Starting /dee2/code/volunteer_pipeline.sh SRR32999257
    current disk space = 237672832
    free memory = 116518656 
SRR32999257 SRAfilesize
c40ef92eaf36c38d3c3d544437816846  SRR32999257.sra
SRR32999257.sra file validated
SRR32999257 is paired end
SRR32999257 is conventional basespace
SRR32999257 read1 length is 72-76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32999257_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	72-76
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.29475	32.0	32.0	32.0	21.0	32.0
2	30.77675	32.0	32.0	32.0	32.0	32.0
3	30.735	32.0	32.0	32.0	32.0	32.0
4	30.8955	32.0	32.0	32.0	32.0	32.0
5	30.82125	32.0	32.0	32.0	32.0	32.0
6	33.42975	36.0	36.0	36.0	21.0	36.0
7	33.957	36.0	36.0	36.0	32.0	36.0
8	33.456	36.0	36.0	36.0	21.0	36.0
9	33.79425	36.0	36.0	36.0	32.0	36.0
10-11	34.028125	36.0	36.0	36.0	32.0	36.0
12-13	33.833625	36.0	36.0	36.0	32.0	36.0
14-15	34.12075	36.0	36.0	36.0	32.0	36.0
16-17	33.895375	36.0	36.0	36.0	32.0	36.0
18-19	33.831875	36.0	36.0	36.0	32.0	36.0
20-21	33.89525	36.0	36.0	36.0	32.0	36.0
22-23	33.92125	36.0	36.0	36.0	32.0	36.0
24-25	33.843125	36.0	36.0	36.0	32.0	36.0
26-27	33.643	36.0	36.0	36.0	27.0	36.0
28-29	33.5415	36.0	36.0	36.0	24.0	36.0
30-31	33.59125	36.0	36.0	36.0	27.0	36.0
32-33	33.259125	36.0	36.0	36.0	20.5	36.0
34-35	33.401250000000005	36.0	36.0	36.0	20.5	36.0
36-37	33.606375	36.0	36.0	36.0	27.0	36.0
38-39	33.491	36.0	36.0	36.0	24.0	36.0
40-41	33.288250000000005	36.0	36.0	36.0	21.0	36.0
42-43	33.2965	36.0	36.0	36.0	21.0	36.0
44-45	33.296375	36.0	36.0	36.0	21.0	36.0
46-47	33.120000000000005	36.0	36.0	36.0	17.5	36.0
48-49	33.01025	36.0	36.0	36.0	21.0	36.0
50-51	32.793	36.0	32.0	36.0	17.5	36.0
52-53	32.694	36.0	32.0	36.0	17.5	36.0
54-55	32.766875	36.0	32.0	36.0	21.0	36.0
56-57	32.6055	36.0	32.0	36.0	14.0	36.0
58-59	32.677125000000004	36.0	32.0	36.0	14.0	36.0
60-61	32.622875	36.0	32.0	36.0	14.0	36.0
62-63	32.539249999999996	36.0	32.0	36.0	14.0	36.0
64-65	32.328500000000005	36.0	32.0	36.0	14.0	36.0
66-67	32.3605	36.0	32.0	36.0	14.0	36.0
68-69	32.226	36.0	32.0	36.0	14.0	36.0
70-71	32.105625	36.0	32.0	36.0	14.0	36.0
72-73	32.135508970992746	36.0	32.0	36.0	14.0	36.0
74-75	31.98583425651632	36.0	32.0	36.0	14.0	36.0
76	30.017085076708508	36.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10-11	0.0
11101	12-13	0.0
11101	14-15	0.0
11101	16-17	0.0
11101	18-19	0.0
11101	20-21	0.0
11101	22-23	0.0
11101	24-25	0.0
11101	26-27	0.0
11101	28-29	0.0
11101	30-31	0.0
11101	32-33	0.0
11101	34-35	0.0
11101	36-37	0.0
11101	38-39	0.0
11101	40-41	0.0
11101	42-43	0.0
11101	44-45	0.0
11101	46-47	0.0
11101	48-49	0.0
11101	50-51	0.0
11101	52-53	0.0
11101	54-55	0.0
11101	56-57	0.0
11101	58-59	0.0
11101	60-61	0.0
11101	62-63	0.0
11101	64-65	0.0
11101	66-67	0.0
11101	68-69	0.0
11101	70-71	0.0
11101	72-73	0.0
11101	74-75	0.0
11101	76	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	3.0
22	5.0
23	14.0
24	20.0
25	43.0
26	70.0
27	106.0
28	144.0
29	191.0
30	259.0
31	294.0
32	380.0
33	503.0
34	835.0
35	1133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.45	38.35	27.6	19.6
2	27.675	39.15	19.775000000000002	13.4
3	30.2	39.975	15.65	14.174999999999999
4	26.650000000000002	28.799999999999997	28.625	15.925
5	27.375	25.374999999999996	32.475	14.774999999999999
6	45.775	19.5	19.650000000000002	15.075
7	33.825	35.4	15.299999999999999	15.475
8	28.349999999999998	42.25	14.875	14.524999999999999
9	43.95	23.724999999999998	16.3	16.025
10-11	37.875	30.312499999999996	14.762500000000001	17.05
12-13	32.25	18.6875	31.55	17.5125
14-15	36.0375	20.4	25.7375	17.825
16-17	32.1375	28.9125	22.6	16.35
18-19	27.200000000000003	27.6375	18.9375	26.224999999999998
20-21	34.625	23.325000000000003	24.587500000000002	17.4625
22-23	39.775	21.3125	16.35	22.5625
24-25	28.4125	21.175	26.174999999999997	24.2375
26-27	35.125	30.375000000000004	16.900000000000002	17.599999999999998
28-29	28.1125	33.0625	22.1375	16.6875
30-31	31.374999999999996	23.200000000000003	27.474999999999998	17.95
32-33	30.225	35.05	16.9625	17.7625
34-35	35.825	23.5375	16.2125	24.425
36-37	29.012500000000003	21.25	21.8	27.9375
38-39	41.125	21.9375	18.075	18.862499999999997
40-41	34.0375	22.8	15.137500000000001	28.025
42-43	37.4375	23.2375	14.475	24.85
44-45	33.2125	29.625	15.45	21.712500000000002
46-47	37.3125	24.25	15.174999999999999	23.2625
48-49	27.250000000000004	24.349999999999998	20.724999999999998	27.675
50-51	27.6875	35.0	17.2125	20.1
52-53	28.8875	30.587500000000002	14.75	25.775
54-55	29.362500000000004	31.0	15.174999999999999	24.462500000000002
56-57	30.1875	28.175	20.125	21.512500000000003
58-59	27.375	28.812500000000004	16.3	27.5125
60-61	29.575000000000003	32.625	15.65	22.15
62-63	27.5625	23.9	22.975	25.5625
64-65	31.35	27.474999999999998	18.712500000000002	22.4625
66-67	24.9	31.775	18.0625	25.2625
68-69	32.587500000000006	25.2875	17.175	24.95
70-71	27.1125	28.212500000000002	22.875	21.8
72-73	26.215776972121514	30.466308288536066	17.92724090511314	25.390673834229275
74-75	30.374934452018877	29.48348190875721	19.24488725747247	20.896696381751443
76	45.5020920502092	38.56345885634588	15.934449093444911	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.5
28	0.5
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.5
37	1.5
38	1.5
39	1.0
40	1.5
41	2.5
42	3.5
43	13.0
44	35.0
45	56.5
46	73.5
47	175.0
48	411.0
49	474.0
50	394.5
51	406.5
52	444.5
53	464.5
54	447.0
55	435.5
56	382.0
57	290.0
58	228.5
59	176.0
60	113.0
61	65.0
62	44.0
63	43.0
64	36.5
65	13.5
66	1.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	1.5
94	1.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
72	1.0
73	100.0
74	170.0
75	861.0
76	2868.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.175000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.81446669391092	50.05
2	8.58193706579485	10.5
3	3.7188393951777683	6.825
4	1.9207192480588478	4.7
5	1.0625255414793624	3.25
6	1.2259910093992643	4.5
7	0.4495300367797303	1.925
8	0.2451982018798529	1.2
9	0.28606456885982834	1.575
>10	0.6129955046996322	5.625
>50	0.04086636697997548	2.275
>100	0.04086636697997548	7.575
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTA	303	7.575	No Hit
TAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCT	91	2.275	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGATCGG	31	0.775	No Hit
AAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACC	22	0.5499999999999999	No Hit
TGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGG	18	0.44999999999999996	No Hit
ATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCA	17	0.42500000000000004	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCAGA	17	0.42500000000000004	No Hit
TGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTC	16	0.4	No Hit
ATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAG	14	0.35000000000000003	No Hit
TGGACGAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATT	13	0.325	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCAGATCGGAAGAGCACACGTCTGAACTC	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCAGATCGGAAG	12	0.3	No Hit
TTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGAT	12	0.3	No Hit
AGAGATGAGAATGTGCCTTCGGGAACCGTGAGACAGGTGCTGCATGGCTG	11	0.27499999999999997	No Hit
AAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTA	10	0.25	No Hit
ACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCG	10	0.25	No Hit
AGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGT	10	0.25	No Hit
CTGGACGAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGAT	9	0.22499999999999998	No Hit
AGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGG	9	0.22499999999999998	No Hit
TGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTAC	9	0.22499999999999998	No Hit
GTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGA	9	0.22499999999999998	No Hit
TGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGG	9	0.22499999999999998	No Hit
TAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCAGATCGGAAGAGCACACGTCTGAACTC	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAA	9	0.22499999999999998	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACG	8	0.2	No Hit
CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG	8	0.2	No Hit
AACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGG	8	0.2	No Hit
AATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTC	8	0.2	No Hit
TCGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC	8	0.2	No Hit
CGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGG	8	0.2	No Hit
GGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGC	7	0.17500000000000002	No Hit
ATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGA	7	0.17500000000000002	No Hit
CGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATA	7	0.17500000000000002	No Hit
TAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGG	7	0.17500000000000002	No Hit
ATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCG	7	0.17500000000000002	No Hit
CAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGG	7	0.17500000000000002	No Hit
TCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCC	7	0.17500000000000002	No Hit
ATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTG	7	0.17500000000000002	No Hit
CTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAA	7	0.17500000000000002	No Hit
TGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATA	7	0.17500000000000002	No Hit
TACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGG	7	0.17500000000000002	No Hit
TTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATG	6	0.15	No Hit
TACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGC	6	0.15	No Hit
GGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAG	6	0.15	No Hit
TGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGAC	6	0.15	No Hit
CTTATCCTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCA	6	0.15	No Hit
AGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGT	6	0.15	No Hit
ACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGG	6	0.15	No Hit
ACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCT	6	0.15	No Hit
CGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCT	6	0.15	No Hit
CGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA	6	0.15	No Hit
TACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCTC	6	0.15	No Hit
GACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGG	6	0.15	No Hit
GATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACG	6	0.15	No Hit
AGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAA	6	0.15	No Hit
ATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGG	6	0.15	No Hit
AGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGC	6	0.15	No Hit
AGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAG	6	0.15	No Hit
ACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	6	0.15	No Hit
GAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATA	6	0.15	No Hit
TGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCC	6	0.15	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAGATCGGAAGAGCACACGTCTGAACT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTAC	6	0.15	No Hit
CGGAAGTTTTCAGAGATGAGAATGTGCCTTCGGGAACCGTGAGACAGGTG	6	0.15	No Hit
AAGTCCCGCAACGAGCGCAACCCTTATCCTTTGTTGCCAGCGGTCCGGCC	6	0.15	No Hit
TAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACGAGCGCAACCCTTATCCTTTGTTGCCAGCGGTCCGGCCGGGAACTCA	6	0.15	No Hit
AAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGT	6	0.15	No Hit
CTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATG	6	0.15	No Hit
TGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTGTA	6	0.15	No Hit
CGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTA	6	0.15	No Hit
AACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCG	5	0.125	No Hit
CGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATA	5	0.125	No Hit
CTCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATG	5	0.125	No Hit
ATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGC	5	0.125	No Hit
TTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCC	5	0.125	No Hit
AGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGT	5	0.125	No Hit
AAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCTCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGA	5	0.125	No Hit
TGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTC	5	0.125	No Hit
GGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATAC	5	0.125	No Hit
CAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAG	5	0.125	No Hit
CTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGCCCTTGAG	5	0.125	No Hit
CTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGG	5	0.125	No Hit
AAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACT	5	0.125	No Hit
TTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACT	5	0.125	No Hit
CAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGG	5	0.125	No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA	5	0.125	No Hit
AAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAA	5	0.125	No Hit
CACGGAAGTTTTCAGAGATGAGAATGTGCCTTCGGGAACCGTGAGACAGG	5	0.125	No Hit
TACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGG	5	0.125	No Hit
TGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGG	5	0.125	No Hit
GGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCC	5	0.125	No Hit
AGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACG	5	0.125	No Hit
TGGTCTTGACATCCACGGAAGTTTTCAGAGATGAGAATGTGCCTTCGGGAACCGTGAGACAGGTGCTGCATGG	5	0.125	No Hit
TGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTC	5	0.125	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGTAGATCGG	5	0.125	No Hit
TGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.025	0.0	0.0	0.0	0.0
10	0.075	0.0	0.0	0.0	0.0
11	0.175	0.0	0.0	0.0	0.0
12	0.325	0.0	0.0	0.0	0.0
13	0.4	0.0	0.0	0.0	0.0
14	0.6	0.0	0.0	0.0	0.0
15	0.8	0.0	0.0	0.0	0.0
16	0.95	0.0	0.0	0.0	0.0
17	1.3	0.0	0.0	0.0	0.0
18	1.4	0.0	0.0	0.0	0.0
19	1.75	0.0	0.0	0.0	0.0
20	1.925	0.0	0.0	0.0	0.0
21	2.125	0.0	0.0	0.0	0.0
22	2.25	0.0	0.0	0.0	0.0
23	2.45	0.0	0.0	0.0	0.0
24	2.8	0.0	0.0	0.0	0.0
25	3.1	0.0	0.0	0.0	0.0
26	3.525	0.0	0.0	0.0	0.0
27	3.85	0.0	0.0	0.0	0.0
28	4.3	0.0	0.0	0.0	0.0
29	4.75	0.0	0.0	0.0	0.0
30	5.075	0.0	0.0	0.0	0.0
31	5.475	0.0	0.0	0.0	0.0
32	6.125	0.0	0.0	0.0	0.0
33	6.6	0.0	0.0	0.0	0.0
34	7.1	0.0	0.0	0.0	0.0
35	7.575	0.0	0.0	0.0	0.0
36	8.15	0.0	0.0	0.0	0.0
37	8.725	0.0	0.0	0.0	0.0
38	9.275	0.0	0.0	0.0	0.0
39	9.575	0.0	0.0	0.0	0.0
40	10.325	0.0	0.0	0.0	0.0
41	11.25	0.0	0.0	0.0	0.0
42	11.95	0.0	0.0	0.0	0.0
43	12.525	0.0	0.0	0.0	0.0
44	14.475	0.0	0.0	0.0	0.0
45	15.525	0.0	0.0	0.0	0.0
46	16.275	0.0	0.0	0.0	0.0
47	17.2	0.0	0.0	0.0	0.0
48	18.325	0.0	0.0	0.0	0.0
49	19.7	0.0	0.0	0.0	0.0
50	20.825	0.0	0.0	0.0	0.0
51	21.5	0.0	0.0	0.0	0.0
52	22.225	0.0	0.0	0.0	0.0
53	23.575	0.0	0.0	0.0	0.0
54	24.425	0.0	0.0	0.0	0.0
55	25.45	0.0	0.0	0.0	0.0
56	25.95	0.0	0.0	0.0	0.0
57	26.425	0.0	0.0	0.0	0.0
58	26.925	0.0	0.0	0.0	0.0
59	27.8	0.0	0.0	0.0	0.0
60	28.7	0.0	0.0	0.0	0.0
61	29.4	0.0	0.0	0.0	0.0
62	29.9	0.0	0.0	0.0	0.0
63	30.4	0.0	0.0	0.0	0.0
64	31.35	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACAGG	20	1.1604347E-5	99.446434	70
AAGAGTT	50	6.566552E-10	55.69	7
TGATCAT	50	6.566552E-10	55.69	14
GAGTTTG	50	6.566552E-10	55.69	9
AGAGTTT	50	6.566552E-10	55.69	8
TTGATCA	50	6.566552E-10	55.69	13
GATCATG	50	6.566552E-10	55.69	15
AGTTTGA	50	6.566552E-10	55.69	10
TTTGATC	50	6.566552E-10	55.69	12
CGCTGGC	45	1.9927029E-8	54.143055	34
AACGCTG	45	1.9927029E-8	54.143055	32
ACGCTGG	45	1.9927029E-8	54.143055	33
ATGGCTC	45	1.9927029E-8	54.143055	19
GAACGCT	45	1.9927029E-8	54.143055	31
GATTGAA	45	1.9927029E-8	54.143055	27
TCAGATT	45	1.9927029E-8	54.143055	24
CAGATTG	45	1.9927029E-8	54.143055	25
ATTGAAC	45	1.9927029E-8	54.143055	28
TTGAACG	45	1.9927029E-8	54.143055	29
TGAACGC	45	1.9927029E-8	54.143055	30
>>END_MODULE
SRR32999257 read2 length is 69-76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32999257_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	69-76
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.3425	32.0	32.0	32.0	21.0	32.0
2	30.1255	32.0	32.0	32.0	21.0	32.0
3	30.118	32.0	32.0	32.0	21.0	32.0
4	30.0415	32.0	32.0	32.0	21.0	32.0
5	30.24375	32.0	32.0	32.0	21.0	32.0
6	33.18775	36.0	36.0	36.0	21.0	36.0
7	33.3625	36.0	36.0	36.0	21.0	36.0
8	33.313	36.0	36.0	36.0	21.0	36.0
9	33.28325	36.0	36.0	36.0	21.0	36.0
10-11	33.37175	36.0	36.0	36.0	21.0	36.0
12-13	33.24275	36.0	36.0	36.0	21.0	36.0
14-15	33.1335	36.0	36.0	36.0	17.5	36.0
16-17	33.236125	36.0	36.0	36.0	17.5	36.0
18-19	33.088499999999996	36.0	36.0	36.0	17.5	36.0
20-21	33.071875	36.0	36.0	36.0	17.5	36.0
22-23	33.05275	36.0	36.0	36.0	14.0	36.0
24-25	32.920500000000004	36.0	36.0	36.0	14.0	36.0
26-27	32.94075	36.0	36.0	36.0	14.0	36.0
28-29	33.160624999999996	36.0	36.0	36.0	17.5	36.0
30-31	32.749625	36.0	36.0	36.0	14.0	36.0
32-33	32.754000000000005	36.0	36.0	36.0	14.0	36.0
34-35	32.6535	36.0	36.0	36.0	14.0	36.0
36-37	32.567	36.0	36.0	36.0	14.0	36.0
38-39	32.3485	36.0	32.0	36.0	14.0	36.0
40-41	32.317125	36.0	32.0	36.0	14.0	36.0
42-43	32.22125	36.0	32.0	36.0	14.0	36.0
44-45	31.9445	36.0	32.0	36.0	14.0	36.0
46-47	32.060375	36.0	32.0	36.0	14.0	36.0
48-49	31.912374999999997	36.0	32.0	36.0	14.0	36.0
50-51	31.631500000000003	36.0	32.0	36.0	14.0	36.0
52-53	31.613750000000003	36.0	32.0	36.0	14.0	36.0
54-55	31.533125	36.0	32.0	36.0	14.0	36.0
56-57	31.31875	36.0	32.0	36.0	14.0	36.0
58-59	31.464750000000002	36.0	32.0	36.0	14.0	36.0
60-61	30.970374999999997	36.0	32.0	36.0	14.0	36.0
62-63	31.07575	36.0	32.0	36.0	14.0	36.0
64-65	30.858249999999998	36.0	29.5	36.0	14.0	36.0
66-67	30.861625	36.0	29.5	36.0	14.0	36.0
68-69	30.509999999999998	36.0	27.0	36.0	14.0	36.0
70-71	30.318989241931448	36.0	27.0	36.0	14.0	36.0
72-73	30.058963873580126	36.0	27.0	36.0	14.0	36.0
74-75	29.652591944628693	36.0	27.0	36.0	14.0	36.0
76	26.169605077574047	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10-11	0.0
11101	12-13	0.0
11101	14-15	0.0
11101	16-17	0.0
11101	18-19	0.0
11101	20-21	0.0
11101	22-23	0.0
11101	24-25	0.0
11101	26-27	0.0
11101	28-29	0.0
11101	30-31	0.0
11101	32-33	0.0
11101	34-35	0.0
11101	36-37	0.0
11101	38-39	0.0
11101	40-41	0.0
11101	42-43	0.0
11101	44-45	0.0
11101	46-47	0.0
11101	48-49	0.0
11101	50-51	0.0
11101	52-53	0.0
11101	54-55	0.0
11101	56-57	0.0
11101	58-59	0.0
11101	60-61	0.0
11101	62-63	0.0
11101	64-65	0.0
11101	66-67	0.0
11101	68-69	0.0
11101	70-71	0.0
11101	72-73	0.0
11101	74-75	0.0
11101	76	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	3.0
19	5.0
20	14.0
21	24.0
22	30.0
23	40.0
24	85.0
25	91.0
26	122.0
27	176.0
28	172.0
29	237.0
30	265.0
31	323.0
32	393.0
33	569.0
34	797.0
35	652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.15	27.0	13.55	17.299999999999997
2	18.9	23.7	16.525000000000002	40.875
3	15.825	18.7	29.4	36.075
4	21.099999999999998	21.375	26.150000000000002	31.374999999999996
5	19.6	17.424999999999997	26.924999999999997	36.05
6	17.05	21.875	23.5	37.574999999999996
7	19.75	24.125	22.375	33.75
8	21.0	19.825	27.700000000000003	31.474999999999998
9	19.925	17.525	25.775	36.775000000000006
10-11	19.1875	19.9375	26.1	34.775
12-13	20.4125	20.75	24.4125	34.425
14-15	20.474999999999998	23.45	24.375	31.7
16-17	20.5875	21.0625	28.125	30.225
18-19	23.2125	21.1375	23.65	32.0
20-21	19.55	22.25	25.1	33.1
22-23	18.9375	22.925	23.375	34.7625
24-25	21.825	21.4375	25.6125	31.125000000000004
26-27	20.575	22.1875	26.1	31.137500000000003
28-29	19.375	24.425	23.3	32.9
30-31	21.3	23.9	24.975	29.825000000000003
32-33	22.6375	21.3625	23.5625	32.4375
34-35	23.4625	23.1375	25.412499999999998	27.987499999999997
36-37	21.45	23.775	23.1625	31.612499999999997
38-39	23.3625	22.8375	23.2375	30.562499999999996
40-41	23.0875	22.112499999999997	27.35	27.450000000000003
42-43	21.2	20.8625	24.7875	33.15
44-45	22.162499999999998	23.6125	24.375	29.849999999999998
46-47	25.0	22.4375	24.4375	28.125
48-49	24.962500000000002	23.1625	23.5	28.375
50-51	25.35	23.3	24.1125	27.237499999999997
52-53	26.687499999999996	24.65	22.0	26.6625
54-55	26.700000000000003	22.8875	23.0875	27.325
56-57	26.9625	22.9625	23.0	27.075
58-59	26.9625	22.225	23.5125	27.3
60-61	27.750000000000004	23.2125	23.0875	25.95
62-63	26.637499999999996	22.5625	23.175	27.625
64-65	28.3625	23.2875	22.6125	25.7375
66-67	30.45	23.1375	22.6375	23.775
68-69	28.1375	25.5125	21.325	25.025
70-71	31.41105829372029	25.281461095821868	20.815611708781585	22.491868901676256
72-73	27.996993611424276	24.652386320931978	24.001002129525244	23.349617938118502
74-75	28.392810627767645	26.712685595207088	24.00364678301641	20.890856994008857
76	39.033850493653034	33.004231311706626	27.96191819464034	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.0
20	1.0
21	0.0
22	0.0
23	0.5
24	2.0
25	3.0
26	2.0
27	0.5
28	0.5
29	1.0
30	1.5
31	1.5
32	2.0
33	3.0
34	3.5
35	4.0
36	4.0
37	3.5
38	2.5
39	3.5
40	7.0
41	12.0
42	14.0
43	23.0
44	43.0
45	75.0
46	94.5
47	119.0
48	230.5
49	349.0
50	398.5
51	443.5
52	493.5
53	521.0
54	523.5
55	464.0
56	371.0
57	299.0
58	250.5
59	196.5
60	139.5
61	91.5
62	52.5
63	39.5
64	33.0
65	22.0
66	7.0
67	0.5
68	0.5
69	0.5
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.5
78	0.5
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	1.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	3.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
69	3.0
70	0.0
71	1.0
72	9.0
73	41.0
74	214.0
75	896.0
76	2836.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.15
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.06098406098405	60.650000000000006
2	8.316008316008316	12.0
3	3.3610533610533606	7.2749999999999995
4	1.4553014553014554	4.2
5	0.9009009009009009	3.25
6	0.6583506583506583	2.85
7	0.2772002772002772	1.4000000000000001
8	0.2772002772002772	1.6
9	0.2079002079002079	1.35
>10	0.4851004851004851	5.425
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAGATCGG	29	0.7250000000000001	No Hit
GATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTC	25	0.625	No Hit
GTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAAT	23	0.575	No Hit
GACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGT	21	0.525	No Hit
GCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAGA	17	0.42500000000000004	No Hit
GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGA	15	0.375	No Hit
GCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTC	13	0.325	No Hit
GAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACT	11	0.27499999999999997	No Hit
GTCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCA	11	0.27499999999999997	No Hit
AGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACA	11	0.27499999999999997	No Hit
GCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAG	11	0.27499999999999997	No Hit
GGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAG	10	0.25	No Hit
GACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCG	10	0.25	No Hit
AGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTA	10	0.25	No Hit
GCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAGATCG	9	0.22499999999999998	No Hit
GACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACC	9	0.22499999999999998	No Hit
ACGTCAATGAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTT	9	0.22499999999999998	No Hit
ACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTT	9	0.22499999999999998	No Hit
GCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGAT	9	0.22499999999999998	No Hit
GGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCC	9	0.22499999999999998	No Hit
AACGTCAATGAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAA	8	0.2	No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACT	8	0.2	No Hit
GCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAGATCGGAAG	8	0.2	No Hit
GCAGCACCTGTCTCACGGTTCCCGAAGGCACATTCTCATCTCTGAAAACT	8	0.2	No Hit
ACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTAC	8	0.2	No Hit
CCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATC	8	0.2	No Hit
GACTTGACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTT	8	0.2	No Hit
GTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCA	8	0.2	No Hit
ACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAG	7	0.17500000000000002	No Hit
GCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAG	7	0.17500000000000002	No Hit
AATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATT	7	0.17500000000000002	No Hit
GATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	7	0.17500000000000002	No Hit
GACGACAGCCATGCAGCACCTGTCTCACGGTTCCCGAAGGCACATTCTCA	7	0.17500000000000002	No Hit
GTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAACAGCAAGCTGTTTCCTGTTACCGTTCGACTTG	7	0.17500000000000002	No Hit
GGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCA	7	0.17500000000000002	No Hit
GACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTC	7	0.17500000000000002	No Hit
ACACGAGCTGACGACAGCCATGCAGCACCTGTCTCACGGTTCCCGAAGGC	6	0.15	No Hit
CGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTTC	6	0.15	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	6	0.15	No Hit
GTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGCCTG	6	0.15	No Hit
ACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCAC	6	0.15	No Hit
GGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCA	6	0.15	No Hit
AACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCT	6	0.15	No Hit
ACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGA	6	0.15	No Hit
AGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACA	6	0.15	No Hit
ACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTT	6	0.15	No Hit
ACCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAGATCGG	6	0.15	No Hit
GCCAGTATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGA	6	0.15	No Hit
GGTAACGTCAATGAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTG	6	0.15	No Hit
GTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCAAAGAAGCAAGCTT	6	0.15	No Hit
CCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCG	6	0.15	No Hit
CACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	6	0.15	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGC	6	0.15	No Hit
CCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTA	6	0.15	No Hit
GTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCA	6	0.15	No Hit
GACATTACTCACCCGTCCGCCACTCGTCAGCAAAGAAGCAAGCTTCTTCC	5	0.125	No Hit
AGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCC	5	0.125	No Hit
GTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGC	5	0.125	No Hit
AGTAACGTCAATGAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTG	5	0.125	No Hit
GTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATG	5	0.125	No Hit
GGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCT	5	0.125	No Hit
GAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCA	5	0.125	No Hit
GGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAA	5	0.125	No Hit
TAACGTCAATGAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTT	5	0.125	No Hit
AAGCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCG	5	0.125	No Hit
GCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTA	5	0.125	No Hit
ACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGA	5	0.125	No Hit
CCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTC	5	0.125	No Hit
ATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAA	5	0.125	No Hit
ATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGG	5	0.125	No Hit
AGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA	5	0.125	No Hit
GTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTG	5	0.125	No Hit
CCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCA	5	0.125	No Hit
GCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACAT	5	0.125	No Hit
GCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTT	5	0.125	No Hit
GTAACGTCAATGAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTT	5	0.125	No Hit
CCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATG	5	0.125	No Hit
ACCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCC	5	0.125	No Hit
CCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGA	5	0.125	No Hit
GCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCC	5	0.125	No Hit
ACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.025	0.0	0.0	0.0	0.0
10	0.075	0.0	0.0	0.0	0.0
11	0.2	0.0	0.0	0.0	0.0
12	0.35	0.0	0.0	0.0	0.0
13	0.4	0.0	0.0	0.0	0.0
14	0.6	0.0	0.0	0.0	0.0
15	0.75	0.0	0.0	0.0	0.0
16	0.875	0.0	0.0	0.0	0.0
17	1.225	0.0	0.0	0.0	0.0
18	1.3	0.0	0.0	0.0	0.0
19	1.625	0.0	0.0	0.0	0.0
20	1.825	0.0	0.0	0.0	0.0
21	2.025	0.0	0.0	0.0	0.0
22	2.175	0.0	0.0	0.0	0.0
23	2.35	0.0	0.0	0.0	0.0
24	2.65	0.0	0.0	0.0	0.0
25	2.95	0.0	0.0	0.0	0.0
26	3.325	0.0	0.0	0.0	0.0
27	3.65	0.0	0.0	0.0	0.0
28	4.1	0.0	0.0	0.0	0.0
29	4.55	0.0	0.0	0.0	0.0
30	5.0	0.0	0.0	0.0	0.0
31	5.375	0.0	0.0	0.0	0.0
32	5.95	0.0	0.0	0.0	0.0
33	6.425	0.0	0.0	0.0	0.0
34	6.875	0.0	0.0	0.0	0.0
35	7.3	0.0	0.0	0.0	0.0
36	7.875	0.0	0.0	0.0	0.0
37	8.5	0.0	0.0	0.0	0.0
38	8.925	0.0	0.0	0.0	0.0
39	9.3	0.0	0.0	0.0	0.0
40	10.025	0.0	0.0	0.0	0.0
41	10.875	0.0	0.0	0.0	0.0
42	11.575	0.0	0.0	0.0	0.0
43	12.075	0.0	0.0	0.0	0.0
44	13.95	0.0	0.0	0.0	0.0
45	15.15	0.0	0.0	0.0	0.0
46	15.775	0.0	0.0	0.0	0.0
47	16.625	0.0	0.0	0.0	0.0
48	17.65	0.0	0.0	0.0	0.0
49	18.85	0.0	0.0	0.0	0.0
50	20.05	0.0	0.0	0.0	0.0
51	20.65	0.0	0.0	0.0	0.0
52	21.4	0.0	0.0	0.0	0.0
53	22.675	0.0	0.0	0.0	0.0
54	23.525	0.0	0.0	0.0	0.0
55	24.525	0.0	0.0	0.0	0.0
56	25.05	0.0	0.0	0.0	0.0
57	25.6	0.0	0.0	0.0	0.0
58	26.1	0.0	0.0	0.0	0.0
59	26.95	0.0	0.0	0.0	0.0
60	27.8	0.0	0.0	0.0	0.0
61	28.525	0.0	0.0	0.0	0.0
62	29.0	0.0	0.0	0.0	0.0
63	29.55	0.0	0.0	0.0	0.0
64	30.5	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCACC	20	0.0065928185	52.228123	2
GCAAGCT	20	0.0065928185	52.228123	67
CCTGTCT	20	0.0065928185	52.228123	7
ACCTGTC	20	0.0065928185	52.228123	6
CACCTGT	20	0.0065928185	52.228123	5
AGCACCT	20	0.0065928185	52.228123	3
CTGTCTC	20	0.0065928185	52.228123	8
AGCAAGC	20	0.0065928185	52.228123	66
>>END_MODULE
Read 749792 spots for SRR32999257.sra
Written 749792 spots for SRR32999257.sra
Read 749799 spots for SRR32999257.sra
Written 749799 spots for SRR32999257.sra
Read 749792 spots for SRR32999257.sra
Written 749792 spots for SRR32999257.sra
Read 749792 spots for SRR32999257.sra
Written 749792 spots for SRR32999257.sra
Read 749792 spots for SRR32999257.sra
Written 749792 spots for SRR32999257.sra
Read 749792 spots for SRR32999257.sra
Written 749792 spots for SRR32999257.sra
Read 749792 spots for SRR32999257.sra
Written 749792 spots for SRR32999257.sra
Read 749792 spots for SRR32999257.sra
Written 749792 spots for SRR32999257.sra
SRR ids: ['SRR32999257.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_u1h4fs2j
SRR32999257.sra spots: 5998343
blocks: [[1, 749792], [749793, 1499584], [1499585, 2249376], [2249377, 2999168], [2999169, 3748960], [3748961, 4498752], [4498753, 5248544], [5248545, 5998343]]
SRR32999257 file size 1342748
SRR32999257 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32999257 SRR32999257_1.fastq SRR32999257_2.fastq
Input file:	SRR32999257_1.fastq
Paired file:	SRR32999257_2.fastq
trimmed:	SRR32999257-trimmed-pair1.fastq, SRR32999257-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu May 21 17:20:50 2026 >> started

Thu May 21 17:20:59 2026 >> done (9.210s)
5998343 read pairs processed; of these:
 202950 ( 3.38%) short read pairs filtered out after trimming by size control
    614 ( 0.01%) empty read pairs filtered out after trimming by size control
5794779 (96.61%) read pairs available; of these:
3281831 (56.63%) trimmed read pairs available after processing
2512948 (43.37%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  38034	  0.66%
 19	  34685	  0.60%
 20	  38438	  0.66%
 21	  41409	  0.71%
 22	  43950	  0.76%
 23	  40864	  0.71%
 24	  44869	  0.77%
 25	  48723	  0.84%
 26	  46492	  0.80%
 27	  51503	  0.89%
 28	  48069	  0.83%
 29	  53204	  0.92%
 30	  56170	  0.97%
 31	  66295	  1.14%
 32	  48827	  0.84%
 33	  61302	  1.06%
 34	  55115	  0.95%
 35	  53205	  0.92%
 36	  58344	  1.01%
 37	  65485	  1.13%
 38	  58453	  1.01%
 39	  61298	  1.06%
 40	  78339	  1.35%
 41	  58952	  1.02%
 42	  65356	  1.13%
 43	 134676	  2.32%
 44	  85629	  1.48%
 45	  65925	  1.14%
 46	  70738	  1.22%
 47	  89430	  1.54%
 48	 103847	  1.79%
 49	  89367	  1.54%
 50	  66014	  1.14%
 51	  70197	  1.21%
 52	  85503	  1.48%
 53	  59704	  1.03%
 54	  68269	  1.18%
 55	  49595	  0.86%
 56	  44311	  0.76%
 57	  46856	  0.81%
 58	  58964	  1.02%
 59	  56997	  0.98%
 60	  47976	  0.83%
 61	  46540	  0.80%
 62	  53752	  0.93%
 63	  57370	  0.99%
 64	  47573	  0.82%
 65	  42825	  0.74%
 66	  39383	  0.68%
 67	  40572	  0.70%
 68	  44096	  0.76%
 69	  48746	  0.84%
 70	  44431	  0.77%
 71	  40628	  0.70%
 72	  41376	  0.71%
 73	  51890	  0.90%
 74	 206342	  3.56%
 75	1245387	 21.49%
 76	1132489	 19.54%
5794779 reads passed initial QC


criterion=sequence-density
sequence-density=5.30
sequence-density-rank=1
fanout-score=2.06
fanout-score-rank=25
prefix-density=4.89
prefix-fanout=2.1
sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG


criterion=fanout-score
sequence-density=0.06
sequence-density-rank=25
fanout-score=160.98
fanout-score-rank=1
prefix-density=9.88
prefix-fanout=1.0
sequence=GGCCTAACACAAGCAAGTCGAAC


criterion=sequence-density
sequence-density=3.25
sequence-density-rank=1
fanout-score=2.24
fanout-score-rank=28
prefix-density=7.00
prefix-fanout=1.0
sequence=CACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAA


criterion=fanout-score
sequence-density=0.08
sequence-density-rank=33
fanout-score=110.88
fanout-score-rank=1
prefix-density=8.71
prefix-fanout=1.0
sequence=TGATCAAACTCATCAATTTAGAT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG -y CACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAA -o SRR32999257 SRR32999257_1.fastq SRR32999257_2.fastq
Input file:	SRR32999257_1.fastq
Paired file:	SRR32999257_2.fastq
trimmed:	SRR32999257-trimmed-pair1.fastq, SRR32999257-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACT
-- paired 3' end adapter sequence (-y):	CACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu May 21 17:21:20 2026 >> started

Thu May 21 17:21:26 2026 >> done (6.230s)
3476868 read pairs processed; of these:
   3490 ( 0.10%) short read pairs filtered out after trimming by size control
  21847 ( 0.63%) empty read pairs filtered out after trimming by size control
3451531 (99.27%) read pairs available; of these:
    337 ( 0.01%) trimmed read pairs available after processing
3451194 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  22859	  0.66%
 19	  20769	  0.60%
 20	  23036	  0.67%
 21	  24823	  0.72%
 22	  26110	  0.76%
 23	  24603	  0.71%
 24	  26827	  0.78%
 25	  29249	  0.85%
 26	  27824	  0.81%
 27	  30909	  0.90%
 28	  28941	  0.84%
 29	  31880	  0.92%
 30	  33382	  0.97%
 31	  39425	  1.14%
 32	  29089	  0.84%
 33	  36406	  1.05%
 34	  32997	  0.96%
 35	  31817	  0.92%
 36	  35037	  1.02%
 37	  38758	  1.12%
 38	  34921	  1.01%
 39	  36660	  1.06%
 40	  46621	  1.35%
 41	  35109	  1.02%
 42	  38961	  1.13%
 43	  80275	  2.33%
 44	  51122	  1.48%
 45	  39421	  1.14%
 46	  42358	  1.23%
 47	  53224	  1.54%
 48	  62229	  1.80%
 49	  53660	  1.55%
 50	  39270	  1.14%
 51	  41864	  1.21%
 52	  51209	  1.48%
 53	  35786	  1.04%
 54	  40725	  1.18%
 55	  29472	  0.85%
 56	  26592	  0.77%
 57	  28120	  0.81%
 58	  34846	  1.01%
 59	  33956	  0.98%
 60	  28476	  0.83%
 61	  27087	  0.78%
 62	  31747	  0.92%
 63	  34260	  0.99%
 64	  28039	  0.81%
 65	  25533	  0.74%
 66	  23615	  0.68%
 67	  24353	  0.71%
 68	  26274	  0.76%
 69	  29041	  0.84%
 70	  26377	  0.76%
 71	  24201	  0.70%
 72	  24692	  0.72%
 73	  31108	  0.90%
 74	 122925	  3.56%
 75	 740096	 21.44%
 76	 672565	 19.49%


criterion=sequence-density
sequence-density=5.10
sequence-density-rank=1
fanout-score=2.07
fanout-score-rank=23
prefix-density=4.92
prefix-fanout=2.1
sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG


criterion=fanout-score
sequence-density=1.05
sequence-density-rank=2
fanout-score=41.82
fanout-score-rank=1
prefix-density=1.26
prefix-fanout=35.0
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGATCTATCTCGTATGCCGT


criterion=sequence-density
sequence-density=3.26
sequence-density-rank=1
fanout-score=2.21
fanout-score-rank=29
prefix-density=6.89
prefix-fanout=1.0
sequence=CACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=41
fanout-score=900.27
fanout-score-rank=1
prefix-density=4.73
prefix-fanout=1.0
sequence=GTGTTAGGCCTACCGCCAGCGTT
SRR32999257 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 21 17:22:15
                             Started mapping on |	May 21 17:22:15
                                    Finished on |	May 21 17:26:42
       Mapping speed, Million of reads per hour |	77.79

                          Number of input reads |	5769442
                      Average input read length |	117
                                    UNIQUE READS:
                   Uniquely mapped reads number |	358811
                        Uniquely mapped reads % |	6.22%
                          Average mapped length |	127.25
                       Number of splices: Total |	34549
            Number of splices: Annotated (sjdb) |	8
                       Number of splices: GT/AG |	25874
                       Number of splices: GC/AG |	1043
                       Number of splices: AT/AC |	38
               Number of splices: Non-canonical |	7594
                      Mismatch rate per base, % |	0.83%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.19
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.20
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	5062954
             % of reads mapped to multiple loci |	87.75%
        Number of reads mapped to too many loci |	28762
             % of reads mapped to too many loci |	0.50%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.42%
                     % of reads unmapped: other |	2.10%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	347677	347677	347677
N_multimapping	5062954	5062954	5062954
N_noFeature	1033	7536	344492
N_ambiguous	16566	8915	2009
UnstrandedReadsAssigned:341212 PositiveStrandReadsAssigned:342360 NegativeStrandReadsAssigned:12310
Dataset is classified positive stranded
MeadianReadLen=66 20thPercentileLength=40 echo kmer=35
SRR32999257 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR32999257-trimmed-pair1.fastq
                             SRR32999257-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 5,769,442 reads, 1,174 reads pseudoaligned
[quant] estimated average fragment length: 68.0619
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 120 rounds

  4323 SRR32999257.ke.tsv
  4497 SRR32999257.se.tsv
  8820 total
==> SRR32999257.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	19.4181	0	0
AAC73113	2463	2395.94	0	0
AAC73114	933	865.938	2	409.823
AAC73115	1287	1219.94	0	0
AAC73116	297	230.177	0	0
AAC73117	777	709.938	0	0
AAC73118	1431	1363.94	0	0
AAC73119	954	886.938	2	400.119
AAC73120	588	520.938	0	0

==> SRR32999257.se.tsv <==
b0001	0
b0002	0
b0003	2
b0004	0
b0005	0
b0006	0
b0007	0
b0008	3
b0009	0
b0010	1
SRR32999257 completed mapping pipeline successfully
