Starting /dee2/code/volunteer_pipeline.sh SRR32999268
    current disk space = 238383712
    free memory = 113796064 
SRR32999268 SRAfilesize
c5a3e8c55f0b66ed40a7b03e34efc073  SRR32999268.sra
SRR32999268.sra file validated
SRR32999268 is paired end
SRR32999268 is conventional basespace
SRR32999268 read1 length is 71-76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32999268_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	71-76
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.2265	32.0	32.0	32.0	21.0	32.0
2	30.733	32.0	32.0	32.0	32.0	32.0
3	30.66275	32.0	32.0	32.0	32.0	32.0
4	30.82175	32.0	32.0	32.0	32.0	32.0
5	30.75525	32.0	32.0	32.0	32.0	32.0
6	33.59	36.0	36.0	36.0	32.0	36.0
7	33.95125	36.0	36.0	36.0	32.0	36.0
8	33.6355	36.0	36.0	36.0	32.0	36.0
9	33.922	36.0	36.0	36.0	32.0	36.0
10-11	33.96425	36.0	36.0	36.0	32.0	36.0
12-13	33.9415	36.0	36.0	36.0	32.0	36.0
14-15	34.07125	36.0	36.0	36.0	32.0	36.0
16-17	33.93	36.0	36.0	36.0	32.0	36.0
18-19	33.803	36.0	36.0	36.0	32.0	36.0
20-21	33.8485	36.0	36.0	36.0	32.0	36.0
22-23	33.83175	36.0	36.0	36.0	32.0	36.0
24-25	33.807375	36.0	36.0	36.0	29.5	36.0
26-27	33.779875	36.0	36.0	36.0	32.0	36.0
28-29	33.557249999999996	36.0	36.0	36.0	26.5	36.0
30-31	33.647125	36.0	36.0	36.0	29.5	36.0
32-33	33.205375000000004	36.0	36.0	36.0	17.5	36.0
34-35	33.47225	36.0	36.0	36.0	24.0	36.0
36-37	33.4855	36.0	36.0	36.0	27.0	36.0
38-39	33.38525	36.0	36.0	36.0	24.0	36.0
40-41	33.411125	36.0	36.0	36.0	21.0	36.0
42-43	33.35575	36.0	36.0	36.0	21.0	36.0
44-45	33.258624999999995	36.0	36.0	36.0	21.0	36.0
46-47	33.0155	36.0	36.0	36.0	14.0	36.0
48-49	33.048125	36.0	36.0	36.0	21.0	36.0
50-51	32.6135	36.0	32.0	36.0	14.0	36.0
52-53	32.72625	36.0	32.0	36.0	17.5	36.0
54-55	32.8705	36.0	32.0	36.0	21.0	36.0
56-57	32.736999999999995	36.0	32.0	36.0	17.5	36.0
58-59	32.556875000000005	36.0	32.0	36.0	14.0	36.0
60-61	32.509	36.0	32.0	36.0	14.0	36.0
62-63	32.52575	36.0	32.0	36.0	14.0	36.0
64-65	32.33075	36.0	32.0	36.0	14.0	36.0
66-67	32.171375	36.0	32.0	36.0	14.0	36.0
68-69	32.285	36.0	32.0	36.0	14.0	36.0
70-71	31.92125	36.0	32.0	36.0	14.0	36.0
72-73	32.141910477619405	36.0	32.0	36.0	14.0	36.0
74-75	32.082098693424456	36.0	32.0	36.0	14.0	36.0
76	29.793451758969	36.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10-11	0.0
11101	12-13	0.0
11101	14-15	0.0
11101	16-17	0.0
11101	18-19	0.0
11101	20-21	0.0
11101	22-23	0.0
11101	24-25	0.0
11101	26-27	0.0
11101	28-29	0.0
11101	30-31	0.0
11101	32-33	0.0
11101	34-35	0.0
11101	36-37	0.0
11101	38-39	0.0
11101	40-41	0.0
11101	42-43	0.0
11101	44-45	0.0
11101	46-47	0.0
11101	48-49	0.0
11101	50-51	0.0
11101	52-53	0.0
11101	54-55	0.0
11101	56-57	0.0
11101	58-59	0.0
11101	60-61	0.0
11101	62-63	0.0
11101	64-65	0.0
11101	66-67	0.0
11101	68-69	0.0
11101	70-71	0.0
11101	72-73	0.0
11101	74-75	0.0
11101	76	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	3.0
23	8.0
24	18.0
25	41.0
26	77.0
27	115.0
28	146.0
29	193.0
30	267.0
31	283.0
32	376.0
33	524.0
34	846.0
35	1101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.0	35.825	28.999999999999996	20.175
2	26.125	40.9	19.3	13.675
3	30.2	39.875	16.7	13.225000000000001
4	26.05	29.4	28.575	15.975
5	25.624999999999996	27.625	32.1	14.649999999999999
6	45.725	19.5	18.55	16.225
7	35.075	34.599999999999994	14.924999999999999	15.4
8	28.749999999999996	42.85	13.925	14.475
9	45.775	24.15	15.85	14.224999999999998
10-11	37.8375	30.7125	14.674999999999999	16.775000000000002
12-13	32.45	17.8875	32.0625	17.599999999999998
14-15	35.6625	20.0875	25.937500000000004	18.3125
16-17	30.725	29.212500000000002	22.9625	17.1
18-19	26.687499999999996	27.650000000000002	19.2375	26.424999999999997
20-21	35.462500000000006	22.9375	24.337500000000002	17.2625
22-23	40.300000000000004	20.9375	16.575	22.1875
24-25	28.299999999999997	20.925	26.8375	23.9375
26-27	34.9875	30.025000000000002	16.925	18.0625
28-29	28.425	32.887499999999996	21.712500000000002	16.975
30-31	31.4625	22.825	28.15	17.5625
32-33	29.575000000000003	36.162499999999994	17.025000000000002	17.2375
34-35	37.012499999999996	23.8125	14.7375	24.4375
36-37	28.537499999999998	21.0375	21.425	28.999999999999996
38-39	41.625	20.974999999999998	18.525	18.875
40-41	34.675	22.175	15.837499999999999	27.3125
42-43	38.487500000000004	21.725	15.287500000000001	24.5
44-45	33.0875	30.337500000000002	15.187500000000002	21.3875
46-47	36.4625	24.925	14.975	23.6375
48-49	27.450000000000003	23.3875	20.4375	28.725
50-51	27.8875	36.425000000000004	16.275000000000002	19.412499999999998
52-53	28.275	30.612499999999997	15.625	25.4875
54-55	28.287499999999998	30.6875	15.262500000000001	25.7625
56-57	30.15	28.95	20.6875	20.2125
58-59	27.287499999999998	29.175	15.687499999999998	27.85
60-61	30.65	32.3125	15.85	21.1875
62-63	26.3625	24.0	23.4875	26.150000000000002
64-65	30.975	27.800000000000004	18.25	22.975
66-67	24.425	32.625	17.1	25.85
68-69	34.325	24.375	16.9625	24.337500000000002
70-71	26.9625	28.499999999999996	22.787499999999998	21.75
72-73	25.156289072268066	31.520380095023754	17.57939484871218	25.743935983995996
74-75	29.43955899724373	30.20081375508597	18.78199238745242	21.577634860217877
76	45.907349355625215	38.83664228491815	15.256008359456635	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.5
37	1.5
38	1.0
39	0.0
40	0.0
41	1.0
42	3.0
43	13.5
44	25.5
45	58.0
46	96.0
47	198.0
48	433.0
49	478.5
50	399.0
51	408.5
52	425.5
53	471.5
54	473.5
55	428.5
56	367.5
57	293.0
58	238.0
59	166.0
60	89.0
61	60.5
62	50.5
63	54.5
64	46.5
65	18.5
66	3.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	3.0
94	4.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
71	1.0
72	0.0
73	102.0
74	175.0
75	851.0
76	2871.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.58627528758429	52.05
2	8.964696548988496	11.3
3	3.2923443078143593	6.225
4	1.7453391511305039	4.3999999999999995
5	1.190003966679889	3.75
6	0.7933359777865925	3.0
7	0.43633478778262597	1.925
8	0.19833399444664812	1.0
9	0.15866719555731854	0.8999999999999999
>10	0.5553351844506148	5.25
>50	0.039666798889329634	2.475
>100	0.039666798889329634	7.725
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTA	309	7.725	No Hit
TAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCT	99	2.475	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGATCGG	33	0.8250000000000001	No Hit
ATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCA	26	0.65	No Hit
TGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGG	20	0.5	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCAGA	15	0.375	No Hit
CTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAA	14	0.35000000000000003	No Hit
TGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGG	14	0.35000000000000003	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCAGATCGGAAGAGCACACGTCTGAACTC	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACC	13	0.325	No Hit
AGAGATGAGAATGTGCCTTCGGGAACCGTGAGACAGGTGCTGCATGGCTG	11	0.27499999999999997	No Hit
TAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTA	10	0.25	No Hit
CTGGACGAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGAT	10	0.25	No Hit
TGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTAC	10	0.25	No Hit
AGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGT	10	0.25	No Hit
TGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGAC	9	0.22499999999999998	No Hit
AATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTC	9	0.22499999999999998	No Hit
TGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATA	9	0.22499999999999998	No Hit
TGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTC	9	0.22499999999999998	No Hit
TCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAA	8	0.2	No Hit
AGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGT	8	0.2	No Hit
TAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGG	8	0.2	No Hit
CAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAG	8	0.2	No Hit
GTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGA	8	0.2	No Hit
AACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCG	7	0.17500000000000002	No Hit
GGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAG	7	0.17500000000000002	No Hit
TGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACAC	7	0.17500000000000002	No Hit
GAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGA	7	0.17500000000000002	No Hit
CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG	7	0.17500000000000002	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCAGATCGGAAGAG	7	0.17500000000000002	No Hit
ATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGG	7	0.17500000000000002	No Hit
TAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG	7	0.17500000000000002	No Hit
CGTAAACGATGTCGACTTGGAGGTTGTGCCCTTGAGGCGTGGCTTCCGGA	7	0.17500000000000002	No Hit
CGGAAGTTTTCAGAGATGAGAATGTGCCTTCGGGAACCGTGAGACAGGTG	7	0.17500000000000002	No Hit
AAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGT	7	0.17500000000000002	No Hit
TCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGA	6	0.15	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACG	6	0.15	No Hit
GAGAGCAAGCGGACCTCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGC	6	0.15	No Hit
CAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTA	6	0.15	No Hit
AAAATGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTA	6	0.15	No Hit
AACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGG	6	0.15	No Hit
CGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCT	6	0.15	No Hit
TGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGG	6	0.15	No Hit
ATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCG	6	0.15	No Hit
TACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCTC	6	0.15	No Hit
TCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGG	6	0.15	No Hit
ATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAG	6	0.15	No Hit
TGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCC	6	0.15	No Hit
TTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACA	6	0.15	No Hit
TACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGG	6	0.15	No Hit
AGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACG	6	0.15	No Hit
CGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGG	6	0.15	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGTAGATCGG	6	0.15	No Hit
AAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCT	6	0.15	No Hit
CAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAA	6	0.15	No Hit
AGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCAC	5	0.125	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCAGATCGGAAG	5	0.125	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTAGATCGGAAGAGCACACGTCTGAACTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGACGAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATT	5	0.125	No Hit
TTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAA	5	0.125	No Hit
AGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGT	5	0.125	No Hit
CAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAG	5	0.125	No Hit
GCGAAGAACCTTACCTGGTCTTGACATCCACGGAAGTTTTCAGAGATGAG	5	0.125	No Hit
CGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA	5	0.125	No Hit
ACGGAAGTTTTCAGAGATGAGAATGTGCCTTCGGGAACCGTGAGACAGGT	5	0.125	No Hit
TAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGAGA	5	0.125	No Hit
TAAAATGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCT	5	0.125	No Hit
ATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCTCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAA	5	0.125	No Hit
CGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGG	5	0.125	No Hit
CTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGG	5	0.125	No Hit
GACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGG	5	0.125	No Hit
GATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACG	5	0.125	No Hit
ACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGG	5	0.125	No Hit
ACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	5	0.125	No Hit
TTGACATCCACGGAAGTTTTCAGAGATGAGAATGTGCCTTCGGGAACCGTGAGACAGGTGCTGCATGGCTGTCGT	5	0.125	No Hit
TTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACT	5	0.125	No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA	5	0.125	No Hit
AAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAA	5	0.125	No Hit
AAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAA	5	0.125	No Hit
TTGAGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGT	5	0.125	No Hit
AAATTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	5	0.125	No Hit
CATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCG	5	0.125	No Hit
GGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCC	5	0.125	No Hit
TGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCA	5	0.125	No Hit
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.025	0.0	0.0	0.0	0.0
11	0.1	0.0	0.0	0.0	0.0
12	0.2	0.0	0.0	0.0	0.0
13	0.275	0.0	0.0	0.0	0.0
14	0.525	0.0	0.0	0.0	0.0
15	0.75	0.0	0.0	0.0	0.0
16	0.95	0.0	0.0	0.0	0.0
17	1.2	0.0	0.0	0.0	0.0
18	1.425	0.0	0.0	0.0	0.0
19	1.65	0.0	0.0	0.0	0.0
20	1.925	0.0	0.0	0.0	0.0
21	2.275	0.0	0.0	0.0	0.0
22	2.425	0.0	0.0	0.0	0.0
23	2.85	0.0	0.0	0.0	0.0
24	3.2	0.0	0.0	0.0	0.0
25	3.65	0.0	0.0	0.0	0.0
26	4.05	0.0	0.0	0.0	0.0
27	4.45	0.0	0.0	0.0	0.0
28	4.85	0.0	0.0	0.0	0.0
29	5.375	0.0	0.0	0.0	0.0
30	5.75	0.0	0.0	0.0	0.0
31	6.05	0.0	0.0	0.0	0.0
32	6.625	0.0	0.0	0.0	0.0
33	7.125	0.0	0.0	0.0	0.0
34	7.725	0.0	0.0	0.0	0.0
35	8.35	0.0	0.0	0.0	0.0
36	8.825	0.0	0.0	0.0	0.0
37	9.225	0.0	0.0	0.0	0.0
38	9.825	0.0	0.0	0.0	0.0
39	10.575	0.0	0.0	0.0	0.0
40	11.175	0.0	0.0	0.0	0.0
41	11.825	0.0	0.0	0.0	0.0
42	12.4	0.0	0.0	0.0	0.0
43	13.075	0.0	0.0	0.0	0.0
44	14.775	0.0	0.0	0.0	0.0
45	15.65	0.0	0.0	0.0	0.0
46	16.575	0.0	0.0	0.0	0.0
47	17.425	0.0	0.0	0.0	0.0
48	18.8	0.0	0.0	0.0	0.0
49	20.3	0.0	0.0	0.0	0.0
50	21.325	0.0	0.0	0.0	0.0
51	22.2	0.0	0.0	0.0	0.0
52	22.95	0.0	0.0	0.0	0.0
53	24.25	0.0	0.0	0.0	0.0
54	24.95	0.0	0.0	0.0	0.0
55	25.775	0.0	0.0	0.0	0.0
56	26.35	0.0	0.0	0.0	0.0
57	26.8	0.0	0.0	0.0	0.0
58	27.4	0.0	0.0	0.0	0.0
59	28.35	0.0	0.0	0.0	0.0
60	29.25	0.0	0.0	0.0	0.0
61	29.85	0.0	0.0	0.0	0.0
62	30.675	0.0	0.0	0.0	0.0
63	31.475	0.0	0.0	0.0	0.0
64	32.075	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACAGG	35	1.7787897E-6	72.31168	70
TAAATTG	20	7.205523E-5	69.6	1
AACGCTG	75	0.0	55.679996	32
ACGCTGG	75	0.0	55.679996	33
GAACGCT	75	0.0	55.679996	31
GATTGAA	75	0.0	55.679996	27
ATTGAAC	75	0.0	55.679996	28
TTGAACG	75	0.0	55.679996	29
TGAACGC	75	0.0	55.679996	30
ATGGCTC	70	0.0	54.68571	19
GGCTCAG	70	0.0	54.68571	21
GCTCAGA	70	0.0	54.68571	22
TGGCTCA	70	0.0	54.68571	20
CGCTGGC	80	0.0	52.2	34
GTTTGAT	80	0.0	52.2	11
AAGAGTT	80	0.0	52.2	7
GCTGGCG	80	0.0	52.2	35
TGATCAT	80	0.0	52.2	14
GAAGAGT	80	0.0	52.2	6
GAGTTTG	80	0.0	52.2	9
>>END_MODULE
SRR32999268 read2 length is 70-76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32999268_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	70-76
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.25775	32.0	32.0	32.0	21.0	32.0
2	30.0825	32.0	32.0	32.0	21.0	32.0
3	30.07625	32.0	32.0	32.0	21.0	32.0
4	30.09675	32.0	32.0	32.0	21.0	32.0
5	30.107	32.0	32.0	32.0	21.0	32.0
6	33.3115	36.0	36.0	36.0	21.0	36.0
7	33.3345	36.0	36.0	36.0	21.0	36.0
8	33.216	36.0	36.0	36.0	21.0	36.0
9	33.244	36.0	36.0	36.0	21.0	36.0
10-11	33.341375	36.0	36.0	36.0	21.0	36.0
12-13	33.233000000000004	36.0	36.0	36.0	21.0	36.0
14-15	33.126625000000004	36.0	36.0	36.0	14.0	36.0
16-17	32.936875	36.0	36.0	36.0	14.0	36.0
18-19	33.073750000000004	36.0	36.0	36.0	14.0	36.0
20-21	33.066125	36.0	36.0	36.0	17.5	36.0
22-23	32.887	36.0	36.0	36.0	14.0	36.0
24-25	32.91475	36.0	36.0	36.0	14.0	36.0
26-27	33.014375	36.0	36.0	36.0	14.0	36.0
28-29	32.962374999999994	36.0	36.0	36.0	14.0	36.0
30-31	32.747	36.0	36.0	36.0	14.0	36.0
32-33	32.786375	36.0	36.0	36.0	14.0	36.0
34-35	32.491749999999996	36.0	36.0	36.0	14.0	36.0
36-37	32.555375	36.0	36.0	36.0	14.0	36.0
38-39	32.366625	36.0	32.0	36.0	14.0	36.0
40-41	32.216499999999996	36.0	32.0	36.0	14.0	36.0
42-43	32.024375	36.0	32.0	36.0	14.0	36.0
44-45	31.814124999999997	36.0	32.0	36.0	14.0	36.0
46-47	31.960375	36.0	32.0	36.0	14.0	36.0
48-49	31.803874999999998	36.0	32.0	36.0	14.0	36.0
50-51	31.81325	36.0	32.0	36.0	14.0	36.0
52-53	31.687375	36.0	32.0	36.0	14.0	36.0
54-55	31.327375	36.0	32.0	36.0	14.0	36.0
56-57	31.291	36.0	32.0	36.0	14.0	36.0
58-59	31.405	36.0	32.0	36.0	14.0	36.0
60-61	31.042875000000002	36.0	32.0	36.0	14.0	36.0
62-63	31.01725	36.0	32.0	36.0	14.0	36.0
64-65	30.896250000000002	36.0	29.5	36.0	14.0	36.0
66-67	30.721125	36.0	29.5	36.0	14.0	36.0
68-69	30.596375000000002	36.0	27.0	36.0	14.0	36.0
70-71	30.205022568142034	36.0	27.0	36.0	14.0	36.0
72-73	29.956871211702676	36.0	27.0	36.0	14.0	36.0
74-75	29.799419107565015	36.0	27.0	36.0	14.0	36.0
76	26.148254767902124	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10-11	0.0
11101	12-13	0.0
11101	14-15	0.0
11101	16-17	0.0
11101	18-19	0.0
11101	20-21	0.0
11101	22-23	0.0
11101	24-25	0.0
11101	26-27	0.0
11101	28-29	0.0
11101	30-31	0.0
11101	32-33	0.0
11101	34-35	0.0
11101	36-37	0.0
11101	38-39	0.0
11101	40-41	0.0
11101	42-43	0.0
11101	44-45	0.0
11101	46-47	0.0
11101	48-49	0.0
11101	50-51	0.0
11101	52-53	0.0
11101	54-55	0.0
11101	56-57	0.0
11101	58-59	0.0
11101	60-61	0.0
11101	62-63	0.0
11101	64-65	0.0
11101	66-67	0.0
11101	68-69	0.0
11101	70-71	0.0
11101	72-73	0.0
11101	74-75	0.0
11101	76	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	3.0
19	7.0
20	14.0
21	26.0
22	39.0
23	56.0
24	53.0
25	100.0
26	118.0
27	142.0
28	205.0
29	246.0
30	280.0
31	348.0
32	386.0
33	555.0
34	770.0
35	651.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.875	29.049999999999997	14.875	18.2
2	19.975	22.55	15.0	42.475
3	15.625	19.275000000000002	28.749999999999996	36.35
4	21.85	21.65	24.85	31.65
5	20.325	17.525	26.424999999999997	35.725
6	17.675	20.925	25.35	36.05
7	20.599999999999998	24.5	23.0	31.900000000000002
8	20.849999999999998	19.25	29.099999999999998	30.8
9	20.45	17.875	26.125	35.55
10-11	19.412499999999998	21.0375	25.025	34.525
12-13	19.575	20.625	24.975	34.825
14-15	20.9125	23.45	24.337500000000002	31.3
16-17	20.2375	20.175	28.1625	31.424999999999997
18-19	22.537499999999998	21.212500000000002	24.962500000000002	31.2875
20-21	20.849999999999998	22.7	23.8875	32.5625
22-23	20.6125	22.2625	23.75	33.375
24-25	21.275	22.3	25.337500000000002	31.087500000000002
26-27	20.875	22.7375	25.275	31.112499999999997
28-29	20.5375	24.337500000000002	22.7375	32.3875
30-31	21.837500000000002	24.125	24.875	29.1625
32-33	22.8125	21.1875	23.6125	32.3875
34-35	22.675	24.4375	24.9375	27.950000000000003
36-37	21.65	22.7	24.675	30.975
38-39	24.6625	21.975	23.0125	30.349999999999998
40-41	23.7125	22.237499999999997	27.474999999999998	26.575
42-43	21.9	20.5875	24.8625	32.65
44-45	23.525	23.2625	25.275	27.9375
46-47	24.349999999999998	22.6875	24.7875	28.175
48-49	24.725	22.3125	24.224999999999998	28.7375
50-51	25.5375	24.087500000000002	23.474999999999998	26.900000000000002
52-53	27.287499999999998	23.599999999999998	23.075000000000003	26.0375
54-55	26.85	22.162499999999998	24.95	26.0375
56-57	26.25	22.537499999999998	23.7125	27.500000000000004
58-59	26.487500000000004	23.2875	24.2	26.025
60-61	28.287499999999998	22.2625	23.45	26.0
62-63	26.174999999999997	22.900000000000002	23.5375	27.3875
64-65	27.3625	22.35	24.6125	25.674999999999997
66-67	29.612500000000004	22.325	23.125	24.9375
68-69	28.349999999999998	24.474999999999998	22.7625	24.4125
70-71	30.728841105138144	23.76547068383548	22.327790973871732	23.177897237154642
72-73	28.340030135610245	23.945253641386238	24.246609743847316	23.468106479156205
74-75	29.675384413194898	24.72072545669602	24.20817453016165	21.395715599947433
76	38.682979489024824	33.609211946743436	27.70780856423174	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	1.0
18	0.5
19	0.5
20	0.5
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	1.5
27	1.5
28	1.5
29	3.0
30	4.5
31	4.5
32	2.5
33	2.0
34	2.0
35	2.5
36	3.5
37	5.0
38	8.5
39	9.5
40	7.5
41	9.0
42	15.0
43	29.5
44	59.0
45	85.0
46	88.5
47	119.0
48	236.5
49	352.0
50	388.5
51	432.5
52	466.5
53	489.0
54	509.5
55	472.5
56	392.0
57	297.5
58	244.0
59	198.5
60	137.5
61	83.5
62	55.5
63	42.0
64	23.0
65	12.5
66	3.5
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	1.0
99	6.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
70	1.0
71	1.0
72	32.0
73	52.0
74	219.0
75	916.0
76	2779.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.07470288624788	61.9
2	8.35314091680815	12.3
3	3.429541595925297	7.575
4	1.629881154499151	4.8
5	0.9507640067911713	3.5000000000000004
6	0.5432937181663837	2.4
7	0.3395585738539898	1.7500000000000002
8	0.1697792869269949	1.0
9	0.10186757215619693	0.675
>10	0.40747028862478774	4.1000000000000005
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAGATCGG	34	0.8500000000000001	No Hit
GCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAG	14	0.35000000000000003	No Hit
GCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAGA	13	0.325	No Hit
GACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCG	13	0.325	No Hit
GAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACT	12	0.3	No Hit
GGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAG	12	0.3	No Hit
GCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAGATCG	12	0.3	No Hit
GACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACC	11	0.27499999999999997	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	11	0.27499999999999997	No Hit
CCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATC	11	0.27499999999999997	No Hit
GTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAAT	11	0.27499999999999997	No Hit
AGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACA	10	0.25	No Hit
GACATTACTCACCCGTCCGCCACTCGTCAGCAAAGAAGCAAGCTTCTTCC	9	0.22499999999999998	No Hit
GCAGCACCTGTCTCACGGTTCCCGAAGGCACATTCTCATCTCTGAAAACT	9	0.22499999999999998	No Hit
ACGTCAATGAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTT	9	0.22499999999999998	No Hit
GCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTC	8	0.2	No Hit
AATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCA	8	0.2	No Hit
GATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTC	8	0.2	No Hit
CACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	8	0.2	No Hit
ACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCT	8	0.2	No Hit
GAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCA	7	0.17500000000000002	No Hit
GACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCACGTGTGTAGC	7	0.17500000000000002	No Hit
CCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAA	7	0.17500000000000002	No Hit
GACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGT	7	0.17500000000000002	No Hit
GTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCC	7	0.17500000000000002	No Hit
ACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGA	7	0.17500000000000002	No Hit
ACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTAC	7	0.17500000000000002	No Hit
GGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGG	7	0.17500000000000002	No Hit
CTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCA	7	0.17500000000000002	No Hit
GTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCA	7	0.17500000000000002	No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT	6	0.15	No Hit
AGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAG	6	0.15	No Hit
GACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCC	6	0.15	No Hit
GTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACC	6	0.15	No Hit
GTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGCCTG	6	0.15	No Hit
ACTTGACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGGACCGCTGGCAA	6	0.15	No Hit
GTCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCA	6	0.15	No Hit
ACCCGTCCGCCACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCG	6	0.15	No Hit
GTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTG	6	0.15	No Hit
GACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAGATCGGAAGAG	6	0.15	No Hit
GCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTT	6	0.15	No Hit
ACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGG	6	0.15	No Hit
ACCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAGATCGG	6	0.15	No Hit
GAAGCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGAT	6	0.15	No Hit
GTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCAAAGAAGCAAGCTT	6	0.15	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGC	6	0.15	No Hit
GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGA	5	0.125	No Hit
GTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCAAAGAAGCAAG	5	0.125	No Hit
GTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGC	5	0.125	No Hit
GGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTG	5	0.125	No Hit
CGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTTC	5	0.125	No Hit
AGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCT	5	0.125	No Hit
GACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACC	5	0.125	No Hit
CCATGCAGCACCTGTCTCACGGTTCCCGAAGGCACATTCTCATCTCTGAA	5	0.125	No Hit
ATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCG	5	0.125	No Hit
ACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGA	5	0.125	No Hit
ACATTACTCACCCGTCCGCCACTCGTCAGCGAAACAGCAAGCTGTTTCCT	5	0.125	No Hit
CCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAGA	5	0.125	No Hit
ACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTT	5	0.125	No Hit
GTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCC	5	0.125	No Hit
CCAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGC	5	0.125	No Hit
GCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACT	5	0.125	No Hit
ATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTC	5	0.125	No Hit
CCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCA	5	0.125	No Hit
ACTCACCCGTCCGCCACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGC	5	0.125	No Hit
GATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATG	5	0.125	No Hit
GACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCA	5	0.125	No Hit
ACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAA	5	0.125	No Hit
CCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATG	5	0.125	No Hit
ACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGC	5	0.125	No Hit
ACCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCC	5	0.125	No Hit
AATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTT	5	0.125	No Hit
CCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGT	5	0.125	No Hit
GTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.025	0.0	0.0	0.0	0.0
11	0.075	0.0	0.0	0.0	0.0
12	0.175	0.0	0.0	0.0	0.0
13	0.25	0.0	0.0	0.0	0.0
14	0.475	0.0	0.0	0.0	0.0
15	0.7	0.0	0.0	0.0	0.0
16	0.925	0.0	0.0	0.0	0.0
17	1.175	0.0	0.0	0.0	0.0
18	1.35	0.0	0.0	0.0	0.0
19	1.55	0.0	0.0	0.0	0.0
20	1.775	0.0	0.0	0.0	0.0
21	2.1	0.0	0.0	0.0	0.0
22	2.35	0.0	0.0	0.0	0.0
23	2.75	0.0	0.0	0.0	0.0
24	3.05	0.0	0.0	0.0	0.0
25	3.425	0.0	0.0	0.0	0.0
26	3.7	0.0	0.0	0.0	0.0
27	4.1	0.0	0.0	0.0	0.0
28	4.475	0.0	0.0	0.0	0.0
29	4.975	0.0	0.0	0.0	0.0
30	5.325	0.0	0.0	0.0	0.0
31	5.675	0.0	0.0	0.0	0.0
32	6.25	0.0	0.0	0.0	0.0
33	6.825	0.0	0.0	0.0	0.0
34	7.45	0.0	0.0	0.0	0.0
35	8.075	0.0	0.0	0.0	0.0
36	8.575	0.0	0.0	0.0	0.0
37	9.0	0.0	0.0	0.0	0.0
38	9.575	0.0	0.0	0.0	0.0
39	10.3	0.0	0.0	0.0	0.0
40	10.875	0.0	0.0	0.0	0.0
41	11.475	0.0	0.0	0.0	0.0
42	12.025	0.0	0.0	0.0	0.0
43	12.725	0.0	0.0	0.0	0.0
44	14.35	0.0	0.0	0.0	0.0
45	15.3	0.0	0.0	0.0	0.0
46	16.2	0.0	0.0	0.0	0.0
47	17.05	0.0	0.0	0.0	0.0
48	18.375	0.0	0.0	0.0	0.0
49	19.95	0.0	0.0	0.0	0.0
50	20.875	0.0	0.0	0.0	0.0
51	21.65	0.0	0.0	0.0	0.0
52	22.4	0.0	0.0	0.0	0.0
53	23.725	0.0	0.0	0.0	0.0
54	24.4	0.0	0.0	0.0	0.0
55	25.325	0.0	0.0	0.0	0.0
56	25.85	0.0	0.0	0.0	0.0
57	26.35	0.0	0.0	0.0	0.0
58	26.95	0.0	0.0	0.0	0.0
59	27.85	0.0	0.0	0.0	0.0
60	28.75	0.0	0.0	0.0	0.0
61	29.4	0.0	0.0	0.0	0.0
62	30.15	0.0	0.0	0.0	0.0
63	30.875	0.0	0.0	0.0	0.0
64	31.45	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 601800 spots for SRR32999268.sra
Written 601800 spots for SRR32999268.sra
Read 601800 spots for SRR32999268.sra
Written 601800 spots for SRR32999268.sra
Read 601800 spots for SRR32999268.sra
Written 601800 spots for SRR32999268.sra
Read 601800 spots for SRR32999268.sra
Written 601800 spots for SRR32999268.sra
Read 601800 spots for SRR32999268.sra
Written 601800 spots for SRR32999268.sra
Read 601803 spots for SRR32999268.sra
Written 601803 spots for SRR32999268.sra
Read 601800 spots for SRR32999268.sra
Written 601800 spots for SRR32999268.sra
Read 601800 spots for SRR32999268.sra
Written 601800 spots for SRR32999268.sra
SRR ids: ['SRR32999268.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_tpmb3efs
SRR32999268.sra spots: 4814403
blocks: [[1, 601800], [601801, 1203600], [1203601, 1805400], [1805401, 2407200], [2407201, 3009000], [3009001, 3610800], [3610801, 4212600], [4212601, 4814403]]
SRR32999268 file size 1077544
SRR32999268 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32999268 SRR32999268_1.fastq SRR32999268_2.fastq
Input file:	SRR32999268_1.fastq
Paired file:	SRR32999268_2.fastq
trimmed:	SRR32999268-trimmed-pair1.fastq, SRR32999268-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu May 21 18:15:05 2026 >> started

Thu May 21 18:15:11 2026 >> done (5.762s)
4814403 read pairs processed; of these:
 230881 ( 4.80%) short read pairs filtered out after trimming by size control
   1403 ( 0.03%) empty read pairs filtered out after trimming by size control
4582119 (95.18%) read pairs available; of these:
2709541 (59.13%) trimmed read pairs available after processing
1872578 (40.87%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  42384	  0.92%
 19	  38325	  0.84%
 20	  41290	  0.90%
 21	  43785	  0.96%
 22	  45641	  1.00%
 23	  42208	  0.92%
 24	  44784	  0.98%
 25	  47864	  1.04%
 26	  44156	  0.96%
 27	  45488	  0.99%
 28	  44221	  0.97%
 29	  48126	  1.05%
 30	  51009	  1.11%
 31	  51244	  1.12%
 32	  44719	  0.98%
 33	  52017	  1.14%
 34	  51930	  1.13%
 35	  49688	  1.08%
 36	  50320	  1.10%
 37	  57932	  1.26%
 38	  50880	  1.11%
 39	  54446	  1.19%
 40	  69968	  1.53%
 41	  53009	  1.16%
 42	  53471	  1.17%
 43	 105293	  2.30%
 44	  67491	  1.47%
 45	  53365	  1.16%
 46	  51829	  1.13%
 47	  71528	  1.56%
 48	  83498	  1.82%
 49	  70834	  1.55%
 50	  52149	  1.14%
 51	  55372	  1.21%
 52	  59678	  1.30%
 53	  45768	  1.00%
 54	  52697	  1.15%
 55	  37755	  0.82%
 56	  34254	  0.75%
 57	  36149	  0.79%
 58	  44827	  0.98%
 59	  42452	  0.93%
 60	  35478	  0.77%
 61	  36404	  0.79%
 62	  43786	  0.96%
 63	  43966	  0.96%
 64	  34699	  0.76%
 65	  31237	  0.68%
 66	  28480	  0.62%
 67	  29874	  0.65%
 68	  29646	  0.65%
 69	  34994	  0.76%
 70	  30739	  0.67%
 71	  29774	  0.65%
 72	  34624	  0.76%
 73	  35299	  0.77%
 74	 147856	  3.23%
 75	 918422	 20.04%
 76	 852997	 18.62%
4582119 reads passed initial QC


criterion=sequence-density
sequence-density=4.93
sequence-density-rank=1
fanout-score=2.05
fanout-score-rank=22
prefix-density=4.51
prefix-fanout=2.0
sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG


criterion=fanout-score
sequence-density=1.09
sequence-density-rank=2
fanout-score=46.61
fanout-score-rank=1
prefix-density=1.30
prefix-fanout=39.2
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGATCTATCTCGTATGCCGT


criterion=sequence-density
sequence-density=3.26
sequence-density-rank=1
fanout-score=2.15
fanout-score-rank=30
prefix-density=6.74
prefix-fanout=1.0
sequence=CACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=42
fanout-score=856.53
fanout-score-rank=1
prefix-density=4.84
prefix-fanout=1.0
sequence=GTGTTAGGCCTTCCGCCAGCGTT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG -y CACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTA -o SRR32999268 SRR32999268_1.fastq SRR32999268_2.fastq
Input file:	SRR32999268_1.fastq
Paired file:	SRR32999268_2.fastq
trimmed:	SRR32999268-trimmed-pair1.fastq, SRR32999268-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACT
-- paired 3' end adapter sequence (-y):	CACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu May 21 18:15:23 2026 >> started

Thu May 21 18:15:26 2026 >> done (3.901s)
2749272 read pairs processed; of these:
   2756 ( 0.10%) short read pairs filtered out after trimming by size control
  16965 ( 0.62%) empty read pairs filtered out after trimming by size control
2729551 (99.28%) read pairs available; of these:
    232 ( 0.01%) trimmed read pairs available after processing
2729319 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  25235	  0.92%
 19	  22853	  0.84%
 20	  24763	  0.91%
 21	  26172	  0.96%
 22	  27377	  1.00%
 23	  25464	  0.93%
 24	  26799	  0.98%
 25	  28688	  1.05%
 26	  26422	  0.97%
 27	  27095	  0.99%
 28	  26567	  0.97%
 29	  28739	  1.05%
 30	  30398	  1.11%
 31	  30687	  1.12%
 32	  26550	  0.97%
 33	  30883	  1.13%
 34	  31174	  1.14%
 35	  29809	  1.09%
 36	  29817	  1.09%
 37	  34509	  1.26%
 38	  30306	  1.11%
 39	  32427	  1.19%
 40	  41836	  1.53%
 41	  31549	  1.16%
 42	  31883	  1.17%
 43	  62729	  2.30%
 44	  40002	  1.47%
 45	  31890	  1.17%
 46	  30994	  1.14%
 47	  42711	  1.56%
 48	  50024	  1.83%
 49	  42397	  1.55%
 50	  31155	  1.14%
 51	  33067	  1.21%
 52	  35590	  1.30%
 53	  27176	  1.00%
 54	  31613	  1.16%
 55	  22425	  0.82%
 56	  20349	  0.75%
 57	  21576	  0.79%
 58	  26594	  0.97%
 59	  25025	  0.92%
 60	  20969	  0.77%
 61	  21333	  0.78%
 62	  25943	  0.95%
 63	  26042	  0.95%
 64	  20520	  0.75%
 65	  18701	  0.69%
 66	  17028	  0.62%
 67	  17717	  0.65%
 68	  17564	  0.64%
 69	  20972	  0.77%
 70	  18221	  0.67%
 71	  17618	  0.65%
 72	  20621	  0.76%
 73	  21019	  0.77%
 74	  88222	  3.23%
 75	 546974	 20.04%
 76	 506768	 18.57%


criterion=sequence-density
sequence-density=4.70
sequence-density-rank=1
fanout-score=2.05
fanout-score-rank=23
prefix-density=4.52
prefix-fanout=2.1
sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG


criterion=fanout-score
sequence-density=1.16
sequence-density-rank=2
fanout-score=47.29
fanout-score-rank=1
prefix-density=1.39
prefix-fanout=39.5
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGATCTATCTCGTATGCCGT


criterion=sequence-density
sequence-density=3.27
sequence-density-rank=1
fanout-score=2.12
fanout-score-rank=29
prefix-density=6.64
prefix-fanout=1.0
sequence=CACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=39
fanout-score=856.89
fanout-score-rank=1
prefix-density=4.87
prefix-fanout=1.0
sequence=GTGTTAGGCCTACCGCCAGCGTT
SRR32999268 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 21 18:16:03
                             Started mapping on |	May 21 18:16:03
                                    Finished on |	May 21 18:19:59
       Mapping speed, Million of reads per hour |	69.60

                          Number of input reads |	4562398
                      Average input read length |	114
                                    UNIQUE READS:
                   Uniquely mapped reads number |	292398
                        Uniquely mapped reads % |	6.41%
                          Average mapped length |	121.08
                       Number of splices: Total |	37205
            Number of splices: Annotated (sjdb) |	8
                       Number of splices: GT/AG |	30863
                       Number of splices: GC/AG |	1108
                       Number of splices: AT/AC |	25
               Number of splices: Non-canonical |	5209
                      Mismatch rate per base, % |	0.88%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.18
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	3930031
             % of reads mapped to multiple loci |	86.14%
        Number of reads mapped to too many loci |	17866
             % of reads mapped to too many loci |	0.39%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.77%
                     % of reads unmapped: other |	2.29%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	339969	339969	339969
N_multimapping	3930031	3930031	3930031
N_noFeature	1667	10639	274925
N_ambiguous	17728	8370	2538
UnstrandedReadsAssigned:273003 PositiveStrandReadsAssigned:273389 NegativeStrandReadsAssigned:14935
Dataset is classified positive stranded
MeadianReadLen=62 20thPercentileLength=37 echo kmer=33
SRR32999268 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 4,322
[index] number of k-mers: 3,915,872
[index] number of equivalence classes: 4,486
[quant] running in paired-end mode
[quant] will process pair 1: SRR32999268-trimmed-pair1.fastq
                             SRR32999268-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,562,398 reads, 2,978 reads pseudoaligned
[quant] estimated average fragment length: 68.0396
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 52 rounds

  4323 SRR32999268.ke.tsv
  4497 SRR32999268.se.tsv
  8820 total
==> SRR32999268.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
AAC73112	66	19.8178	0	0
AAC73113	2463	2395.96	0	0
AAC73114	933	865.96	2	308.67
AAC73115	1287	1219.96	0	0
AAC73116	297	230.057	0	0
AAC73117	777	709.96	0	0
AAC73118	1431	1363.96	0	0
AAC73119	954	886.96	2	301.362
AAC73120	588	520.96	0	0

==> SRR32999268.se.tsv <==
b0001	0
b0002	1
b0003	2
b0004	1
b0005	0
b0006	0
b0007	1
b0008	3
b0009	0
b0010	0
SRR32999268 completed mapping pipeline successfully
