
26	0.15	0.0	0.0	0.0	0.0
27	0.15	0.0	0.0	0.0	0.0
28	0.15	0.0	0.0	0.0	0.0
29	0.15	0.0	0.0	0.0	0.0
30	0.25	0.0	0.0	0.0	0.0
31	0.275	0.0	0.0	0.0	0.0
32	0.275	0.0	0.0	0.0	0.0
33	0.275	0.0	0.0	0.0	0.0
34	0.275	0.0	0.0	0.0	0.0
35	0.3	0.0	0.0	0.0	0.0
36	0.325	0.0	0.0	0.0	0.0
37	0.35	0.0	0.0	0.0	0.0
38	0.4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCATG	20	9.257044E-5	65.30189	44
GTAGTGC	20	9.257044E-5	65.30189	44
CCTTGTT	85	0.0	46.095448	44
CGTAGTG	25	6.2293455E-4	44.658062	43
GGTGCAT	25	6.2293455E-4	44.658062	43
ACCGTAG	25	0.0017488135	36.431576	41
AGGTGCA	25	0.0017488135	36.431576	42
CCGTAGT	25	0.0017488135	36.431576	42
AAGGTGC	25	0.0017488135	36.431576	41
CACCGTA	25	0.001868013	35.95844	40
CAAGGTG	25	0.001868013	35.95844	40
GCACCGT	25	0.001868013	35.95844	39
CCAAGGT	25	0.001868013	35.95844	39
GGAGATT	25	0.0022646976	34.609997	5
CTTCAGC	25	0.0022646976	34.609997	34
TGTTGCT	25	0.0022646976	34.609997	29
TTGTTGC	25	0.0022646976	34.609997	28
GGCATTT	25	0.0022646976	34.609997	23
GGGCATT	25	0.0022646976	34.609997	22
CAGCACC	25	0.0022646976	34.609997	37
>>END_MODULE
Read 8459 spots for SRR23332739.sra
Written 8459 spots for SRR23332739.sra
Read 8459 spots for SRR23332739.sra
Written 8459 spots for SRR23332739.sra
Read 8464 spots for SRR23332739.sra
Written 8464 spots for SRR23332739.sra
Read 8459 spots for SRR23332739.sra
Written 8459 spots for SRR23332739.sra
Read 8459 spots for SRR23332739.sra
Written 8459 spots for SRR23332739.sra
Read 8459 spots for SRR23332739.sra
Written 8459 spots for SRR23332739.sra
Read 8459 spots for SRR23332739.sra
Written 8459 spots for SRR23332739.sra
Read 8459 spots for SRR23332739.sra
Written 8459 spots for SRR23332739.sra
SRR ids: ['SRR23332739.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_8nk798al
SRR23332739.sra spots: 67677
blocks: [[1, 8459], [8460, 16918], [16919, 25377], [25378, 33836], [33837, 42295], [42296, 50754], [50755, 59213], [59214, 67677]]
SRR23332739 file size 7976
SRR23332739 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR23332739 SRR23332739_1.fastq SRR23332739_2.fastq
Input file:	SRR23332739_1.fastq
Paired file:	SRR23332739_2.fastq
trimmed:	SRR23332739-trimmed-pair1.fastq, SRR23332739-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 02:53:53 2026 >> started

Wed Mar 25 02:53:53 2026 >> done (0.071s)
67677 read pairs processed; of these:
    8 ( 0.01%) short read pairs filtered out after trimming by size control
    0 ( 0.00%) empty read pairs filtered out after trimming by size control
67669 (99.99%) read pairs available; of these:
  462 ( 0.68%) trimmed read pairs available after processing
67207 (99.32%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    2	  0.00%
 19	    4	  0.01%
 20	    9	  0.01%
 21	    1	  0.00%
 22	    3	  0.00%
 23	    6	  0.01%
 24	    3	  0.00%
 25	   14	  0.02%
 26	    8	  0.01%
 27	    5	  0.01%
 28	    8	  0.01%
 29	   12	  0.02%
 30	    8	  0.01%
 31	   11	  0.02%
 32	   17	  0.03%
 33	   14	  0.02%
 34	   16	  0.02%
 35	   34	  0.05%
 36	   28	  0.04%
 37	   19	  0.03%
 38	   30	  0.04%
 39	   35	  0.05%
 40	   90	  0.13%
 41	   66	  0.10%
 42	 3306	  4.89%
 43	 1257	  1.86%
 44	 6055	  8.95%
 45	56608	 83.65%
67669 reads passed initial QC


criterion=sequence-density
sequence-density=9.34
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=21
prefix-density=0.00
prefix-fanout=1.0
sequence=TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACATCCTCGGTACTACATGCTTAGTCAGTCTTTACATTCGCTTGCCAGCTGCGGACGGACACGCCACTAACAAACTAGCCTGATTAAGTTTTAACGCTTCAACCCCAGGCAGGGCTTCCACGCGATCTCTTTTGGGTTTGACCTCTCTTGATCCCCGTCCTAAGAGCGGAGGCTAGGGAGAGAGGGCTCTAAGCAGGTTATTAAGCTGCTAAAGCGTAGTTTTCGTCGTTTGCGACTATTTTTTGCGGCTTTTTACGAGGCCAACCGCCCCTCGGCAT


criterion=fanout-score
sequence-density=0.20
sequence-density-rank=29
fanout-score=4.88
fanout-score-rank=1
prefix-density=1.00
prefix-fanout=1.0
sequence=GTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGACTCCTTGAGAGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGTCTCC


criterion=sequence-density
sequence-density=19.45
sequence-density-rank=1
fanout-score=17.48
fanout-score-rank=2
prefix-density=13.35
prefix-fanout=17.5
sequence=GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTA


criterion=fanout-score
sequence-density=0.52
sequence-density-rank=12
fanout-score=243.53
fanout-score-rank=1
prefix-density=12.21
prefix-fanout=10.4
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTACTCTAACT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACATCCTCGGTACTACATGCTTAGTCAGTCTTTACATTCGCTTGCCAGCTGCGGACGGACACGCCACTAACAAACTAGCCTGATTAAGTTTTAACGCTTCAACCCCAGGCAGGGCTTCCACGCGATCTCTTTTGGGTTTGACCTCTCTTGATCCCCGTCCTAAGAGCGGAGGCTAGGGAGAGAGGGCTCTAAGCAGGTTATTAAGCTGCTAAAGCGTAGTTTTCGTCGTTTGCGACTATTTTTTGCGGCTTTTTACGAGGCCAACCGCCCCTCGGCAT -y GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTA -o SRR23332739 SRR23332739_1.fastq SRR23332739_2.fastq
Input file:	SRR23332739_1.fastq
Paired file:	SRR23332739_2.fastq
trimmed:	SRR23332739-trimmed-pair1.fastq, SRR23332739-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACATCCTCGGTACTACATGCTTA
-- paired 3' end adapter sequence (-y):	GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 02:53:54 2026 >> started

Wed Mar 25 02:53:54 2026 >> done (0.062s)
58647 read pairs processed; of these:
  650 ( 1.11%) short read pairs filtered out after trimming by size control
 6279 (10.71%) empty read pairs filtered out after trimming by size control
51718 (88.19%) read pairs available; of these:
   36 ( 0.07%) trimmed read pairs available after processing
51682 (99.93%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    2	  0.00%
 19	    3	  0.01%
 20	    9	  0.02%
 21	    1	  0.00%
 22	    2	  0.00%
 23	    6	  0.01%
 24	    3	  0.01%
 25	   14	  0.03%
 26	    6	  0.01%
 27	    2	  0.00%
 28	    8	  0.02%
 29	   12	  0.02%
 30	    8	  0.02%
 31	   10	  0.02%
 32	   16	  0.03%
 33	   12	  0.02%
 34	   14	  0.03%
 35	   30	  0.06%
 36	   25	  0.05%
 37	   11	  0.02%
 38	   26	  0.05%
 39	   31	  0.06%
 40	   89	  0.17%
 41	   53	  0.10%
 42	 2780	  5.38%
 43	 1056	  2.04%
 44	 4633	  8.96%
 45	42856	 82.86%


criterion=sequence-density
sequence-density=2.28
sequence-density-rank=1
fanout-score=1.98
fanout-score-rank=9
prefix-density=2.38
prefix-fanout=1.9
sequence=GTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGA


criterion=fanout-score
sequence-density=0.23
sequence-density-rank=29
fanout-score=4.88
fanout-score-rank=1
prefix-density=1.11
prefix-fanout=1.0
sequence=GTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGACTCCTTGAGAGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGTCTCC


criterion=sequence-density
sequence-density=20.78
sequence-density-rank=1
fanout-score=18.20
fanout-score-rank=2
prefix-density=14.58
prefix-fanout=18.2
sequence=GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTA


criterion=fanout-score
sequence-density=0.58
sequence-density-rank=12
fanout-score=243.53
fanout-score-rank=1
prefix-density=13.60
prefix-fanout=10.4
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTACTCTAACT
SRR23332739 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 25 02:54:07
                             Started mapping on |	Mar 25 02:54:07
                                    Finished on |	Mar 25 02:54:25
       Mapping speed, Million of reads per hour |	12.15

                          Number of input reads |	60740
                      Average input read length |	90
                                    UNIQUE READS:
                   Uniquely mapped reads number |	24612
                        Uniquely mapped reads % |	40.52%
                          Average mapped length |	87.37
                       Number of splices: Total |	2582
            Number of splices: Annotated (sjdb) |	3
                       Number of splices: GT/AG |	2365
                       Number of splices: GC/AG |	34
                       Number of splices: AT/AC |	9
               Number of splices: Non-canonical |	174
                      Mismatch rate per base, % |	0.34%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.41
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	15883
             % of reads mapped to multiple loci |	26.15%
        Number of reads mapped to too many loci |	16290
             % of reads mapped to too many loci |	26.82%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.04%
                     % of reads unmapped: other |	0.47%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	20245	20245	20245
N_multimapping	15883	15883	15883
N_noFeature	908	20872	2389
N_ambiguous	3562	675	812
UnstrandedReadsAssigned:20142 PositiveStrandReadsAssigned:3065 NegativeStrandReadsAssigned:21411
Dataset is classified negative stranded
MeadianReadLen=41 20thPercentileLength=41 echo kmer=37
SRR23332739 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR23332739-trimmed-pair1.fastq
                             SRR23332739-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 60,740 reads, 8,428 reads pseudoaligned
[quant] estimated average fragment length: 196.375
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 52 rounds

  4323 SRR23332739.ke.tsv
  4497 SRR23332739.se.tsv
  8820 total
==> SRR23332739.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	9.89474	0	0
AAC73113	2463	2267.63	6	44.1214
AAC73114	933	737.625	1	22.6065
AAC73115	1287	1091.63	3	45.8265
AAC73116	297	124.742	0	0
AAC73117	777	581.974	0	0
AAC73118	1431	1235.63	0	0
AAC73119	954	758.625	7	153.865
AAC73120	588	393.326	5	211.976

==> SRR23332739.se.tsv <==
b0001	1
b0002	6
b0003	0
b0004	3
b0005	0
b0006	0
b0007	0
b0008	7
b0009	5
b0010	0
SRR23332739 completed mapping pipeline successfully
