Starting /dee2/code/volunteer_pipeline.sh SRR23332720
    current disk space = 1460711584
    free memory = 58646112 
SRR23332720 SRAfilesize
4bf49b4addfc7a179c7fd2f08eb59daa  SRR23332720.sra
SRR23332720.sra file validated
SRR23332720 is paired end
SRR23332720 is conventional basespace
SRR23332720 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332720_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.5345	37.0	37.0	37.0	37.0	37.0
2	36.518	37.0	37.0	37.0	37.0	37.0
3	36.465	37.0	37.0	37.0	37.0	37.0
4	36.4485	37.0	37.0	37.0	37.0	37.0
5	36.389	37.0	37.0	37.0	37.0	37.0
6	36.3855	37.0	37.0	37.0	37.0	37.0
7	36.349	37.0	37.0	37.0	37.0	37.0
8	36.4415	37.0	37.0	37.0	37.0	37.0
9	36.5285	37.0	37.0	37.0	37.0	37.0
10	36.4755	37.0	37.0	37.0	37.0	37.0
11	36.4775	37.0	37.0	37.0	37.0	37.0
12	36.3375	37.0	37.0	37.0	37.0	37.0
13	36.482	37.0	37.0	37.0	37.0	37.0
14	36.4905	37.0	37.0	37.0	37.0	37.0
15	36.547	37.0	37.0	37.0	37.0	37.0
16	36.4865	37.0	37.0	37.0	37.0	37.0
17	36.407	37.0	37.0	37.0	37.0	37.0
18	36.3605	37.0	37.0	37.0	37.0	37.0
19	36.451	37.0	37.0	37.0	37.0	37.0
20	36.4125	37.0	37.0	37.0	37.0	37.0
21	36.4225	37.0	37.0	37.0	37.0	37.0
22	36.3235	37.0	37.0	37.0	37.0	37.0
23	36.271	37.0	37.0	37.0	37.0	37.0
24	36.3325	37.0	37.0	37.0	37.0	37.0
25	36.392	37.0	37.0	37.0	37.0	37.0
26	36.3205	37.0	37.0	37.0	37.0	37.0
27	36.365	37.0	37.0	37.0	37.0	37.0
28	36.486	37.0	37.0	37.0	37.0	37.0
29	36.4185	37.0	37.0	37.0	37.0	37.0
30	36.33	37.0	37.0	37.0	37.0	37.0
31	36.41	37.0	37.0	37.0	37.0	37.0
32	36.3685	37.0	37.0	37.0	37.0	37.0
33	36.437	37.0	37.0	37.0	37.0	37.0
34	36.2715	37.0	37.0	37.0	37.0	37.0
35	36.3705	37.0	37.0	37.0	37.0	37.0
36	36.331	37.0	37.0	37.0	37.0	37.0
37	36.2515	37.0	37.0	37.0	37.0	37.0
38	36.3795	37.0	37.0	37.0	37.0	37.0
39	36.2675	37.0	37.0	37.0	37.0	37.0
40	36.412	37.0	37.0	37.0	37.0	37.0
41	36.3715	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	2.0
26	3.0
27	14.0
28	18.0
29	17.0
30	26.0
31	20.0
32	47.0
33	81.0
34	108.0
35	232.0
36	1216.0
37	2216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.224999999999998	19.225	43.275000000000006	10.274999999999999
2	26.900000000000002	18.075	29.549999999999997	25.474999999999998
3	26.875	20.4	30.9	21.825
4	21.3	22.925	34.65	21.125
5	29.4	22.175	24.175	24.25
6	32.375	21.625	21.675	24.325
7	24.05	29.4	22.0	24.55
8	34.225	18.675	20.674999999999997	26.424999999999997
9	26.200000000000003	16.950000000000003	22.15	34.699999999999996
10	26.674999999999997	17.8	28.9	26.625
11	35.275	18.95	19.650000000000002	26.125
12	33.825	19.650000000000002	21.425	25.1
13	27.200000000000003	20.05	22.650000000000002	30.099999999999998
14	33.75	22.25	22.725	21.275
15	33.5	21.15	23.3	22.05
16	35.199999999999996	22.875	21.975	19.950000000000003
17	26.174999999999997	29.299999999999997	24.15	20.375
18	32.025	21.349999999999998	24.625	22.0
19	25.95	22.375	30.075000000000003	21.6
20	26.8	22.3	28.475	22.425
21	33.5	21.349999999999998	20.825	24.325
22	26.3	28.175	21.575	23.95
23	26.05	29.45	21.45	23.05
24	24.975	20.875	21.625	32.525
25	28.725	19.275000000000002	20.599999999999998	31.4
26	25.55	20.200000000000003	23.7	30.55
27	34.375	21.95	20.775	22.900000000000002
28	24.45	20.599999999999998	22.125	32.824999999999996
29	34.2	19.375	23.275000000000002	23.150000000000002
30	25.324999999999996	20.65	31.525	22.5
31	24.55	22.650000000000002	22.225	30.575000000000003
32	26.35	22.075	20.95	30.625000000000004
33	33.425	21.9	22.875	21.8
34	24.224999999999998	30.175	21.7	23.9
35	23.1	31.75	22.225	22.925
36	22.725	30.175	22.275	24.825
37	24.85	22.225	29.675	23.25
38	24.775	21.875	29.049999999999997	24.3
39	24.725	22.375	21.875	31.025000000000002
40	24.349999999999998	23.025000000000002	21.075	31.55
41	23.325000000000003	22.15	30.0	24.525
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	1.0
20	1.0
21	0.5
22	0.0
23	0.5
24	1.0
25	1.0
26	1.0
27	1.0
28	5.0
29	9.0
30	15.0
31	21.0
32	21.0
33	23.0
34	25.0
35	31.5
36	38.0
37	38.0
38	54.0
39	70.0
40	112.0
41	154.0
42	154.0
43	194.0
44	234.0
45	289.0
46	344.0
47	344.0
48	382.0
49	420.0
50	457.5
51	495.0
52	521.5
53	548.0
54	548.0
55	492.0
56	436.0
57	392.5
58	349.0
59	349.0
60	475.5
61	602.0
62	367.5
63	133.0
64	133.0
65	100.5
66	68.0
67	47.5
68	27.0
69	27.0
70	23.0
71	19.0
72	11.5
73	4.0
74	2.5
75	1.0
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.70534069981584	54.1
2	14.438305709023942	19.6
3	2.9097605893186005	5.925
4	1.3259668508287292	3.5999999999999996
5	0.44198895027624313	1.5
6	0.3314917127071823	1.35
7	0.29465930018416203	1.4000000000000001
8	0.07366482504604051	0.4
9	0.07366482504604051	0.44999999999999996
>10	0.3683241252302026	3.5999999999999996
>50	0.0	0.0
>100	0.036832412523020254	8.075000000000001
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCT	323	8.075000000000001	No Hit
GGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTA	23	0.575	No Hit
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTAC	19	0.475	No Hit
AGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGT	16	0.4	No Hit
GGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAAC	15	0.375	No Hit
TTATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGAA	14	0.35000000000000003	No Hit
TTTATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGA	13	0.325	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCC	12	0.3	No Hit
AAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGAG	11	0.27499999999999997	No Hit
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCT	11	0.27499999999999997	TruSeq Adapter, Index 8 (97% over 37bp)
GCTACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACT	10	0.25	No Hit
AGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAA	9	0.22499999999999998	No Hit
GGTGTGTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC	9	0.22499999999999998	No Hit
GGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAACC	8	0.2	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCC	8	0.2	No Hit
TGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACTA	7	0.17500000000000002	No Hit
TATGAGGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCC	7	0.17500000000000002	No Hit
TCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGT	7	0.17500000000000002	No Hit
GGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACAT	7	0.17500000000000002	No Hit
TACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACTGG	7	0.17500000000000002	No Hit
TTTGAGTTCCCGGGCCTTGTACACACCGCCCGTCACACCAT	7	0.17500000000000002	No Hit
TCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGACT	7	0.17500000000000002	No Hit
GGGTTTGACCTCTCTTGATCCCCGTCCTAAGAGCGGAGGCT	7	0.17500000000000002	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACGAGAGGACCGGGA	6	0.15	No Hit
GCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACTAC	6	0.15	No Hit
GGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTC	6	0.15	No Hit
GTTTGACCTCTCTTGATCCCCGTCCTAAGAGCGGAGGCTAG	6	0.15	No Hit
TGTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCT	6	0.15	No Hit
TCATGGAGTCGAGTTGCTAGTAATCGTGGATCAGAATGCCA	6	0.15	No Hit
ATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCA	6	0.15	No Hit
TTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCAC	6	0.15	No Hit
TGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACA	6	0.15	No Hit
GCGGAGGCTAGGGAGAGAGGGCTCTAAGCAGGTTATTAAGC	5	0.125	No Hit
AAAAAAATGGCGCACAATGTGCGCCATTTTTCACTTCACAG	5	0.125	No Hit
GAGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGG	5	0.125	No Hit
TTTCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTCC	5	0.125	No Hit
ACTTTATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCAT	5	0.125	No Hit
GACGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCT	5	0.125	No Hit
TTATCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTG	5	0.125	No Hit
TCAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGT	5	0.125	No Hit
TCGTTGCGGGACTTAACCTTCGGGAGGGCGCTTACCACTTT	5	0.125	No Hit
GTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTC	5	0.125	No Hit
GTTAAGGGGGATAACCTCCCCCTCAGGTAAAAATTATTCTG	5	0.125	No Hit
TGAGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.025	0.0	0.0	0.0	0.0
3	0.05	0.0	0.0	0.0	0.0
4	0.35	0.0	0.0	0.0	0.0
5	0.4	0.0	0.0	0.0	0.0
6	0.4	0.0	0.0	0.0	0.0
7	0.4	0.0	0.0	0.0	0.0
8	0.4	0.0	0.0	0.0	0.0
9	0.4	0.0	0.0	0.0	0.0
10	0.4	0.0	0.0	0.0	0.0
11	0.4	0.0	0.0	0.0	0.0
12	0.4	0.0	0.0	0.0	0.0
13	0.4	0.0	0.0	0.0	0.0
14	0.45	0.0	0.0	0.0	0.0
15	0.45	0.0	0.0	0.0	0.0
16	0.475	0.0	0.0	0.0	0.0
17	0.475	0.0	0.0	0.0	0.0
18	0.55	0.0	0.0	0.0	0.0
19	0.575	0.0	0.0	0.0	0.0
20	0.65	0.0	0.0	0.0	0.0
21	0.675	0.0	0.0	0.0	0.0
22	0.75	0.0	0.0	0.0	0.0
23	0.8	0.0	0.0	0.0	0.0
24	0.825	0.0	0.0	0.0	0.0
25	0.875	0.0	0.0	0.0	0.0
26	0.95	0.0	0.0	0.0	0.0
27	1.075	0.0	0.0	0.0	0.0
28	1.15	0.0	0.0	0.0	0.0
29	1.175	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTGGAG	30	9.417061E-6	35.000004	2
TGGTGGA	30	9.417061E-6	35.000004	1
GTGGAGC	45	1.5026014E-4	23.333334	3
GCTGGCG	50	3.0707463E-4	21.0	8
GAGCTGG	50	3.0707463E-4	21.0	6
TGGAGCT	50	3.0707463E-4	21.0	4
CTGGCGG	50	3.0707463E-4	21.0	9
TGGCGGG	50	3.0707463E-4	21.0	10
AGCTGGC	50	3.0707463E-4	21.0	7
CGCGTCC	60	4.108416E-5	20.416668	26
GCGGGAG	55	5.849127E-4	19.090908	12
GAAATTC	55	5.849127E-4	19.090908	33
AAATTCC	55	5.849127E-4	19.090908	34
AATTCCT	55	5.849127E-4	19.090908	35
GTCCGAA	55	5.849127E-4	19.090908	29
ACCCGCG	55	5.849127E-4	19.090908	23
GGGAGTT	55	5.849127E-4	19.090908	14
GAGTTGA	55	5.849127E-4	19.090908	16
CCCGCGT	55	5.849127E-4	19.090908	24
GAACCCG	55	5.849127E-4	19.090908	21
>>END_MODULE
SRR23332720 read2 length is 36-50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332720_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	36-50
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	19.743	11.0	11.0	37.0	11.0	37.0
2	22.78	25.0	11.0	37.0	11.0	37.0
3	25.615	25.0	11.0	37.0	11.0	37.0
4	35.6125	37.0	37.0	37.0	37.0	37.0
5	36.035	37.0	37.0	37.0	37.0	37.0
6	36.0	37.0	37.0	37.0	37.0	37.0
7	35.5785	37.0	37.0	37.0	37.0	37.0
8	35.936	37.0	37.0	37.0	37.0	37.0
9	35.671	37.0	37.0	37.0	37.0	37.0
10	35.9805	37.0	37.0	37.0	37.0	37.0
11	35.879	37.0	37.0	37.0	37.0	37.0
12	35.957	37.0	37.0	37.0	37.0	37.0
13	35.839	37.0	37.0	37.0	37.0	37.0
14	36.072	37.0	37.0	37.0	37.0	37.0
15	35.829	37.0	37.0	37.0	37.0	37.0
16	35.884	37.0	37.0	37.0	37.0	37.0
17	35.917	37.0	37.0	37.0	37.0	37.0
18	36.087	37.0	37.0	37.0	37.0	37.0
19	36.0265	37.0	37.0	37.0	37.0	37.0
20	35.936	37.0	37.0	37.0	37.0	37.0
21	35.97475	37.0	37.0	37.0	37.0	37.0
22	36.082	37.0	37.0	37.0	37.0	37.0
23	36.1125	37.0	37.0	37.0	37.0	37.0
24	36.157	37.0	37.0	37.0	37.0	37.0
25	36.06	37.0	37.0	37.0	37.0	37.0
26	36.019	37.0	37.0	37.0	37.0	37.0
27	35.93925	37.0	37.0	37.0	37.0	37.0
28	36.04425	37.0	37.0	37.0	37.0	37.0
29	35.997	37.0	37.0	37.0	37.0	37.0
30	36.01025	37.0	37.0	37.0	37.0	37.0
31	36.121	37.0	37.0	37.0	37.0	37.0
32	35.89275	37.0	37.0	37.0	37.0	37.0
33	35.84625	37.0	37.0	37.0	37.0	37.0
34	35.92875	37.0	37.0	37.0	37.0	37.0
35	36.1145	37.0	37.0	37.0	37.0	37.0
36	36.05125	37.0	37.0	37.0	37.0	37.0
37	36.05076269067267	37.0	37.0	37.0	37.0	37.0
38	35.959739934983745	37.0	37.0	37.0	37.0	37.0
39	36.124281070267564	37.0	37.0	37.0	37.0	37.0
40	36.019009504752376	37.0	37.0	37.0	37.0	37.0
41	36.008504252126066	37.0	37.0	37.0	37.0	37.0
42	35.95347673836918	37.0	37.0	37.0	37.0	37.0
43	35.75513269904857	37.0	37.0	37.0	37.0	37.0
44	35.81653225806452	37.0	37.0	37.0	37.0	37.0
45	35.818658280922435	37.0	37.0	37.0	37.0	37.0
46	35.84566596194503	37.0	37.0	37.0	37.0	37.0
47	35.791466666666665	37.0	37.0	37.0	37.0	37.0
48	35.86930746429534	37.0	37.0	37.0	37.0	37.0
49	35.895783847981	37.0	37.0	37.0	37.0	37.0
50	35.80977054034049	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	3.0
17	0.0
18	3.0
19	2.0
20	3.0
21	10.0
22	6.0
23	7.0
24	10.0
25	10.0
26	13.0
27	23.0
28	26.0
29	48.0
30	50.0
31	53.0
32	76.0
33	161.0
34	401.0
35	1737.0
36	1203.0
37	154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	38.3	23.200000000000003	38.5
2	30.599999999999998	32.425	14.499999999999998	22.475
3	30.925000000000004	40.8	10.375	17.9
4	79.95	6.950000000000001	5.5	7.6
5	66.75	16.425	9.675	7.1499999999999995
6	56.474999999999994	21.5	9.125	12.9
7	35.425000000000004	42.575	10.25	11.75
8	45.074999999999996	22.325	12.55	20.05
9	21.975	42.325	21.875	13.825000000000001
10	40.525	23.25	17.7	18.525
11	31.55	37.95	16.0	14.499999999999998
12	26.125	26.924999999999997	21.85	25.1
13	42.3	13.600000000000001	24.675	19.425
14	37.425000000000004	14.899999999999999	27.200000000000003	20.474999999999998
15	28.775000000000002	18.525	40.975	11.725
16	45.275	21.15	21.575	12.0
17	23.225	35.9	18.65	22.225
18	16.55	34.699999999999996	30.075000000000003	18.675
19	21.4	17.5	31.8	29.299999999999997
20	16.2	24.025	28.299999999999997	31.474999999999998
21	19.154788697174293	44.836209052263065	13.4783695923981	22.53063265816454
22	37.875	29.9	13.850000000000001	18.375
23	23.05	25.6	20.25	31.1
24	25.575	21.85	12.950000000000001	39.625
25	37.824999999999996	16.55	13.05	32.574999999999996
26	29.575000000000003	21.875	15.049999999999999	33.5
27	37.634408602150536	26.331582895723933	20.955238809702426	15.078769692423105
28	36.25906476619154	22.005501375343837	26.756689172293076	14.978744686171543
29	26.150000000000002	14.975	43.05	15.825
30	21.080270067516878	16.754188547136785	48.66216554138534	13.50337584396099
31	18.224999999999998	22.675	37.65	21.45
32	29.182295573893473	12.978244561140285	21.705426356589147	36.134033508377094
33	22.355588897224308	13.278319579894973	16.204051012753187	48.16204051012753
34	21.99149362021516	17.538153615211407	22.61696272204153	37.8533900425319
35	13.575000000000001	29.825000000000003	23.1	33.5
36	14.003500875218805	36.60915228807202	30.582645661415352	18.804701175293822
37	17.179294823705927	43.71092773193298	12.653163290822706	26.456614153538382
38	24.006001500375092	19.454863715928983	11.577894473618406	44.96124031007752
39	40.46011502875719	16.854213553388348	13.553388347086774	29.132283070767688
40	25.56278139069535	20.68534267133567	20.035017508754375	33.71685842921461
41	18.05902951475738	36.04302151075538	30.69034517258629	15.207603801900952
42	20.735367683841922	26.138069034517258	31.390695347673837	21.735867933966986
43	28.31747621432148	24.41161742613921	15.973960941412118	31.29694541812719
44	35.534274193548384	13.911290322580644	20.262096774193548	30.29233870967742
45	17.269392033542978	14.727463312368974	33.490566037735846	34.5125786163522
46	26.691331923890065	18.921775898520085	35.14799154334038	19.23890063424947
47	31.119999999999997	14.08	28.986666666666665	25.813333333333333
48	30.12665049851792	21.1802748585287	27.02775532201563	21.665319320937755
49	30.175230175230176	12.384912384912385	40.213840213840214	17.226017226017227
50	25.536639526276833	15.210954848260547	29.533678756476682	29.718726868985932
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	1.0
8	1.0
9	0.5
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	1.0
16	2.0
17	1.0
18	0.0
19	0.5
20	1.5
21	2.0
22	2.0
23	2.5
24	3.0
25	3.5
26	5.0
27	6.5
28	6.0
29	7.0
30	11.5
31	20.0
32	27.0
33	27.5
34	28.5
35	32.5
36	38.5
37	44.5
38	43.5
39	54.0
40	72.0
41	78.5
42	80.0
43	81.0
44	88.5
45	111.0
46	135.5
47	161.0
48	177.0
49	185.0
50	210.0
51	355.0
52	496.5
53	634.0
54	779.0
55	813.5
56	812.0
57	698.0
58	559.5
59	401.5
60	255.0
61	157.0
62	84.5
63	73.5
64	63.5
65	50.5
66	37.0
67	27.0
68	17.5
69	10.0
70	5.5
71	5.5
72	5.0
73	3.5
74	3.5
75	3.0
76	2.0
77	1.5
78	1.0
79	1.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.5
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.025
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.025
28	0.025
29	0.0
30	0.025
31	0.0
32	0.025
33	0.025
34	0.075
35	0.0
36	0.025
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.02969121140142518
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
36	1.0
37	0.0
38	0.0
39	1.0
40	0.0
41	0.0
42	4.0
43	26.0
44	152.0
45	32.0
46	34.0
47	39.0
48	343.0
49	666.0
50	2702.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.7
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.79904306220095	55.05
2	4.106858054226476	5.1499999999999995
3	2.073365231259968	3.9
4	0.9968102073365231	2.5
5	0.9968102073365231	3.125
6	1.036682615629984	3.9
7	0.518341307814992	2.275
8	0.7974481658692184	4.0
9	0.23923444976076555	1.35
>10	1.3157894736842104	14.299999999999999
>50	0.11961722488038277	4.45
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	69	1.725	No Hit
AAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	56	1.4000000000000001	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	53	1.325	No Hit
AAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	45	1.125	No Hit
AAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	32	0.8	No Hit
CGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	27	0.675	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	26	0.65	No Hit
CAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	25	0.625	No Hit
CCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	24	0.6	No Hit
ACAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	22	0.5499999999999999	No Hit
CGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	21	0.525	No Hit
CGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	21	0.525	No Hit
CAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	20	0.5	No Hit
CCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	20	0.5	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	19	0.475	No Hit
ACAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	18	0.44999999999999996	No Hit
CCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	17	0.42500000000000004	No Hit
CCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	16	0.4	No Hit
ACAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	16	0.4	No Hit
CAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	16	0.4	No Hit
CTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
CCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
TGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	14	0.35000000000000003	No Hit
CCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	13	0.325	No Hit
CGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
CGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	12	0.3	No Hit
TGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	11	0.27499999999999997	No Hit
TAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	11	0.27499999999999997	No Hit
TAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
CGCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	11	0.27499999999999997	No Hit
AGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
CCCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	11	0.27499999999999997	No Hit
ATAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
CACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
ACAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	10	0.25	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTA	9	0.22499999999999998	No Hit
AAAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	9	0.22499999999999998	No Hit
CCCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	9	0.22499999999999998	No Hit
CCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
TTCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
AGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
AATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
CAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
TGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
AGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
TAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
CCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CGAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
CTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
AAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
ATAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
AACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TTAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
AATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AAAGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCT	7	0.17500000000000002	No Hit
AGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
AACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
CGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
CGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
ATAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
TAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CCTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
CGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AAAGGGAGAGTCCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCA	6	0.15	No Hit
CTCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
ACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
ACCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AAAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGT	6	0.15	No Hit
AGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	6	0.15	No Hit
ATAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
ACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
AACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
ATTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CACGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
AAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CCGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TTGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TTAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AGGTCCTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGAG	5	0.125	Illumina Single End PCR Primer 1 (97% over 34bp)
AAAGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	5	0.125	No Hit
CGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CCAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TTGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
ACAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGT	5	0.125	No Hit
TTTGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TAGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
ACCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
ACTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CTGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
AGAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.025	0.0	0.0	0.0	0.0
10	0.175	0.0	0.0	0.0	0.0
11	0.25	0.0	0.0	0.0	0.0
12	0.3	0.0	0.0	0.0	0.0
13	0.375	0.0	0.0	0.0	0.0
14	0.4	0.0	0.0	0.0	0.0
15	0.4	0.0	0.0	0.0	0.0
16	0.4	0.0	0.0	0.0	0.0
17	0.4	0.0	0.0	0.0	0.0
18	0.4	0.0	0.0	0.0	0.0
19	0.4	0.0	0.0	0.0	0.0
20	0.425	0.0	0.0	0.0	0.0
21	0.45	0.0	0.0	0.0	0.0
22	0.475	0.0	0.0	0.0	0.0
23	0.5	0.0	0.0	0.0	0.0
24	0.5	0.0	0.0	0.0	0.0
25	0.5	0.0	0.0	0.0	0.0
26	0.5	0.0	0.0	0.0	0.0
27	0.575	0.0	0.0	0.0	0.0
28	0.625	0.0	0.0	0.0	0.0
29	0.7	0.0	0.0	0.0	0.0
30	0.775	0.0	0.0	0.0	0.0
31	0.85	0.0	0.0	0.0	0.0
32	0.875	0.0	0.0	0.0	0.0
33	0.925	0.0	0.0	0.0	0.0
34	1.0	0.0	0.0	0.0	0.0
35	1.075	0.0	0.0	0.0	0.0
36	1.15	0.0	0.0	0.0	0.0
37	1.175	0.0	0.0	0.0	0.0
38	1.2	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTGCGC	20	9.270304E-5	65.28302	44
GTAGTGC	25	3.2316475E-6	65.28302	44
CGTAGTG	25	1.38065825E-5	51.641792	43
CCGTAGT	25	2.3376924E-5	47.397263	42
ACCGTAG	25	2.5408139E-5	46.75676	41
ATCGCTG	20	5.099711E-4	46.75676	41
CATCGCT	25	2.7583394E-5	46.133335	40
ACCGGTT	20	7.566787E-4	43.25	24
TTCATTA	25	4.091041E-5	43.25	15
GGAGACA	25	4.091041E-5	43.25	5
ACACGGT	25	4.091041E-5	43.25	9
AACGCAT	25	4.091041E-5	43.25	36
AGCTCAA	20	7.566787E-4	43.25	31
ACGCATC	25	4.091041E-5	43.25	37
GTACCGG	20	7.566787E-4	43.25	22
TAGCTCA	20	7.566787E-4	43.25	30
GTTCATT	25	4.091041E-5	43.25	14
GGTTCAT	25	4.091041E-5	43.25	13
TCAACGC	20	7.566787E-4	43.25	34
AAGGGAG	60	0.0	43.25	2
>>END_MODULE
Read 362135 spots for SRR23332720.sra
Written 362135 spots for SRR23332720.sra
Read 362135 spots for SRR23332720.sra
Written 362135 spots for SRR23332720.sra
Read 362135 spots for SRR23332720.sra
Written 362135 spots for SRR23332720.sra
Read 362135 spots for SRR23332720.sra
Written 362135 spots for SRR23332720.sra
Read 362135 spots for SRR23332720.sra
Written 362135 spots for SRR23332720.sra
Read 362142 spots for SRR23332720.sra
Written 362142 spots for SRR23332720.sra
Read 362135 spots for SRR23332720.sra
Written 362135 spots for SRR23332720.sra
Read 362135 spots for SRR23332720.sra
Written 362135 spots for SRR23332720.sra
SRR ids: ['SRR23332720.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_9er18f5n
SRR23332720.sra spots: 2897087
blocks: [[1, 362135], [362136, 724270], [724271, 1086405], [1086406, 1448540], [1448541, 1810675], [1810676, 2172810], [2172811, 2534945], [2534946, 2897087]]
SRR23332720 file size 351480
SRR23332720 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR23332720 SRR23332720_1.fastq SRR23332720_2.fastq
Input file:	SRR23332720_1.fastq
Paired file:	SRR23332720_2.fastq
trimmed:	SRR23332720-trimmed-pair1.fastq, SRR23332720-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 03:26:57 2026 >> started

Wed Mar 25 03:26:58 2026 >> done (1.724s)
2897087 read pairs processed; of these:
    604 ( 0.02%) short read pairs filtered out after trimming by size control
     46 ( 0.00%) empty read pairs filtered out after trimming by size control
2896437 (99.98%) read pairs available; of these:
  19656 ( 0.68%) trimmed read pairs available after processing
2876781 (99.32%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    161	  0.01%
 19	    280	  0.01%
 20	    329	  0.01%
 21	    266	  0.01%
 22	    324	  0.01%
 23	    344	  0.01%
 24	    481	  0.02%
 25	    633	  0.02%
 26	    458	  0.02%
 27	    485	  0.02%
 28	    347	  0.01%
 29	    899	  0.03%
 30	    594	  0.02%
 31	    715	  0.02%
 32	    724	  0.02%
 33	    854	  0.03%
 34	   1194	  0.04%
 35	   1112	  0.04%
 36	    951	  0.03%
 37	    907	  0.03%
 38	   1369	  0.05%
 39	   1662	  0.06%
 40	   2489	  0.09%
 41	   3192	  0.11%
 42	 132820	  4.59%
 43	  63974	  2.21%
 44	 303006	 10.46%
 45	2375867	 82.03%
2896437 reads passed initial QC


criterion=sequence-density
sequence-density=2.23
sequence-density-rank=1
fanout-score=2.01
fanout-score-rank=21
prefix-density=2.25
prefix-fanout=2.0
sequence=GTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAAT


criterion=fanout-score
sequence-density=0.05
sequence-density-rank=35
fanout-score=5.62
fanout-score-rank=1
prefix-density=0.26
prefix-fanout=1.0
sequence=TCCACTCCGGTCCTCTCGTACTAGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTA


criterion=sequence-density
sequence-density=17.84
sequence-density-rank=1
fanout-score=23.35
fanout-score-rank=4
prefix-density=13.52
prefix-fanout=23.3
sequence=GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGT


criterion=fanout-score
sequence-density=0.50
sequence-density-rank=10
fanout-score=260.86
fanout-score-rank=1
prefix-density=11.01
prefix-fanout=11.8
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTAGGTCCTCT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAAT -y GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGT -o SRR23332720 SRR23332720_1.fastq SRR23332720_2.fastq
Input file:	SRR23332720_1.fastq
Paired file:	SRR23332720_2.fastq
trimmed:	SRR23332720-trimmed-pair1.fastq, SRR23332720-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAA
-- paired 3' end adapter sequence (-y):	GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 03:27:05 2026 >> started

Wed Mar 25 03:27:07 2026 >> done (1.660s)
2369812 read pairs processed; of these:
  24454 ( 1.03%) short read pairs filtered out after trimming by size control
   3431 ( 0.14%) empty read pairs filtered out after trimming by size control
2341927 (98.82%) read pairs available; of these:
    862 ( 0.04%) trimmed read pairs available after processing
2341065 (99.96%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    132	  0.01%
 19	    223	  0.01%
 20	    271	  0.01%
 21	    215	  0.01%
 22	    271	  0.01%
 23	    282	  0.01%
 24	    396	  0.02%
 25	    512	  0.02%
 26	    368	  0.02%
 27	    395	  0.02%
 28	    290	  0.01%
 29	    742	  0.03%
 30	    495	  0.02%
 31	    591	  0.03%
 32	    616	  0.03%
 33	    707	  0.03%
 34	    978	  0.04%
 35	    886	  0.04%
 36	    794	  0.03%
 37	    743	  0.03%
 38	   1124	  0.05%
 39	   1381	  0.06%
 40	   2016	  0.09%
 41	   2580	  0.11%
 42	 108439	  4.63%
 43	  52144	  2.23%
 44	 232706	  9.94%
 45	1931630	 82.48%


criterion=sequence-density
sequence-density=2.10
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=22
prefix-density=2.14
prefix-fanout=2.0
sequence=GTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAAT


criterion=fanout-score
sequence-density=0.05
sequence-density-rank=36
fanout-score=5.61
fanout-score-rank=1
prefix-density=0.26
prefix-fanout=1.0
sequence=TCCACTCCGGTCCTCTCGTACTAGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTA


criterion=sequence-density
sequence-density=17.13
sequence-density-rank=1
fanout-score=23.64
fanout-score-rank=3
prefix-density=13.42
prefix-fanout=23.6
sequence=GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGT


criterion=fanout-score
sequence-density=0.50
sequence-density-rank=10
fanout-score=257.37
fanout-score-rank=1
prefix-density=11.12
prefix-fanout=11.7
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTAGGTCCTCT
SRR23332720 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 25 03:27:25
                             Started mapping on |	Mar 25 03:27:25
                                    Finished on |	Mar 25 03:29:10
       Mapping speed, Million of reads per hour |	98.35

                          Number of input reads |	2868552
                      Average input read length |	90
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1411915
                        Uniquely mapped reads % |	49.22%
                          Average mapped length |	87.76
                       Number of splices: Total |	100640
            Number of splices: Annotated (sjdb) |	417
                       Number of splices: GT/AG |	92537
                       Number of splices: GC/AG |	2069
                       Number of splices: AT/AC |	244
               Number of splices: Non-canonical |	5790
                      Mismatch rate per base, % |	0.30%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.41
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	661526
             % of reads mapped to multiple loci |	23.06%
        Number of reads mapped to too many loci |	600857
             % of reads mapped to too many loci |	20.95%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.40%
                     % of reads unmapped: other |	0.37%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	795112	795112	795112
N_multimapping	661526	661526	661526
N_noFeature	31881	1265674	96057
N_ambiguous	182101	27417	77693
UnstrandedReadsAssigned:1197933 PositiveStrandReadsAssigned:118824 NegativeStrandReadsAssigned:1238165
Dataset is classified negative stranded
MeadianReadLen=41 20thPercentileLength=41 echo kmer=37
SRR23332720 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR23332720-trimmed-pair1.fastq
                             SRR23332720-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,868,552 reads, 667,636 reads pseudoaligned
[quant] estimated average fragment length: 209.309
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 159 rounds

  4323 SRR23332720.ke.tsv
  4497 SRR23332720.se.tsv
  8820 total
==> SRR23332720.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	12.8377	40	1225.73
AAC73113	2463	2254.69	97	16.9242
AAC73114	933	724.691	51	27.6848
AAC73115	1287	1078.69	84	30.6342
AAC73116	297	116.121	1	3.38776
AAC73117	777	568.877	36	24.8948
AAC73118	1431	1222.69	40	12.8697
AAC73119	954	745.691	925	487.986
AAC73120	588	381.243	71	73.2624

==> SRR23332720.se.tsv <==
b0001	72
b0002	95
b0003	47
b0004	79
b0005	1
b0006	41
b0007	38
b0008	928
b0009	83
b0010	9
SRR23332720 completed mapping pipeline successfully
