Starting /dee2/code/volunteer_pipeline.sh SRR23332711
    current disk space = 1458101200
    free memory = 58582136 
SRR23332711 SRAfilesize
e59d96400fbb72c978191056cfe83927  SRR23332711.sra
SRR23332711.sra file validated
SRR23332711 is paired end
SRR23332711 is conventional basespace
SRR23332711 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332711_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.46	37.0	37.0	37.0	37.0	37.0
2	36.379	37.0	37.0	37.0	37.0	37.0
3	36.395	37.0	37.0	37.0	37.0	37.0
4	36.2895	37.0	37.0	37.0	37.0	37.0
5	36.3095	37.0	37.0	37.0	37.0	37.0
6	36.347	37.0	37.0	37.0	37.0	37.0
7	36.274	37.0	37.0	37.0	37.0	37.0
8	36.24	37.0	37.0	37.0	37.0	37.0
9	36.3935	37.0	37.0	37.0	37.0	37.0
10	36.347	37.0	37.0	37.0	37.0	37.0
11	36.448	37.0	37.0	37.0	37.0	37.0
12	36.3805	37.0	37.0	37.0	37.0	37.0
13	36.4235	37.0	37.0	37.0	37.0	37.0
14	36.3045	37.0	37.0	37.0	37.0	37.0
15	36.447	37.0	37.0	37.0	37.0	37.0
16	36.4905	37.0	37.0	37.0	37.0	37.0
17	36.367	37.0	37.0	37.0	37.0	37.0
18	36.4015	37.0	37.0	37.0	37.0	37.0
19	36.3295	37.0	37.0	37.0	37.0	37.0
20	36.3935	37.0	37.0	37.0	37.0	37.0
21	36.389	37.0	37.0	37.0	37.0	37.0
22	36.313	37.0	37.0	37.0	37.0	37.0
23	36.276	37.0	37.0	37.0	37.0	37.0
24	36.347	37.0	37.0	37.0	37.0	37.0
25	36.341	37.0	37.0	37.0	37.0	37.0
26	36.1685	37.0	37.0	37.0	37.0	37.0
27	36.372	37.0	37.0	37.0	37.0	37.0
28	36.4495	37.0	37.0	37.0	37.0	37.0
29	36.366	37.0	37.0	37.0	37.0	37.0
30	36.313	37.0	37.0	37.0	37.0	37.0
31	36.392	37.0	37.0	37.0	37.0	37.0
32	36.3875	37.0	37.0	37.0	37.0	37.0
33	36.446	37.0	37.0	37.0	37.0	37.0
34	36.2875	37.0	37.0	37.0	37.0	37.0
35	36.309	37.0	37.0	37.0	37.0	37.0
36	36.1785	37.0	37.0	37.0	37.0	37.0
37	36.2245	37.0	37.0	37.0	37.0	37.0
38	36.1615	37.0	37.0	37.0	37.0	37.0
39	36.205	37.0	37.0	37.0	37.0	37.0
40	36.2225	37.0	37.0	37.0	37.0	37.0
41	36.166	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	3.0
26	3.0
27	16.0
28	13.0
29	26.0
30	30.0
31	42.0
32	43.0
33	78.0
34	122.0
35	229.0
36	1319.0
37	2075.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.799999999999997	20.150000000000002	47.925000000000004	8.125
2	28.599999999999998	18.099999999999998	32.05	21.25
3	29.125	21.15	29.775000000000002	19.950000000000003
4	21.6	22.7	34.775	20.925
5	31.025000000000002	24.25	22.25	22.475
6	32.550000000000004	23.474999999999998	23.35	20.625
7	23.525	33.6	18.475	24.4
8	34.4	19.3	21.4	24.9
9	25.45	16.35	21.325	36.875
10	26.125	16.275000000000002	30.5	27.1
11	34.449999999999996	17.0	20.474999999999998	28.075
12	36.05	16.825000000000003	22.075	25.05
13	26.200000000000003	19.075	20.125	34.599999999999994
14	37.125	20.175	21.9	20.8
15	35.0	21.3	22.45	21.25
16	38.175	19.55	23.075000000000003	19.2
17	25.074999999999996	32.875	23.65	18.4
18	33.5	22.15	24.5	19.85
19	26.924999999999997	21.275	32.775	19.025
20	26.825	20.075000000000003	31.125000000000004	21.975
21	35.625	22.3	19.2	22.875
22	24.75	31.525	21.2	22.525000000000002
23	25.05	30.575000000000003	20.200000000000003	24.175
24	24.95	21.475	18.925	34.65
25	28.65	18.925	21.2	31.225
26	24.075	19.175	23.375	33.375
27	37.375	20.075000000000003	20.474999999999998	22.075
28	24.525	18.925	22.325	34.225
29	35.75	19.0	21.375	23.875
30	22.7	20.075000000000003	33.35	23.875
31	25.424999999999997	21.275	22.675	30.625000000000004
32	25.4	22.075	22.525000000000002	30.0
33	33.85	22.3	22.475	21.375
34	22.175	33.300000000000004	22.05	22.475
35	22.725	32.125	22.375	22.775000000000002
36	21.45	33.650000000000006	21.9	23.0
37	22.575	22.75	30.8	23.875
38	23.625	21.825	28.799999999999997	25.75
39	23.5	21.575	21.875	33.050000000000004
40	21.375	24.8	20.8	33.025
41	22.425	22.125	30.5	24.95
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	1.0
20	1.0
21	1.0
22	1.0
23	1.5
24	2.0
25	2.0
26	3.5
27	5.0
28	8.5
29	12.0
30	14.5
31	17.0
32	17.0
33	21.0
34	25.0
35	29.5
36	34.0
37	34.0
38	52.0
39	70.0
40	124.0
41	178.0
42	178.0
43	233.0
44	288.0
45	292.5
46	297.0
47	297.0
48	379.5
49	462.0
50	474.0
51	486.0
52	519.0
53	552.0
54	552.0
55	457.0
56	362.0
57	329.5
58	297.0
59	297.0
60	503.0
61	709.0
62	405.5
63	102.0
64	102.0
65	77.0
66	52.0
67	40.0
68	28.0
69	28.0
70	21.0
71	14.0
72	10.0
73	6.0
74	3.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.525000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.03588210166595	44.5
2	15.591627509611278	18.25
3	4.058094831268689	7.124999999999999
4	1.3242204186245194	3.1
5	0.9824861170439982	2.875
6	0.46988466467321655	1.6500000000000001
7	0.299017513882956	1.225
8	0.299017513882956	1.4000000000000001
9	0.08543357539513029	0.44999999999999996
>10	0.7689021785561726	7.249999999999999
>50	0.042716787697565144	1.55
>100	0.042716787697565144	10.625
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCT	425	10.625	No Hit
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTAC	62	1.55	No Hit
AAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGAG	39	0.975	No Hit
GGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTA	30	0.75	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCC	26	0.65	No Hit
GGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACAT	24	0.6	No Hit
AGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCCA	16	0.4	No Hit
GGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAAC	14	0.35000000000000003	No Hit
AAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTT	14	0.35000000000000003	No Hit
GCAGGGCTTCCACGCGATCTCTTTTGGGTTTGACCTCTCTT	13	0.325	No Hit
AAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTT	13	0.325	No Hit
AAAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGA	13	0.325	No Hit
AAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTC	12	0.3	No Hit
GCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACTAC	12	0.3	No Hit
GCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACA	12	0.3	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCC	12	0.3	No Hit
GGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTC	10	0.25	No Hit
TTTCGTGCTTAGATGAGTTCTCCCTGACTCCTTGAGAGTCC	10	0.25	No Hit
TCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGT	10	0.25	No Hit
TGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	10	0.25	No Hit
TGCAGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATC	9	0.22499999999999998	No Hit
TTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCAC	9	0.22499999999999998	No Hit
GGTTAAGCTACCTACTTTGTGATTCATGACTGGGGTGAAGT	8	0.2	No Hit
TTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGA	8	0.2	No Hit
TACCTACTTCTTTTGCAACCCACTTTGTGATTCATGACTGG	8	0.2	No Hit
TCTCGTACTAGGAGTGGACGCATCACTGGTGTTCGGGTTGT	8	0.2	No Hit
TCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGACT	8	0.2	No Hit
TTATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGAA	8	0.2	No Hit
TTCTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAG	8	0.2	No Hit
GGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAACC	7	0.17500000000000002	No Hit
GTGTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACC	7	0.17500000000000002	No Hit
GGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAA	7	0.17500000000000002	No Hit
TGTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCT	7	0.17500000000000002	No Hit
TGTGTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCAC	7	0.17500000000000002	No Hit
TGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACA	7	0.17500000000000002	No Hit
ATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA	7	0.17500000000000002	No Hit
TGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCACTA	6	0.15	No Hit
TTTGGGTTTGACCTCTCTTGATCCCCGTCCTAAGAGCGGAG	6	0.15	No Hit
TATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAG	6	0.15	No Hit
AACAAACTAGCCTGATTAAGTTTTAACGCTTCAACCCCAGG	6	0.15	No Hit
TCTTTACATTCGCTTGCCAGCTGCGGACGGACACGCCACTA	6	0.15	No Hit
ACTCTCAAGGAACGTTGAAGACGACGACGTTGATAGGCCGG	6	0.15	No Hit
GAGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGG	6	0.15	No Hit
TTTATGAGGTCCGGATTGGAGTCTGCAACTCGACTCCATGA	6	0.15	No Hit
TTTGAGTTCCCGGGCCTTGTACACACCGCCCGTCACACCAT	6	0.15	No Hit
TCTCGCGAGGTCGGAATCGCTAGTAATCGTGGATCAGAATG	6	0.15	No Hit
AAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAG	6	0.15	No Hit
TTCCGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTT	5	0.125	No Hit
TAACAAACTAGCCTGATTAAGTTTTAACGCTTCAACCCCAG	5	0.125	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAAC	5	0.125	No Hit
GGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	5	0.125	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACGAGAGGACCGGGA	5	0.125	No Hit
TTCCACGCGATCTCTTTTGGGTTTGACCTCTCTTGATCCCC	5	0.125	No Hit
TATCCGTTGAGCGATGCGTTGAGCTAACCGGTACTAATGAA	5	0.125	No Hit
TTTCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGA	5	0.125	No Hit
AAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGT	5	0.125	No Hit
CTTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGTC	5	0.125	No Hit
ATAAAAAGCCGCTCGGGAGTAAGCGAGCGGTGGTCAAATAA	5	0.125	No Hit
AGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAA	5	0.125	No Hit
TTTTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGA	5	0.125	No Hit
TTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGTCGTA	5	0.125	No Hit
TTTCGTGCTTAGATGAGTTCTCCCTGACCCTTTAAGGGTCC	5	0.125	No Hit
TTTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAG	5	0.125	No Hit
TCCTACATCCTCGGTACTACATGCTTAGTCAGTCTTTACAT	5	0.125	No Hit
TTTACATTCGCTTGCCAGCTGCGGACGGACACGCCACTAAC	5	0.125	No Hit
ATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCA	5	0.125	No Hit
GTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTC	5	0.125	No Hit
TTGAGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATG	5	0.125	No Hit
TCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGACTCC	5	0.125	No Hit
TATCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.025	0.0	0.0	0.0	0.0
9	0.025	0.0	0.0	0.0	0.0
10	0.025	0.0	0.0	0.0	0.0
11	0.025	0.0	0.0	0.0	0.0
12	0.025	0.0	0.0	0.0	0.0
13	0.025	0.0	0.0	0.0	0.0
14	0.05	0.0	0.0	0.0	0.0
15	0.05	0.0	0.0	0.0	0.0
16	0.05	0.0	0.0	0.0	0.0
17	0.1	0.0	0.0	0.0	0.0
18	0.1	0.0	0.0	0.0	0.0
19	0.125	0.0	0.0	0.0	0.0
20	0.15	0.0	0.0	0.0	0.0
21	0.275	0.0	0.0	0.0	0.0
22	0.4	0.0	0.0	0.0	0.0
23	0.4	0.0	0.0	0.0	0.0
24	0.55	0.0	0.0	0.0	0.0
25	0.575	0.0	0.0	0.0	0.0
26	0.675	0.0	0.0	0.0	0.0
27	0.7	0.0	0.0	0.0	0.0
28	0.7	0.0	0.0	0.0	0.0
29	0.75	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTGGA	70	0.0	35.000004	1
TGGAGCT	75	0.0	32.666668	4
GGTGGAG	75	0.0	32.666668	2
GTGGAGC	75	0.0	32.666668	3
GCTGGCG	80	0.0	30.625	8
AGCTGGC	80	0.0	30.625	7
GGAGCTG	80	0.0	30.625	5
GCGGGAG	85	0.0	28.823528	12
GTTGAAC	85	0.0	28.823528	18
GAAATTC	85	0.0	28.823528	33
AAATTCC	85	0.0	28.823528	34
AATTCCT	85	0.0	28.823528	35
GAGCTGG	85	0.0	28.823528	6
AACCCGC	85	0.0	28.823528	22
GGGAGTT	85	0.0	28.823528	14
GAGTTGA	85	0.0	28.823528	16
CTGGCGG	85	0.0	28.823528	9
GAACCCG	85	0.0	28.823528	21
CGGGAGT	85	0.0	28.823528	13
AGTTGAA	85	0.0	28.823528	17
>>END_MODULE
SRR23332711 read2 length is 42-50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332711_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	42-50
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.81775	11.0	11.0	37.0	11.0	37.0
2	23.4445	25.0	11.0	37.0	11.0	37.0
3	25.6205	25.0	11.0	37.0	11.0	37.0
4	35.969	37.0	37.0	37.0	37.0	37.0
5	36.107	37.0	37.0	37.0	37.0	37.0
6	36.086	37.0	37.0	37.0	37.0	37.0
7	35.853	37.0	37.0	37.0	37.0	37.0
8	36.038	37.0	37.0	37.0	37.0	37.0
9	35.894	37.0	37.0	37.0	37.0	37.0
10	36.036	37.0	37.0	37.0	37.0	37.0
11	36.014	37.0	37.0	37.0	37.0	37.0
12	36.0245	37.0	37.0	37.0	37.0	37.0
13	36.0105	37.0	37.0	37.0	37.0	37.0
14	36.1195	37.0	37.0	37.0	37.0	37.0
15	35.8695	37.0	37.0	37.0	37.0	37.0
16	36.179	37.0	37.0	37.0	37.0	37.0
17	36.0885	37.0	37.0	37.0	37.0	37.0
18	36.225	37.0	37.0	37.0	37.0	37.0
19	36.266	37.0	37.0	37.0	37.0	37.0
20	36.1765	37.0	37.0	37.0	37.0	37.0
21	36.1015	37.0	37.0	37.0	37.0	37.0
22	36.13575	37.0	37.0	37.0	37.0	37.0
23	36.144	37.0	37.0	37.0	37.0	37.0
24	36.20975	37.0	37.0	37.0	37.0	37.0
25	36.176	37.0	37.0	37.0	37.0	37.0
26	36.0625	37.0	37.0	37.0	37.0	37.0
27	36.06325	37.0	37.0	37.0	37.0	37.0
28	36.0995	37.0	37.0	37.0	37.0	37.0
29	36.12575	37.0	37.0	37.0	37.0	37.0
30	36.19825	37.0	37.0	37.0	37.0	37.0
31	36.05475	37.0	37.0	37.0	37.0	37.0
32	36.018	37.0	37.0	37.0	37.0	37.0
33	36.09225	37.0	37.0	37.0	37.0	37.0
34	36.39225	37.0	37.0	37.0	37.0	37.0
35	36.16625	37.0	37.0	37.0	37.0	37.0
36	36.31225	37.0	37.0	37.0	37.0	37.0
37	36.1895	37.0	37.0	37.0	37.0	37.0
38	36.224	37.0	37.0	37.0	37.0	37.0
39	36.1245	37.0	37.0	37.0	37.0	37.0
40	36.216	37.0	37.0	37.0	37.0	37.0
41	36.0145	37.0	37.0	37.0	37.0	37.0
42	36.16425	37.0	37.0	37.0	37.0	37.0
43	35.88157235853781	37.0	37.0	37.0	37.0	37.0
44	36.137791286727456	37.0	37.0	37.0	37.0	37.0
45	35.879695155144255	37.0	37.0	37.0	37.0	37.0
46	36.00303113805456	37.0	37.0	37.0	37.0	37.0
47	36.02742437093582	37.0	37.0	37.0	37.0	37.0
48	36.06576680068926	37.0	37.0	37.0	37.0	37.0
49	36.178676230844474	37.0	37.0	37.0	37.0	37.0
50	36.05320813771518	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	1.0
19	1.0
20	1.0
21	1.0
22	8.0
23	8.0
24	11.0
25	6.0
26	14.0
27	17.0
28	19.0
29	29.0
30	41.0
31	58.0
32	83.0
33	133.0
34	388.0
35	1695.0
36	1215.0
37	269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	39.35983995999	29.80745186296574	30.83270817704426
2	39.300000000000004	33.15	12.35	15.2
3	32.225	37.675	14.05	16.05
4	78.4	7.75	5.025	8.825
5	65.325	17.175	10.925	6.575
6	57.025000000000006	20.175	9.225	13.575000000000001
7	39.775	39.725	8.95	11.55
8	44.625	21.525	12.4	21.45
9	21.275	39.574999999999996	26.775	12.375
10	40.675	24.65	17.150000000000002	17.525
11	29.2	39.2	16.35	15.25
12	22.425	27.825	25.95	23.799999999999997
13	37.775	14.05	28.15	20.025000000000002
14	35.375	16.225	24.55	23.849999999999998
15	28.1	19.2	41.975	10.725
16	44.975	21.55	22.45	11.025
17	25.95	32.275	18.825	22.95
18	15.9	39.324999999999996	27.200000000000003	17.575
19	18.8	18.575	34.075	28.549999999999997
20	14.399999999999999	22.925	30.525000000000002	32.15
21	19.025	42.675000000000004	13.8	24.5
22	38.33458364591148	30.282570642660666	14.428607151787947	16.954238559639908
23	22.575	30.075000000000003	20.5	26.85
24	22.305576394098527	22.780695173793447	14.128532133033259	40.78519629907477
25	38.35	18.075	13.450000000000001	30.125
26	34.14207103551776	19.884942471235618	13.206603301650826	32.7663831915958
27	38.63465866466617	24.50612653163291	19.679919979995	17.179294823705927
28	34.19209604802401	24.037018509254626	26.93846923461731	14.832416208104052
29	23.63090772693173	14.778694673668417	45.361340335083774	16.22905726431608
30	19.014260695521642	16.312234175631723	51.01325994495872	13.660245183887914
31	16.9792448112028	24.20605151287822	38.759689922480625	20.05501375343836
32	32.69134567283642	13.781890945472735	20.060030015007506	33.46673336668334
33	23.680920230057513	13.87846961740435	14.228557139284822	48.21205301325332
34	25.35633908477119	18.404601150287572	23.055763940985248	33.18329582395599
35	11.902975743935984	35.73393348337085	23.10577644411103	29.257314328582147
36	13.578394598649663	38.60965241310328	28.982245561390346	18.829707426856714
37	16.875	45.675	12.1	25.35
38	23.775	18.35	10.7	47.175
39	35.825	15.875	12.1	36.199999999999996
40	22.325	20.65	20.4	36.625
41	17.95	38.45	27.925	15.675
42	21.705426356589147	27.33183295823956	29.43235808952238	21.530382595648913
43	31.43000250438267	24.292511895817682	15.852742299023289	28.424743300776356
44	35.48632218844985	16.134751773049647	18.9209726443769	29.45795339412361
45	17.446924333151877	16.46706586826347	32.96135002721829	33.12465977136636
46	29.62248553320474	20.088178561587213	31.71672637090108	18.57260953430697
47	28.498727735368956	16.878710771840545	27.085100367543113	27.537461125247386
48	28.957196207986215	24.102269462798045	24.59063487503591	22.349899454179834
49	30.658838878016958	11.839530332681017	40.21526418786693	17.286366601435095
50	28.677621283255085	14.006259780907667	28.09076682316119	29.225352112676056
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	0.5
24	0.0
25	0.5
26	1.5
27	3.0
28	4.0
29	8.5
30	14.5
31	18.0
32	23.5
33	31.5
34	38.5
35	42.0
36	40.5
37	44.0
38	51.0
39	59.5
40	74.0
41	94.5
42	107.0
43	110.5
44	115.5
45	117.0
46	119.5
47	157.5
48	199.5
49	209.5
50	230.5
51	384.0
52	530.5
53	709.0
54	899.0
55	898.5
56	819.0
57	656.0
58	509.0
59	332.0
60	181.0
61	120.0
62	69.5
63	49.0
64	30.0
65	24.0
66	18.5
67	10.0
68	5.0
69	4.5
70	3.5
71	2.0
72	0.5
73	0.5
74	1.0
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.5
82	1.0
83	0.5
84	0.0
85	1.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.025
23	0.0
24	0.025
25	0.0
26	0.05
27	0.025
28	0.05
29	0.025
30	0.075
31	0.025
32	0.05
33	0.025
34	0.025
35	0.025
36	0.025
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.025
43	0.025037556334501748
44	0.0
45	0.0
46	0.0
47	0.0
48	0.02871912693854107
49	0.032605151613955004
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
42	6.0
43	46.0
44	274.0
45	45.0
46	92.0
47	55.0
48	415.0
49	511.0
50	2556.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.95
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.03796376186368	48.699999999999996
2	6.125970664365832	7.1
3	2.6747195858498705	4.65
4	0.992234685073339	2.3
5	1.553062985332183	4.5
6	0.7765314926660914	2.7
7	0.9490940465918896	3.85
8	0.6471095772217429	3.0
9	0.5608283002588439	2.9250000000000003
>10	1.5099223468507335	14.524999999999999
>50	0.1725625539257981	5.75
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	64	1.6	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	61	1.525	No Hit
AAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	53	1.325	No Hit
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	52	1.3	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	50	1.25	No Hit
AAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	32	0.8	No Hit
CGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	31	0.775	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	30	0.75	No Hit
TGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	29	0.7250000000000001	No Hit
CAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	26	0.65	No Hit
CTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	25	0.625	No Hit
TAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	25	0.625	No Hit
AACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	17	0.42500000000000004	No Hit
CGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	17	0.42500000000000004	No Hit
CAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	16	0.4	No Hit
AAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
TGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	15	0.375	No Hit
TAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	15	0.375	No Hit
CGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
TGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	14	0.35000000000000003	No Hit
CAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	14	0.35000000000000003	No Hit
AATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
TGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	13	0.325	No Hit
AGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
CGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
CCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	11	0.27499999999999997	No Hit
CGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
CGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
CCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
ACAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTA	10	0.25	No Hit
AGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	10	0.25	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGT	10	0.25	No Hit
ACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
TTTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
AACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	10	0.25	No Hit
AGACACGGTACTAATGAACCGTGAGGCTTAACCTACAACTCGCA	9	0.22499999999999998	No Hit
AAAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	9	0.22499999999999998	No Hit
CACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TGTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
CTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCT	9	0.22499999999999998	No Hit
ACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
TTGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
CCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
TAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	9	0.22499999999999998	No Hit
AGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	9	0.22499999999999998	No Hit
TCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	9	0.22499999999999998	No Hit
CGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
TCGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
CGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
TGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CGCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
CCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
AGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
AATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTCAAC	8	0.2	No Hit
CACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TGTGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
TCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AATGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	7	0.17500000000000002	No Hit
CGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TCCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
AAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
AAAGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCT	7	0.17500000000000002	No Hit
TGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CGTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
AGACACGGTACTAATGAACCGTGAGGCTTAACCTTACAACTCGC	7	0.17500000000000002	No Hit
TATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
TACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
CGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
CCCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TTGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TTCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AAGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
ACCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
CAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TGCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
ATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TAGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
ACCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TGAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	6	0.15	No Hit
TGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TTTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AAAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAT	5	0.125	No Hit
ATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	5	0.125	No Hit
TGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
CGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	5	0.125	No Hit
AAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
AGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTA	5	0.125	No Hit
AAAGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	5	0.125	No Hit
CGGGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CCTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CGCGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
TATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AGAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TATGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CCAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
AGTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
AACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TAGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
CATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
ACAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
TCAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCT	5	0.125	No Hit
ATAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGC	5	0.125	No Hit
CGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
CTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.025	0.0	0.0	0.0	0.0
18	0.025	0.0	0.0	0.0	0.0
19	0.025	0.0	0.0	0.0	0.0
20	0.05	0.0	0.0	0.0	0.0
21	0.075	0.0	0.0	0.0	0.0
22	0.075	0.0	0.0	0.0	0.0
23	0.175	0.0	0.0	0.0	0.0
24	0.2	0.0	0.0	0.0	0.0
25	0.2	0.0	0.0	0.0	0.0
26	0.25	0.0	0.0	0.0	0.0
27	0.35	0.0	0.0	0.0	0.0
28	0.4	0.0	0.0	0.0	0.0
29	0.45	0.0	0.0	0.0	0.0
30	0.5	0.0	0.0	0.0	0.0
31	0.55	0.0	0.0	0.0	0.0
32	0.55	0.0	0.0	0.0	0.0
33	0.575	0.0	0.0	0.0	0.0
34	0.65	0.0	0.0	0.0	0.0
35	0.7	0.0	0.0	0.0	0.0
36	0.7	0.0	0.0	0.0	0.0
37	0.725	0.0	0.0	0.0	0.0
38	0.75	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCATG	65	0.0	56.80328	44
GCTGCGC	25	5.701292E-4	45.442623	44
GGTGCAT	75	0.0	44.161766	43
AGGGGCT	30	2.1752257E-6	43.3125	3
AAGGGAG	20	7.513131E-4	43.3125	2
AAAGGGG	20	7.513131E-4	43.3125	1
AGGTGCA	75	0.0	41.708336	42
CGCTGCG	25	9.906219E-4	40.764706	43
AAGGTGC	75	0.0	40.58108	41
CAAGGTG	75	0.0	40.04	40
CCAAGGT	75	0.0	39.51316	39
GGGAGAG	45	1.731678E-8	38.5	4
TCGCTGC	25	0.0013232694	38.5	42
GAAACCC	75	0.0	37.537502	34
TCTGGAT	75	0.0	37.537502	13
GACGGGA	75	0.0	37.537502	22
GGATTTG	75	0.0	37.537502	26
TGCGAAA	75	0.0	37.537502	31
GCGAAAC	75	0.0	37.537502	32
CTGGATT	75	0.0	37.537502	14
>>END_MODULE
Read 26613 spots for SRR23332711.sra
Written 26613 spots for SRR23332711.sra
Read 26608 spots for SRR23332711.sra
Written 26608 spots for SRR23332711.sra
Read 26608 spots for SRR23332711.sra
Written 26608 spots for SRR23332711.sra
Read 26608 spots for SRR23332711.sra
Written 26608 spots for SRR23332711.sra
Read 26608 spots for SRR23332711.sra
Written 26608 spots for SRR23332711.sra
Read 26608 spots for SRR23332711.sra
Written 26608 spots for SRR23332711.sra
Read 26608 spots for SRR23332711.sra
Written 26608 spots for SRR23332711.sra
Read 26608 spots for SRR23332711.sra
Written 26608 spots for SRR23332711.sra
SRR ids: ['SRR23332711.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_gkk5460q
SRR23332711.sra spots: 212869
blocks: [[1, 26608], [26609, 53216], [53217, 79824], [79825, 106432], [106433, 133040], [133041, 159648], [159649, 186256], [186257, 212869]]
SRR23332711 file size 25356
SRR23332711 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR23332711 SRR23332711_1.fastq SRR23332711_2.fastq
Input file:	SRR23332711_1.fastq
Paired file:	SRR23332711_2.fastq
trimmed:	SRR23332711-trimmed-pair1.fastq, SRR23332711-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 03:53:41 2026 >> started

Wed Mar 25 03:53:42 2026 >> done (0.217s)
212869 read pairs processed; of these:
    40 ( 0.02%) short read pairs filtered out after trimming by size control
     0 ( 0.00%) empty read pairs filtered out after trimming by size control
212829 (99.98%) read pairs available; of these:
  1595 ( 0.75%) trimmed read pairs available after processing
211234 (99.25%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    16	  0.01%
 19	    14	  0.01%
 20	    57	  0.03%
 21	    33	  0.02%
 22	    27	  0.01%
 23	    22	  0.01%
 24	    34	  0.02%
 25	    48	  0.02%
 26	    32	  0.02%
 27	    23	  0.01%
 28	    27	  0.01%
 29	    39	  0.02%
 30	    39	  0.02%
 31	    53	  0.02%
 32	    37	  0.02%
 33	    67	  0.03%
 34	   108	  0.05%
 35	   123	  0.06%
 36	   113	  0.05%
 37	    82	  0.04%
 38	    89	  0.04%
 39	   108	  0.05%
 40	   265	  0.12%
 41	   397	  0.19%
 42	 13210	  6.21%
 43	  6078	  2.86%
 44	 22489	 10.57%
 45	169199	 79.50%
212829 reads passed initial QC


criterion=sequence-density
sequence-density=1.43
sequence-density-rank=1
fanout-score=2.44
fanout-score-rank=11
prefix-density=1.42
prefix-fanout=2.4
sequence=ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG


criterion=fanout-score
sequence-density=0.12
sequence-density-rank=30
fanout-score=4.87
fanout-score-rank=1
prefix-density=0.60
prefix-fanout=1.0
sequence=GTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGACTCCTTGAGAGTCCTGAAGGAACGTTG


criterion=sequence-density
sequence-density=15.33
sequence-density-rank=1
fanout-score=22.05
fanout-score-rank=3
prefix-density=12.40
prefix-fanout=22.1
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAG


criterion=fanout-score
sequence-density=0.60
sequence-density-rank=12
fanout-score=188.93
fanout-score-rank=1
prefix-density=10.23
prefix-fanout=11.1
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTACAACTCGC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG -y GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAG -o SRR23332711 SRR23332711_1.fastq SRR23332711_2.fastq
Input file:	SRR23332711_1.fastq
Paired file:	SRR23332711_2.fastq
trimmed:	SRR23332711-trimmed-pair1.fastq, SRR23332711-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG
-- paired 3' end adapter sequence (-y):	GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 03:53:43 2026 >> started

Wed Mar 25 03:53:43 2026 >> done (0.190s)
165534 read pairs processed; of these:
  1411 ( 0.85%) short read pairs filtered out after trimming by size control
   100 ( 0.06%) empty read pairs filtered out after trimming by size control
164023 (99.09%) read pairs available; of these:
    71 ( 0.04%) trimmed read pairs available after processing
163952 (99.96%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    13	  0.01%
 19	    10	  0.01%
 20	    42	  0.03%
 21	    28	  0.02%
 22	    18	  0.01%
 23	    20	  0.01%
 24	    25	  0.02%
 25	    34	  0.02%
 26	    25	  0.02%
 27	    17	  0.01%
 28	    23	  0.01%
 29	    30	  0.02%
 30	    27	  0.02%
 31	    40	  0.02%
 32	    29	  0.02%
 33	    51	  0.03%
 34	    92	  0.06%
 35	   102	  0.06%
 36	    91	  0.06%
 37	    65	  0.04%
 38	    67	  0.04%
 39	    84	  0.05%
 40	   198	  0.12%
 41	   306	  0.19%
 42	 10225	  6.23%
 43	  4743	  2.89%
 44	 16993	 10.36%
 45	130625	 79.64%


criterion=sequence-density
sequence-density=1.40
sequence-density-rank=1
fanout-score=2.49
fanout-score-rank=9
prefix-density=1.43
prefix-fanout=2.4
sequence=ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG


criterion=fanout-score
sequence-density=0.12
sequence-density-rank=29
fanout-score=4.87
fanout-score-rank=1
prefix-density=0.60
prefix-fanout=1.0
sequence=GTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATTTAGCTACCGAGATGAGTTCTCCCTGACTCCTTGAGAGTCCTGAAGGAACGTTG


criterion=sequence-density
sequence-density=15.32
sequence-density-rank=1
fanout-score=24.60
fanout-score-rank=2
prefix-density=11.23
prefix-fanout=24.6
sequence=GGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTAC


criterion=fanout-score
sequence-density=0.61
sequence-density-rank=12
fanout-score=188.93
fanout-score-rank=1
prefix-density=10.30
prefix-fanout=11.1
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTACAACTCGC
SRR23332711 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 25 03:53:56
                             Started mapping on |	Mar 25 03:53:56
                                    Finished on |	Mar 25 03:54:40
       Mapping speed, Million of reads per hour |	17.29

                          Number of input reads |	211318
                      Average input read length |	89
                                    UNIQUE READS:
                   Uniquely mapped reads number |	109813
                        Uniquely mapped reads % |	51.97%
                          Average mapped length |	87.62
                       Number of splices: Total |	6751
            Number of splices: Annotated (sjdb) |	59
                       Number of splices: GT/AG |	6081
                       Number of splices: GC/AG |	134
                       Number of splices: AT/AC |	9
               Number of splices: Non-canonical |	527
                      Mismatch rate per base, % |	0.26%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.23
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	46802
             % of reads mapped to multiple loci |	22.15%
        Number of reads mapped to too many loci |	42167
             % of reads mapped to too many loci |	19.95%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.64%
                     % of reads unmapped: other |	0.29%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	54703	54703	54703
N_multimapping	46802	46802	46802
N_noFeature	3100	99536	7263
N_ambiguous	11414	1794	4049
UnstrandedReadsAssigned:95299 PositiveStrandReadsAssigned:8483 NegativeStrandReadsAssigned:98501
Dataset is classified negative stranded
MeadianReadLen=41 20thPercentileLength=41 echo kmer=37
SRR23332711 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR23332711-trimmed-pair1.fastq
                             SRR23332711-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 211,318 reads, 36,350 reads pseudoaligned
[quant] estimated average fragment length: 205.21
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 52 rounds

  4323 SRR23332711.ke.tsv
  4497 SRR23332711.se.tsv
  8820 total
==> SRR23332711.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	12.8833	6	2213.8
AAC73113	2463	2258.79	3	6.31335
AAC73114	933	728.79	8	52.1798
AAC73115	1287	1082.79	2	8.78012
AAC73116	297	120.774	0	0
AAC73117	777	573.019	4	33.1822
AAC73118	1431	1226.79	3	11.6243
AAC73119	954	749.79	48	304.31
AAC73120	588	385.013	4	49.3854

==> SRR23332711.se.tsv <==
b0001	14
b0002	3
b0003	8
b0004	2
b0005	0
b0006	4
b0007	3
b0008	52
b0009	5
b0010	2
SRR23332711 completed mapping pipeline successfully
