Starting /dee2/code/volunteer_pipeline.sh SRR23332710
    current disk space = 1458053180
    free memory = 58220320 
SRR23332710 SRAfilesize
c7994cb32ca441808eb7ecb0c03d3018  SRR23332710.sra
SRR23332710.sra file validated
SRR23332710 is paired end
SRR23332710 is conventional basespace
SRR23332710 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332710_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.409	37.0	37.0	37.0	37.0	37.0
2	36.475	37.0	37.0	37.0	37.0	37.0
3	36.369	37.0	37.0	37.0	37.0	37.0
4	36.33	37.0	37.0	37.0	37.0	37.0
5	36.268	37.0	37.0	37.0	37.0	37.0
6	36.2655	37.0	37.0	37.0	37.0	37.0
7	36.2445	37.0	37.0	37.0	37.0	37.0
8	36.385	37.0	37.0	37.0	37.0	37.0
9	36.4065	37.0	37.0	37.0	37.0	37.0
10	36.44	37.0	37.0	37.0	37.0	37.0
11	36.411	37.0	37.0	37.0	37.0	37.0
12	36.417	37.0	37.0	37.0	37.0	37.0
13	36.4055	37.0	37.0	37.0	37.0	37.0
14	36.398	37.0	37.0	37.0	37.0	37.0
15	36.4355	37.0	37.0	37.0	37.0	37.0
16	36.4005	37.0	37.0	37.0	37.0	37.0
17	36.245	37.0	37.0	37.0	37.0	37.0
18	36.201	37.0	37.0	37.0	37.0	37.0
19	36.0795	37.0	37.0	37.0	37.0	37.0
20	36.325	37.0	37.0	37.0	37.0	37.0
21	36.3145	37.0	37.0	37.0	37.0	37.0
22	36.2295	37.0	37.0	37.0	37.0	37.0
23	36.3355	37.0	37.0	37.0	37.0	37.0
24	36.291	37.0	37.0	37.0	37.0	37.0
25	36.426	37.0	37.0	37.0	37.0	37.0
26	36.138	37.0	37.0	37.0	37.0	37.0
27	36.3455	37.0	37.0	37.0	37.0	37.0
28	36.248	37.0	37.0	37.0	37.0	37.0
29	36.306	37.0	37.0	37.0	37.0	37.0
30	36.2265	37.0	37.0	37.0	37.0	37.0
31	36.3415	37.0	37.0	37.0	37.0	37.0
32	36.2255	37.0	37.0	37.0	37.0	37.0
33	36.4265	37.0	37.0	37.0	37.0	37.0
34	36.198	37.0	37.0	37.0	37.0	37.0
35	36.2755	37.0	37.0	37.0	37.0	37.0
36	35.973	37.0	37.0	37.0	37.0	37.0
37	36.1735	37.0	37.0	37.0	37.0	37.0
38	36.37	37.0	37.0	37.0	37.0	37.0
39	36.031	37.0	37.0	37.0	37.0	37.0
40	36.1505	37.0	37.0	37.0	37.0	37.0
41	36.1435	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	1.0
26	5.0
27	8.0
28	12.0
29	22.0
30	25.0
31	47.0
32	61.0
33	86.0
34	146.0
35	301.0
36	1325.0
37	1961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.325	22.925	48.225	7.5249999999999995
2	38.6	21.775	23.275000000000002	16.35
3	41.925000000000004	22.8	21.275	14.000000000000002
4	18.099999999999998	24.3	40.5	17.1
5	39.875	24.725	17.224999999999998	18.175
6	41.775	23.400000000000002	18.35	16.475
7	20.349999999999998	44.574999999999996	15.325	19.75
8	43.824999999999996	17.474999999999998	15.825	22.875
9	25.1	12.825000000000001	15.675	46.400000000000006
10	23.474999999999998	12.725	39.0	24.8
11	44.425	14.549999999999999	14.475	26.55
12	42.85	15.65	17.549999999999997	23.95
13	24.55	15.075	16.3	44.074999999999996
14	44.125	17.349999999999998	18.625	19.900000000000002
15	45.5	17.424999999999997	18.375	18.7
16	45.5	19.15	19.5	15.85
17	21.05	41.175	20.875	16.900000000000002
18	41.55	19.925	21.349999999999998	17.175
19	24.224999999999998	16.325	42.125	17.325
20	24.375	17.325	36.9	21.4
21	45.375	20.349999999999998	15.675	18.6
22	21.65	42.275	15.925	20.150000000000002
23	22.85	40.949999999999996	15.375	20.825
24	22.6	19.55	13.350000000000001	44.5
25	24.675	15.65	17.4	42.275
26	21.575	15.15	21.425	41.85
27	44.975	17.724999999999998	16.875	20.424999999999997
28	19.55	14.875	19.2	46.375
29	42.875	15.4	21.175	20.549999999999997
30	19.900000000000002	16.625	42.4	21.075
31	18.825	18.975	19.625	42.575
32	22.825	18.625	17.4	41.15
33	42.575	19.225	19.8	18.4
34	19.575	42.449999999999996	18.075	19.900000000000002
35	17.224999999999998	42.225	21.85	18.7
36	16.650000000000002	42.65	20.025000000000002	20.674999999999997
37	19.075	20.45	40.699999999999996	19.775000000000002
38	21.65	18.85	38.574999999999996	20.925
39	21.875	18.325	18.675	41.125
40	18.325	21.224999999999998	18.35	42.1
41	18.9	19.525000000000002	39.425	22.15
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.5
27	1.0
28	2.0
29	3.0
30	3.5
31	4.0
32	4.0
33	5.5
34	7.0
35	12.0
36	17.0
37	17.0
38	42.0
39	67.0
40	107.5
41	148.0
42	148.0
43	206.0
44	264.0
45	278.5
46	293.0
47	293.0
48	326.5
49	360.0
50	386.0
51	412.0
52	410.5
53	409.0
54	409.0
55	369.0
56	329.0
57	306.5
58	284.0
59	284.0
60	744.5
61	1205.0
62	655.5
63	106.0
64	106.0
65	74.5
66	43.0
67	33.5
68	24.0
69	24.0
70	18.0
71	12.0
72	11.0
73	10.0
74	6.0
75	2.0
76	2.0
77	1.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.66193613786112	38.800000000000004
2	13.482007095793207	13.3
3	3.598580841358338	5.325
4	1.5205271160669032	3.0
5	0.8616320324379118	2.125
6	0.3547896604156108	1.05
7	0.15205271160669032	0.525
8	0.15205271160669032	0.6
9	0.05068423720223011	0.22499999999999998
>10	1.0136847440446022	9.2
>50	0.10136847440446022	3.8
>100	0.0	0.0
>500	0.05068423720223011	22.05
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCT	882	22.05	No Hit
AAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGAG	90	2.25	No Hit
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTAC	62	1.55	No Hit
GGTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTA	49	1.225	No Hit
AAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTC	30	0.75	No Hit
AAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTT	28	0.7000000000000001	No Hit
AAAAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGA	26	0.65	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGTGTCTCCCCCC	25	0.625	No Hit
GCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACA	20	0.5	No Hit
TGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	19	0.475	No Hit
GGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAAC	19	0.475	No Hit
AAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTT	18	0.44999999999999996	No Hit
GGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACAT	17	0.42500000000000004	No Hit
AAAAAACGCGCCCGAAGGCGCGTTGGCGATACACTCAATAT	15	0.375	No Hit
AAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCG	15	0.375	No Hit
AAAATAAGCCCGTGTAAGGGAGATTACACAGGCTAAGGAGG	12	0.3	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACGAGAGGACCGGGA	12	0.3	No Hit
AAAAAAAACCCGCTGATTAAGCGGGTTTTGAATTCTTGCTG	12	0.3	No Hit
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCC	11	0.27499999999999997	No Hit
AAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGT	10	0.25	No Hit
GGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAACC	10	0.25	No Hit
GGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAA	10	0.25	No Hit
TTGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGA	10	0.25	No Hit
GTGTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACC	9	0.22499999999999998	No Hit
GGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTC	8	0.2	No Hit
ATAAAAAGCCGCTCGGGAGTAAGCGAGCGGTGGTCAAATAA	8	0.2	No Hit
AAAATGCGCCACCGTGTTAGGGTGGCGCGTGCCCTGCTTTT	8	0.2	No Hit
AGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAA	7	0.17500000000000002	No Hit
ATGCCTGGCAGTTCCCCCCCAGATCGGAAGAGCACACGTCT	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGGCAC	7	0.17500000000000002	No Hit
GAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGAACCC	6	0.15	No Hit
AAAAAAATGGCGCACAATGTGCGCCATTTTTCACTTCACAG	6	0.15	No Hit
TGTACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCT	6	0.15	No Hit
GGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGC	6	0.15	No Hit
TTCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	6	0.15	No Hit
TTCACGAGGCGTGATGACGAGGCACTACGGTGCTGAAGCAA	6	0.15	No Hit
TTACATTCGCTTGCCAGCTGCGGACGGACACGCCACTAACA	6	0.15	No Hit
GGCGAAACCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGAC	5	0.125	No Hit
TCCACGCGATCTCTTTTGGGTTTGACCTCTCTTGATCCCCG	5	0.125	No Hit
CGTCGCCCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAA	5	0.125	No Hit
TTTTGCAACCCACTTTGTGATTCATGACTGGGGTGAAGTCG	5	0.125	No Hit
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAA	5	0.125	TruSeq Adapter, Index 6 (97% over 37bp)
TTTTGGGTTTGACCTCTCTTGATCCCCGTCCTAAGAGCGGA	5	0.125	No Hit
TCCTCGGTACTACATGCTTAGTCAGTCTTTACATTCGCTTG	5	0.125	No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATG	5	0.125	TruSeq Adapter, Index 6 (97% over 37bp)
TTTTGCGGCTTTTTACGAGGCCAACCGCCCCTCGGCATGCA	5	0.125	No Hit
TGGCGGAACGGACGGGACTCGAACCCGCGACCCCCTGCGTG	5	0.125	No Hit
GGATTTGCCTGGAAAATCAAGGCTGAGGCGTGATGACGAGG	5	0.125	No Hit
AGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGT	5	0.125	No Hit
AGGTTAAGCCTCACGGTTCATTAGTACGAGAGGACCGGGAT	5	0.125	No Hit
AGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCCCC	5	0.125	No Hit
AAGGGAATCTCCGAGATGCCGCCGCAGGCTGTAACCCTTGA	5	0.125	No Hit
TGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACA	5	0.125	No Hit
ATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.175	0.0	0.0	0.0	0.0
2	0.3	0.0	0.0	0.0	0.0
3	0.3	0.0	0.0	0.0	0.0
4	0.425	0.0	0.0	0.0	0.0
5	0.5	0.0	0.0	0.0	0.0
6	0.5	0.0	0.0	0.0	0.0
7	0.5	0.0	0.0	0.0	0.0
8	0.5	0.0	0.0	0.0	0.0
9	0.575	0.0	0.0	0.0	0.0
10	0.6	0.0	0.0	0.0	0.0
11	0.625	0.0	0.0	0.0	0.0
12	0.625	0.0	0.0	0.0	0.0
13	0.675	0.0	0.0	0.0	0.0
14	0.725	0.0	0.0	0.0	0.0
15	0.725	0.0	0.0	0.0	0.0
16	0.75	0.0	0.0	0.0	0.0
17	0.8	0.0	0.0	0.0	0.0
18	0.85	0.0	0.0	0.0	0.0
19	1.025	0.0	0.0	0.0	0.0
20	1.175	0.0	0.0	0.0	0.0
21	1.425	0.0	0.0	0.0	0.0
22	1.5	0.0	0.0	0.0	0.0
23	1.625	0.0	0.0	0.0	0.0
24	1.725	0.0	0.0	0.0	0.0
25	1.825	0.0	0.0	0.0	0.0
26	2.0	0.0	0.0	0.0	0.0
27	2.075	0.0	0.0	0.0	0.0
28	2.275	0.0	0.0	0.0	0.0
29	2.35	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTGGAG	90	0.0	33.055557	2
TGGTGGA	90	0.0	33.055557	1
AAATTCC	85	0.0	32.941174	34
AATTCCT	85	0.0	32.941174	35
GCGGGAG	95	0.0	31.315792	12
GTTGAAC	95	0.0	31.315792	18
GTCCGAA	95	0.0	31.315792	29
GCTGGCG	95	0.0	31.315792	8
GAGCTGG	95	0.0	31.315792	6
ACCCGCG	95	0.0	31.315792	23
TGGAGCT	95	0.0	31.315792	4
GGGAGTT	95	0.0	31.315792	14
GAGTTGA	95	0.0	31.315792	16
CTGGCGG	95	0.0	31.315792	9
GAACCCG	95	0.0	31.315792	21
GCGTCCG	95	0.0	31.315792	27
AGTTGAA	95	0.0	31.315792	17
TGGCGGG	95	0.0	31.315792	10
TCCGAAA	95	0.0	31.315792	30
GTGGAGC	95	0.0	31.315792	3
>>END_MODULE
SRR23332710 read2 length is 37-50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR23332710_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	37-50
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.854	11.0	11.0	37.0	11.0	37.0
2	23.139	25.0	11.0	37.0	11.0	37.0
3	25.7585	37.0	11.0	37.0	11.0	37.0
4	35.907	37.0	37.0	37.0	37.0	37.0
5	36.0665	37.0	37.0	37.0	37.0	37.0
6	35.806	37.0	37.0	37.0	37.0	37.0
7	35.73	37.0	37.0	37.0	37.0	37.0
8	35.8615	37.0	37.0	37.0	37.0	37.0
9	35.74	37.0	37.0	37.0	37.0	37.0
10	35.948	37.0	37.0	37.0	37.0	37.0
11	35.8145	37.0	37.0	37.0	37.0	37.0
12	35.915	37.0	37.0	37.0	37.0	37.0
13	35.89	37.0	37.0	37.0	37.0	37.0
14	35.9705	37.0	37.0	37.0	37.0	37.0
15	35.808	37.0	37.0	37.0	37.0	37.0
16	36.049	37.0	37.0	37.0	37.0	37.0
17	36.0855	37.0	37.0	37.0	37.0	37.0
18	36.1315	37.0	37.0	37.0	37.0	37.0
19	35.996	37.0	37.0	37.0	37.0	37.0
20	35.9945	37.0	37.0	37.0	37.0	37.0
21	35.9875	37.0	37.0	37.0	37.0	37.0
22	36.0805	37.0	37.0	37.0	37.0	37.0
23	36.0795	37.0	37.0	37.0	37.0	37.0
24	36.104	37.0	37.0	37.0	37.0	37.0
25	35.965	37.0	37.0	37.0	37.0	37.0
26	35.97775	37.0	37.0	37.0	37.0	37.0
27	35.93225	37.0	37.0	37.0	37.0	37.0
28	35.88	37.0	37.0	37.0	37.0	37.0
29	35.97025	37.0	37.0	37.0	37.0	37.0
30	35.9545	37.0	37.0	37.0	37.0	37.0
31	35.99025	37.0	37.0	37.0	37.0	37.0
32	35.8645	37.0	37.0	37.0	37.0	37.0
33	35.89375	37.0	37.0	37.0	37.0	37.0
34	36.083	37.0	37.0	37.0	37.0	37.0
35	36.12325	37.0	37.0	37.0	37.0	37.0
36	36.19775	37.0	37.0	37.0	37.0	37.0
37	36.045	37.0	37.0	37.0	37.0	37.0
38	36.062765691422854	37.0	37.0	37.0	37.0	37.0
39	35.94873718429607	37.0	37.0	37.0	37.0	37.0
40	35.945236309077266	37.0	37.0	37.0	37.0	37.0
41	35.722541906429825	37.0	37.0	37.0	37.0	37.0
42	35.91991991991992	37.0	37.0	37.0	37.0	37.0
43	35.72882205513785	37.0	37.0	37.0	37.0	37.0
44	35.94779730073848	37.0	37.0	37.0	37.0	37.0
45	35.744680851063826	37.0	37.0	37.0	37.0	37.0
46	35.78532901833873	37.0	37.0	37.0	37.0	37.0
47	35.95713888115781	37.0	37.0	37.0	37.0	37.0
48	35.942460317460316	37.0	37.0	37.0	37.0	37.0
49	35.9107498341075	37.0	37.0	37.0	37.0	37.0
50	35.550080775444265	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	3.0
21	3.0
22	10.0
23	12.0
24	10.0
25	14.0
26	15.0
27	23.0
28	31.0
29	47.0
30	46.0
31	54.0
32	99.0
33	162.0
34	412.0
35	1593.0
36	1200.0
37	264.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	38.1	30.049999999999997	31.85
2	38.0	34.075	12.5	15.425
3	33.125	37.8	14.174999999999999	14.899999999999999
4	78.825	8.5	6.125	6.550000000000001
5	64.525	16.425	11.25	7.8
6	57.550000000000004	19.025	9.2	14.224999999999998
7	44.574999999999996	31.95	9.575	13.900000000000002
8	35.625	22.2	13.475000000000001	28.7
9	20.925	34.075	30.075000000000003	14.924999999999999
10	43.35	26.1	15.45	15.1
11	25.900000000000002	42.325	17.075000000000003	14.7
12	22.85	25.624999999999996	32.65	18.875
13	30.675	13.4	36.725	19.2
14	28.449999999999996	17.575	24.4	29.575000000000003
15	28.075	16.950000000000003	42.9	12.075
16	46.275	22.35	20.424999999999997	10.95
17	32.925	28.000000000000004	18.625	20.45
18	15.725	41.075	25.025	18.175
19	18.325	19.900000000000002	36.85	24.925
20	15.024999999999999	23.35	35.075	26.55
21	18.15	35.875	13.65	32.324999999999996
22	38.95	27.675	14.025000000000002	19.35
23	21.25	37.0	17.875	23.875
24	23.474999999999998	21.95	12.875	41.699999999999996
25	45.65	16.900000000000002	12.85	24.6
26	41.01025256314079	21.13028257064266	12.253063265816454	25.6064016004001
27	43.51087771942986	22.355588897224308	19.42985746436609	14.703675918979744
28	31.065532766383193	28.88944472236118	26.113056528264135	13.931965982991496
29	19.204801200300075	16.129032258064516	48.31207801950488	16.35408852213053
30	20.4352176088044	16.283141570785393	52.30115057528765	10.98049024512256
31	19.42985746436609	21.255313828457115	38.359589897474365	20.955238809702426
32	38.14407203601801	14.532266133066532	19.109554777388695	28.214107053526767
33	22.88072018004501	15.478869717429358	13.178294573643413	48.46211552888222
34	31.89094547273637	21.635817908954476	19.559779889944974	26.91345672836418
35	15.153788447111777	42.91072768192048	19.129782445611404	22.80570142535634
36	14.528632158039509	43.23580895223806	22.95573893473368	19.279819954988746
37	18.3	46.725	10.45	24.525
38	22.930732683170792	17.30432608152038	10.40260065016254	49.362340585146285
39	28.60715178794699	18.704676169042262	11.927981995498875	40.76019004751188
40	19.229807451862964	25.506376594148538	16.779194798699677	38.48462115528882
41	18.989241931448586	42.256692519389546	23.042281711283465	15.711783837878409
42	26.076076076076077	34.434434434434436	22.27227227227227	17.217217217217218
43	38.771929824561404	22.606516290726816	15.764411027568922	22.857142857142858
44	42.526101349630764	14.565826330532214	19.65877259994907	23.249299719887954
45	22.074468085106382	15.50531914893617	35.0	27.420212765957448
46	35.19417475728155	19.82200647249191	24.730312837108954	20.253505933117584
47	26.280623608017816	16.119153674832962	20.79621380846325	36.80400890868597
48	24.610150269350722	32.66231925148852	22.313580946980434	20.41394953218032
49	24.991702621971456	10.985728509790906	45.76833720544308	18.254231662794556
50	38.933764135702745	13.045234248788368	26.453957996768985	21.567043618739902
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	1.0
20	1.0
21	0.5
22	0.0
23	0.0
24	0.5
25	2.0
26	3.0
27	2.5
28	2.5
29	11.0
30	21.5
31	24.5
32	25.5
33	30.5
34	37.5
35	42.5
36	45.0
37	42.5
38	42.5
39	62.0
40	91.5
41	114.5
42	117.0
43	110.0
44	114.5
45	127.5
46	138.0
47	153.5
48	164.0
49	174.0
50	215.5
51	412.0
52	592.5
53	729.5
54	888.5
55	886.0
56	798.5
57	618.0
58	436.5
59	267.5
60	147.5
61	119.5
62	90.5
63	70.5
64	52.5
65	42.5
66	28.5
67	17.5
68	10.5
69	4.5
70	1.5
71	4.0
72	5.5
73	3.0
74	0.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	1.5
82	3.0
83	3.0
84	3.0
85	1.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.025
27	0.025
28	0.05
29	0.025
30	0.05
31	0.025
32	0.05
33	0.025
34	0.05
35	0.025
36	0.025
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.027831895352073477
48	0.02834467120181406
49	0.033178500331785
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
37	1.0
38	0.0
39	0.0
40	2.0
41	1.0
42	6.0
43	63.0
44	167.0
45	52.0
46	115.0
47	65.0
48	514.0
49	538.0
50	2476.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.15
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.8649138712602	45.7
2	7.388939256572982	8.15
3	2.946509519492294	4.875
4	1.4959202175883952	3.3000000000000003
5	0.8612873980054397	2.375
6	0.9066183136899365	3.0
7	0.543970988213962	2.1
8	0.271985494106981	1.2
9	0.3173164097914778	1.575
>10	2.357207615593835	24.675
>50	0.0	0.0
>100	0.04533091568449683	3.05
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	122	3.05	No Hit
CTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	38	0.95	No Hit
CAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	37	0.9249999999999999	No Hit
TAAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	37	0.9249999999999999	No Hit
CGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	36	0.8999999999999999	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	34	0.8500000000000001	No Hit
AAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	32	0.8	No Hit
AGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	30	0.75	No Hit
AAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	30	0.75	No Hit
CGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	29	0.7250000000000001	No Hit
CAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	27	0.675	No Hit
AGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	26	0.65	No Hit
CGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	25	0.625	No Hit
AAAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	24	0.6	No Hit
CCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	22	0.5499999999999999	No Hit
AGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	22	0.5499999999999999	No Hit
TGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	22	0.5499999999999999	No Hit
AACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	21	0.525	No Hit
TACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	20	0.5	No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	20	0.5	No Hit
AGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	19	0.475	No Hit
TGAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	19	0.475	No Hit
CTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCT	18	0.44999999999999996	No Hit
AATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	18	0.44999999999999996	No Hit
CCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	18	0.44999999999999996	No Hit
TGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	17	0.42500000000000004	No Hit
CGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
CCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
CGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCT	16	0.4	No Hit
AAAGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	16	0.4	No Hit
CTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	16	0.4	No Hit
TGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	15	0.375	No Hit
AAAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAT	14	0.35000000000000003	No Hit
CACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
ATAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	14	0.35000000000000003	No Hit
AAAGGGAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	13	0.325	No Hit
TCAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
CGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
ACAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	13	0.325	No Hit
CGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	13	0.325	No Hit
AGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTA	12	0.3	No Hit
CATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
TGGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
CCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	12	0.3	No Hit
AGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	12	0.3	No Hit
AATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
TCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	11	0.27499999999999997	No Hit
TAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	11	0.27499999999999997	No Hit
AATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	11	0.27499999999999997	No Hit
TAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
CTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	10	0.25	No Hit
AGGGTTAGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGC	10	0.25	No Hit
ACCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
ACGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
CGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	9	0.22499999999999998	No Hit
AGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TTTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	9	0.22499999999999998	No Hit
AAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	9	0.22499999999999998	No Hit
TTCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
TAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	8	0.2	No Hit
CAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	8	0.2	No Hit
TGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	8	0.2	No Hit
CAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	8	0.2	No Hit
AGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCT	7	0.17500000000000002	No Hit
CGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TTGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
AAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	7	0.17500000000000002	No Hit
CGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TATGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
ACTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
TGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	7	0.17500000000000002	No Hit
CGAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	7	0.17500000000000002	No Hit
TCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	7	0.17500000000000002	No Hit
AAAGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTC	7	0.17500000000000002	No Hit
TGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCT	7	0.17500000000000002	No Hit
AAAGGGAGAGTCCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCA	6	0.15	No Hit
TGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TGTGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TTAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
CGCGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TATGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
AGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTG	6	0.15	No Hit
AGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AAAGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCT	6	0.15	No Hit
CAAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
CGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TGTGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	6	0.15	No Hit
TGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
AGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TAAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	6	0.15	No Hit
TGCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	6	0.15	No Hit
TCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	6	0.15	No Hit
CAGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
TCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	6	0.15	No Hit
AGTCGACAGGGAGTCAGACAACGAAGTGAACATCAGGATGATGACACT	5	0.125	No Hit
CCAGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
AGGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACGTTCC	5	0.125	Illumina Single End PCR Primer 1 (97% over 34bp)
ACGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGA	5	0.125	No Hit
TGGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
AACGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
ATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAG	5	0.125	No Hit
TAGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TGGGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TGAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CGCGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TATGGGAGACACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGC	5	0.125	No Hit
TTTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATG	5	0.125	No Hit
AGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
CGTGGGAGAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTT	5	0.125	No Hit
TTGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
AGACACGGTACTAATGAACCGTGAGGCTTAACCTTACGGAGGGC	5	0.125	No Hit
AGAGGGAGATTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.2	0.0	0.0	0.0	0.0
7	0.2	0.0	0.0	0.0	0.0
8	0.225	0.0	0.0	0.0	0.0
9	0.275	0.0	0.0	0.0	0.0
10	0.325	0.0	0.0	0.0	0.0
11	0.425	0.0	0.0	0.0	0.0
12	0.425	0.0	0.0	0.0	0.0
13	0.5	0.0	0.0	0.0	0.0
14	0.55	0.0	0.0	0.0	0.0
15	0.55	0.0	0.0	0.0	0.0
16	0.575	0.0	0.0	0.0	0.0
17	0.6	0.0	0.0	0.0	0.0
18	0.625	0.0	0.0	0.0	0.0
19	0.625	0.0	0.0	0.0	0.0
20	0.7	0.0	0.0	0.0	0.0
21	0.8	0.0	0.0	0.0	0.0
22	0.95	0.0	0.0	0.0	0.0
23	1.175	0.0	0.0	0.0	0.0
24	1.175	0.0	0.0	0.0	0.0
25	1.175	0.0	0.0	0.0	0.0
26	1.225	0.0	0.0	0.0	0.0
27	1.25	0.0	0.0	0.0	0.0
28	1.4	0.0	0.0	0.0	0.0
29	1.475	0.0	0.0	0.0	0.0
30	1.65	0.0	0.0	0.0	0.0
31	1.75	0.0	0.0	0.0	0.0
32	1.85	0.0	0.0	0.0	0.0
33	1.975	0.0	0.0	0.0	0.0
34	2.025	0.0	0.0	0.0	0.0
35	2.15	0.0	0.0	0.0	0.0
36	2.175	0.0	0.0	0.0	0.0
37	2.275	0.0	0.0	0.0	0.0
38	2.325	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCATG	80	0.0	58.229168	44
TGACACT	20	0.005594421	53.75	44
GGTGCAT	85	0.0	46.441658	43
CGGGGCT	20	7.786057E-4	43.0	3
AAGGGAG	20	7.786057E-4	43.0	2
AGGTGCA	85	0.0	40.470585	42
CCTTGTT	45	5.8682897E-5	39.814816	44
GCTGCGC	45	5.8682897E-5	39.814816	44
AAGGTGC	85	0.0	39.3464	41
CAAGGTG	85	0.0	38.28299	40
CCAAGGT	85	0.0	37.772545	39
AGGGAGA	40	3.5013727E-7	37.625	3
CCCAAGG	85	0.0	35.411762	38
ACCCAAG	85	0.0	35.411762	37
TGGGGCT	25	0.0023333877	34.4	3
GAAACCC	90	0.0	33.444447	34
GACGGGA	90	0.0	33.444447	22
GGATTTG	90	0.0	33.444447	26
GGCTGAT	90	0.0	33.444447	6
AACCCAA	90	0.0	33.444447	36
>>END_MODULE
Read 615267 spots for SRR23332710.sra
Written 615267 spots for SRR23332710.sra
Read 615267 spots for SRR23332710.sra
Written 615267 spots for SRR23332710.sra
Read 615267 spots for SRR23332710.sra
Written 615267 spots for SRR23332710.sra
Read 615273 spots for SRR23332710.sra
Written 615273 spots for SRR23332710.sra
Read 615267 spots for SRR23332710.sra
Written 615267 spots for SRR23332710.sra
Read 615267 spots for SRR23332710.sra
Written 615267 spots for SRR23332710.sra
Read 615267 spots for SRR23332710.sra
Written 615267 spots for SRR23332710.sra
Read 615267 spots for SRR23332710.sra
Written 615267 spots for SRR23332710.sra
SRR ids: ['SRR23332710.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_3v6p5hm3
SRR23332710.sra spots: 4922142
blocks: [[1, 615267], [615268, 1230534], [1230535, 1845801], [1845802, 2461068], [2461069, 3076335], [3076336, 3691602], [3691603, 4306869], [4306870, 4922142]]
SRR23332710 file size 598680
SRR23332710 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR23332710 SRR23332710_1.fastq SRR23332710_2.fastq
Input file:	SRR23332710_1.fastq
Paired file:	SRR23332710_2.fastq
trimmed:	SRR23332710-trimmed-pair1.fastq, SRR23332710-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 03:51:01 2026 >> started

Wed Mar 25 03:51:04 2026 >> done (2.922s)
4922142 read pairs processed; of these:
   2268 ( 0.05%) short read pairs filtered out after trimming by size control
   6965 ( 0.14%) empty read pairs filtered out after trimming by size control
4912909 (99.81%) read pairs available; of these:
  41421 ( 0.84%) trimmed read pairs available after processing
4871488 (99.16%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    651	  0.01%
 19	   1348	  0.03%
 20	   2382	  0.05%
 21	    921	  0.02%
 22	    647	  0.01%
 23	    711	  0.01%
 24	    839	  0.02%
 25	   1094	  0.02%
 26	    905	  0.02%
 27	   1028	  0.02%
 28	    864	  0.02%
 29	   1196	  0.02%
 30	    887	  0.02%
 31	   1230	  0.03%
 32	   1209	  0.02%
 33	   1478	  0.03%
 34	   1941	  0.04%
 35	   2059	  0.04%
 36	   1776	  0.04%
 37	   1994	  0.04%
 38	   2526	  0.05%
 39	   3011	  0.06%
 40	   8115	  0.17%
 41	   9744	  0.20%
 42	 273266	  5.56%
 43	 160373	  3.26%
 44	 527955	 10.75%
 45	3902759	 79.44%
4912909 reads passed initial QC


criterion=sequence-density
sequence-density=1.39
sequence-density-rank=1
fanout-score=2.50
fanout-score-rank=15
prefix-density=1.22
prefix-fanout=2.5
sequence=ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=41
fanout-score=16.15
fanout-score-rank=1
prefix-density=0.07
prefix-fanout=1.2
sequence=GGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCTCCCCCCA


criterion=sequence-density
sequence-density=24.34
sequence-density-rank=1
fanout-score=21.98
fanout-score-rank=8
prefix-density=19.85
prefix-fanout=22.0
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAA


criterion=fanout-score
sequence-density=0.74
sequence-density-rank=15
fanout-score=122.69
fanout-score-rank=1
prefix-density=7.46
prefix-fanout=12.2
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTACGGAGGGC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 987: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 988: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG -y GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAA -o SRR23332710 SRR23332710_1.fastq SRR23332710_2.fastq
Input file:	SRR23332710_1.fastq
Paired file:	SRR23332710_2.fastq
trimmed:	SRR23332710-trimmed-pair1.fastq, SRR23332710-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG
-- paired 3' end adapter sequence (-y):	GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed Mar 25 03:51:13 2026 >> started

Wed Mar 25 03:51:16 2026 >> done (2.675s)
4211065 read pairs processed; of these:
  62659 ( 1.49%) short read pairs filtered out after trimming by size control
   4729 ( 0.11%) empty read pairs filtered out after trimming by size control
4143677 (98.40%) read pairs available; of these:
   1299 ( 0.03%) trimmed read pairs available after processing
4142378 (99.97%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    532	  0.01%
 19	   1168	  0.03%
 20	   2039	  0.05%
 21	    792	  0.02%
 22	    543	  0.01%
 23	    606	  0.01%
 24	    733	  0.02%
 25	    951	  0.02%
 26	    761	  0.02%
 27	    874	  0.02%
 28	    732	  0.02%
 29	   1018	  0.02%
 30	    781	  0.02%
 31	   1057	  0.03%
 32	   1029	  0.02%
 33	   1288	  0.03%
 34	   1686	  0.04%
 35	   1744	  0.04%
 36	   1499	  0.04%
 37	   1694	  0.04%
 38	   2145	  0.05%
 39	   2585	  0.06%
 40	   6959	  0.17%
 41	   8346	  0.20%
 42	 232308	  5.61%
 43	 136933	  3.30%
 44	 431428	 10.41%
 45	3301446	 79.67%


criterion=sequence-density
sequence-density=1.26
sequence-density-rank=1
fanout-score=2.51
fanout-score-rank=14
prefix-density=1.22
prefix-fanout=2.5
sequence=ACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTCTCCCCCCAG


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=32
fanout-score=6.05
fanout-score-rank=1
prefix-density=0.09
prefix-fanout=2.0
sequence=GAACCCTTGGTCTTCCGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTAC


criterion=sequence-density
sequence-density=23.37
sequence-density-rank=1
fanout-score=21.64
fanout-score-rank=9
prefix-density=19.50
prefix-fanout=21.6
sequence=GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAACGGA


criterion=fanout-score
sequence-density=0.75
sequence-density-rank=15
fanout-score=122.09
fanout-score-rank=1
prefix-density=7.52
prefix-fanout=12.2
sequence=GGAGACACGGTACTAATGAACCGTGAGGCTTAACCTTACGGAGGGC
SRR23332710 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 25 03:51:31
                             Started mapping on |	Mar 25 03:51:31
                                    Finished on |	Mar 25 03:53:31
       Mapping speed, Million of reads per hour |	145.37

                          Number of input reads |	4845521
                      Average input read length |	89
                                    UNIQUE READS:
                   Uniquely mapped reads number |	3072965
                        Uniquely mapped reads % |	63.42%
                          Average mapped length |	87.77
                       Number of splices: Total |	124910
            Number of splices: Annotated (sjdb) |	484
                       Number of splices: GT/AG |	106037
                       Number of splices: GC/AG |	2778
                       Number of splices: AT/AC |	476
               Number of splices: Non-canonical |	15619
                      Mismatch rate per base, % |	0.24%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.33
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.01
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	806168
             % of reads mapped to multiple loci |	16.64%
        Number of reads mapped to too many loci |	649385
             % of reads mapped to too many loci |	13.40%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.23%
                     % of reads unmapped: other |	0.32%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	966397	966397	966397
N_multimapping	806168	806168	806168
N_noFeature	91188	2840413	170179
N_ambiguous	236147	36753	65017
UnstrandedReadsAssigned:2745630 PositiveStrandReadsAssigned:195799 NegativeStrandReadsAssigned:2837769
Dataset is classified negative stranded
MeadianReadLen=41 20thPercentileLength=41 echo kmer=37
SRR23332710 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR23332710-trimmed-pair1.fastq
                             SRR23332710-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,845,521 reads, 646,804 reads pseudoaligned
[quant] estimated average fragment length: 203.254
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 131 rounds

  4323 SRR23332710.ke.tsv
  4497 SRR23332710.se.tsv
  8820 total
==> SRR23332710.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	13.6715	255	3975.24
AAC73113	2463	2260.75	88	8.296
AAC73114	933	730.746	55	16.0411
AAC73115	1287	1084.75	97	19.0582
AAC73116	297	121.457	14	24.5665
AAC73117	777	574.892	28	10.3803
AAC73118	1431	1228.75	23	3.98936
AAC73119	954	751.746	470	133.249
AAC73120	588	387.187	84	46.2377

==> SRR23332710.se.tsv <==
b0001	412
b0002	87
b0003	44
b0004	95
b0005	14
b0006	29
b0007	24
b0008	469
b0009	96
b0010	41
SRR23332710 completed mapping pipeline successfully
