Starting /dee2/code/volunteer_pipeline.sh SRR23332681
    current disk space = 1464424152
    free memory = 58880948 
SRR23332681 SRAfilesize
14bb0dc60660716ca31b63ae60c7f385  SRR23332681.sra
SRR23332681.sra file validated
SRR23332681 is paired end
SRR23332681 is conventional basespace
SRR23332681 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332681_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.439	37.0	37.0	37.0	37.0	37.0
2	36.4415	37.0	37.0	37.0	37.0	37.0
3	36.4995	37.0	37.0	37.0	37.0	37.0
4	36.4515	37.0	37.0	37.0	37.0	37.0
5	36.3895	37.0	37.0	37.0	37.0	37.0
6	36.389	37.0	37.0	37.0	37.0	37.0
7	36.436	37.0	37.0	37.0	37.0	37.0
8	36.4315	37.0	37.0	37.0	37.0	37.0
9	36.5375	37.0	37.0	37.0	37.0	37.0
10	36.519	37.0	37.0	37.0	37.0	37.0
11	36.466	37.0	37.0	37.0	37.0	37.0
12	36.4825	37.0	37.0	37.0	37.0	37.0
13	36.5365	37.0	37.0	37.0	37.0	37.0
14	36.4415	37.0	37.0	37.0	37.0	37.0
15	36.537	37.0	37.0	37.0	37.0	37.0
16	36.482	37.0	37.0	37.0	37.0	37.0
17	36.4355	37.0	37.0	37.0	37.0	37.0
18	36.42	37.0	37.0	37.0	37.0	37.0
19	36.3945	37.0	37.0	37.0	37.0	37.0
20	36.4775	37.0	37.0	37.0	37.0	37.0
21	36.5705	37.0	37.0	37.0	37.0	37.0
22	36.3705	37.0	37.0	37.0	37.0	37.0
23	36.4475	37.0	37.0	37.0	37.0	37.0
24	36.392	37.0	37.0	37.0	37.0	37.0
25	36.466	37.0	37.0	37.0	37.0	37.0
26	36.3905	37.0	37.0	37.0	37.0	37.0
27	36.5005	37.0	37.0	37.0	37.0	37.0
28	36.519	37.0	37.0	37.0	37.0	37.0
29	36.4575	37.0	37.0	37.0	37.0	37.0
30	36.4075	37.0	37.0	37.0	37.0	37.0
31	36.467	37.0	37.0	37.0	37.0	37.0
32	36.5235	37.0	37.0	37.0	37.0	37.0
33	36.499	37.0	37.0	37.0	37.0	37.0
34	36.4265	37.0	37.0	37.0	37.0	37.0
35	36.353	37.0	37.0	37.0	37.0	37.0
36	36.236	37.0	37.0	37.0	37.0	37.0
37	36.3525	37.0	37.0	37.0	37.0	37.0
38	36.366	37.0	37.0	37.0	37.0	37.0
39	36.3365	37.0	37.0	37.0	37.0	37.0
40	36.278	37.0	37.0	37.0	37.0	37.0
41	36.2195	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	1.0
26	7.0
27	5.0
28	11.0
29	14.0
30	18.0
31	32.0
32	40.0
33	78.0
34	115.0
35	213.0
36	1264.0
37	2201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.15	20.3	46.525	9.025
2	29.599999999999998	18.2	30.3	21.9
3	33.775	20.674999999999997	25.4	20.150000000000002
4	22.125	25.275	33.800000000000004	18.8
5	33.575	26.224999999999998	20.549999999999997	19.650000000000002
6	36.075	23.7	19.875	20.349999999999998
7	21.475	34.425	20.325	23.775
8	38.324999999999996	15.875	21.025	24.775
9	26.625	14.625	20.525	38.224999999999994
10	26.650000000000002	17.150000000000002	31.874999999999996	24.325
11	36.65	17.474999999999998	18.675	27.200000000000003
12	38.875	18.0	21.25	21.875
13	26.400000000000002	19.475	17.349999999999998	36.775000000000006
14	39.225	21.25	19.375	20.150000000000002
15	37.875	23.200000000000003	18.375	20.549999999999997
16	40.550000000000004	20.75	18.95	19.75
17	25.650000000000002	34.625	22.825	16.900000000000002
18	36.5	19.1	24.575	19.825
19	24.725	20.925	35.225	19.125
20	24.65	19.6	34.449999999999996	21.3
21	38.625	24.175	17.9	19.3
22	24.575	32.15	21.55	21.725
23	22.675	31.974999999999998	19.400000000000002	25.95
24	24.0	18.425	19.6	37.974999999999994
25	27.800000000000004	16.650000000000002	20.925	34.625
26	23.075000000000003	18.45	23.25	35.225
27	37.75	19.325	20.175	22.75
28	20.674999999999997	16.1	24.65	38.574999999999996
29	36.35	16.125	25.1	22.425
30	21.45	16.625	38.85	23.075000000000003
31	22.625	18.375	24.2	34.8
32	22.075	23.075000000000003	21.85	33.0
33	33.85	24.95	22.6	18.6
34	20.875	38.725	20.525	19.875
35	21.875	35.625	22.650000000000002	19.85
36	20.95	35.225	21.0	22.825
37	19.85	20.175	35.35	24.625
38	21.875	20.75	33.550000000000004	23.825
39	20.05	21.15	21.925	36.875
40	20.0	25.0	20.1	34.9
41	18.425	21.175	36.375	24.025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	4.0
24	7.0
25	7.0
26	4.5
27	2.0
28	20.0
29	38.0
30	36.0
31	34.0
32	34.0
33	34.0
34	34.0
35	39.5
36	45.0
37	45.0
38	77.0
39	109.0
40	128.5
41	148.0
42	148.0
43	183.5
44	219.0
45	294.5
46	370.0
47	370.0
48	378.5
49	387.0
50	395.0
51	403.0
52	432.5
53	462.0
54	462.0
55	433.0
56	404.0
57	352.5
58	301.0
59	301.0
60	582.5
61	864.0
62	476.0
63	88.0
64	88.0
65	67.5
66	47.0
67	34.5
68	22.0
69	22.0
70	16.5
71	11.0
72	6.0
73	1.0
74	0.5
75	0.0
76	0.0
77	1.0
78	2.0
79	1.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.5
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.78270509977827	43.85
2	14.67849223946785	16.55
3	3.8580931263858096	6.525
4	1.3747228381374723	3.1
5	0.5764966740576497	1.625
6	0.31042128603104213	1.05
7	0.3991130820399113	1.575
8	0.13303769401330376	0.6
9	0.17738359201773835	0.8999999999999999
>10	0.6208425720620843	7.675
>50	0.0	0.0
>100	0.044345898004434586	2.65
>500	0.044345898004434586	13.900000000000002
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCT	556	13.900000000000002	No Hit
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTAC	106	2.65	No Hit
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAA	49	1.225	TruSeq Adapter, Index 6 (97% over 37bp)
GGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTA	49	1.225	No Hit
GCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACA	36	0.8999999999999999	No Hit
ACAAACGGCACGACACAGCTGTCGTACCGTTATTGCTTATT	27	0.675	No Hit
GGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACAT	25	0.625	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCC	20	0.5	No Hit
TGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACA	17	0.42500000000000004	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCC	16	0.4	No Hit
TGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	14	0.35000000000000003	No Hit
AAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTT	12	0.3	No Hit
AAAAAACGCGCCCGAAGGCGCGTTGGCGATACACTCAATAT	11	0.27499999999999997	No Hit
TTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCAC	11	0.27499999999999997	No Hit
GCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACTAC	10	0.25	No Hit
AAAAAAATGGCGCACAATGTGCGCCATTTTTCACTTCACAG	10	0.25	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACGAGAGGACCGGGA	9	0.22499999999999998	No Hit
TTTATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATAT	9	0.22499999999999998	No Hit
TCTCGTACTAGGAGTGGACGCATCACTGGTGTTCGGGTTGT	9	0.22499999999999998	No Hit
GTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTT	9	0.22499999999999998	No Hit
TTTTGCGTACTGTCAGAGCACTGATTTCTCTGACAGTACGT	8	0.2	No Hit
ACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTAC	8	0.2	No Hit
AGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCCA	8	0.2	No Hit
TAACAAACTAGCCTGATTAAGTTTTAACGCTTCAACCCCAG	7	0.17500000000000002	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAAC	7	0.17500000000000002	No Hit
GCAGGGCTTCCACGCGATCTCTTTTGGGTTTGACCTCTCTT	7	0.17500000000000002	No Hit
GCTACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACT	7	0.17500000000000002	No Hit
TTTATCACTGGAGGAAGGTGGGGATGACGTCAAGTCATCAT	7	0.17500000000000002	No Hit
GTCGCCCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAAT	7	0.17500000000000002	No Hit
GAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAAC	7	0.17500000000000002	No Hit
TCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGACT	7	0.17500000000000002	No Hit
TTCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTCCT	7	0.17500000000000002	No Hit
CTAGCCTGATTAAGTTTTAACGCTTCAACCCCAGGCAGGGC	6	0.15	No Hit
TTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACT	6	0.15	No Hit
TCTTTACATTCGCTTGCCAGCTGCGGACGGACACGCCACTA	6	0.15	No Hit
AACCCGCTGATTAAGCGGGTTTTGAATTCTTGCTGACGTAT	6	0.15	No Hit
TCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGT	6	0.15	No Hit
GTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTC	6	0.15	No Hit
ATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA	6	0.15	No Hit
TTTGCGTACTGTCAGAGCACTGATTTCTCTGACAGTACGTG	5	0.125	No Hit
TGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACTA	5	0.125	No Hit
AAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTC	5	0.125	No Hit
AAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAA	5	0.125	No Hit
GTGTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACC	5	0.125	No Hit
GACTCTCAAGGAACGTTGAAGACGACGACGTTGATAGGCCG	5	0.125	No Hit
GCTTAGTCAGTCTTTACATTCGCTTGCCAGCTGCGGACGGA	5	0.125	No Hit
AACAAACGGCACGACACAGCTGTCGTACCGTTATTGCTTAT	5	0.125	No Hit
TACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACTGG	5	0.125	No Hit
TTTAGCTACCGAGATGAGTTCTCCCTGACTCCTTGAGAGTC	5	0.125	No Hit
TTTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGAT	5	0.125	No Hit
TTTTAACGCTTCAACCCCAGGCAGGGCTTCCACGCGATCTC	5	0.125	No Hit
TATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.025	0.0	0.0	0.0	0.0
4	1.25	0.0	0.0	0.0	0.0
5	1.275	0.0	0.0	0.0	0.0
6	1.275	0.0	0.0	0.0	0.0
7	1.3	0.0	0.0	0.0	0.0
8	1.3	0.0	0.0	0.0	0.0
9	1.3	0.0	0.0	0.0	0.0
10	1.3	0.0	0.0	0.0	0.0
11	1.3	0.0	0.0	0.0	0.0
12	1.3	0.0	0.0	0.0	0.0
13	1.3	0.0	0.0	0.0	0.0
14	1.3	0.0	0.0	0.0	0.0
15	1.3	0.0	0.0	0.0	0.0
16	1.325	0.0	0.0	0.0	0.0
17	1.325	0.0	0.0	0.0	0.0
18	1.35	0.0	0.0	0.0	0.0
19	1.35	0.0	0.0	0.0	0.0
20	1.35	0.0	0.0	0.0	0.0
21	1.4	0.0	0.0	0.0	0.0
22	1.45	0.0	0.0	0.0	0.0
23	1.55	0.0	0.0	0.0	0.0
24	1.725	0.0	0.0	0.0	0.0
25	1.725	0.0	0.0	0.0	0.0
26	1.75	0.0	0.0	0.0	0.0
27	1.75	0.0	0.0	0.0	0.0
28	1.775	0.0	0.0	0.0	0.0
29	1.775	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTGGA	65	0.0	35.0	1
GGTGGAG	70	0.0	32.500004	2
TGGAGCT	80	0.0	28.4375	4
GTGGAGC	80	0.0	28.4375	3
GGAGCTG	85	1.8189894E-12	26.764704	5
GTTGAAC	90	3.6379788E-12	25.277779	18
GAAATTC	90	3.6379788E-12	25.277779	33
AAATTCC	90	3.6379788E-12	25.277779	34
AATTCCT	90	3.6379788E-12	25.277779	35
GTCCGAA	90	3.6379788E-12	25.277779	29
GAGCTGG	90	3.6379788E-12	25.277779	6
AACCCGC	90	3.6379788E-12	25.277779	22
ACCCGCG	90	3.6379788E-12	25.277779	23
GGGAGTT	90	3.6379788E-12	25.277779	14
GAGTTGA	90	3.6379788E-12	25.277779	16
CTGGCGG	90	3.6379788E-12	25.277779	9
CCCGCGT	90	3.6379788E-12	25.277779	24
GAACCCG	90	3.6379788E-12	25.277779	21
CGGGAGT	90	3.6379788E-12	25.277779	13
GCGTCCG	90	3.6379788E-12	25.277779	27
>>END_MODULE
SRR23332681 read2 length is 40-50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332681_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	40-50
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.6445	11.0	11.0	37.0	11.0	37.0
2	23.6255	25.0	11.0	37.0	11.0	37.0
3	25.444	25.0	11.0	37.0	11.0	37.0
4	36.129	37.0	37.0	37.0	37.0	37.0
5	36.298	37.0	37.0	37.0	37.0	37.0
6	36.1745	37.0	37.0	37.0	37.0	37.0
7	35.8965	37.0	37.0	37.0	37.0	37.0
8	35.989	37.0	37.0	37.0	37.0	37.0
9	35.823	37.0	37.0	37.0	37.0	37.0
10	36.0815	37.0	37.0	37.0	37.0	37.0
11	36.02	37.0	37.0	37.0	37.0	37.0
12	36.0415	37.0	37.0	37.0	37.0	37.0
13	36.1755	37.0	37.0	37.0	37.0	37.0
14	36.0905	37.0	37.0	37.0	37.0	37.0
15	36.0095	37.0	37.0	37.0	37.0	37.0
16	36.2435	37.0	37.0	37.0	37.0	37.0
17	36.2865	37.0	37.0	37.0	37.0	37.0
18	36.2635	37.0	37.0	37.0	37.0	37.0
19	36.2725	37.0	37.0	37.0	37.0	37.0
20	36.3	37.0	37.0	37.0	37.0	37.0
21	36.246	37.0	37.0	37.0	37.0	37.0
22	36.226	37.0	37.0	37.0	37.0	37.0
23	36.299	37.0	37.0	37.0	37.0	37.0
24	36.293	37.0	37.0	37.0	37.0	37.0
25	36.263	37.0	37.0	37.0	37.0	37.0
26	36.24575	37.0	37.0	37.0	37.0	37.0
27	36.0865	37.0	37.0	37.0	37.0	37.0
28	36.07525	37.0	37.0	37.0	37.0	37.0
29	36.213	37.0	37.0	37.0	37.0	37.0
30	36.33225	37.0	37.0	37.0	37.0	37.0
31	36.167	37.0	37.0	37.0	37.0	37.0
32	35.96475	37.0	37.0	37.0	37.0	37.0
33	36.207	37.0	37.0	37.0	37.0	37.0
34	36.19875	37.0	37.0	37.0	37.0	37.0
35	36.2315	37.0	37.0	37.0	37.0	37.0
36	36.2055	37.0	37.0	37.0	37.0	37.0
37	36.168	37.0	37.0	37.0	37.0	37.0
38	36.2	37.0	37.0	37.0	37.0	37.0
39	36.2735	37.0	37.0	37.0	37.0	37.0
40	36.1215	37.0	37.0	37.0	37.0	37.0
41	35.975743935984	37.0	37.0	37.0	37.0	37.0
42	36.13156578289144	37.0	37.0	37.0	37.0	37.0
43	36.04110275689223	37.0	37.0	37.0	37.0	37.0
44	36.05348542458809	37.0	37.0	37.0	37.0	37.0
45	36.05189941144997	37.0	37.0	37.0	37.0	37.0
46	36.008398807911135	37.0	37.0	37.0	37.0	37.0
47	35.994179600886916	37.0	37.0	37.0	37.0	37.0
48	36.09106382978723	37.0	37.0	37.0	37.0	37.0
49	36.11447920025798	37.0	37.0	37.0	37.0	37.0
50	35.9715821812596	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	0.0
19	1.0
20	2.0
21	4.0
22	2.0
23	7.0
24	8.0
25	7.0
26	16.0
27	18.0
28	17.0
29	19.0
30	26.0
31	53.0
32	74.0
33	162.0
34	391.0
35	1732.0
36	1215.0
37	245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	39.45	30.575000000000003	29.975
2	38.5	31.874999999999996	14.499999999999998	15.125
3	30.675	37.75	15.425	16.150000000000002
4	77.3	8.3	6.550000000000001	7.85
5	62.849999999999994	17.150000000000002	12.35	7.6499999999999995
6	56.00000000000001	19.6	11.025	13.375
7	36.725	38.3	10.225	14.75
8	39.025	18.975	15.65	26.35
9	20.974999999999998	37.15	28.725	13.15
10	41.05	23.0	18.325	17.625
11	26.8	40.8	17.95	14.45
12	19.25	26.875	31.2	22.675
13	32.025	14.899999999999999	33.725	19.35
14	31.075000000000003	17.125	25.1	26.700000000000003
15	26.900000000000002	20.025000000000002	42.375	10.7
16	45.824999999999996	21.325	22.0	10.85
17	28.599999999999998	33.025	18.224999999999998	20.150000000000002
18	16.775000000000002	41.8	25.650000000000002	15.775
19	20.349999999999998	19.525000000000002	35.8	24.325
20	16.175	23.875	32.125	27.825
21	18.775	40.025	15.024999999999999	26.174999999999997
22	40.1	28.499999999999996	14.725	16.675
23	22.525000000000002	32.824999999999996	20.225	24.425
24	23.200000000000003	22.8	14.649999999999999	39.35
25	39.725	18.15	13.875000000000002	28.249999999999996
26	35.50887721930482	21.305326331582897	14.50362590647662	28.68217054263566
27	37.875	24.3	21.875	15.950000000000001
28	28.7215411558669	28.04603452589442	28.796597448086064	14.435826870152615
29	22.2	16.275000000000002	47.225	14.299999999999999
30	18.313735301476108	18.263697773329998	50.96322241681261	12.459344508381285
31	19.3	22.5	40.1	18.099999999999998
32	34.57593194896172	15.31148361270953	20.740555416562422	29.372029021766327
33	23.625	16.175	14.549999999999999	45.65
34	28.021015761821367	21.09081811358519	21.966474856142106	28.921691268451337
35	13.275	39.425	22.675	24.625
36	12.331165582791396	40.495247623811906	29.264632316158078	17.90895447723862
37	18.025	44.3	13.200000000000001	24.474999999999998
38	20.125	21.4	11.525	46.949999999999996
39	31.874999999999996	18.75	12.85	36.525
40	19.975	22.475	20.3	37.25
41	17.829457364341085	42.385596399099775	24.256064016004	15.528882220555138
42	21.11055527763882	30.7903951975988	28.31415707853927	19.78489244622311
43	33.40852130325814	22.581453634085214	16.967418546365913	27.04260651629073
44	41.039290240811155	16.806083650190114	19.82256020278834	22.332065906210392
45	18.96736222578919	16.63991439272338	36.597110754414125	27.7956126270733
46	32.64697913844486	20.509347060417234	28.447575182877273	18.396098618260634
47	27.474355420016632	18.436373717771	24.89603548655392	29.193235375658443
48	25.900709219858154	27.00709219858156	26.382978723404253	20.70921985815603
49	30.590513068731852	12.681510164569215	41.04549854791868	15.682478218780252
50	34.331797235023046	14.055299539170507	25.49923195084485	26.113671274961597
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	1.0
20	1.0
21	0.5
22	0.0
23	1.5
24	3.5
25	5.0
26	6.5
27	8.0
28	9.0
29	16.5
30	27.5
31	42.0
32	62.5
33	77.0
34	82.0
35	95.0
36	109.0
37	107.5
38	98.5
39	104.5
40	117.5
41	107.0
42	101.0
43	115.5
44	127.5
45	143.5
46	159.5
47	168.5
48	191.0
49	218.5
50	238.0
51	380.5
52	517.5
53	674.0
54	846.5
55	828.0
56	735.0
57	552.5
58	395.5
59	256.0
60	133.0
61	93.0
62	64.5
63	46.5
64	26.0
65	17.0
66	12.0
67	10.0
68	8.5
69	6.5
70	3.5
71	1.5
72	1.5
73	1.5
74	0.5
75	1.0
76	2.0
77	2.5
78	3.0
79	1.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.025
27	0.0
28	0.075
29	0.0
30	0.075
31	0.0
32	0.075
33	0.0
34	0.075
35	0.0
36	0.05
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.02771618625277162
48	0.0
49	0.06449532408900355
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
40	1.0
41	1.0
42	8.0
43	45.0
44	207.0
45	47.0
46	83.0
47	83.0
48	424.0
49	497.0
50	2604.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.18518518518519	51.175000000000004
2	6.075738660008323	7.3
3	2.4136496046608404	4.35
4	1.4981273408239701	3.5999999999999996
5	0.9155222638368706	2.75
6	0.7906783187682065	2.85
7	0.6242197253433208	2.625
8	0.37453183520599254	1.7999999999999998
9	0.24968789013732834	1.35
>10	1.7894298793175198	18.95
>50	0.08322929671244278	3.25
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	79	1.975	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	51	1.275	No Hit
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	45	1.125	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	37	0.9249999999999999	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	34	0.8500000000000001	No Hit
CAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	33	0.8250000000000001	No Hit
AAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	33	0.8250000000000001	No Hit
AAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	31	0.775	No Hit
CTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	25	0.625	No Hit
TAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	24	0.6	No Hit
CGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	23	0.575	No Hit
ATTACCACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACGT	23	0.575	Illumina Single End PCR Primer 1 (97% over 34bp)
TGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	21	0.525	No Hit
CGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	20	0.5	No Hit
TGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	20	0.5	No Hit
TGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	18	0.44999999999999996	No Hit
AATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	17	0.42500000000000004	No Hit
TACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
CGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
CCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
ACAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
AGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
CGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
ACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
CCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
ACCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
CAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
AACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
AGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
TGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
CATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
CGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
TGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	12	0.3	No Hit
ATAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
CACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
CGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
TAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
CGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
AAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
TGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
AGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	10	0.25	No Hit
AATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
TGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
TAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
CAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
ATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
TGTGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
TCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
ACCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
TTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTA	7	0.17500000000000002	No Hit
AATGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
CGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TTTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
AACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
ATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
ACTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
ACAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
ATAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
TGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
ATAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TTTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
AGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CCCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
AAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGG	6	0.15	No Hit
TGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
CGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
AAAGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGG	6	0.15	No Hit
TTTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
AAAGGATCAATTTTCATCCGAACGTTCCTGACAGGAGAAAACCATGAGTA	5	0.125	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTG	5	0.125	No Hit
ACGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
AGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CCTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TATGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
ATCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CTCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
ACAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CACGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
AAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
AGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAT	5	0.125	No Hit
AACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.025	0.0	0.0	0.0	0.0
10	0.625	0.0	0.0	0.0	0.0
11	0.7	0.0	0.0	0.0	0.0
12	0.75	0.0	0.0	0.0	0.0
13	1.275	0.0	0.0	0.0	0.0
14	1.275	0.0	0.0	0.0	0.0
15	1.275	0.0	0.0	0.0	0.0
16	1.3	0.0	0.0	0.0	0.0
17	1.3	0.0	0.0	0.0	0.0
18	1.3	0.0	0.0	0.0	0.0
19	1.3	0.0	0.0	0.0	0.0
20	1.3	0.0	0.0	0.0	0.0
21	1.3	0.0	0.0	0.0	0.0
22	1.3	0.0	0.0	0.0	0.0
23	1.375	0.0	0.0	0.0	0.0
24	1.375	0.0	0.0	0.0	0.0
25	1.4	0.0	0.0	0.0	0.0
26	1.4	0.0	0.0	0.0	0.0
27	1.45	0.0	0.0	0.0	0.0
28	1.475	0.0	0.0	0.0	0.0
29	1.5	0.0	0.0	0.0	0.0
30	1.55	0.0	0.0	0.0	0.0
31	1.575	0.0	0.0	0.0	0.0
32	1.625	0.0	0.0	0.0	0.0
33	1.725	0.0	0.0	0.0	0.0
34	1.725	0.0	0.0	0.0	0.0
35	1.75	0.0	0.0	0.0	0.0
36	1.75	0.0	0.0	0.0	0.0
37	1.775	0.0	0.0	0.0	0.0
38	1.775	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCATG	90	0.0	57.844864	44
GCTGCGC	40	8.309289E-7	48.806602	44
GGTGCAT	95	0.0	47.613457	43
AGGTGCA	90	0.0	43.179966	42
AGGGGCT	30	2.2457007E-6	43.112503	3
AAGGGAG	30	2.2457007E-6	43.112503	2
AAGGGGC	20	7.686455E-4	43.1125	2
TGGGGCT	20	7.686455E-4	43.1125	3
AAAGGGA	25	4.1687366E-5	43.1125	1
CGCTGCG	40	2.3139237E-6	42.40574	43
AAGGTGC	90	0.0	41.996956	41
CAAGGTG	90	0.0	40.339184	40
CCAAGGT	90	0.0	40.339184	39
CCCAAGG	90	0.0	38.807316	38
AAACCCA	90	0.0	38.322224	35
TCGACGG	90	0.0	38.322224	20
GAAACCC	90	0.0	38.322224	34
TCTGGAT	90	0.0	38.322224	13
TGATTCT	90	0.0	38.322224	9
GACGGGA	90	0.0	38.322224	22
>>END_MODULE
Read 72412 spots for SRR23332681.sra
Written 72412 spots for SRR23332681.sra
Read 72412 spots for SRR23332681.sra
Written 72412 spots for SRR23332681.sra
Read 72412 spots for SRR23332681.sra
Written 72412 spots for SRR23332681.sra
Read 72412 spots for SRR23332681.sra
Written 72412 spots for SRR23332681.sra
Read 72412 spots for SRR23332681.sra
Written 72412 spots for SRR23332681.sra
Read 72414 spots for SRR23332681.sra
Written 72414 spots for SRR23332681.sra
Read 72412 spots for SRR23332681.sra
Written 72412 spots for SRR23332681.sra
Read 72412 spots for SRR23332681.sra
Written 72412 spots for SRR23332681.sra
SRR ids: ['SRR23332681.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_oov0za7v
SRR23332681.sra spots: 579298
blocks: [[1, 72412], [72413, 144824], [144825, 217236], [217237, 289648], [289649, 362060], [362061, 434472], [434473, 506884], [506885, 579298]]
SRR23332681 file size 69368
SRR23332681 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR23332681 SRR23332681_1.fastq SRR23332681_2.fastq
Input file:	SRR23332681_1.fastq
Paired file:	SRR23332681_2.fastq
trimmed:	SRR23332681-trimmed-pair1.fastq, SRR23332681-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 04:28:36 2026 >> started

Wed Mar 25 04:28:37 2026 >> done (0.463s)
579298 read pairs processed; of these:
    87 ( 0.02%) short read pairs filtered out after trimming by size control
     3 ( 0.00%) empty read pairs filtered out after trimming by size control
579208 (99.98%) read pairs available; of these:
  3959 ( 0.68%) trimmed read pairs available after processing
575249 (99.32%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    16	  0.00%
 19	    31	  0.01%
 20	    84	  0.01%
 21	    49	  0.01%
 22	    52	  0.01%
 23	    44	  0.01%
 24	    65	  0.01%
 25	    84	  0.01%
 26	    59	  0.01%
 27	    87	  0.02%
 28	    64	  0.01%
 29	   108	  0.02%
 30	    78	  0.01%
 31	    84	  0.01%
 32	   114	  0.02%
 33	   139	  0.02%
 34	   178	  0.03%
 35	   212	  0.04%
 36	   220	  0.04%
 37	   222	  0.04%
 38	   350	  0.06%
 39	   329	  0.06%
 40	   846	  0.15%
 41	   865	  0.15%
 42	 29561	  5.10%
 43	 15512	  2.68%
 44	 61756	 10.66%
 45	467999	 80.80%
579208 reads passed initial QC


criterion=sequence-density
sequence-density=1.09
sequence-density-rank=1
fanout-score=1.99
fanout-score-rank=18
prefix-density=0.96
prefix-fanout=2.0
sequence=GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCG


criterion=fanout-score
sequence-density=0.27
sequence-density-rank=15
fanout-score=7.20
fanout-score-rank=1
prefix-density=1.98
prefix-fanout=1.0
sequence=TTCATTAGTACGAGAGGACCGGGATGGACGCAC


criterion=sequence-density
sequence-density=17.95
sequence-density-rank=1
fanout-score=21.87
fanout-score-rank=5
prefix-density=14.77
prefix-fanout=21.9
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGG


criterion=fanout-score
sequence-density=0.73
sequence-density-rank=12
fanout-score=139.99
fanout-score-rank=1
prefix-density=8.71
prefix-fanout=11.8
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTATTACCACG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCG -y GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGG -o SRR23332681 SRR23332681_1.fastq SRR23332681_2.fastq
Input file:	SRR23332681_1.fastq
Paired file:	SRR23332681_2.fastq
trimmed:	SRR23332681-trimmed-pair1.fastq, SRR23332681-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAG
-- paired 3' end adapter sequence (-y):	GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 04:28:39 2026 >> started

Wed Mar 25 04:28:39 2026 >> done (0.388s)
463367 read pairs processed; of these:
  4683 ( 1.01%) short read pairs filtered out after trimming by size control
   555 ( 0.12%) empty read pairs filtered out after trimming by size control
458129 (98.87%) read pairs available; of these:
   166 ( 0.04%) trimmed read pairs available after processing
457963 (99.96%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    11	  0.00%
 19	    24	  0.01%
 20	    62	  0.01%
 21	    37	  0.01%
 22	    41	  0.01%
 23	    33	  0.01%
 24	    50	  0.01%
 25	    72	  0.02%
 26	    45	  0.01%
 27	    74	  0.02%
 28	    47	  0.01%
 29	    85	  0.02%
 30	    61	  0.01%
 31	    73	  0.02%
 32	    95	  0.02%
 33	   101	  0.02%
 34	   139	  0.03%
 35	   166	  0.04%
 36	   174	  0.04%
 37	   186	  0.04%
 38	   285	  0.06%
 39	   261	  0.06%
 40	   649	  0.14%
 41	   708	  0.15%
 42	 23434	  5.12%
 43	 12331	  2.69%
 44	 47885	 10.45%
 45	371000	 80.98%


criterion=sequence-density
sequence-density=1.03
sequence-density-rank=1
fanout-score=1.99
fanout-score-rank=18
prefix-density=0.97
prefix-fanout=2.0
sequence=GTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCG


criterion=fanout-score
sequence-density=0.28
sequence-density-rank=15
fanout-score=7.20
fanout-score-rank=1
prefix-density=2.00
prefix-fanout=1.0
sequence=TTCATTAGTACGAGAGGACCGGGATGGACGCAC


criterion=sequence-density
sequence-density=17.35
sequence-density-rank=1
fanout-score=21.63
fanout-score-rank=6
prefix-density=14.57
prefix-fanout=21.6
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGG


criterion=fanout-score
sequence-density=0.74
sequence-density-rank=12
fanout-score=139.99
fanout-score-rank=1
prefix-density=8.79
prefix-fanout=11.8
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTATTACCACG
SRR23332681 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 25 04:28:52
                             Started mapping on |	Mar 25 04:28:52
                                    Finished on |	Mar 25 04:29:33
       Mapping speed, Million of reads per hour |	50.40

                          Number of input reads |	573970
                      Average input read length |	90
                                    UNIQUE READS:
                   Uniquely mapped reads number |	344414
                        Uniquely mapped reads % |	60.01%
                          Average mapped length |	87.70
                       Number of splices: Total |	14166
            Number of splices: Annotated (sjdb) |	22
                       Number of splices: GT/AG |	12672
                       Number of splices: GC/AG |	298
                       Number of splices: AT/AC |	67
               Number of splices: Non-canonical |	1129
                      Mismatch rate per base, % |	0.24%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.22
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	100001
             % of reads mapped to multiple loci |	17.42%
        Number of reads mapped to too many loci |	92613
             % of reads mapped to too many loci |	16.14%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.07%
                     % of reads unmapped: other |	0.37%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	129556	129556	129556
N_multimapping	100001	100001	100001
N_noFeature	16259	318431	26905
N_ambiguous	26832	3580	9210
UnstrandedReadsAssigned:301323 PositiveStrandReadsAssigned:22403 NegativeStrandReadsAssigned:308299
Dataset is classified negative stranded
MeadianReadLen=41 20thPercentileLength=41 echo kmer=37
SRR23332681 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR23332681-trimmed-pair1.fastq
                             SRR23332681-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 573,970 reads, 124,677 reads pseudoaligned
[quant] estimated average fragment length: 204.825
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 52 rounds

  4323 SRR23332681.ke.tsv
  4497 SRR23332681.se.tsv
  8820 total
==> SRR23332681.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	10.5678	24	2449.43
AAC73113	2463	2259.18	14	6.6837
AAC73114	933	729.175	3	4.43739
AAC73115	1287	1083.18	13	12.9444
AAC73116	297	119.135	0	0
AAC73117	777	573.418	1	1.88091
AAC73118	1431	1227.18	7	6.15219
AAC73119	954	750.175	52	74.7617
AAC73120	588	385.755	4	11.1837

==> SRR23332681.se.tsv <==
b0001	53
b0002	14
b0003	1
b0004	11
b0005	0
b0006	8
b0007	7
b0008	51
b0009	4
b0010	5
SRR23332681 completed mapping pipeline successfully
