Starting /dee2/code/volunteer_pipeline.sh SRR23332588
    current disk space = 1437065564
    free memory = 57285100 
SRR23332588 SRAfilesize
245ad22185d2df6c39326c1950d8977f  SRR23332588.sra
SRR23332588.sra file validated
SRR23332588 is paired end
SRR23332588 is conventional basespace
SRR23332588 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332588_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.337	37.0	37.0	37.0	37.0	37.0
2	36.405	37.0	37.0	37.0	37.0	37.0
3	36.338	37.0	37.0	37.0	37.0	37.0
4	36.2915	37.0	37.0	37.0	37.0	37.0
5	36.456	37.0	37.0	37.0	37.0	37.0
6	36.317	37.0	37.0	37.0	37.0	37.0
7	36.285	37.0	37.0	37.0	37.0	37.0
8	36.3465	37.0	37.0	37.0	37.0	37.0
9	36.461	37.0	37.0	37.0	37.0	37.0
10	36.357	37.0	37.0	37.0	37.0	37.0
11	36.427	37.0	37.0	37.0	37.0	37.0
12	36.417	37.0	37.0	37.0	37.0	37.0
13	36.2785	37.0	37.0	37.0	37.0	37.0
14	36.3375	37.0	37.0	37.0	37.0	37.0
15	36.418	37.0	37.0	37.0	37.0	37.0
16	36.4575	37.0	37.0	37.0	37.0	37.0
17	36.1425	37.0	37.0	37.0	37.0	37.0
18	36.27	37.0	37.0	37.0	37.0	37.0
19	36.1585	37.0	37.0	37.0	37.0	37.0
20	36.2485	37.0	37.0	37.0	37.0	37.0
21	36.3645	37.0	37.0	37.0	37.0	37.0
22	36.341	37.0	37.0	37.0	37.0	37.0
23	36.239	37.0	37.0	37.0	37.0	37.0
24	36.2385	37.0	37.0	37.0	37.0	37.0
25	36.3335	37.0	37.0	37.0	37.0	37.0
26	36.1905	37.0	37.0	37.0	37.0	37.0
27	36.229	37.0	37.0	37.0	37.0	37.0
28	36.3205	37.0	37.0	37.0	37.0	37.0
29	36.198	37.0	37.0	37.0	37.0	37.0
30	36.256	37.0	37.0	37.0	37.0	37.0
31	36.3005	37.0	37.0	37.0	37.0	37.0
32	36.229	37.0	37.0	37.0	37.0	37.0
33	36.324	37.0	37.0	37.0	37.0	37.0
34	36.3595	37.0	37.0	37.0	37.0	37.0
35	36.217	37.0	37.0	37.0	37.0	37.0
36	35.967	37.0	37.0	37.0	37.0	37.0
37	36.1885	37.0	37.0	37.0	37.0	37.0
38	36.25	37.0	37.0	37.0	37.0	37.0
39	36.114	37.0	37.0	37.0	37.0	37.0
40	36.1945	37.0	37.0	37.0	37.0	37.0
41	36.0995	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	1.0
25	2.0
26	3.0
27	9.0
28	12.0
29	26.0
30	29.0
31	52.0
32	67.0
33	97.0
34	126.0
35	277.0
36	1234.0
37	2064.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.05	23.474999999999998	44.525	8.95
2	32.15	21.85	26.375	19.625
3	35.05	21.224999999999998	25.074999999999996	18.65
4	20.95	26.075	34.575	18.4
5	34.8	25.4	20.375	19.425
6	35.85	23.925	20.65	19.575
7	21.825	37.574999999999996	17.925	22.675
8	37.375	19.400000000000002	18.375	24.85
9	24.2	15.125	19.400000000000002	41.275
10	25.55	14.924999999999999	33.425	26.1
11	38.7	14.75	18.65	27.900000000000002
12	36.449999999999996	18.575	19.8	25.174999999999997
13	25.55	17.075000000000003	18.925	38.45
14	39.15	18.95	20.375	21.525
15	39.475	19.625	20.45	20.45
16	39.95	21.075	20.7	18.275
17	24.125	35.575	21.125	19.175
18	35.9	20.75	25.025	18.325
19	26.174999999999997	19.525000000000002	35.05	19.25
20	25.25	20.200000000000003	33.4	21.15
21	40.975	21.45	17.299999999999997	20.275000000000002
22	23.175	36.8	18.7	21.325
23	22.875	36.075	19.2	21.85
24	24.2	20.5	17.974999999999998	37.325
25	24.95	18.05	20.724999999999998	36.275
26	24.15	17.599999999999998	22.475	35.775
27	37.275000000000006	20.599999999999998	19.425	22.7
28	23.025000000000002	17.1	21.95	37.925
29	35.875	18.125	22.375	23.625
30	20.724999999999998	18.85	38.3	22.125
31	21.25	19.900000000000002	22.675	36.175000000000004
32	23.25	21.175	20.525	35.05
33	37.75	21.75	21.925	18.575
34	21.9	37.225	20.45	20.424999999999997
35	20.05	37.075	23.0	19.875
36	19.725	37.8	22.025	20.45
37	20.724999999999998	22.675	34.65	21.95
38	23.974999999999998	19.55	33.775	22.7
39	23.1	20.525	20.05	36.325
40	21.85	22.825	19.275000000000002	36.05
41	22.1	22.325	33.475	22.1
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	2.0
18	1.0
19	0.0
20	0.0
21	0.0
22	0.0
23	2.0
24	4.0
25	4.0
26	6.5
27	9.0
28	10.5
29	12.0
30	18.0
31	24.0
32	24.0
33	27.5
34	31.0
35	35.5
36	40.0
37	40.0
38	61.0
39	82.0
40	135.5
41	189.0
42	189.0
43	225.5
44	262.0
45	290.0
46	318.0
47	318.0
48	372.0
49	426.0
50	414.0
51	402.0
52	431.0
53	460.0
54	460.0
55	391.5
56	323.0
57	311.0
58	299.0
59	299.0
60	599.0
61	899.0
62	514.5
63	130.0
64	130.0
65	92.5
66	55.0
67	36.0
68	17.0
69	17.0
70	13.5
71	10.0
72	7.5
73	5.0
74	3.0
75	1.0
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.45
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.24474348191758	48.3
2	11.942809083263246	14.2
3	3.406223717409588	6.075
4	1.4297729184188395	3.4000000000000004
5	0.7569386038687973	2.25
6	0.21026072329688814	0.75
7	0.1261564339781329	0.525
8	0.2943650126156434	1.4000000000000001
9	0.0	0.0
>10	0.4625735912531539	5.375
>50	0.08410428931875526	3.15
>100	0.0	0.0
>500	0.04205214465937763	14.575
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCT	583	14.575	No Hit
GGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTA	63	1.575	No Hit
AAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGAG	63	1.575	No Hit
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTAC	38	0.95	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCC	30	0.75	No Hit
AAAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGA	28	0.7000000000000001	No Hit
TGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	22	0.5499999999999999	No Hit
GGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAA	17	0.42500000000000004	No Hit
AAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTT	17	0.42500000000000004	No Hit
AAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTC	16	0.4	No Hit
GCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACA	14	0.35000000000000003	No Hit
AAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGAGG	12	0.3	No Hit
AGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGT	11	0.27499999999999997	No Hit
GGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAAC	10	0.25	No Hit
GGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAACC	8	0.2	No Hit
AAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTT	8	0.2	No Hit
AGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAA	8	0.2	No Hit
AAAAAACGCGCCCGAAGGCGCGTTGGCGATACACTCAATAT	8	0.2	No Hit
AAGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGA	8	0.2	No Hit
TGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACA	8	0.2	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCC	8	0.2	No Hit
GGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	7	0.17500000000000002	No Hit
AAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCG	7	0.17500000000000002	No Hit
TTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCAC	7	0.17500000000000002	No Hit
GCTACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACT	6	0.15	No Hit
TTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGA	6	0.15	No Hit
TTTATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGA	6	0.15	No Hit
ATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCA	6	0.15	No Hit
TTTAGCTACCGAGATGAGTTCTCCCTGACCCTTTAAGGGTC	6	0.15	No Hit
TTCCACGCGATCTCTTTTGGGTTTGACCTCTCTTGATCCCC	5	0.125	No Hit
TAAAAAATGGCGCACAATGTGCGCCATTTTTCACTTCACAG	5	0.125	No Hit
ACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACTGGG	5	0.125	No Hit
TATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAG	5	0.125	No Hit
CTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGTC	5	0.125	No Hit
TTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACT	5	0.125	No Hit
TTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGTCG	5	0.125	No Hit
GGCAGGGCTTCCACGCGATCTCTTTTGGGTTTGACCTCTCT	5	0.125	No Hit
TTGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCC	5	0.125	No Hit
TGTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCT	5	0.125	No Hit
ACAGTGATCCCTGTGAATATTACATCTGCTATACCGATTAA	5	0.125	No Hit
ATTCCTACATCCTCGGTACTACATGCTTAGTCAGTCTTTAC	5	0.125	No Hit
GGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACAT	5	0.125	No Hit
TAAAAAACGCGCCCGAAGGCGCGTTGGCGATACACTCAATA	5	0.125	No Hit
GCCTCGCCTTAGGCGTAGTCGATGGGAAACAGGTTAATATT	5	0.125	No Hit
GAGAGATGACCTTTGCAAAAGGCAAAATTAAGATATAGCAA	5	0.125	No Hit
TTCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTCCT	5	0.125	No Hit
AGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.1	0.0	0.0	0.0	0.0
5	0.125	0.0	0.0	0.0	0.0
6	0.125	0.0	0.0	0.0	0.0
7	0.125	0.0	0.0	0.0	0.0
8	0.125	0.0	0.0	0.0	0.0
9	0.125	0.0	0.0	0.0	0.0
10	0.175	0.0	0.0	0.0	0.0
11	0.225	0.0	0.0	0.0	0.0
12	0.275	0.0	0.0	0.0	0.0
13	0.375	0.0	0.0	0.0	0.0
14	0.425	0.0	0.0	0.0	0.0
15	0.575	0.0	0.0	0.0	0.0
16	0.625	0.0	0.0	0.0	0.0
17	0.9	0.0	0.0	0.0	0.0
18	0.95	0.0	0.0	0.0	0.0
19	0.975	0.0	0.0	0.0	0.0
20	1.1	0.0	0.0	0.0	0.0
21	1.25	0.0	0.0	0.0	0.0
22	1.375	0.0	0.0	0.0	0.0
23	1.425	0.0	0.0	0.0	0.0
24	1.55	0.0	0.0	0.0	0.0
25	1.65	0.0	0.0	0.0	0.0
26	1.7	0.0	0.0	0.0	0.0
27	1.75	0.0	0.0	0.0	0.0
28	1.775	0.0	0.0	0.0	0.0
29	1.925	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTGGA	40	4.0159648E-8	35.0	1
GCGGGAG	45	1.1330303E-7	31.111113	12
GTTGAAC	45	1.1330303E-7	31.111113	18
AAATTCC	45	1.1330303E-7	31.111113	34
AATTCCT	45	1.1330303E-7	31.111113	35
GTCCGAA	45	1.1330303E-7	31.111113	29
GCTGGCG	45	1.1330303E-7	31.111113	8
AACCCGC	45	1.1330303E-7	31.111113	22
ACCCGCG	45	1.1330303E-7	31.111113	23
TGGAGCT	45	1.1330303E-7	31.111113	4
GGGAGTT	45	1.1330303E-7	31.111113	14
GAGTTGA	45	1.1330303E-7	31.111113	16
CTGGCGG	45	1.1330303E-7	31.111113	9
CCCGCGT	45	1.1330303E-7	31.111113	24
GAACCCG	45	1.1330303E-7	31.111113	21
CGGGAGT	45	1.1330303E-7	31.111113	13
GCGTCCG	45	1.1330303E-7	31.111113	27
AGTTGAA	45	1.1330303E-7	31.111113	17
TTGAACC	45	1.1330303E-7	31.111113	19
TGGCGGG	45	1.1330303E-7	31.111113	10
>>END_MODULE
SRR23332588 read2 length is 41-50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332588_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41-50
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.601	11.0	11.0	37.0	11.0	37.0
2	23.0635	25.0	11.0	37.0	11.0	37.0
3	25.878	37.0	11.0	37.0	11.0	37.0
4	36.221	37.0	37.0	37.0	37.0	37.0
5	36.233	37.0	37.0	37.0	37.0	37.0
6	36.0785	37.0	37.0	37.0	37.0	37.0
7	35.9745	37.0	37.0	37.0	37.0	37.0
8	35.9255	37.0	37.0	37.0	37.0	37.0
9	35.98	37.0	37.0	37.0	37.0	37.0
10	36.045	37.0	37.0	37.0	37.0	37.0
11	36.03	37.0	37.0	37.0	37.0	37.0
12	36.061	37.0	37.0	37.0	37.0	37.0
13	36.1375	37.0	37.0	37.0	37.0	37.0
14	36.158	37.0	37.0	37.0	37.0	37.0
15	36.0245	37.0	37.0	37.0	37.0	37.0
16	36.192	37.0	37.0	37.0	37.0	37.0
17	36.1705	37.0	37.0	37.0	37.0	37.0
18	36.233	37.0	37.0	37.0	37.0	37.0
19	36.1835	37.0	37.0	37.0	37.0	37.0
20	36.19	37.0	37.0	37.0	37.0	37.0
21	36.045	37.0	37.0	37.0	37.0	37.0
22	36.3735	37.0	37.0	37.0	37.0	37.0
23	36.184	37.0	37.0	37.0	37.0	37.0
24	36.282	37.0	37.0	37.0	37.0	37.0
25	36.2205	37.0	37.0	37.0	37.0	37.0
26	36.182	37.0	37.0	37.0	37.0	37.0
27	36.116	37.0	37.0	37.0	37.0	37.0
28	36.1525	37.0	37.0	37.0	37.0	37.0
29	36.15	37.0	37.0	37.0	37.0	37.0
30	36.1575	37.0	37.0	37.0	37.0	37.0
31	36.1405	37.0	37.0	37.0	37.0	37.0
32	36.1005	37.0	37.0	37.0	37.0	37.0
33	36.1565	37.0	37.0	37.0	37.0	37.0
34	36.3115	37.0	37.0	37.0	37.0	37.0
35	36.2025	37.0	37.0	37.0	37.0	37.0
36	36.253	37.0	37.0	37.0	37.0	37.0
37	36.019	37.0	37.0	37.0	37.0	37.0
38	36.1365	37.0	37.0	37.0	37.0	37.0
39	36.1085	37.0	37.0	37.0	37.0	37.0
40	36.105	37.0	37.0	37.0	37.0	37.0
41	36.038	37.0	37.0	37.0	37.0	37.0
42	36.004502251125565	37.0	37.0	37.0	37.0	37.0
43	35.95316804407714	37.0	37.0	37.0	37.0	37.0
44	36.01138375917025	37.0	37.0	37.0	37.0	37.0
45	36.02324352879028	37.0	37.0	37.0	37.0	37.0
46	36.04301362543414	37.0	37.0	37.0	37.0	37.0
47	36.05004101722724	37.0	37.0	37.0	37.0	37.0
48	36.07224546722455	37.0	37.0	37.0	37.0	37.0
49	35.94910858995138	37.0	37.0	37.0	37.0	37.0
50	35.711943793911004	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	1.0
20	1.0
21	3.0
22	6.0
23	5.0
24	7.0
25	10.0
26	13.0
27	12.0
28	28.0
29	30.0
30	38.0
31	58.0
32	96.0
33	127.0
34	390.0
35	1680.0
36	1245.0
37	248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	38.025	32.225	29.75
2	36.625	35.125	13.475000000000001	14.774999999999999
3	30.599999999999998	39.574999999999996	16.175	13.65
4	77.425	8.3	6.1	8.175
5	61.925	17.65	12.5	7.925
6	54.125	20.200000000000003	11.75	13.925
7	39.425	36.225	12.275	12.075
8	39.074999999999996	21.45	14.399999999999999	25.074999999999996
9	20.0	36.525	28.549999999999997	14.924999999999999
10	40.325	25.874999999999996	17.075000000000003	16.725
11	26.575	40.75	17.1	15.575
12	23.575	26.150000000000002	28.225	22.05
13	33.0	16.325	30.45	20.225
14	31.35	18.4	24.925	25.324999999999996
15	27.900000000000002	19.2	41.099999999999994	11.799999999999999
16	44.15	22.3	21.675	11.875
17	27.925	31.874999999999996	18.099999999999998	22.1
18	15.375	40.400000000000006	26.200000000000003	18.025
19	20.45	20.1	34.525	24.925
20	16.025	24.15	32.1	27.725
21	17.849999999999998	39.15	14.524999999999999	28.475
22	37.824999999999996	29.349999999999998	14.95	17.875
23	22.575	33.95	17.599999999999998	25.874999999999996
24	23.025000000000002	22.2	14.224999999999998	40.550000000000004
25	40.175	18.95	14.299999999999999	26.575
26	35.775	22.675	14.025000000000002	27.525
27	39.375	23.25	21.85	15.525
28	30.625000000000004	27.325	27.35	14.7
29	21.725	15.975	45.800000000000004	16.5
30	19.975	17.849999999999998	47.825	14.35
31	18.725	23.625	37.6	20.05
32	35.199999999999996	14.424999999999999	20.849999999999998	29.525000000000002
33	22.45	15.15	16.275000000000002	46.125
34	27.325	19.650000000000002	23.425	29.599999999999998
35	14.2	35.625	23.724999999999998	26.450000000000003
36	14.274999999999999	39.550000000000004	26.125	20.05
37	18.25	44.275	12.0	25.474999999999998
38	22.400000000000002	20.075000000000003	11.875	45.65
39	31.15	19.375	14.124999999999998	35.35
40	20.925	22.5	20.9	35.675000000000004
41	20.625	40.525	24.375	14.475
42	23.186593296648326	31.065532766383193	25.087543771885944	20.66033016508254
43	32.632106185825194	24.192336589030806	17.430503380916605	25.7450538442274
44	38.22413356944093	16.39261320516064	20.617252719453578	24.766000505944852
45	19.30797675647121	17.010036978341258	34.601162176439516	29.08082408874802
46	32.11327811915576	20.35800160299225	27.54475020037403	19.98397007747796
47	27.344818156959256	18.020235165436148	23.379819524200162	31.255127153404427
48	25.020920502092046	29.372384937238493	24.211994421199442	21.394700139470014
49	27.423014586709886	13.614262560777956	41.296596434359806	17.666126418152352
50	32.123341139734585	15.26151444184231	27.5175644028103	25.097580015612802
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.5
23	2.0
24	2.5
25	3.0
26	3.0
27	5.0
28	6.5
29	10.5
30	16.5
31	27.5
32	43.5
33	55.0
34	62.5
35	66.5
36	65.5
37	60.5
38	61.0
39	77.5
40	102.0
41	124.0
42	141.5
43	166.5
44	179.5
45	174.5
46	175.5
47	197.5
48	227.0
49	223.5
50	215.5
51	360.0
52	518.5
53	672.5
54	826.0
55	828.0
56	770.5
57	570.0
58	373.5
59	246.5
60	138.0
61	90.0
62	59.5
63	48.5
64	39.0
65	30.5
66	22.0
67	14.5
68	5.5
69	4.0
70	4.0
71	4.0
72	5.0
73	3.0
74	1.0
75	0.5
76	0.0
77	0.5
78	1.0
79	1.0
80	1.0
81	0.5
82	0.0
83	0.5
84	1.0
85	1.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
41	2.0
42	5.0
43	40.0
44	167.0
45	43.0
46	86.0
47	72.0
48	500.0
49	523.0
50	2562.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.050000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.2610797743755	53.525
2	5.439161966156326	6.75
3	2.4174053182917	4.5
4	1.20870265914585	3.0
5	0.7252215954875101	2.25
6	0.7252215954875101	2.7
7	0.4834810636583401	2.1
8	0.684931506849315	3.4000000000000004
9	0.32232070910556004	1.7999999999999998
>10	1.651893634165995	16.475
>50	0.08058017727639001	3.5000000000000004
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	88	2.1999999999999997	No Hit
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	52	1.3	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	47	1.175	No Hit
CAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	35	0.8750000000000001	No Hit
AAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	35	0.8750000000000001	No Hit
AAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	29	0.7250000000000001	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	26	0.65	No Hit
TGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	25	0.625	No Hit
TAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	24	0.6	No Hit
CCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	20	0.5	No Hit
AGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	19	0.475	No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	19	0.475	No Hit
CTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	17	0.42500000000000004	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	16	0.4	No Hit
CGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
CTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCT	16	0.4	No Hit
TTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
CAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	15	0.375	No Hit
ATAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
ACAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
CAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	14	0.35000000000000003	No Hit
TGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	13	0.325	No Hit
AATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
CACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
TGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
AGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	12	0.3	No Hit
AGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGC	12	0.3	No Hit
CGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
TGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
ACCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
TGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
AACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
TGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
CGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	11	0.27499999999999997	No Hit
AGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
TCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
AAAGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCT	10	0.25	No Hit
TATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
CCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
TAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
CGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
TGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
AGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
TCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCT	8	0.2	No Hit
TGTGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
CCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
CGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
CAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
AGACACGGTACTAATGAACCGTGAGGCTTAACCTTAATATGGAC	8	0.2	No Hit
CATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
TCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
TGGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
CCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
AATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
AGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
AAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
AATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
ACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
CGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
ATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CACGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
TCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	7	0.17500000000000002	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTA	6	0.15	No Hit
AGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
AATGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TTCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
AGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TGCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TAGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
CTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AAAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	5	0.125	No Hit
AAAGGGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTAATATGGAC	5	0.125	No Hit
AGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCT	5	0.125	No Hit
AAAGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCT	5	0.125	No Hit
ACGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AAAGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	5	0.125	No Hit
TCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CTCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
ACAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TATGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	5	0.125	No Hit
AGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
ACAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	5	0.125	No Hit
AGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TTTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TAAGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.025	0.0	0.0	0.0	0.0
11	0.1	0.0	0.0	0.0	0.0
12	0.1	0.0	0.0	0.0	0.0
13	0.15	0.0	0.0	0.0	0.0
14	0.15	0.0	0.0	0.0	0.0
15	0.15	0.0	0.0	0.0	0.0
16	0.175	0.0	0.0	0.0	0.0
17	0.175	0.0	0.0	0.0	0.0
18	0.225	0.0	0.0	0.0	0.0
19	0.325	0.0	0.0	0.0	0.0
20	0.5	0.0	0.0	0.0	0.0
21	0.575	0.0	0.0	0.0	0.0
22	0.7	0.0	0.0	0.0	0.0
23	0.775	0.0	0.0	0.0	0.0
24	0.925	0.0	0.0	0.0	0.0
25	0.95	0.0	0.0	0.0	0.0
26	1.075	0.0	0.0	0.0	0.0
27	1.1	0.0	0.0	0.0	0.0
28	1.175	0.0	0.0	0.0	0.0
29	1.3	0.0	0.0	0.0	0.0
30	1.4	0.0	0.0	0.0	0.0
31	1.575	0.0	0.0	0.0	0.0
32	1.725	0.0	0.0	0.0	0.0
33	1.725	0.0	0.0	0.0	0.0
34	1.725	0.0	0.0	0.0	0.0
35	1.825	0.0	0.0	0.0	0.0
36	1.9	0.0	0.0	0.0	0.0
37	1.9	0.0	0.0	0.0	0.0
38	1.95	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGTGC	25	2.047611E-6	70.20409	44
TGACACT	15	0.0019459071	70.20408	44
ATGACAC	15	0.0036076722	60.35088	43
CGTAGTG	25	5.260923E-6	60.350876	43
CACCGTA	25	2.6294581E-5	46.48649	40
ACCGTAG	25	2.6294581E-5	46.48649	41
CCGTAGT	25	2.6294581E-5	46.48649	42
GCACCGT	25	2.8545523E-5	45.866665	39
AGCACCG	25	3.3527802E-5	44.675323	38
GTGCATG	40	3.326636E-5	43.877552	44
GGAGATT	25	4.233588E-5	43.0	5
CTTCAGC	25	4.233588E-5	43.0	34
TGTTGCT	25	4.233588E-5	43.0	29
TTGTTGC	25	4.233588E-5	43.0	28
GGCATTT	25	4.233588E-5	43.0	23
GGGCATT	25	4.233588E-5	43.0	22
CAGCACC	25	4.233588E-5	43.0	37
GCAGGGC	25	4.233588E-5	43.0	19
GAAGCAG	25	4.233588E-5	43.0	16
AGGGCAT	25	4.233588E-5	43.0	21
>>END_MODULE
Read 630789 spots for SRR23332588.sra
Written 630789 spots for SRR23332588.sra
Read 630789 spots for SRR23332588.sra
Written 630789 spots for SRR23332588.sra
Read 630789 spots for SRR23332588.sra
Written 630789 spots for SRR23332588.sra
Read 630794 spots for SRR23332588.sra
Written 630794 spots for SRR23332588.sra
Read 630789 spots for SRR23332588.sra
Written 630789 spots for SRR23332588.sra
Read 630789 spots for SRR23332588.sra
Written 630789 spots for SRR23332588.sra
Read 630789 spots for SRR23332588.sra
Written 630789 spots for SRR23332588.sra
Read 630789 spots for SRR23332588.sra
Written 630789 spots for SRR23332588.sra
SRR ids: ['SRR23332588.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_12rknfbb
SRR23332588.sra spots: 5046317
blocks: [[1, 630789], [630790, 1261578], [1261579, 1892367], [1892368, 2523156], [2523157, 3153945], [3153946, 3784734], [3784735, 4415523], [4415524, 5046317]]
SRR23332588 file size 613836
SRR23332588 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR23332588 SRR23332588_1.fastq SRR23332588_2.fastq
Input file:	SRR23332588_1.fastq
Paired file:	SRR23332588_2.fastq
trimmed:	SRR23332588-trimmed-pair1.fastq, SRR23332588-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 06:43:18 2026 >> started

Wed Mar 25 06:43:21 2026 >> done (3.725s)
5046317 read pairs processed; of these:
   4907 ( 0.10%) short read pairs filtered out after trimming by size control
     11 ( 0.00%) empty read pairs filtered out after trimming by size control
5041399 (99.90%) read pairs available; of these:
  52576 ( 1.04%) trimmed read pairs available after processing
4988823 (98.96%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   1034	  0.02%
 19	   1515	  0.03%
 20	   2035	  0.04%
 21	   1372	  0.03%
 22	   1412	  0.03%
 23	   1501	  0.03%
 24	   1592	  0.03%
 25	   2096	  0.04%
 26	   1832	  0.04%
 27	   1704	  0.03%
 28	   1593	  0.03%
 29	   1974	  0.04%
 30	   1586	  0.03%
 31	   1638	  0.03%
 32	   1659	  0.03%
 33	   1781	  0.04%
 34	   2216	  0.04%
 35	   2347	  0.05%
 36	   2389	  0.05%
 37	   2347	  0.05%
 38	   3331	  0.07%
 39	   3724	  0.07%
 40	   7498	  0.15%
 41	   8491	  0.17%
 42	 238649	  4.73%
 43	 167622	  3.32%
 44	 532292	 10.56%
 45	4044169	 80.22%
5041399 reads passed initial QC


criterion=sequence-density
sequence-density=1.23
sequence-density-rank=1
fanout-score=2.47
fanout-score-rank=16
prefix-density=1.16
prefix-fanout=2.5
sequence=ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG


criterion=fanout-score
sequence-density=1.08
sequence-density-rank=2
fanout-score=9.68
fanout-score-rank=1
prefix-density=1.18
prefix-fanout=8.8
sequence=CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAAT


criterion=sequence-density
sequence-density=17.54
sequence-density-rank=1
fanout-score=20.70
fanout-score-rank=9
prefix-density=14.49
prefix-fanout=20.7
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAA


criterion=fanout-score
sequence-density=0.83
sequence-density-rank=11
fanout-score=111.54
fanout-score-rank=1
prefix-density=7.89
prefix-fanout=11.7
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTAATATGGAC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG -y GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAA -o SRR23332588 SRR23332588_1.fastq SRR23332588_2.fastq
Input file:	SRR23332588_1.fastq
Paired file:	SRR23332588_2.fastq
trimmed:	SRR23332588-trimmed-pair1.fastq, SRR23332588-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG
-- paired 3' end adapter sequence (-y):	GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 06:43:31 2026 >> started

Wed Mar 25 06:43:35 2026 >> done (3.162s)
4033119 read pairs processed; of these:
  40229 ( 1.00%) short read pairs filtered out after trimming by size control
   3218 ( 0.08%) empty read pairs filtered out after trimming by size control
3989672 (98.92%) read pairs available; of these:
   1532 ( 0.04%) trimmed read pairs available after processing
3988140 (99.96%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    832	  0.02%
 19	   1204	  0.03%
 20	   1644	  0.04%
 21	   1111	  0.03%
 22	   1131	  0.03%
 23	   1227	  0.03%
 24	   1270	  0.03%
 25	   1651	  0.04%
 26	   1448	  0.04%
 27	   1359	  0.03%
 28	   1275	  0.03%
 29	   1593	  0.04%
 30	   1280	  0.03%
 31	   1299	  0.03%
 32	   1295	  0.03%
 33	   1433	  0.04%
 34	   1766	  0.04%
 35	   1873	  0.05%
 36	   1936	  0.05%
 37	   1888	  0.05%
 38	   2692	  0.07%
 39	   2992	  0.07%
 40	   5957	  0.15%
 41	   6776	  0.17%
 42	 189842	  4.76%
 43	 134162	  3.36%
 44	 412219	 10.33%
 45	3206517	 80.37%


criterion=sequence-density
sequence-density=1.17
sequence-density-rank=1
fanout-score=2.47
fanout-score-rank=17
prefix-density=1.18
prefix-fanout=2.5
sequence=ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG


criterion=fanout-score
sequence-density=1.09
sequence-density-rank=2
fanout-score=9.88
fanout-score-rank=1
prefix-density=1.20
prefix-fanout=9.0
sequence=CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAAT


criterion=sequence-density
sequence-density=16.82
sequence-density-rank=1
fanout-score=20.50
fanout-score-rank=9
prefix-density=14.20
prefix-fanout=20.5
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAA


criterion=fanout-score
sequence-density=0.83
sequence-density-rank=10
fanout-score=112.18
fanout-score-rank=1
prefix-density=7.87
prefix-fanout=11.8
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTAATATGGAC
SRR23332588 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 25 06:43:53
                             Started mapping on |	Mar 25 06:43:53
                                    Finished on |	Mar 25 06:46:52
       Mapping speed, Million of reads per hour |	100.52

                          Number of input reads |	4997952
                      Average input read length |	89
                                    UNIQUE READS:
                   Uniquely mapped reads number |	3126772
                        Uniquely mapped reads % |	62.56%
                          Average mapped length |	87.76
                       Number of splices: Total |	125237
            Number of splices: Annotated (sjdb) |	1397
                       Number of splices: GT/AG |	109573
                       Number of splices: GC/AG |	2549
                       Number of splices: AT/AC |	735
               Number of splices: Non-canonical |	12380
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.43
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.03
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	804114
             % of reads mapped to multiple loci |	16.09%
        Number of reads mapped to too many loci |	685069
             % of reads mapped to too many loci |	13.71%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.27%
                     % of reads unmapped: other |	0.37%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1067069	1067069	1067069
N_multimapping	804114	804114	804114
N_noFeature	97613	2888231	192515
N_ambiguous	281832	30140	119213
UnstrandedReadsAssigned:2747327 PositiveStrandReadsAssigned:208401 NegativeStrandReadsAssigned:2815044
Dataset is classified negative stranded
MeadianReadLen=41 20thPercentileLength=41 echo kmer=37
SRR23332588 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR23332588-trimmed-pair1.fastq
                             SRR23332588-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,997,952 reads, 1,068,454 reads pseudoaligned
[quant] estimated average fragment length: 208.014
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 148 rounds

  4323 SRR23332588.ke.tsv
  4497 SRR23332588.se.tsv
  8820 total
==> SRR23332588.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	13.0116	197	2920.85
AAC73113	2463	2255.99	167	14.2808
AAC73114	933	726.009	74	19.6635
AAC73115	1287	1079.99	144	25.7227
AAC73116	297	117.987	18	29.4313
AAC73117	777	570.254	28	9.47242
AAC73118	1431	1223.99	85	13.3972
AAC73119	954	747.009	1248	322.3
AAC73120	588	382.51	128	64.5564

==> SRR23332588.se.tsv <==
b0001	327
b0002	154
b0003	69
b0004	136
b0005	18
b0006	29
b0007	84
b0008	1241
b0009	171
b0010	30
SRR23332588 completed mapping pipeline successfully
