Starting /dee2/code/volunteer_pipeline.sh SRR23332737
    current disk space = 1464389004
    free memory = 58350108 
SRR23332737 SRAfilesize
d2a8056009aa4cc1ca218dd5308f5a49  SRR23332737.sra
SRR23332737.sra file validated
SRR23332737 is paired end
SRR23332737 is conventional basespace
SRR23332737 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332737_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.345	37.0	37.0	37.0	37.0	37.0
2	36.3965	37.0	37.0	37.0	37.0	37.0
3	36.488	37.0	37.0	37.0	37.0	37.0
4	36.45	37.0	37.0	37.0	37.0	37.0
5	36.3575	37.0	37.0	37.0	37.0	37.0
6	36.257	37.0	37.0	37.0	37.0	37.0
7	36.3285	37.0	37.0	37.0	37.0	37.0
8	36.3655	37.0	37.0	37.0	37.0	37.0
9	36.467	37.0	37.0	37.0	37.0	37.0
10	36.465	37.0	37.0	37.0	37.0	37.0
11	36.3955	37.0	37.0	37.0	37.0	37.0
12	36.3965	37.0	37.0	37.0	37.0	37.0
13	36.461	37.0	37.0	37.0	37.0	37.0
14	36.3225	37.0	37.0	37.0	37.0	37.0
15	36.514	37.0	37.0	37.0	37.0	37.0
16	36.46	37.0	37.0	37.0	37.0	37.0
17	36.366	37.0	37.0	37.0	37.0	37.0
18	36.3455	37.0	37.0	37.0	37.0	37.0
19	36.235	37.0	37.0	37.0	37.0	37.0
20	36.3125	37.0	37.0	37.0	37.0	37.0
21	36.406	37.0	37.0	37.0	37.0	37.0
22	36.3505	37.0	37.0	37.0	37.0	37.0
23	36.316	37.0	37.0	37.0	37.0	37.0
24	36.285	37.0	37.0	37.0	37.0	37.0
25	36.3375	37.0	37.0	37.0	37.0	37.0
26	36.2655	37.0	37.0	37.0	37.0	37.0
27	36.371	37.0	37.0	37.0	37.0	37.0
28	36.432	37.0	37.0	37.0	37.0	37.0
29	36.3385	37.0	37.0	37.0	37.0	37.0
30	36.3615	37.0	37.0	37.0	37.0	37.0
31	36.3645	37.0	37.0	37.0	37.0	37.0
32	36.2915	37.0	37.0	37.0	37.0	37.0
33	36.316	37.0	37.0	37.0	37.0	37.0
34	36.2635	37.0	37.0	37.0	37.0	37.0
35	36.35	37.0	37.0	37.0	37.0	37.0
36	36.2505	37.0	37.0	37.0	37.0	37.0
37	36.322	37.0	37.0	37.0	37.0	37.0
38	36.287	37.0	37.0	37.0	37.0	37.0
39	36.225	37.0	37.0	37.0	37.0	37.0
40	36.2245	37.0	37.0	37.0	37.0	37.0
41	36.2265	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	1.0
23	0.0
24	2.0
25	5.0
26	2.0
27	9.0
28	15.0
29	25.0
30	24.0
31	32.0
32	45.0
33	89.0
34	113.0
35	234.0
36	1330.0
37	2073.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.3	19.825	45.800000000000004	10.075000000000001
2	28.075	18.75	29.175	24.0
3	29.625	20.674999999999997	28.249999999999996	21.45
4	21.75	21.6	34.475	22.175
5	32.75	22.5	22.650000000000002	22.1
6	31.75	22.1	22.3	23.849999999999998
7	23.775	31.45	20.549999999999997	24.224999999999998
8	35.0	19.425	19.8	25.775
9	26.700000000000003	16.125	20.775	36.4
10	26.5	16.875	32.15	24.474999999999998
11	37.45	18.325	20.075000000000003	24.15
12	35.65	19.45	21.224999999999998	23.674999999999997
13	24.5	20.525	22.775000000000002	32.2
14	36.725	21.224999999999998	20.75	21.3
15	35.425000000000004	22.25	21.9	20.424999999999997
16	36.475	23.0	21.45	19.075
17	25.25	31.724999999999998	22.725	20.3
18	34.975	21.575	22.975	20.474999999999998
19	25.174999999999997	20.674999999999997	31.874999999999996	22.275
20	25.825	20.4	31.15	22.625
21	35.4	21.125	20.7	22.775000000000002
22	23.799999999999997	31.525	21.55	23.125
23	24.825	30.125	21.4	23.65
24	25.3	20.7	19.05	34.949999999999996
25	28.175	21.15	18.575	32.1
26	25.3	21.099999999999998	21.175	32.425
27	36.05	21.099999999999998	21.0	21.85
28	24.8	19.675	20.674999999999997	34.849999999999994
29	35.475	19.05	22.25	23.225
30	24.5	21.099999999999998	32.375	22.025
31	24.125	21.05	22.55	32.275
32	24.775	24.725	19.625	30.875000000000004
33	33.15	23.95	20.7	22.2
34	22.1	33.15	22.2	22.55
35	23.575	31.175000000000004	23.0	22.25
36	23.275000000000002	31.374999999999996	20.775	24.575
37	23.5	22.25	31.5	22.75
38	24.025	21.575	30.625000000000004	23.775
39	22.45	21.9	22.1	33.550000000000004
40	22.225	22.225	21.4	34.150000000000006
41	23.849999999999998	20.674999999999997	31.275	24.2
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	2.0
24	3.0
25	3.0
26	4.0
27	5.0
28	5.5
29	6.0
30	14.5
31	23.0
32	23.0
33	27.5
34	32.0
35	44.0
36	56.0
37	56.0
38	73.0
39	90.0
40	118.0
41	146.0
42	146.0
43	194.5
44	243.0
45	284.5
46	326.0
47	326.0
48	366.0
49	406.0
50	428.5
51	451.0
52	470.0
53	489.0
54	489.0
55	451.5
56	414.0
57	381.0
58	348.0
59	348.0
60	528.5
61	709.0
62	421.0
63	133.0
64	133.0
65	102.5
66	72.0
67	50.5
68	29.0
69	29.0
70	19.5
71	10.0
72	8.5
73	7.0
74	3.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.5
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.02163371876165	54.974999999999994
2	12.27154046997389	16.45
3	2.909362178291682	5.8500000000000005
4	1.0443864229765014	2.8000000000000003
5	0.5594927265945543	1.875
6	0.29839612085042894	1.2
7	0.3356956359567326	1.575
8	0.07459903021260723	0.4
9	0.11189854531891084	0.675
>10	0.3356956359567326	3.8249999999999997
>50	0.0	0.0
>100	0.03729951510630362	10.375
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCT	415	10.375	No Hit
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTAC	33	0.8250000000000001	No Hit
GGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTA	31	0.775	No Hit
AGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGT	16	0.4	No Hit
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCT	16	0.4	TruSeq Adapter, Index 8 (97% over 37bp)
AAGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCC	15	0.375	No Hit
AGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAA	11	0.27499999999999997	No Hit
GGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACAT	11	0.27499999999999997	No Hit
GGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAAC	10	0.25	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCC	10	0.25	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACGAGAGGACCGGGA	9	0.22499999999999998	No Hit
TTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCAC	9	0.22499999999999998	No Hit
AAGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGA	9	0.22499999999999998	No Hit
TTTATCACTGGAGGAAGGTGGGGATGACGTCAAGTCATCAT	8	0.2	No Hit
TCATGGAGTCGAGTTGCTAGTAATCGTGGATCAGAATGCCA	8	0.2	No Hit
GCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACTAC	7	0.17500000000000002	No Hit
TCTGATCCACGGTGAATACGTTCCCGGGCCTTGTACACACC	7	0.17500000000000002	No Hit
TACTTCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTG	7	0.17500000000000002	No Hit
TGTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCT	7	0.17500000000000002	No Hit
AAAAAACGCGCCCGAAGGCGCGTTGGCGATACACTCAATAT	7	0.17500000000000002	No Hit
GTGTGTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCA	7	0.17500000000000002	No Hit
GTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTC	7	0.17500000000000002	No Hit
TGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACA	7	0.17500000000000002	No Hit
ATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA	7	0.17500000000000002	No Hit
GACAGGAACCCTTGGTGTTACTGCGAAGGGGGGACGGAGAA	6	0.15	No Hit
TCCGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTA	6	0.15	No Hit
AAAAAATGGCGCACAATGTGCGCCATTTTTCACTTCACAGG	6	0.15	No Hit
CCTACTTCTTTTGCAACCCACTTTGTGATTCATGACTGGGG	6	0.15	No Hit
ATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCA	6	0.15	No Hit
TCATGGAGTCGAGTTGCAAAAGAAGTAGGTAGCTTAACCTT	6	0.15	No Hit
AAAACTTCGCAGTGAACCTTTGCAGGTACACTCTGAAGTAT	6	0.15	No Hit
TTCTGATCCACGGTGAATACGTTCCCGGGCCTTGTACACAC	6	0.15	No Hit
CTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGTC	5	0.125	No Hit
GTGTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACC	5	0.125	No Hit
TTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGTCG	5	0.125	No Hit
GCCACTAACAAACTAGCCTGATTAAGTTTTAACGCTTCAAC	5	0.125	No Hit
GAGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGG	5	0.125	No Hit
GTATTCACCGTGGGAGTGGGTTGCAAAAGAAGTAGGTAGCT	5	0.125	No Hit
TACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACTGG	5	0.125	No Hit
GCAGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCA	5	0.125	No Hit
CAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGC	5	0.125	No Hit
TCTCGTACTAGGAGTGGACGCATCACTGGTGTTCGGGTTGT	5	0.125	No Hit
TCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGACT	5	0.125	No Hit
TATCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGA	5	0.125	No Hit
GATCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCC	5	0.125	No Hit
ACCGGGCAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCC	5	0.125	No Hit
GGCGGTGTGTACAAGGTAACCGTAGGGGAACCTGCGGTTGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.425	0.0	0.0	0.0	0.0
5	0.425	0.0	0.0	0.0	0.0
6	0.425	0.0	0.0	0.0	0.0
7	0.425	0.0	0.0	0.0	0.0
8	0.45	0.0	0.0	0.0	0.0
9	0.45	0.0	0.0	0.0	0.0
10	0.45	0.0	0.0	0.0	0.0
11	0.45	0.0	0.0	0.0	0.0
12	0.45	0.0	0.0	0.0	0.0
13	0.475	0.0	0.0	0.0	0.0
14	0.5	0.0	0.0	0.0	0.0
15	0.6	0.0	0.0	0.0	0.0
16	0.625	0.0	0.0	0.0	0.0
17	0.675	0.0	0.0	0.0	0.0
18	0.75	0.0	0.0	0.0	0.0
19	0.8	0.0	0.0	0.0	0.0
20	0.875	0.0	0.0	0.0	0.0
21	0.975	0.0	0.0	0.0	0.0
22	1.075	0.0	0.0	0.0	0.0
23	1.15	0.0	0.0	0.0	0.0
24	1.3	0.0	0.0	0.0	0.0
25	1.475	0.0	0.0	0.0	0.0
26	1.525	0.0	0.0	0.0	0.0
27	1.55	0.0	0.0	0.0	0.0
28	1.625	0.0	0.0	0.0	0.0
29	1.725	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTGGA	25	1.4254966E-4	35.0	1
GGTGGAG	30	4.1612564E-4	29.166668	2
GTGGAGC	35	0.0010258359	25.000002	3
GAGTTGA	45	1.5026014E-4	23.333334	16
AGTTGAA	45	1.5026014E-4	23.333334	17
GCGGGAG	40	0.0022346217	21.875	12
GTTGAAC	40	0.0022346217	21.875	18
GTCCGAA	40	0.0022346217	21.875	29
GCTGGCG	40	0.0022346217	21.875	8
AACCCGC	40	0.0022346217	21.875	22
ACCCGCG	40	0.0022346217	21.875	23
TGGAGCT	40	0.0022346217	21.875	4
GGGAGTT	40	0.0022346217	21.875	14
CTGGCGG	40	0.0022346217	21.875	9
CCCGCGT	40	0.0022346217	21.875	24
GAACCCG	40	0.0022346217	21.875	21
CGGGAGT	40	0.0022346217	21.875	13
TTGAACC	40	0.0022346217	21.875	19
TGGCGGG	40	0.0022346217	21.875	10
TGAACCC	40	0.0022346217	21.875	20
>>END_MODULE
SRR23332737 read2 length is 42-50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332737_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	42-50
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.045	11.0	11.0	37.0	11.0	37.0
2	23.17	25.0	11.0	37.0	11.0	37.0
3	25.7455	25.0	11.0	37.0	11.0	37.0
4	35.604	37.0	37.0	37.0	37.0	37.0
5	35.9895	37.0	37.0	37.0	37.0	37.0
6	35.906	37.0	37.0	37.0	37.0	37.0
7	35.8635	37.0	37.0	37.0	37.0	37.0
8	35.838	37.0	37.0	37.0	37.0	37.0
9	35.752	37.0	37.0	37.0	37.0	37.0
10	35.9295	37.0	37.0	37.0	37.0	37.0
11	35.8855	37.0	37.0	37.0	37.0	37.0
12	35.944	37.0	37.0	37.0	37.0	37.0
13	36.0565	37.0	37.0	37.0	37.0	37.0
14	35.999	37.0	37.0	37.0	37.0	37.0
15	35.818	37.0	37.0	37.0	37.0	37.0
16	36.067	37.0	37.0	37.0	37.0	37.0
17	36.021	37.0	37.0	37.0	37.0	37.0
18	36.103	37.0	37.0	37.0	37.0	37.0
19	35.959	37.0	37.0	37.0	37.0	37.0
20	35.99825	37.0	37.0	37.0	37.0	37.0
21	35.89075	37.0	37.0	37.0	37.0	37.0
22	36.02	37.0	37.0	37.0	37.0	37.0
23	35.978	37.0	37.0	37.0	37.0	37.0
24	36.13	37.0	37.0	37.0	37.0	37.0
25	35.9345	37.0	37.0	37.0	37.0	37.0
26	36.04975	37.0	37.0	37.0	37.0	37.0
27	36.101	37.0	37.0	37.0	37.0	37.0
28	36.07075	37.0	37.0	37.0	37.0	37.0
29	35.89475	37.0	37.0	37.0	37.0	37.0
30	36.04675	37.0	37.0	37.0	37.0	37.0
31	35.98375	37.0	37.0	37.0	37.0	37.0
32	35.817	37.0	37.0	37.0	37.0	37.0
33	35.7785	37.0	37.0	37.0	37.0	37.0
34	36.16675	37.0	37.0	37.0	37.0	37.0
35	36.05975	37.0	37.0	37.0	37.0	37.0
36	36.11125	37.0	37.0	37.0	37.0	37.0
37	36.016	37.0	37.0	37.0	37.0	37.0
38	35.9435	37.0	37.0	37.0	37.0	37.0
39	35.997	37.0	37.0	37.0	37.0	37.0
40	35.8975	37.0	37.0	37.0	37.0	37.0
41	35.851	37.0	37.0	37.0	37.0	37.0
42	35.917	37.0	37.0	37.0	37.0	37.0
43	35.77527527527528	37.0	37.0	37.0	37.0	37.0
44	35.84189325276939	37.0	37.0	37.0	37.0	37.0
45	35.7071129707113	37.0	37.0	37.0	37.0	37.0
46	35.813424947145876	37.0	37.0	37.0	37.0	37.0
47	35.978586723768736	37.0	37.0	37.0	37.0	37.0
48	35.926307233811976	37.0	37.0	37.0	37.0	37.0
49	35.88101644245142	37.0	37.0	37.0	37.0	37.0
50	35.814842300556585	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	3.0
20	6.0
21	2.0
22	13.0
23	6.0
24	14.0
25	11.0
26	6.0
27	17.0
28	31.0
29	32.0
30	47.0
31	63.0
32	82.0
33	156.0
34	442.0
35	1760.0
36	1127.0
37	180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	39.4	23.325000000000003	37.275000000000006
2	29.925	32.5	13.700000000000001	23.875
3	29.675	41.9	10.549999999999999	17.875
4	81.6	6.800000000000001	4.925	6.675000000000001
5	67.30000000000001	16.75	9.775	6.175
6	56.25	22.15	10.025	11.575000000000001
7	38.125	40.550000000000004	10.274999999999999	11.05
8	43.85	20.925	13.4	21.825
9	20.65	40.1	25.7	13.55
10	40.75	21.875	18.175	19.2
11	28.999999999999996	37.925	17.45	15.625
12	24.825	25.124999999999996	24.55	25.5
13	40.175	14.05	26.474999999999998	19.3
14	37.175000000000004	13.0	26.85	22.975
15	30.375000000000004	17.5	40.925	11.200000000000001
16	44.9	22.375	21.3	11.425
17	25.05	34.300000000000004	19.3	21.349999999999998
18	15.85	38.45	27.425	18.275
19	19.725	17.95	33.175	29.15
20	16.454113528382095	23.730932733183295	30.75768942235559	29.057264316079017
21	20.305076269067268	41.53538384596149	14.078519629907477	24.081020255063766
22	39.15	27.875	14.000000000000002	18.975
23	23.275000000000002	27.450000000000003	19.950000000000003	29.325000000000003
24	24.3	23.175	13.525	39.0
25	35.775	16.85	14.499999999999998	32.875
26	32.00800200050013	22.18054513628407	13.928482120530134	31.882970742685675
27	39.24462231115558	22.936468234117058	22.0360180090045	15.78289144572286
28	35.55888972243061	22.80570142535634	27.35683920980245	14.278569642410602
29	24.831207801950487	15.65391347836959	44.63615903975994	14.87871967991998
30	19.30482620655164	17.029257314328582	49.437359339834956	14.228557139284822
31	18.95473868467117	22.155538884721178	38.159539884971245	20.730182545636406
32	32.84142071035518	11.655827913956978	20.485242621310658	35.01750875437719
33	23.08654327163582	12.756378189094548	16.3831915957979	47.77388694347174
34	25.619214410808105	16.162121591193397	22.61696272204153	35.60170127595696
35	12.77819454863716	31.13278319579895	23.58089522380595	32.50812703175794
36	13.103275818954737	39.109777444361086	29.35733933483371	18.42960740185046
37	16.775000000000002	43.775	13.025	26.424999999999997
38	23.225	19.775000000000002	11.575000000000001	45.425
39	38.2	17.5	12.174999999999999	32.125
40	23.9	19.675	20.05	36.375
41	17.25	38.65	28.299999999999997	15.8
42	21.425	26.974999999999998	30.15	21.45
43	30.130130130130127	21.496496496496498	17.54254254254254	30.83083083083083
44	37.38670694864049	13.544813695871097	19.73816717019134	29.330312185297082
45	18.331589958158993	15.638075313807532	34.30962343096235	31.72071129707113
46	30.681818181818183	17.706131078224104	34.381606765327696	17.23044397463002
47	34.42184154175589	14.962526766595289	25.267665952890795	25.34796573875803
48	29.62059620596206	22.24932249322493	26.856368563685635	21.27371273712737
49	31.36961722488038	12.529904306220097	39.71291866028708	16.38755980861244
50	28.645640074211503	14.545454545454545	27.198515769944343	29.61038961038961
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	1.0
24	2.0
25	1.5
26	1.0
27	2.0
28	4.0
29	9.0
30	13.0
31	17.5
32	26.5
33	32.5
34	35.0
35	42.5
36	45.5
37	41.0
38	48.5
39	69.0
40	76.5
41	77.5
42	92.5
43	111.0
44	120.5
45	119.5
46	117.5
47	127.0
48	140.5
49	176.5
50	223.0
51	349.0
52	479.5
53	649.5
54	830.5
55	843.0
56	783.0
57	660.0
58	546.0
59	394.5
60	251.0
61	168.5
62	100.5
63	73.0
64	53.5
65	44.5
66	35.5
67	24.5
68	14.5
69	9.5
70	7.5
71	6.0
72	2.5
73	1.5
74	1.5
75	1.0
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	1.0
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.5
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	2.5
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.025
21	0.025
22	0.0
23	0.0
24	0.0
25	0.0
26	0.025
27	0.05
28	0.025
29	0.025
30	0.025
31	0.025
32	0.05
33	0.05
34	0.075
35	0.025
36	0.025
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0270929287455974
49	0.029895366218236175
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
42	4.0
43	24.0
44	148.0
45	40.0
46	48.0
47	45.0
48	346.0
49	650.0
50	2695.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.775000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.89963577498987	54.300000000000004
2	4.208822339133953	5.2
3	1.9425333872925938	3.5999999999999996
4	1.0117361392148927	2.5
5	0.84985835694051	2.625
6	0.7689194658033185	2.85
7	0.7284500202347227	3.15
8	0.56657223796034	2.8000000000000003
9	0.28328611898017	1.575
>10	1.6187778227438283	16.475
>50	0.12140833670578711	4.925
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	73	1.825	No Hit
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	72	1.7999999999999998	No Hit
AAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	52	1.3	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	45	1.125	No Hit
AAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	43	1.075	No Hit
CGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	28	0.7000000000000001	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	27	0.675	No Hit
CCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	26	0.65	No Hit
CCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	26	0.65	No Hit
ACAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	24	0.6	No Hit
ACAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	23	0.575	No Hit
CAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	20	0.5	No Hit
CCCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	19	0.475	No Hit
CCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	19	0.475	No Hit
CCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	16	0.4	No Hit
TAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
CGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	16	0.4	No Hit
CAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	16	0.4	No Hit
TCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
CGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	15	0.375	No Hit
CCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	15	0.375	No Hit
CAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	14	0.35000000000000003	No Hit
CCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	14	0.35000000000000003	No Hit
CAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
TAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	12	0.3	No Hit
ACCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
CTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
ATAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
AAAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	11	0.27499999999999997	No Hit
CCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
CAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
ACAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	11	0.27499999999999997	No Hit
TAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	11	0.27499999999999997	No Hit
CCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
ATAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
CCAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	10	0.25	No Hit
TGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
AACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
TGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
AGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
AACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
CGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
AGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CTAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
TGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
TGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
CGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
AGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTA	8	0.2	No Hit
TTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
AAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
CAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
ACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
TGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
ACCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
ATAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
TTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CCCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
CTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
AGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
ATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
CGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
ATAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
ACCGGTACCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGAG	7	0.17500000000000002	Illumina Single End PCR Primer 1 (97% over 34bp)
AGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
ACAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
CACGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
AAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
AGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
TCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TTAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
AAAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAT	6	0.15	No Hit
TGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TGTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CGCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
AAAGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTA	6	0.15	No Hit
AACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TTTGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
AATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TTTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
AAAGGGAGAGTCCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCA	5	0.125	No Hit
TGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AAAGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCT	5	0.125	No Hit
CTCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AAAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGT	5	0.125	No Hit
TATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
ACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	5	0.125	No Hit
CCGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TTAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.2	0.0	0.0	0.0	0.0
11	0.25	0.0	0.0	0.0	0.0
12	0.3	0.0	0.0	0.0	0.0
13	0.45	0.0	0.0	0.0	0.0
14	0.45	0.0	0.0	0.0	0.0
15	0.45	0.0	0.0	0.0	0.0
16	0.475	0.0	0.0	0.0	0.0
17	0.475	0.0	0.0	0.0	0.0
18	0.475	0.0	0.0	0.0	0.0
19	0.55	0.0	0.0	0.0	0.0
20	0.55	0.0	0.0	0.0	0.0
21	0.55	0.0	0.0	0.0	0.0
22	0.55	0.0	0.0	0.0	0.0
23	0.65	0.0	0.0	0.0	0.0
24	0.7	0.0	0.0	0.0	0.0
25	0.775	0.0	0.0	0.0	0.0
26	0.85	0.0	0.0	0.0	0.0
27	0.925	0.0	0.0	0.0	0.0
28	1.0	0.0	0.0	0.0	0.0
29	1.05	0.0	0.0	0.0	0.0
30	1.2	0.0	0.0	0.0	0.0
31	1.325	0.0	0.0	0.0	0.0
32	1.425	0.0	0.0	0.0	0.0
33	1.6	0.0	0.0	0.0	0.0
34	1.6	0.0	0.0	0.0	0.0
35	1.65	0.0	0.0	0.0	0.0
36	1.7	0.0	0.0	0.0	0.0
37	1.725	0.0	0.0	0.0	0.0
38	1.8	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTGCGC	80	0.0	53.303066	44
GTAGTGC	25	2.7296378E-4	52.483017	44
GTGCATG	35	2.3092978E-5	46.859837	44
GGGAGAT	20	7.3862134E-4	43.462498	4
AAAGGGA	30	2.1240267E-6	43.462498	1
CGCTGCG	75	0.0	43.04857	43
CGTAGTG	25	0.001128051	39.73714	43
AAGGGAG	45	1.6796548E-8	38.633335	2
ACGCATC	75	0.0	38.144302	37
ATCGCTG	75	0.0	38.144302	41
CGCATCG	75	0.0	38.144302	38
GCATCGC	75	0.0	38.144302	39
CATCGCT	75	0.0	38.144302	40
GGGAGAC	75	0.0	37.667496	4
AGGGAGA	105	0.0	37.25357	3
TCGCTGC	80	0.0	35.760284	42
ACCGGTT	80	0.0	35.313282	24
TTCATTA	80	0.0	35.313282	15
GGAGACA	80	0.0	35.313282	5
ACACGGT	80	0.0	35.313282	9
>>END_MODULE
Read 552138 spots for SRR23332737.sra
Written 552138 spots for SRR23332737.sra
Read 552134 spots for SRR23332737.sra
Written 552134 spots for SRR23332737.sra
Read 552134 spots for SRR23332737.sra
Written 552134 spots for SRR23332737.sra
Read 552134 spots for SRR23332737.sra
Written 552134 spots for SRR23332737.sra
Read 552134 spots for SRR23332737.sra
Written 552134 spots for SRR23332737.sra
Read 552134 spots for SRR23332737.sra
Written 552134 spots for SRR23332737.sra
Read 552134 spots for SRR23332737.sra
Written 552134 spots for SRR23332737.sra
Read 552134 spots for SRR23332737.sra
Written 552134 spots for SRR23332737.sra
SRR ids: ['SRR23332737.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ngcn2xvc
SRR23332737.sra spots: 4417076
blocks: [[1, 552134], [552135, 1104268], [1104269, 1656402], [1656403, 2208536], [2208537, 2760670], [2760671, 3312804], [3312805, 3864938], [3864939, 4417076]]
SRR23332737 file size 537024
SRR23332737 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR23332737 SRR23332737_1.fastq SRR23332737_2.fastq
Input file:	SRR23332737_1.fastq
Paired file:	SRR23332737_2.fastq
trimmed:	SRR23332737-trimmed-pair1.fastq, SRR23332737-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 03:09:02 2026 >> started

Wed Mar 25 03:09:05 2026 >> done (3.014s)
4417076 read pairs processed; of these:
   2342 ( 0.05%) short read pairs filtered out after trimming by size control
      4 ( 0.00%) empty read pairs filtered out after trimming by size control
4414730 (99.95%) read pairs available; of these:
  36907 ( 0.84%) trimmed read pairs available after processing
4377823 (99.16%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    495	  0.01%
 19	    701	  0.02%
 20	    944	  0.02%
 21	    858	  0.02%
 22	    864	  0.02%
 23	    896	  0.02%
 24	   1071	  0.02%
 25	   1305	  0.03%
 26	   1091	  0.02%
 27	   1056	  0.02%
 28	    884	  0.02%
 29	   1381	  0.03%
 30	   1041	  0.02%
 31	   1301	  0.03%
 32	   1419	  0.03%
 33	   1647	  0.04%
 34	   2467	  0.06%
 35	   2243	  0.05%
 36	   2005	  0.05%
 37	   1740	  0.04%
 38	   2186	  0.05%
 39	   2268	  0.05%
 40	   4451	  0.10%
 41	   6253	  0.14%
 42	 210669	  4.77%
 43	 109822	  2.49%
 44	 483580	 10.95%
 45	3570092	 80.87%
4414730 reads passed initial QC


criterion=sequence-density
sequence-density=1.42
sequence-density-rank=1
fanout-score=2.52
fanout-score-rank=17
prefix-density=1.37
prefix-fanout=2.5
sequence=ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=43
fanout-score=7.25
fanout-score-rank=1
prefix-density=0.02
prefix-fanout=1.8
sequence=TGTGCGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGG


criterion=sequence-density
sequence-density=14.81
sequence-density-rank=1
fanout-score=22.67
fanout-score-rank=3
prefix-density=11.13
prefix-fanout=22.7
sequence=GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCC


criterion=fanout-score
sequence-density=0.54
sequence-density-rank=10
fanout-score=225.68
fanout-score-rank=1
prefix-density=10.60
prefix-fanout=11.5
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTACCGGTACC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG -y GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCC -o SRR23332737 SRR23332737_1.fastq SRR23332737_2.fastq
Input file:	SRR23332737_1.fastq
Paired file:	SRR23332737_2.fastq
trimmed:	SRR23332737-trimmed-pair1.fastq, SRR23332737-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG
-- paired 3' end adapter sequence (-y):	GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 03:09:14 2026 >> started

Wed Mar 25 03:09:16 2026 >> done (2.208s)
3433679 read pairs processed; of these:
  31499 ( 0.92%) short read pairs filtered out after trimming by size control
   5160 ( 0.15%) empty read pairs filtered out after trimming by size control
3397020 (98.93%) read pairs available; of these:
   1711 ( 0.05%) trimmed read pairs available after processing
3395309 (99.95%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    379	  0.01%
 19	    545	  0.02%
 20	    717	  0.02%
 21	    664	  0.02%
 22	    670	  0.02%
 23	    672	  0.02%
 24	    855	  0.03%
 25	   1018	  0.03%
 26	    868	  0.03%
 27	    808	  0.02%
 28	    680	  0.02%
 29	   1094	  0.03%
 30	    795	  0.02%
 31	   1028	  0.03%
 32	   1084	  0.03%
 33	   1270	  0.04%
 34	   1903	  0.06%
 35	   1766	  0.05%
 36	   1557	  0.05%
 37	   1330	  0.04%
 38	   1689	  0.05%
 39	   1762	  0.05%
 40	   3447	  0.10%
 41	   4856	  0.14%
 42	 163104	  4.80%
 43	  85422	  2.51%
 44	 358075	 10.54%
 45	2758962	 81.22%


criterion=sequence-density
sequence-density=1.28
sequence-density-rank=1
fanout-score=2.62
fanout-score-rank=14
prefix-density=1.33
prefix-fanout=2.5
sequence=ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=43
fanout-score=7.89
fanout-score-rank=1
prefix-density=0.02
prefix-fanout=1.8
sequence=TGTGCGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGG


criterion=sequence-density
sequence-density=14.16
sequence-density-rank=1
fanout-score=23.16
fanout-score-rank=3
prefix-density=11.00
prefix-fanout=23.2
sequence=GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGGACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCTCACGGTT


criterion=fanout-score
sequence-density=0.54
sequence-density-rank=10
fanout-score=226.38
fanout-score-rank=1
prefix-density=10.69
prefix-fanout=11.4
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTACCGGTACC
SRR23332737 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 25 03:09:33
                             Started mapping on |	Mar 25 03:09:33
                                    Finished on |	Mar 25 03:12:04
       Mapping speed, Million of reads per hour |	104.38

                          Number of input reads |	4378071
                      Average input read length |	90
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2182315
                        Uniquely mapped reads % |	49.85%
                          Average mapped length |	87.77
                       Number of splices: Total |	137587
            Number of splices: Annotated (sjdb) |	473
                       Number of splices: GT/AG |	125368
                       Number of splices: GC/AG |	2934
                       Number of splices: AT/AC |	454
               Number of splices: Non-canonical |	8831
                      Mismatch rate per base, % |	0.29%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.30
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1024322
             % of reads mapped to multiple loci |	23.40%
        Number of reads mapped to too many loci |	832301
             % of reads mapped to too many loci |	19.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.40%
                     % of reads unmapped: other |	0.35%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1171435	1171435	1171435
N_multimapping	1024322	1024322	1024322
N_noFeature	61728	1999319	144198
N_ambiguous	295798	36629	166168
UnstrandedReadsAssigned:1824789 PositiveStrandReadsAssigned:146367 NegativeStrandReadsAssigned:1871949
Dataset is classified negative stranded
MeadianReadLen=41 20thPercentileLength=41 echo kmer=37
SRR23332737 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR23332737-trimmed-pair1.fastq
                             SRR23332737-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,378,071 reads, 962,099 reads pseudoaligned
[quant] estimated average fragment length: 205.229
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 52 rounds

  4323 SRR23332737.ke.tsv
  4497 SRR23332737.se.tsv
  8820 total
==> SRR23332737.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	12.542	72	1594.34
AAC73113	2463	2258.77	157	19.3038
AAC73114	933	728.799	68	25.9129
AAC73115	1287	1082.77	162	41.5521
AAC73116	297	118.832	21	49.0795
AAC73117	777	572.971	36	17.4496
AAC73118	1431	1226.77	21	4.75412
AAC73119	954	749.78	2268	840.085
AAC73120	588	385.166	212	152.863

==> SRR23332737.se.tsv <==
b0001	122
b0002	153
b0003	52
b0004	151
b0005	22
b0006	58
b0007	30
b0008	2265
b0009	231
b0010	15
SRR23332737 completed mapping pipeline successfully
