Starting /dee2/code/volunteer_pipeline.sh SRR23332723
    current disk space = 1461689532
    free memory = 58305604 
SRR23332723 SRAfilesize
d19a9fcaf72c408670f094367ec6e286  SRR23332723.sra
SRR23332723.sra file validated
SRR23332723 is paired end
SRR23332723 is conventional basespace
SRR23332723 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332723_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.4875	37.0	37.0	37.0	37.0	37.0
2	36.5155	37.0	37.0	37.0	37.0	37.0
3	36.405	37.0	37.0	37.0	37.0	37.0
4	36.3925	37.0	37.0	37.0	37.0	37.0
5	36.396	37.0	37.0	37.0	37.0	37.0
6	36.379	37.0	37.0	37.0	37.0	37.0
7	36.3485	37.0	37.0	37.0	37.0	37.0
8	36.44	37.0	37.0	37.0	37.0	37.0
9	36.5275	37.0	37.0	37.0	37.0	37.0
10	36.502	37.0	37.0	37.0	37.0	37.0
11	36.388	37.0	37.0	37.0	37.0	37.0
12	36.491	37.0	37.0	37.0	37.0	37.0
13	36.4995	37.0	37.0	37.0	37.0	37.0
14	36.3685	37.0	37.0	37.0	37.0	37.0
15	36.507	37.0	37.0	37.0	37.0	37.0
16	36.4785	37.0	37.0	37.0	37.0	37.0
17	36.293	37.0	37.0	37.0	37.0	37.0
18	36.252	37.0	37.0	37.0	37.0	37.0
19	36.346	37.0	37.0	37.0	37.0	37.0
20	36.4085	37.0	37.0	37.0	37.0	37.0
21	36.387	37.0	37.0	37.0	37.0	37.0
22	36.298	37.0	37.0	37.0	37.0	37.0
23	36.317	37.0	37.0	37.0	37.0	37.0
24	36.391	37.0	37.0	37.0	37.0	37.0
25	36.43	37.0	37.0	37.0	37.0	37.0
26	36.2195	37.0	37.0	37.0	37.0	37.0
27	36.288	37.0	37.0	37.0	37.0	37.0
28	36.432	37.0	37.0	37.0	37.0	37.0
29	36.2395	37.0	37.0	37.0	37.0	37.0
30	36.203	37.0	37.0	37.0	37.0	37.0
31	36.374	37.0	37.0	37.0	37.0	37.0
32	36.298	37.0	37.0	37.0	37.0	37.0
33	36.3545	37.0	37.0	37.0	37.0	37.0
34	36.259	37.0	37.0	37.0	37.0	37.0
35	36.329	37.0	37.0	37.0	37.0	37.0
36	36.05	37.0	37.0	37.0	37.0	37.0
37	36.2915	37.0	37.0	37.0	37.0	37.0
38	36.333	37.0	37.0	37.0	37.0	37.0
39	36.227	37.0	37.0	37.0	37.0	37.0
40	36.3075	37.0	37.0	37.0	37.0	37.0
41	36.1425	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	0.0
24	1.0
25	3.0
26	6.0
27	9.0
28	9.0
29	12.0
30	28.0
31	43.0
32	63.0
33	79.0
34	111.0
35	264.0
36	1225.0
37	2146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.775	18.5	45.625	9.1
2	32.475	18.775	28.025	20.724999999999998
3	33.275	19.8	27.1	19.825
4	20.4	23.150000000000002	36.125	20.325
5	34.725	23.674999999999997	21.05	20.549999999999997
6	35.75	24.075	17.599999999999998	22.575
7	21.4	35.475	18.05	25.074999999999996
8	37.5	17.175	19.775000000000002	25.55
9	24.325	13.775	22.25	39.65
10	24.325	14.499999999999998	34.675	26.5
11	38.1	17.424999999999997	19.225	25.25
12	38.625	18.45	20.0	22.925
13	24.125	19.875	18.6	37.4
14	38.025	20.599999999999998	20.175	21.2
15	38.550000000000004	21.099999999999998	20.75	19.6
16	39.975	22.025	19.25	18.75
17	25.35	33.625	22.8	18.224999999999998
18	38.75	19.8	22.725	18.725
19	25.1	19.6	36.175000000000004	19.125
20	24.4	20.575	34.775	20.25
21	39.300000000000004	20.8	18.975	20.925
22	23.974999999999998	35.775	18.95	21.3
23	24.925	33.675	19.3	22.1
24	25.924999999999997	18.85	19.1	36.125
25	25.25	17.325	20.275000000000002	37.15
26	22.825	18.55	22.85	35.775
27	38.85	19.375	19.375	22.400000000000002
28	22.95	17.625	23.625	35.8
29	38.45	18.975	23.45	19.125
30	22.925	17.849999999999998	39.625	19.6
31	22.825	19.85	22.375	34.949999999999996
32	23.025000000000002	20.125	22.75	34.1
33	36.7	22.225	20.849999999999998	20.225
34	21.224999999999998	37.3	21.2	20.275000000000002
35	21.875	37.75	20.7	19.675
36	22.2	37.05	19.975	20.775
37	22.3	21.75	34.775	21.175
38	23.875	20.875	34.8	20.45
39	22.15	21.099999999999998	19.900000000000002	36.85
40	20.75	21.75	20.549999999999997	36.95
41	20.925	22.05	34.975	22.05
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	1.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	1.0
20	1.0
21	1.0
22	1.0
23	1.5
24	2.0
25	2.0
26	2.0
27	2.0
28	9.0
29	16.0
30	15.0
31	14.0
32	14.0
33	22.0
34	30.0
35	33.0
36	36.0
37	36.0
38	59.0
39	82.0
40	109.0
41	136.0
42	136.0
43	191.5
44	247.0
45	304.5
46	362.0
47	362.0
48	381.0
49	400.0
50	434.0
51	468.0
52	479.0
53	490.0
54	490.0
55	442.5
56	395.0
57	337.0
58	279.0
59	279.0
60	563.0
61	847.0
62	473.5
63	100.0
64	100.0
65	74.0
66	48.0
67	36.5
68	25.0
69	25.0
70	19.0
71	13.0
72	7.5
73	2.0
74	1.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.5
80	1.0
81	1.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.525000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.53096967108073	45.375
2	13.925672789406237	16.3
3	4.48526270824434	7.875
4	1.751388295600171	4.1000000000000005
5	0.7689021785561726	2.25
6	0.5126014523707817	1.7999999999999998
7	0.34173430158052115	1.4000000000000001
8	0.08543357539513029	0.4
9	0.0	0.0
>10	0.5553182400683468	5.8500000000000005
>50	0.0	0.0
>100	0.0	0.0
>500	0.042716787697565144	14.649999999999999
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCT	586	14.649999999999999	No Hit
GGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTA	46	1.15	No Hit
GCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACA	34	0.8500000000000001	No Hit
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTAC	29	0.7250000000000001	No Hit
AAAAAACGCGCCCGAAGGCGCGTTGGCGATACACTCAATAT	19	0.475	No Hit
GGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAAC	15	0.375	No Hit
AAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAA	14	0.35000000000000003	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCC	13	0.325	No Hit
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTC	12	0.3	TruSeq Adapter, Index 9 (97% over 37bp)
AGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCCA	12	0.3	No Hit
GGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTC	10	0.25	No Hit
TTCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	10	0.25	No Hit
GTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTT	10	0.25	No Hit
TGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACA	10	0.25	No Hit
TTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCAC	8	0.2	No Hit
GATCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCC	8	0.2	No Hit
ATCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGAC	7	0.17500000000000002	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACGAGAGGACCGGGA	7	0.17500000000000002	No Hit
AAAAAATGGCGCACAATGTGCGCCATTTTTCACTTCACAGG	7	0.17500000000000002	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCC	7	0.17500000000000002	No Hit
TGTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCT	7	0.17500000000000002	No Hit
AGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCCC	7	0.17500000000000002	No Hit
ATGAAGGGGGAGACGCTTTCTCCCCCTTAGTTTTCAGGCCT	7	0.17500000000000002	No Hit
TGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	7	0.17500000000000002	No Hit
GGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	6	0.15	No Hit
TTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGACTCCTT	6	0.15	No Hit
GCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACTAC	6	0.15	No Hit
TTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACT	6	0.15	No Hit
TTTCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTCC	6	0.15	No Hit
AGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGT	6	0.15	No Hit
GGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACAT	6	0.15	No Hit
TTATCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTG	6	0.15	No Hit
GTTTCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTC	6	0.15	No Hit
TCTCGTACTAGGAGTGGACGCATCACTGGTGTTCGGGTTGT	6	0.15	No Hit
TCAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTAC	6	0.15	No Hit
TTATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGAA	6	0.15	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAAC	5	0.125	No Hit
GGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAACC	5	0.125	No Hit
AAAAAAATGGCGCACAATGTGCGCCATTTTTCACTTCACAG	5	0.125	No Hit
ACACGCCACTAACAAACTAGCCTGATTAAGTTTTAACGCTT	5	0.125	No Hit
GTCCTAAGAGCGGAGGCTAGGGAGAGAGGGCTCTAAGCAGG	5	0.125	No Hit
CCGACTTCATGGAGTCGAGTTGCAGAAGAAGTAGGTAGCTT	5	0.125	No Hit
GACTCTCAAGGAACGTTGAAGACGACGACGTTGATAGGCCG	5	0.125	No Hit
TATGAGGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCC	5	0.125	No Hit
AGGTTAAGCCTCACGGTTCATTAGTACGAGAGGACCGGGAT	5	0.125	No Hit
ACGCCACTAACAAACTAGCCTGATTAAGTTTTAACGCTTCA	5	0.125	No Hit
ATCAACGGCTCCTGAATCAGGAGCCGTCTTATTACTGCATA	5	0.125	No Hit
CTTATCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCT	5	0.125	No Hit
GGGTTTGACCTCTCTTGATCCCCGTCCTAAGAGCGGAGGCT	5	0.125	No Hit
ACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTAC	5	0.125	No Hit
GCGTTGAGCTAACCGGTACTAATGAACCGTGTCTCCCCCCA	5	0.125	No Hit
TATCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGA	5	0.125	No Hit
TTCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTCCT	5	0.125	No Hit
TTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGTCGT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.025	0.0	0.0	0.0	0.0
2	0.025	0.0	0.0	0.0	0.0
3	0.025	0.0	0.0	0.0	0.0
4	0.325	0.0	0.0	0.0	0.0
5	0.425	0.0	0.0	0.0	0.0
6	0.45	0.0	0.0	0.0	0.0
7	0.45	0.0	0.0	0.0	0.0
8	0.45	0.0	0.0	0.0	0.0
9	0.475	0.0	0.0	0.0	0.0
10	0.475	0.0	0.0	0.0	0.0
11	0.475	0.0	0.0	0.0	0.0
12	0.5	0.0	0.0	0.0	0.0
13	0.55	0.0	0.0	0.0	0.0
14	0.55	0.0	0.0	0.0	0.0
15	0.6	0.0	0.0	0.0	0.0
16	0.6	0.0	0.0	0.0	0.0
17	0.625	0.0	0.0	0.0	0.0
18	0.625	0.0	0.0	0.0	0.0
19	0.675	0.0	0.0	0.0	0.0
20	0.75	0.0	0.0	0.0	0.0
21	0.825	0.0	0.0	0.0	0.0
22	0.85	0.0	0.0	0.0	0.0
23	0.85	0.0	0.0	0.0	0.0
24	0.875	0.0	0.0	0.0	0.0
25	0.9	0.0	0.0	0.0	0.0
26	0.925	0.0	0.0	0.0	0.0
27	1.025	0.0	0.0	0.0	0.0
28	1.125	0.0	0.0	0.0	0.0
29	1.175	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTGGAG	40	4.0159648E-8	35.0	2
TGGTGGA	40	4.0159648E-8	35.0	1
GCGGGAG	45	1.1330303E-7	31.111113	12
GTTGAAC	45	1.1330303E-7	31.111113	18
GAAATTC	45	1.1330303E-7	31.111113	33
AAATTCC	45	1.1330303E-7	31.111113	34
AATTCCT	45	1.1330303E-7	31.111113	35
GTCCGAA	45	1.1330303E-7	31.111113	29
GCTGGCG	45	1.1330303E-7	31.111113	8
AACCCGC	45	1.1330303E-7	31.111113	22
ACCCGCG	45	1.1330303E-7	31.111113	23
TGGAGCT	45	1.1330303E-7	31.111113	4
GGGAGTT	45	1.1330303E-7	31.111113	14
GAGTTGA	45	1.1330303E-7	31.111113	16
CTGGCGG	45	1.1330303E-7	31.111113	9
CCCGCGT	45	1.1330303E-7	31.111113	24
GAACCCG	45	1.1330303E-7	31.111113	21
CGGGAGT	45	1.1330303E-7	31.111113	13
GCGTCCG	45	1.1330303E-7	31.111113	27
CGAAATT	45	1.1330303E-7	31.111113	32
>>END_MODULE
SRR23332723 read2 length is 40-50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332723_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	40-50
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.484	11.0	11.0	37.0	11.0	37.0
2	22.305	25.0	11.0	37.0	11.0	37.0
3	25.0455	25.0	11.0	37.0	11.0	37.0
4	36.074	37.0	37.0	37.0	37.0	37.0
5	35.9575	37.0	37.0	37.0	37.0	37.0
6	35.942	37.0	37.0	37.0	37.0	37.0
7	35.85	37.0	37.0	37.0	37.0	37.0
8	35.8655	37.0	37.0	37.0	37.0	37.0
9	35.8875	37.0	37.0	37.0	37.0	37.0
10	35.9925	37.0	37.0	37.0	37.0	37.0
11	35.966	37.0	37.0	37.0	37.0	37.0
12	35.945	37.0	37.0	37.0	37.0	37.0
13	36.0255	37.0	37.0	37.0	37.0	37.0
14	35.978	37.0	37.0	37.0	37.0	37.0
15	35.739	37.0	37.0	37.0	37.0	37.0
16	36.088	37.0	37.0	37.0	37.0	37.0
17	36.0435	37.0	37.0	37.0	37.0	37.0
18	36.1245	37.0	37.0	37.0	37.0	37.0
19	36.113	37.0	37.0	37.0	37.0	37.0
20	36.0095	37.0	37.0	37.0	37.0	37.0
21	36.0865	37.0	37.0	37.0	37.0	37.0
22	36.1965	37.0	37.0	37.0	37.0	37.0
23	36.1565	37.0	37.0	37.0	37.0	37.0
24	36.172	37.0	37.0	37.0	37.0	37.0
25	35.979	37.0	37.0	37.0	37.0	37.0
26	36.01325	37.0	37.0	37.0	37.0	37.0
27	36.0465	37.0	37.0	37.0	37.0	37.0
28	36.1175	37.0	37.0	37.0	37.0	37.0
29	36.07	37.0	37.0	37.0	37.0	37.0
30	36.0345	37.0	37.0	37.0	37.0	37.0
31	36.053	37.0	37.0	37.0	37.0	37.0
32	35.9545	37.0	37.0	37.0	37.0	37.0
33	35.9345	37.0	37.0	37.0	37.0	37.0
34	36.1105	37.0	37.0	37.0	37.0	37.0
35	36.1355	37.0	37.0	37.0	37.0	37.0
36	36.18725	37.0	37.0	37.0	37.0	37.0
37	36.11	37.0	37.0	37.0	37.0	37.0
38	35.933	37.0	37.0	37.0	37.0	37.0
39	36.0115	37.0	37.0	37.0	37.0	37.0
40	36.0945	37.0	37.0	37.0	37.0	37.0
41	35.77569392348087	37.0	37.0	37.0	37.0	37.0
42	35.943971985992995	37.0	37.0	37.0	37.0	37.0
43	35.88660826032541	37.0	37.0	37.0	37.0	37.0
44	36.064516129032256	37.0	37.0	37.0	37.0	37.0
45	35.95130890052356	37.0	37.0	37.0	37.0	37.0
46	35.9459815546772	37.0	37.0	37.0	37.0	37.0
47	35.98696114954763	37.0	37.0	37.0	37.0	37.0
48	35.97354211663067	37.0	37.0	37.0	37.0	37.0
49	35.98155985489722	37.0	37.0	37.0	37.0	37.0
50	35.85908927931158	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	4.0
18	0.0
19	4.0
20	6.0
21	5.0
22	8.0
23	9.0
24	11.0
25	12.0
26	15.0
27	16.0
28	26.0
29	23.0
30	40.0
31	60.0
32	89.0
33	156.0
34	364.0
35	1790.0
36	1189.0
37	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	40.625	28.575	30.8
2	34.35	39.574999999999996	11.05	15.024999999999999
3	27.500000000000004	42.025	16.325	14.149999999999999
4	81.325	6.9750000000000005	5.1499999999999995	6.550000000000001
5	67.07499999999999	16.85	9.825000000000001	6.25
6	59.9	20.0	8.15	11.95
7	37.375	42.225	9.15	11.25
8	44.65	19.375	12.525	23.45
9	20.5	39.6	28.000000000000004	11.899999999999999
10	40.675	22.3	17.675	19.35
11	26.924999999999997	41.6	16.45	15.024999999999999
12	21.425	26.450000000000003	26.950000000000003	25.174999999999997
13	37.05	13.725000000000001	29.549999999999997	19.675
14	35.625	15.2	25.275	23.9
15	26.75	18.075	44.375	10.8
16	47.175	20.05	22.325	10.45
17	26.5	32.95	18.375	22.175
18	14.799999999999999	41.475	27.750000000000004	15.975
19	20.1	19.125	34.725	26.05
20	15.35	22.1	34.050000000000004	28.499999999999996
21	17.925	43.7	12.1	26.275
22	41.699999999999996	28.425	12.225	17.65
23	24.55	30.525000000000002	18.675	26.25
24	24.075	22.175	12.525	41.225
25	39.975	17.2	11.600000000000001	31.225
26	32.958239559889975	21.555388847211805	12.703175793948487	32.78319579894974
27	40.5	24.2	20.95	14.35
28	33.991995997999	25.63781890945473	27.063531765882942	13.306653326663332
29	23.75	14.975	48.1	13.175
30	19.684842421210604	15.282641320660332	52.22611305652827	12.8064032016008
31	16.725	23.150000000000002	41.05	19.075
32	35.1175587793897	13.281640820410203	20.485242621310658	31.115557778889446
33	22.0	14.025000000000002	13.900000000000002	50.075
34	26.013006503251624	16.23311655827914	25.162581290645324	32.59129564782391
35	11.4	35.725	23.05	29.825000000000003
36	11.727931982995749	40.735183795948984	29.182295573893473	18.35458864716179
37	16.25	47.425	12.4	23.925
38	21.325	18.625	10.0	50.05
39	36.075	17.675	11.700000000000001	34.55
40	22.1	20.0	18.6	39.300000000000004
41	15.778944736184048	43.11077769442361	25.156289072268066	15.95398849712428
42	20.185092546273136	29.589794897448723	28.31415707853927	21.91095547773887
43	32.515644555694614	22.377972465581976	14.843554443053817	30.262828535669588
44	42.1875	13.886088709677418	18.094758064516128	25.83165322580645
45	17.277486910994764	15.706806282722512	36.30890052356021	30.706806282722514
46	31.225296442687743	18.76152832674572	31.857707509881422	18.155467720685113
47	31.062017567207878	16.23635879691243	24.1149853606601	28.586638275219588
48	27.024838012958963	25.7829373650108	25.404967602591793	21.787257019438442
49	30.964620501965527	12.065315996371334	42.15300876927729	14.817054732385849
50	32.054499820724274	13.660810326281823	25.779849408390103	28.5048404446038
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	1.5
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.5
22	3.0
23	2.5
24	2.0
25	3.0
26	4.0
27	4.5
28	6.0
29	11.0
30	16.5
31	26.0
32	36.5
33	44.5
34	52.0
35	60.0
36	67.5
37	74.0
38	84.0
39	84.5
40	79.5
41	85.0
42	97.5
43	113.0
44	119.5
45	109.0
46	101.5
47	126.0
48	151.0
49	168.0
50	195.5
51	379.5
52	563.5
53	717.0
54	894.5
55	912.5
56	843.0
57	616.5
58	418.0
59	298.5
60	179.5
61	113.5
62	67.5
63	50.0
64	34.5
65	27.5
66	17.0
67	9.0
68	6.0
69	6.0
70	6.0
71	4.0
72	2.0
73	2.5
74	3.0
75	2.0
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.5
82	1.0
83	2.0
84	3.0
85	1.5
86	0.0
87	1.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	2.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.025
27	0.0
28	0.05
29	0.0
30	0.05
31	0.0
32	0.05
33	0.0
34	0.05
35	0.0
36	0.025
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.026609898882384245
48	0.0
49	0.03022974607013301
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
40	1.0
41	1.0
42	3.0
43	27.0
44	148.0
45	25.0
46	37.0
47	54.0
48	396.0
49	519.0
50	2789.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.13733905579399	50.175000000000004
2	4.763948497854077	5.55
3	2.1030042918454934	3.675
4	1.2875536480686696	3.0
5	1.3304721030042919	3.875
6	0.8154506437768241	2.85
7	0.6008583690987125	2.45
8	0.4291845493562232	2.0
9	0.5150214592274678	2.7
>10	1.888412017167382	18.55
>50	0.12875536480686695	5.175
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	95	2.375	No Hit
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	56	1.4000000000000001	No Hit
AAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	56	1.4000000000000001	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	42	1.05	No Hit
AAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	42	1.05	No Hit
CAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	37	0.9249999999999999	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	26	0.65	No Hit
TAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	25	0.625	No Hit
AATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	24	0.6	No Hit
CAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	24	0.6	No Hit
CAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	23	0.575	No Hit
CGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	22	0.5499999999999999	No Hit
TGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	22	0.5499999999999999	No Hit
CGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	18	0.44999999999999996	No Hit
CGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	18	0.44999999999999996	No Hit
AGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	18	0.44999999999999996	No Hit
AATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	17	0.42500000000000004	No Hit
AACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	17	0.42500000000000004	No Hit
TAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	17	0.42500000000000004	No Hit
ATAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	17	0.42500000000000004	No Hit
CACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
CCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
ACAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
CGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	15	0.375	No Hit
TGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	14	0.35000000000000003	No Hit
TACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
AGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
CAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
AGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
TAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	13	0.325	No Hit
TAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	12	0.3	No Hit
CGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
TATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
CCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
CACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
CGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	11	0.27499999999999997	No Hit
TGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	11	0.27499999999999997	No Hit
TGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	11	0.27499999999999997	No Hit
CGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
CGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
CTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
AATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
AAAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	9	0.22499999999999998	No Hit
TCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
ACCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
CGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	9	0.22499999999999998	No Hit
ACAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
CGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
CGCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
AAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
CCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
TGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
TGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
TGGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
CCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
AGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
TACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
TGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
TCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
AAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
AAAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAT	7	0.17500000000000002	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTA	7	0.17500000000000002	No Hit
CCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TGTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
CAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
AAAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGT	7	0.17500000000000002	No Hit
TATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TTTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
ACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
ACAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
TAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AGAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
AGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AAAGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCT	6	0.15	No Hit
TAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
ATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CCAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
AGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TTGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
AACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TCTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
ACAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CGAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
AGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CACGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
AGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
CTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TGTGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TCGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CCCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
AGACACGGTACTAATGAACCGTGAGGCTTAACCTATCCCGCGGA	5	0.125	No Hit
TCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
AAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CGTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
ACTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AAAGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTA	5	0.125	No Hit
CTCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TGCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
AAAGTGATTGCACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCA	5	0.125	No Hit
CCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CATGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TTTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AACGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
ACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
AGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TTTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.1	0.0	0.0	0.0	0.0
11	0.15	0.0	0.0	0.0	0.0
12	0.225	0.0	0.0	0.0	0.0
13	0.425	0.0	0.0	0.0	0.0
14	0.475	0.0	0.0	0.0	0.0
15	0.5	0.0	0.0	0.0	0.0
16	0.5	0.0	0.0	0.0	0.0
17	0.5	0.0	0.0	0.0	0.0
18	0.5	0.0	0.0	0.0	0.0
19	0.5	0.0	0.0	0.0	0.0
20	0.55	0.0	0.0	0.0	0.0
21	0.55	0.0	0.0	0.0	0.0
22	0.65	0.0	0.0	0.0	0.0
23	0.65	0.0	0.0	0.0	0.0
24	0.65	0.0	0.0	0.0	0.0
25	0.675	0.0	0.0	0.0	0.0
26	0.7	0.0	0.0	0.0	0.0
27	0.775	0.0	0.0	0.0	0.0
28	0.85	0.0	0.0	0.0	0.0
29	0.95	0.0	0.0	0.0	0.0
30	1.0	0.0	0.0	0.0	0.0
31	1.025	0.0	0.0	0.0	0.0
32	1.025	0.0	0.0	0.0	0.0
33	1.05	0.0	0.0	0.0	0.0
34	1.05	0.0	0.0	0.0	0.0
35	1.075	0.0	0.0	0.0	0.0
36	1.1	0.0	0.0	0.0	0.0
37	1.2	0.0	0.0	0.0	0.0
38	1.275	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCATG	65	0.0	59.333336	44
GGTGCAT	65	0.0	47.820896	43
AAAGGGA	40	6.017217E-9	43.3875	1
TGGGAGA	20	7.4493483E-4	43.3875	3
GCTGCGC	30	7.452275E-4	42.851852	44
AGGTGCA	65	0.0	42.72	42
AAGGTGC	65	0.0	41.61039	41
CAAGGTG	65	0.0	40.556965	40
CCAAGGT	65	0.0	40.556965	39
AGGGAGA	75	0.0	40.495	3
TCGACGG	65	0.0	40.050003	20
TCTGGAT	65	0.0	40.050003	13
TGATTCT	65	0.0	40.050003	9
GACGGGA	65	0.0	40.050003	22
GGATTTG	65	0.0	40.050003	26
TTCGACG	65	0.0	40.050003	19
TGCGAAA	65	0.0	40.050003	31
GGATTCG	65	0.0	40.050003	16
GCGAAAC	65	0.0	40.050003	32
CTGATTC	65	0.0	40.050003	8
>>END_MODULE
Read 655787 spots for SRR23332723.sra
Written 655787 spots for SRR23332723.sra
Read 655787 spots for SRR23332723.sra
Written 655787 spots for SRR23332723.sra
Read 655787 spots for SRR23332723.sra
Written 655787 spots for SRR23332723.sra
Read 655787 spots for SRR23332723.sra
Written 655787 spots for SRR23332723.sra
Read 655787 spots for SRR23332723.sra
Written 655787 spots for SRR23332723.sra
Read 655787 spots for SRR23332723.sra
Written 655787 spots for SRR23332723.sra
Read 655787 spots for SRR23332723.sra
Written 655787 spots for SRR23332723.sra
Read 655787 spots for SRR23332723.sra
Written 655787 spots for SRR23332723.sra
SRR ids: ['SRR23332723.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_bqiufwul
SRR23332723.sra spots: 5246296
blocks: [[1, 655787], [655788, 1311574], [1311575, 1967361], [1967362, 2623148], [2623149, 3278935], [3278936, 3934722], [3934723, 4590509], [4590510, 5246296]]
SRR23332723 file size 638248
SRR23332723 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR23332723 SRR23332723_1.fastq SRR23332723_2.fastq
Input file:	SRR23332723_1.fastq
Paired file:	SRR23332723_2.fastq
trimmed:	SRR23332723-trimmed-pair1.fastq, SRR23332723-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 03:35:16 2026 >> started

Wed Mar 25 03:35:19 2026 >> done (3.122s)
5246296 read pairs processed; of these:
    989 ( 0.02%) short read pairs filtered out after trimming by size control
    150 ( 0.00%) empty read pairs filtered out after trimming by size control
5245157 (99.98%) read pairs available; of these:
  39495 ( 0.75%) trimmed read pairs available after processing
5205662 (99.25%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    322	  0.01%
 19	    349	  0.01%
 20	    468	  0.01%
 21	    457	  0.01%
 22	    448	  0.01%
 23	    450	  0.01%
 24	    685	  0.01%
 25	    952	  0.02%
 26	    730	  0.01%
 27	    841	  0.02%
 28	    652	  0.01%
 29	   2753	  0.05%
 30	    848	  0.02%
 31	   1031	  0.02%
 32	   1118	  0.02%
 33	   1595	  0.03%
 34	   1973	  0.04%
 35	   1912	  0.04%
 36	   1546	  0.03%
 37	   1772	  0.03%
 38	   2695	  0.05%
 39	   3004	  0.06%
 40	   9229	  0.18%
 41	   5975	  0.11%
 42	 248786	  4.74%
 43	  99742	  1.90%
 44	 467376	  8.91%
 45	4387448	 83.65%
5245157 reads passed initial QC


criterion=sequence-density
sequence-density=1.35
sequence-density-rank=1
fanout-score=1.96
fanout-score-rank=27
prefix-density=1.20
prefix-fanout=2.0
sequence=GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=44
fanout-score=27.84
fanout-score-rank=1
prefix-density=0.12
prefix-fanout=1.2
sequence=TTTTTTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACAGAATCAGGCCATTACTACCCTGTCCGCCATGGCTTCTCGCCAGCCTCCCAGCCATTGTGACCTTTGATTCAGCGTCTGATAGGGACACATTTCTTTTGAGCGTCCGGCGATGCCGGCCTGATAACCACGTTGATGTGCCCGTTCCAGGCGATCTCGTTTTTGTCTCTTCATGCCTCGTTTCCCTCATACTGG


criterion=sequence-density
sequence-density=16.37
sequence-density-rank=1
fanout-score=17.91
fanout-score-rank=7
prefix-density=14.04
prefix-fanout=17.9
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCC


criterion=fanout-score
sequence-density=0.63
sequence-density-rank=11
fanout-score=201.60
fanout-score-rank=1
prefix-density=11.12
prefix-fanout=11.4
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTATCCCGCGG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCG -y GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCC -o SRR23332723 SRR23332723_1.fastq SRR23332723_2.fastq
Input file:	SRR23332723_1.fastq
Paired file:	SRR23332723_2.fastq
trimmed:	SRR23332723-trimmed-pair1.fastq, SRR23332723-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAG
-- paired 3' end adapter sequence (-y):	GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 03:35:29 2026 >> started

Wed Mar 25 03:35:31 2026 >> done (2.780s)
4196126 read pairs processed; of these:
  36510 ( 0.87%) short read pairs filtered out after trimming by size control
   4421 ( 0.11%) empty read pairs filtered out after trimming by size control
4155195 (99.02%) read pairs available; of these:
   1514 ( 0.04%) trimmed read pairs available after processing
4153681 (99.96%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    250	  0.01%
 19	    282	  0.01%
 20	    374	  0.01%
 21	    367	  0.01%
 22	    356	  0.01%
 23	    337	  0.01%
 24	    556	  0.01%
 25	    749	  0.02%
 26	    601	  0.01%
 27	    653	  0.02%
 28	    530	  0.01%
 29	   2219	  0.05%
 30	    677	  0.02%
 31	    830	  0.02%
 32	    882	  0.02%
 33	   1286	  0.03%
 34	   1595	  0.04%
 35	   1542	  0.04%
 36	   1224	  0.03%
 37	   1431	  0.03%
 38	   2125	  0.05%
 39	   2411	  0.06%
 40	   7399	  0.18%
 41	   4752	  0.11%
 42	 198653	  4.78%
 43	  79784	  1.92%
 44	 362734	  8.73%
 45	3480596	 83.76%


criterion=sequence-density
sequence-density=1.32
sequence-density-rank=1
fanout-score=2.55
fanout-score-rank=19
prefix-density=1.34
prefix-fanout=2.5
sequence=ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=39
fanout-score=15.41
fanout-score-rank=1
prefix-density=0.06
prefix-fanout=3.0
sequence=CTTGATTTTCCGGATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACTACGGTGCTGAAGCAACAAATGCCCTGCTTCCAGGAATCTCCCCCCAG


criterion=sequence-density
sequence-density=15.70
sequence-density-rank=1
fanout-score=17.39
fanout-score-rank=7
prefix-density=13.67
prefix-fanout=17.4
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCC


criterion=fanout-score
sequence-density=0.63
sequence-density-rank=13
fanout-score=201.41
fanout-score-rank=1
prefix-density=11.15
prefix-fanout=11.4
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTATCCCGCGG
SRR23332723 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 25 03:35:48
                             Started mapping on |	Mar 25 03:35:48
                                    Finished on |	Mar 25 03:38:26
       Mapping speed, Million of reads per hour |	118.58

                          Number of input reads |	5204226
                      Average input read length |	90
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2707607
                        Uniquely mapped reads % |	52.03%
                          Average mapped length |	87.75
                       Number of splices: Total |	166758
            Number of splices: Annotated (sjdb) |	143
                       Number of splices: GT/AG |	153595
                       Number of splices: GC/AG |	3260
                       Number of splices: AT/AC |	405
               Number of splices: Non-canonical |	9498
                      Mismatch rate per base, % |	0.28%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.32
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1102447
             % of reads mapped to multiple loci |	21.18%
        Number of reads mapped to too many loci |	1063137
             % of reads mapped to too many loci |	20.43%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.02%
                     % of reads unmapped: other |	0.35%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1394176	1394176	1394176
N_multimapping	1102447	1102447	1102447
N_noFeature	98666	2436054	197368
N_ambiguous	269656	40080	69485
UnstrandedReadsAssigned:2339285 PositiveStrandReadsAssigned:231473 NegativeStrandReadsAssigned:2440754
Dataset is classified negative stranded
MeadianReadLen=41 20thPercentileLength=41 echo kmer=37
SRR23332723 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR23332723-trimmed-pair1.fastq
                             SRR23332723-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 5,204,226 reads, 918,324 reads pseudoaligned
[quant] estimated average fragment length: 208.191
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 151 rounds

  4323 SRR23332723.ke.tsv
  4497 SRR23332723.se.tsv
  8820 total
==> SRR23332723.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	12.6492	313	3471.42
AAC73113	2463	2255.81	90	5.59713
AAC73114	933	725.825	43	8.31117
AAC73115	1287	1079.81	80	10.3937
AAC73116	297	117.563	2	2.38662
AAC73117	777	569.993	25	6.15312
AAC73118	1431	1223.81	56	6.41948
AAC73119	954	746.809	205	38.5097
AAC73120	588	382.412	62	22.745

==> SRR23332723.se.tsv <==
b0001	479
b0002	79
b0003	38
b0004	75
b0005	4
b0006	28
b0007	64
b0008	207
b0009	66
b0010	31
SRR23332723 completed mapping pipeline successfully
