Starting /dee2/code/volunteer_pipeline.sh SRR32999252
    current disk space = 237727452
    free memory = 117227932 
SRR32999252 SRAfilesize
870c70510c3403cc6a3b9221d07c0e2b  SRR32999252.sra
SRR32999252.sra file validated
SRR32999252 is paired end
SRR32999252 is conventional basespace
SRR32999252 read1 length is 72-76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32999252_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	72-76
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.964	32.0	32.0	32.0	21.0	32.0
2	30.384	32.0	32.0	32.0	21.0	32.0
3	30.682	32.0	32.0	32.0	32.0	32.0
4	30.79425	32.0	32.0	32.0	32.0	32.0
5	30.81825	32.0	32.0	32.0	32.0	32.0
6	33.48375	36.0	36.0	36.0	21.0	36.0
7	33.773	36.0	36.0	36.0	32.0	36.0
8	33.1615	36.0	36.0	36.0	21.0	36.0
9	33.70175	36.0	36.0	36.0	32.0	36.0
10-11	33.866749999999996	36.0	36.0	36.0	32.0	36.0
12-13	33.696749999999994	36.0	36.0	36.0	32.0	36.0
14-15	33.803	36.0	36.0	36.0	32.0	36.0
16-17	33.691125	36.0	36.0	36.0	26.5	36.0
18-19	33.690375	36.0	36.0	36.0	29.5	36.0
20-21	33.615125	36.0	36.0	36.0	26.5	36.0
22-23	33.6215	36.0	36.0	36.0	27.0	36.0
24-25	33.510625000000005	36.0	36.0	36.0	24.0	36.0
26-27	33.540125	36.0	36.0	36.0	24.0	36.0
28-29	33.272625	36.0	36.0	36.0	17.5	36.0
30-31	33.432874999999996	36.0	36.0	36.0	24.0	36.0
32-33	32.946	36.0	36.0	36.0	14.0	36.0
34-35	33.325	36.0	36.0	36.0	20.5	36.0
36-37	33.24225	36.0	36.0	36.0	17.5	36.0
38-39	33.347375	36.0	36.0	36.0	20.5	36.0
40-41	33.113375000000005	36.0	36.0	36.0	21.0	36.0
42-43	33.069375	36.0	36.0	36.0	17.5	36.0
44-45	33.07125	36.0	36.0	36.0	17.5	36.0
46-47	33.023125	36.0	36.0	36.0	17.5	36.0
48-49	32.7335	36.0	36.0	36.0	14.0	36.0
50-51	32.628	36.0	32.0	36.0	17.5	36.0
52-53	32.4625	36.0	32.0	36.0	14.0	36.0
54-55	32.5655	36.0	32.0	36.0	14.0	36.0
56-57	32.322375	36.0	32.0	36.0	14.0	36.0
58-59	32.370125	36.0	32.0	36.0	14.0	36.0
60-61	32.325874999999996	36.0	32.0	36.0	14.0	36.0
62-63	32.168375	36.0	32.0	36.0	14.0	36.0
64-65	32.060375	36.0	32.0	36.0	14.0	36.0
66-67	31.84975	36.0	32.0	36.0	14.0	36.0
68-69	31.991625	36.0	32.0	36.0	14.0	36.0
70-71	31.694000000000003	36.0	32.0	36.0	14.0	36.0
72-73	31.610256817613212	36.0	32.0	36.0	14.0	36.0
74-75	31.59794062068387	36.0	32.0	36.0	14.0	36.0
76	28.991090520313612	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10-11	0.0
11101	12-13	0.0
11101	14-15	0.0
11101	16-17	0.0
11101	18-19	0.0
11101	20-21	0.0
11101	22-23	0.0
11101	24-25	0.0
11101	26-27	0.0
11101	28-29	0.0
11101	30-31	0.0
11101	32-33	0.0
11101	34-35	0.0
11101	36-37	0.0
11101	38-39	0.0
11101	40-41	0.0
11101	42-43	0.0
11101	44-45	0.0
11101	46-47	0.0
11101	48-49	0.0
11101	50-51	0.0
11101	52-53	0.0
11101	54-55	0.0
11101	56-57	0.0
11101	58-59	0.0
11101	60-61	0.0
11101	62-63	0.0
11101	64-65	0.0
11101	66-67	0.0
11101	68-69	0.0
11101	70-71	0.0
11101	72-73	0.0
11101	74-75	0.0
11101	76	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	4.0
22	9.0
23	12.0
24	27.0
25	41.0
26	70.0
27	112.0
28	165.0
29	222.0
30	263.0
31	357.0
32	434.0
33	550.0
34	846.0
35	888.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.325	44.425	23.375	16.875
2	27.175	41.099999999999994	18.125	13.600000000000001
3	29.175	40.550000000000004	16.475	13.8
4	28.825	27.05	28.525	15.6
5	27.700000000000003	25.724999999999998	31.025000000000002	15.55
6	46.025	20.200000000000003	18.0	15.775
7	33.300000000000004	33.425	16.625	16.650000000000002
8	29.175	43.075	14.45	13.3
9	43.375	23.3	16.675	16.650000000000002
10-11	37.9	30.625000000000004	14.475	17.0
12-13	31.7	20.2875	30.525000000000002	17.4875
14-15	34.925	19.75	26.224999999999998	19.1
16-17	32.175	26.8	21.512500000000003	19.5125
18-19	27.5125	26.55	19.775000000000002	26.1625
20-21	35.0875	22.8875	24.087500000000002	17.9375
22-23	39.050000000000004	21.099999999999998	17.9875	21.8625
24-25	28.5875	23.1	25.3	23.0125
26-27	35.6875	29.7	17.1125	17.5
28-29	29.325000000000003	31.874999999999996	22.037499999999998	16.7625
30-31	33.375	21.675	28.4125	16.537499999999998
32-33	30.3875	36.225	16.325	17.0625
34-35	34.275	24.1375	17.275	24.3125
36-37	29.5	21.1625	21.0	28.3375
38-39	41.5	22.162499999999998	17.7	18.637500000000003
40-41	34.875	21.912499999999998	16.45	26.7625
42-43	37.05	23.925	14.374999999999998	24.65
44-45	33.3125	29.725	17.175	19.787499999999998
46-47	37.025000000000006	25.424999999999997	14.3375	23.2125
48-49	27.8875	23.8625	20.724999999999998	27.525
50-51	28.287499999999998	34.725	17.025000000000002	19.9625
52-53	27.5625	30.4375	15.8875	26.1125
54-55	28.125	30.562499999999996	16.0625	25.25
56-57	30.3	28.249999999999996	20.05	21.4
58-59	27.675	29.2	15.8	27.325
60-61	31.112499999999997	32.0125	15.2375	21.637500000000003
62-63	26.5625	24.962500000000002	23.2875	25.1875
64-65	29.625	28.375	18.9	23.1
66-67	24.462500000000002	32.425	17.5625	25.55
68-69	33.137499999999996	24.55	16.825000000000003	25.4875
70-71	25.837500000000002	29.037499999999998	22.375	22.75
72-73	25.647117669125922	30.186319869951234	18.669501062898586	25.497061398024258
74-75	29.659105982225757	29.407083167528853	19.11394084096034	21.81987000928505
76	44.4048467569494	39.20171062009979	16.39344262295082	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.5
27	0.5
28	0.0
29	0.5
30	0.5
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	1.0
37	2.5
38	1.5
39	1.0
40	4.0
41	5.5
42	5.0
43	14.5
44	26.5
45	54.0
46	88.5
47	181.0
48	386.5
49	443.5
50	392.5
51	408.0
52	463.5
53	497.5
54	452.5
55	420.5
56	363.0
57	268.5
58	207.5
59	162.5
60	109.0
61	64.5
62	44.0
63	41.5
64	32.0
65	16.5
66	4.5
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
72	3.0
73	123.0
74	209.0
75	859.0
76	2806.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.96209456819071	53.075
2	7.932786244626808	10.15
3	3.5169988276670576	6.75
4	2.3055881203595154	5.8999999999999995
5	1.3286440015631107	4.25
6	0.6252442360296991	2.4
7	0.3907776475185619	1.7500000000000002
8	0.2735443532629933	1.4000000000000001
9	0.07815552950371239	0.44999999999999996
>10	0.5080109417741305	4.575
>50	0.039077764751856196	2.1999999999999997
>100	0.039077764751856196	7.1
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTA	284	7.1	No Hit
TAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCT	88	2.1999999999999997	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGATCGG	30	0.75	No Hit
AAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTC	19	0.475	No Hit
AGAGATGAGAATGTGCCTTCGGGAACCGTGAGACAGGTGCTGCATGGCTG	18	0.44999999999999996	No Hit
ATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAG	15	0.375	No Hit
AAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGG	12	0.3	No Hit
TGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGG	12	0.3	No Hit
AAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGT	12	0.3	No Hit
ATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAA	12	0.3	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACG	11	0.27499999999999997	No Hit
AGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGT	11	0.27499999999999997	No Hit
AAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACC	11	0.27499999999999997	No Hit
AAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCTCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGA	10	0.25	No Hit
AAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTC	10	0.25	No Hit
AGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCAC	9	0.22499999999999998	No Hit
TGGACGAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATT	9	0.22499999999999998	No Hit
GCAACGAGCGCAACCCTTATCCTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAA	8	0.2	No Hit
TCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTG	8	0.2	No Hit
ACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCG	8	0.2	No Hit
AATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTC	8	0.2	No Hit
AGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCC	8	0.2	No Hit
GGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCC	8	0.2	No Hit
CTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATG	8	0.2	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCAGATCGGAAGAGCACACGTCTGAACTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCAGATCGGAAG	7	0.17500000000000002	No Hit
AAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTA	7	0.17500000000000002	No Hit
AGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGT	7	0.17500000000000002	No Hit
GTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGA	7	0.17500000000000002	No Hit
TGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAA	7	0.17500000000000002	No Hit
AAACGATGTCGACTTGGAGGTTGTGCCCTTGAGGCGTGGCTTCCGGAGCT	7	0.17500000000000002	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAGATCGGAAGAGCACACGTCTGAACT	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACAGATCGGAAGAGCACACGT	7	0.17500000000000002	No Hit
TGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTC	7	0.17500000000000002	No Hit
GGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGC	6	0.15	No Hit
GGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAG	6	0.15	No Hit
AGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGG	6	0.15	No Hit
ACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCAT	6	0.15	No Hit
AGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACT	6	0.15	No Hit
GTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAG	6	0.15	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCAGATCGGAAGAG	6	0.15	No Hit
AAGCGGACCTCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCG	6	0.15	No Hit
AAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTG	6	0.15	No Hit
ACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAA	6	0.15	No Hit
ATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGG	6	0.15	No Hit
TGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCC	6	0.15	No Hit
AAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGAT	6	0.15	No Hit
TAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGC	6	0.15	No Hit
AGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCC	6	0.15	No Hit
CGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAAC	5	0.125	No Hit
GTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGG	5	0.125	No Hit
CAGAGATGAGAATGTGCCTTCGGGAACCGTGAGACAGGTGCTGCATGGCT	5	0.125	No Hit
ATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCC	5	0.125	No Hit
GAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGG	5	0.125	No Hit
CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG	5	0.125	No Hit
TTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAA	5	0.125	No Hit
TGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGA	5	0.125	No Hit
ATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCTCATAAAGTGCGT	5	0.125	No Hit
GTAAACGATGTCGACTTGGAGGTTGTGCCCTTGAGGCGTGGCTTCCGGAG	5	0.125	No Hit
ATGCAAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTG	5	0.125	No Hit
ATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCTCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAA	5	0.125	No Hit
AATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCTCATA	5	0.125	No Hit
AAGTCGAACGGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAA	5	0.125	No Hit
ATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACG	5	0.125	No Hit
AGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGT	5	0.125	No Hit
AGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAA	5	0.125	No Hit
AGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGC	5	0.125	No Hit
TTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAA	5	0.125	No Hit
TAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG	5	0.125	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCAGA	5	0.125	No Hit
CAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACA	5	0.125	No Hit
TACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGG	5	0.125	No Hit
TTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGAT	5	0.125	No Hit
CTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGA	5	0.125	No Hit
AACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAA	5	0.125	No Hit
ACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGC	5	0.125	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGTAGATCGG	5	0.125	No Hit
TGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGG	5	0.125	No Hit
GGGAACCGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAG	5	0.125	No Hit
CGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTA	5	0.125	No Hit
AATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAG	5	0.125	No Hit
AAATAGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTA	5	0.125	No Hit
AACGCGAAGAACCTTACCTGGTCTTGACATCCACGGAAGTTTTCAGAGAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.025	0.0	0.0	0.0	0.0
11	0.025	0.0	0.0	0.0	0.0
12	0.1	0.0	0.0	0.0	0.0
13	0.175	0.0	0.0	0.0	0.0
14	0.3	0.0	0.0	0.0	0.0
15	0.35	0.0	0.0	0.0	0.0
16	0.475	0.0	0.0	0.0	0.0
17	0.675	0.0	0.0	0.0	0.0
18	0.825	0.0	0.0	0.0	0.0
19	1.05	0.0	0.0	0.0	0.0
20	1.35	0.0	0.0	0.0	0.0
21	1.5	0.0	0.0	0.0	0.0
22	1.725	0.0	0.0	0.0	0.0
23	1.875	0.0	0.0	0.0	0.0
24	2.2	0.0	0.0	0.0	0.0
25	2.825	0.0	0.0	0.0	0.0
26	3.425	0.0	0.0	0.0	0.0
27	3.775	0.0	0.0	0.0	0.0
28	4.175	0.0	0.0	0.0	0.0
29	4.65	0.0	0.0	0.0	0.0
30	5.225	0.0	0.0	0.0	0.0
31	5.6	0.0	0.0	0.0	0.0
32	6.975	0.0	0.0	0.0	0.0
33	7.525	0.0	0.0	0.0	0.0
34	8.275	0.0	0.0	0.0	0.0
35	8.9	0.0	0.0	0.0	0.0
36	9.45	0.0	0.0	0.0	0.0
37	10.2	0.0	0.0	0.0	0.0
38	11.175	0.0	0.0	0.0	0.0
39	11.75	0.0	0.0	0.0	0.0
40	12.5	0.0	0.0	0.0	0.0
41	13.575	0.0	0.0	0.0	0.0
42	14.175	0.0	0.0	0.0	0.0
43	14.975	0.0	0.0	0.0	0.0
44	16.675	0.0	0.0	0.0	0.0
45	17.7	0.0	0.0	0.0	0.0
46	18.5	0.0	0.0	0.0	0.0
47	19.55	0.0	0.0	0.0	0.0
48	20.75	0.0	0.0	0.0	0.0
49	21.8	0.0	0.0	0.0	0.0
50	22.775	0.0	0.0	0.0	0.0
51	23.8	0.0	0.0	0.0	0.0
52	24.875	0.0	0.0	0.0	0.0
53	26.175	0.0	0.0	0.0	0.0
54	27.1	0.0	0.0	0.0	0.0
55	28.475	0.0	0.0	0.0	0.0
56	29.325	0.0	0.0	0.0	0.0
57	29.925	0.0	0.0	0.0	0.0
58	30.475	0.0	0.0	0.0	0.0
59	31.3	0.0	0.0	0.0	0.0
60	32.075	0.0	0.0	0.0	0.0
61	32.9	0.0	0.0	0.0	0.0
62	33.6	0.0	0.0	0.0	0.0
63	34.725	0.0	0.0	0.0	0.0
64	35.4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAATTG	15	0.00212396	69.5125	1
AAATTGA	50	4.6282366E-8	48.658752	1
AATTGAA	50	4.6282366E-8	48.658752	2
TGAAGAG	55	9.807627E-8	44.23523	5
ATTGAAG	55	9.807627E-8	44.23523	3
TTGAAGA	55	9.807627E-8	44.23523	4
GTTTGAT	60	1.9447907E-7	40.548958	11
AAGAGTT	60	1.9447907E-7	40.548958	7
ATGGCTC	60	1.9447907E-7	40.548958	19
TGATCAT	60	1.9447907E-7	40.548958	14
GAAGAGT	60	1.9447907E-7	40.548958	6
GAGTTTG	60	1.9447907E-7	40.548958	9
AGAGTTT	60	1.9447907E-7	40.548958	8
GGCTCAG	60	1.9447907E-7	40.548958	21
TTGATCA	60	1.9447907E-7	40.548958	13
GATCATG	60	1.9447907E-7	40.548958	15
AGTTTGA	60	1.9447907E-7	40.548958	10
TGGCTCA	60	1.9447907E-7	40.548958	20
TTTGATC	60	1.9447907E-7	40.548958	12
CTCAGAT	55	5.4351494E-6	37.915913	23
>>END_MODULE
SRR32999252 read2 length is 69-76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32999252_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	69-76
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.25625	32.0	32.0	32.0	21.0	32.0
2	30.17225	32.0	32.0	32.0	21.0	32.0
3	30.377	32.0	32.0	32.0	27.0	32.0
4	30.325	32.0	32.0	32.0	21.0	32.0
5	30.598	32.0	32.0	32.0	32.0	32.0
6	33.33725	36.0	36.0	36.0	21.0	36.0
7	33.677	36.0	36.0	36.0	32.0	36.0
8	33.69225	36.0	36.0	36.0	32.0	36.0
9	33.55	36.0	36.0	36.0	21.0	36.0
10-11	33.634125	36.0	36.0	36.0	26.5	36.0
12-13	33.639624999999995	36.0	36.0	36.0	32.0	36.0
14-15	33.492125	36.0	36.0	36.0	27.0	36.0
16-17	33.562375	36.0	36.0	36.0	29.5	36.0
18-19	33.554249999999996	36.0	36.0	36.0	29.5	36.0
20-21	33.453625	36.0	36.0	36.0	27.0	36.0
22-23	33.271	36.0	36.0	36.0	17.5	36.0
24-25	33.39175	36.0	36.0	36.0	24.0	36.0
26-27	33.287625	36.0	36.0	36.0	21.0	36.0
28-29	33.383875	36.0	36.0	36.0	24.0	36.0
30-31	33.127375	36.0	36.0	36.0	17.5	36.0
32-33	33.159125	36.0	36.0	36.0	21.0	36.0
34-35	33.074124999999995	36.0	36.0	36.0	21.0	36.0
36-37	32.959375	36.0	36.0	36.0	14.0	36.0
38-39	32.852374999999995	36.0	36.0	36.0	17.5	36.0
40-41	32.6045	36.0	32.0	36.0	14.0	36.0
42-43	32.5145	36.0	34.0	36.0	14.0	36.0
44-45	32.2545	36.0	32.0	36.0	14.0	36.0
46-47	32.337875	36.0	32.0	36.0	14.0	36.0
48-49	32.224000000000004	36.0	32.0	36.0	14.0	36.0
50-51	31.977874999999997	36.0	32.0	36.0	14.0	36.0
52-53	31.79875	36.0	32.0	36.0	14.0	36.0
54-55	31.650875	36.0	32.0	36.0	14.0	36.0
56-57	31.43175	36.0	32.0	36.0	14.0	36.0
58-59	31.337125	36.0	32.0	36.0	14.0	36.0
60-61	31.101125	36.0	32.0	36.0	14.0	36.0
62-63	31.000125	36.0	32.0	36.0	14.0	36.0
64-65	30.711750000000002	36.0	29.5	36.0	14.0	36.0
66-67	30.468249999999998	36.0	27.0	36.0	14.0	36.0
68-69	30.357625	36.0	27.0	36.0	14.0	36.0
70-71	29.922980745186294	36.0	27.0	36.0	14.0	36.0
72-73	29.529016060290832	34.0	27.0	36.0	14.0	36.0
74-75	29.19616303044576	34.0	27.0	36.0	14.0	36.0
76	25.351712328767125	27.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10-11	0.0
11101	12-13	0.0
11101	14-15	0.0
11101	16-17	0.0
11101	18-19	0.0
11101	20-21	0.0
11101	22-23	0.0
11101	24-25	0.0
11101	26-27	0.0
11101	28-29	0.0
11101	30-31	0.0
11101	32-33	0.0
11101	34-35	0.0
11101	36-37	0.0
11101	38-39	0.0
11101	40-41	0.0
11101	42-43	0.0
11101	44-45	0.0
11101	46-47	0.0
11101	48-49	0.0
11101	50-51	0.0
11101	52-53	0.0
11101	54-55	0.0
11101	56-57	0.0
11101	58-59	0.0
11101	60-61	0.0
11101	62-63	0.0
11101	64-65	0.0
11101	66-67	0.0
11101	68-69	0.0
11101	70-71	0.0
11101	72-73	0.0
11101	74-75	0.0
11101	76	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	6.0
19	7.0
20	13.0
21	17.0
22	20.0
23	36.0
24	52.0
25	89.0
26	113.0
27	153.0
28	182.0
29	238.0
30	285.0
31	341.0
32	383.0
33	565.0
34	785.0
35	714.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.324999999999996	35.275	12.725	12.675
2	18.65	22.525000000000002	16.900000000000002	41.925000000000004
3	15.85	17.95	31.275	34.925
4	21.075	19.625	26.3	33.0
5	19.35	17.599999999999998	25.874999999999996	37.175000000000004
6	15.65	21.275	25.324999999999996	37.75
7	20.375	22.8	22.975	33.85
8	20.5	20.225	27.450000000000003	31.825
9	19.1	18.2	25.85	36.85
10-11	19.25	19.2375	26.987499999999997	34.525
12-13	19.925	20.05	25.074999999999996	34.949999999999996
14-15	20.2375	24.5625	22.4375	32.7625
16-17	21.349999999999998	20.625	26.5875	31.4375
18-19	23.2125	20.9125	24.462500000000002	31.412499999999998
20-21	19.7	21.325	25.7375	33.2375
22-23	19.7375	23.05	23.45	33.7625
24-25	21.025	19.5625	26.650000000000002	32.7625
26-27	20.7875	21.587500000000002	25.924999999999997	31.7
28-29	19.287499999999998	24.2375	23.45	33.025
30-31	20.974999999999998	24.9125	25.137500000000003	28.975
32-33	21.775	21.575	23.1625	33.4875
34-35	23.775	22.787499999999998	25.5625	27.875
36-37	22.237499999999997	22.7375	24.1625	30.862499999999997
38-39	24.85	22.775000000000002	22.85	29.525000000000002
40-41	23.7375	23.375	26.6625	26.224999999999998
42-43	21.725	20.2625	24.6875	33.324999999999996
44-45	22.6	23.7875	24.025	29.5875
46-47	25.4	22.412499999999998	24.1125	28.075
48-49	25.650000000000002	23.025000000000002	23.6875	27.6375
50-51	25.424999999999997	24.15	24.3125	26.1125
52-53	28.762500000000003	24.637500000000003	22.125	24.474999999999998
54-55	27.425	22.8	23.625	26.150000000000002
56-57	27.474999999999998	22.5875	23.525	26.4125
58-59	27.375	23.425	21.5375	27.6625
60-61	28.95	23.825	22.35	24.875
62-63	26.974999999999998	24.3	22.275	26.450000000000003
64-65	29.512500000000003	23.525	22.85	24.1125
66-67	30.125	23.3375	22.7625	23.775
68-69	29.7125	23.45	21.987499999999997	24.85
70-71	30.83270817704426	24.58114528632158	23.305826456614152	21.280320080020005
72-73	29.47289345185927	24.42719419055966	23.951421059221232	22.148491298359836
74-75	30.312054900945228	25.54706720186456	24.770167033536193	19.37071086365402
76	38.082191780821915	30.753424657534246	31.164383561643838	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.5
12	1.0
13	1.0
14	0.5
15	0.0
16	0.0
17	0.0
18	0.5
19	1.5
20	3.0
21	3.5
22	2.0
23	1.5
24	1.5
25	1.0
26	2.5
27	2.5
28	1.0
29	1.0
30	1.5
31	2.0
32	3.5
33	4.5
34	3.5
35	3.0
36	3.5
37	4.0
38	4.0
39	3.5
40	3.5
41	8.5
42	13.5
43	22.5
44	44.0
45	68.5
46	83.5
47	115.5
48	217.0
49	330.0
50	382.5
51	436.5
52	508.0
53	530.0
54	518.5
55	485.5
56	393.5
57	302.5
58	254.5
59	195.0
60	132.5
61	83.5
62	51.0
63	38.5
64	27.0
65	17.5
66	8.5
67	3.0
68	1.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.5
75	1.0
76	0.5
77	0.0
78	0.0
79	0.0
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	1.0
89	1.5
90	0.5
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	2.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
69	1.0
70	0.0
71	2.0
72	7.0
73	45.0
74	167.0
75	858.0
76	2920.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.92500000000001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.93972506168488	58.825
2	9.340853013746916	13.25
3	3.1723651744800843	6.75
4	1.7271765949947127	4.9
5	0.7754670426506873	2.75
6	0.8107155445893549	3.45
7	0.31723651744800846	1.575
8	0.28198801550934083	1.6
9	0.035248501938667604	0.22499999999999998
>10	0.5992245329573492	6.675000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTC	30	0.75	No Hit
GCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAGATCGG	29	0.7250000000000001	No Hit
GTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAAT	23	0.575	No Hit
GACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGT	22	0.5499999999999999	No Hit
AGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACA	21	0.525	No Hit
GTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCA	15	0.375	No Hit
AACGTCAATGAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAA	14	0.35000000000000003	No Hit
AACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCT	14	0.35000000000000003	No Hit
GCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTC	13	0.325	No Hit
AATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATT	13	0.325	No Hit
GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGA	11	0.27499999999999997	No Hit
GCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTT	11	0.27499999999999997	No Hit
CCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATC	11	0.27499999999999997	No Hit
GCAGACTCCAATCCGGACTACGACGCACTTTAGATCGGAAGAGCGTCGTG	10	0.25	No Hit
GACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCG	10	0.25	No Hit
AACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCT	10	0.25	No Hit
AGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTA	10	0.25	No Hit
ACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAA	9	0.22499999999999998	No Hit
GCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAGA	8	0.2	No Hit
GCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAGATCG	8	0.2	No Hit
GCAGCACCTGTCTCACGGTTCCCGAAGGCACATTCTCATCTCTGAAAACT	8	0.2	No Hit
GTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCC	8	0.2	No Hit
GTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTG	8	0.2	No Hit
ACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGT	8	0.2	No Hit
GCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTG	8	0.2	No Hit
GGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCC	8	0.2	No Hit
ATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCG	7	0.17500000000000002	No Hit
ACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGA	7	0.17500000000000002	No Hit
ACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTAC	7	0.17500000000000002	No Hit
ACGTCAATGAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTT	7	0.17500000000000002	No Hit
CCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCA	7	0.17500000000000002	No Hit
GAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGC	7	0.17500000000000002	No Hit
GCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAG	7	0.17500000000000002	No Hit
GACGACAGCCATGCAGCACCTGTCTCACGGTTCCCGAAGGCACATTCTCA	7	0.17500000000000002	No Hit
GTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCA	7	0.17500000000000002	No Hit
GCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAA	6	0.15	No Hit
ACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAG	6	0.15	No Hit
GCAGACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCG	6	0.15	No Hit
AAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGC	6	0.15	No Hit
GTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGC	6	0.15	No Hit
ACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTA	6	0.15	No Hit
ACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTG	6	0.15	No Hit
GGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAG	6	0.15	No Hit
ACATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTC	6	0.15	No Hit
GCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCA	6	0.15	No Hit
AACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTG	6	0.15	No Hit
GATCCACGATTACTAGCGATTCCGACTTCATAGATCGGAAGAGCGTCGTG	6	0.15	No Hit
AACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGT	6	0.15	No Hit
ATTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAAT	6	0.15	No Hit
GAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTC	6	0.15	No Hit
ACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTT	6	0.15	No Hit
ACTCCACCGCTTGTGCGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGA	6	0.15	No Hit
GATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAAAC	6	0.15	No Hit
AATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCA	6	0.15	No Hit
GTAACGTCAATGAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTT	6	0.15	No Hit
GCCAGTATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGA	6	0.15	No Hit
GACTTGACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTT	6	0.15	No Hit
ACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCT	6	0.15	No Hit
AGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCAAAGAAGCAAGCTT	5	0.125	No Hit
GAAGCCACGCCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGGCGT	5	0.125	No Hit
ACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAG	5	0.125	No Hit
GACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACC	5	0.125	No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACT	5	0.125	No Hit
GCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAGATCGGAAG	5	0.125	No Hit
GCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAG	5	0.125	No Hit
AATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTT	5	0.125	No Hit
GTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGCCTG	5	0.125	No Hit
GTCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCA	5	0.125	No Hit
ACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGCACTTTA	5	0.125	No Hit
AGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACA	5	0.125	No Hit
ACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGA	5	0.125	No Hit
ATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCAAAGAAGCAAGCTTCTTC	5	0.125	No Hit
GTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTT	5	0.125	No Hit
AATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGG	5	0.125	No Hit
ACTCACCCGTCCGCCACTCGTCAGCGAAACAGCAAGCTGTTTCCTGTTACCGTTCGACTTGCATGTGTTAGGC	5	0.125	No Hit
ACCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAGATCGG	5	0.125	No Hit
GACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCA	5	0.125	No Hit
GTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTA	5	0.125	No Hit
ACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAA	5	0.125	No Hit
ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCATTTGAGTTTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.025	0.0	0.0	0.0	0.0
11	0.025	0.0	0.0	0.0	0.0
12	0.125	0.0	0.0	0.0	0.0
13	0.2	0.0	0.0	0.0	0.0
14	0.35	0.0	0.0	0.0	0.0
15	0.4	0.0	0.0	0.0	0.0
16	0.55	0.0	0.0	0.0	0.0
17	0.725	0.0	0.0	0.0	0.0
18	0.85	0.0	0.0	0.0	0.0
19	1.075	0.0	0.0	0.0	0.0
20	1.425	0.0	0.0	0.0	0.0
21	1.575	0.0	0.0	0.0	0.0
22	1.75	0.0	0.0	0.0	0.0
23	1.875	0.0	0.0	0.0	0.0
24	2.2	0.0	0.0	0.0	0.0
25	2.825	0.0	0.0	0.0	0.0
26	3.35	0.0	0.0	0.0	0.0
27	3.675	0.0	0.0	0.0	0.0
28	4.15	0.0	0.0	0.0	0.0
29	4.675	0.0	0.0	0.0	0.0
30	5.25	0.0	0.0	0.0	0.0
31	5.65	0.0	0.0	0.0	0.0
32	7.025	0.0	0.0	0.0	0.0
33	7.525	0.0	0.0	0.0	0.0
34	8.325	0.0	0.0	0.0	0.0
35	9.025	0.0	0.0	0.0	0.0
36	9.625	0.0	0.0	0.0	0.0
37	10.325	0.0	0.0	0.0	0.0
38	11.3	0.0	0.0	0.0	0.0
39	11.875	0.0	0.0	0.0	0.0
40	12.65	0.0	0.0	0.0	0.0
41	13.75	0.0	0.0	0.0	0.0
42	14.35	0.0	0.0	0.0	0.0
43	15.175	0.0	0.0	0.0	0.0
44	16.8	0.0	0.0	0.0	0.0
45	17.8	0.0	0.0	0.0	0.0
46	18.6	0.0	0.0	0.0	0.0
47	19.6	0.0	0.0	0.0	0.0
48	20.825	0.0	0.0	0.0	0.0
49	21.825	0.0	0.0	0.0	0.0
50	22.85	0.0	0.0	0.0	0.0
51	23.875	0.0	0.0	0.0	0.0
52	24.925	0.0	0.0	0.0	0.0
53	26.25	0.0	0.0	0.0	0.0
54	27.325	0.0	0.0	0.0	0.0
55	28.7	0.0	0.0	0.0	0.0
56	29.525	0.0	0.0	0.0	0.0
57	30.125	0.0	0.0	0.0	0.0
58	30.725	0.0	0.0	0.0	0.0
59	31.6	0.0	0.0	0.0	0.0
60	32.4	0.0	0.0	0.0	0.0
61	33.125	0.0	0.0	0.0	0.0
62	33.875	0.0	0.0	0.0	0.0
63	34.9	0.0	0.0	0.0	0.0
64	35.55	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 781565 spots for SRR32999252.sra
Written 781565 spots for SRR32999252.sra
Read 781565 spots for SRR32999252.sra
Written 781565 spots for SRR32999252.sra
Read 781565 spots for SRR32999252.sra
Written 781565 spots for SRR32999252.sra
Read 781565 spots for SRR32999252.sra
Written 781565 spots for SRR32999252.sra
Read 781569 spots for SRR32999252.sra
Written 781569 spots for SRR32999252.sra
Read 781565 spots for SRR32999252.sra
Written 781565 spots for SRR32999252.sra
Read 781565 spots for SRR32999252.sra
Written 781565 spots for SRR32999252.sra
Read 781565 spots for SRR32999252.sra
Written 781565 spots for SRR32999252.sra
SRR ids: ['SRR32999252.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_c_3qzet5
SRR32999252.sra spots: 6252524
blocks: [[1, 781565], [781566, 1563130], [1563131, 2344695], [2344696, 3126260], [3126261, 3907825], [3907826, 4689390], [4689391, 5470955], [5470956, 6252524]]
SRR32999252 file size 1399488
SRR32999252 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32999252 SRR32999252_1.fastq SRR32999252_2.fastq
Input file:	SRR32999252_1.fastq
Paired file:	SRR32999252_2.fastq
trimmed:	SRR32999252-trimmed-pair1.fastq, SRR32999252-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu May 21 17:01:40 2026 >> started

Thu May 21 17:01:47 2026 >> done (7.203s)
6252524 read pairs processed; of these:
 208128 ( 3.33%) short read pairs filtered out after trimming by size control
    540 ( 0.01%) empty read pairs filtered out after trimming by size control
6043856 (96.66%) read pairs available; of these:
3733982 (61.78%) trimmed read pairs available after processing
2309874 (38.22%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  43638	  0.72%
 19	  42305	  0.70%
 20	  45922	  0.76%
 21	  50411	  0.83%
 22	  51039	  0.84%
 23	  46628	  0.77%
 24	  59081	  0.98%
 25	  57497	  0.95%
 26	  55623	  0.92%
 27	  69357	  1.15%
 28	  63091	  1.04%
 29	  66965	  1.11%
 30	  66384	  1.10%
 31	 100297	  1.66%
 32	  62398	  1.03%
 33	  74198	  1.23%
 34	  69815	  1.16%
 35	  67294	  1.11%
 36	  70046	  1.16%
 37	  82524	  1.37%
 38	  67683	  1.12%
 39	  66797	  1.11%
 40	  92794	  1.54%
 41	  67910	  1.12%
 42	  75083	  1.24%
 43	 120361	  1.99%
 44	  89701	  1.48%
 45	  73614	  1.22%
 46	  76207	  1.26%
 47	  79418	  1.31%
 48	  91599	  1.52%
 49	 109625	  1.81%
 50	  75795	  1.25%
 51	  79412	  1.31%
 52	 103622	  1.71%
 53	  66866	  1.11%
 54	  77833	  1.29%
 55	  64917	  1.07%
 56	  56570	  0.94%
 57	  54660	  0.90%
 58	  70305	  1.16%
 59	  61696	  1.02%
 60	  56464	  0.93%
 61	  51120	  0.85%
 62	  57014	  0.94%
 63	  59268	  0.98%
 64	  52882	  0.87%
 65	  44604	  0.74%
 66	  43052	  0.71%
 67	  42472	  0.70%
 68	  48893	  0.81%
 69	  46814	  0.77%
 70	  48309	  0.80%
 71	  40867	  0.68%
 72	  43639	  0.72%
 73	  58671	  0.97%
 74	 213168	  3.53%
 75	1147097	 18.98%
 76	1022541	 16.92%
6043856 reads passed initial QC


criterion=sequence-density
sequence-density=5.24
sequence-density-rank=1
fanout-score=2.05
fanout-score-rank=24
prefix-density=4.97
prefix-fanout=2.1
sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG


criterion=fanout-score
sequence-density=1.03
sequence-density-rank=2
fanout-score=46.96
fanout-score-rank=1
prefix-density=1.23
prefix-fanout=39.4
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGATCTATCTCGTATGCCGT


criterion=sequence-density
sequence-density=2.95
sequence-density-rank=1
fanout-score=2.29
fanout-score-rank=25
prefix-density=6.50
prefix-fanout=1.0
sequence=CACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=39
fanout-score=1019.39
fanout-score-rank=1
prefix-density=6.92
prefix-fanout=1.5
sequence=TCCCAGACATTGCTCACCCGTCC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG -y CACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTA -o SRR32999252 SRR32999252_1.fastq SRR32999252_2.fastq
Input file:	SRR32999252_1.fastq
Paired file:	SRR32999252_2.fastq
trimmed:	SRR32999252-trimmed-pair1.fastq, SRR32999252-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACT
-- paired 3' end adapter sequence (-y):	CACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu May 21 17:02:01 2026 >> started

Thu May 21 17:02:06 2026 >> done (5.513s)
3626314 read pairs processed; of these:
   2787 ( 0.08%) short read pairs filtered out after trimming by size control
  18879 ( 0.52%) empty read pairs filtered out after trimming by size control
3604648 (99.40%) read pairs available; of these:
    298 ( 0.01%) trimmed read pairs available after processing
3604350 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  26326	  0.73%
 19	  25421	  0.71%
 20	  27432	  0.76%
 21	  29975	  0.83%
 22	  30592	  0.85%
 23	  27918	  0.77%
 24	  35597	  0.99%
 25	  34182	  0.95%
 26	  33058	  0.92%
 27	  41464	  1.15%
 28	  37785	  1.05%
 29	  40224	  1.12%
 30	  39500	  1.10%
 31	  60031	  1.67%
 32	  37331	  1.04%
 33	  44284	  1.23%
 34	  41837	  1.16%
 35	  40308	  1.12%
 36	  42026	  1.17%
 37	  49112	  1.36%
 38	  40535	  1.12%
 39	  39967	  1.11%
 40	  55088	  1.53%
 41	  40428	  1.12%
 42	  44662	  1.24%
 43	  71735	  1.99%
 44	  53411	  1.48%
 45	  44002	  1.22%
 46	  45505	  1.26%
 47	  47396	  1.31%
 48	  54935	  1.52%
 49	  65730	  1.82%
 50	  45569	  1.26%
 51	  47327	  1.31%
 52	  61998	  1.72%
 53	  40074	  1.11%
 54	  46863	  1.30%
 55	  38544	  1.07%
 56	  33655	  0.93%
 57	  32466	  0.90%
 58	  41865	  1.16%
 59	  36494	  1.01%
 60	  33851	  0.94%
 61	  30095	  0.83%
 62	  34042	  0.94%
 63	  35359	  0.98%
 64	  31507	  0.87%
 65	  26578	  0.74%
 66	  25922	  0.72%
 67	  25363	  0.70%
 68	  29076	  0.81%
 69	  28089	  0.78%
 70	  28807	  0.80%
 71	  24245	  0.67%
 72	  26059	  0.72%
 73	  35064	  0.97%
 74	 127420	  3.53%
 75	 682240	 18.93%
 76	 608279	 16.87%


criterion=sequence-density
sequence-density=5.05
sequence-density-rank=1
fanout-score=2.05
fanout-score-rank=23
prefix-density=4.97
prefix-fanout=2.1
sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG


criterion=fanout-score
sequence-density=1.11
sequence-density-rank=2
fanout-score=47.71
fanout-score-rank=1
prefix-density=1.34
prefix-fanout=39.6
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGATCTATCTCGTATGCCGT


criterion=sequence-density
sequence-density=2.95
sequence-density-rank=1
fanout-score=2.25
fanout-score-rank=26
prefix-density=6.38
prefix-fanout=1.0
sequence=CACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=40
fanout-score=1038.59
fanout-score-rank=1
prefix-density=6.66
prefix-fanout=1.5
sequence=TCCCAGACATTGCTCACCCGTCC
SRR32999252 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 21 17:02:51
                             Started mapping on |	May 21 17:02:51
                                    Finished on |	May 21 17:06:53
       Mapping speed, Million of reads per hour |	89.59

                          Number of input reads |	6022190
                      Average input read length |	113
                                    UNIQUE READS:
                   Uniquely mapped reads number |	364408
                        Uniquely mapped reads % |	6.05%
                          Average mapped length |	122.48
                       Number of splices: Total |	36371
            Number of splices: Annotated (sjdb) |	7
                       Number of splices: GT/AG |	28934
                       Number of splices: GC/AG |	1144
                       Number of splices: AT/AC |	28
               Number of splices: Non-canonical |	6265
                      Mismatch rate per base, % |	0.84%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.15
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.10
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	5290878
             % of reads mapped to multiple loci |	87.86%
        Number of reads mapped to too many loci |	24743
             % of reads mapped to too many loci |	0.41%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.53%
                     % of reads unmapped: other |	2.15%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	366904	366904	366904
N_multimapping	5290878	5290878	5290878
N_noFeature	1057	8544	348308
N_ambiguous	17382	8599	2300
UnstrandedReadsAssigned:345969 PositiveStrandReadsAssigned:347265 NegativeStrandReadsAssigned:13800
Dataset is classified positive stranded
MeadianReadLen=60 20thPercentileLength=37 echo kmer=33
SRR32999252 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR32999252-trimmed-pair1.fastq
                             SRR32999252-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 6,022,190 reads, 887 reads pseudoaligned
[quant] estimated average fragment length: 64.2936
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 52 rounds

  4323 SRR32999252.ke.tsv
  4497 SRR32999252.se.tsv
  8820 total
==> SRR32999252.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	18.7578	0	0
AAC73113	2463	2399.71	0	0
AAC73114	933	869.706	0	0
AAC73115	1287	1223.71	0	0
AAC73116	297	234.135	0	0
AAC73117	777	713.706	0	0
AAC73118	1431	1367.71	0	0
AAC73119	954	890.706	1	256.815
AAC73120	588	524.706	0	0

==> SRR32999252.se.tsv <==
b0001	0
b0002	0
b0003	0
b0004	0
b0005	0
b0006	0
b0007	0
b0008	2
b0009	0
b0010	0
SRR32999252 completed mapping pipeline successfully
