Starting /dee2/code/volunteer_pipeline.sh SRR23332654
    current disk space = 1435080684
    free memory = 57541572 
SRR23332654 SRAfilesize
2a97875c6c1de70416dc42a91a6176b8  SRR23332654.sra
SRR23332654.sra file validated
SRR23332654 is paired end
SRR23332654 is conventional basespace
SRR23332654 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332654_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.58	37.0	37.0	37.0	37.0	37.0
2	36.4695	37.0	37.0	37.0	37.0	37.0
3	36.535	37.0	37.0	37.0	37.0	37.0
4	36.506	37.0	37.0	37.0	37.0	37.0
5	36.484	37.0	37.0	37.0	37.0	37.0
6	36.324	37.0	37.0	37.0	37.0	37.0
7	36.5255	37.0	37.0	37.0	37.0	37.0
8	36.447	37.0	37.0	37.0	37.0	37.0
9	36.5815	37.0	37.0	37.0	37.0	37.0
10	36.473	37.0	37.0	37.0	37.0	37.0
11	36.5495	37.0	37.0	37.0	37.0	37.0
12	36.506	37.0	37.0	37.0	37.0	37.0
13	36.55	37.0	37.0	37.0	37.0	37.0
14	36.486	37.0	37.0	37.0	37.0	37.0
15	36.539	37.0	37.0	37.0	37.0	37.0
16	36.5455	37.0	37.0	37.0	37.0	37.0
17	36.4175	37.0	37.0	37.0	37.0	37.0
18	36.4855	37.0	37.0	37.0	37.0	37.0
19	36.353	37.0	37.0	37.0	37.0	37.0
20	36.3245	37.0	37.0	37.0	37.0	37.0
21	36.4975	37.0	37.0	37.0	37.0	37.0
22	36.3685	37.0	37.0	37.0	37.0	37.0
23	36.463	37.0	37.0	37.0	37.0	37.0
24	36.3825	37.0	37.0	37.0	37.0	37.0
25	36.501	37.0	37.0	37.0	37.0	37.0
26	36.3665	37.0	37.0	37.0	37.0	37.0
27	36.4755	37.0	37.0	37.0	37.0	37.0
28	36.466	37.0	37.0	37.0	37.0	37.0
29	36.3615	37.0	37.0	37.0	37.0	37.0
30	36.467	37.0	37.0	37.0	37.0	37.0
31	36.512	37.0	37.0	37.0	37.0	37.0
32	36.367	37.0	37.0	37.0	37.0	37.0
33	36.5205	37.0	37.0	37.0	37.0	37.0
34	36.3085	37.0	37.0	37.0	37.0	37.0
35	36.261	37.0	37.0	37.0	37.0	37.0
36	36.3095	37.0	37.0	37.0	37.0	37.0
37	36.2605	37.0	37.0	37.0	37.0	37.0
38	36.449	37.0	37.0	37.0	37.0	37.0
39	36.3085	37.0	37.0	37.0	37.0	37.0
40	36.1855	37.0	37.0	37.0	37.0	37.0
41	36.258	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	1.0
23	0.0
24	1.0
25	2.0
26	3.0
27	5.0
28	12.0
29	18.0
30	25.0
31	27.0
32	42.0
33	62.0
34	103.0
35	233.0
36	1275.0
37	2190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.75	18.175	46.9	11.175
2	33.675	17.4	27.175	21.75
3	35.8	19.7	24.25	20.25
4	21.075	23.825	36.925000000000004	18.175
5	38.45	21.575	19.400000000000002	20.575
6	37.775	22.375	17.75	22.1
7	22.55	34.975	19.15	23.325000000000003
8	38.9	14.124999999999998	20.325	26.650000000000002
9	25.474999999999998	13.850000000000001	19.625	41.05
10	26.825	14.35	35.55	23.275000000000002
11	40.375	17.849999999999998	17.675	24.099999999999998
12	40.849999999999994	18.05	19.650000000000002	21.45
13	25.074999999999996	18.9	17.625	38.4
14	41.4	20.875	18.85	18.875
15	43.1	21.025	17.675	18.2
16	41.775	21.375	17.549999999999997	19.3
17	24.25	37.075	20.075000000000003	18.6
18	39.775	17.299999999999997	22.650000000000002	20.275000000000002
19	25.95	18.45	36.975	18.625
20	24.575	17.375	35.199999999999996	22.85
21	43.375	20.575	16.925	19.125
22	23.025000000000002	36.225	20.549999999999997	20.200000000000003
23	21.575	36.525	18.975	22.925
24	21.625	17.075000000000003	21.224999999999998	40.075
25	28.375	15.174999999999999	19.175	37.275000000000006
26	24.05	18.325	21.175	36.449999999999996
27	39.925	19.950000000000003	18.475	21.65
28	22.975	14.85	22.275	39.900000000000006
29	39.225	16.2	25.1	19.475
30	20.325	18.099999999999998	40.375	21.2
31	21.75	19.525000000000002	20.150000000000002	38.574999999999996
32	21.525	21.95	19.425	37.1
33	39.6	22.325	19.15	18.925
34	19.3	40.849999999999994	19.825	20.025000000000002
35	20.375	38.475	20.25	20.9
36	21.375	38.25	20.95	19.425
37	22.650000000000002	20.625	35.375	21.349999999999998
38	21.625	22.25	34.8	21.325
39	20.9	20.875	18.5	39.725
40	20.1	21.025	18.575	40.300000000000004
41	20.474999999999998	20.349999999999998	38.224999999999994	20.95
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	1.0
20	1.0
21	0.5
22	0.0
23	0.0
24	0.0
25	0.0
26	1.0
27	2.0
28	7.0
29	12.0
30	9.0
31	6.0
32	6.0
33	12.5
34	19.0
35	26.0
36	33.0
37	33.0
38	50.0
39	67.0
40	99.5
41	132.0
42	132.0
43	164.5
44	197.0
45	280.5
46	364.0
47	364.0
48	351.0
49	338.0
50	380.5
51	423.0
52	429.5
53	436.0
54	436.0
55	447.0
56	458.0
57	371.5
58	285.0
59	285.0
60	646.5
61	1008.0
62	565.5
63	123.0
64	123.0
65	84.0
66	45.0
67	36.0
68	27.0
69	27.0
70	19.5
71	12.0
72	9.5
73	7.0
74	4.0
75	1.0
76	1.0
77	1.5
78	2.0
79	1.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.5
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.47500000000001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.36874702239162	39.025
2	16.72224868985231	17.549999999999997
3	4.335397808480228	6.825
4	1.6198189614101954	3.4000000000000004
5	0.857551214864221	2.25
6	0.6193425440686041	1.95
7	0.5717008099094807	2.1
8	0.23820867079561697	1.0
9	0.09528346831824679	0.44999999999999996
>10	0.38113387327298714	3.5000000000000004
>50	0.14292520247737017	4.8
>100	0.0	0.0
>500	0.04764173415912339	17.150000000000002
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCT	686	17.150000000000002	No Hit
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCT	82	2.0500000000000003	TruSeq Adapter, Index 8 (97% over 37bp)
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTAC	55	1.375	No Hit
GGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTA	55	1.375	No Hit
GCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACA	31	0.775	No Hit
GGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAACC	26	0.65	No Hit
GGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACAT	19	0.475	No Hit
AAAAAACGCGCCCGAAGGCGCGTTGGCGATACACTCAATAT	17	0.42500000000000004	No Hit
GGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAAC	16	0.4	No Hit
AGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAA	11	0.27499999999999997	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCC	10	0.25	No Hit
TGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	10	0.25	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACGAGAGGACCGGGA	9	0.22499999999999998	No Hit
TGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTC	9	0.22499999999999998	No Hit
TATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAG	8	0.2	No Hit
CGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACAG	8	0.2	No Hit
GCAGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCA	8	0.2	No Hit
GTTTTAACGCTTCAACCCCAGGCAGGGCTTCCACGCGATCT	8	0.2	No Hit
ACAAACGGCACGACACAGCTGTCGTACCGTTATTGCTTATT	8	0.2	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAAC	7	0.17500000000000002	No Hit
GCAGGGCTTCCACGCGATCTCTTTTGGGTTTGACCTCTCTT	7	0.17500000000000002	No Hit
GCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACTAC	7	0.17500000000000002	No Hit
ACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACTGGG	7	0.17500000000000002	No Hit
AAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAA	7	0.17500000000000002	No Hit
TCTGATCCACGGTGAATACGTTCCCGGGCCTTGTACACACC	7	0.17500000000000002	No Hit
GTCGCCCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAAT	7	0.17500000000000002	No Hit
AGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGT	7	0.17500000000000002	No Hit
TCTCGCGAGGTCGGAATCGCTAGTAATCGTGGATCAGAATG	7	0.17500000000000002	No Hit
TCAACCCCAGGCAGGGCTTCCACGCGATCTCTTTTGGGTTT	7	0.17500000000000002	No Hit
TTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCAC	7	0.17500000000000002	No Hit
TAGCTACCGAGATGAGTTCTCCCTGACTCCTTGAGAGTCCT	7	0.17500000000000002	No Hit
GACACGCCACTAACAAACTAGCCTGATTAAGTTTTAACGCT	6	0.15	No Hit
TCCACGCGATCTCTTTTGGGTTTGACCTCTCTTGATCCCCG	6	0.15	No Hit
GGTTAAGCTACCTACTTTGTGATTCATGACTGGGGTGAAGT	6	0.15	No Hit
GATCCCCGTCCTAAGAGCGGAGGCTAGGGAGAGAGGGCTCT	6	0.15	No Hit
ACTAGCGATTCCCGGGCCTTGTACACACCGCCCGTCACACC	6	0.15	No Hit
GTTTGACCTCTCTTGATCCCCGTCCTAAGAGCGGAGGCTAG	6	0.15	No Hit
TTTATGAGGTCGTAGTCCGGATTGGAGTCTGCAACTCGACT	6	0.15	No Hit
GTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTC	6	0.15	No Hit
TTCCTACATCCTCGGTACTACATGCTTAGTCAGTCTTTACA	6	0.15	No Hit
GATCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCC	6	0.15	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCC	6	0.15	No Hit
GTCTTCAACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATG	6	0.15	No Hit
GAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCG	6	0.15	No Hit
TTCCGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTT	5	0.125	No Hit
AAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGC	5	0.125	No Hit
TTTGACCTCTCTTGATCCCCGTCCTAAGAGCGGAGGCTAGG	5	0.125	No Hit
TTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACT	5	0.125	No Hit
TTGACGTCATCATGGCCCTTACGACCAGGGCTACACACGTG	5	0.125	No Hit
ATGCCTGGCAGTTCCCCCCCAGATCGGAAGAGCACACGTCT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAGTCTTTACATTCGCTTGCCAGCTGCGGACGGACACGCC	5	0.125	No Hit
TCTCTTTTGGGTTTGACCTCTCTTGATCCCCGTCCTAAGAG	5	0.125	No Hit
AAAAACGCGCCCGAAGGCGCGTTGGCGATACACTCAATATA	5	0.125	No Hit
TTTATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGA	5	0.125	No Hit
TTCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	5	0.125	No Hit
TCATGGAGTCGAGTTGCTAGTAATCGTGGATCAGAATGCCA	5	0.125	No Hit
TCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACA	5	0.125	No Hit
ATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCA	5	0.125	No Hit
ATAAGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG	5	0.125	No Hit
GATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGC	5	0.125	No Hit
TTTTAACGCTTCAACCCCAGGCAGGGCTTCCACGCGATCTC	5	0.125	No Hit
CTTCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	2.05	0.0	0.0	0.0	0.0
5	2.075	0.0	0.0	0.0	0.0
6	2.075	0.0	0.0	0.0	0.0
7	2.075	0.0	0.0	0.0	0.0
8	2.075	0.0	0.0	0.0	0.0
9	2.1	0.0	0.0	0.0	0.0
10	2.1	0.0	0.0	0.0	0.0
11	2.1	0.0	0.0	0.0	0.0
12	2.1	0.0	0.0	0.0	0.0
13	2.125	0.0	0.0	0.0	0.0
14	2.125	0.0	0.0	0.0	0.0
15	2.125	0.0	0.0	0.0	0.0
16	2.15	0.0	0.0	0.0	0.0
17	2.15	0.0	0.0	0.0	0.0
18	2.15	0.0	0.0	0.0	0.0
19	2.175	0.0	0.0	0.0	0.0
20	2.225	0.0	0.0	0.0	0.0
21	2.35	0.0	0.0	0.0	0.0
22	2.425	0.0	0.0	0.0	0.0
23	2.425	0.0	0.0	0.0	0.0
24	2.45	0.0	0.0	0.0	0.0
25	2.475	0.0	0.0	0.0	0.0
26	2.525	0.0	0.0	0.0	0.0
27	2.625	0.0	0.0	0.0	0.0
28	2.65	0.0	0.0	0.0	0.0
29	2.7	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTGGA	75	0.0	32.666668	1
CACACGT	25	0.0063971295	27.999998	16
GTGGAGC	90	0.0	27.222223	3
GGTGGAG	95	0.0	25.789474	2
TGGAGCT	100	0.0	24.499998	4
GCGGGAG	105	1.8189894E-12	23.333334	12
GTTGAAC	105	1.8189894E-12	23.333334	18
AATTCCT	105	1.8189894E-12	23.333334	35
GTCCGAA	105	1.8189894E-12	23.333334	29
AACCCGC	105	1.8189894E-12	23.333334	22
ACCCGCG	105	1.8189894E-12	23.333334	23
GGGAGTT	105	1.8189894E-12	23.333334	14
GAGTTGA	105	1.8189894E-12	23.333334	16
CCCGCGT	105	1.8189894E-12	23.333334	24
GAACCCG	105	1.8189894E-12	23.333334	21
CGGGAGT	105	1.8189894E-12	23.333334	13
GCGTCCG	105	1.8189894E-12	23.333334	27
CGAAATT	105	1.8189894E-12	23.333334	32
AGTTGAA	105	1.8189894E-12	23.333334	17
TTGAACC	105	1.8189894E-12	23.333334	19
>>END_MODULE
SRR23332654 read2 length is 38-50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332654_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	38-50
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.437	11.0	11.0	37.0	11.0	37.0
2	22.33925	25.0	11.0	37.0	11.0	37.0
3	25.0495	25.0	11.0	37.0	11.0	37.0
4	35.864	37.0	37.0	37.0	37.0	37.0
5	35.998	37.0	37.0	37.0	37.0	37.0
6	35.9935	37.0	37.0	37.0	37.0	37.0
7	35.726	37.0	37.0	37.0	37.0	37.0
8	35.96	37.0	37.0	37.0	37.0	37.0
9	35.7	37.0	37.0	37.0	37.0	37.0
10	35.7835	37.0	37.0	37.0	37.0	37.0
11	35.8475	37.0	37.0	37.0	37.0	37.0
12	35.7925	37.0	37.0	37.0	37.0	37.0
13	35.8785	37.0	37.0	37.0	37.0	37.0
14	35.838	37.0	37.0	37.0	37.0	37.0
15	35.847	37.0	37.0	37.0	37.0	37.0
16	35.962	37.0	37.0	37.0	37.0	37.0
17	35.99925	37.0	37.0	37.0	37.0	37.0
18	36.0025	37.0	37.0	37.0	37.0	37.0
19	35.836	37.0	37.0	37.0	37.0	37.0
20	36.0015	37.0	37.0	37.0	37.0	37.0
21	35.83275	37.0	37.0	37.0	37.0	37.0
22	35.9875	37.0	37.0	37.0	37.0	37.0
23	36.12	37.0	37.0	37.0	37.0	37.0
24	36.085	37.0	37.0	37.0	37.0	37.0
25	35.85875	37.0	37.0	37.0	37.0	37.0
26	36.0155	37.0	37.0	37.0	37.0	37.0
27	35.79975	37.0	37.0	37.0	37.0	37.0
28	36.01475	37.0	37.0	37.0	37.0	37.0
29	35.97	37.0	37.0	37.0	37.0	37.0
30	35.9065	37.0	37.0	37.0	37.0	37.0
31	35.954	37.0	37.0	37.0	37.0	37.0
32	35.902	37.0	37.0	37.0	37.0	37.0
33	35.899	37.0	37.0	37.0	37.0	37.0
34	35.97975	37.0	37.0	37.0	37.0	37.0
35	36.059	37.0	37.0	37.0	37.0	37.0
36	35.944	37.0	37.0	37.0	37.0	37.0
37	35.9685	37.0	37.0	37.0	37.0	37.0
38	35.8735	37.0	37.0	37.0	37.0	37.0
39	35.96174043510878	37.0	37.0	37.0	37.0	37.0
40	35.92171085542771	37.0	37.0	37.0	37.0	37.0
41	35.709709709709706	37.0	37.0	37.0	37.0	37.0
42	35.726226226226224	37.0	37.0	37.0	37.0	37.0
43	35.594689378757515	37.0	37.0	37.0	37.0	37.0
44	35.877024291497975	37.0	37.0	37.0	37.0	37.0
45	35.62952281069743	37.0	37.0	37.0	37.0	37.0
46	35.729486500794074	37.0	37.0	37.0	37.0	37.0
47	35.92782398712101	37.0	37.0	37.0	37.0	37.0
48	35.93009284543965	37.0	37.0	37.0	37.0	37.0
49	35.88706739526412	37.0	37.0	37.0	37.0	37.0
50	35.73916184971098	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	0.0
16	0.0
17	1.0
18	1.0
19	4.0
20	2.0
21	8.0
22	12.0
23	12.0
24	10.0
25	15.0
26	20.0
27	18.0
28	24.0
29	33.0
30	39.0
31	62.0
32	92.0
33	156.0
34	482.0
35	1749.0
36	1089.0
37	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	40.9	23.150000000000002	35.949999999999996
2	34.28357089272318	34.40860215053764	11.727931982995749	19.579894973743436
3	29.849999999999998	38.525	13.125	18.5
4	82.175	7.8	4.175	5.8500000000000005
5	69.525	14.399999999999999	8.725	7.35
6	61.775000000000006	18.4	9.275	10.549999999999999
7	41.0	38.074999999999996	8.924999999999999	12.0
8	42.875	18.35	12.825000000000001	25.95
9	20.4	38.550000000000004	30.25	10.8
10	45.050000000000004	21.95	16.45	16.55
11	27.925	42.3	15.525	14.249999999999998
12	21.6	26.125	29.525000000000002	22.75
13	36.199999999999996	13.900000000000002	32.25	17.65
14	34.55	14.274999999999999	23.400000000000002	27.775
15	26.6	18.5	45.15	9.75
16	49.7	19.225	20.925	10.15
17	29.457364341085274	32.05801450362591	17.479369842460617	21.005251312828207
18	15.8	40.575	28.199999999999996	15.425
19	20.1	16.675	37.425000000000004	25.8
20	14.75	21.6	35.275	28.375
21	19.579894973743436	40.910227556889225	11.202800700175043	28.307076769192296
22	42.575	28.925	11.975	16.525000000000002
23	21.775	33.2	18.125	26.900000000000002
24	23.5	19.650000000000002	13.075000000000001	43.775
25	43.51087771942986	14.603650912728183	11.40285071267817	30.48262065516379
26	37.275000000000006	19.3	11.675	31.75
27	41.61040260065016	25.406351587896975	18.779694923730933	14.20355088772193
28	31.857964491122782	27.981995498874717	25.531382845711427	14.628657164291074
29	24.7	13.275	49.05	12.975
30	18.234117058529264	14.732366183091546	54.70235117558779	12.331165582791396
31	19.025	19.45	42.275	19.25
32	36.418209104552275	11.78089044522261	19.609804902451224	32.191095547773884
33	21.775	13.65	13.375	51.2
34	30.723042281711283	16.86264698523893	20.665499124343256	31.74881160870653
35	12.875	38.65	20.225	28.249999999999996
36	12.506253126563283	43.1215607803902	27.688844422211105	16.683341670835418
37	15.2	48.475	11.75	24.575
38	20.575	18.6	10.475	50.349999999999994
39	34.93373343335834	16.579144786196547	11.127781945486372	37.35933983495874
40	24.668501376032022	18.88916687515637	17.538153615211407	38.9041781336002
41	17.117117117117118	42.592592592592595	26.426426426426424	13.863863863863864
42	20.62062062062062	30.83083083083083	28.72872872872873	19.81981981981982
43	35.120240480961925	22.44488977955912	15.931863727454909	26.50300601202405
44	41.32085020242915	13.461538461538462	19.02834008097166	26.18927125506073
45	17.83372672436402	14.293207448203516	37.76553894571204	30.107526881720432
46	33.615669666490206	18.025410269984118	30.99523557437798	17.363684489147698
47	28.575261604507645	14.944995975315267	26.401931848671857	30.077810571505232
48	27.44401966138722	27.74440196613872	25.341343528126707	19.47023484434735
49	28.827460510328066	10.753341433778859	45.6865127582017	14.732685297691372
50	37.35549132947977	12.861271676300579	26.11994219653179	23.66329479768786
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	0.5
24	0.0
25	1.5
26	3.5
27	3.5
28	3.5
29	9.5
30	16.0
31	18.0
32	22.5
33	34.0
34	42.5
35	43.0
36	44.0
37	54.5
38	64.5
39	60.5
40	57.0
41	75.0
42	90.5
43	84.5
44	87.0
45	100.0
46	104.0
47	129.0
48	162.5
49	177.5
50	211.5
51	385.0
52	532.0
53	689.0
54	873.5
55	877.0
56	821.0
57	675.5
58	517.5
59	350.5
60	211.5
61	137.0
62	76.5
63	65.0
64	52.5
65	42.0
66	31.0
67	20.0
68	11.5
69	8.0
70	6.5
71	5.0
72	4.0
73	3.0
74	1.5
75	0.5
76	0.0
77	0.5
78	1.0
79	1.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	1.5
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	2.0
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.025
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.025
18	0.0
19	0.0
20	0.0
21	0.025
22	0.0
23	0.0
24	0.0
25	0.025
26	0.0
27	0.025
28	0.025
29	0.0
30	0.05
31	0.0
32	0.05
33	0.0
34	0.075
35	0.0
36	0.05
37	0.0
38	0.0
39	0.0
40	0.02501250625312656
41	0.0
42	0.0
43	0.0
44	0.0
45	0.026219192448872573
46	0.0
47	0.0
48	0.0
49	0.060716454159077116
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
38	1.0
39	1.0
40	2.0
41	0.0
42	4.0
43	40.0
44	138.0
45	36.0
46	51.0
47	65.0
48	368.0
49	526.0
50	2768.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.900000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.19677996422182	47.625
2	5.500894454382826	6.15
3	2.0572450805008944	3.45
4	1.4311270125223614	3.2
5	0.7602862254025045	2.125
6	0.7602862254025045	2.55
7	0.8944543828264758	3.5000000000000004
8	0.4919499105545617	2.1999999999999997
9	0.35778175313059035	1.7999999999999998
>10	2.459749552772809	23.025000000000002
>50	0.044722719141323794	1.8499999999999999
>100	0.044722719141323794	2.5250000000000004
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	101	2.5250000000000004	No Hit
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	74	1.8499999999999999	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	50	1.25	No Hit
AAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	43	1.075	No Hit
CAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	37	0.9249999999999999	No Hit
CCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	32	0.8	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	29	0.7250000000000001	No Hit
CCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	28	0.7000000000000001	No Hit
ACTGGATCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGAG	27	0.675	Illumina Single End PCR Primer 1 (97% over 34bp)
CTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	23	0.575	No Hit
AACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	23	0.575	No Hit
TGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	22	0.5499999999999999	No Hit
CACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	21	0.525	No Hit
AAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	21	0.525	No Hit
CGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	21	0.525	No Hit
TAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	20	0.5	No Hit
CGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	19	0.475	No Hit
AATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	19	0.475	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	18	0.44999999999999996	No Hit
AGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	17	0.42500000000000004	No Hit
ACAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	17	0.42500000000000004	No Hit
CAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	16	0.4	No Hit
AGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
AGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	16	0.4	No Hit
CGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
CGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
AGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
CGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
TGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
TGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	14	0.35000000000000003	No Hit
TGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	14	0.35000000000000003	No Hit
AAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
ATAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
CCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
CGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
CGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
CAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	12	0.3	No Hit
TATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
CCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
AAAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	11	0.27499999999999997	No Hit
TCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
AAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
ACAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
CCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
CCCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
TGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
ACCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
AGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
ACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
TGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
CGCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
CAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
CTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
CCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	9	0.22499999999999998	No Hit
CCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
CAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
AACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
TGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
AAAGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTA	8	0.2	No Hit
AACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
CAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
AGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
CTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
AGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
AAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CCCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
CTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
ATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
AATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
ACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CCGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
TAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
AATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TTTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TGTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TTGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CTAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
AGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
ATAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
AAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
ATCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TGCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TGCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
ACAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
AAAACTGGATCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGAG	6	0.15	Illumina Single End PCR Primer 1 (97% over 34bp)
TAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
ACAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
ATAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
AGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TTGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
ATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
ACCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
ACCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
ACTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CGTACTGGATCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGAG	5	0.125	Illumina Single End PCR Primer 1 (97% over 34bp)
CCTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
ATAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.05	0.0	0.0	0.0	0.0
10	0.775	0.0	0.0	0.0	0.0
11	0.975	0.0	0.0	0.0	0.0
12	1.125	0.0	0.0	0.0	0.0
13	2.175	0.0	0.0	0.0	0.0
14	2.2	0.0	0.0	0.0	0.0
15	2.2	0.0	0.0	0.0	0.0
16	2.2	0.0	0.0	0.0	0.0
17	2.2	0.0	0.0	0.0	0.0
18	2.2	0.0	0.0	0.0	0.0
19	2.2	0.0	0.0	0.0	0.0
20	2.2	0.0	0.0	0.0	0.0
21	2.2	0.0	0.0	0.0	0.0
22	2.25	0.0	0.0	0.0	0.0
23	2.275	0.0	0.0	0.0	0.0
24	2.275	0.0	0.0	0.0	0.0
25	2.3	0.0	0.0	0.0	0.0
26	2.3	0.0	0.0	0.0	0.0
27	2.325	0.0	0.0	0.0	0.0
28	2.425	0.0	0.0	0.0	0.0
29	2.475	0.0	0.0	0.0	0.0
30	2.575	0.0	0.0	0.0	0.0
31	2.625	0.0	0.0	0.0	0.0
32	2.625	0.0	0.0	0.0	0.0
33	2.675	0.0	0.0	0.0	0.0
34	2.7	0.0	0.0	0.0	0.0
35	2.75	0.0	0.0	0.0	0.0
36	2.8	0.0	0.0	0.0	0.0
37	2.875	0.0	0.0	0.0	0.0
38	2.925	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCATG	110	0.0	52.590908	44
GGTGCAT	110	0.0	43.44466	43
CGGGGCT	35	1.14214345E-7	43.3875	3
TGGGGCT	20	7.4493483E-4	43.3875	3
AGGTGCA	110	0.0	39.44318	42
AAGGTGC	110	0.0	39.44318	41
GGCTGAT	105	0.0	39.25536	6
GCTGATT	105	0.0	39.25536	7
GGGGCTG	105	0.0	39.25536	4
GGGCTGA	105	0.0	39.25536	5
CAAGGTG	110	0.0	38.93093	40
CCAAGGT	110	0.0	38.93093	39
CCCAAGG	105	0.0	37.660038	38
TCTGGAT	110	0.0	37.47102	13
TGATTCT	110	0.0	37.47102	9
GACGGGA	110	0.0	37.47102	22
GGATTTG	110	0.0	37.47102	26
TTCTGGA	110	0.0	37.47102	12
TGCGAAA	110	0.0	37.47102	31
CTGATTC	110	0.0	37.47102	8
>>END_MODULE
Read 17761 spots for SRR23332654.sra
Written 17761 spots for SRR23332654.sra
Read 17763 spots for SRR23332654.sra
Written 17763 spots for SRR23332654.sra
Read 17761 spots for SRR23332654.sra
Written 17761 spots for SRR23332654.sra
Read 17761 spots for SRR23332654.sra
Written 17761 spots for SRR23332654.sra
Read 17761 spots for SRR23332654.sra
Written 17761 spots for SRR23332654.sra
Read 17761 spots for SRR23332654.sra
Written 17761 spots for SRR23332654.sra
Read 17761 spots for SRR23332654.sra
Written 17761 spots for SRR23332654.sra
Read 17761 spots for SRR23332654.sra
Written 17761 spots for SRR23332654.sra
SRR ids: ['SRR23332654.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_gdvra5wj
SRR23332654.sra spots: 142090
blocks: [[1, 17761], [17762, 35522], [35523, 53283], [53284, 71044], [71045, 88805], [88806, 106566], [106567, 124327], [124328, 142090]]
SRR23332654 file size 16852
SRR23332654 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR23332654 SRR23332654_1.fastq SRR23332654_2.fastq
Input file:	SRR23332654_1.fastq
Paired file:	SRR23332654_2.fastq
trimmed:	SRR23332654-trimmed-pair1.fastq, SRR23332654-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 05:09:09 2026 >> started

Wed Mar 25 05:09:09 2026 >> done (0.182s)
142090 read pairs processed; of these:
    28 ( 0.02%) short read pairs filtered out after trimming by size control
     0 ( 0.00%) empty read pairs filtered out after trimming by size control
142062 (99.98%) read pairs available; of these:
   930 ( 0.65%) trimmed read pairs available after processing
141132 (99.35%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     7	  0.00%
 19	     7	  0.00%
 20	    19	  0.01%
 21	     7	  0.00%
 22	     7	  0.00%
 23	    12	  0.01%
 24	     9	  0.01%
 25	    27	  0.02%
 26	    20	  0.01%
 27	    20	  0.01%
 28	    14	  0.01%
 29	    76	  0.05%
 30	    18	  0.01%
 31	    23	  0.02%
 32	    33	  0.02%
 33	    36	  0.03%
 34	    47	  0.03%
 35	    61	  0.04%
 36	    41	  0.03%
 37	    48	  0.03%
 38	    68	  0.05%
 39	    69	  0.05%
 40	   162	  0.11%
 41	   162	  0.11%
 42	  6128	  4.31%
 43	  2794	  1.97%
 44	 15194	 10.70%
 45	116953	 82.33%
142062 reads passed initial QC


criterion=sequence-density
sequence-density=16.79
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=29
prefix-density=0.00
prefix-fanout=1.0
sequence=TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACATCCTCGGTACTACATGCTTAGTCAGTCTTTACATTCGCTTGCCAGCTGCGGACGGACACGCCACTAACAAACTAGCCTGATTAAGTTTTAACGCTTCAACCCCAGGCAGGGCTTCCACGCGATCTCTTTTGGGTTTGACCTCTCTTGATCCCCGTCCTAAGAGCGGAGGCTAGGGAGAGAGGGCTCTAAGCAGGTTATTAAGCTGCTAAAGCGTAGTTTTCGTCGTTTGCGACTATTTTTTGCGGCTTTTTACGAGGCCAACCGCCCCTCGGCATGCACCTT


criterion=fanout-score
sequence-density=0.13
sequence-density-rank=30
fanout-score=5.38
fanout-score-rank=1
prefix-density=0.37
prefix-fanout=1.8
sequence=TCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGAGTTCT


criterion=sequence-density
sequence-density=19.35
sequence-density-rank=1
fanout-score=19.25
fanout-score-rank=3
prefix-density=15.80
prefix-fanout=19.3
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTA


criterion=fanout-score
sequence-density=0.47
sequence-density-rank=11
fanout-score=203.86
fanout-score-rank=1
prefix-density=9.12
prefix-fanout=10.6
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTACTGGATCG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACATCCTCGGTACTACATGCTTAGTCAGTCTTTACATTCGCTTGCCAGCTGCGGACGGACACGCCACTAACAAACTAGCCTGATTAAGTTTTAACGCTTCAACCCCAGGCAGGGCTTCCACGCGATCTCTTTTGGGTTTGACCTCTCTTGATCCCCGTCCTAAGAGCGGAGGCTAGGGAGAGAGGGCTCTAAGCAGGTTATTAAGCTGCTAAAGCGTAGTTTTCGTCGTTTGCGACTATTTTTTGCGGCTTTTTACGAGGCCAACCGCCCCTCGGCATGCACCTT -y GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTA -o SRR23332654 SRR23332654_1.fastq SRR23332654_2.fastq
Input file:	SRR23332654_1.fastq
Paired file:	SRR23332654_2.fastq
trimmed:	SRR23332654-trimmed-pair1.fastq, SRR23332654-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACATCCTCGGTACTACATGCTTA
-- paired 3' end adapter sequence (-y):	GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 05:09:10 2026 >> started

Wed Mar 25 05:09:10 2026 >> done (0.161s)
127108 read pairs processed; of these:
   409 ( 0.32%) short read pairs filtered out after trimming by size control
 24910 (19.60%) empty read pairs filtered out after trimming by size control
101789 (80.08%) read pairs available; of these:
    58 ( 0.06%) trimmed read pairs available after processing
101731 (99.94%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     5	  0.00%
 19	     7	  0.01%
 20	    17	  0.02%
 21	     4	  0.00%
 22	     5	  0.00%
 23	    10	  0.01%
 24	     7	  0.01%
 25	    24	  0.02%
 26	    17	  0.02%
 27	    18	  0.02%
 28	    10	  0.01%
 29	    71	  0.07%
 30	    17	  0.02%
 31	    21	  0.02%
 32	    30	  0.03%
 33	    33	  0.03%
 34	    41	  0.04%
 35	    55	  0.05%
 36	    38	  0.04%
 37	    39	  0.04%
 38	    60	  0.06%
 39	    59	  0.06%
 40	   149	  0.15%
 41	   112	  0.11%
 42	  5198	  5.11%
 43	  2308	  2.27%
 44	 12064	 11.85%
 45	 81370	 79.94%


criterion=sequence-density
sequence-density=2.28
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=29
prefix-density=0.00
prefix-fanout=1.0
sequence=TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACATCCTCGGTACTACATGCTTAGTCAGTCTTTACATTCGCTTGCCAGCTGCGGACGGACACGCCACTAACAAACTAGCCTGATTAAGTTTTAACGCTTCAACCCCAGGCAGGGCTTCCACGCGATCTCTTTTGGGTTTGACCTCTCTTGATCCCCGTCCTAAGAGCGGAGGCTAGGGAGAGAGGGCTCTAAGCAGGTTATTAAGCTGCTAAAGCGTAGTTTTCGTCGTTTGCGACTATTTTTTGCGGCTTTTTACGAGGCCAACCGCCCCTCGGCATGCACCT


criterion=fanout-score
sequence-density=0.16
sequence-density-rank=30
fanout-score=5.38
fanout-score-rank=1
prefix-density=0.46
prefix-fanout=1.8
sequence=TCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGAGTTCT


criterion=sequence-density
sequence-density=18.14
sequence-density-rank=1
fanout-score=21.30
fanout-score-rank=2
prefix-density=13.24
prefix-fanout=21.3
sequence=GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTA


criterion=fanout-score
sequence-density=0.58
sequence-density-rank=10
fanout-score=203.86
fanout-score-rank=1
prefix-density=11.09
prefix-fanout=10.6
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTACTGGATCG
SRR23332654 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 25 05:09:25
                             Started mapping on |	Mar 25 05:09:25
                                    Finished on |	Mar 25 05:09:55
       Mapping speed, Million of reads per hour |	14.01

                          Number of input reads |	116743
                      Average input read length |	90
                                    UNIQUE READS:
                   Uniquely mapped reads number |	52175
                        Uniquely mapped reads % |	44.69%
                          Average mapped length |	87.60
                       Number of splices: Total |	4734
            Number of splices: Annotated (sjdb) |	0
                       Number of splices: GT/AG |	4360
                       Number of splices: GC/AG |	85
                       Number of splices: AT/AC |	18
               Number of splices: Non-canonical |	271
                      Mismatch rate per base, % |	0.33%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.45
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	30118
             % of reads mapped to multiple loci |	25.80%
        Number of reads mapped to too many loci |	23490
             % of reads mapped to too many loci |	20.12%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	8.96%
                     % of reads unmapped: other |	0.42%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	34450	34450	34450
N_multimapping	30118	30118	30118
N_noFeature	2486	44576	5176
N_ambiguous	7422	1134	1769
UnstrandedReadsAssigned:42267 PositiveStrandReadsAssigned:6465 NegativeStrandReadsAssigned:45230
Dataset is classified negative stranded
MeadianReadLen=41 20thPercentileLength=41 echo kmer=37
SRR23332654 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR23332654-trimmed-pair1.fastq
                             SRR23332654-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 116,743 reads, 21,983 reads pseudoaligned
[quant] estimated average fragment length: 201.291
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 52 rounds

  4323 SRR23332654.ke.tsv
  4497 SRR23332654.se.tsv
  8820 total
==> SRR23332654.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	16.375	7	2592.32
AAC73113	2463	2262.71	4	10.7202
AAC73114	933	732.709	0	0
AAC73115	1287	1086.71	1	5.58031
AAC73116	297	122.479	0	0
AAC73117	777	576.709	0	0
AAC73118	1431	1230.71	1	4.92738
AAC73119	954	753.709	3	24.1373
AAC73120	588	389.447	1	15.5712

==> SRR23332654.se.tsv <==
b0001	15
b0002	4
b0003	0
b0004	1
b0005	0
b0006	0
b0007	1
b0008	3
b0009	1
b0010	0
SRR23332654 completed mapping pipeline successfully
