Starting /dee2/code/volunteer_pipeline.sh SRR23332740
    current disk space = 1460823256
    free memory = 57831764 
SRR23332740 SRAfilesize
88b3411a68289e97da1cc996f5f60ee5  SRR23332740.sra
SRR23332740.sra file validated
SRR23332740 is paired end
SRR23332740 is conventional basespace
SRR23332740 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332740_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.25	37.0	37.0	37.0	37.0	37.0
2	36.4795	37.0	37.0	37.0	37.0	37.0
3	36.341	37.0	37.0	37.0	37.0	37.0
4	36.4145	37.0	37.0	37.0	37.0	37.0
5	36.3935	37.0	37.0	37.0	37.0	37.0
6	36.2685	37.0	37.0	37.0	37.0	37.0
7	36.394	37.0	37.0	37.0	37.0	37.0
8	36.4165	37.0	37.0	37.0	37.0	37.0
9	36.553	37.0	37.0	37.0	37.0	37.0
10	36.367	37.0	37.0	37.0	37.0	37.0
11	36.445	37.0	37.0	37.0	37.0	37.0
12	36.4355	37.0	37.0	37.0	37.0	37.0
13	36.437	37.0	37.0	37.0	37.0	37.0
14	36.377	37.0	37.0	37.0	37.0	37.0
15	36.555	37.0	37.0	37.0	37.0	37.0
16	36.4955	37.0	37.0	37.0	37.0	37.0
17	36.3525	37.0	37.0	37.0	37.0	37.0
18	36.2635	37.0	37.0	37.0	37.0	37.0
19	36.32	37.0	37.0	37.0	37.0	37.0
20	36.378	37.0	37.0	37.0	37.0	37.0
21	36.4105	37.0	37.0	37.0	37.0	37.0
22	36.342	37.0	37.0	37.0	37.0	37.0
23	36.3445	37.0	37.0	37.0	37.0	37.0
24	36.3285	37.0	37.0	37.0	37.0	37.0
25	36.407	37.0	37.0	37.0	37.0	37.0
26	36.3305	37.0	37.0	37.0	37.0	37.0
27	36.3945	37.0	37.0	37.0	37.0	37.0
28	36.359	37.0	37.0	37.0	37.0	37.0
29	36.3715	37.0	37.0	37.0	37.0	37.0
30	36.389	37.0	37.0	37.0	37.0	37.0
31	36.3885	37.0	37.0	37.0	37.0	37.0
32	36.4245	37.0	37.0	37.0	37.0	37.0
33	36.3545	37.0	37.0	37.0	37.0	37.0
34	36.338	37.0	37.0	37.0	37.0	37.0
35	36.4275	37.0	37.0	37.0	37.0	37.0
36	36.157	37.0	37.0	37.0	37.0	37.0
37	36.176	37.0	37.0	37.0	37.0	37.0
38	36.229	37.0	37.0	37.0	37.0	37.0
39	36.1425	37.0	37.0	37.0	37.0	37.0
40	36.249	37.0	37.0	37.0	37.0	37.0
41	36.1175	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	0.0
25	1.0
26	3.0
27	3.0
28	19.0
29	19.0
30	27.0
31	41.0
32	60.0
33	80.0
34	122.0
35	242.0
36	1260.0
37	2122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.025	20.925	47.0	8.05
2	36.175000000000004	20.275000000000002	24.725	18.825
3	37.574999999999996	20.674999999999997	23.05	18.7
4	21.05	21.825	37.974999999999994	19.15
5	37.1	24.525	18.7	19.675
6	37.925	23.875	18.65	19.55
7	19.75	39.6	17.4	23.25
8	39.4	17.775	18.525	24.3
9	24.325	13.825000000000001	19.925	41.925000000000004
10	24.525	14.325	36.425000000000004	24.725
11	39.45	15.375	19.175	26.0
12	41.6	17.325	18.575	22.5
13	24.975	17.424999999999997	17.150000000000002	40.45
14	42.825	18.75	18.275	20.150000000000002
15	41.525	19.775000000000002	18.35	20.349999999999998
16	42.125	20.974999999999998	20.075000000000003	16.825000000000003
17	25.15	35.375	22.25	17.224999999999998
18	37.225	18.5	24.9	19.375
19	23.45	19.175	37.8	19.575
20	25.2	18.8	35.3	20.7
21	41.075	21.6	18.0	19.325
22	22.05	38.175	17.175	22.6
23	22.2	38.3	17.224999999999998	22.275
24	23.325000000000003	18.625	16.3	41.75
25	25.424999999999997	17.625	17.925	39.025
26	23.125	17.349999999999998	21.6	37.925
27	41.275	16.825000000000003	20.05	21.85
28	22.3	16.675	21.075	39.95
29	38.85	17.5	21.475	22.175
30	20.75	17.675	39.074999999999996	22.5
31	20.599999999999998	18.925	21.05	39.425
32	22.2	20.674999999999997	19.45	37.675
33	39.1	23.200000000000003	19.025	18.675
34	19.35	40.625	21.175	18.85
35	21.15	36.95	21.3	20.599999999999998
36	19.275000000000002	38.4	21.325	21.0
37	20.625	20.575	38.85	19.950000000000003
38	21.875	19.2	35.725	23.200000000000003
39	22.15	20.150000000000002	19.275000000000002	38.425
40	20.775	22.725	18.6	37.9
41	19.575	21.349999999999998	36.625	22.45
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	1.0
16	0.5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0
22	2.0
23	1.5
24	1.0
25	1.0
26	0.5
27	0.0
28	3.5
29	7.0
30	7.5
31	8.0
32	8.0
33	12.5
34	17.0
35	23.0
36	29.0
37	29.0
38	58.5
39	88.0
40	127.5
41	167.0
42	167.0
43	214.5
44	262.0
45	271.0
46	280.0
47	280.0
48	330.0
49	380.0
50	398.0
51	416.0
52	429.0
53	442.0
54	442.0
55	391.0
56	340.0
57	341.0
58	342.0
59	342.0
60	685.5
61	1029.0
62	571.0
63	113.0
64	113.0
65	75.0
66	37.0
67	27.0
68	17.0
69	17.0
70	16.5
71	16.0
72	10.0
73	4.0
74	3.0
75	2.0
76	2.0
77	1.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.95
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.50759606791779	43.925
2	14.78999106344951	16.55
3	3.6193029490616624	6.075
4	1.2957998212689903	2.9000000000000004
5	0.40214477211796246	1.125
6	0.22341376228775692	0.75
7	0.17873100983020554	0.7000000000000001
8	0.08936550491510277	0.4
9	0.044682752457551385	0.22499999999999998
>10	0.7149240393208222	6.525
>50	0.08936550491510277	3.225
>100	0.0	0.0
>500	0.044682752457551385	17.599999999999998
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCT	704	17.599999999999998	No Hit
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTAC	65	1.625	No Hit
GGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTA	64	1.6	No Hit
AAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGAG	39	0.975	No Hit
AAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTT	26	0.65	No Hit
AGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAA	25	0.625	No Hit
AAAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGA	20	0.5	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCC	18	0.44999999999999996	No Hit
GCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACA	15	0.375	No Hit
AAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTC	14	0.35000000000000003	No Hit
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTC	14	0.35000000000000003	TruSeq Adapter, Index 9 (97% over 37bp)
TTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGA	14	0.35000000000000003	No Hit
AAAAAACGCGCCCGAAGGCGCGTTGGCGATACACTCAATAT	13	0.325	No Hit
GGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAAC	12	0.3	No Hit
GGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAA	11	0.27499999999999997	No Hit
CACTAACAAACTAGCCTGATTAAGTTTTAACGCTTCAACCC	10	0.25	No Hit
AGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGT	10	0.25	No Hit
TGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	10	0.25	No Hit
TCTCGTACTAGGAGTGGACGCATCACTGGTGTTCGGGTTGT	10	0.25	No Hit
TGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACA	9	0.22499999999999998	No Hit
GGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACAT	8	0.2	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCC	8	0.2	No Hit
GCTACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACT	7	0.17500000000000002	No Hit
ATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCA	7	0.17500000000000002	No Hit
AAAATGCGCCACCGTGTTAGGGTGGCGCGTGCCCTGCTTTT	7	0.17500000000000002	No Hit
TTCAGCCCCAGGGGTATGGCTGTTCGCCATTTAAAGTGGTA	7	0.17500000000000002	No Hit
GTCGCCCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAAT	6	0.15	No Hit
GGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGC	6	0.15	No Hit
TCATGGAGTCGAGTTGCTAGTAATCGTGGATCAGAATGCCA	6	0.15	No Hit
TTTTAACGCTTCAACCCCAGGCAGGGCTTCCACGCGATCTC	6	0.15	No Hit
TTGGGTTTGACCTCTCTTGATCCCCGTCCTAAGAGCGGAGG	6	0.15	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAAC	5	0.125	No Hit
GCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACTAC	5	0.125	No Hit
TTTCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGA	5	0.125	No Hit
AAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGT	5	0.125	No Hit
TTGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCC	5	0.125	No Hit
TTTTTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAG	5	0.125	No Hit
TTCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	5	0.125	No Hit
AAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCG	5	0.125	No Hit
AGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.025	0.0	0.0	0.0	0.0
3	0.025	0.0	0.0	0.0	0.0
4	0.375	0.0	0.0	0.0	0.0
5	0.4	0.0	0.0	0.0	0.0
6	0.525	0.0	0.0	0.0	0.0
7	0.525	0.0	0.0	0.0	0.0
8	0.525	0.0	0.0	0.0	0.0
9	0.525	0.0	0.0	0.0	0.0
10	0.525	0.0	0.0	0.0	0.0
11	0.55	0.0	0.0	0.0	0.0
12	0.55	0.0	0.0	0.0	0.0
13	0.55	0.0	0.0	0.0	0.0
14	0.55	0.0	0.0	0.0	0.0
15	0.55	0.0	0.0	0.0	0.0
16	0.625	0.0	0.0	0.0	0.0
17	0.625	0.0	0.0	0.0	0.0
18	0.675	0.0	0.0	0.0	0.0
19	0.825	0.0	0.0	0.0	0.0
20	0.925	0.0	0.0	0.0	0.0
21	0.975	0.0	0.0	0.0	0.0
22	0.975	0.0	0.0	0.0	0.0
23	1.075	0.0	0.0	0.0	0.0
24	1.125	0.0	0.0	0.0	0.0
25	1.175	0.0	0.0	0.0	0.0
26	1.25	0.0	0.0	0.0	0.0
27	1.325	0.0	0.0	0.0	0.0
28	1.375	0.0	0.0	0.0	0.0
29	1.475	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTGGAG	65	0.0	35.0	2
TGGTGGA	65	0.0	35.0	1
GTTGAAC	80	0.0	28.4375	18
GAAATTC	80	0.0	28.4375	33
AAATTCC	80	0.0	28.4375	34
AATTCCT	80	0.0	28.4375	35
GTCCGAA	80	0.0	28.4375	29
GAGCTGG	80	0.0	28.4375	6
GAGTTGA	80	0.0	28.4375	16
CCCGCGT	80	0.0	28.4375	24
GCGTCCG	80	0.0	28.4375	27
CGAAATT	80	0.0	28.4375	32
AGTTGAA	80	0.0	28.4375	17
TTGAACC	80	0.0	28.4375	19
TGAACCC	80	0.0	28.4375	20
GGAGCTG	80	0.0	28.4375	5
CGCGTCC	80	0.0	28.4375	26
CGTCCGA	80	0.0	28.4375	28
CCGCGTC	80	0.0	28.4375	25
GGGAGTT	75	1.0913936E-11	28.0	14
>>END_MODULE
SRR23332740 read2 length is 23-50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332740_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	23-50
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.509	11.0	11.0	37.0	11.0	37.0
2	22.166	25.0	11.0	37.0	11.0	37.0
3	24.9675	25.0	11.0	37.0	11.0	37.0
4	36.022	37.0	37.0	37.0	37.0	37.0
5	36.1935	37.0	37.0	37.0	37.0	37.0
6	36.0175	37.0	37.0	37.0	37.0	37.0
7	35.851	37.0	37.0	37.0	37.0	37.0
8	35.902	37.0	37.0	37.0	37.0	37.0
9	35.9735	37.0	37.0	37.0	37.0	37.0
10	36.023	37.0	37.0	37.0	37.0	37.0
11	35.976	37.0	37.0	37.0	37.0	37.0
12	36.031	37.0	37.0	37.0	37.0	37.0
13	36.1175	37.0	37.0	37.0	37.0	37.0
14	36.1185	37.0	37.0	37.0	37.0	37.0
15	35.8655	37.0	37.0	37.0	37.0	37.0
16	36.067	37.0	37.0	37.0	37.0	37.0
17	36.169	37.0	37.0	37.0	37.0	37.0
18	36.245	37.0	37.0	37.0	37.0	37.0
19	36.214	37.0	37.0	37.0	37.0	37.0
20	36.154	37.0	37.0	37.0	37.0	37.0
21	36.1155	37.0	37.0	37.0	37.0	37.0
22	36.2625	37.0	37.0	37.0	37.0	37.0
23	36.1895	37.0	37.0	37.0	37.0	37.0
24	36.252313078269566	37.0	37.0	37.0	37.0	37.0
25	36.069267316829205	37.0	37.0	37.0	37.0	37.0
26	36.15803950987747	37.0	37.0	37.0	37.0	37.0
27	36.0942735683921	37.0	37.0	37.0	37.0	37.0
28	36.113528382095524	37.0	37.0	37.0	37.0	37.0
29	36.13828457114278	37.0	37.0	37.0	37.0	37.0
30	36.14803700925231	37.0	37.0	37.0	37.0	37.0
31	36.307326831707925	37.0	37.0	37.0	37.0	37.0
32	36.06901725431358	37.0	37.0	37.0	37.0	37.0
33	36.02375593898475	37.0	37.0	37.0	37.0	37.0
34	36.138534633658416	37.0	37.0	37.0	37.0	37.0
35	36.07926981745436	37.0	37.0	37.0	37.0	37.0
36	36.29732433108277	37.0	37.0	37.0	37.0	37.0
37	36.15128782195549	37.0	37.0	37.0	37.0	37.0
38	36.115278819704926	37.0	37.0	37.0	37.0	37.0
39	36.03575893973493	37.0	37.0	37.0	37.0	37.0
40	36.08027006751688	37.0	37.0	37.0	37.0	37.0
41	35.9437359339835	37.0	37.0	37.0	37.0	37.0
42	36.07003501750876	37.0	37.0	37.0	37.0	37.0
43	35.90708740295517	37.0	37.0	37.0	37.0	37.0
44	35.964195663136664	37.0	37.0	37.0	37.0	37.0
45	36.002082248828735	37.0	37.0	37.0	37.0	37.0
46	35.93700787401575	37.0	37.0	37.0	37.0	37.0
47	36.00026766595289	37.0	37.0	37.0	37.0	37.0
48	36.148288973384034	37.0	37.0	37.0	37.0	37.0
49	36.195795795795796	37.0	37.0	37.0	37.0	37.0
50	35.79079798705967	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	1.0
16	0.0
17	2.0
18	1.0
19	5.0
20	1.0
21	2.0
22	5.0
23	5.0
24	8.0
25	14.0
26	9.0
27	13.0
28	18.0
29	23.0
30	45.0
31	73.0
32	85.0
33	149.0
34	408.0
35	1725.0
36	1229.0
37	178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	40.225	29.525000000000002	30.25
2	30.8	40.849999999999994	13.725000000000001	14.625
3	27.975	41.525	15.425	15.075
4	79.57499999999999	7.425	5.625	7.375
5	64.14999999999999	16.400000000000002	12.049999999999999	7.3999999999999995
6	58.675	19.275000000000002	9.175	12.875
7	41.775	36.3	9.049999999999999	12.875
8	37.075	21.125	13.450000000000001	28.349999999999998
9	21.325	35.35	29.625	13.700000000000001
10	41.825	24.725	15.925	17.525
11	26.3	43.325	16.625	13.750000000000002
12	22.275	25.1	30.425	22.2
13	33.475	14.124999999999998	33.5	18.9
14	31.0	17.025000000000002	23.625	28.349999999999998
15	27.05	16.8	45.65	10.5
16	46.050000000000004	19.6	22.875	11.475
17	30.049999999999997	31.624999999999996	16.85	21.475
18	14.725	43.7	25.85	15.725
19	18.55	18.35	39.825	23.275000000000002
20	14.6	22.125	35.925000000000004	27.35
21	17.849999999999998	37.824999999999996	12.8	31.525
22	40.949999999999996	28.299999999999997	13.350000000000001	17.4
23	21.55	35.05	18.95	24.45
24	22.20555138784696	22.155538884721178	12.928232058014505	42.71067766941735
25	42.23555888972243	16.404101025256317	13.178294573643413	28.182045511377847
26	38.3191595797899	20.66033016508254	13.081540770385192	27.938969484742373
27	42.63565891472868	22.455613903475868	20.7551887971993	14.153538384596148
28	30.940470235117555	30.115057528764382	26.313156578289142	12.631315657828916
29	20.580145036259065	14.528632158039509	50.862715678919734	14.028507126781694
30	20.135067533766886	15.332666333166584	51.92596298149075	12.606303151575787
31	17.60440110027507	22.05551387846962	40.835208802200555	19.504876219054765
32	37.843921960980495	13.731865932966484	19.259629814907452	29.164582291145575
33	21.80545136284071	12.978244561140285	14.803700925231308	50.412603150787696
34	30.665332666333168	19.084542271135568	22.18609304652326	28.064032016008007
35	12.978244561140285	39.15978994748687	20.80520130032508	27.056764191047762
36	14.078519629907477	42.63565891472868	25.206301575393848	18.079519879969993
37	16.579144786196547	47.586896724181045	11.677919479869967	24.15603900975244
38	21.705426356589147	17.404351087771943	11.40285071267817	49.48737184296074
39	32.883220805201304	16.55413853463366	12.328082020505127	38.234558639659916
40	19.679919979995	21.780445111277817	17.22930732683171	41.31032758189547
41	16.829207301825456	44.78619654913729	22.95573893473368	15.428857214303576
42	21.46073036518259	33.16658329164582	25.137568784392194	20.23511755877939
43	35.81267217630854	22.539444027047335	14.825945404457801	26.82193839218633
44	41.57841654059506	15.481593545133638	19.162884518406457	23.77710539586485
45	17.985424258198854	16.29359708485164	36.413326392503905	29.3076522644456
46	33.727034120734906	18.503937007874015	27.821522309711288	19.94750656167979
47	26.532797858099062	17.188755020080322	22.11512717536814	34.163319946452475
48	23.465507876154266	32.40086909288431	22.705051602390007	21.428571428571427
49	28.85885885885886	12.432432432432433	42.82282282282282	15.885885885885887
50	36.77210639827462	13.98274622573688	24.04744787922358	25.19769949676492
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	0.5
10	1.0
11	1.0
12	1.0
13	0.5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	1.5
22	2.0
23	2.0
24	2.0
25	2.5
26	4.0
27	7.5
28	9.5
29	17.0
30	29.0
31	32.5
32	31.5
33	35.0
34	43.5
35	51.5
36	56.0
37	57.5
38	55.5
39	65.0
40	80.0
41	86.5
42	98.0
43	116.5
44	128.5
45	140.5
46	151.5
47	163.5
48	177.0
49	208.5
50	242.0
51	428.5
52	637.5
53	786.5
54	927.0
55	849.5
56	722.5
57	552.0
58	368.0
59	226.0
60	120.0
61	92.0
62	68.0
63	52.5
64	38.5
65	28.0
66	19.5
67	15.0
68	12.0
69	10.5
70	9.0
71	7.0
72	4.0
73	3.0
74	2.5
75	1.0
76	0.0
77	0.5
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.5
84	1.0
85	0.5
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.025006251562890724
27	0.0
28	0.025006251562890724
29	0.0
30	0.025006251562890724
31	0.0
32	0.025006251562890724
33	0.0
34	0.025006251562890724
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.02676659528907923
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
23	1.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	1.0
42	5.0
43	27.0
44	124.0
45	32.0
46	74.0
47	54.0
48	352.0
49	548.0
50	2782.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.20835090679039	51.1
2	4.9768030366933775	5.8999999999999995
3	2.066638549135386	3.675
4	1.7714044706874739	4.2
5	1.2652889076339098	3.75
6	0.7169970476592155	2.55
7	0.5904681568958245	2.45
8	0.42176296921130324	2.0
9	0.25305778152678193	1.35
>10	1.687051876845213	19.825
>50	0.0	0.0
>100	0.04217629692113033	3.2
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	128	3.2	No Hit
CAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	50	1.25	No Hit
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	49	1.225	No Hit
TAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	42	1.05	No Hit
AAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	41	1.0250000000000001	No Hit
AAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	33	0.8250000000000001	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	30	0.75	No Hit
CGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	26	0.65	No Hit
AACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	26	0.65	No Hit
CCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	24	0.6	No Hit
CGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	23	0.575	No Hit
AATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	23	0.575	No Hit
TAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	20	0.5	No Hit
ACAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	20	0.5	No Hit
TACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	18	0.44999999999999996	No Hit
ATAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	18	0.44999999999999996	No Hit
CAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	18	0.44999999999999996	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	17	0.42500000000000004	No Hit
CGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	17	0.42500000000000004	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	17	0.42500000000000004	No Hit
CTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
TATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
AGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
TAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	16	0.4	No Hit
TAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
TGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
AAAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	14	0.35000000000000003	No Hit
CACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
AGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
ACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
TGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	14	0.35000000000000003	No Hit
AATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
TGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	13	0.325	No Hit
CATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
CTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
AAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
CCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
CGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
AGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
CCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
ACCCTACAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCAGA	10	0.25	Illumina Single End PCR Primer 1 (96% over 33bp)
CGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
TGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
CTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
AATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
TCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
AAAGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	8	0.2	No Hit
CAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
CACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
TGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
ACTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
AAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
ATAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCT	7	0.17500000000000002	No Hit
AAAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGT	7	0.17500000000000002	No Hit
TTTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
AGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
AAAGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCT	7	0.17500000000000002	No Hit
TGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
CCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
ACAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
AACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TTGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AAAGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCT	6	0.15	No Hit
CCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
ACCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
CCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CAAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	6	0.15	No Hit
ATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
ACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TGCGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	6	0.15	No Hit
AAAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAT	5	0.125	No Hit
CCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CTTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AGACACGGTACTAATGAACCGTGAGGCTTAACCTTACCCTACAG	5	0.125	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCT	5	0.125	No Hit
TTGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TTCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
ATAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CGTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TTGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TGGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CAAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAT	5	0.125	No Hit
CATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	5	0.125	No Hit
TTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	5	0.125	No Hit
TATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
AAAGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTC	5	0.125	No Hit
CAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.25	0.0	0.0	0.0	0.0
11	0.375	0.0	0.0	0.0	0.0
12	0.4	0.0	0.0	0.0	0.0
13	0.475	0.0	0.0	0.0	0.0
14	0.5	0.0	0.0	0.0	0.0
15	0.6	0.0	0.0	0.0	0.0
16	0.6	0.0	0.0	0.0	0.0
17	0.6	0.0	0.0	0.0	0.0
18	0.6	0.0	0.0	0.0	0.0
19	0.625	0.0	0.0	0.0	0.0
20	0.65	0.0	0.0	0.0	0.0
21	0.7	0.0	0.0	0.0	0.0
22	0.7	0.0	0.0	0.0	0.0
23	0.7	0.0	0.0	0.0	0.0
24	0.725	0.0	0.0	0.0	0.0
25	0.75	0.0	0.0	0.0	0.0
26	0.75	0.0	0.0	0.0	0.0
27	0.825	0.0	0.0	0.0	0.0
28	1.0	0.0	0.0	0.0	0.0
29	1.075	0.0	0.0	0.0	0.0
30	1.125	0.0	0.0	0.0	0.0
31	1.2	0.0	0.0	0.0	0.0
32	1.3	0.0	0.0	0.0	0.0
33	1.45	0.0	0.0	0.0	0.0
34	1.45	0.0	0.0	0.0	0.0
35	1.5	0.0	0.0	0.0	0.0
36	1.55	0.0	0.0	0.0	0.0
37	1.6	0.0	0.0	0.0	0.0
38	1.65	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCACCT	15	0.0030658948	62.818184	44
AACCACC	15	0.0068331063	51.567165	43
GTGCATG	55	4.5474735E-11	51.396694	44
GAACCAC	15	0.009139828	47.98611	42
GGAACCA	15	0.009662969	47.32877	41
AAGGGAG	20	7.6209044E-4	43.187496	2
AAAGGGA	20	7.6209044E-4	43.187496	1
AGCTGAA	20	7.6209044E-4	43.187496	3
TGAAAAA	20	7.6209044E-4	43.187496	6
AGGGAGA	35	6.408438E-6	37.017857	3
CCTTGTT	55	9.644515E-6	34.26446	44
AGGTGCA	65	4.0417945E-9	33.221153	42
AAGGTGC	65	4.667527E-9	32.76607	41
CAAGGTG	65	5.3769327E-9	32.323284	40
CCAAGGT	65	7.0958777E-9	31.472672	39
GGTGCAT	75	7.717972E-9	30.9403	43
TCGACGG	70	6.4210326E-10	30.848213	20
GAAACCC	70	6.4210326E-10	30.848213	34
GACGGGA	70	6.4210326E-10	30.848213	22
TTCGACG	70	6.4210326E-10	30.848213	19
>>END_MODULE
Read 427437 spots for SRR23332740.sra
Written 427437 spots for SRR23332740.sra
Read 427437 spots for SRR23332740.sra
Written 427437 spots for SRR23332740.sra
Read 427437 spots for SRR23332740.sra
Written 427437 spots for SRR23332740.sra
Read 427437 spots for SRR23332740.sra
Written 427437 spots for SRR23332740.sra
Read 427437 spots for SRR23332740.sra
Written 427437 spots for SRR23332740.sra
Read 427437 spots for SRR23332740.sra
Written 427437 spots for SRR23332740.sra
Read 427437 spots for SRR23332740.sra
Written 427437 spots for SRR23332740.sra
Read 427442 spots for SRR23332740.sra
Written 427442 spots for SRR23332740.sra
SRR ids: ['SRR23332740.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_y611i58w
SRR23332740.sra spots: 3419501
blocks: [[1, 427437], [427438, 854874], [854875, 1282311], [1282312, 1709748], [1709749, 2137185], [2137186, 2564622], [2564623, 2992059], [2992060, 3419501]]
SRR23332740 file size 415252
SRR23332740 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR23332740 SRR23332740_1.fastq SRR23332740_2.fastq
Input file:	SRR23332740_1.fastq
Paired file:	SRR23332740_2.fastq
trimmed:	SRR23332740-trimmed-pair1.fastq, SRR23332740-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 02:54:31 2026 >> started

Wed Mar 25 02:54:33 2026 >> done (2.128s)
3419501 read pairs processed; of these:
    792 ( 0.02%) short read pairs filtered out after trimming by size control
      5 ( 0.00%) empty read pairs filtered out after trimming by size control
3418704 (99.98%) read pairs available; of these:
  23099 ( 0.68%) trimmed read pairs available after processing
3395605 (99.32%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    189	  0.01%
 19	    296	  0.01%
 20	    360	  0.01%
 21	    375	  0.01%
 22	    367	  0.01%
 23	    367	  0.01%
 24	    462	  0.01%
 25	    632	  0.02%
 26	    522	  0.02%
 27	    593	  0.02%
 28	    492	  0.01%
 29	   1958	  0.06%
 30	    539	  0.02%
 31	    772	  0.02%
 32	    663	  0.02%
 33	    764	  0.02%
 34	   1053	  0.03%
 35	   1127	  0.03%
 36	    878	  0.03%
 37	    922	  0.03%
 38	   1719	  0.05%
 39	   1867	  0.05%
 40	   4848	  0.14%
 41	   3791	  0.11%
 42	 135060	  3.95%
 43	  79601	  2.33%
 44	 296882	  8.68%
 45	2881605	 84.29%
3418704 reads passed initial QC


criterion=sequence-density
sequence-density=1.08
sequence-density-rank=1
fanout-score=1.96
fanout-score-rank=22
prefix-density=0.90
prefix-fanout=2.0
sequence=GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCG


criterion=fanout-score
sequence-density=0.26
sequence-density-rank=16
fanout-score=7.06
fanout-score-rank=1
prefix-density=1.84
prefix-fanout=1.0
sequence=TTCATTAGTACGAGAGGACCGGGATGGACGCACCGCTGGTG


criterion=sequence-density
sequence-density=20.40
sequence-density-rank=1
fanout-score=17.66
fanout-score-rank=10
prefix-density=17.56
prefix-fanout=17.7
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCA


criterion=fanout-score
sequence-density=0.65
sequence-density-rank=14
fanout-score=159.89
fanout-score-rank=1
prefix-density=9.01
prefix-fanout=11.5
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTACCCTACAG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCG -y GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCA -o SRR23332740 SRR23332740_1.fastq SRR23332740_2.fastq
Input file:	SRR23332740_1.fastq
Paired file:	SRR23332740_2.fastq
trimmed:	SRR23332740-trimmed-pair1.fastq, SRR23332740-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAG
-- paired 3' end adapter sequence (-y):	GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 02:54:40 2026 >> started

Wed Mar 25 02:54:42 2026 >> done (2.027s)
2848920 read pairs processed; of these:
  29240 ( 1.03%) short read pairs filtered out after trimming by size control
   3113 ( 0.11%) empty read pairs filtered out after trimming by size control
2816567 (98.86%) read pairs available; of these:
    989 ( 0.04%) trimmed read pairs available after processing
2815578 (99.96%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    158	  0.01%
 19	    249	  0.01%
 20	    307	  0.01%
 21	    296	  0.01%
 22	    314	  0.01%
 23	    296	  0.01%
 24	    384	  0.01%
 25	    505	  0.02%
 26	    437	  0.02%
 27	    499	  0.02%
 28	    405	  0.01%
 29	   1619	  0.06%
 30	    444	  0.02%
 31	    648	  0.02%
 32	    547	  0.02%
 33	    638	  0.02%
 34	    881	  0.03%
 35	    954	  0.03%
 36	    733	  0.03%
 37	    771	  0.03%
 38	   1439	  0.05%
 39	   1521	  0.05%
 40	   4054	  0.14%
 41	   3174	  0.11%
 42	 112352	  3.99%
 43	  66349	  2.36%
 44	 238459	  8.47%
 45	2378134	 84.43%


criterion=sequence-density
sequence-density=1.01
sequence-density-rank=1
fanout-score=1.97
fanout-score-rank=22
prefix-density=0.91
prefix-fanout=2.0
sequence=GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCG


criterion=fanout-score
sequence-density=0.26
sequence-density-rank=16
fanout-score=7.05
fanout-score-rank=1
prefix-density=1.85
prefix-fanout=1.0
sequence=TTCATTAGTACGAGAGGACCGGGATGGACGCACCGCTGGTGTACCAGTTGTTC


criterion=sequence-density
sequence-density=19.74
sequence-density-rank=1
fanout-score=17.29
fanout-score-rank=10
prefix-density=17.28
prefix-fanout=17.3
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC


criterion=fanout-score
sequence-density=0.66
sequence-density-rank=16
fanout-score=158.76
fanout-score-rank=1
prefix-density=9.06
prefix-fanout=11.6
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTACCCTACAG
SRR23332740 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 25 02:54:58
                             Started mapping on |	Mar 25 02:54:58
                                    Finished on |	Mar 25 02:56:34
       Mapping speed, Million of reads per hour |	126.99

                          Number of input reads |	3386351
                      Average input read length |	90
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2048322
                        Uniquely mapped reads % |	60.49%
                          Average mapped length |	87.81
                       Number of splices: Total |	89677
            Number of splices: Annotated (sjdb) |	695
                       Number of splices: GT/AG |	80431
                       Number of splices: GC/AG |	1757
                       Number of splices: AT/AC |	401
               Number of splices: Non-canonical |	7088
                      Mismatch rate per base, % |	0.24%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.41
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	592402
             % of reads mapped to multiple loci |	17.49%
        Number of reads mapped to too many loci |	551604
             % of reads mapped to too many loci |	16.29%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.40%
                     % of reads unmapped: other |	0.33%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	745627	745627	745627
N_multimapping	592402	592402	592402
N_noFeature	68679	1897987	129929
N_ambiguous	175132	20706	72740
UnstrandedReadsAssigned:1804511 PositiveStrandReadsAssigned:129629 NegativeStrandReadsAssigned:1845653
Dataset is classified negative stranded
MeadianReadLen=41 20thPercentileLength=41 echo kmer=37
SRR23332740 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR23332740-trimmed-pair1.fastq
                             SRR23332740-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,386,351 reads, 616,743 reads pseudoaligned
[quant] estimated average fragment length: 222.955
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 52 rounds

  4323 SRR23332740.ke.tsv
  4497 SRR23332740.se.tsv
  8820 total
==> SRR23332740.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	14.039	142	3011.16
AAC73113	2463	2241.04	121	16.0737
AAC73114	933	711.07	70	29.3067
AAC73115	1287	1065.04	131	36.6171
AAC73116	297	114.776	10	25.9376
AAC73117	777	555.427	24	12.8637
AAC73118	1431	1209.04	19	4.67834
AAC73119	954	732.07	667	271.24
AAC73120	588	369.244	69	55.6309

==> SRR23332740.se.tsv <==
b0001	239
b0002	115
b0003	68
b0004	126
b0005	10
b0006	28
b0007	19
b0008	660
b0009	85
b0010	41
SRR23332740 completed mapping pipeline successfully
