Starting /dee2/code/volunteer_pipeline.sh SRR23332724
    current disk space = 1464118148
    free memory = 58509268 
SRR23332724 SRAfilesize
b0128c8cdbe3917fa3110c35f26b479f  SRR23332724.sra
SRR23332724.sra file validated
SRR23332724 is paired end
SRR23332724 is conventional basespace
SRR23332724 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332724_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.3575	37.0	37.0	37.0	37.0	37.0
2	36.558	37.0	37.0	37.0	37.0	37.0
3	36.4925	37.0	37.0	37.0	37.0	37.0
4	36.434	37.0	37.0	37.0	37.0	37.0
5	36.484	37.0	37.0	37.0	37.0	37.0
6	36.3725	37.0	37.0	37.0	37.0	37.0
7	36.3895	37.0	37.0	37.0	37.0	37.0
8	36.4075	37.0	37.0	37.0	37.0	37.0
9	36.603	37.0	37.0	37.0	37.0	37.0
10	36.4615	37.0	37.0	37.0	37.0	37.0
11	36.4955	37.0	37.0	37.0	37.0	37.0
12	36.484	37.0	37.0	37.0	37.0	37.0
13	36.5235	37.0	37.0	37.0	37.0	37.0
14	36.3945	37.0	37.0	37.0	37.0	37.0
15	36.3815	37.0	37.0	37.0	37.0	37.0
16	36.523	37.0	37.0	37.0	37.0	37.0
17	36.347	37.0	37.0	37.0	37.0	37.0
18	36.574	37.0	37.0	37.0	37.0	37.0
19	36.3855	37.0	37.0	37.0	37.0	37.0
20	36.405	37.0	37.0	37.0	37.0	37.0
21	36.431	37.0	37.0	37.0	37.0	37.0
22	36.353	37.0	37.0	37.0	37.0	37.0
23	36.346	37.0	37.0	37.0	37.0	37.0
24	36.2965	37.0	37.0	37.0	37.0	37.0
25	36.456	37.0	37.0	37.0	37.0	37.0
26	36.269	37.0	37.0	37.0	37.0	37.0
27	36.4205	37.0	37.0	37.0	37.0	37.0
28	36.5015	37.0	37.0	37.0	37.0	37.0
29	36.2955	37.0	37.0	37.0	37.0	37.0
30	36.372	37.0	37.0	37.0	37.0	37.0
31	36.417	37.0	37.0	37.0	37.0	37.0
32	36.3855	37.0	37.0	37.0	37.0	37.0
33	36.396	37.0	37.0	37.0	37.0	37.0
34	36.2745	37.0	37.0	37.0	37.0	37.0
35	36.351	37.0	37.0	37.0	37.0	37.0
36	36.1105	37.0	37.0	37.0	37.0	37.0
37	36.298	37.0	37.0	37.0	37.0	37.0
38	36.3425	37.0	37.0	37.0	37.0	37.0
39	36.212	37.0	37.0	37.0	37.0	37.0
40	36.2205	37.0	37.0	37.0	37.0	37.0
41	36.141	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	2.0
25	2.0
26	5.0
27	4.0
28	7.0
29	12.0
30	29.0
31	30.0
32	52.0
33	93.0
34	101.0
35	259.0
36	1301.0
37	2102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.55	19.950000000000003	48.425000000000004	9.075
2	29.675	18.45	30.175	21.7
3	32.7	20.525	27.1	19.675
4	20.0	24.15	35.375	20.474999999999998
5	32.1	25.15	21.675	21.075
6	33.975	23.275000000000002	20.4	22.35
7	21.55	33.575	19.650000000000002	25.224999999999998
8	36.525	18.0	21.75	23.724999999999998
9	25.45	14.549999999999999	22.175	37.824999999999996
10	23.849999999999998	16.75	33.7	25.7
11	36.425000000000004	16.55	20.674999999999997	26.35
12	38.65	17.9	21.375	22.075
13	24.8	18.925	19.725	36.55
14	36.725	21.8	20.424999999999997	21.05
15	36.5	21.75	20.1	21.65
16	38.824999999999996	20.225	21.875	19.075
17	25.3	35.0	20.424999999999997	19.275000000000002
18	36.3	20.075000000000003	22.425	21.2
19	26.174999999999997	19.6	34.425	19.8
20	25.525	19.55	33.25	21.675
21	39.25	21.0	17.925	21.825
22	25.8	33.0	19.7	21.5
23	25.525	32.324999999999996	22.0	20.150000000000002
24	24.975	19.725	20.200000000000003	35.099999999999994
25	25.25	18.6	21.2	34.949999999999996
26	24.575	18.6	23.525	33.300000000000004
27	38.475	20.0	20.45	21.075
28	22.75	17.1	23.525	36.625
29	36.75	18.425	23.825	21.0
30	23.175	18.475	38.224999999999994	20.125
31	23.400000000000002	19.475	24.05	33.074999999999996
32	22.425	22.025	22.85	32.7
33	34.8	23.425	22.8	18.975
34	23.375	36.825	19.825	19.975
35	23.05	35.75	21.4	19.8
36	21.975	35.099999999999994	20.974999999999998	21.95
37	23.35	22.35	33.275	21.025
38	23.825	21.65	32.1	22.425
39	23.175	22.05	19.55	35.225
40	20.200000000000003	23.225	21.0	35.575
41	20.8	21.925	33.300000000000004	23.974999999999998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	1.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	1.5
24	2.0
25	2.0
26	4.0
27	6.0
28	14.5
29	23.0
30	21.5
31	20.0
32	20.0
33	22.5
34	25.0
35	29.0
36	33.0
37	33.0
38	62.5
39	92.0
40	138.5
41	185.0
42	185.0
43	207.0
44	229.0
45	310.5
46	392.0
47	392.0
48	413.5
49	435.0
50	439.5
51	444.0
52	463.0
53	482.0
54	482.0
55	437.0
56	392.0
57	345.0
58	298.0
59	298.0
60	518.0
61	738.0
62	422.0
63	106.0
64	106.0
65	84.0
66	62.0
67	43.5
68	25.0
69	25.0
70	13.5
71	2.0
72	1.5
73	1.0
74	2.0
75	3.0
76	3.0
77	1.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.5
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.85
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.99260476581759	46.85
2	15.201314708299096	18.5
3	3.820870994248151	6.9750000000000005
4	1.6844700082169268	4.1000000000000005
5	0.903861955628595	2.75
6	0.4108463434675432	1.5
7	0.2054231717337716	0.8750000000000001
8	0.3286770747740345	1.6
9	0.08216926869350863	0.44999999999999996
>10	0.3286770747740345	3.6999999999999997
>50	0.0	0.0
>100	0.0	0.0
>500	0.041084634346754315	12.7
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCT	508	12.7	No Hit
GCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACA	36	0.8999999999999999	No Hit
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTAC	29	0.7250000000000001	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCC	23	0.575	No Hit
GGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTA	18	0.44999999999999996	No Hit
TTCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	12	0.3	No Hit
GGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAACC	10	0.25	No Hit
TGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	10	0.25	No Hit
TGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACA	10	0.25	No Hit
GCAGGGCTTCCACGCGATCTCTTTTGGGTTTGACCTCTCTT	9	0.22499999999999998	No Hit
GGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAAC	9	0.22499999999999998	No Hit
TTTCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGA	8	0.2	No Hit
GGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTC	8	0.2	No Hit
GTCGCCCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAAT	8	0.2	No Hit
TGTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCT	8	0.2	No Hit
AAAAAACGCGCCCGAAGGCGCGTTGGCGATACACTCAATAT	8	0.2	No Hit
GATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGC	8	0.2	No Hit
ACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTAC	8	0.2	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCC	8	0.2	No Hit
TTCCGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTT	7	0.17500000000000002	No Hit
GCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACTAC	7	0.17500000000000002	No Hit
TTTATCACTGGAGGAAGGTGGGGATGACGTCAAGTCATCAT	7	0.17500000000000002	No Hit
GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAG	7	0.17500000000000002	No Hit
TATGAGGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCC	7	0.17500000000000002	No Hit
GTGTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACC	6	0.15	No Hit
TCTTTACATTCGCTTGCCAGCTGCGGACGGACACGCCACTA	6	0.15	No Hit
TTGCCAGCTGCGGACGGACACGCCACTAACAAACTAGCCTG	6	0.15	No Hit
TTTCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTCC	6	0.15	No Hit
TCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGT	6	0.15	No Hit
AGGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGA	6	0.15	No Hit
ACTTCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGA	6	0.15	No Hit
TCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACA	6	0.15	No Hit
CTTCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAA	6	0.15	No Hit
TTACATTCGCTTGCCAGCTGCGGACGGACACGCCACTAACA	6	0.15	No Hit
GGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGC	5	0.125	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACGAGAGGACCGGGA	5	0.125	No Hit
TTCATGGAGTCGAGTTGCTAGTAATCGTGGATCAGAATGCC	5	0.125	No Hit
AAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAA	5	0.125	No Hit
TATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAG	5	0.125	No Hit
TTCCGACTTCATGGATCAGAATGCCACGGTGAATACGTTCC	5	0.125	No Hit
TCCGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTA	5	0.125	No Hit
GCCTGATTAAGTTTTAACGCTTCAACCCCAGGCAGGGCTTC	5	0.125	No Hit
TGTGACGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACC	5	0.125	No Hit
TAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTC	5	0.125	No Hit
GAAAACCAGGCAAATCCGGAAAATCAAGGCTGAGGCGTGAT	5	0.125	No Hit
TGGTGGAGCTGGCGGGAGTTGAGCCCGCGTCCGAAATTCCT	5	0.125	No Hit
GGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACAT	5	0.125	No Hit
GTTTTAACGCTTCAACCCCAGGCAGGGCTTCCACGCGATCT	5	0.125	No Hit
AGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCCC	5	0.125	No Hit
CTACTTCTTTTGCAACCCACTTTGTGATTCATGACTGGGGT	5	0.125	No Hit
TTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCAC	5	0.125	No Hit
GAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACAGA	5	0.125	No Hit
TTCCTACATCCTCGGTACTACATGCTTAGTCAGTCTTTACA	5	0.125	No Hit
GTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTT	5	0.125	No Hit
TACTAGCGATTCCCGGGCCTTGTACACACCGCCCGTCACAC	5	0.125	No Hit
GATCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.1	0.0	0.0	0.0	0.0
4	0.2	0.0	0.0	0.0	0.0
5	0.25	0.0	0.0	0.0	0.0
6	0.25	0.0	0.0	0.0	0.0
7	0.25	0.0	0.0	0.0	0.0
8	0.25	0.0	0.0	0.0	0.0
9	0.275	0.0	0.0	0.0	0.0
10	0.3	0.0	0.0	0.0	0.0
11	0.3	0.0	0.0	0.0	0.0
12	0.3	0.0	0.0	0.0	0.0
13	0.35	0.0	0.0	0.0	0.0
14	0.4	0.0	0.0	0.0	0.0
15	0.4	0.0	0.0	0.0	0.0
16	0.425	0.0	0.0	0.0	0.0
17	0.425	0.0	0.0	0.0	0.0
18	0.425	0.0	0.0	0.0	0.0
19	0.475	0.0	0.0	0.0	0.0
20	0.525	0.0	0.0	0.0	0.0
21	0.55	0.0	0.0	0.0	0.0
22	0.625	0.0	0.0	0.0	0.0
23	0.7	0.0	0.0	0.0	0.0
24	0.725	0.0	0.0	0.0	0.0
25	0.8	0.0	0.0	0.0	0.0
26	0.85	0.0	0.0	0.0	0.0
27	0.9	0.0	0.0	0.0	0.0
28	0.95	0.0	0.0	0.0	0.0
29	0.975	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTGGA	35	6.175269E-7	35.000004	1
GGTGGAG	40	1.7565599E-6	30.625	2
GCGGGAG	50	2.8585237E-7	27.999998	12
GTTGAAC	50	2.8585237E-7	27.999998	18
GAAATTC	50	2.8585237E-7	27.999998	33
AAATTCC	50	2.8585237E-7	27.999998	34
AATTCCT	50	2.8585237E-7	27.999998	35
GTCCGAA	50	2.8585237E-7	27.999998	29
GCTGGCG	50	2.8585237E-7	27.999998	8
AACCCGC	50	2.8585237E-7	27.999998	22
ACCCGCG	50	2.8585237E-7	27.999998	23
GGGAGTT	50	2.8585237E-7	27.999998	14
GAGTTGA	50	2.8585237E-7	27.999998	16
CTGGCGG	50	2.8585237E-7	27.999998	9
CCCGCGT	50	2.8585237E-7	27.999998	24
GAACCCG	50	2.8585237E-7	27.999998	21
CGGGAGT	50	2.8585237E-7	27.999998	13
GCGTCCG	50	2.8585237E-7	27.999998	27
CGAAATT	50	2.8585237E-7	27.999998	32
AGTTGAA	50	2.8585237E-7	27.999998	17
>>END_MODULE
SRR23332724 read2 length is 39-50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332724_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	39-50
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.353	11.0	11.0	37.0	11.0	37.0
2	22.2455	25.0	11.0	37.0	11.0	37.0
3	24.747	25.0	11.0	37.0	11.0	37.0
4	36.1395	37.0	37.0	37.0	37.0	37.0
5	36.1435	37.0	37.0	37.0	37.0	37.0
6	36.014	37.0	37.0	37.0	37.0	37.0
7	35.7045	37.0	37.0	37.0	37.0	37.0
8	35.9375	37.0	37.0	37.0	37.0	37.0
9	35.8545	37.0	37.0	37.0	37.0	37.0
10	35.9665	37.0	37.0	37.0	37.0	37.0
11	36.0265	37.0	37.0	37.0	37.0	37.0
12	35.9935	37.0	37.0	37.0	37.0	37.0
13	35.989	37.0	37.0	37.0	37.0	37.0
14	36.0795	37.0	37.0	37.0	37.0	37.0
15	35.833	37.0	37.0	37.0	37.0	37.0
16	36.013	37.0	37.0	37.0	37.0	37.0
17	36.0465	37.0	37.0	37.0	37.0	37.0
18	36.279	37.0	37.0	37.0	37.0	37.0
19	36.0515	37.0	37.0	37.0	37.0	37.0
20	36.0545	37.0	37.0	37.0	37.0	37.0
21	36.0265	37.0	37.0	37.0	37.0	37.0
22	36.2765	37.0	37.0	37.0	37.0	37.0
23	36.086	37.0	37.0	37.0	37.0	37.0
24	36.1265	37.0	37.0	37.0	37.0	37.0
25	36.023	37.0	37.0	37.0	37.0	37.0
26	36.07775	37.0	37.0	37.0	37.0	37.0
27	35.996	37.0	37.0	37.0	37.0	37.0
28	36.126	37.0	37.0	37.0	37.0	37.0
29	36.047	37.0	37.0	37.0	37.0	37.0
30	36.0965	37.0	37.0	37.0	37.0	37.0
31	36.1485	37.0	37.0	37.0	37.0	37.0
32	35.974	37.0	37.0	37.0	37.0	37.0
33	36.052	37.0	37.0	37.0	37.0	37.0
34	36.2	37.0	37.0	37.0	37.0	37.0
35	36.045	37.0	37.0	37.0	37.0	37.0
36	36.25875	37.0	37.0	37.0	37.0	37.0
37	36.01	37.0	37.0	37.0	37.0	37.0
38	35.9895	37.0	37.0	37.0	37.0	37.0
39	36.016	37.0	37.0	37.0	37.0	37.0
40	36.10277569392348	37.0	37.0	37.0	37.0	37.0
41	35.7962962962963	37.0	37.0	37.0	37.0	37.0
42	36.048072108162245	37.0	37.0	37.0	37.0	37.0
43	35.93931795386158	37.0	37.0	37.0	37.0	37.0
44	36.04877432398281	37.0	37.0	37.0	37.0	37.0
45	35.84963196635121	37.0	37.0	37.0	37.0	37.0
46	35.914346327234156	37.0	37.0	37.0	37.0	37.0
47	36.013444474321055	37.0	37.0	37.0	37.0	37.0
48	36.05366385181149	37.0	37.0	37.0	37.0	37.0
49	36.147595861229455	37.0	37.0	37.0	37.0	37.0
50	35.82805755395683	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	0.0
16	0.0
17	0.0
18	1.0
19	1.0
20	4.0
21	5.0
22	10.0
23	10.0
24	5.0
25	9.0
26	15.0
27	14.0
28	27.0
29	31.0
30	52.0
31	56.0
32	98.0
33	150.0
34	372.0
35	1755.0
36	1196.0
37	187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	40.675	29.875	29.45
2	33.050000000000004	40.075	11.85	15.024999999999999
3	28.125	41.525	15.575	14.774999999999999
4	80.5	7.35	5.800000000000001	6.35
5	64.725	16.7	10.9	7.675
6	59.275	21.45	8.475000000000001	10.8
7	35.449999999999996	42.699999999999996	10.025	11.825
8	42.925000000000004	19.775000000000002	15.2	22.1
9	20.4	38.45	27.575	13.575000000000001
10	41.925000000000004	22.375	16.7	19.0
11	29.775000000000002	39.050000000000004	15.85	15.325
12	22.575	26.75	27.35	23.325000000000003
13	37.05	15.075	29.299999999999997	18.575
14	34.8	16.975	24.45	23.775
15	26.474999999999998	18.3	44.324999999999996	10.9
16	44.074999999999996	22.275	22.575	11.075
17	27.500000000000004	33.6	17.974999999999998	20.925
18	15.8	39.425	27.825	16.950000000000003
19	20.525	19.55	34.1	25.825
20	16.525000000000002	23.075000000000003	31.225	29.175
21	18.825	42.225	12.5	26.450000000000003
22	38.800000000000004	29.975	13.750000000000002	17.474999999999998
23	21.525	30.65	19.675	28.15
24	23.0	22.175	13.475000000000001	41.349999999999994
25	39.4	17.875	12.5	30.225
26	33.25831457864466	20.7551887971993	13.653413353338333	32.3330832708177
27	38.574999999999996	25.025	20.7	15.7
28	33.04152076038019	25.63781890945473	27.5887943971986	13.731865932966484
29	25.0	15.65	44.775	14.575
30	20.210105052526263	15.232616308154077	50.32516258129065	14.232116058029016
31	17.224999999999998	22.1	40.875	19.8
32	32.266133066533264	14.132066033016507	20.96048024012006	32.641320660330166
33	21.375	14.149999999999999	15.2	49.275000000000006
34	25.737868934467233	18.05902951475738	23.261630815407706	32.94147073536769
35	12.45	33.725	22.900000000000002	30.925000000000004
36	12.528132033008252	39.13478369592398	30.282570642660666	18.0545136284071
37	17.05	45.324999999999996	13.4	24.224999999999998
38	20.45	20.525	11.35	47.675
39	35.375	18.35	12.075	34.2
40	23.95598899724931	20.230057514378593	18.879719929982496	36.93423355838959
41	16.766766766766768	40.14014014014014	27.57757757757758	15.515515515515515
42	20.655983975963945	27.616424636955433	29.619429143715575	22.108162243365047
43	31.569709127382144	24.09729187562688	15.195586760280843	29.13741223671013
44	39.297447561283796	15.061915592620673	18.953752843062926	26.686884003032603
45	18.27024185068349	16.27234490010515	34.38485804416404	31.07255520504732
46	30.89366215857863	18.987006099177936	32.98859718907451	17.13073455316892
47	29.72027972027972	15.30392684238838	27.218934911242602	27.756858526089296
48	27.839825660582946	25.08853173522201	25.68782348134023	21.38381912285481
49	29.43683409436834	12.998477929984778	41.61339421613394	15.951293759512938
50	30.755395683453234	13.381294964028779	28.633093525179852	27.230215827338128
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	2.0
6	1.0
7	1.0
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.0
20	2.0
21	1.0
22	0.0
23	2.0
24	4.0
25	3.0
26	2.0
27	3.5
28	7.0
29	15.0
30	22.5
31	31.0
32	41.0
33	49.5
34	61.0
35	80.5
36	90.0
37	79.5
38	73.0
39	82.0
40	93.0
41	92.5
42	92.0
43	104.0
44	117.5
45	125.5
46	132.0
47	138.0
48	143.0
49	182.0
50	229.0
51	375.5
52	528.5
53	699.5
54	881.0
55	858.0
56	760.5
57	604.0
58	458.5
59	300.0
60	170.5
61	119.5
62	73.0
63	53.5
64	32.5
65	22.0
66	16.5
67	12.0
68	7.5
69	8.5
70	8.5
71	4.5
72	1.5
73	1.0
74	1.0
75	1.5
76	2.0
77	1.5
78	1.5
79	2.0
80	1.5
81	0.5
82	0.0
83	0.5
84	1.0
85	1.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.025
27	0.0
28	0.05
29	0.0
30	0.05
31	0.0
32	0.05
33	0.0
34	0.05
35	0.0
36	0.025
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.026888948642108095
48	0.0
49	0.030432136335970784
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
39	1.0
40	3.0
41	2.0
42	6.0
43	31.0
44	153.0
45	33.0
46	52.0
47	48.0
48	385.0
49	506.0
50	2780.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.45
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.64185277088504	52.37500000000001
2	5.128205128205128	6.2
3	1.8610421836228286	3.375
4	1.0339123242349049	2.5
5	1.3234077750206783	4.0
6	0.8684863523573202	3.15
7	0.6617038875103392	2.8000000000000003
8	0.45492142266335817	2.1999999999999997
9	0.3308519437551696	1.7999999999999998
>10	1.5715467328370554	16.150000000000002
>50	0.12406947890818859	5.45
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	87	2.175	No Hit
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	68	1.7000000000000002	No Hit
AAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	63	1.575	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	40	1.0	No Hit
AAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	39	0.975	No Hit
CAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	34	0.8500000000000001	No Hit
CGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	26	0.65	No Hit
TGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	26	0.65	No Hit
TAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	25	0.625	No Hit
CAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	23	0.575	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	23	0.575	No Hit
AATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	20	0.5	No Hit
AACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	20	0.5	No Hit
CGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	19	0.475	No Hit
TGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	18	0.44999999999999996	No Hit
CTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	18	0.44999999999999996	No Hit
AGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
TGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
CCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
AGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	15	0.375	No Hit
TGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	14	0.35000000000000003	No Hit
CCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
TAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	14	0.35000000000000003	No Hit
ATAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	13	0.325	No Hit
AATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
CCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
CAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	13	0.325	No Hit
ACCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
CGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
TATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
TGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
ACAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
TGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	12	0.3	No Hit
CGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
CGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
AGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTA	10	0.25	No Hit
TGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
CATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
TACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
CGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	9	0.22499999999999998	No Hit
TGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
TAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
CAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
AAAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	8	0.2	No Hit
CCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
TCGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
AAAGTGATTGCACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCA	8	0.2	No Hit
TGAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
CAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
AAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
AATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
AGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
CTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
TCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
AGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
ACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
ATAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
AAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
ACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
TAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
AGACACGGTACTAATGAACCGTGAGGCTTAACCTTAGTCTGGCG	7	0.17500000000000002	No Hit
ACAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
ATAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
AACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AAAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAT	6	0.15	No Hit
CGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TGTGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TGTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
AGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
AAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
ACAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
CATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CGAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
AGCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
AACGAATGGCGTAATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGA	6	0.15	No Hit
TATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
AAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AAAGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCT	5	0.125	No Hit
AGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
AAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
AATGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCT	5	0.125	No Hit
TTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CCTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CCAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TTTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
ACTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AAAGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTA	5	0.125	No Hit
AACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
ATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CACGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AACGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TTTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.05	0.0	0.0	0.0	0.0
9	0.05	0.0	0.0	0.0	0.0
10	0.1	0.0	0.0	0.0	0.0
11	0.1	0.0	0.0	0.0	0.0
12	0.15	0.0	0.0	0.0	0.0
13	0.225	0.0	0.0	0.0	0.0
14	0.275	0.0	0.0	0.0	0.0
15	0.275	0.0	0.0	0.0	0.0
16	0.275	0.0	0.0	0.0	0.0
17	0.3	0.0	0.0	0.0	0.0
18	0.3	0.0	0.0	0.0	0.0
19	0.3	0.0	0.0	0.0	0.0
20	0.3	0.0	0.0	0.0	0.0
21	0.325	0.0	0.0	0.0	0.0
22	0.375	0.0	0.0	0.0	0.0
23	0.4	0.0	0.0	0.0	0.0
24	0.45	0.0	0.0	0.0	0.0
25	0.475	0.0	0.0	0.0	0.0
26	0.5	0.0	0.0	0.0	0.0
27	0.575	0.0	0.0	0.0	0.0
28	0.65	0.0	0.0	0.0	0.0
29	0.675	0.0	0.0	0.0	0.0
30	0.7	0.0	0.0	0.0	0.0
31	0.85	0.0	0.0	0.0	0.0
32	0.875	0.0	0.0	0.0	0.0
33	0.925	0.0	0.0	0.0	0.0
34	0.975	0.0	0.0	0.0	0.0
35	1.0	0.0	0.0	0.0	0.0
36	1.0	0.0	0.0	0.0	0.0
37	1.0	0.0	0.0	0.0	0.0
38	1.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCATG	45	7.2759576E-12	62.61818	44
GGTGCAT	50	9.094947E-11	48.43125	43
AGGTGCA	50	2.0372681E-10	44.921738	42
AAGGTGC	50	2.3646862E-10	44.28	41
AGGGGCT	25	4.204621E-5	43.05	3
AAGGGGC	20	7.7415985E-4	43.05	2
CAAGGTG	50	3.6743586E-10	42.460274	40
CCAAGGT	50	3.6743586E-10	42.460274	39
GTAGTGC	30	8.5068546E-4	41.745453	44
AGGGAGA	75	0.0	40.18	3
TCGACGG	50	9.549694E-10	38.745	20
GAAACCC	50	9.549694E-10	38.745	34
TCTGGAT	50	9.549694E-10	38.745	13
TGATTCT	50	9.549694E-10	38.745	9
GACGGGA	50	9.549694E-10	38.745	22
GGATTTG	50	9.549694E-10	38.745	26
TTCGACG	50	9.549694E-10	38.745	19
TTCTGGA	50	9.549694E-10	38.745	12
GGATTCG	50	9.549694E-10	38.745	16
CTGATTC	50	9.549694E-10	38.745	8
>>END_MODULE
Read 302302 spots for SRR23332724.sra
Written 302302 spots for SRR23332724.sra
Read 302302 spots for SRR23332724.sra
Written 302302 spots for SRR23332724.sra
Read 302302 spots for SRR23332724.sra
Written 302302 spots for SRR23332724.sra
Read 302302 spots for SRR23332724.sra
Written 302302 spots for SRR23332724.sra
Read 302302 spots for SRR23332724.sra
Written 302302 spots for SRR23332724.sra
Read 302302 spots for SRR23332724.sra
Written 302302 spots for SRR23332724.sra
Read 302302 spots for SRR23332724.sra
Written 302302 spots for SRR23332724.sra
Read 302302 spots for SRR23332724.sra
Written 302302 spots for SRR23332724.sra
SRR ids: ['SRR23332724.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_x69yyzsn
SRR23332724.sra spots: 2418416
blocks: [[1, 302302], [302303, 604604], [604605, 906906], [906907, 1209208], [1209209, 1511510], [1511511, 1813812], [1813813, 2116114], [2116115, 2418416]]
SRR23332724 file size 293048
SRR23332724 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR23332724 SRR23332724_1.fastq SRR23332724_2.fastq
Input file:	SRR23332724_1.fastq
Paired file:	SRR23332724_2.fastq
trimmed:	SRR23332724-trimmed-pair1.fastq, SRR23332724-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 03:18:49 2026 >> started

Wed Mar 25 03:18:51 2026 >> done (1.429s)
2418416 read pairs processed; of these:
    489 ( 0.02%) short read pairs filtered out after trimming by size control
     78 ( 0.00%) empty read pairs filtered out after trimming by size control
2417849 (99.98%) read pairs available; of these:
  18413 ( 0.76%) trimmed read pairs available after processing
2399436 (99.24%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    191	  0.01%
 19	    332	  0.01%
 20	    595	  0.02%
 21	    280	  0.01%
 22	    190	  0.01%
 23	    171	  0.01%
 24	    290	  0.01%
 25	    464	  0.02%
 26	    284	  0.01%
 27	    340	  0.01%
 28	    303	  0.01%
 29	    616	  0.03%
 30	    446	  0.02%
 31	    493	  0.02%
 32	    596	  0.02%
 33	    751	  0.03%
 34	    923	  0.04%
 35	    854	  0.04%
 36	    877	  0.04%
 37	    774	  0.03%
 38	   1261	  0.05%
 39	   1840	  0.08%
 40	   3464	  0.14%
 41	   2872	  0.12%
 42	 106223	  4.39%
 43	  50870	  2.10%
 44	 222154	  9.19%
 45	2019395	 83.52%
2417849 reads passed initial QC


criterion=sequence-density
sequence-density=1.34
sequence-density-rank=1
fanout-score=1.94
fanout-score-rank=28
prefix-density=1.24
prefix-fanout=1.9
sequence=GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=39
fanout-score=33.45
fanout-score-rank=1
prefix-density=0.24
prefix-fanout=1.2
sequence=TTTTTTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACAGAATCAGGCCATTACTACCCTGTCCGCCATGGCTTCTCGCCAGCCTCCCAGCCATTGTGACCTTTGATTCAGCGTCTGATAGGGACACATTTCTTTTGAGCGTCCGGCGATGCCGGCCTGATAACCACGTTGATGTGCCCGTTCCAGGCGATCTCGTTTTTGTCTCTTCATGCCTCGTTTCCCTCATACTGGTTTCTGG


criterion=sequence-density
sequence-density=15.05
sequence-density-rank=1
fanout-score=22.05
fanout-score-rank=4
prefix-density=11.59
prefix-fanout=22.0
sequence=GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGGACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCTCACGGTT


criterion=fanout-score
sequence-density=0.68
sequence-density-rank=11
fanout-score=167.59
fanout-score-rank=1
prefix-density=10.05
prefix-fanout=11.3
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTAGTCTGGCG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGA -y GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGGACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCTCACGGTT -o SRR23332724 SRR23332724_1.fastq SRR23332724_2.fastq
Input file:	SRR23332724_1.fastq
Paired file:	SRR23332724_2.fastq
trimmed:	SRR23332724-trimmed-pair1.fastq, SRR23332724-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAG
-- paired 3' end adapter sequence (-y):	GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 03:18:57 2026 >> started

Wed Mar 25 03:18:58 2026 >> done (1.334s)
1880549 read pairs processed; of these:
  15469 ( 0.82%) short read pairs filtered out after trimming by size control
   3626 ( 0.19%) empty read pairs filtered out after trimming by size control
1861454 (98.98%) read pairs available; of these:
    681 ( 0.04%) trimmed read pairs available after processing
1860773 (99.96%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    149	  0.01%
 19	    256	  0.01%
 20	    435	  0.02%
 21	    210	  0.01%
 22	    150	  0.01%
 23	    136	  0.01%
 24	    215	  0.01%
 25	    345	  0.02%
 26	    218	  0.01%
 27	    277	  0.01%
 28	    238	  0.01%
 29	    478	  0.03%
 30	    356	  0.02%
 31	    394	  0.02%
 32	    454	  0.02%
 33	    549	  0.03%
 34	    725	  0.04%
 35	    669	  0.04%
 36	    692	  0.04%
 37	    606	  0.03%
 38	    963	  0.05%
 39	   1425	  0.08%
 40	   2688	  0.14%
 41	   2279	  0.12%
 42	  82479	  4.43%
 43	  39554	  2.12%
 44	 164191	  8.82%
 45	1560323	 83.82%


criterion=sequence-density
sequence-density=1.22
sequence-density-rank=1
fanout-score=2.51
fanout-score-rank=21
prefix-density=1.23
prefix-fanout=2.5
sequence=ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=40
fanout-score=33.25
fanout-score-rank=1
prefix-density=0.24
prefix-fanout=1.2
sequence=TTTTTTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACAGAATCAGGCCATTACTACCCTGTCCGCCATGGCTTCTCGCCAGCCTCCCAGCCATTGTGACCTTTGATTCAGCGTCTGATAGGGACACATTTCTTTTGAGCGTCCGGCGATGCCGGCCTGATAACCACGTTGATGTGCCCGTTCCAGGCGATCTCGTTTTTGTCTCTTCATGCCTCGTTTCCCTCATACTGGTTTCTGGTGG


criterion=sequence-density
sequence-density=14.61
sequence-density-rank=1
fanout-score=17.59
fanout-score-rank=8
prefix-density=12.46
prefix-fanout=17.6
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCA


criterion=fanout-score
sequence-density=0.69
sequence-density-rank=11
fanout-score=167.34
fanout-score-rank=1
prefix-density=10.11
prefix-fanout=11.3
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTAGTCTGGCGA
SRR23332724 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 25 03:19:15
                             Started mapping on |	Mar 25 03:19:15
                                    Finished on |	Mar 25 03:20:50
       Mapping speed, Million of reads per hour |	90.90

                          Number of input reads |	2398754
                      Average input read length |	90
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1297591
                        Uniquely mapped reads % |	54.09%
                          Average mapped length |	87.74
                       Number of splices: Total |	76662
            Number of splices: Annotated (sjdb) |	66
                       Number of splices: GT/AG |	69738
                       Number of splices: GC/AG |	1867
                       Number of splices: AT/AC |	265
               Number of splices: Non-canonical |	4792
                      Mismatch rate per base, % |	0.28%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.26
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	502841
             % of reads mapped to multiple loci |	20.96%
        Number of reads mapped to too many loci |	444341
             % of reads mapped to too many loci |	18.52%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.02%
                     % of reads unmapped: other |	0.40%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	598324	598324	598324
N_multimapping	502841	502841	502841
N_noFeature	53840	1171117	100940
N_ambiguous	128594	17170	37975
UnstrandedReadsAssigned:1115157 PositiveStrandReadsAssigned:109304 NegativeStrandReadsAssigned:1158676
Dataset is classified negative stranded
MeadianReadLen=41 20thPercentileLength=41 echo kmer=37
SRR23332724 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR23332724-trimmed-pair1.fastq
                             SRR23332724-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,398,754 reads, 485,405 reads pseudoaligned
[quant] estimated average fragment length: 202.91
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 318 rounds

  4323 SRR23332724.ke.tsv
  4497 SRR23332724.se.tsv
  8820 total
==> SRR23332724.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	14.8408	144	2902.21
AAC73113	2463	2261.09	74	9.789
AAC73114	933	731.104	25	10.2279
AAC73115	1287	1085.09	50	13.7825
AAC73116	297	120.865	3	7.42412
AAC73117	777	575.191	13	6.76014
AAC73118	1431	1229.09	28	6.81395
AAC73119	954	752.104	218	86.6968
AAC73120	588	387.627	26	20.0625

==> SRR23332724.se.tsv <==
b0001	206
b0002	73
b0003	22
b0004	42
b0005	3
b0006	17
b0007	30
b0008	216
b0009	27
b0010	7
SRR23332724 completed mapping pipeline successfully
