Starting /dee2/code/volunteer_pipeline.sh SRR23332584
    current disk space = 1439678492
    free memory = 56899596 
SRR23332584 SRAfilesize
ffa17817a00d9e686ebe58c4b9d0e0b3  SRR23332584.sra
SRR23332584.sra file validated
SRR23332584 is paired end
SRR23332584 is conventional basespace
SRR23332584 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332584_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.5535	37.0	37.0	37.0	37.0	37.0
2	36.384	37.0	37.0	37.0	37.0	37.0
3	36.533	37.0	37.0	37.0	37.0	37.0
4	36.5	37.0	37.0	37.0	37.0	37.0
5	36.4955	37.0	37.0	37.0	37.0	37.0
6	36.2315	37.0	37.0	37.0	37.0	37.0
7	36.3225	37.0	37.0	37.0	37.0	37.0
8	36.458	37.0	37.0	37.0	37.0	37.0
9	36.565	37.0	37.0	37.0	37.0	37.0
10	36.4545	37.0	37.0	37.0	37.0	37.0
11	36.417	37.0	37.0	37.0	37.0	37.0
12	36.48	37.0	37.0	37.0	37.0	37.0
13	36.5445	37.0	37.0	37.0	37.0	37.0
14	36.4015	37.0	37.0	37.0	37.0	37.0
15	36.6155	37.0	37.0	37.0	37.0	37.0
16	36.439	37.0	37.0	37.0	37.0	37.0
17	36.3965	37.0	37.0	37.0	37.0	37.0
18	36.3275	37.0	37.0	37.0	37.0	37.0
19	36.4095	37.0	37.0	37.0	37.0	37.0
20	36.3855	37.0	37.0	37.0	37.0	37.0
21	36.403	37.0	37.0	37.0	37.0	37.0
22	36.3425	37.0	37.0	37.0	37.0	37.0
23	36.2965	37.0	37.0	37.0	37.0	37.0
24	36.3635	37.0	37.0	37.0	37.0	37.0
25	36.4875	37.0	37.0	37.0	37.0	37.0
26	36.323	37.0	37.0	37.0	37.0	37.0
27	36.459	37.0	37.0	37.0	37.0	37.0
28	36.452	37.0	37.0	37.0	37.0	37.0
29	36.3005	37.0	37.0	37.0	37.0	37.0
30	36.401	37.0	37.0	37.0	37.0	37.0
31	36.4725	37.0	37.0	37.0	37.0	37.0
32	36.293	37.0	37.0	37.0	37.0	37.0
33	36.38	37.0	37.0	37.0	37.0	37.0
34	36.329	37.0	37.0	37.0	37.0	37.0
35	36.1735	37.0	37.0	37.0	37.0	37.0
36	36.3235	37.0	37.0	37.0	37.0	37.0
37	36.3415	37.0	37.0	37.0	37.0	37.0
38	36.3025	37.0	37.0	37.0	37.0	37.0
39	36.2645	37.0	37.0	37.0	37.0	37.0
40	36.221	37.0	37.0	37.0	37.0	37.0
41	36.2105	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	0.0
24	0.0
25	1.0
26	4.0
27	6.0
28	13.0
29	20.0
30	30.0
31	33.0
32	49.0
33	75.0
34	96.0
35	215.0
36	1387.0
37	2070.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.675	17.299999999999997	47.175	6.8500000000000005
2	28.925	17.325	32.425	21.325
3	30.45	19.875	29.225	20.45
4	21.224999999999998	23.724999999999998	34.325	20.724999999999998
5	30.925000000000004	25.074999999999996	22.025	21.975
6	32.1	23.075000000000003	22.075	22.75
7	23.125	32.074999999999996	20.325	24.474999999999998
8	33.800000000000004	19.325	20.474999999999998	26.400000000000002
9	27.825	16.0	20.75	35.425000000000004
10	25.6	16.525000000000002	30.599999999999998	27.275
11	35.025	18.65	20.375	25.95
12	36.55	20.8	20.125	22.525000000000002
13	26.825	20.275000000000002	20.150000000000002	32.75
14	36.025	20.474999999999998	22.0	21.5
15	34.475	21.325	22.625	21.575
16	37.075	20.925	23.075000000000003	18.925
17	26.150000000000002	31.525	21.825	20.5
18	35.4	21.475	22.400000000000002	20.724999999999998
19	27.125	19.675	31.85	21.349999999999998
20	25.95	21.375	30.349999999999998	22.325
21	36.75	20.625	20.775	21.85
22	27.35	30.5	19.125	23.025000000000002
23	25.525	32.05	19.525000000000002	22.900000000000002
24	24.05	22.25	20.1	33.6
25	27.625	20.549999999999997	19.6	32.225
26	25.45	19.900000000000002	21.45	33.2
27	35.625	18.95	20.549999999999997	24.875
28	25.0	19.1	23.575	32.324999999999996
29	35.525	18.475	23.025000000000002	22.975
30	23.825	21.675	33.300000000000004	21.2
31	24.375	21.475	23.474999999999998	30.675
32	24.3	23.075000000000003	20.825	31.8
33	33.175	23.5	22.775000000000002	20.549999999999997
34	21.45	32.375	22.05	24.125
35	24.375	32.4	21.975	21.25
36	22.7	32.975	22.2	22.125
37	24.0	22.2	32.2	21.6
38	24.575	22.35	29.5	23.575
39	24.2	22.35	21.95	31.5
40	23.875	23.5	20.575	32.05
41	23.45	21.4	30.375000000000004	24.775
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	1.0
16	0.5
17	0.0
18	0.5
19	1.0
20	1.0
21	1.0
22	1.0
23	2.0
24	3.0
25	3.0
26	3.0
27	3.0
28	5.0
29	7.0
30	9.5
31	12.0
32	12.0
33	16.5
34	21.0
35	30.0
36	39.0
37	39.0
38	67.0
39	95.0
40	117.5
41	140.0
42	140.0
43	187.0
44	234.0
45	289.0
46	344.0
47	344.0
48	402.0
49	460.0
50	481.0
51	502.0
52	505.0
53	508.0
54	508.0
55	430.5
56	353.0
57	355.5
58	358.0
59	358.0
60	541.0
61	724.0
62	419.0
63	114.0
64	114.0
65	74.5
66	35.0
67	35.0
68	35.0
69	35.0
70	20.5
71	6.0
72	4.0
73	2.0
74	1.0
75	0.0
76	0.0
77	0.5
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.45
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.02067824648469	45.35
2	16.418527708850288	19.85
3	4.218362282878412	7.6499999999999995
4	1.5715467328370554	3.8
5	1.1579818031430935	3.5000000000000004
6	0.2894954507857734	1.05
7	0.45492142266335817	1.925
8	0.12406947890818859	0.6
9	0.24813895781637718	1.35
>10	0.45492142266335817	5.1499999999999995
>50	0.0	0.0
>100	0.0413564929693962	9.775
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCT	391	9.775	No Hit
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTAC	42	1.05	No Hit
GGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAAC	30	0.75	No Hit
GGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAACC	22	0.5499999999999999	No Hit
AAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGAG	21	0.525	No Hit
GGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTA	17	0.42500000000000004	No Hit
AGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAA	14	0.35000000000000003	No Hit
GCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACA	14	0.35000000000000003	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCC	13	0.325	No Hit
TTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCAC	13	0.325	No Hit
CTAGCCTGATTAAGTTTTAACGCTTCAACCCCAGGCAGGGC	10	0.25	No Hit
AAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTT	10	0.25	No Hit
AAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTC	9	0.22499999999999998	No Hit
GGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAA	9	0.22499999999999998	No Hit
TTGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCC	9	0.22499999999999998	No Hit
TTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGA	9	0.22499999999999998	No Hit
GACTCCAATCCGGAATCGCTAGTAATCGTGGATCAGAATGC	9	0.22499999999999998	No Hit
TTTGAGTTCCCGGGCCTTGTACACACCGCCCGTCACACCAT	9	0.22499999999999998	No Hit
TTTTATCCGTTGAGCTAACCGGTACTAATGAACCGTGTCTC	8	0.2	No Hit
AAAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGA	8	0.2	No Hit
TTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGTCGT	8	0.2	No Hit
TTCCGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTT	7	0.17500000000000002	No Hit
TTCCGACTTCATGACTGGGGTGAAGTCGTAACAAGGTAACC	7	0.17500000000000002	No Hit
TTTATCACTGGAGGAAGGTGGGGATGACGTCAAGTCATCAT	7	0.17500000000000002	No Hit
TCCGGATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACG	7	0.17500000000000002	No Hit
TTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGTCG	7	0.17500000000000002	No Hit
TCTTGATCCCCGTCCTAAGAGCGGAGGCTAGGGAGAGAGGG	7	0.17500000000000002	No Hit
TTGCAGACTCCAATGAAGTCGGAATCGCTAGTAATCGTGGA	7	0.17500000000000002	No Hit
TTTCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTCC	7	0.17500000000000002	No Hit
TTTATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGA	7	0.17500000000000002	No Hit
GGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACAT	7	0.17500000000000002	No Hit
GTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTC	7	0.17500000000000002	No Hit
TCTTCCGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGG	6	0.15	No Hit
GCAATGGATGCTCATTCTCCCTCGCGTACTTGTTCACCATC	6	0.15	No Hit
GTCCTAAGAGCGGAGGCTAGGGAGAGAGGGCTCTAAGCAGG	6	0.15	No Hit
TTTATCCGTTGAGCTAACCGGTACTAATGAACCGTGTCTCC	6	0.15	No Hit
AGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGT	6	0.15	No Hit
TTTATGAGGTCGTAGTCCGGATTGGAGTCTGCAACTCGACT	6	0.15	No Hit
TCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGACT	6	0.15	No Hit
GCCATACCCTTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC	5	0.125	No Hit
TTCCGACTTCATGGATCAGAATGCCACGGTGAATACGTTCC	5	0.125	No Hit
GGAGTCGAGTTGCTAGTAATCGTGGATCAGAATGCCACGGT	5	0.125	No Hit
GGTTAAGCTACCTACTTTGTGATTCATGACTGGGGTGAAGT	5	0.125	No Hit
TGGAGTCGAGTTGCTAGTAATCGTGGATCAGAATGCCACGG	5	0.125	No Hit
TTTACGGCGTGGATCAGAATGCCACGGTGAATACGTTCCCG	5	0.125	No Hit
GGCAGGGCTTCCACGCGATCTCTTTTGGGTTTGACCTCTCT	5	0.125	No Hit
TTTTGGGTTTGACCTCTCTTGATCCCCGTCCTAAGAGCGGA	5	0.125	No Hit
GTCTTCCGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAG	5	0.125	No Hit
GATCCCCGTCCTAAGAGCGGAGGCTAGGGAGAGAGGGCTCT	5	0.125	No Hit
TTTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAG	5	0.125	No Hit
AAAAAACGCGCCCGAAGGCGCGTTGGCGATACACTCAATAT	5	0.125	No Hit
GTGTGTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCA	5	0.125	No Hit
TGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTC	5	0.125	No Hit
TCAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGT	5	0.125	No Hit
TACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACTGG	5	0.125	No Hit
TCATGGAGTCGAGTTGCTAGTAATCGTGGATCAGAATGCCA	5	0.125	No Hit
TCTCGCGAGGTCGGAATCGCTAGTAATCGTGGATCAGAATG	5	0.125	No Hit
TGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAAC	5	0.125	No Hit
TTTCTTTTCCTCGGGGCAGGGTGAGTCGACCCCTAAGGCGA	5	0.125	No Hit
TTGGGTTTGACCTCTCTTGATCCCCGTCCTAAGAGCGGAGG	5	0.125	No Hit
TTACATTCGCTTGCCAGCTGCGGACGGACACGCCACTAACA	5	0.125	No Hit
TCCCCGTCCTAAGAGCGGAGGCTAGGGAGAGAGGGCTCTAA	5	0.125	No Hit
TTCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAG	5	0.125	No Hit
GCGATTCCGACTTCATGACTGGGGTGAAGTCGTAACAAGGT	5	0.125	No Hit
TGCCAGCTGCGGACGGACACGCCACTAACAAACTAGCCTGA	5	0.125	No Hit
AGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCCA	5	0.125	No Hit
GAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.025	0.0	0.0	0.0	0.0
5	0.025	0.0	0.0	0.0	0.0
6	0.05	0.0	0.0	0.0	0.0
7	0.05	0.0	0.0	0.0	0.0
8	0.05	0.0	0.0	0.0	0.0
9	0.05	0.0	0.0	0.0	0.0
10	0.1	0.0	0.0	0.0	0.0
11	0.1	0.0	0.0	0.0	0.0
12	0.1	0.0	0.0	0.0	0.0
13	0.1	0.0	0.0	0.0	0.0
14	0.15	0.0	0.0	0.0	0.0
15	0.175	0.0	0.0	0.0	0.0
16	0.175	0.0	0.0	0.0	0.0
17	0.175	0.0	0.0	0.0	0.0
18	0.175	0.0	0.0	0.0	0.0
19	0.2	0.0	0.0	0.0	0.0
20	0.2	0.0	0.0	0.0	0.0
21	0.225	0.0	0.0	0.0	0.0
22	0.25	0.0	0.0	0.0	0.0
23	0.275	0.0	0.0	0.0	0.0
24	0.325	0.0	0.0	0.0	0.0
25	0.35	0.0	0.0	0.0	0.0
26	0.35	0.0	0.0	0.0	0.0
27	0.375	0.0	0.0	0.0	0.0
28	0.425	0.0	0.0	0.0	0.0
29	0.45	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTCCT	70	5.456968E-12	30.000002	35
GGTGGAG	70	5.456968E-12	30.000002	2
TGGTGGA	70	5.456968E-12	30.000002	1
GTTGAAC	75	1.0913936E-11	28.0	18
AAATTCC	75	1.0913936E-11	28.0	34
GTCCGAA	75	1.0913936E-11	28.0	29
GCTGGCG	75	1.0913936E-11	28.0	8
ACCCGCG	75	1.0913936E-11	28.0	23
TGGAGCT	75	1.0913936E-11	28.0	4
GGGAGTT	75	1.0913936E-11	28.0	14
GAGTTGA	75	1.0913936E-11	28.0	16
CTGGCGG	75	1.0913936E-11	28.0	9
CCCGCGT	75	1.0913936E-11	28.0	24
GAACCCG	75	1.0913936E-11	28.0	21
CGGGAGT	75	1.0913936E-11	28.0	13
CGAAATT	75	1.0913936E-11	28.0	32
AGTTGAA	75	1.0913936E-11	28.0	17
TTGAACC	75	1.0913936E-11	28.0	19
TGGCGGG	75	1.0913936E-11	28.0	10
TGAACCC	75	1.0913936E-11	28.0	20
>>END_MODULE
SRR23332584 read2 length is 38-50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332584_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	38-50
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.687	11.0	11.0	37.0	11.0	37.0
2	22.288	25.0	11.0	37.0	11.0	37.0
3	25.1105	25.0	11.0	37.0	11.0	37.0
4	35.837	37.0	37.0	37.0	37.0	37.0
5	36.127	37.0	37.0	37.0	37.0	37.0
6	35.884	37.0	37.0	37.0	37.0	37.0
7	35.842	37.0	37.0	37.0	37.0	37.0
8	36.0	37.0	37.0	37.0	37.0	37.0
9	35.668	37.0	37.0	37.0	37.0	37.0
10	36.0305	37.0	37.0	37.0	37.0	37.0
11	35.8525	37.0	37.0	37.0	37.0	37.0
12	35.869	37.0	37.0	37.0	37.0	37.0
13	35.9805	37.0	37.0	37.0	37.0	37.0
14	35.997	37.0	37.0	37.0	37.0	37.0
15	35.801	37.0	37.0	37.0	37.0	37.0
16	36.035	37.0	37.0	37.0	37.0	37.0
17	35.959	37.0	37.0	37.0	37.0	37.0
18	36.0075	37.0	37.0	37.0	37.0	37.0
19	35.937	37.0	37.0	37.0	37.0	37.0
20	36.0405	37.0	37.0	37.0	37.0	37.0
21	35.9465	37.0	37.0	37.0	37.0	37.0
22	36.1745	37.0	37.0	37.0	37.0	37.0
23	36.0395	37.0	37.0	37.0	37.0	37.0
24	36.142	37.0	37.0	37.0	37.0	37.0
25	35.928	37.0	37.0	37.0	37.0	37.0
26	36.0685	37.0	37.0	37.0	37.0	37.0
27	36.046	37.0	37.0	37.0	37.0	37.0
28	36.101	37.0	37.0	37.0	37.0	37.0
29	36.0215	37.0	37.0	37.0	37.0	37.0
30	35.9825	37.0	37.0	37.0	37.0	37.0
31	36.009	37.0	37.0	37.0	37.0	37.0
32	35.8465	37.0	37.0	37.0	37.0	37.0
33	35.90725	37.0	37.0	37.0	37.0	37.0
34	36.086	37.0	37.0	37.0	37.0	37.0
35	36.0095	37.0	37.0	37.0	37.0	37.0
36	35.968	37.0	37.0	37.0	37.0	37.0
37	36.0565	37.0	37.0	37.0	37.0	37.0
38	36.0015	37.0	37.0	37.0	37.0	37.0
39	36.089772443110775	37.0	37.0	37.0	37.0	37.0
40	36.067266816704176	37.0	37.0	37.0	37.0	37.0
41	35.808702175543885	37.0	37.0	37.0	37.0	37.0
42	35.905226306576644	37.0	37.0	37.0	37.0	37.0
43	35.75112669003505	37.0	37.0	37.0	37.0	37.0
44	35.933704453441294	37.0	37.0	37.0	37.0	37.0
45	35.85880179466878	37.0	37.0	37.0	37.0	37.0
46	35.96531483457844	37.0	37.0	37.0	37.0	37.0
47	36.09810298102981	37.0	37.0	37.0	37.0	37.0
48	35.940306681270535	37.0	37.0	37.0	37.0	37.0
49	35.84953634460066	37.0	37.0	37.0	37.0	37.0
50	35.83666787922881	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	4.0
16	1.0
17	3.0
18	3.0
19	3.0
20	2.0
21	0.0
22	6.0
23	9.0
24	9.0
25	9.0
26	7.0
27	16.0
28	25.0
29	41.0
30	34.0
31	56.0
32	99.0
33	174.0
34	455.0
35	1755.0
36	1130.0
37	158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	38.550000000000004	25.15	36.3
2	34.517258629314654	32.84142071035518	13.956978489244623	18.684342171085543
3	31.7	36.125	12.075	20.1
4	80.75	7.725	4.475	7.049999999999999
5	67.025	15.525	10.4	7.049999999999999
6	57.75	20.424999999999997	9.4	12.425
7	38.775	39.65	8.75	12.825000000000001
8	43.225	20.4	13.950000000000001	22.425
9	21.15	39.7	25.85	13.3
10	42.0	22.875	16.075	19.05
11	30.175	40.8	15.5	13.525
12	24.375	27.450000000000003	25.275	22.900000000000002
13	39.95	13.875000000000002	27.025	19.15
14	36.625	15.15	23.925	24.3
15	29.025000000000002	16.575	43.925	10.475
16	46.25	20.175	22.95	10.625
17	25.924999999999997	32.85	18.95	22.275
18	15.85	37.95	29.5	16.7
19	19.45	17.299999999999997	34.75	28.499999999999996
20	15.325	21.775	32.324999999999996	30.575000000000003
21	18.609304652326163	42.996498249124556	13.48174087043522	24.912456228114056
22	38.800000000000004	29.849999999999998	14.2	17.150000000000002
23	20.775	29.225	20.825	29.175
24	23.7	22.375	14.124999999999998	39.800000000000004
25	42.6	15.125	13.075000000000001	29.2
26	32.65	19.8	13.025	34.525
27	39.86993496748374	25.512756378189096	18.48424212106053	16.133066533266632
28	36.09304652326163	23.66183091545773	24.087043521760883	16.15807903951976
29	25.674999999999997	14.224999999999998	44.95	15.15
30	18.584292146073036	16.08304152076038	51.52576288144072	13.806903451725864
31	17.45	22.775000000000002	39.475	20.3
32	32.641320660330166	13.406703351675839	18.98449224612306	34.967483741870936
33	21.580395098774694	12.77819454863716	14.87871967991998	50.762690672668164
34	25.437718859429715	17.68384192096048	22.311155577788895	34.567283641820914
35	12.950000000000001	33.5	20.95	32.6
36	14.082041020510255	39.56978489244622	27.51375687843922	18.8344172086043
37	16.2	48.199999999999996	10.925	24.675
38	22.225	17.224999999999998	11.799999999999999	48.75
39	38.5346336584146	16.15403850962741	11.952988247061766	33.35833958489622
40	24.60615153788447	20.7551887971993	18.35458864716179	36.284071017754435
41	17.779444861215303	39.68492123030758	28.182045511377847	14.353588397099276
42	22.280570142535634	27.53188297074269	31.282820705176295	18.904726181545385
43	30.045067601402103	24.937406109163746	15.648472709063595	29.369053580370558
44	36.91801619433198	15.00506072874494	19.458502024291498	28.618421052631575
45	18.738453417788335	15.492214304565849	31.644233306941143	34.12509897070467
46	28.62860192102455	18.38313767342583	34.09818569903949	18.890074706510138
47	29.40379403794038	16.476964769647697	26.666666666666668	27.452574525745256
48	28.313253012048197	25.739320920043813	25.05476451259584	20.89266155531216
49	29.033223585752772	13.409158934450764	41.7539658784795	15.80365160131697
50	29.574390687522733	14.223353946889777	28.9559839941797	27.246271371407783
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	1.0
12	1.5
13	2.5
14	2.5
15	1.5
16	1.0
17	1.0
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	1.0
27	3.5
28	4.5
29	11.0
30	19.0
31	20.0
32	23.0
33	27.5
34	29.0
35	35.5
36	45.0
37	44.0
38	40.0
39	47.5
40	57.0
41	67.5
42	80.0
43	99.0
44	117.0
45	134.0
46	148.5
47	154.0
48	162.0
49	181.5
50	213.5
51	337.5
52	456.0
53	634.0
54	824.5
55	858.0
56	840.0
57	702.5
58	551.5
59	389.5
60	243.0
61	154.0
62	90.0
63	72.0
64	48.5
65	36.0
66	27.0
67	17.0
68	12.0
69	11.0
70	8.5
71	7.5
72	6.0
73	4.5
74	3.5
75	1.5
76	0.0
77	1.5
78	3.0
79	2.5
80	2.0
81	1.0
82	0.0
83	0.5
84	1.0
85	1.0
86	1.0
87	1.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.05
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.05
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.05
28	0.05
29	0.0
30	0.05
31	0.0
32	0.05
33	0.025
34	0.05
35	0.0
36	0.05
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.05982650314089141
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
38	1.0
39	0.0
40	0.0
41	0.0
42	5.0
43	42.0
44	163.0
45	41.0
46	58.0
47	38.0
48	309.0
49	594.0
50	2749.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.724999999999994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.14483047300125	51.449999999999996
2	4.855588112180829	5.800000000000001
3	2.260359983256593	4.05
4	1.4231896190874842	3.4000000000000004
5	0.7534533277521975	2.25
6	0.9627459187944746	3.45
7	0.5860192549183758	2.45
8	0.7534533277521975	3.5999999999999996
9	0.2092925910422771	1.125
>10	1.9254918375889492	17.325
>50	0.12557555462536626	5.1
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	73	1.825	No Hit
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	68	1.7000000000000002	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	63	1.575	No Hit
AAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	47	1.175	No Hit
CTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	28	0.7000000000000001	No Hit
AAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	27	0.675	No Hit
CAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	26	0.65	No Hit
CGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	23	0.575	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	23	0.575	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	21	0.525	No Hit
CCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	20	0.5	No Hit
CGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	19	0.475	No Hit
AACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	19	0.475	No Hit
CGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	19	0.475	No Hit
CGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	17	0.42500000000000004	No Hit
AACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	17	0.42500000000000004	No Hit
CGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
CACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
TGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	14	0.35000000000000003	No Hit
TACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
CCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	14	0.35000000000000003	No Hit
TGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	14	0.35000000000000003	No Hit
CGCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	14	0.35000000000000003	No Hit
CGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
CCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
CCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	12	0.3	No Hit
CCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
CGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
CAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
CGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
TAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
CTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
CGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
ACCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
CCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
CAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	11	0.27499999999999997	No Hit
CGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CCCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
AGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
ACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
AACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
TGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
CCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
AGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
AGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
TAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
ACAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
TAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
CTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
CCGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
CCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
AATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CCTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
AAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGA	8	0.2	No Hit
TCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
AATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
TGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
CGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
TGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
ACAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
AGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
AATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
TCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CCCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
AAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
AAAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAT	7	0.17500000000000002	No Hit
AAAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	7	0.17500000000000002	No Hit
TGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TCGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CTCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
ACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
ATAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AAAGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	6	0.15	No Hit
AGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TTGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
AAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
ACCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
ACAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AACGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
CAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTCAAC	6	0.15	No Hit
CGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TCCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCT	5	0.125	No Hit
TTCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TGTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
ATCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CGCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
AAAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGT	5	0.125	No Hit
AGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CGAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	5	0.125	No Hit
TATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
ACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.025	0.0	0.0	0.0	0.0
8	0.025	0.0	0.0	0.0	0.0
9	0.025	0.0	0.0	0.0	0.0
10	0.025	0.0	0.0	0.0	0.0
11	0.025	0.0	0.0	0.0	0.0
12	0.05	0.0	0.0	0.0	0.0
13	0.05	0.0	0.0	0.0	0.0
14	0.05	0.0	0.0	0.0	0.0
15	0.05	0.0	0.0	0.0	0.0
16	0.05	0.0	0.0	0.0	0.0
17	0.05	0.0	0.0	0.0	0.0
18	0.075	0.0	0.0	0.0	0.0
19	0.125	0.0	0.0	0.0	0.0
20	0.125	0.0	0.0	0.0	0.0
21	0.125	0.0	0.0	0.0	0.0
22	0.125	0.0	0.0	0.0	0.0
23	0.15	0.0	0.0	0.0	0.0
24	0.2	0.0	0.0	0.0	0.0
25	0.2	0.0	0.0	0.0	0.0
26	0.225	0.0	0.0	0.0	0.0
27	0.225	0.0	0.0	0.0	0.0
28	0.25	0.0	0.0	0.0	0.0
29	0.275	0.0	0.0	0.0	0.0
30	0.325	0.0	0.0	0.0	0.0
31	0.325	0.0	0.0	0.0	0.0
32	0.4	0.0	0.0	0.0	0.0
33	0.4	0.0	0.0	0.0	0.0
34	0.425	0.0	0.0	0.0	0.0
35	0.45	0.0	0.0	0.0	0.0
36	0.45	0.0	0.0	0.0	0.0
37	0.475	0.0	0.0	0.0	0.0
38	0.5	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTGCGC	30	8.470744E-6	55.44872	44
CGCTGCG	30	4.4471897E-5	42.401962	43
TCGCTGC	30	8.0968486E-5	38.444447	42
ATCGCTG	30	8.0968486E-5	38.444447	41
AGGGAGA	35	6.345079E-6	37.07143	3
CATCGCT	35	2.0071436E-4	32.95238	40
GCATCGC	35	2.1754151E-4	32.5188	39
CGCATCG	35	2.751601E-4	31.283905	38
AACGCAT	35	2.969559E-4	30.892859	36
ACGCATC	35	2.969559E-4	30.892859	37
TCAACGC	35	2.969559E-4	30.892859	34
CAACGCA	35	2.969559E-4	30.892859	35
CCTTGTT	55	3.0382432E-4	30.244755	44
GTGCATG	45	0.0045795403	29.57265	44
ACCGGTT	40	6.496662E-4	27.03125	24
GGAGACA	40	6.496662E-4	27.03125	5
ACACGGT	40	6.496662E-4	27.03125	9
AGCTCAA	40	6.496662E-4	27.03125	31
GTACCGG	40	6.496662E-4	27.03125	22
GGTTCAT	40	6.496662E-4	27.03125	13
>>END_MODULE
Read 52113 spots for SRR23332584.sra
Written 52113 spots for SRR23332584.sra
Read 52120 spots for SRR23332584.sra
Written 52120 spots for SRR23332584.sra
Read 52113 spots for SRR23332584.sra
Written 52113 spots for SRR23332584.sra
Read 52113 spots for SRR23332584.sra
Written 52113 spots for SRR23332584.sra
Read 52113 spots for SRR23332584.sra
Written 52113 spots for SRR23332584.sra
Read 52113 spots for SRR23332584.sra
Written 52113 spots for SRR23332584.sra
Read 52113 spots for SRR23332584.sra
Written 52113 spots for SRR23332584.sra
Read 52113 spots for SRR23332584.sra
Written 52113 spots for SRR23332584.sra
SRR ids: ['SRR23332584.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_10_7l4xw
SRR23332584.sra spots: 416911
blocks: [[1, 52113], [52114, 104226], [104227, 156339], [156340, 208452], [208453, 260565], [260566, 312678], [312679, 364791], [364792, 416911]]
SRR23332584 file size 49864
SRR23332584 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR23332584 SRR23332584_1.fastq SRR23332584_2.fastq
Input file:	SRR23332584_1.fastq
Paired file:	SRR23332584_2.fastq
trimmed:	SRR23332584-trimmed-pair1.fastq, SRR23332584-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 06:57:57 2026 >> started

Wed Mar 25 06:57:57 2026 >> done (0.433s)
416911 read pairs processed; of these:
   103 ( 0.02%) short read pairs filtered out after trimming by size control
     0 ( 0.00%) empty read pairs filtered out after trimming by size control
416808 (99.98%) read pairs available; of these:
  2200 ( 0.53%) trimmed read pairs available after processing
414608 (99.47%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    24	  0.01%
 19	    28	  0.01%
 20	    45	  0.01%
 21	    20	  0.00%
 22	    19	  0.00%
 23	    35	  0.01%
 24	    24	  0.01%
 25	    40	  0.01%
 26	    35	  0.01%
 27	    41	  0.01%
 28	    27	  0.01%
 29	    47	  0.01%
 30	    68	  0.02%
 31	    70	  0.02%
 32	    81	  0.02%
 33	    68	  0.02%
 34	   125	  0.03%
 35	   165	  0.04%
 36	   169	  0.04%
 37	   122	  0.03%
 38	   127	  0.03%
 39	   154	  0.04%
 40	   347	  0.08%
 41	   469	  0.11%
 42	 18337	  4.40%
 43	  9525	  2.29%
 44	 41776	 10.02%
 45	344820	 82.73%
416808 reads passed initial QC


criterion=sequence-density
sequence-density=1.73
sequence-density-rank=1
fanout-score=1.98
fanout-score-rank=18
prefix-density=1.57
prefix-fanout=2.0
sequence=GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGT


criterion=fanout-score
sequence-density=0.10
sequence-density-rank=28
fanout-score=7.78
fanout-score-rank=1
prefix-density=0.75
prefix-fanout=1.0
sequence=TAGCGATTCCGACTTCATG


criterion=sequence-density
sequence-density=17.46
sequence-density-rank=1
fanout-score=22.87
fanout-score-rank=1
prefix-density=13.10
prefix-fanout=22.9
sequence=GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGA


criterion=fanout-score
sequence-density=17.46
sequence-density-rank=1
fanout-score=22.87
fanout-score-rank=1
prefix-density=13.10
prefix-fanout=22.9
sequence=GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGT -y GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGA -o SRR23332584 SRR23332584_1.fastq SRR23332584_2.fastq
Input file:	SRR23332584_1.fastq
Paired file:	SRR23332584_2.fastq
trimmed:	SRR23332584-trimmed-pair1.fastq, SRR23332584-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGT
-- paired 3' end adapter sequence (-y):	GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 06:57:59 2026 >> started

Wed Mar 25 06:57:59 2026 >> done (0.372s)
333447 read pairs processed; of these:
  3728 ( 1.12%) short read pairs filtered out after trimming by size control
   746 ( 0.22%) empty read pairs filtered out after trimming by size control
328973 (98.66%) read pairs available; of these:
    99 ( 0.03%) trimmed read pairs available after processing
328874 (99.97%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    16	  0.00%
 19	    23	  0.01%
 20	    36	  0.01%
 21	    15	  0.00%
 22	    16	  0.00%
 23	    29	  0.01%
 24	    17	  0.01%
 25	    34	  0.01%
 26	    25	  0.01%
 27	    35	  0.01%
 28	    19	  0.01%
 29	    41	  0.01%
 30	    51	  0.02%
 31	    54	  0.02%
 32	    67	  0.02%
 33	    49	  0.01%
 34	   102	  0.03%
 35	   136	  0.04%
 36	   127	  0.04%
 37	    98	  0.03%
 38	   103	  0.03%
 39	   120	  0.04%
 40	   277	  0.08%
 41	   371	  0.11%
 42	 14660	  4.46%
 43	  7622	  2.32%
 44	 31330	  9.52%
 45	273500	 83.14%


criterion=sequence-density
sequence-density=1.57
sequence-density-rank=1
fanout-score=1.98
fanout-score-rank=21
prefix-density=1.51
prefix-fanout=2.0
sequence=GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGT


criterion=fanout-score
sequence-density=0.10
sequence-density-rank=27
fanout-score=7.64
fanout-score-rank=1
prefix-density=0.73
prefix-fanout=1.0
sequence=TAGCGATTCCGACTTCATG


criterion=sequence-density
sequence-density=16.77
sequence-density-rank=1
fanout-score=23.79
fanout-score-rank=1
prefix-density=12.94
prefix-fanout=23.8
sequence=GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGA


criterion=fanout-score
sequence-density=16.77
sequence-density-rank=1
fanout-score=23.79
fanout-score-rank=1
prefix-density=12.94
prefix-fanout=23.8
sequence=GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGA
SRR23332584 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 25 06:58:13
                             Started mapping on |	Mar 25 06:58:13
                                    Finished on |	Mar 25 06:59:03
       Mapping speed, Million of reads per hour |	29.69

                          Number of input reads |	412334
                      Average input read length |	90
                                    UNIQUE READS:
                   Uniquely mapped reads number |	229477
                        Uniquely mapped reads % |	55.65%
                          Average mapped length |	87.74
                       Number of splices: Total |	14382
            Number of splices: Annotated (sjdb) |	98
                       Number of splices: GT/AG |	13308
                       Number of splices: GC/AG |	266
                       Number of splices: AT/AC |	45
               Number of splices: Non-canonical |	763
                      Mismatch rate per base, % |	0.29%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.28
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	86663
             % of reads mapped to multiple loci |	21.02%
        Number of reads mapped to too many loci |	72970
             % of reads mapped to too many loci |	17.70%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.30%
                     % of reads unmapped: other |	0.33%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	96194	96194	96194
N_multimapping	86663	86663	86663
N_noFeature	6067	205159	15115
N_ambiguous	27893	3988	9522
UnstrandedReadsAssigned:195517 PositiveStrandReadsAssigned:20330 NegativeStrandReadsAssigned:204840
Dataset is classified negative stranded
MeadianReadLen=41 20thPercentileLength=41 echo kmer=37
SRR23332584 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR23332584-trimmed-pair1.fastq
                             SRR23332584-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 412,334 reads, 85,602 reads pseudoaligned
[quant] estimated average fragment length: 222.449
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 74 rounds

  4323 SRR23332584.ke.tsv
  4497 SRR23332584.se.tsv
  8820 total
==> SRR23332584.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	12.7586	22	3875.74
AAC73113	2463	2241.55	16	16.0438
AAC73114	933	711.568	20	63.1755
AAC73115	1287	1065.55	19	40.0788
AAC73116	297	112.161	0	0
AAC73117	777	555.909	0	0
AAC73118	1431	1209.55	0	0
AAC73119	954	732.551	116	355.922
AAC73120	588	369.177	7	42.6186

==> SRR23332584.se.tsv <==
b0001	50
b0002	16
b0003	11
b0004	17
b0005	0
b0006	0
b0007	0
b0008	114
b0009	9
b0010	3
SRR23332584 completed mapping pipeline successfully
