Starting /dee2/code/volunteer_pipeline.sh SRR23332709
    current disk space = 1463270300
    free memory = 58909824 
SRR23332709 SRAfilesize
8b11ebd59993c5d5faefc4e23210a393  SRR23332709.sra
SRR23332709.sra file validated
SRR23332709 is paired end
SRR23332709 is conventional basespace
SRR23332709 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332709_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.513	37.0	37.0	37.0	37.0	37.0
2	36.5775	37.0	37.0	37.0	37.0	37.0
3	36.488	37.0	37.0	37.0	37.0	37.0
4	36.433	37.0	37.0	37.0	37.0	37.0
5	36.4475	37.0	37.0	37.0	37.0	37.0
6	36.314	37.0	37.0	37.0	37.0	37.0
7	36.4125	37.0	37.0	37.0	37.0	37.0
8	36.4655	37.0	37.0	37.0	37.0	37.0
9	36.528	37.0	37.0	37.0	37.0	37.0
10	36.5615	37.0	37.0	37.0	37.0	37.0
11	36.4595	37.0	37.0	37.0	37.0	37.0
12	36.5455	37.0	37.0	37.0	37.0	37.0
13	36.524	37.0	37.0	37.0	37.0	37.0
14	36.5315	37.0	37.0	37.0	37.0	37.0
15	36.573	37.0	37.0	37.0	37.0	37.0
16	36.559	37.0	37.0	37.0	37.0	37.0
17	36.3685	37.0	37.0	37.0	37.0	37.0
18	36.3495	37.0	37.0	37.0	37.0	37.0
19	36.3725	37.0	37.0	37.0	37.0	37.0
20	36.4085	37.0	37.0	37.0	37.0	37.0
21	36.45	37.0	37.0	37.0	37.0	37.0
22	36.405	37.0	37.0	37.0	37.0	37.0
23	36.4125	37.0	37.0	37.0	37.0	37.0
24	36.384	37.0	37.0	37.0	37.0	37.0
25	36.473	37.0	37.0	37.0	37.0	37.0
26	36.362	37.0	37.0	37.0	37.0	37.0
27	36.3415	37.0	37.0	37.0	37.0	37.0
28	36.3865	37.0	37.0	37.0	37.0	37.0
29	36.43	37.0	37.0	37.0	37.0	37.0
30	36.4475	37.0	37.0	37.0	37.0	37.0
31	36.472	37.0	37.0	37.0	37.0	37.0
32	36.319	37.0	37.0	37.0	37.0	37.0
33	36.5305	37.0	37.0	37.0	37.0	37.0
34	36.428	37.0	37.0	37.0	37.0	37.0
35	36.421	37.0	37.0	37.0	37.0	37.0
36	36.359	37.0	37.0	37.0	37.0	37.0
37	36.2695	37.0	37.0	37.0	37.0	37.0
38	36.2725	37.0	37.0	37.0	37.0	37.0
39	36.3355	37.0	37.0	37.0	37.0	37.0
40	36.2905	37.0	37.0	37.0	37.0	37.0
41	36.401	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	1.0
24	1.0
25	4.0
26	4.0
27	9.0
28	10.0
29	19.0
30	24.0
31	38.0
32	41.0
33	78.0
34	79.0
35	189.0
36	1230.0
37	2272.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.425	18.475	49.725	8.375
2	38.5	17.025000000000002	25.324999999999996	19.15
3	39.975	18.224999999999998	22.525000000000002	19.275000000000002
4	19.1	18.625	42.75	19.525000000000002
5	38.875	19.675	21.825	19.625
6	41.075	19.7	18.075	21.15
7	19.975	39.775	17.724999999999998	22.525000000000002
8	42.35	16.225	18.475	22.95
9	23.95	13.075000000000001	19.325	43.65
10	23.400000000000002	13.8	38.975	23.825
11	43.65	15.325	18.2	22.825
12	42.875	16.5	19.35	21.275
13	23.9	17.825	17.95	40.325
14	42.65	19.15	20.375	17.825
15	43.5	19.1	20.325	17.075000000000003
16	44.7	19.8	19.400000000000002	16.1
17	22.05	41.25	21.099999999999998	15.6
18	43.675000000000004	17.349999999999998	21.8	17.175
19	23.075000000000003	16.925	41.475	18.525
20	22.45	18.95	38.1	20.5
21	43.9	19.125	17.75	19.225
22	22.425	40.0	17.974999999999998	19.6
23	22.375	39.975	16.875	20.775
24	24.125	18.4	16.45	41.025
25	26.575	15.7	18.525	39.2
26	23.35	15.75	19.475	41.425
27	43.3	17.8	17.875	21.025
28	22.025	15.75	20.0	42.225
29	43.75	16.150000000000002	20.1	20.0
30	20.974999999999998	18.15	42.05	18.825
31	21.0	18.35	19.825	40.825
32	21.2	19.85	19.400000000000002	39.550000000000004
33	42.575	19.35	19.45	18.625
34	20.7	41.0	18.525	19.775000000000002
35	19.400000000000002	40.575	20.125	19.900000000000002
36	20.549999999999997	41.349999999999994	17.849999999999998	20.25
37	21.8	18.925	39.300000000000004	19.975
38	22.325	18.6	37.925	21.15
39	22.5	18.825	17.25	41.425
40	19.375	21.025	17.525	42.075
41	20.674999999999997	18.05	38.224999999999994	23.05
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	1.0
20	1.0
21	0.5
22	0.0
23	2.0
24	4.0
25	4.0
26	2.0
27	0.0
28	1.5
29	3.0
30	5.5
31	8.0
32	8.0
33	10.0
34	12.0
35	16.5
36	21.0
37	21.0
38	34.5
39	48.0
40	88.5
41	129.0
42	129.0
43	162.0
44	195.0
45	226.0
46	257.0
47	257.0
48	331.0
49	405.0
50	415.0
51	425.0
52	475.5
53	526.0
54	526.0
55	451.5
56	377.0
57	338.5
58	300.0
59	300.0
60	704.0
61	1108.0
62	602.0
63	96.0
64	96.0
65	73.0
66	50.0
67	34.5
68	19.0
69	19.0
70	16.0
71	13.0
72	6.5
73	0.0
74	1.0
75	2.0
76	2.0
77	1.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.33443552196505	40.400000000000006
2	15.068493150684931	15.950000000000001
3	4.10958904109589	6.525
4	2.031176192725555	4.3
5	0.6140765233821445	1.625
6	0.47236655644780345	1.5
7	0.23618327822390173	0.8750000000000001
8	0.28341993386868214	1.2
9	0.1889466225791214	0.8999999999999999
>10	0.6140765233821445	5.949999999999999
>50	0.0	0.0
>100	0.0	0.0
>500	0.04723665564478035	20.775
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCT	831	20.775	No Hit
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTAC	37	0.9249999999999999	No Hit
GGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTA	29	0.7250000000000001	No Hit
GGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAAC	26	0.65	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCC	23	0.575	No Hit
AAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGAG	19	0.475	No Hit
TGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	18	0.44999999999999996	No Hit
AAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTC	17	0.42500000000000004	No Hit
AGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAA	13	0.325	No Hit
TTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCAC	12	0.3	No Hit
GCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACA	12	0.3	No Hit
GAGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGG	11	0.27499999999999997	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCC	11	0.27499999999999997	No Hit
TCCTTCTTTGGTTACAGCTGTAGATCGGAAGAGCACACGTC	10	0.25	No Hit
GGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAACC	9	0.22499999999999998	No Hit
TTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACT	9	0.22499999999999998	No Hit
TTTCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTCC	9	0.22499999999999998	No Hit
GTCTTCAACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATG	9	0.22499999999999998	No Hit
GCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACTAC	8	0.2	No Hit
TTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGA	8	0.2	No Hit
AAAAAACGCGCCCGAAGGCGCGTTGGCGATACACTCAATAT	8	0.2	No Hit
AGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGT	8	0.2	No Hit
AAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCG	8	0.2	No Hit
AGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCCA	8	0.2	No Hit
TGTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCT	7	0.17500000000000002	No Hit
GGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACAT	7	0.17500000000000002	No Hit
TTCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	7	0.17500000000000002	No Hit
ATTCCGACTTCATGACTGGGGTGAAGTCGTAACAAGGTAAC	7	0.17500000000000002	No Hit
AAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTT	7	0.17500000000000002	No Hit
GACACGCCACTAACAAACTAGCCTGATTAAGTTTTAACGCT	6	0.15	No Hit
AAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTT	6	0.15	No Hit
GACCTCTCTTGATCCCCGTCCTAAGAGCGGAGGCTAGGGAG	6	0.15	No Hit
GGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAA	6	0.15	No Hit
TCATGGAGTCGAGTTGCTAGTAATCGTGGATCAGAATGCCA	6	0.15	No Hit
AGGTTAAGCCTCACGGTTCATTAGTACGAGAGGACCGGGAT	6	0.15	No Hit
AGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCCC	6	0.15	No Hit
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCT	6	0.15	TruSeq Adapter, Index 8 (97% over 37bp)
TTATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGAA	6	0.15	No Hit
TTCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAG	6	0.15	No Hit
TTTCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGA	5	0.125	No Hit
TTTGGGTTTGACCTCTCTTGATCCCCGTCCTAAGAGCGGAG	5	0.125	No Hit
GGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTC	5	0.125	No Hit
GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAG	5	0.125	No Hit
GTCGCCCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAAT	5	0.125	No Hit
GTATTCACCGTGGGAGTGGGTTGCAAAAGAAGTAGGTAGCT	5	0.125	No Hit
ATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCA	5	0.125	No Hit
TCAACCCCAGGCAGGGCTTCCACGCGATCTCTTTTGGGTTT	5	0.125	No Hit
GTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTC	5	0.125	No Hit
CGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTCCTGA	5	0.125	No Hit
TATCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGA	5	0.125	No Hit
ACTAGCCTGATTAAGTTTTAACGCTTCAACCCCAGGCAGGG	5	0.125	No Hit
ATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.15	0.0	0.0	0.0	0.0
5	0.15	0.0	0.0	0.0	0.0
6	0.15	0.0	0.0	0.0	0.0
7	0.15	0.0	0.0	0.0	0.0
8	0.15	0.0	0.0	0.0	0.0
9	0.15	0.0	0.0	0.0	0.0
10	0.15	0.0	0.0	0.0	0.0
11	0.15	0.0	0.0	0.0	0.0
12	0.15	0.0	0.0	0.0	0.0
13	0.15	0.0	0.0	0.0	0.0
14	0.15	0.0	0.0	0.0	0.0
15	0.15	0.0	0.0	0.0	0.0
16	0.15	0.0	0.0	0.0	0.0
17	0.15	0.0	0.0	0.0	0.0
18	0.15	0.0	0.0	0.0	0.0
19	0.15	0.0	0.0	0.0	0.0
20	0.175	0.0	0.0	0.0	0.0
21	0.175	0.0	0.0	0.0	0.0
22	0.425	0.0	0.0	0.0	0.0
23	0.475	0.0	0.0	0.0	0.0
24	0.5	0.0	0.0	0.0	0.0
25	0.525	0.0	0.0	0.0	0.0
26	0.525	0.0	0.0	0.0	0.0
27	0.525	0.0	0.0	0.0	0.0
28	0.55	0.0	0.0	0.0	0.0
29	0.575	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTGGAG	60	0.0	35.000004	2
TGGTGGA	60	0.0	35.000004	1
GTGGAGC	60	0.0	35.000004	3
GCGGGAG	65	1.8189894E-12	32.307693	12
GTTGAAC	65	1.8189894E-12	32.307693	18
GAAATTC	65	1.8189894E-12	32.307693	33
AAATTCC	65	1.8189894E-12	32.307693	34
AATTCCT	65	1.8189894E-12	32.307693	35
GTCCGAA	65	1.8189894E-12	32.307693	29
GCTGGCG	65	1.8189894E-12	32.307693	8
GAGCTGG	65	1.8189894E-12	32.307693	6
AACCCGC	65	1.8189894E-12	32.307693	22
ACCCGCG	65	1.8189894E-12	32.307693	23
TGGAGCT	65	1.8189894E-12	32.307693	4
GGGAGTT	65	1.8189894E-12	32.307693	14
GAGTTGA	65	1.8189894E-12	32.307693	16
CTGGCGG	65	1.8189894E-12	32.307693	9
CCCGCGT	65	1.8189894E-12	32.307693	24
GAACCCG	65	1.8189894E-12	32.307693	21
CGGGAGT	65	1.8189894E-12	32.307693	13
>>END_MODULE
SRR23332709 read2 length is 41-50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332709_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41-50
%GC	54
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	19.815	11.0	11.0	37.0	11.0	37.0
2	22.264	25.0	11.0	37.0	11.0	37.0
3	25.078	25.0	11.0	37.0	11.0	37.0
4	35.4265	37.0	37.0	37.0	25.0	37.0
5	36.003	37.0	37.0	37.0	37.0	37.0
6	35.8695	37.0	37.0	37.0	37.0	37.0
7	35.4595	37.0	37.0	37.0	37.0	37.0
8	35.7315	37.0	37.0	37.0	37.0	37.0
9	35.3215	37.0	37.0	37.0	37.0	37.0
10	35.7375	37.0	37.0	37.0	37.0	37.0
11	35.6455	37.0	37.0	37.0	37.0	37.0
12	35.681	37.0	37.0	37.0	37.0	37.0
13	35.731	37.0	37.0	37.0	37.0	37.0
14	35.775	37.0	37.0	37.0	37.0	37.0
15	35.449	37.0	37.0	37.0	37.0	37.0
16	35.9005	37.0	37.0	37.0	37.0	37.0
17	35.757	37.0	37.0	37.0	37.0	37.0
18	35.92	37.0	37.0	37.0	37.0	37.0
19	35.9275	37.0	37.0	37.0	37.0	37.0
20	35.935	37.0	37.0	37.0	37.0	37.0
21	35.8415	37.0	37.0	37.0	37.0	37.0
22	35.9605	37.0	37.0	37.0	37.0	37.0
23	36.0205	37.0	37.0	37.0	37.0	37.0
24	35.982	37.0	37.0	37.0	37.0	37.0
25	35.7685	37.0	37.0	37.0	37.0	37.0
26	35.901	37.0	37.0	37.0	37.0	37.0
27	35.79725	37.0	37.0	37.0	37.0	37.0
28	35.9665	37.0	37.0	37.0	37.0	37.0
29	35.9785	37.0	37.0	37.0	37.0	37.0
30	35.8815	37.0	37.0	37.0	37.0	37.0
31	35.741	37.0	37.0	37.0	37.0	37.0
32	35.8005	37.0	37.0	37.0	37.0	37.0
33	35.58275	37.0	37.0	37.0	37.0	37.0
34	35.86375	37.0	37.0	37.0	37.0	37.0
35	36.087	37.0	37.0	37.0	37.0	37.0
36	36.031	37.0	37.0	37.0	37.0	37.0
37	35.966	37.0	37.0	37.0	37.0	37.0
38	35.7815	37.0	37.0	37.0	37.0	37.0
39	35.7775	37.0	37.0	37.0	37.0	37.0
40	35.8115	37.0	37.0	37.0	37.0	37.0
41	35.5245	37.0	37.0	37.0	37.0	37.0
42	35.79139569784893	37.0	37.0	37.0	37.0	37.0
43	35.51988991743808	37.0	37.0	37.0	37.0	37.0
44	35.877272727272725	37.0	37.0	37.0	37.0	37.0
45	35.53173152259075	37.0	37.0	37.0	37.0	37.0
46	35.68664563617245	37.0	37.0	37.0	37.0	37.0
47	35.81900212314225	37.0	37.0	37.0	37.0	37.0
48	35.858970941082376	37.0	37.0	37.0	37.0	37.0
49	35.75446685878963	37.0	37.0	37.0	37.0	37.0
50	35.52158401712451	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	0.0
18	3.0
19	3.0
20	8.0
21	7.0
22	10.0
23	9.0
24	14.0
25	16.0
26	22.0
27	29.0
28	41.0
29	42.0
30	47.0
31	66.0
32	95.0
33	158.0
34	459.0
35	1747.0
36	1091.0
37	131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	39.25	22.475	38.275
2	26.924999999999997	33.525	15.024999999999999	24.525
3	29.825000000000003	42.525	8.924999999999999	18.725
4	86.7	5.375	3.35	4.575
5	76.6	12.1	7.324999999999999	3.975
6	65.875	19.1	5.675	9.35
7	43.9	39.425	6.4750000000000005	10.2
8	42.925000000000004	20.25	10.85	25.974999999999998
9	19.675	39.875	29.15	11.3
10	45.475	22.475	15.35	16.7
11	26.700000000000003	44.2	15.675	13.425
12	22.375	23.875	29.9	23.849999999999998
13	39.4	10.549999999999999	31.424999999999997	18.625
14	34.975	12.0	25.825	27.200000000000003
15	29.125	13.05	49.325	8.5
16	51.4	18.375	21.4	8.825
17	29.65	34.375	15.775	20.200000000000003
18	13.575000000000001	44.800000000000004	27.750000000000004	13.875000000000002
19	16.8	14.575	41.4	27.224999999999998
20	12.375	20.599999999999998	36.475	30.55
21	18.5	41.55	9.6	30.349999999999998
22	44.875	29.825000000000003	11.600000000000001	13.700000000000001
23	20.25	34.599999999999994	18.0	27.150000000000002
24	23.325000000000003	20.674999999999997	9.275	46.725
25	45.775	13.125	9.049999999999999	32.05
26	39.0	19.175	10.575	31.25
27	46.21155288822206	23.830957739434858	18.02950737684421	11.927981995498875
28	34.849999999999994	27.500000000000004	26.700000000000003	10.95
29	22.55	11.1	53.900000000000006	12.45
30	18.375	12.75	58.4	10.475
31	16.425	19.950000000000003	45.550000000000004	18.075
32	37.574999999999996	10.925	18.025	33.475
33	21.030257564391096	10.577644411102776	11.952988247061766	56.439109777444365
34	28.546409807355516	15.761821366024517	19.714786089567177	35.976982737052786
35	9.75	37.95	20.724999999999998	31.574999999999996
36	11.3	46.650000000000006	26.450000000000003	15.6
37	13.275	51.824999999999996	9.45	25.45
38	21.75	15.325	8.0	54.925000000000004
39	36.1	13.05	8.725	42.125
40	21.2	20.05	15.65	43.1
41	14.875	46.075	26.674999999999997	12.375
42	20.335167583791897	31.59079539769885	28.53926963481741	19.534767383691847
43	37.42807105328997	21.26594946209657	13.084813610207654	28.221165874405806
44	42.803030303030305	11.287878787878789	17.929292929292927	27.979797979797983
45	16.21833376860799	12.144162966832072	39.409767563332466	32.22773570122747
46	33.412197686645634	15.878023133543639	31.04626708727655	19.663512092534173
47	29.830148619957537	12.765392781316349	24.469214437367302	32.93524416135881
48	26.872833910957077	28.23247134097574	25.673153825646494	19.221540922420687
49	30.144092219020173	8.472622478386167	46.59942363112392	14.783861671469742
50	36.24687834463075	11.166607206564395	27.434891188012845	25.15162326079201
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.5
27	2.0
28	3.0
29	4.5
30	6.0
31	7.0
32	8.5
33	12.5
34	17.0
35	21.0
36	24.5
37	27.5
38	31.0
39	35.0
40	42.5
41	54.5
42	64.5
43	74.0
44	79.5
45	77.5
46	77.5
47	104.0
48	133.0
49	151.5
50	178.0
51	362.5
52	567.0
53	762.5
54	959.0
55	965.0
56	916.5
57	763.5
58	580.0
59	388.0
60	213.5
61	127.0
62	70.0
63	47.5
64	29.5
65	23.5
66	17.5
67	11.5
68	5.5
69	4.5
70	5.5
71	4.5
72	3.0
73	2.5
74	1.5
75	0.5
76	0.0
77	0.5
78	1.0
79	1.5
80	2.0
81	1.0
82	0.0
83	1.0
84	2.0
85	1.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	2.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.025
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.025
34	0.075
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
41	2.0
42	1.0
43	37.0
44	131.0
45	25.0
46	36.0
47	17.0
48	281.0
49	667.0
50	2803.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.275000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.14104515474378	40.475
2	6.1897513952308465	6.1
3	2.5875190258751903	3.8249999999999997
4	1.8772196854388634	3.6999999999999997
5	1.0147133434804667	2.5
6	1.06544901065449	3.15
7	0.9639776763064434	3.325
8	0.6595636732623034	2.6
9	0.4058853373921867	1.7999999999999998
>10	2.9426686960933535	25.974999999999998
>50	0.10147133434804667	3.325
>100	0.050735667174023336	3.225
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	129	3.225	No Hit
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	74	1.8499999999999999	No Hit
AAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	59	1.4749999999999999	No Hit
CAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	48	1.2	No Hit
CCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	47	1.175	No Hit
CCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	45	1.125	No Hit
ACAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	36	0.8999999999999999	No Hit
AAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	35	0.8750000000000001	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	33	0.8250000000000001	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	30	0.75	No Hit
ATAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	30	0.75	No Hit
AACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	28	0.7000000000000001	No Hit
CCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	27	0.675	No Hit
CACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	25	0.625	No Hit
TAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	22	0.5499999999999999	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	20	0.5	No Hit
CAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	20	0.5	No Hit
TAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	20	0.5	No Hit
CGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	19	0.475	No Hit
CGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	19	0.475	No Hit
CTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	18	0.44999999999999996	No Hit
CCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	18	0.44999999999999996	No Hit
CAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	18	0.44999999999999996	No Hit
CGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	17	0.42500000000000004	No Hit
CTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	17	0.42500000000000004	No Hit
CCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	16	0.4	No Hit
ACCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
CGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
CCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	16	0.4	No Hit
TTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
AAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
CGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
CGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
TCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
CGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	14	0.35000000000000003	No Hit
AGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
ACAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	14	0.35000000000000003	No Hit
CAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
CCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	13	0.325	No Hit
CAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	13	0.325	No Hit
AATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
CTCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
CCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	13	0.325	No Hit
ACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
AATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
CGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
TGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
CCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
ACAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	11	0.27499999999999997	No Hit
TGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
AAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
ATAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
ATAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
TGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
CAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
TCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
TAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
CGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
CGCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
TCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
AACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
TGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CCCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
TAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CCAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	9	0.22499999999999998	No Hit
CACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
ACAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	9	0.22499999999999998	No Hit
ATTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
TCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
TAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
AGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
TGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
AGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
TAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CACGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
CCCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
CACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CCGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
AAAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAT	7	0.17500000000000002	No Hit
CCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
AAAGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCT	7	0.17500000000000002	No Hit
CGCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
AAAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGT	7	0.17500000000000002	No Hit
AGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
CTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
CATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
TAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
ACCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
ACAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	7	0.17500000000000002	No Hit
CGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
TTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
AAAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	6	0.15	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTA	6	0.15	No Hit
CTAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CTAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
CGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
ACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
ATCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TGCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
ACCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CGAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
AGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TTTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AACGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
CTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TTCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TCCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTA	5	0.125	No Hit
CGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TGAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TAAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	5	0.125	No Hit
AGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.075	0.0	0.0	0.0	0.0
11	0.075	0.0	0.0	0.0	0.0
12	0.1	0.0	0.0	0.0	0.0
13	0.15	0.0	0.0	0.0	0.0
14	0.15	0.0	0.0	0.0	0.0
15	0.15	0.0	0.0	0.0	0.0
16	0.15	0.0	0.0	0.0	0.0
17	0.15	0.0	0.0	0.0	0.0
18	0.15	0.0	0.0	0.0	0.0
19	0.15	0.0	0.0	0.0	0.0
20	0.15	0.0	0.0	0.0	0.0
21	0.15	0.0	0.0	0.0	0.0
22	0.15	0.0	0.0	0.0	0.0
23	0.15	0.0	0.0	0.0	0.0
24	0.15	0.0	0.0	0.0	0.0
25	0.15	0.0	0.0	0.0	0.0
26	0.15	0.0	0.0	0.0	0.0
27	0.175	0.0	0.0	0.0	0.0
28	0.2	0.0	0.0	0.0	0.0
29	0.225	0.0	0.0	0.0	0.0
30	0.225	0.0	0.0	0.0	0.0
31	0.425	0.0	0.0	0.0	0.0
32	0.45	0.0	0.0	0.0	0.0
33	0.475	0.0	0.0	0.0	0.0
34	0.475	0.0	0.0	0.0	0.0
35	0.5	0.0	0.0	0.0	0.0
36	0.5	0.0	0.0	0.0	0.0
37	0.5	0.0	0.0	0.0	0.0
38	0.575	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCATG	85	0.0	51.27941	44
CGTAGTG	35	5.1721145E-8	47.767124	43
GTAGTGC	40	4.9652954E-8	47.673832	44
ACCGTAG	35	8.038842E-8	45.285717	41
CCGTAGT	35	8.038842E-8	45.285717	42
CACCGTA	35	8.941788E-8	44.705128	40
AGGGGCT	25	3.90739E-5	43.5875	3
CGGGGCT	50	1.4551915E-11	43.5875	3
CCCGGGG	20	7.2824064E-4	43.5875	1
AAGGGGC	20	7.2824064E-4	43.5875	2
AAGGGAG	75	0.0	43.5875	2
AAAGGGA	60	0.0	43.5875	1
ACGGGGC	20	7.2824064E-4	43.5875	2
CCGGGGC	25	3.90739E-5	43.5875	2
CACGGGG	25	3.90739E-5	43.5875	1
AGGGAGA	105	0.0	43.587498	3
GCTGCGC	45	1.255903E-7	42.376736	44
GGTGCAT	95	0.0	40.22495	43
GCACCGT	40	2.5562622E-7	39.11699	39
TCTGGAT	90	0.0	38.744446	13
>>END_MODULE
Read 622433 spots for SRR23332709.sra
Written 622433 spots for SRR23332709.sra
Read 622433 spots for SRR23332709.sra
Written 622433 spots for SRR23332709.sra
Read 622433 spots for SRR23332709.sra
Written 622433 spots for SRR23332709.sra
Read 622433 spots for SRR23332709.sra
Written 622433 spots for SRR23332709.sra
Read 622433 spots for SRR23332709.sra
Written 622433 spots for SRR23332709.sra
Read 622433 spots for SRR23332709.sra
Written 622433 spots for SRR23332709.sra
Read 622439 spots for SRR23332709.sra
Written 622439 spots for SRR23332709.sra
Read 622433 spots for SRR23332709.sra
Written 622433 spots for SRR23332709.sra
SRR ids: ['SRR23332709.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_jfl6vbwl
SRR23332709.sra spots: 4979470
blocks: [[1, 622433], [622434, 1244866], [1244867, 1867299], [1867300, 2489732], [2489733, 3112165], [3112166, 3734598], [3734599, 4357031], [4357032, 4979470]]
SRR23332709 file size 605676
SRR23332709 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR23332709 SRR23332709_1.fastq SRR23332709_2.fastq
Input file:	SRR23332709_1.fastq
Paired file:	SRR23332709_2.fastq
trimmed:	SRR23332709-trimmed-pair1.fastq, SRR23332709-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 03:47:37 2026 >> started

Wed Mar 25 03:47:39 2026 >> done (2.665s)
4979470 read pairs processed; of these:
    332 ( 0.01%) short read pairs filtered out after trimming by size control
      7 ( 0.00%) empty read pairs filtered out after trimming by size control
4979131 (99.99%) read pairs available; of these:
  44633 ( 0.90%) trimmed read pairs available after processing
4934498 (99.10%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     91	  0.00%
 19	    245	  0.00%
 20	    630	  0.01%
 21	  11832	  0.24%
 22	    307	  0.01%
 23	    380	  0.01%
 24	    432	  0.01%
 25	    578	  0.01%
 26	    465	  0.01%
 27	    634	  0.01%
 28	    536	  0.01%
 29	   2577	  0.05%
 30	    839	  0.02%
 31	    972	  0.02%
 32	    987	  0.02%
 33	   1177	  0.02%
 34	   1939	  0.04%
 35	   1607	  0.03%
 36	   1589	  0.03%
 37	   1624	  0.03%
 38	   1987	  0.04%
 39	   2270	  0.05%
 40	   7215	  0.14%
 41	   5856	  0.12%
 42	 238538	  4.79%
 43	  79557	  1.60%
 44	 462739	  9.29%
 45	4151528	 83.38%
4979131 reads passed initial QC


criterion=sequence-density
sequence-density=1.66
sequence-density-rank=1
fanout-score=2.51
fanout-score-rank=16
prefix-density=1.45
prefix-fanout=2.5
sequence=ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=37
fanout-score=30.45
fanout-score-rank=1
prefix-density=0.28
prefix-fanout=1.1
sequence=TTTTTTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACAGAATCAGGCCATTACTACCCTGTCCGCCATGGCTTCTCGCCAGCCTCCCAGCCATTGTGACCTTTGATTCAGCGTCTGATAGGGACACATTTCTTTTGAGCGTCCGGCGATGCCGGCCTGATAACCACGTTGATGTGCCCGTTCCAGGCGATCTCGTTTTTGTCTCTTCATGCCTCGTTTC


criterion=sequence-density
sequence-density=23.32
sequence-density-rank=1
fanout-score=20.22
fanout-score-rank=7
prefix-density=19.14
prefix-fanout=20.2
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC


criterion=fanout-score
sequence-density=0.67
sequence-density-rank=12
fanout-score=202.98
fanout-score-rank=1
prefix-density=11.70
prefix-fanout=11.6
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTAACTCCTGGA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG -y GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC -o SRR23332709 SRR23332709_1.fastq SRR23332709_2.fastq
Input file:	SRR23332709_1.fastq
Paired file:	SRR23332709_2.fastq
trimmed:	SRR23332709-trimmed-pair1.fastq, SRR23332709-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG
-- paired 3' end adapter sequence (-y):	GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 03:47:48 2026 >> started

Wed Mar 25 03:47:51 2026 >> done (2.557s)
4213111 read pairs processed; of these:
  66699 ( 1.58%) short read pairs filtered out after trimming by size control
   3999 ( 0.09%) empty read pairs filtered out after trimming by size control
4142413 (98.32%) read pairs available; of these:
   1142 ( 0.03%) trimmed read pairs available after processing
4141271 (99.97%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     78	  0.00%
 19	    213	  0.01%
 20	    551	  0.01%
 21	  10015	  0.24%
 22	    270	  0.01%
 23	    328	  0.01%
 24	    361	  0.01%
 25	    485	  0.01%
 26	    385	  0.01%
 27	    537	  0.01%
 28	    449	  0.01%
 29	   2176	  0.05%
 30	    724	  0.02%
 31	    826	  0.02%
 32	    843	  0.02%
 33	   1001	  0.02%
 34	   1672	  0.04%
 35	   1371	  0.03%
 36	   1363	  0.03%
 37	   1382	  0.03%
 38	   1659	  0.04%
 39	   1920	  0.05%
 40	   6071	  0.15%
 41	   4923	  0.12%
 42	 200207	  4.83%
 43	  67281	  1.62%
 44	 372042	  8.98%
 45	3463280	 83.61%


criterion=sequence-density
sequence-density=1.61
sequence-density-rank=1
fanout-score=1.91
fanout-score-rank=30
prefix-density=1.30
prefix-fanout=1.9
sequence=GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=35
fanout-score=27.64
fanout-score-rank=1
prefix-density=0.26
prefix-fanout=1.1
sequence=TTTTTTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACAGAATCAGGCCATTACTACCCTGTCCGCCATGGCTTCTCGCCAGCCTCCCAGCCATTGTGACCTTTGATTCAGCGTCTGATAGGGACACATTTCTTTTGAGCGTCCGGCGATGCCGGCCTGATAACCACGTTGATGTGCCCGTTCCAGGCGATCTCGTTTTTGTCTCTTCATGCCTCGTTTCC


criterion=sequence-density
sequence-density=22.20
sequence-density-rank=1
fanout-score=19.35
fanout-score-rank=7
prefix-density=18.78
prefix-fanout=19.4
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT


criterion=fanout-score
sequence-density=0.67
sequence-density-rank=12
fanout-score=202.72
fanout-score-rank=1
prefix-density=11.83
prefix-fanout=11.6
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTAACTCCTGGA
SRR23332709 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 25 03:48:07
                             Started mapping on |	Mar 25 03:48:07
                                    Finished on |	Mar 25 03:50:49
       Mapping speed, Million of reads per hour |	109.08

                          Number of input reads |	4908433
                      Average input read length |	90
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2490535
                        Uniquely mapped reads % |	50.74%
                          Average mapped length |	87.64
                       Number of splices: Total |	187028
            Number of splices: Annotated (sjdb) |	208
                       Number of splices: GT/AG |	173725
                       Number of splices: GC/AG |	2995
                       Number of splices: AT/AC |	676
               Number of splices: Non-canonical |	9632
                      Mismatch rate per base, % |	0.32%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.22
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1130080
             % of reads mapped to multiple loci |	23.02%
        Number of reads mapped to too many loci |	968685
             % of reads mapped to too many loci |	19.74%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.19%
                     % of reads unmapped: other |	0.31%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1287818	1287818	1287818
N_multimapping	1130080	1130080	1130080
N_noFeature	61803	2243019	160756
N_ambiguous	228371	44024	47871
UnstrandedReadsAssigned:2200361 PositiveStrandReadsAssigned:203492 NegativeStrandReadsAssigned:2281908
Dataset is classified negative stranded
MeadianReadLen=41 20thPercentileLength=41 echo kmer=37
SRR23332709 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR23332709-trimmed-pair1.fastq
                             SRR23332709-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,908,433 reads, 435,198 reads pseudoaligned
[quant] estimated average fragment length: 223.238
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 127 rounds

  4323 SRR23332709.ke.tsv
  4497 SRR23332709.se.tsv
  8820 total
==> SRR23332709.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	12.2059	104	2731.27
AAC73113	2463	2240.76	64	9.15556
AAC73114	933	710.812	50	22.5484
AAC73115	1287	1064.76	66	19.8697
AAC73116	297	112.578	6	17.0844
AAC73117	777	555.092	21	12.127
AAC73118	1431	1208.76	10	2.65192
AAC73119	954	731.788	420	183.978
AAC73120	588	368.541	39	33.9218

==> SRR23332709.se.tsv <==
b0001	153
b0002	53
b0003	42
b0004	63
b0005	8
b0006	29
b0007	12
b0008	416
b0009	61
b0010	16
SRR23332709 completed mapping pipeline successfully
